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RTN4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol RTN4
Entrez Gene ID 57142
Full Name reticulon 4
Synonyms ASY, NI220/250, NOGO, NOGO-A, NOGOC, NSP, NSP-CL, Nbla00271, Nbla10545, Nogo-B, Nogo-C, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C
General protein information
Preferred Names
reticulon-4
Names
reticulon-4
foocen
Human NogoA
reticulon 5
My043 protein
neurite outgrowth inhibitor
neurite growth inhibitor 220
neuroendocrine-specific protein C homolog
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2p16.3

Summary This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

604475


mRNA and Protein(s)

mRNA Protein Name
XM_005264434 XP_005264491 reticulon-4 isoform X1
XM_011532991 XP_011531293 reticulon-4 isoform X1
XM_011532992 XP_011531294 reticulon-4 isoform X1
NM_020532 NP_065393 reticulon-4 isoform A
NM_207520 NP_997403 reticulon-4 isoform D
NM_153828 NP_722550 reticulon-4 isoform B
NM_007008 NP_008939 reticulon-4 isoform C
NM_207521 NP_997404 reticulon-4 isoform E



Homo sapiens (human) RTN4 NP_065393.1
Macaca mulatta (Rhesus monkey) RTN4 XP_001112090.2
Canis lupus familiaris (dog) RTN4 XP_531828.2
Bos taurus (cattle) RTN4 NP_001106692.1
Mus musculus (house mouse) Rtn4 NP_918943.1
Rattus norvegicus (Norway rat) Rtn4 NP_114019.1
Gallus gallus (chicken) LOC101749579 XP_004940228.1
Xenopus (Silurana) tropicalis (western clawed frog) rtn4 NP_001161159.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RTN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RTN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu15795 XM_005264434 PREDICTED: Homo sapiens reticulon 4 (RTN4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu15795 XM_011532991 PREDICTED: Homo sapiens reticulon 4 (RTN4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu15795 XM_011532992 PREDICTED: Homo sapiens reticulon 4 (RTN4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu19329 NM_020532 Homo sapiens reticulon 4 (RTN4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $643.30
$919.00
OHu15978 NM_207520 Homo sapiens reticulon 4 (RTN4), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
OHu15728 NM_153828 Homo sapiens reticulon 4 (RTN4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00
OHu15762
NM_007008 Homo sapiens reticulon 4 (RTN4), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu15795 NM_207521 Homo sapiens reticulon 4 (RTN4), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu15795
Clone ID Related Accession (Same CDS sequence) NM_207521 , XM_005264434 , XM_011532991 , XM_011532992
Accession Version XM_005264434.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product reticulon-4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced gi:530367841. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGACTTGA AGGAGCAGCC AGGTAACACT ATTTCGGCTG GTCAAGAGGA TTTCCCATCT 
GTCCTGCTTG AAACTGCTGC TTCTCTTCCT TCTCTGTCTC CTCTCTCAGC CGCTTCTTTC
AAAGAACATG AATACCTTGG TAATTTGTCA ACAGTATTAC CCACTGAAGG AACACTTCAA
GAAAATGTCA GTGAAGCTTC TAAAGAGGTC TCAGAGAAGG CAAAAACTCT ACTCATAGAT
AGAGATTTAA CAGAGTTTTC AGAATTAGAA TACTCAGAAA TGGGATCATC GTTCAGTGTC
TCTCCAAAAG CAGAATCTGC CGTAATAGTA GCAAATCCTA GGGAAGAAAT AATCGTGAAA
AATAAAGATG AAGAAGAGAA GTTAGTTAGT AATAACATCC TTCATAATCA ACAAGAGTTA
CCTACAGCTC TTACTAAATT GGTTAAAGAG GATGAAGTTG TGTCTTCAGA AAAAGCAAAA
GACAGTTTTA ATGAAAAGAG AGTTGCAGTG GAAGCTCCTA TGAGGGAGGA ATATGCAGAC
TTCAAACCAT TTGAGCGAGT ATGGGAAGTG AAAGATAGTA AGGAAGATAG TGATATGTTG
GCTGCTGGAG GTAAAATCGA GAGCAACTTG GAAAGTAAAG TGGATAAAAA ATGTTTTGCA
GATAGCCTTG AGCAAACTAA TCACGAAAAA GATAGTGAGA GTAGTAATGA TGATACTTCT
TTCCCCAGTA CGCCAGAAGG TATAAAGGAT CGTTCAGGAG CATATATCAC ATGTGCTCCC
TTTAACCCAG CAGCAACTGA GAGCATTGCA ACAAACATTT TTCCTTTGTT AGGAGATCCT
ACTTCAGAAA ATAAGACCGA TGAAAAAAAA ATAGAAGAAA AGAAGGCCCA AATAGTAACA
GAGAAGAATA CTAGCACCAA AACATCAAAC CCTTTTCTTG TAGCAGCACA GGATTCTGAG
ACAGATTATG TCACAACAGA TAATTTAACA AAGGTGACTG AGGAAGTCGT GGCAAACATG
CCTGAAGGCC TGACTCCAGA TTTAGTACAG GAAGCATGTG AAAGTGAATT GAATGAAGTT
ACTGGTACAA AGATTGCTTA TGAAACAAAA ATGGACTTGG TTCAAACATC AGAAGTTATG
CAAGAGTCAC TCTATCCTGC AGCACAGCTT TGCCCATCAT TTGAAGAGTC AGAAGCTACT
CCTTCACCAG TTTTGCCTGA CATTGTTATG GAAGCACCAT TGAATTCTGC AGTTCCTAGT
GCTGGTGCTT CCGTGATACA GCCCAGCTCA TCACCATTAG AAGCTTCTTC AGTTAATTAT
GAAAGCATAA AACATGAGCC TGAAAACCCC CCACCATATG AAGAGGCCAT GAGTGTATCA
CTAAAAAAAG TATCAGGAAT AAAGGAAGAA ATTAAAGAGC CTGAAAATAT TAATGCAGCT
CTTCAAGAAA CAGAAGCTCC TTATATATCT ATTGCATGTG ATTTAATTAA AGAAACAAAG
CTTTCTGCTG AACCAGCTCC GGATTTCTCT GATTATTCAG AAATGGCAAA AGTTGAACAG
CCAGTGCCTG ATCATTCTGA GCTAGTTGAA GATTCCTCAC CTGATTCTGA ACCAGTTGAC
TTATTTAGTG ATGATTCAAT ACCTGACGTT CCACAAAAAC AAGATGAAAC TGTGATGCTT
GTGAAAGAAA GTCTCACTGA GACTTCATTT GAGTCAATGA TAGAATATGA AAATAAGGAA
AAACTCAGTG CTTTGCCACC TGAGGGAGGA AAGCCATATT TGGAATCTTT TAAGCTCAGT
TTAGATAACA CAAAAGATAC CCTGTTACCT GATGAAGTTT CAACATTGAG CAAAAAGGAG
AAAATTCCTT TGCAGATGGA GGAGCTCAGT ACTGCAGTTT ATTCAAATGA TGACTTATTT
ATTTCTAAGG AAGCACAGAT AAGAGAAACT GAAACGTTTT CAGATTCATC TCCAATTGAA
ATTATAGATG AGTTCCCTAC ATTGATCAGT TCTAAAACTG ATTCATTTTC TAAATTAGCC
AGGGAATATA CTGACCTAGA AGTATCCCAC AAAAGTGAAA TTGCTAATGC CCCGGATGGA
GCTGGGTCAT TGCCTTGCAC AGAATTGCCC CATGACCTTT CTTTGAAGAA CATACAACCC
AAAGTTGAAG AGAAAATCAG TTTCTCAGAT GACTTTTCTA AAAATGGGTC TGCTACATCA
AAGGTGCTCT TATTGCCTCC AGATGTTTCT GCTTTGGCCA CTCAAGCAGA GATAGAGAGC
ATAGTTAAAC CCAAAGTTCT TGTGAAAGAA GCTGAGAAAA AACTTCCTTC CGATACAGAA
AAAGAGGACA GATCACCATC TGCTATATTT TCAGCAGAGC TGAGTAAAAC TTCAGTTGTT
GACCTCCTGT ACTGGAGAGA CATTAAGAAG ACTGGAGTGG TGTTTGGTGC CAGCCTATTC
CTGCTGCTTT CATTGACAGT ATTCAGCATT GTGAGCGTAA CAGCCTACAT TGCCTTGGCC
CTGCTCTCTG TGACCATCAG CTTTAGGATA TACAAGGGTG TGATCCAAGC TATCCAGAAA
TCAGATGAAG GCCACCCATT CAGGGCATAT CTGGAATCTG AAGTTGCTAT ATCTGAGGAG
TTGGTTCAGA AGTACAGTAA TTCTGCTCTT GGTCATGTGA ACTGCACGAT AAAGGAACTC
AGGCGCCTCT TCTTAGTTGA TGATTTAGTT GATTCTCTGA AGTTTGCAGT GTTGATGTGG
GTATTTACCT ATGTTGGTGC CTTGTTTAAT GGTCTGACAC TACTGATTTT GGCTCTCATT
TCACTCTTCA GTGTTCCTGT TATTTATGAA CGGCATCAGG CACAGATAGA TCATTATCTA
GGACTTGCAA ATAAGAATGT TAAAGATGCT ATGGCTAAAA TCCAAGCAAA AATCCCTGGA
TTGAAGCGCA AAGCTGAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005264491.1
CDS311..3271
Misc Feature(1)2705..3217(+)
Translation

Target ORF information:

RefSeq Version XM_005264434
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens reticulon 4 (RTN4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005264434

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGACTTGA AGGAGCAGCC AGGTAACACT ATTTCGGCTG GTCAAGAGGA TTTCCCATCT 
GTCCTGCTTG AAACTGCTGC TTCTCTTCCT TCTCTGTCTC CTCTCTCAGC CGCTTCTTTC
AAAGAACATG AATACCTTGG TAATTTGTCA ACAGTATTAC CCACTGAAGG AACACTTCAA
GAAAATGTCA GTGAAGCTTC TAAAGAGGTC TCAGAGAAGG CAAAAACTCT ACTCATAGAT
AGAGATTTAA CAGAGTTTTC AGAATTAGAA TACTCAGAAA TGGGATCATC GTTCAGTGTC
TCTCCAAAAG CAGAATCTGC CGTAATAGTA GCAAATCCTA GGGAAGAAAT AATCGTGAAA
AATAAAGATG AAGAAGAGAA GTTAGTTAGT AATAACATCC TTCATAATCA ACAAGAGTTA
CCTACAGCTC TTACTAAATT GGTTAAAGAG GATGAAGTTG TGTCTTCAGA AAAAGCAAAA
GACAGTTTTA ATGAAAAGAG AGTTGCAGTG GAAGCTCCTA TGAGGGAGGA ATATGCAGAC
TTCAAACCAT TTGAGCGAGT ATGGGAAGTG AAAGATAGTA AGGAAGATAG TGATATGTTG
GCTGCTGGAG GTAAAATCGA GAGCAACTTG GAAAGTAAAG TGGATAAAAA ATGTTTTGCA
GATAGCCTTG AGCAAACTAA TCACGAAAAA GATAGTGAGA GTAGTAATGA TGATACTTCT
TTCCCCAGTA CGCCAGAAGG TATAAAGGAT CGTTCAGGAG CATATATCAC ATGTGCTCCC
TTTAACCCAG CAGCAACTGA GAGCATTGCA ACAAACATTT TTCCTTTGTT AGGAGATCCT
ACTTCAGAAA ATAAGACCGA TGAAAAAAAA ATAGAAGAAA AGAAGGCCCA AATAGTAACA
GAGAAGAATA CTAGCACCAA AACATCAAAC CCTTTTCTTG TAGCAGCACA GGATTCTGAG
ACAGATTATG TCACAACAGA TAATTTAACA AAGGTGACTG AGGAAGTCGT GGCAAACATG
CCTGAAGGCC TGACTCCAGA TTTAGTACAG GAAGCATGTG AAAGTGAATT GAATGAAGTT
ACTGGTACAA AGATTGCTTA TGAAACAAAA ATGGACTTGG TTCAAACATC AGAAGTTATG
CAAGAGTCAC TCTATCCTGC AGCACAGCTT TGCCCATCAT TTGAAGAGTC AGAAGCTACT
CCTTCACCAG TTTTGCCTGA CATTGTTATG GAAGCACCAT TGAATTCTGC AGTTCCTAGT
GCTGGTGCTT CCGTGATACA GCCCAGCTCA TCACCATTAG AAGCTTCTTC AGTTAATTAT
GAAAGCATAA AACATGAGCC TGAAAACCCC CCACCATATG AAGAGGCCAT GAGTGTATCA
CTAAAAAAAG TATCAGGAAT AAAGGAAGAA ATTAAAGAGC CTGAAAATAT TAATGCAGCT
CTTCAAGAAA CAGAAGCTCC TTATATATCT ATTGCATGTG ATTTAATTAA AGAAACAAAG
CTTTCTGCTG AACCAGCTCC GGATTTCTCT GATTATTCAG AAATGGCAAA AGTTGAACAG
CCAGTGCCTG ATCATTCTGA GCTAGTTGAA GATTCCTCAC CTGATTCTGA ACCAGTTGAC
TTATTTAGTG ATGATTCAAT ACCTGACGTT CCACAAAAAC AAGATGAAAC TGTGATGCTT
GTGAAAGAAA GTCTCACTGA GACTTCATTT GAGTCAATGA TAGAATATGA AAATAAGGAA
AAACTCAGTG CTTTGCCACC TGAGGGAGGA AAGCCATATT TGGAATCTTT TAAGCTCAGT
TTAGATAACA CAAAAGATAC CCTGTTACCT GATGAAGTTT CAACATTGAG CAAAAAGGAG
AAAATTCCTT TGCAGATGGA GGAGCTCAGT ACTGCAGTTT ATTCAAATGA TGACTTATTT
ATTTCTAAGG AAGCACAGAT AAGAGAAACT GAAACGTTTT CAGATTCATC TCCAATTGAA
ATTATAGATG AGTTCCCTAC ATTGATCAGT TCTAAAACTG ATTCATTTTC TAAATTAGCC
AGGGAATATA CTGACCTAGA AGTATCCCAC AAAAGTGAAA TTGCTAATGC CCCGGATGGA
GCTGGGTCAT TGCCTTGCAC AGAATTGCCC CATGACCTTT CTTTGAAGAA CATACAACCC
AAAGTTGAAG AGAAAATCAG TTTCTCAGAT GACTTTTCTA AAAATGGGTC TGCTACATCA
AAGGTGCTCT TATTGCCTCC AGATGTTTCT GCTTTGGCCA CTCAAGCAGA GATAGAGAGC
ATAGTTAAAC CCAAAGTTCT TGTGAAAGAA GCTGAGAAAA AACTTCCTTC CGATACAGAA
AAAGAGGACA GATCACCATC TGCTATATTT TCAGCAGAGC TGAGTAAAAC TTCAGTTGTT
GACCTCCTGT ACTGGAGAGA CATTAAGAAG ACTGGAGTGG TGTTTGGTGC CAGCCTATTC
CTGCTGCTTT CATTGACAGT ATTCAGCATT GTGAGCGTAA CAGCCTACAT TGCCTTGGCC
CTGCTCTCTG TGACCATCAG CTTTAGGATA TACAAGGGTG TGATCCAAGC TATCCAGAAA
TCAGATGAAG GCCACCCATT CAGGGCATAT CTGGAATCTG AAGTTGCTAT ATCTGAGGAG
TTGGTTCAGA AGTACAGTAA TTCTGCTCTT GGTCATGTGA ACTGCACGAT AAAGGAACTC
AGGCGCCTCT TCTTAGTTGA TGATTTAGTT GATTCTCTGA AGTTTGCAGT GTTGATGTGG
GTATTTACCT ATGTTGGTGC CTTGTTTAAT GGTCTGACAC TACTGATTTT GGCTCTCATT
TCACTCTTCA GTGTTCCTGT TATTTATGAA CGGCATCAGG CACAGATAGA TCATTATCTA
GGACTTGCAA ATAAGAATGT TAAAGATGCT ATGGCTAAAA TCCAAGCAAA AATCCCTGGA
TTGAAGCGCA AAGCTGAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15795
Clone ID Related Accession (Same CDS sequence) NM_207521 , XM_005264434 , XM_011532991 , XM_011532992
Accession Version XM_011532991.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product reticulon-4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGACTTGA AGGAGCAGCC AGGTAACACT ATTTCGGCTG GTCAAGAGGA TTTCCCATCT 
GTCCTGCTTG AAACTGCTGC TTCTCTTCCT TCTCTGTCTC CTCTCTCAGC CGCTTCTTTC
AAAGAACATG AATACCTTGG TAATTTGTCA ACAGTATTAC CCACTGAAGG AACACTTCAA
GAAAATGTCA GTGAAGCTTC TAAAGAGGTC TCAGAGAAGG CAAAAACTCT ACTCATAGAT
AGAGATTTAA CAGAGTTTTC AGAATTAGAA TACTCAGAAA TGGGATCATC GTTCAGTGTC
TCTCCAAAAG CAGAATCTGC CGTAATAGTA GCAAATCCTA GGGAAGAAAT AATCGTGAAA
AATAAAGATG AAGAAGAGAA GTTAGTTAGT AATAACATCC TTCATAATCA ACAAGAGTTA
CCTACAGCTC TTACTAAATT GGTTAAAGAG GATGAAGTTG TGTCTTCAGA AAAAGCAAAA
GACAGTTTTA ATGAAAAGAG AGTTGCAGTG GAAGCTCCTA TGAGGGAGGA ATATGCAGAC
TTCAAACCAT TTGAGCGAGT ATGGGAAGTG AAAGATAGTA AGGAAGATAG TGATATGTTG
GCTGCTGGAG GTAAAATCGA GAGCAACTTG GAAAGTAAAG TGGATAAAAA ATGTTTTGCA
GATAGCCTTG AGCAAACTAA TCACGAAAAA GATAGTGAGA GTAGTAATGA TGATACTTCT
TTCCCCAGTA CGCCAGAAGG TATAAAGGAT CGTTCAGGAG CATATATCAC ATGTGCTCCC
TTTAACCCAG CAGCAACTGA GAGCATTGCA ACAAACATTT TTCCTTTGTT AGGAGATCCT
ACTTCAGAAA ATAAGACCGA TGAAAAAAAA ATAGAAGAAA AGAAGGCCCA AATAGTAACA
GAGAAGAATA CTAGCACCAA AACATCAAAC CCTTTTCTTG TAGCAGCACA GGATTCTGAG
ACAGATTATG TCACAACAGA TAATTTAACA AAGGTGACTG AGGAAGTCGT GGCAAACATG
CCTGAAGGCC TGACTCCAGA TTTAGTACAG GAAGCATGTG AAAGTGAATT GAATGAAGTT
ACTGGTACAA AGATTGCTTA TGAAACAAAA ATGGACTTGG TTCAAACATC AGAAGTTATG
CAAGAGTCAC TCTATCCTGC AGCACAGCTT TGCCCATCAT TTGAAGAGTC AGAAGCTACT
CCTTCACCAG TTTTGCCTGA CATTGTTATG GAAGCACCAT TGAATTCTGC AGTTCCTAGT
GCTGGTGCTT CCGTGATACA GCCCAGCTCA TCACCATTAG AAGCTTCTTC AGTTAATTAT
GAAAGCATAA AACATGAGCC TGAAAACCCC CCACCATATG AAGAGGCCAT GAGTGTATCA
CTAAAAAAAG TATCAGGAAT AAAGGAAGAA ATTAAAGAGC CTGAAAATAT TAATGCAGCT
CTTCAAGAAA CAGAAGCTCC TTATATATCT ATTGCATGTG ATTTAATTAA AGAAACAAAG
CTTTCTGCTG AACCAGCTCC GGATTTCTCT GATTATTCAG AAATGGCAAA AGTTGAACAG
CCAGTGCCTG ATCATTCTGA GCTAGTTGAA GATTCCTCAC CTGATTCTGA ACCAGTTGAC
TTATTTAGTG ATGATTCAAT ACCTGACGTT CCACAAAAAC AAGATGAAAC TGTGATGCTT
GTGAAAGAAA GTCTCACTGA GACTTCATTT GAGTCAATGA TAGAATATGA AAATAAGGAA
AAACTCAGTG CTTTGCCACC TGAGGGAGGA AAGCCATATT TGGAATCTTT TAAGCTCAGT
TTAGATAACA CAAAAGATAC CCTGTTACCT GATGAAGTTT CAACATTGAG CAAAAAGGAG
AAAATTCCTT TGCAGATGGA GGAGCTCAGT ACTGCAGTTT ATTCAAATGA TGACTTATTT
ATTTCTAAGG AAGCACAGAT AAGAGAAACT GAAACGTTTT CAGATTCATC TCCAATTGAA
ATTATAGATG AGTTCCCTAC ATTGATCAGT TCTAAAACTG ATTCATTTTC TAAATTAGCC
AGGGAATATA CTGACCTAGA AGTATCCCAC AAAAGTGAAA TTGCTAATGC CCCGGATGGA
GCTGGGTCAT TGCCTTGCAC AGAATTGCCC CATGACCTTT CTTTGAAGAA CATACAACCC
AAAGTTGAAG AGAAAATCAG TTTCTCAGAT GACTTTTCTA AAAATGGGTC TGCTACATCA
AAGGTGCTCT TATTGCCTCC AGATGTTTCT GCTTTGGCCA CTCAAGCAGA GATAGAGAGC
ATAGTTAAAC CCAAAGTTCT TGTGAAAGAA GCTGAGAAAA AACTTCCTTC CGATACAGAA
AAAGAGGACA GATCACCATC TGCTATATTT TCAGCAGAGC TGAGTAAAAC TTCAGTTGTT
GACCTCCTGT ACTGGAGAGA CATTAAGAAG ACTGGAGTGG TGTTTGGTGC CAGCCTATTC
CTGCTGCTTT CATTGACAGT ATTCAGCATT GTGAGCGTAA CAGCCTACAT TGCCTTGGCC
CTGCTCTCTG TGACCATCAG CTTTAGGATA TACAAGGGTG TGATCCAAGC TATCCAGAAA
TCAGATGAAG GCCACCCATT CAGGGCATAT CTGGAATCTG AAGTTGCTAT ATCTGAGGAG
TTGGTTCAGA AGTACAGTAA TTCTGCTCTT GGTCATGTGA ACTGCACGAT AAAGGAACTC
AGGCGCCTCT TCTTAGTTGA TGATTTAGTT GATTCTCTGA AGTTTGCAGT GTTGATGTGG
GTATTTACCT ATGTTGGTGC CTTGTTTAAT GGTCTGACAC TACTGATTTT GGCTCTCATT
TCACTCTTCA GTGTTCCTGT TATTTATGAA CGGCATCAGG CACAGATAGA TCATTATCTA
GGACTTGCAA ATAAGAATGT TAAAGATGCT ATGGCTAAAA TCCAAGCAAA AATCCCTGGA
TTGAAGCGCA AAGCTGAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011531293.1
CDS179..3139
Misc Feature(1)2573..3085(+)
Translation

Target ORF information:

RefSeq Version XM_011532991
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens reticulon 4 (RTN4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011532991

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGACTTGA AGGAGCAGCC AGGTAACACT ATTTCGGCTG GTCAAGAGGA TTTCCCATCT 
GTCCTGCTTG AAACTGCTGC TTCTCTTCCT TCTCTGTCTC CTCTCTCAGC CGCTTCTTTC
AAAGAACATG AATACCTTGG TAATTTGTCA ACAGTATTAC CCACTGAAGG AACACTTCAA
GAAAATGTCA GTGAAGCTTC TAAAGAGGTC TCAGAGAAGG CAAAAACTCT ACTCATAGAT
AGAGATTTAA CAGAGTTTTC AGAATTAGAA TACTCAGAAA TGGGATCATC GTTCAGTGTC
TCTCCAAAAG CAGAATCTGC CGTAATAGTA GCAAATCCTA GGGAAGAAAT AATCGTGAAA
AATAAAGATG AAGAAGAGAA GTTAGTTAGT AATAACATCC TTCATAATCA ACAAGAGTTA
CCTACAGCTC TTACTAAATT GGTTAAAGAG GATGAAGTTG TGTCTTCAGA AAAAGCAAAA
GACAGTTTTA ATGAAAAGAG AGTTGCAGTG GAAGCTCCTA TGAGGGAGGA ATATGCAGAC
TTCAAACCAT TTGAGCGAGT ATGGGAAGTG AAAGATAGTA AGGAAGATAG TGATATGTTG
GCTGCTGGAG GTAAAATCGA GAGCAACTTG GAAAGTAAAG TGGATAAAAA ATGTTTTGCA
GATAGCCTTG AGCAAACTAA TCACGAAAAA GATAGTGAGA GTAGTAATGA TGATACTTCT
TTCCCCAGTA CGCCAGAAGG TATAAAGGAT CGTTCAGGAG CATATATCAC ATGTGCTCCC
TTTAACCCAG CAGCAACTGA GAGCATTGCA ACAAACATTT TTCCTTTGTT AGGAGATCCT
ACTTCAGAAA ATAAGACCGA TGAAAAAAAA ATAGAAGAAA AGAAGGCCCA AATAGTAACA
GAGAAGAATA CTAGCACCAA AACATCAAAC CCTTTTCTTG TAGCAGCACA GGATTCTGAG
ACAGATTATG TCACAACAGA TAATTTAACA AAGGTGACTG AGGAAGTCGT GGCAAACATG
CCTGAAGGCC TGACTCCAGA TTTAGTACAG GAAGCATGTG AAAGTGAATT GAATGAAGTT
ACTGGTACAA AGATTGCTTA TGAAACAAAA ATGGACTTGG TTCAAACATC AGAAGTTATG
CAAGAGTCAC TCTATCCTGC AGCACAGCTT TGCCCATCAT TTGAAGAGTC AGAAGCTACT
CCTTCACCAG TTTTGCCTGA CATTGTTATG GAAGCACCAT TGAATTCTGC AGTTCCTAGT
GCTGGTGCTT CCGTGATACA GCCCAGCTCA TCACCATTAG AAGCTTCTTC AGTTAATTAT
GAAAGCATAA AACATGAGCC TGAAAACCCC CCACCATATG AAGAGGCCAT GAGTGTATCA
CTAAAAAAAG TATCAGGAAT AAAGGAAGAA ATTAAAGAGC CTGAAAATAT TAATGCAGCT
CTTCAAGAAA CAGAAGCTCC TTATATATCT ATTGCATGTG ATTTAATTAA AGAAACAAAG
CTTTCTGCTG AACCAGCTCC GGATTTCTCT GATTATTCAG AAATGGCAAA AGTTGAACAG
CCAGTGCCTG ATCATTCTGA GCTAGTTGAA GATTCCTCAC CTGATTCTGA ACCAGTTGAC
TTATTTAGTG ATGATTCAAT ACCTGACGTT CCACAAAAAC AAGATGAAAC TGTGATGCTT
GTGAAAGAAA GTCTCACTGA GACTTCATTT GAGTCAATGA TAGAATATGA AAATAAGGAA
AAACTCAGTG CTTTGCCACC TGAGGGAGGA AAGCCATATT TGGAATCTTT TAAGCTCAGT
TTAGATAACA CAAAAGATAC CCTGTTACCT GATGAAGTTT CAACATTGAG CAAAAAGGAG
AAAATTCCTT TGCAGATGGA GGAGCTCAGT ACTGCAGTTT ATTCAAATGA TGACTTATTT
ATTTCTAAGG AAGCACAGAT AAGAGAAACT GAAACGTTTT CAGATTCATC TCCAATTGAA
ATTATAGATG AGTTCCCTAC ATTGATCAGT TCTAAAACTG ATTCATTTTC TAAATTAGCC
AGGGAATATA CTGACCTAGA AGTATCCCAC AAAAGTGAAA TTGCTAATGC CCCGGATGGA
GCTGGGTCAT TGCCTTGCAC AGAATTGCCC CATGACCTTT CTTTGAAGAA CATACAACCC
AAAGTTGAAG AGAAAATCAG TTTCTCAGAT GACTTTTCTA AAAATGGGTC TGCTACATCA
AAGGTGCTCT TATTGCCTCC AGATGTTTCT GCTTTGGCCA CTCAAGCAGA GATAGAGAGC
ATAGTTAAAC CCAAAGTTCT TGTGAAAGAA GCTGAGAAAA AACTTCCTTC CGATACAGAA
AAAGAGGACA GATCACCATC TGCTATATTT TCAGCAGAGC TGAGTAAAAC TTCAGTTGTT
GACCTCCTGT ACTGGAGAGA CATTAAGAAG ACTGGAGTGG TGTTTGGTGC CAGCCTATTC
CTGCTGCTTT CATTGACAGT ATTCAGCATT GTGAGCGTAA CAGCCTACAT TGCCTTGGCC
CTGCTCTCTG TGACCATCAG CTTTAGGATA TACAAGGGTG TGATCCAAGC TATCCAGAAA
TCAGATGAAG GCCACCCATT CAGGGCATAT CTGGAATCTG AAGTTGCTAT ATCTGAGGAG
TTGGTTCAGA AGTACAGTAA TTCTGCTCTT GGTCATGTGA ACTGCACGAT AAAGGAACTC
AGGCGCCTCT TCTTAGTTGA TGATTTAGTT GATTCTCTGA AGTTTGCAGT GTTGATGTGG
GTATTTACCT ATGTTGGTGC CTTGTTTAAT GGTCTGACAC TACTGATTTT GGCTCTCATT
TCACTCTTCA GTGTTCCTGT TATTTATGAA CGGCATCAGG CACAGATAGA TCATTATCTA
GGACTTGCAA ATAAGAATGT TAAAGATGCT ATGGCTAAAA TCCAAGCAAA AATCCCTGGA
TTGAAGCGCA AAGCTGAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15795
Clone ID Related Accession (Same CDS sequence) NM_207521 , XM_005264434 , XM_011532991 , XM_011532992
Accession Version XM_011532992.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product reticulon-4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_022184.16) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGACTTGA AGGAGCAGCC AGGTAACACT ATTTCGGCTG GTCAAGAGGA TTTCCCATCT 
GTCCTGCTTG AAACTGCTGC TTCTCTTCCT TCTCTGTCTC CTCTCTCAGC CGCTTCTTTC
AAAGAACATG AATACCTTGG TAATTTGTCA ACAGTATTAC CCACTGAAGG AACACTTCAA
GAAAATGTCA GTGAAGCTTC TAAAGAGGTC TCAGAGAAGG CAAAAACTCT ACTCATAGAT
AGAGATTTAA CAGAGTTTTC AGAATTAGAA TACTCAGAAA TGGGATCATC GTTCAGTGTC
TCTCCAAAAG CAGAATCTGC CGTAATAGTA GCAAATCCTA GGGAAGAAAT AATCGTGAAA
AATAAAGATG AAGAAGAGAA GTTAGTTAGT AATAACATCC TTCATAATCA ACAAGAGTTA
CCTACAGCTC TTACTAAATT GGTTAAAGAG GATGAAGTTG TGTCTTCAGA AAAAGCAAAA
GACAGTTTTA ATGAAAAGAG AGTTGCAGTG GAAGCTCCTA TGAGGGAGGA ATATGCAGAC
TTCAAACCAT TTGAGCGAGT ATGGGAAGTG AAAGATAGTA AGGAAGATAG TGATATGTTG
GCTGCTGGAG GTAAAATCGA GAGCAACTTG GAAAGTAAAG TGGATAAAAA ATGTTTTGCA
GATAGCCTTG AGCAAACTAA TCACGAAAAA GATAGTGAGA GTAGTAATGA TGATACTTCT
TTCCCCAGTA CGCCAGAAGG TATAAAGGAT CGTTCAGGAG CATATATCAC ATGTGCTCCC
TTTAACCCAG CAGCAACTGA GAGCATTGCA ACAAACATTT TTCCTTTGTT AGGAGATCCT
ACTTCAGAAA ATAAGACCGA TGAAAAAAAA ATAGAAGAAA AGAAGGCCCA AATAGTAACA
GAGAAGAATA CTAGCACCAA AACATCAAAC CCTTTTCTTG TAGCAGCACA GGATTCTGAG
ACAGATTATG TCACAACAGA TAATTTAACA AAGGTGACTG AGGAAGTCGT GGCAAACATG
CCTGAAGGCC TGACTCCAGA TTTAGTACAG GAAGCATGTG AAAGTGAATT GAATGAAGTT
ACTGGTACAA AGATTGCTTA TGAAACAAAA ATGGACTTGG TTCAAACATC AGAAGTTATG
CAAGAGTCAC TCTATCCTGC AGCACAGCTT TGCCCATCAT TTGAAGAGTC AGAAGCTACT
CCTTCACCAG TTTTGCCTGA CATTGTTATG GAAGCACCAT TGAATTCTGC AGTTCCTAGT
GCTGGTGCTT CCGTGATACA GCCCAGCTCA TCACCATTAG AAGCTTCTTC AGTTAATTAT
GAAAGCATAA AACATGAGCC TGAAAACCCC CCACCATATG AAGAGGCCAT GAGTGTATCA
CTAAAAAAAG TATCAGGAAT AAAGGAAGAA ATTAAAGAGC CTGAAAATAT TAATGCAGCT
CTTCAAGAAA CAGAAGCTCC TTATATATCT ATTGCATGTG ATTTAATTAA AGAAACAAAG
CTTTCTGCTG AACCAGCTCC GGATTTCTCT GATTATTCAG AAATGGCAAA AGTTGAACAG
CCAGTGCCTG ATCATTCTGA GCTAGTTGAA GATTCCTCAC CTGATTCTGA ACCAGTTGAC
TTATTTAGTG ATGATTCAAT ACCTGACGTT CCACAAAAAC AAGATGAAAC TGTGATGCTT
GTGAAAGAAA GTCTCACTGA GACTTCATTT GAGTCAATGA TAGAATATGA AAATAAGGAA
AAACTCAGTG CTTTGCCACC TGAGGGAGGA AAGCCATATT TGGAATCTTT TAAGCTCAGT
TTAGATAACA CAAAAGATAC CCTGTTACCT GATGAAGTTT CAACATTGAG CAAAAAGGAG
AAAATTCCTT TGCAGATGGA GGAGCTCAGT ACTGCAGTTT ATTCAAATGA TGACTTATTT
ATTTCTAAGG AAGCACAGAT AAGAGAAACT GAAACGTTTT CAGATTCATC TCCAATTGAA
ATTATAGATG AGTTCCCTAC ATTGATCAGT TCTAAAACTG ATTCATTTTC TAAATTAGCC
AGGGAATATA CTGACCTAGA AGTATCCCAC AAAAGTGAAA TTGCTAATGC CCCGGATGGA
GCTGGGTCAT TGCCTTGCAC AGAATTGCCC CATGACCTTT CTTTGAAGAA CATACAACCC
AAAGTTGAAG AGAAAATCAG TTTCTCAGAT GACTTTTCTA AAAATGGGTC TGCTACATCA
AAGGTGCTCT TATTGCCTCC AGATGTTTCT GCTTTGGCCA CTCAAGCAGA GATAGAGAGC
ATAGTTAAAC CCAAAGTTCT TGTGAAAGAA GCTGAGAAAA AACTTCCTTC CGATACAGAA
AAAGAGGACA GATCACCATC TGCTATATTT TCAGCAGAGC TGAGTAAAAC TTCAGTTGTT
GACCTCCTGT ACTGGAGAGA CATTAAGAAG ACTGGAGTGG TGTTTGGTGC CAGCCTATTC
CTGCTGCTTT CATTGACAGT ATTCAGCATT GTGAGCGTAA CAGCCTACAT TGCCTTGGCC
CTGCTCTCTG TGACCATCAG CTTTAGGATA TACAAGGGTG TGATCCAAGC TATCCAGAAA
TCAGATGAAG GCCACCCATT CAGGGCATAT CTGGAATCTG AAGTTGCTAT ATCTGAGGAG
TTGGTTCAGA AGTACAGTAA TTCTGCTCTT GGTCATGTGA ACTGCACGAT AAAGGAACTC
AGGCGCCTCT TCTTAGTTGA TGATTTAGTT GATTCTCTGA AGTTTGCAGT GTTGATGTGG
GTATTTACCT ATGTTGGTGC CTTGTTTAAT GGTCTGACAC TACTGATTTT GGCTCTCATT
TCACTCTTCA GTGTTCCTGT TATTTATGAA CGGCATCAGG CACAGATAGA TCATTATCTA
GGACTTGCAA ATAAGAATGT TAAAGATGCT ATGGCTAAAA TCCAAGCAAA AATCCCTGGA
TTGAAGCGCA AAGCTGAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011531294.1
CDS170..3130
Misc Feature(1)2564..3076(+)
Translation

Target ORF information:

RefSeq Version XM_011532992
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens reticulon 4 (RTN4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011532992

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGACTTGA AGGAGCAGCC AGGTAACACT ATTTCGGCTG GTCAAGAGGA TTTCCCATCT 
GTCCTGCTTG AAACTGCTGC TTCTCTTCCT TCTCTGTCTC CTCTCTCAGC CGCTTCTTTC
AAAGAACATG AATACCTTGG TAATTTGTCA ACAGTATTAC CCACTGAAGG AACACTTCAA
GAAAATGTCA GTGAAGCTTC TAAAGAGGTC TCAGAGAAGG CAAAAACTCT ACTCATAGAT
AGAGATTTAA CAGAGTTTTC AGAATTAGAA TACTCAGAAA TGGGATCATC GTTCAGTGTC
TCTCCAAAAG CAGAATCTGC CGTAATAGTA GCAAATCCTA GGGAAGAAAT AATCGTGAAA
AATAAAGATG AAGAAGAGAA GTTAGTTAGT AATAACATCC TTCATAATCA ACAAGAGTTA
CCTACAGCTC TTACTAAATT GGTTAAAGAG GATGAAGTTG TGTCTTCAGA AAAAGCAAAA
GACAGTTTTA ATGAAAAGAG AGTTGCAGTG GAAGCTCCTA TGAGGGAGGA ATATGCAGAC
TTCAAACCAT TTGAGCGAGT ATGGGAAGTG AAAGATAGTA AGGAAGATAG TGATATGTTG
GCTGCTGGAG GTAAAATCGA GAGCAACTTG GAAAGTAAAG TGGATAAAAA ATGTTTTGCA
GATAGCCTTG AGCAAACTAA TCACGAAAAA GATAGTGAGA GTAGTAATGA TGATACTTCT
TTCCCCAGTA CGCCAGAAGG TATAAAGGAT CGTTCAGGAG CATATATCAC ATGTGCTCCC
TTTAACCCAG CAGCAACTGA GAGCATTGCA ACAAACATTT TTCCTTTGTT AGGAGATCCT
ACTTCAGAAA ATAAGACCGA TGAAAAAAAA ATAGAAGAAA AGAAGGCCCA AATAGTAACA
GAGAAGAATA CTAGCACCAA AACATCAAAC CCTTTTCTTG TAGCAGCACA GGATTCTGAG
ACAGATTATG TCACAACAGA TAATTTAACA AAGGTGACTG AGGAAGTCGT GGCAAACATG
CCTGAAGGCC TGACTCCAGA TTTAGTACAG GAAGCATGTG AAAGTGAATT GAATGAAGTT
ACTGGTACAA AGATTGCTTA TGAAACAAAA ATGGACTTGG TTCAAACATC AGAAGTTATG
CAAGAGTCAC TCTATCCTGC AGCACAGCTT TGCCCATCAT TTGAAGAGTC AGAAGCTACT
CCTTCACCAG TTTTGCCTGA CATTGTTATG GAAGCACCAT TGAATTCTGC AGTTCCTAGT
GCTGGTGCTT CCGTGATACA GCCCAGCTCA TCACCATTAG AAGCTTCTTC AGTTAATTAT
GAAAGCATAA AACATGAGCC TGAAAACCCC CCACCATATG AAGAGGCCAT GAGTGTATCA
CTAAAAAAAG TATCAGGAAT AAAGGAAGAA ATTAAAGAGC CTGAAAATAT TAATGCAGCT
CTTCAAGAAA CAGAAGCTCC TTATATATCT ATTGCATGTG ATTTAATTAA AGAAACAAAG
CTTTCTGCTG AACCAGCTCC GGATTTCTCT GATTATTCAG AAATGGCAAA AGTTGAACAG
CCAGTGCCTG ATCATTCTGA GCTAGTTGAA GATTCCTCAC CTGATTCTGA ACCAGTTGAC
TTATTTAGTG ATGATTCAAT ACCTGACGTT CCACAAAAAC AAGATGAAAC TGTGATGCTT
GTGAAAGAAA GTCTCACTGA GACTTCATTT GAGTCAATGA TAGAATATGA AAATAAGGAA
AAACTCAGTG CTTTGCCACC TGAGGGAGGA AAGCCATATT TGGAATCTTT TAAGCTCAGT
TTAGATAACA CAAAAGATAC CCTGTTACCT GATGAAGTTT CAACATTGAG CAAAAAGGAG
AAAATTCCTT TGCAGATGGA GGAGCTCAGT ACTGCAGTTT ATTCAAATGA TGACTTATTT
ATTTCTAAGG AAGCACAGAT AAGAGAAACT GAAACGTTTT CAGATTCATC TCCAATTGAA
ATTATAGATG AGTTCCCTAC ATTGATCAGT TCTAAAACTG ATTCATTTTC TAAATTAGCC
AGGGAATATA CTGACCTAGA AGTATCCCAC AAAAGTGAAA TTGCTAATGC CCCGGATGGA
GCTGGGTCAT TGCCTTGCAC AGAATTGCCC CATGACCTTT CTTTGAAGAA CATACAACCC
AAAGTTGAAG AGAAAATCAG TTTCTCAGAT GACTTTTCTA AAAATGGGTC TGCTACATCA
AAGGTGCTCT TATTGCCTCC AGATGTTTCT GCTTTGGCCA CTCAAGCAGA GATAGAGAGC
ATAGTTAAAC CCAAAGTTCT TGTGAAAGAA GCTGAGAAAA AACTTCCTTC CGATACAGAA
AAAGAGGACA GATCACCATC TGCTATATTT TCAGCAGAGC TGAGTAAAAC TTCAGTTGTT
GACCTCCTGT ACTGGAGAGA CATTAAGAAG ACTGGAGTGG TGTTTGGTGC CAGCCTATTC
CTGCTGCTTT CATTGACAGT ATTCAGCATT GTGAGCGTAA CAGCCTACAT TGCCTTGGCC
CTGCTCTCTG TGACCATCAG CTTTAGGATA TACAAGGGTG TGATCCAAGC TATCCAGAAA
TCAGATGAAG GCCACCCATT CAGGGCATAT CTGGAATCTG AAGTTGCTAT ATCTGAGGAG
TTGGTTCAGA AGTACAGTAA TTCTGCTCTT GGTCATGTGA ACTGCACGAT AAAGGAACTC
AGGCGCCTCT TCTTAGTTGA TGATTTAGTT GATTCTCTGA AGTTTGCAGT GTTGATGTGG
GTATTTACCT ATGTTGGTGC CTTGTTTAAT GGTCTGACAC TACTGATTTT GGCTCTCATT
TCACTCTTCA GTGTTCCTGT TATTTATGAA CGGCATCAGG CACAGATAGA TCATTATCTA
GGACTTGCAA ATAAGAATGT TAAAGATGCT ATGGCTAAAA TCCAAGCAAA AATCCCTGGA
TTGAAGCGCA AAGCTGAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu19329
Clone ID Related Accession (Same CDS sequence) NM_020532
Accession Version NM_020532.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product reticulon-4 isoform A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY123246.1, BC016165.1, AY123250.1, AF125103.1, AF132048.1 and AI671568.1. This sequence is a reference standard in the RefSeqGene project. On May 19, 2004 this sequence version replaced gi:28557782. Transcript Variant: This variant (1), also known as Nogo-A and RTN-XL, represents the longest transcript, and encodes the longest isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150182.1, AB040462.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGAAGACC TGGACCAGTC TCCTCTGGTC TCGTCCTCGG ACAGCCCACC CCGGCCGCAG 
CCCGCGTTCA AGTACCAGTT CGTGAGGGAG CCCGAGGACG AGGAGGAAGA AGAGGAGGAG
GAAGAGGAGG ACGAGGACGA AGACCTGGAG GAGCTGGAGG TGCTGGAGAG GAAGCCCGCC
GCCGGGCTGT CCGCGGCCCC AGTGCCCACC GCCCCTGCCG CCGGCGCGCC CCTGATGGAC
TTCGGAAATG ACTTCGTGCC GCCGGCGCCC CGGGGACCCC TGCCGGCCGC TCCCCCCGTC
GCCCCGGAGC GGCAGCCGTC TTGGGACCCG AGCCCGGTGT CGTCGACCGT GCCCGCGCCA
TCCCCGCTGT CTGCTGCCGC AGTCTCGCCC TCCAAGCTCC CTGAGGACGA CGAGCCTCCG
GCCCGGCCTC CCCCTCCTCC CCCGGCCAGC GTGAGCCCCC AGGCAGAGCC CGTGTGGACC
CCGCCAGCCC CGGCTCCCGC CGCGCCCCCC TCCACCCCGG CCGCGCCCAA GCGCAGGGGC
TCCTCGGGCT CAGTGGATGA GACCCTTTTT GCTCTTCCTG CTGCATCTGA GCCTGTGATA
CGCTCCTCTG CAGAAAATAT GGACTTGAAG GAGCAGCCAG GTAACACTAT TTCGGCTGGT
CAAGAGGATT TCCCATCTGT CCTGCTTGAA ACTGCTGCTT CTCTTCCTTC TCTGTCTCCT
CTCTCAGCCG CTTCTTTCAA AGAACATGAA TACCTTGGTA ATTTGTCAAC AGTATTACCC
ACTGAAGGAA CACTTCAAGA AAATGTCAGT GAAGCTTCTA AAGAGGTCTC AGAGAAGGCA
AAAACTCTAC TCATAGATAG AGATTTAACA GAGTTTTCAG AATTAGAATA CTCAGAAATG
GGATCATCGT TCAGTGTCTC TCCAAAAGCA GAATCTGCCG TAATAGTAGC AAATCCTAGG
GAAGAAATAA TCGTGAAAAA TAAAGATGAA GAAGAGAAGT TAGTTAGTAA TAACATCCTT
CATAATCAAC AAGAGTTACC TACAGCTCTT ACTAAATTGG TTAAAGAGGA TGAAGTTGTG
TCTTCAGAAA AAGCAAAAGA CAGTTTTAAT GAAAAGAGAG TTGCAGTGGA AGCTCCTATG
AGGGAGGAAT ATGCAGACTT CAAACCATTT GAGCGAGTAT GGGAAGTGAA AGATAGTAAG
GAAGATAGTG ATATGTTGGC TGCTGGAGGT AAAATCGAGA GCAACTTGGA AAGTAAAGTG
GATAAAAAAT GTTTTGCAGA TAGCCTTGAG CAAACTAATC ACGAAAAAGA TAGTGAGAGT
AGTAATGATG ATACTTCTTT CCCCAGTACG CCAGAAGGTA TAAAGGATCG TTCAGGAGCA
TATATCACAT GTGCTCCCTT TAACCCAGCA GCAACTGAGA GCATTGCAAC AAACATTTTT
CCTTTGTTAG GAGATCCTAC TTCAGAAAAT AAGACCGATG AAAAAAAAAT AGAAGAAAAG
AAGGCCCAAA TAGTAACAGA GAAGAATACT AGCACCAAAA CATCAAACCC TTTTCTTGTA
GCAGCACAGG ATTCTGAGAC AGATTATGTC ACAACAGATA ATTTAACAAA GGTGACTGAG
GAAGTCGTGG CAAACATGCC TGAAGGCCTG ACTCCAGATT TAGTACAGGA AGCATGTGAA
AGTGAATTGA ATGAAGTTAC TGGTACAAAG ATTGCTTATG AAACAAAAAT GGACTTGGTT
CAAACATCAG AAGTTATGCA AGAGTCACTC TATCCTGCAG CACAGCTTTG CCCATCATTT
GAAGAGTCAG AAGCTACTCC TTCACCAGTT TTGCCTGACA TTGTTATGGA AGCACCATTG
AATTCTGCAG TTCCTAGTGC TGGTGCTTCC GTGATACAGC CCAGCTCATC ACCATTAGAA
GCTTCTTCAG TTAATTATGA AAGCATAAAA CATGAGCCTG AAAACCCCCC ACCATATGAA
GAGGCCATGA GTGTATCACT AAAAAAAGTA TCAGGAATAA AGGAAGAAAT TAAAGAGCCT
GAAAATATTA ATGCAGCTCT TCAAGAAACA GAAGCTCCTT ATATATCTAT TGCATGTGAT
TTAATTAAAG AAACAAAGCT TTCTGCTGAA CCAGCTCCGG ATTTCTCTGA TTATTCAGAA
ATGGCAAAAG TTGAACAGCC AGTGCCTGAT CATTCTGAGC TAGTTGAAGA TTCCTCACCT
GATTCTGAAC CAGTTGACTT ATTTAGTGAT GATTCAATAC CTGACGTTCC ACAAAAACAA
GATGAAACTG TGATGCTTGT GAAAGAAAGT CTCACTGAGA CTTCATTTGA GTCAATGATA
GAATATGAAA ATAAGGAAAA ACTCAGTGCT TTGCCACCTG AGGGAGGAAA GCCATATTTG
GAATCTTTTA AGCTCAGTTT AGATAACACA AAAGATACCC TGTTACCTGA TGAAGTTTCA
ACATTGAGCA AAAAGGAGAA AATTCCTTTG CAGATGGAGG AGCTCAGTAC TGCAGTTTAT
TCAAATGATG ACTTATTTAT TTCTAAGGAA GCACAGATAA GAGAAACTGA AACGTTTTCA
GATTCATCTC CAATTGAAAT TATAGATGAG TTCCCTACAT TGATCAGTTC TAAAACTGAT
TCATTTTCTA AATTAGCCAG GGAATATACT GACCTAGAAG TATCCCACAA AAGTGAAATT
GCTAATGCCC CGGATGGAGC TGGGTCATTG CCTTGCACAG AATTGCCCCA TGACCTTTCT
TTGAAGAACA TACAACCCAA AGTTGAAGAG AAAATCAGTT TCTCAGATGA CTTTTCTAAA
AATGGGTCTG CTACATCAAA GGTGCTCTTA TTGCCTCCAG ATGTTTCTGC TTTGGCCACT
CAAGCAGAGA TAGAGAGCAT AGTTAAACCC AAAGTTCTTG TGAAAGAAGC TGAGAAAAAA
CTTCCTTCCG ATACAGAAAA AGAGGACAGA TCACCATCTG CTATATTTTC AGCAGAGCTG
AGTAAAACTT CAGTTGTTGA CCTCCTGTAC TGGAGAGACA TTAAGAAGAC TGGAGTGGTG
TTTGGTGCCA GCCTATTCCT GCTGCTTTCA TTGACAGTAT TCAGCATTGT GAGCGTAACA
GCCTACATTG CCTTGGCCCT GCTCTCTGTG ACCATCAGCT TTAGGATATA CAAGGGTGTG
ATCCAAGCTA TCCAGAAATC AGATGAAGGC CACCCATTCA GGGCATATCT GGAATCTGAA
GTTGCTATAT CTGAGGAGTT GGTTCAGAAG TACAGTAATT CTGCTCTTGG TCATGTGAAC
TGCACGATAA AGGAACTCAG GCGCCTCTTC TTAGTTGATG ATTTAGTTGA TTCTCTGAAG
TTTGCAGTGT TGATGTGGGT ATTTACCTAT GTTGGTGCCT TGTTTAATGG TCTGACACTA
CTGATTTTGG CTCTCATTTC ACTCTTCAGT GTTCCTGTTA TTTATGAACG GCATCAGGCA
CAGATAGATC ATTATCTAGG ACTTGCAAAT AAGAATGTTA AAGATGCTAT GGCTAAAATC
CAAGCAAAAA TCCCTGGATT GAAGCGCAAA GCTGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_065393.1
CDS299..3877
Misc Feature(1)173..175(+)
Misc Feature(2)299..301(+)
Misc Feature(3)317..319(+)
Misc Feature(4)317..319(+)
Misc Feature(5)329..331(+)
Misc Feature(6)335..337(+)
Misc Feature(7)341..343(+)
Misc Feature(8)341..343(+)
Misc Feature(9)617..619(+)
Misc Feature(10)617..619(+)
Misc Feature(11)839..841(+)
Misc Feature(12)839..841(+)
Misc Feature(13)842..844(+)
Misc Feature(14)848..850(+)
Misc Feature(15)860..862(+)
Misc Feature(16)884..886(+)
Misc Feature(17)2876..2878(+)
Misc Feature(18)2885..2887(+)
Misc Feature(19)3311..3823(+)
Misc Feature(20)3353..3415(+)
Misc Feature(21)3608..3610(+)
Misc Feature(22)3698..3760(+)
Exon (1)1..854
Gene:RTN4
Gene Synonym:
Exon (2)855..911
Gene:RTN4
Gene Synonym:
Exon (3)912..3311
Gene:RTN4
Gene Synonym:
Exon (4)3312..3519
Gene:RTN4
Gene Synonym:
Exon (5)3520..3658
Gene:RTN4
Gene Synonym:
Exon (6)3659..3728
Gene:RTN4
Gene Synonym:
Exon (7)3729..3775
Gene:RTN4
Gene Synonym:
Exon (8)3776..3834
Gene:RTN4
Gene Synonym:
Exon (9)3835..4844
Gene:RTN4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_020532
Organism Homo sapiens (human)
Definition Homo sapiens reticulon 4 (RTN4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_020532

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
ATGGAAGACC TGGACCAGTC TCCTCTGGTC TCGTCCTCGG ACAGCCCACC CCGGCCGCAG 
CCCGCGTTCA AGTACCAGTT CGTGAGGGAG CCCGAGGACG AGGAGGAAGA AGAGGAGGAG
GAAGAGGAGG ACGAGGACGA AGACCTGGAG GAGCTGGAGG TGCTGGAGAG GAAGCCCGCC
GCCGGGCTGT CCGCGGCCCC AGTGCCCACC GCCCCTGCCG CCGGCGCGCC CCTGATGGAC
TTCGGAAATG ACTTCGTGCC GCCGGCGCCC CGGGGACCCC TGCCGGCCGC TCCCCCCGTC
GCCCCGGAGC GGCAGCCGTC TTGGGACCCG AGCCCGGTGT CGTCGACCGT GCCCGCGCCA
TCCCCGCTGT CTGCTGCCGC AGTCTCGCCC TCCAAGCTCC CTGAGGACGA CGAGCCTCCG
GCCCGGCCTC CCCCTCCTCC CCCGGCCAGC GTGAGCCCCC AGGCAGAGCC CGTGTGGACC
CCGCCAGCCC CGGCTCCCGC CGCGCCCCCC TCCACCCCGG CCGCGCCCAA GCGCAGGGGC
TCCTCGGGCT CAGTGGATGA GACCCTTTTT GCTCTTCCTG CTGCATCTGA GCCTGTGATA
CGCTCCTCTG CAGAAAATAT GGACTTGAAG GAGCAGCCAG GTAACACTAT TTCGGCTGGT
CAAGAGGATT TCCCATCTGT CCTGCTTGAA ACTGCTGCTT CTCTTCCTTC TCTGTCTCCT
CTCTCAGCCG CTTCTTTCAA AGAACATGAA TACCTTGGTA ATTTGTCAAC AGTATTACCC
ACTGAAGGAA CACTTCAAGA AAATGTCAGT GAAGCTTCTA AAGAGGTCTC AGAGAAGGCA
AAAACTCTAC TCATAGATAG AGATTTAACA GAGTTTTCAG AATTAGAATA CTCAGAAATG
GGATCATCGT TCAGTGTCTC TCCAAAAGCA GAATCTGCCG TAATAGTAGC AAATCCTAGG
GAAGAAATAA TCGTGAAAAA TAAAGATGAA GAAGAGAAGT TAGTTAGTAA TAACATCCTT
CATAATCAAC AAGAGTTACC TACAGCTCTT ACTAAATTGG TTAAAGAGGA TGAAGTTGTG
TCTTCAGAAA AAGCAAAAGA CAGTTTTAAT GAAAAGAGAG TTGCAGTGGA AGCTCCTATG
AGGGAGGAAT ATGCAGACTT CAAACCATTT GAGCGAGTAT GGGAAGTGAA AGATAGTAAG
GAAGATAGTG ATATGTTGGC TGCTGGAGGT AAAATCGAGA GCAACTTGGA AAGTAAAGTG
GATAAAAAAT GTTTTGCAGA TAGCCTTGAG CAAACTAATC ACGAAAAAGA TAGTGAGAGT
AGTAATGATG ATACTTCTTT CCCCAGTACG CCAGAAGGTA TAAAGGATCG TTCAGGAGCA
TATATCACAT GTGCTCCCTT TAACCCAGCA GCAACTGAGA GCATTGCAAC AAACATTTTT
CCTTTGTTAG GAGATCCTAC TTCAGAAAAT AAGACCGATG AAAAAAAAAT AGAAGAAAAG
AAGGCCCAAA TAGTAACAGA GAAGAATACT AGCACCAAAA CATCAAACCC TTTTCTTGTA
GCAGCACAGG ATTCTGAGAC AGATTATGTC ACAACAGATA ATTTAACAAA GGTGACTGAG
GAAGTCGTGG CAAACATGCC TGAAGGCCTG ACTCCAGATT TAGTACAGGA AGCATGTGAA
AGTGAATTGA ATGAAGTTAC TGGTACAAAG ATTGCTTATG AAACAAAAAT GGACTTGGTT
CAAACATCAG AAGTTATGCA AGAGTCACTC TATCCTGCAG CACAGCTTTG CCCATCATTT
GAAGAGTCAG AAGCTACTCC TTCACCAGTT TTGCCTGACA TTGTTATGGA AGCACCATTG
AATTCTGCAG TTCCTAGTGC TGGTGCTTCC GTGATACAGC CCAGCTCATC ACCATTAGAA
GCTTCTTCAG TTAATTATGA AAGCATAAAA CATGAGCCTG AAAACCCCCC ACCATATGAA
GAGGCCATGA GTGTATCACT AAAAAAAGTA TCAGGAATAA AGGAAGAAAT TAAAGAGCCT
GAAAATATTA ATGCAGCTCT TCAAGAAACA GAAGCTCCTT ATATATCTAT TGCATGTGAT
TTAATTAAAG AAACAAAGCT TTCTGCTGAA CCAGCTCCGG ATTTCTCTGA TTATTCAGAA
ATGGCAAAAG TTGAACAGCC AGTGCCTGAT CATTCTGAGC TAGTTGAAGA TTCCTCACCT
GATTCTGAAC CAGTTGACTT ATTTAGTGAT GATTCAATAC CTGACGTTCC ACAAAAACAA
GATGAAACTG TGATGCTTGT GAAAGAAAGT CTCACTGAGA CTTCATTTGA GTCAATGATA
GAATATGAAA ATAAGGAAAA ACTCAGTGCT TTGCCACCTG AGGGAGGAAA GCCATATTTG
GAATCTTTTA AGCTCAGTTT AGATAACACA AAAGATACCC TGTTACCTGA TGAAGTTTCA
ACATTGAGCA AAAAGGAGAA AATTCCTTTG CAGATGGAGG AGCTCAGTAC TGCAGTTTAT
TCAAATGATG ACTTATTTAT TTCTAAGGAA GCACAGATAA GAGAAACTGA AACGTTTTCA
GATTCATCTC CAATTGAAAT TATAGATGAG TTCCCTACAT TGATCAGTTC TAAAACTGAT
TCATTTTCTA AATTAGCCAG GGAATATACT GACCTAGAAG TATCCCACAA AAGTGAAATT
GCTAATGCCC CGGATGGAGC TGGGTCATTG CCTTGCACAG AATTGCCCCA TGACCTTTCT
TTGAAGAACA TACAACCCAA AGTTGAAGAG AAAATCAGTT TCTCAGATGA CTTTTCTAAA
AATGGGTCTG CTACATCAAA GGTGCTCTTA TTGCCTCCAG ATGTTTCTGC TTTGGCCACT
CAAGCAGAGA TAGAGAGCAT AGTTAAACCC AAAGTTCTTG TGAAAGAAGC TGAGAAAAAA
CTTCCTTCCG ATACAGAAAA AGAGGACAGA TCACCATCTG CTATATTTTC AGCAGAGCTG
AGTAAAACTT CAGTTGTTGA CCTCCTGTAC TGGAGAGACA TTAAGAAGAC TGGAGTGGTG
TTTGGTGCCA GCCTATTCCT GCTGCTTTCA TTGACAGTAT TCAGCATTGT GAGCGTAACA
GCCTACATTG CCTTGGCCCT GCTCTCTGTG ACCATCAGCT TTAGGATATA CAAGGGTGTG
ATCCAAGCTA TCCAGAAATC AGATGAAGGC CACCCATTCA GGGCATATCT GGAATCTGAA
GTTGCTATAT CTGAGGAGTT GGTTCAGAAG TACAGTAATT CTGCTCTTGG TCATGTGAAC
TGCACGATAA AGGAACTCAG GCGCCTCTTC TTAGTTGATG ATTTAGTTGA TTCTCTGAAG
TTTGCAGTGT TGATGTGGGT ATTTACCTAT GTTGGTGCCT TGTTTAATGG TCTGACACTA
CTGATTTTGG CTCTCATTTC ACTCTTCAGT GTTCCTGTTA TTTATGAACG GCATCAGGCA
CAGATAGATC ATTATCTAGG ACTTGCAAAT AAGAATGTTA AAGATGCTAT GGCTAAAATC
CAAGCAAAAA TCCCTGGATT GAAGCGCAAA GCTGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15978
Clone ID Related Accession (Same CDS sequence) NM_207520
Accession Version NM_207520.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1179bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product reticulon-4 isoform D
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY123246.1, BC016165.1, AY123250.1, AF125103.1, AF132048.1 and AI671568.1. Transcript Variant: This variant (4), also known as Foocen-m2 and RTN4-B2, lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (D), that is missing an internal segment, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY102278.1, BC016165.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAAGACC TGGACCAGTC TCCTCTGGTC TCGTCCTCGG ACAGCCCACC CCGGCCGCAG 
CCCGCGTTCA AGTACCAGTT CGTGAGGGAG CCCGAGGACG AGGAGGAAGA AGAGGAGGAG
GAAGAGGAGG ACGAGGACGA AGACCTGGAG GAGCTGGAGG TGCTGGAGAG GAAGCCCGCC
GCCGGGCTGT CCGCGGCCCC AGTGCCCACC GCCCCTGCCG CCGGCGCGCC CCTGATGGAC
TTCGGAAATG ACTTCGTGCC GCCGGCGCCC CGGGGACCCC TGCCGGCCGC TCCCCCCGTC
GCCCCGGAGC GGCAGCCGTC TTGGGACCCG AGCCCGGTGT CGTCGACCGT GCCCGCGCCA
TCCCCGCTGT CTGCTGCCGC AGTCTCGCCC TCCAAGCTCC CTGAGGACGA CGAGCCTCCG
GCCCGGCCTC CCCCTCCTCC CCCGGCCAGC GTGAGCCCCC AGGCAGAGCC CGTGTGGACC
CCGCCAGCCC CGGCTCCCGC CGCGCCCCCC TCCACCCCGG CCGCGCCCAA GCGCAGGGGC
TCCTCGGGCT CAGTGGATGA GACCCTTTTT GCTCTTCCTG CTGCATCTGA GCCTGTGATA
CGCTCCTCTG CAGTTGTTGA CCTCCTGTAC TGGAGAGACA TTAAGAAGAC TGGAGTGGTG
TTTGGTGCCA GCCTATTCCT GCTGCTTTCA TTGACAGTAT TCAGCATTGT GAGCGTAACA
GCCTACATTG CCTTGGCCCT GCTCTCTGTG ACCATCAGCT TTAGGATATA CAAGGGTGTG
ATCCAAGCTA TCCAGAAATC AGATGAAGGC CACCCATTCA GGGCATATCT GGAATCTGAA
GTTGCTATAT CTGAGGAGTT GGTTCAGAAG TACAGTAATT CTGCTCTTGG TCATGTGAAC
TGCACGATAA AGGAACTCAG GCGCCTCTTC TTAGTTGATG ATTTAGTTGA TTCTCTGAAG
TTTGCAGTGT TGATGTGGGT ATTTACCTAT GTTGGTGCCT TGTTTAATGG TCTGACACTA
CTGATTTTGG CTCTCATTTC ACTCTTCAGT GTTCCTGTTA TTTATGAACG GCATCAGGCA
CAGATAGATC ATTATCTAGG ACTTGCAAAT AAGAATGTTA AAGATGCTAT GGCTAAAATC
CAAGCAAAAA TCCCTGGATT GAAGCGCAAA GCTGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_997403.1
CDS299..1477
Misc Feature(1)173..175(+)
Misc Feature(2)299..301(+)
Misc Feature(3)317..319(+)
Misc Feature(4)317..319(+)
Misc Feature(5)329..331(+)
Misc Feature(6)335..337(+)
Misc Feature(7)341..343(+)
Misc Feature(8)341..343(+)
Misc Feature(9)617..619(+)
Misc Feature(10)617..619(+)
Misc Feature(11)839..841(+)
Misc Feature(12)839..841(+)
Misc Feature(13)842..844(+)
Misc Feature(14)848..850(+)
Misc Feature(15)860..862(+)
Misc Feature(16)884..886(+)
Misc Feature(17)911..1423(+)
Exon (1)1..854
Gene:RTN4
Gene Synonym:
Exon (2)855..911
Gene:RTN4
Gene Synonym:
Exon (3)912..1119
Gene:RTN4
Gene Synonym:
Exon (4)1120..1258
Gene:RTN4
Gene Synonym:
Exon (5)1259..1328
Gene:RTN4
Gene Synonym:
Exon (6)1329..1375
Gene:RTN4
Gene Synonym:
Exon (7)1376..1434
Gene:RTN4
Gene Synonym:
Exon (8)1435..2444
Gene:RTN4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_207520
Organism Homo sapiens (human)
Definition Homo sapiens reticulon 4 (RTN4), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207520

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGGAAGACC TGGACCAGTC TCCTCTGGTC TCGTCCTCGG ACAGCCCACC CCGGCCGCAG 
CCCGCGTTCA AGTACCAGTT CGTGAGGGAG CCCGAGGACG AGGAGGAAGA AGAGGAGGAG
GAAGAGGAGG ACGAGGACGA AGACCTGGAG GAGCTGGAGG TGCTGGAGAG GAAGCCCGCC
GCCGGGCTGT CCGCGGCCCC AGTGCCCACC GCCCCTGCCG CCGGCGCGCC CCTGATGGAC
TTCGGAAATG ACTTCGTGCC GCCGGCGCCC CGGGGACCCC TGCCGGCCGC TCCCCCCGTC
GCCCCGGAGC GGCAGCCGTC TTGGGACCCG AGCCCGGTGT CGTCGACCGT GCCCGCGCCA
TCCCCGCTGT CTGCTGCCGC AGTCTCGCCC TCCAAGCTCC CTGAGGACGA CGAGCCTCCG
GCCCGGCCTC CCCCTCCTCC CCCGGCCAGC GTGAGCCCCC AGGCAGAGCC CGTGTGGACC
CCGCCAGCCC CGGCTCCCGC CGCGCCCCCC TCCACCCCGG CCGCGCCCAA GCGCAGGGGC
TCCTCGGGCT CAGTGGATGA GACCCTTTTT GCTCTTCCTG CTGCATCTGA GCCTGTGATA
CGCTCCTCTG CAGTTGTTGA CCTCCTGTAC TGGAGAGACA TTAAGAAGAC TGGAGTGGTG
TTTGGTGCCA GCCTATTCCT GCTGCTTTCA TTGACAGTAT TCAGCATTGT GAGCGTAACA
GCCTACATTG CCTTGGCCCT GCTCTCTGTG ACCATCAGCT TTAGGATATA CAAGGGTGTG
ATCCAAGCTA TCCAGAAATC AGATGAAGGC CACCCATTCA GGGCATATCT GGAATCTGAA
GTTGCTATAT CTGAGGAGTT GGTTCAGAAG TACAGTAATT CTGCTCTTGG TCATGTGAAC
TGCACGATAA AGGAACTCAG GCGCCTCTTC TTAGTTGATG ATTTAGTTGA TTCTCTGAAG
TTTGCAGTGT TGATGTGGGT ATTTACCTAT GTTGGTGCCT TGTTTAATGG TCTGACACTA
CTGATTTTGG CTCTCATTTC ACTCTTCAGT GTTCCTGTTA TTTATGAACG GCATCAGGCA
CAGATAGATC ATTATCTAGG ACTTGCAAAT AAGAATGTTA AAGATGCTAT GGCTAAAATC
CAAGCAAAAA TCCCTGGATT GAAGCGCAAA GCTGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15728
Clone ID Related Accession (Same CDS sequence) NM_153828
Accession Version NM_153828.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1122bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product reticulon-4 isoform B
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY123246.1, BC016165.1, AY123250.1, AF125103.1, AF132048.1 and AI671568.1. On May 19, 2004 this sequence version replaced gi:24431932. Transcript Variant: This variant (2), also known as Foocen-M, Nogo-B, RTN-XS and RTN4-B1, lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (B), that is missing an internal segment, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY102277.1, BC068991.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGACC TGGACCAGTC TCCTCTGGTC TCGTCCTCGG ACAGCCCACC CCGGCCGCAG 
CCCGCGTTCA AGTACCAGTT CGTGAGGGAG CCCGAGGACG AGGAGGAAGA AGAGGAGGAG
GAAGAGGAGG ACGAGGACGA AGACCTGGAG GAGCTGGAGG TGCTGGAGAG GAAGCCCGCC
GCCGGGCTGT CCGCGGCCCC AGTGCCCACC GCCCCTGCCG CCGGCGCGCC CCTGATGGAC
TTCGGAAATG ACTTCGTGCC GCCGGCGCCC CGGGGACCCC TGCCGGCCGC TCCCCCCGTC
GCCCCGGAGC GGCAGCCGTC TTGGGACCCG AGCCCGGTGT CGTCGACCGT GCCCGCGCCA
TCCCCGCTGT CTGCTGCCGC AGTCTCGCCC TCCAAGCTCC CTGAGGACGA CGAGCCTCCG
GCCCGGCCTC CCCCTCCTCC CCCGGCCAGC GTGAGCCCCC AGGCAGAGCC CGTGTGGACC
CCGCCAGCCC CGGCTCCCGC CGCGCCCCCC TCCACCCCGG CCGCGCCCAA GCGCAGGGGC
TCCTCGGGCT CAGTGGTTGT TGACCTCCTG TACTGGAGAG ACATTAAGAA GACTGGAGTG
GTGTTTGGTG CCAGCCTATT CCTGCTGCTT TCATTGACAG TATTCAGCAT TGTGAGCGTA
ACAGCCTACA TTGCCTTGGC CCTGCTCTCT GTGACCATCA GCTTTAGGAT ATACAAGGGT
GTGATCCAAG CTATCCAGAA ATCAGATGAA GGCCACCCAT TCAGGGCATA TCTGGAATCT
GAAGTTGCTA TATCTGAGGA GTTGGTTCAG AAGTACAGTA ATTCTGCTCT TGGTCATGTG
AACTGCACGA TAAAGGAACT CAGGCGCCTC TTCTTAGTTG ATGATTTAGT TGATTCTCTG
AAGTTTGCAG TGTTGATGTG GGTATTTACC TATGTTGGTG CCTTGTTTAA TGGTCTGACA
CTACTGATTT TGGCTCTCAT TTCACTCTTC AGTGTTCCTG TTATTTATGA ACGGCATCAG
GCACAGATAG ATCATTATCT AGGACTTGCA AATAAGAATG TTAAAGATGC TATGGCTAAA
ATCCAAGCAA AAATCCCTGG ATTGAAGCGC AAAGCTGAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_722550.1
CDS299..1420
Misc Feature(1)173..175(+)
Misc Feature(2)299..301(+)
Misc Feature(3)317..319(+)
Misc Feature(4)317..319(+)
Misc Feature(5)329..331(+)
Misc Feature(6)335..337(+)
Misc Feature(7)341..343(+)
Misc Feature(8)341..343(+)
Misc Feature(9)617..619(+)
Misc Feature(10)617..619(+)
Misc Feature(11)839..841(+)
Misc Feature(12)842..844(+)
Misc Feature(13)854..1366(+)
Exon (1)1..854
Gene:RTN4
Gene Synonym:
Exon (2)855..1062
Gene:RTN4
Gene Synonym:
Exon (3)1063..1201
Gene:RTN4
Gene Synonym:
Exon (4)1202..1271
Gene:RTN4
Gene Synonym:
Exon (5)1272..1318
Gene:RTN4
Gene Synonym:
Exon (6)1319..1377
Gene:RTN4
Gene Synonym:
Exon (7)1378..2387
Gene:RTN4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_153828
Organism Homo sapiens (human)
Definition Homo sapiens reticulon 4 (RTN4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153828

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGAAGACC TGGACCAGTC TCCTCTGGTC TCGTCCTCGG ACAGCCCACC CCGGCCGCAG 
CCCGCGTTCA AGTACCAGTT CGTGAGGGAG CCCGAGGACG AGGAGGAAGA AGAGGAGGAG
GAAGAGGAGG ACGAGGACGA AGACCTGGAG GAGCTGGAGG TGCTGGAGAG GAAGCCCGCC
GCCGGGCTGT CCGCGGCCCC AGTGCCCACC GCCCCTGCCG CCGGCGCGCC CCTGATGGAC
TTCGGAAATG ACTTCGTGCC GCCGGCGCCC CGGGGACCCC TGCCGGCCGC TCCCCCCGTC
GCCCCGGAGC GGCAGCCGTC TTGGGACCCG AGCCCGGTGT CGTCGACCGT GCCCGCGCCA
TCCCCGCTGT CTGCTGCCGC AGTCTCGCCC TCCAAGCTCC CTGAGGACGA CGAGCCTCCG
GCCCGGCCTC CCCCTCCTCC CCCGGCCAGC GTGAGCCCCC AGGCAGAGCC CGTGTGGACC
CCGCCAGCCC CGGCTCCCGC CGCGCCCCCC TCCACCCCGG CCGCGCCCAA GCGCAGGGGC
TCCTCGGGCT CAGTGGTTGT TGACCTCCTG TACTGGAGAG ACATTAAGAA GACTGGAGTG
GTGTTTGGTG CCAGCCTATT CCTGCTGCTT TCATTGACAG TATTCAGCAT TGTGAGCGTA
ACAGCCTACA TTGCCTTGGC CCTGCTCTCT GTGACCATCA GCTTTAGGAT ATACAAGGGT
GTGATCCAAG CTATCCAGAA ATCAGATGAA GGCCACCCAT TCAGGGCATA TCTGGAATCT
GAAGTTGCTA TATCTGAGGA GTTGGTTCAG AAGTACAGTA ATTCTGCTCT TGGTCATGTG
AACTGCACGA TAAAGGAACT CAGGCGCCTC TTCTTAGTTG ATGATTTAGT TGATTCTCTG
AAGTTTGCAG TGTTGATGTG GGTATTTACC TATGTTGGTG CCTTGTTTAA TGGTCTGACA
CTACTGATTT TGGCTCTCAT TTCACTCTTC AGTGTTCCTG TTATTTATGA ACGGCATCAG
GCACAGATAG ATCATTATCT AGGACTTGCA AATAAGAATG TTAAAGATGC TATGGCTAAA
ATCCAAGCAA AAATCCCTGG ATTGAAGCGC AAAGCTGAAT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15762
Clone ID Related Accession (Same CDS sequence) NM_007008
Accession Version NM_007008.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 600bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product reticulon-4 isoform C
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY102276.1, AF125103.1, AF132048.1 and AI671568.1. On May 19, 2004 this sequence version replaced gi:5902015. Transcript Variant: This variant (3), also known as Foocen-S and Nogo-C, differs in the 5' UTR and the 5' coding region, compared to variant 1. The resulting isoform (C) contains a distinct N-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF125103.1, BC014366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA962337, SAMEA962343 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
ATGGACGGTC AGAAGAAAAA TTGGAAGGAC AAGGTTGTTG ACCTCCTGTA CTGGAGAGAC 
ATTAAGAAGA CTGGAGTGGT GTTTGGTGCC AGCCTATTCC TGCTGCTTTC ATTGACAGTA
TTCAGCATTG TGAGCGTAAC AGCCTACATT GCCTTGGCCC TGCTCTCTGT GACCATCAGC
TTTAGGATAT ACAAGGGTGT GATCCAAGCT ATCCAGAAAT CAGATGAAGG CCACCCATTC
AGGGCATATC TGGAATCTGA AGTTGCTATA TCTGAGGAGT TGGTTCAGAA GTACAGTAAT
TCTGCTCTTG GTCATGTGAA CTGCACGATA AAGGAACTCA GGCGCCTCTT CTTAGTTGAT
GATTTAGTTG ATTCTCTGAA GTTTGCAGTG TTGATGTGGG TATTTACCTA TGTTGGTGCC
TTGTTTAATG GTCTGACACT ACTGATTTTG GCTCTCATTT CACTCTTCAG TGTTCCTGTT
ATTTATGAAC GGCATCAGGC ACAGATAGAT CATTATCTAG GACTTGCAAA TAAGAATGTT
AAAGATGCTA TGGCTAAAAT CCAAGCAAAA ATCCCTGGAT TGAAGCGCAA AGCTGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_008939.1
CDS215..814
Misc Feature(1)206..208(+)
Misc Feature(2)248..760(+)
Exon (1)1..248
Gene:RTN4
Gene Synonym:
Exon (2)249..456
Gene:RTN4
Gene Synonym:
Exon (3)457..595
Gene:RTN4
Gene Synonym:
Exon (4)596..665
Gene:RTN4
Gene Synonym:
Exon (5)666..712
Gene:RTN4
Gene Synonym:
Exon (6)713..771
Gene:RTN4
Gene Synonym:
Exon (7)772..1781
Gene:RTN4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_007008
Organism Homo sapiens (human)
Definition Homo sapiens reticulon 4 (RTN4), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_007008

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGACGGTC AGAAGAAAAA TTGGAAGGAC AAGGTTGTTG ACCTCCTGTA CTGGAGAGAC 
ATTAAGAAGA CTGGAGTGGT GTTTGGTGCC AGCCTATTCC TGCTGCTTTC ATTGACAGTA
TTCAGCATTG TGAGCGTAAC AGCCTACATT GCCTTGGCCC TGCTCTCTGT GACCATCAGC
TTTAGGATAT ACAAGGGTGT GATCCAAGCT ATCCAGAAAT CAGATGAAGG CCACCCATTC
AGGGCATATC TGGAATCTGA AGTTGCTATA TCTGAGGAGT TGGTTCAGAA GTACAGTAAT
TCTGCTCTTG GTCATGTGAA CTGCACGATA AAGGAACTCA GGCGCCTCTT CTTAGTTGAT
GATTTAGTTG ATTCTCTGAA GTTTGCAGTG TTGATGTGGG TATTTACCTA TGTTGGTGCC
TTGTTTAATG GTCTGACACT ACTGATTTTG GCTCTCATTT CACTCTTCAG TGTTCCTGTT
ATTTATGAAC GGCATCAGGC ACAGATAGAT CATTATCTAG GACTTGCAAA TAAGAATGTT
AAAGATGCTA TGGCTAAAAT CCAAGCAAAA ATCCCTGGAT TGAAGCGCAA AGCTGAATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15795
Clone ID Related Accession (Same CDS sequence) NM_207521 , XM_005264434 , XM_011532991 , XM_011532992
Accession Version NM_207521.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2961bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product reticulon-4 isoform E
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF333336.1, BC016165.1, AY123250.1, AF125103.1, AF132048.1 and AI671568.1. Transcript Variant: This variant (5), also known as hRTN4-E, differs in the 5' UTR and the 5' coding region, compared to variant 1. The resulting isoform (E) has a shorter N-terminus, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY123248.1, AF333336.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGACTTGA AGGAGCAGCC AGGTAACACT ATTTCGGCTG GTCAAGAGGA TTTCCCATCT 
GTCCTGCTTG AAACTGCTGC TTCTCTTCCT TCTCTGTCTC CTCTCTCAGC CGCTTCTTTC
AAAGAACATG AATACCTTGG TAATTTGTCA ACAGTATTAC CCACTGAAGG AACACTTCAA
GAAAATGTCA GTGAAGCTTC TAAAGAGGTC TCAGAGAAGG CAAAAACTCT ACTCATAGAT
AGAGATTTAA CAGAGTTTTC AGAATTAGAA TACTCAGAAA TGGGATCATC GTTCAGTGTC
TCTCCAAAAG CAGAATCTGC CGTAATAGTA GCAAATCCTA GGGAAGAAAT AATCGTGAAA
AATAAAGATG AAGAAGAGAA GTTAGTTAGT AATAACATCC TTCATAATCA ACAAGAGTTA
CCTACAGCTC TTACTAAATT GGTTAAAGAG GATGAAGTTG TGTCTTCAGA AAAAGCAAAA
GACAGTTTTA ATGAAAAGAG AGTTGCAGTG GAAGCTCCTA TGAGGGAGGA ATATGCAGAC
TTCAAACCAT TTGAGCGAGT ATGGGAAGTG AAAGATAGTA AGGAAGATAG TGATATGTTG
GCTGCTGGAG GTAAAATCGA GAGCAACTTG GAAAGTAAAG TGGATAAAAA ATGTTTTGCA
GATAGCCTTG AGCAAACTAA TCACGAAAAA GATAGTGAGA GTAGTAATGA TGATACTTCT
TTCCCCAGTA CGCCAGAAGG TATAAAGGAT CGTTCAGGAG CATATATCAC ATGTGCTCCC
TTTAACCCAG CAGCAACTGA GAGCATTGCA ACAAACATTT TTCCTTTGTT AGGAGATCCT
ACTTCAGAAA ATAAGACCGA TGAAAAAAAA ATAGAAGAAA AGAAGGCCCA AATAGTAACA
GAGAAGAATA CTAGCACCAA AACATCAAAC CCTTTTCTTG TAGCAGCACA GGATTCTGAG
ACAGATTATG TCACAACAGA TAATTTAACA AAGGTGACTG AGGAAGTCGT GGCAAACATG
CCTGAAGGCC TGACTCCAGA TTTAGTACAG GAAGCATGTG AAAGTGAATT GAATGAAGTT
ACTGGTACAA AGATTGCTTA TGAAACAAAA ATGGACTTGG TTCAAACATC AGAAGTTATG
CAAGAGTCAC TCTATCCTGC AGCACAGCTT TGCCCATCAT TTGAAGAGTC AGAAGCTACT
CCTTCACCAG TTTTGCCTGA CATTGTTATG GAAGCACCAT TGAATTCTGC AGTTCCTAGT
GCTGGTGCTT CCGTGATACA GCCCAGCTCA TCACCATTAG AAGCTTCTTC AGTTAATTAT
GAAAGCATAA AACATGAGCC TGAAAACCCC CCACCATATG AAGAGGCCAT GAGTGTATCA
CTAAAAAAAG TATCAGGAAT AAAGGAAGAA ATTAAAGAGC CTGAAAATAT TAATGCAGCT
CTTCAAGAAA CAGAAGCTCC TTATATATCT ATTGCATGTG ATTTAATTAA AGAAACAAAG
CTTTCTGCTG AACCAGCTCC GGATTTCTCT GATTATTCAG AAATGGCAAA AGTTGAACAG
CCAGTGCCTG ATCATTCTGA GCTAGTTGAA GATTCCTCAC CTGATTCTGA ACCAGTTGAC
TTATTTAGTG ATGATTCAAT ACCTGACGTT CCACAAAAAC AAGATGAAAC TGTGATGCTT
GTGAAAGAAA GTCTCACTGA GACTTCATTT GAGTCAATGA TAGAATATGA AAATAAGGAA
AAACTCAGTG CTTTGCCACC TGAGGGAGGA AAGCCATATT TGGAATCTTT TAAGCTCAGT
TTAGATAACA CAAAAGATAC CCTGTTACCT GATGAAGTTT CAACATTGAG CAAAAAGGAG
AAAATTCCTT TGCAGATGGA GGAGCTCAGT ACTGCAGTTT ATTCAAATGA TGACTTATTT
ATTTCTAAGG AAGCACAGAT AAGAGAAACT GAAACGTTTT CAGATTCATC TCCAATTGAA
ATTATAGATG AGTTCCCTAC ATTGATCAGT TCTAAAACTG ATTCATTTTC TAAATTAGCC
AGGGAATATA CTGACCTAGA AGTATCCCAC AAAAGTGAAA TTGCTAATGC CCCGGATGGA
GCTGGGTCAT TGCCTTGCAC AGAATTGCCC CATGACCTTT CTTTGAAGAA CATACAACCC
AAAGTTGAAG AGAAAATCAG TTTCTCAGAT GACTTTTCTA AAAATGGGTC TGCTACATCA
AAGGTGCTCT TATTGCCTCC AGATGTTTCT GCTTTGGCCA CTCAAGCAGA GATAGAGAGC
ATAGTTAAAC CCAAAGTTCT TGTGAAAGAA GCTGAGAAAA AACTTCCTTC CGATACAGAA
AAAGAGGACA GATCACCATC TGCTATATTT TCAGCAGAGC TGAGTAAAAC TTCAGTTGTT
GACCTCCTGT ACTGGAGAGA CATTAAGAAG ACTGGAGTGG TGTTTGGTGC CAGCCTATTC
CTGCTGCTTT CATTGACAGT ATTCAGCATT GTGAGCGTAA CAGCCTACAT TGCCTTGGCC
CTGCTCTCTG TGACCATCAG CTTTAGGATA TACAAGGGTG TGATCCAAGC TATCCAGAAA
TCAGATGAAG GCCACCCATT CAGGGCATAT CTGGAATCTG AAGTTGCTAT ATCTGAGGAG
TTGGTTCAGA AGTACAGTAA TTCTGCTCTT GGTCATGTGA ACTGCACGAT AAAGGAACTC
AGGCGCCTCT TCTTAGTTGA TGATTTAGTT GATTCTCTGA AGTTTGCAGT GTTGATGTGG
GTATTTACCT ATGTTGGTGC CTTGTTTAAT GGTCTGACAC TACTGATTTT GGCTCTCATT
TCACTCTTCA GTGTTCCTGT TATTTATGAA CGGCATCAGG CACAGATAGA TCATTATCTA
GGACTTGCAA ATAAGAATGT TAAAGATGCT ATGGCTAAAA TCCAAGCAAA AATCCCTGGA
TTGAAGCGCA AAGCTGAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_997404.1
CDS175..3135
Misc Feature(1)40..42(+)
Misc Feature(2)2134..2136(+)
Misc Feature(3)2143..2145(+)
Misc Feature(4)2569..3081(+)
Exon (1)1..112
Gene:RTN4
Gene Synonym:
Exon (2)113..169
Gene:RTN4
Gene Synonym:
Exon (3)170..2569
Gene:RTN4
Gene Synonym:
Exon (4)2570..2777
Gene:RTN4
Gene Synonym:
Exon (5)2778..2916
Gene:RTN4
Gene Synonym:
Exon (6)2917..2986
Gene:RTN4
Gene Synonym:
Exon (7)2987..3033
Gene:RTN4
Gene Synonym:
Exon (8)3034..3092
Gene:RTN4
Gene Synonym:
Exon (9)3093..4102
Gene:RTN4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_207521
Organism Homo sapiens (human)
Definition Homo sapiens reticulon 4 (RTN4), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_207521

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
ATGGACTTGA AGGAGCAGCC AGGTAACACT ATTTCGGCTG GTCAAGAGGA TTTCCCATCT 
GTCCTGCTTG AAACTGCTGC TTCTCTTCCT TCTCTGTCTC CTCTCTCAGC CGCTTCTTTC
AAAGAACATG AATACCTTGG TAATTTGTCA ACAGTATTAC CCACTGAAGG AACACTTCAA
GAAAATGTCA GTGAAGCTTC TAAAGAGGTC TCAGAGAAGG CAAAAACTCT ACTCATAGAT
AGAGATTTAA CAGAGTTTTC AGAATTAGAA TACTCAGAAA TGGGATCATC GTTCAGTGTC
TCTCCAAAAG CAGAATCTGC CGTAATAGTA GCAAATCCTA GGGAAGAAAT AATCGTGAAA
AATAAAGATG AAGAAGAGAA GTTAGTTAGT AATAACATCC TTCATAATCA ACAAGAGTTA
CCTACAGCTC TTACTAAATT GGTTAAAGAG GATGAAGTTG TGTCTTCAGA AAAAGCAAAA
GACAGTTTTA ATGAAAAGAG AGTTGCAGTG GAAGCTCCTA TGAGGGAGGA ATATGCAGAC
TTCAAACCAT TTGAGCGAGT ATGGGAAGTG AAAGATAGTA AGGAAGATAG TGATATGTTG
GCTGCTGGAG GTAAAATCGA GAGCAACTTG GAAAGTAAAG TGGATAAAAA ATGTTTTGCA
GATAGCCTTG AGCAAACTAA TCACGAAAAA GATAGTGAGA GTAGTAATGA TGATACTTCT
TTCCCCAGTA CGCCAGAAGG TATAAAGGAT CGTTCAGGAG CATATATCAC ATGTGCTCCC
TTTAACCCAG CAGCAACTGA GAGCATTGCA ACAAACATTT TTCCTTTGTT AGGAGATCCT
ACTTCAGAAA ATAAGACCGA TGAAAAAAAA ATAGAAGAAA AGAAGGCCCA AATAGTAACA
GAGAAGAATA CTAGCACCAA AACATCAAAC CCTTTTCTTG TAGCAGCACA GGATTCTGAG
ACAGATTATG TCACAACAGA TAATTTAACA AAGGTGACTG AGGAAGTCGT GGCAAACATG
CCTGAAGGCC TGACTCCAGA TTTAGTACAG GAAGCATGTG AAAGTGAATT GAATGAAGTT
ACTGGTACAA AGATTGCTTA TGAAACAAAA ATGGACTTGG TTCAAACATC AGAAGTTATG
CAAGAGTCAC TCTATCCTGC AGCACAGCTT TGCCCATCAT TTGAAGAGTC AGAAGCTACT
CCTTCACCAG TTTTGCCTGA CATTGTTATG GAAGCACCAT TGAATTCTGC AGTTCCTAGT
GCTGGTGCTT CCGTGATACA GCCCAGCTCA TCACCATTAG AAGCTTCTTC AGTTAATTAT
GAAAGCATAA AACATGAGCC TGAAAACCCC CCACCATATG AAGAGGCCAT GAGTGTATCA
CTAAAAAAAG TATCAGGAAT AAAGGAAGAA ATTAAAGAGC CTGAAAATAT TAATGCAGCT
CTTCAAGAAA CAGAAGCTCC TTATATATCT ATTGCATGTG ATTTAATTAA AGAAACAAAG
CTTTCTGCTG AACCAGCTCC GGATTTCTCT GATTATTCAG AAATGGCAAA AGTTGAACAG
CCAGTGCCTG ATCATTCTGA GCTAGTTGAA GATTCCTCAC CTGATTCTGA ACCAGTTGAC
TTATTTAGTG ATGATTCAAT ACCTGACGTT CCACAAAAAC AAGATGAAAC TGTGATGCTT
GTGAAAGAAA GTCTCACTGA GACTTCATTT GAGTCAATGA TAGAATATGA AAATAAGGAA
AAACTCAGTG CTTTGCCACC TGAGGGAGGA AAGCCATATT TGGAATCTTT TAAGCTCAGT
TTAGATAACA CAAAAGATAC CCTGTTACCT GATGAAGTTT CAACATTGAG CAAAAAGGAG
AAAATTCCTT TGCAGATGGA GGAGCTCAGT ACTGCAGTTT ATTCAAATGA TGACTTATTT
ATTTCTAAGG AAGCACAGAT AAGAGAAACT GAAACGTTTT CAGATTCATC TCCAATTGAA
ATTATAGATG AGTTCCCTAC ATTGATCAGT TCTAAAACTG ATTCATTTTC TAAATTAGCC
AGGGAATATA CTGACCTAGA AGTATCCCAC AAAAGTGAAA TTGCTAATGC CCCGGATGGA
GCTGGGTCAT TGCCTTGCAC AGAATTGCCC CATGACCTTT CTTTGAAGAA CATACAACCC
AAAGTTGAAG AGAAAATCAG TTTCTCAGAT GACTTTTCTA AAAATGGGTC TGCTACATCA
AAGGTGCTCT TATTGCCTCC AGATGTTTCT GCTTTGGCCA CTCAAGCAGA GATAGAGAGC
ATAGTTAAAC CCAAAGTTCT TGTGAAAGAA GCTGAGAAAA AACTTCCTTC CGATACAGAA
AAAGAGGACA GATCACCATC TGCTATATTT TCAGCAGAGC TGAGTAAAAC TTCAGTTGTT
GACCTCCTGT ACTGGAGAGA CATTAAGAAG ACTGGAGTGG TGTTTGGTGC CAGCCTATTC
CTGCTGCTTT CATTGACAGT ATTCAGCATT GTGAGCGTAA CAGCCTACAT TGCCTTGGCC
CTGCTCTCTG TGACCATCAG CTTTAGGATA TACAAGGGTG TGATCCAAGC TATCCAGAAA
TCAGATGAAG GCCACCCATT CAGGGCATAT CTGGAATCTG AAGTTGCTAT ATCTGAGGAG
TTGGTTCAGA AGTACAGTAA TTCTGCTCTT GGTCATGTGA ACTGCACGAT AAAGGAACTC
AGGCGCCTCT TCTTAGTTGA TGATTTAGTT GATTCTCTGA AGTTTGCAGT GTTGATGTGG
GTATTTACCT ATGTTGGTGC CTTGTTTAAT GGTCTGACAC TACTGATTTT GGCTCTCATT
TCACTCTTCA GTGTTCCTGT TATTTATGAA CGGCATCAGG CACAGATAGA TCATTATCTA
GGACTTGCAA ATAAGAATGT TAAAGATGCT ATGGCTAAAA TCCAAGCAAA AATCCCTGGA
TTGAAGCGCA AAGCTGAATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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