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CACHD1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol CACHD1
Entrez Gene ID 57685
Full Name cache domain containing 1
General protein information
Preferred Names

cache domain containing 1

Names

VWFA and cache domain-containing protein 1
cache domain-containing protein 1
von Willebrand factor type A and cache domain containing 1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

1

1p31.3

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011541862.1 XP_011540164.1 VWFA and cache domain-containing protein 1 isoform X1
NM_020925.3 NP_065976.3 VWFA and cache domain-containing protein 1 isoform 1 precursor
NM_001293274.1 NP_001280203.1 VWFA and cache domain-containing protein 1 isoform 2
NM_020925.2 NP_065976.2 VWFA and cache domain-containing protein 1 isoform 1
XM_011541863.1 XP_011540165.1 VWFA and cache domain-containing protein 1 isoform X2
NM_020925.4 NP_065976.3 VWFA and cache domain-containing protein 1 isoform 1 precursor

Pathways from BioSystems


Homologies


Homo sapiens (human) CACHD1 NP_065976.2
Rattus norvegicus (Norway rat) Cachd1 NP_001178687.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP009043 XP_319795.4
Canis lupus familiaris (dog) CACHD1 XP_536680.3
Gallus gallus (chicken) CACHD1 XP_422524.4
Danio rerio (zebrafish) cachd1 XP_001334829.3
Drosophila melanogaster (fruit fly) CG16868 NP_611469.1
CACHD1 XP_001089910.2
Mus musculus (house mouse) Cachd1 NP_932154.1
Pan troglodytes (chimpanzee) CACHD1 XP_003308221.2
Bos taurus (cattle) CACHD1 XP_002686374.2
Xenopus tropicalis (tropical clawed frog) cachd1 XP_002931660.2

Gene Ontology


Bibliography

Related articles in PubMed

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following CACHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu27879 NM_001293274.1
Latest version!		 Homo sapiens cache domain containing 1 (CACHD1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu106935 NM_020925.4
Latest version!		 Homo sapiens cache domain containing 1 (CACHD1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu27880 NM_020925.2
Latest version!		 Homo sapiens cache domain containing 1 (CACHD1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $860.30
$1229.00
OHu70844 XM_011541862.1
Latest version!		 Homo sapiens cache domain containing 1 (CACHD1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu70845 XM_011541863.1
Latest version!		 Homo sapiens cache domain containing 1 (CACHD1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu106935 NM_020925.3 Homo sapiens cache domain containing 1 (CACHD1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu27879
Clone ID Related Accession (Same CDS sequence) NM_001293274.1
Accession Version NM_001293274.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2937bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-13
Organism Homo sapiens(human)
Product VWFA and cache domain-containing protein 1 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355515.12, BX537603.1, AL590405.19, BC039301.1 and AK022571.1. On Jun 6, 2014 this sequence version replaced XM_006710793.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BX537603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2152719 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGTCCACCT TTGTTAGCAG CGTGAAGTCT TCAGACAGTC CTACCCAGCA CGCAGTGGGA 
TTCCAAAAGG CATTTCAGCT GATTCGAAGT ACAAACAATA ACACAAAGTT CCAAGCAAAT 
ACAGACATGG TCATCATTTA CCTGTCAGCT GGCATTACAT CAAAGGACTC TTCGGAAGAA 
GATAAAAAAG CGACTCTCCA AGTCATCAAT GAAGAAAATA GCTTTCTAAA CAACTCTGTA 
ATGATTCTCA CCTATGCCCT CATGAACGAT GGGGTGACTG GTTTGAAAGA GCTGGCTTTT 
CTGAGGGATC TAGCTGAACA GAATTCAGGG AAGTACGGTG TGCCAGACCG GATGGCCTTG 
CCTGTGATTA AGGGCAGCAT GATGGTGCTG AATCAGTTGA GCAACCTGGA GACCACAGTG 
GGCAGGTTCT ACACAAACCT TCCCAACCGG ATGATTGATG AAGCCGTCTT CAGCCTGCCC 
TTCTCTGATG AGATGGGAGA TGGTTTGATA ATGACTGTGA GTAAACCCTG TTATTTTGGA 
AACCTACTTC TGGGAATTGT AGGTGTGGAC GTGAATCTGG CTTACATTCT TGAAGACGTG 
ACGTATTACC AAGACTCTTT GGCTTCCTAT ACTTTTCTCA TAGACGACAA AGGATATACA 
CTTATGCACC CATCTCTTAC CAGGCCATAT TTATTGTCAG AGCCCCCACT TCATACTGAC 
ATCATACATT ATGAAAATAT TCCAAAATTT GAATTAGTTC GGCAAAATAT CCTAAGCCTC 
CCTCTGGGCA GCCAGATTAT CGCAGTCCCT GTGAACTCAT CCCTGTCTTG GCACATAAAC 
AAGCTGAGAG AAACTGGAAA GGAAGCCTAC AATGTTAGCT ATGCCTGGAA GATGGTACAA 
GACACTTCCT TTATTCTGTG TATTGTGGTG ATACAACCAG AAATACCTGT GAAACAACTG 
AAGAACCTCA ACACTGTTCC CAGCAGCAAG CTGCTGTACC ACCGGCTGGA TCTCCTTGGC 
CAGCCCAGTG CTTGCCTCCA CTTCAAACAG CTGGCAACCC TAGAAAGTCC CACCATCATG 
CTGTCTGCTG GCAGCTTTTC CTCCCCCTAT GAGCACCTCA GCCAGCCAGA GACAAAGCGC 
ATGGTAGAGC ACTACACCGC CTATCTCAGC GACAACACCC GCCTCATTGC TAACCCGGGC 
CTCAAATTCT CTGTCAGAAA TGAAGTAATG GCTACCAGCC ACGTCACAGA TGAATGGATG 
ACACAAATGG AAATGAGTAG CCTGAACACT TACATTGTCC GCCGTTACAT AGCAACACCC 
AATGGCGTCC TCAGAATTTA TCCTGGTTCC CTCATGGACA AAGCATTTGA TCCCACTAGG 
AGACAATGGT ATCTCCATGC AGTAGCTAAT CCAGGGTTGA TTTCTTTGAC TGGTCCTTAC 
TTAGATGTTG GAGGAGCTGG TTATGTTGTG ACAATCAGTC ACACAATTCA TTCATCCAGT 
ACACAGCTGT CTTCTGGGCA CACTGTGGCT GTGATGGGCA TTGACTTCAC ACTCAGATAC 
TTCTACAAAG TTCTGATGGA CCTATTACCT GTCTGTAACC AAGATGGTGG CAACAAAATA 
AGGTGCTTCA TAATGGAGGA CAGGGGTTAT CTGGTGGCGC ACCCGACTCT CATCGACCCC 
AAAGGACATG CACCTGTGGA GCAGCAGCAC ATCACCCACA AGGAGCCCCT GGTAGCAAAT 
GATATCCTCA ACCACCCCAA CTTTGTAAAG AAAAACCTGT GCAACAGCTT CAGTGACAGA 
ACGGTCCAGA GGTTTTATAA ATTCAACACC AGCCTTGCGG GGGATTTGAC GAACCTTGTG 
CATGGCAGCC ACTGTTCCAA ATACAGATTA GCAAGGATCC CAGGAACCAA CGCGTTTGTT 
GGCATTGTCA ACGAAACCTG CGACTCTCTT GCCTTCTGTG CCTGCAGCAT GGTGGACCGA 
CTCTGTCTCA ACTGTCACCG AATGGAACAA AATGAATGTG AATGTCCTTG TGAGTGCCCT 
CTAGAGGTCA ATGAGTGCAC TGGCAACCTC ACCAATGCAG AGAACCGAAA CCCCAGCTGC 
GAGGTCCACC AGGAGCCGGT GACATACACA GCTATTGACC CTGGCCTGCA AGATGCTCTT 
CACCAGTGTG TCAACAGCAG GTGCAGTCAG AGGCTGGAAA GTGGGGACTG TTTTGGGGTG 
CTGGATTGTG AATGGTGCAT GGTGGACAGT GATGGAAAGA CTCACCTGGA CAAACCCTAC 
TGTGCCCCCC AGAAAGAATG CTTCGGGGGG ATTGTGGGAG CCAAAAGTCC CTACGTTGAT 
GACATGGGAG CAATAGGTGA TGAGGTGATC ACATTAAACA TGATTAAAAG CGCCCCTGTG 
GGTCCTGTGG CTGGAGGGAT CATGGGATGC ATCATGGTCT TGGTCCTGGC GGTGTATGCC 
TACCGCCACC AGATTCATCG CCGGAGCCAT CAGCATATGT CTCCTCTTGC TGCCCAAGAA 
ATGTCAGTGC GTATGTCCAA CCTGGAGAAT GACAGAGATG AAAGGGACGA CGACAGCCAC 
GAAGACAGAG GCATCATCAG CAACACTCGG TTTATAGCTG CGGTCATCGA ACGACATGCA 
CACAGTCCAG AAAGAAGGCG CCGCTACTGG GGTCGATCAG GAACAGAAAG TGATCATGGT 
TACAGCACCA TGAGCCCACA GGAGGACAGT GAAAATCCTC CATGCAACAA TGACCCCTTG 
TCAGCCGGGG TCGATGTGGG AAACCATGAT GAGGACTTAG ACCTGGATAC CCCCCCTCAG 
ACTGCTGCCC TACTAAGTCA CAAGTTCCAC CACTACCGGT CACACCACCC TACACTTCAT 
CATAGCCACC ACTTACAGGC GGCCGTCACG GTACACACTG TCGATGCAGA ATGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001280203.1
CDS1120..4056
Translation

Target ORF information:

RefSeq Version NM_001293274.1
Organism Homo sapiens(human)
Definition Homo sapiens cache domain containing 1 (CACHD1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001293274.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
ATGTCCACCT TTGTTAGCAG CGTGAAGTCT TCAGACAGTC CTACCCAGCA CGCAGTGGGA 
TTCCAAAAGG CATTTCAGCT GATTCGAAGT ACAAACAATA ACACAAAGTT CCAAGCAAAT 
ACAGACATGG TCATCATTTA CCTGTCAGCT GGCATTACAT CAAAGGACTC TTCGGAAGAA 
GATAAAAAAG CGACTCTCCA AGTCATCAAT GAAGAAAATA GCTTTCTAAA CAACTCTGTA 
ATGATTCTCA CCTATGCCCT CATGAACGAT GGGGTGACTG GTTTGAAAGA GCTGGCTTTT 
CTGAGGGATC TAGCTGAACA GAATTCAGGG AAGTACGGTG TGCCAGACCG GATGGCCTTG 
CCTGTGATTA AGGGCAGCAT GATGGTGCTG AATCAGTTGA GCAACCTGGA GACCACAGTG 
GGCAGGTTCT ACACAAACCT TCCCAACCGG ATGATTGATG AAGCCGTCTT CAGCCTGCCC 
TTCTCTGATG AGATGGGAGA TGGTTTGATA ATGACTGTGA GTAAACCCTG TTATTTTGGA 
AACCTACTTC TGGGAATTGT AGGTGTGGAC GTGAATCTGG CTTACATTCT TGAAGACGTG 
ACGTATTACC AAGACTCTTT GGCTTCCTAT ACTTTTCTCA TAGACGACAA AGGATATACA 
CTTATGCACC CATCTCTTAC CAGGCCATAT TTATTGTCAG AGCCCCCACT TCATACTGAC 
ATCATACATT ATGAAAATAT TCCAAAATTT GAATTAGTTC GGCAAAATAT CCTAAGCCTC 
CCTCTGGGCA GCCAGATTAT CGCAGTCCCT GTGAACTCAT CCCTGTCTTG GCACATAAAC 
AAGCTGAGAG AAACTGGAAA GGAAGCCTAC AATGTTAGCT ATGCCTGGAA GATGGTACAA 
GACACTTCCT TTATTCTGTG TATTGTGGTG ATACAACCAG AAATACCTGT GAAACAACTG 
AAGAACCTCA ACACTGTTCC CAGCAGCAAG CTGCTGTACC ACCGGCTGGA TCTCCTTGGC 
CAGCCCAGTG CTTGCCTCCA CTTCAAACAG CTGGCAACCC TAGAAAGTCC CACCATCATG 
CTGTCTGCTG GCAGCTTTTC CTCCCCCTAT GAGCACCTCA GCCAGCCAGA GACAAAGCGC 
ATGGTAGAGC ACTACACCGC CTATCTCAGC GACAACACCC GCCTCATTGC TAACCCGGGC 
CTCAAATTCT CTGTCAGAAA TGAAGTAATG GCTACCAGCC ACGTCACAGA TGAATGGATG 
ACACAAATGG AAATGAGTAG CCTGAACACT TACATTGTCC GCCGTTACAT AGCAACACCC 
AATGGCGTCC TCAGAATTTA TCCTGGTTCC CTCATGGACA AAGCATTTGA TCCCACTAGG 
AGACAATGGT ATCTCCATGC AGTAGCTAAT CCAGGGTTGA TTTCTTTGAC TGGTCCTTAC 
TTAGATGTTG GAGGAGCTGG TTATGTTGTG ACAATCAGTC ACACAATTCA TTCATCCAGT 
ACACAGCTGT CTTCTGGGCA CACTGTGGCT GTGATGGGCA TTGACTTCAC ACTCAGATAC 
TTCTACAAAG TTCTGATGGA CCTATTACCT GTCTGTAACC AAGATGGTGG CAACAAAATA 
AGGTGCTTCA TAATGGAGGA CAGGGGTTAT CTGGTGGCGC ACCCGACTCT CATCGACCCC 
AAAGGACATG CACCTGTGGA GCAGCAGCAC ATCACCCACA AGGAGCCCCT GGTAGCAAAT 
GATATCCTCA ACCACCCCAA CTTTGTAAAG AAAAACCTGT GCAACAGCTT CAGTGACAGA 
ACGGTCCAGA GGTTTTATAA ATTCAACACC AGCCTTGCGG GGGATTTGAC GAACCTTGTG 
CATGGCAGCC ACTGTTCCAA ATACAGATTA GCAAGGATCC CAGGAACCAA CGCGTTTGTT 
GGCATTGTCA ACGAAACCTG CGACTCTCTT GCCTTCTGTG CCTGCAGCAT GGTGGACCGA 
CTCTGTCTCA ACTGTCACCG AATGGAACAA AATGAATGTG AATGTCCTTG TGAGTGCCCT 
CTAGAGGTCA ATGAGTGCAC TGGCAACCTC ACCAATGCAG AGAACCGAAA CCCCAGCTGC 
GAGGTCCACC AGGAGCCGGT GACATACACA GCTATTGACC CTGGCCTGCA AGATGCTCTT 
CACCAGTGTG TCAACAGCAG GTGCAGTCAG AGGCTGGAAA GTGGGGACTG TTTTGGGGTG 
CTGGATTGTG AATGGTGCAT GGTGGACAGT GATGGAAAGA CTCACCTGGA CAAACCCTAC 
TGTGCCCCCC AGAAAGAATG CTTCGGGGGG ATTGTGGGAG CCAAAAGTCC CTACGTTGAT 
GACATGGGAG CAATAGGTGA TGAGGTGATC ACATTAAACA TGATTAAAAG CGCCCCTGTG 
GGTCCTGTGG CTGGAGGGAT CATGGGATGC ATCATGGTCT TGGTCCTGGC GGTGTATGCC 
TACCGCCACC AGATTCATCG CCGGAGCCAT CAGCATATGT CTCCTCTTGC TGCCCAAGAA 
ATGTCAGTGC GTATGTCCAA CCTGGAGAAT GACAGAGATG AAAGGGACGA CGACAGCCAC 
GAAGACAGAG GCATCATCAG CAACACTCGG TTTATAGCTG CGGTCATCGA ACGACATGCA 
CACAGTCCAG AAAGAAGGCG CCGCTACTGG GGTCGATCAG GAACAGAAAG TGATCATGGT 
TACAGCACCA TGAGCCCACA GGAGGACAGT GAAAATCCTC CATGCAACAA TGACCCCTTG 
TCAGCCGGGG TCGATGTGGG AAACCATGAT GAGGACTTAG ACCTGGATAC CCCCCCTCAG 
ACTGCTGCCC TACTAAGTCA CAAGTTCCAC CACTACCGGT CACACCACCC TACACTTCAT 
CATAGCCACC ACTTACAGGC GGCCGTCACG GTACACACTG TCGATGCAGA ATGCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu106935
Clone ID Related Accession (Same CDS sequence) NM_020925.3 , NM_020925.4
Accession Version NM_020925.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-02-27
Organism Homo sapiens(human)
Product VWFA and cache domain-containing protein 1 isoform 1 precursor
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355515.12, AL590405.19 and AC099678.2. On Jul 3, 2018 this sequence version replaced NM_020925.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: This CCDS was updated to extend the coding region at the 5' end, which extends the N-terminus of the protein by 51aa. The update is supported by cross-species conservation data. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.156888.1, SRR1660803.223274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCCCGCC AGCCGGAGGA AGAGGAGACG GCCGTGGCCC GGGCGCGGCG GCCGCCCCTC 
TGGCTGCTCT GCCTGGTCGC GTGCTGGCTC CTGGGCGCCG GGGCCGAAGC CGACTTCTCC 
ATCCTGGACG AGGCGCAAGT GCTGGCGAGC CAGATGCGGA GGCTGGCGGC CGAGGAGCTG 
GGGGTCGTCA CCATGCAGCG GATATTCAAC TCCTTTGTTT ACACTGAGAA AATCTCAAAT 
GGAGAAAGTG AAGTACAGCA GCTAGCCAAA AAAATCCGAG AGAAGTTCAA CCGTTACTTG 
GATGTGGTCA ATCGGAACAA GCAAGTTGTA GAAGCATCCT ATACGGCTCA CCTAACCTCT 
CCCCTAACTG CAATTCAAGA CTGCTGTACT ATCCCACCTT CCATGATGGA ATTCGATGGG 
AACTTTAATA CCAATGTGTC TAGAACAATT AGTTGTGATC GACTTTCTAC TACTGTTAAT 
AGCCGGGCCT TCAATCCAGG ACGAGACTTA AATTCAGTTC TTGCAGACAA CCTGAAATCC 
AACCCTGGAA TTAAGTGGCA ATATTTCAGT TCAGAAGAAG GAATTTTCAC TGTTTTCCCA 
GCACACAAGT TCCGGTGTAA GGGCAGCTAC GAACACCGCA GTAGACCCAT CTACGTCTCT 
ACAGTCCGGC CGCAGTCAAA GCACATAGTA GTGATTCTGG ACCACGGGGC TTCAGTCACA 
GACACTCAGC TTCAGATTGC CAAGGACGCT GCTCAGGTCA TCCTCAGCGC CATCGATGAA 
CATGACAAGA TTTCTGTGTT AACTGTGGCA GATACCGTCC GGACTTGCTC ACTAGACCAG 
TGCTATAAGA CCTTCTTGTC TCCAGCCACC AGTGAGACAA AAAGGAAAAT GTCCACCTTT 
GTTAGCAGCG TGAAGTCTTC AGACAGTCCT ACCCAGCACG CAGTGGGATT CCAAAAGGCA 
TTTCAGCTGA TTCGAAGTAC AAACAATAAC ACAAAGTTCC AAGCAAATAC AGACATGGTC 
ATCATTTACC TGTCAGCTGG CATTACATCA AAGGACTCTT CGGAAGAAGA TAAAAAAGCG 
ACTCTCCAAG TCATCAATGA AGAAAATAGC TTTCTAAACA ACTCTGTAAT GATTCTCACC 
TATGCCCTCA TGAACGATGG GGTGACTGGT TTGAAAGAGC TGGCTTTTCT GAGGGATCTA 
GCTGAACAGA ATTCAGGGAA GTACGGTGTG CCAGACCGGA TGGCCTTGCC TGTGATTAAG 
GGCAGCATGA TGGTGCTGAA TCAGTTGAGC AACCTGGAGA CCACAGTGGG CAGGTTCTAC 
ACAAACCTTC CCAACCGGAT GATTGATGAA GCCGTCTTCA GCCTGCCCTT CTCTGATGAG 
ATGGGAGATG GTTTGATAAT GACTGTGAGT AAACCCTGTT ATTTTGGAAA CCTACTTCTG 
GGAATTGTAG GTGTGGACGT GAATCTGGCT TACATTCTTG AAGACGTGAC GTATTACCAA 
GACTCTTTGG CTTCCTATAC TTTTCTCATA GACGACAAAG GATATACACT TATGCACCCA 
TCTCTTACCA GGCCATATTT ATTGTCAGAG CCCCCACTTC ATACTGACAT CATACATTAT 
GAAAATATTC CAAAATTTGA ATTAGTTCGG CAAAATATCC TAAGCCTCCC TCTGGGCAGC 
CAGATTATCG CAGTCCCTGT GAACTCATCC CTGTCTTGGC ACATAAACAA GCTGAGAGAA 
ACTGGAAAGG AAGCCTACAA TGTTAGCTAT GCCTGGAAGA TGGTACAAGA CACTTCCTTT 
ATTCTGTGTA TTGTGGTGAT ACAACCAGAA ATACCTGTGA AACAACTGAA GAACCTCAAC 
ACTGTTCCCA GCAGCAAGCT GCTGTACCAC CGGCTGGATC TCCTTGGCCA GCCCAGTGCT 
TGCCTCCACT TCAAACAGCT GGCAACCCTA GAAAGTCCCA CCATCATGCT GTCTGCTGGC 
AGCTTTTCCT CCCCCTATGA GCACCTCAGC CAGCCAGAGA CAAAGCGCAT GGTAGAGCAC 
TACACCGCCT ATCTCAGCGA CAACACCCGC CTCATTGCTA ACCCGGGCCT CAAATTCTCT 
GTCAGAAATG AAGTAATGGC TACCAGCCAC GTCACAGATG AATGGATGAC ACAAATGGAA 
ATGAGTAGCC TGAACACTTA CATTGTCCGC CGTTACATAG CAACACCCAA TGGCGTCCTC 
AGAATTTATC CTGGTTCCCT CATGGACAAA GCATTTGATC CCACTAGGAG ACAATGGTAT 
CTCCATGCAG TAGCTAATCC AGGGTTGATT TCTTTGACTG GTCCTTACTT AGATGTTGGA 
GGAGCTGGTT ATGTTGTGAC AATCAGTCAC ACAATTCATT CATCCAGTAC ACAGCTGTCT 
TCTGGGCACA CTGTGGCTGT GATGGGCATT GACTTCACAC TCAGATACTT CTACAAAGTT 
CTGATGGACC TATTACCTGT CTGTAACCAA GATGGTGGCA ACAAAATAAG GTGCTTCATA 
ATGGAGGACA GGGGTTATCT GGTGGCGCAC CCGACTCTCA TCGACCCCAA AGGACATGCA 
CCTGTGGAGC AGCAGCACAT CACCCACAAG GAGCCCCTGG TAGCAAATGA TATCCTCAAC 
CACCCCAACT TTGTAAAGAA AAACCTGTGC AACAGCTTCA GTGACAGAAC GGTCCAGAGG 
TTTTATAAAT TCAACACCAG CCTTGCGGGG GATTTGACGA ACCTTGTGCA TGGCAGCCAC 
TGTTCCAAAT ACAGATTAGC AAGGATCCCA GGAACCAACG CGTTTGTTGG CATTGTCAAC 
GAAACCTGCG ACTCTCTTGC CTTCTGTGCC TGCAGCATGG TGGACCGACT CTGTCTCAAC 
TGTCACCGAA TGGAACAAAA TGAATGTGAA TGTCCTTGTG AGTGCCCTCT AGAGGTCAAT 
GAGTGCACTG GCAACCTCAC CAATGCAGAG AACCGAAACC CCAGCTGCGA GGTCCACCAG 
GAGCCGGTGA CATACACAGC TATTGACCCT GGCCTGCAAG ATGCTCTTCA CCAGTGTGTC 
AACAGCAGGT GCAGTCAGAG GCTGGAAAGT GGGGACTGTT TTGGGGTGCT GGATTGTGAA 
TGGTGCATGG TGGACAGTGA TGGAAAGACT CACCTGGACA AACCCTACTG TGCCCCCCAG 
AAAGAATGCT TCGGGGGGAT TGTGGGAGCC AAAAGTCCCT ACGTTGATGA CATGGGAGCA 
ATAGGTGATG AGGTGATCAC ATTAAACATG ATTAAAAGCG CCCCTGTGGG TCCTGTGGCT 
GGAGGGATCA TGGGATGCAT CATGGTCTTG GTCCTGGCGG TGTATGCCTA CCGCCACCAG 
ATTCATCGCC GGAGCCATCA GCATATGTCT CCTCTTGCTG CCCAAGAAAT GTCAGTGCGT 
ATGTCCAACC TGGAGAATGA CAGAGATGAA AGGGACGACG ACAGCCACGA AGACAGAGGC 
ATCATCAGCA ACACTCGGTT TATAGCTGCG GTCATCGAAC GACATGCACA CAGTCCAGAA 
AGAAGGCGCC GCTACTGGGG TCGATCAGGA ACAGAAAGTG ATCATGGTTA CAGCACCATG 
AGCCCACAGG AGGACAGTGA AAATCCTCCA TGCAACAATG ACCCCTTGTC AGCCGGGGTC 
GATGTGGGAA ACCATGATGA GGACTTAGAC CTGGATACCC CCCCTCAGAC TGCTGCCCTA 
CTAAGTCACA AGTTCCACCA CTACCGGTCA CACCACCCTA CACTTCATCA TAGCCACCAC 
TTACAGGCGG CCGTCACGGT ACACACTGTC GATGCAGAAT GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_065976.3
CDS617..4441
Translation

Target ORF information:

RefSeq Version NM_020925.3
Organism Homo sapiens(human)
Definition Homo sapiens cache domain containing 1 (CACHD1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_020925.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCCCGCC AGCCGGAGGA AGAGGAGACG GCCGTGGCCC GGGCGCGGCG GCCGCCCCTC 
TGGCTGCTCT GCCTGGTCGC GTGCTGGCTC CTGGGCGCCG GGGCCGAAGC CGACTTCTCC 
ATCCTGGACG AGGCGCAAGT GCTGGCGAGC CAGATGCGGA GGCTGGCGGC CGAGGAGCTG 
GGGGTCGTCA CCATGCAGCG GATATTCAAC TCCTTTGTTT ACACTGAGAA AATCTCAAAT 
GGAGAAAGTG AAGTACAGCA GCTAGCCAAA AAAATCCGAG AGAAGTTCAA CCGTTACTTG 
GATGTGGTCA ATCGGAACAA GCAAGTTGTA GAAGCATCCT ATACGGCTCA CCTAACCTCT 
CCCCTAACTG CAATTCAAGA CTGCTGTACT ATCCCACCTT CCATGATGGA ATTCGATGGG 
AACTTTAATA CCAATGTGTC TAGAACAATT AGTTGTGATC GACTTTCTAC TACTGTTAAT 
AGCCGGGCCT TCAATCCAGG ACGAGACTTA AATTCAGTTC TTGCAGACAA CCTGAAATCC 
AACCCTGGAA TTAAGTGGCA ATATTTCAGT TCAGAAGAAG GAATTTTCAC TGTTTTCCCA 
GCACACAAGT TCCGGTGTAA GGGCAGCTAC GAACACCGCA GTAGACCCAT CTACGTCTCT 
ACAGTCCGGC CGCAGTCAAA GCACATAGTA GTGATTCTGG ACCACGGGGC TTCAGTCACA 
GACACTCAGC TTCAGATTGC CAAGGACGCT GCTCAGGTCA TCCTCAGCGC CATCGATGAA 
CATGACAAGA TTTCTGTGTT AACTGTGGCA GATACCGTCC GGACTTGCTC ACTAGACCAG 
TGCTATAAGA CCTTCTTGTC TCCAGCCACC AGTGAGACAA AAAGGAAAAT GTCCACCTTT 
GTTAGCAGCG TGAAGTCTTC AGACAGTCCT ACCCAGCACG CAGTGGGATT CCAAAAGGCA 
TTTCAGCTGA TTCGAAGTAC AAACAATAAC ACAAAGTTCC AAGCAAATAC AGACATGGTC 
ATCATTTACC TGTCAGCTGG CATTACATCA AAGGACTCTT CGGAAGAAGA TAAAAAAGCG 
ACTCTCCAAG TCATCAATGA AGAAAATAGC TTTCTAAACA ACTCTGTAAT GATTCTCACC 
TATGCCCTCA TGAACGATGG GGTGACTGGT TTGAAAGAGC TGGCTTTTCT GAGGGATCTA 
GCTGAACAGA ATTCAGGGAA GTACGGTGTG CCAGACCGGA TGGCCTTGCC TGTGATTAAG 
GGCAGCATGA TGGTGCTGAA TCAGTTGAGC AACCTGGAGA CCACAGTGGG CAGGTTCTAC 
ACAAACCTTC CCAACCGGAT GATTGATGAA GCCGTCTTCA GCCTGCCCTT CTCTGATGAG 
ATGGGAGATG GTTTGATAAT GACTGTGAGT AAACCCTGTT ATTTTGGAAA CCTACTTCTG 
GGAATTGTAG GTGTGGACGT GAATCTGGCT TACATTCTTG AAGACGTGAC GTATTACCAA 
GACTCTTTGG CTTCCTATAC TTTTCTCATA GACGACAAAG GATATACACT TATGCACCCA 
TCTCTTACCA GGCCATATTT ATTGTCAGAG CCCCCACTTC ATACTGACAT CATACATTAT 
GAAAATATTC CAAAATTTGA ATTAGTTCGG CAAAATATCC TAAGCCTCCC TCTGGGCAGC 
CAGATTATCG CAGTCCCTGT GAACTCATCC CTGTCTTGGC ACATAAACAA GCTGAGAGAA 
ACTGGAAAGG AAGCCTACAA TGTTAGCTAT GCCTGGAAGA TGGTACAAGA CACTTCCTTT 
ATTCTGTGTA TTGTGGTGAT ACAACCAGAA ATACCTGTGA AACAACTGAA GAACCTCAAC 
ACTGTTCCCA GCAGCAAGCT GCTGTACCAC CGGCTGGATC TCCTTGGCCA GCCCAGTGCT 
TGCCTCCACT TCAAACAGCT GGCAACCCTA GAAAGTCCCA CCATCATGCT GTCTGCTGGC 
AGCTTTTCCT CCCCCTATGA GCACCTCAGC CAGCCAGAGA CAAAGCGCAT GGTAGAGCAC 
TACACCGCCT ATCTCAGCGA CAACACCCGC CTCATTGCTA ACCCGGGCCT CAAATTCTCT 
GTCAGAAATG AAGTAATGGC TACCAGCCAC GTCACAGATG AATGGATGAC ACAAATGGAA 
ATGAGTAGCC TGAACACTTA CATTGTCCGC CGTTACATAG CAACACCCAA TGGCGTCCTC 
AGAATTTATC CTGGTTCCCT CATGGACAAA GCATTTGATC CCACTAGGAG ACAATGGTAT 
CTCCATGCAG TAGCTAATCC AGGGTTGATT TCTTTGACTG GTCCTTACTT AGATGTTGGA 
GGAGCTGGTT ATGTTGTGAC AATCAGTCAC ACAATTCATT CATCCAGTAC ACAGCTGTCT 
TCTGGGCACA CTGTGGCTGT GATGGGCATT GACTTCACAC TCAGATACTT CTACAAAGTT 
CTGATGGACC TATTACCTGT CTGTAACCAA GATGGTGGCA ACAAAATAAG GTGCTTCATA 
ATGGAGGACA GGGGTTATCT GGTGGCGCAC CCGACTCTCA TCGACCCCAA AGGACATGCA 
CCTGTGGAGC AGCAGCACAT CACCCACAAG GAGCCCCTGG TAGCAAATGA TATCCTCAAC 
CACCCCAACT TTGTAAAGAA AAACCTGTGC AACAGCTTCA GTGACAGAAC GGTCCAGAGG 
TTTTATAAAT TCAACACCAG CCTTGCGGGG GATTTGACGA ACCTTGTGCA TGGCAGCCAC 
TGTTCCAAAT ACAGATTAGC AAGGATCCCA GGAACCAACG CGTTTGTTGG CATTGTCAAC 
GAAACCTGCG ACTCTCTTGC CTTCTGTGCC TGCAGCATGG TGGACCGACT CTGTCTCAAC 
TGTCACCGAA TGGAACAAAA TGAATGTGAA TGTCCTTGTG AGTGCCCTCT AGAGGTCAAT 
GAGTGCACTG GCAACCTCAC CAATGCAGAG AACCGAAACC CCAGCTGCGA GGTCCACCAG 
GAGCCGGTGA CATACACAGC TATTGACCCT GGCCTGCAAG ATGCTCTTCA CCAGTGTGTC 
AACAGCAGGT GCAGTCAGAG GCTGGAAAGT GGGGACTGTT TTGGGGTGCT GGATTGTGAA 
TGGTGCATGG TGGACAGTGA TGGAAAGACT CACCTGGACA AACCCTACTG TGCCCCCCAG 
AAAGAATGCT TCGGGGGGAT TGTGGGAGCC AAAAGTCCCT ACGTTGATGA CATGGGAGCA 
ATAGGTGATG AGGTGATCAC ATTAAACATG ATTAAAAGCG CCCCTGTGGG TCCTGTGGCT 
GGAGGGATCA TGGGATGCAT CATGGTCTTG GTCCTGGCGG TGTATGCCTA CCGCCACCAG 
ATTCATCGCC GGAGCCATCA GCATATGTCT CCTCTTGCTG CCCAAGAAAT GTCAGTGCGT 
ATGTCCAACC TGGAGAATGA CAGAGATGAA AGGGACGACG ACAGCCACGA AGACAGAGGC 
ATCATCAGCA ACACTCGGTT TATAGCTGCG GTCATCGAAC GACATGCACA CAGTCCAGAA 
AGAAGGCGCC GCTACTGGGG TCGATCAGGA ACAGAAAGTG ATCATGGTTA CAGCACCATG 
AGCCCACAGG AGGACAGTGA AAATCCTCCA TGCAACAATG ACCCCTTGTC AGCCGGGGTC 
GATGTGGGAA ACCATGATGA GGACTTAGAC CTGGATACCC CCCCTCAGAC TGCTGCCCTA 
CTAAGTCACA AGTTCCACCA CTACCGGTCA CACCACCCTA CACTTCATCA TAGCCACCAC 
TTACAGGCGG CCGTCACGGT ACACACTGTC GATGCAGAAT GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu106935
Clone ID Related Accession (Same CDS sequence) NM_020925.3 , NM_020925.4
Accession Version NM_020925.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-13
Organism Homo sapiens(human)
Product VWFA and cache domain-containing protein 1 isoform 1 precursor
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355515.12, AL590405.19 and AC099678.2. On May 17, 2019 this sequence version replaced NM_020925.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: This CCDS was updated to extend the coding region at the 5' end, which extends the N-terminus of the protein by 51aa. The update is supported by cross-species conservation data. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.156888.1, SRR1660803.223274.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000651257.2/ ENSP00000498498.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCCCGCC AGCCGGAGGA AGAGGAGACG GCCGTGGCCC GGGCGCGGCG GCCGCCCCTC 
TGGCTGCTCT GCCTGGTCGC GTGCTGGCTC CTGGGCGCCG GGGCCGAAGC CGACTTCTCC 
ATCCTGGACG AGGCGCAAGT GCTGGCGAGC CAGATGCGGA GGCTGGCGGC CGAGGAGCTG 
GGGGTCGTCA CCATGCAGCG GATATTCAAC TCCTTTGTTT ACACTGAGAA AATCTCAAAT 
GGAGAAAGTG AAGTACAGCA GCTAGCCAAA AAAATCCGAG AGAAGTTCAA CCGTTACTTG 
GATGTGGTCA ATCGGAACAA GCAAGTTGTA GAAGCATCCT ATACGGCTCA CCTAACCTCT 
CCCCTAACTG CAATTCAAGA CTGCTGTACT ATCCCACCTT CCATGATGGA ATTCGATGGG 
AACTTTAATA CCAATGTGTC TAGAACAATT AGTTGTGATC GACTTTCTAC TACTGTTAAT 
AGCCGGGCCT TCAATCCAGG ACGAGACTTA AATTCAGTTC TTGCAGACAA CCTGAAATCC 
AACCCTGGAA TTAAGTGGCA ATATTTCAGT TCAGAAGAAG GAATTTTCAC TGTTTTCCCA 
GCACACAAGT TCCGGTGTAA GGGCAGCTAC GAACACCGCA GTAGACCCAT CTACGTCTCT 
ACAGTCCGGC CGCAGTCAAA GCACATAGTA GTGATTCTGG ACCACGGGGC TTCAGTCACA 
GACACTCAGC TTCAGATTGC CAAGGACGCT GCTCAGGTCA TCCTCAGCGC CATCGATGAA 
CATGACAAGA TTTCTGTGTT AACTGTGGCA GATACCGTCC GGACTTGCTC ACTAGACCAG 
TGCTATAAGA CCTTCTTGTC TCCAGCCACC AGTGAGACAA AAAGGAAAAT GTCCACCTTT 
GTTAGCAGCG TGAAGTCTTC AGACAGTCCT ACCCAGCACG CAGTGGGATT CCAAAAGGCA 
TTTCAGCTGA TTCGAAGTAC AAACAATAAC ACAAAGTTCC AAGCAAATAC AGACATGGTC 
ATCATTTACC TGTCAGCTGG CATTACATCA AAGGACTCTT CGGAAGAAGA TAAAAAAGCG 
ACTCTCCAAG TCATCAATGA AGAAAATAGC TTTCTAAACA ACTCTGTAAT GATTCTCACC 
TATGCCCTCA TGAACGATGG GGTGACTGGT TTGAAAGAGC TGGCTTTTCT GAGGGATCTA 
GCTGAACAGA ATTCAGGGAA GTACGGTGTG CCAGACCGGA TGGCCTTGCC TGTGATTAAG 
GGCAGCATGA TGGTGCTGAA TCAGTTGAGC AACCTGGAGA CCACAGTGGG CAGGTTCTAC 
ACAAACCTTC CCAACCGGAT GATTGATGAA GCCGTCTTCA GCCTGCCCTT CTCTGATGAG 
ATGGGAGATG GTTTGATAAT GACTGTGAGT AAACCCTGTT ATTTTGGAAA CCTACTTCTG 
GGAATTGTAG GTGTGGACGT GAATCTGGCT TACATTCTTG AAGACGTGAC GTATTACCAA 
GACTCTTTGG CTTCCTATAC TTTTCTCATA GACGACAAAG GATATACACT TATGCACCCA 
TCTCTTACCA GGCCATATTT ATTGTCAGAG CCCCCACTTC ATACTGACAT CATACATTAT 
GAAAATATTC CAAAATTTGA ATTAGTTCGG CAAAATATCC TAAGCCTCCC TCTGGGCAGC 
CAGATTATCG CAGTCCCTGT GAACTCATCC CTGTCTTGGC ACATAAACAA GCTGAGAGAA 
ACTGGAAAGG AAGCCTACAA TGTTAGCTAT GCCTGGAAGA TGGTACAAGA CACTTCCTTT 
ATTCTGTGTA TTGTGGTGAT ACAACCAGAA ATACCTGTGA AACAACTGAA GAACCTCAAC 
ACTGTTCCCA GCAGCAAGCT GCTGTACCAC CGGCTGGATC TCCTTGGCCA GCCCAGTGCT 
TGCCTCCACT TCAAACAGCT GGCAACCCTA GAAAGTCCCA CCATCATGCT GTCTGCTGGC 
AGCTTTTCCT CCCCCTATGA GCACCTCAGC CAGCCAGAGA CAAAGCGCAT GGTAGAGCAC 
TACACCGCCT ATCTCAGCGA CAACACCCGC CTCATTGCTA ACCCGGGCCT CAAATTCTCT 
GTCAGAAATG AAGTAATGGC TACCAGCCAC GTCACAGATG AATGGATGAC ACAAATGGAA 
ATGAGTAGCC TGAACACTTA CATTGTCCGC CGTTACATAG CAACACCCAA TGGCGTCCTC 
AGAATTTATC CTGGTTCCCT CATGGACAAA GCATTTGATC CCACTAGGAG ACAATGGTAT 
CTCCATGCAG TAGCTAATCC AGGGTTGATT TCTTTGACTG GTCCTTACTT AGATGTTGGA 
GGAGCTGGTT ATGTTGTGAC AATCAGTCAC ACAATTCATT CATCCAGTAC ACAGCTGTCT 
TCTGGGCACA CTGTGGCTGT GATGGGCATT GACTTCACAC TCAGATACTT CTACAAAGTT 
CTGATGGACC TATTACCTGT CTGTAACCAA GATGGTGGCA ACAAAATAAG GTGCTTCATA 
ATGGAGGACA GGGGTTATCT GGTGGCGCAC CCGACTCTCA TCGACCCCAA AGGACATGCA 
CCTGTGGAGC AGCAGCACAT CACCCACAAG GAGCCCCTGG TAGCAAATGA TATCCTCAAC 
CACCCCAACT TTGTAAAGAA AAACCTGTGC AACAGCTTCA GTGACAGAAC GGTCCAGAGG 
TTTTATAAAT TCAACACCAG CCTTGCGGGG GATTTGACGA ACCTTGTGCA TGGCAGCCAC 
TGTTCCAAAT ACAGATTAGC AAGGATCCCA GGAACCAACG CGTTTGTTGG CATTGTCAAC 
GAAACCTGCG ACTCTCTTGC CTTCTGTGCC TGCAGCATGG TGGACCGACT CTGTCTCAAC 
TGTCACCGAA TGGAACAAAA TGAATGTGAA TGTCCTTGTG AGTGCCCTCT AGAGGTCAAT 
GAGTGCACTG GCAACCTCAC CAATGCAGAG AACCGAAACC CCAGCTGCGA GGTCCACCAG 
GAGCCGGTGA CATACACAGC TATTGACCCT GGCCTGCAAG ATGCTCTTCA CCAGTGTGTC 
AACAGCAGGT GCAGTCAGAG GCTGGAAAGT GGGGACTGTT TTGGGGTGCT GGATTGTGAA 
TGGTGCATGG TGGACAGTGA TGGAAAGACT CACCTGGACA AACCCTACTG TGCCCCCCAG 
AAAGAATGCT TCGGGGGGAT TGTGGGAGCC AAAAGTCCCT ACGTTGATGA CATGGGAGCA 
ATAGGTGATG AGGTGATCAC ATTAAACATG ATTAAAAGCG CCCCTGTGGG TCCTGTGGCT 
GGAGGGATCA TGGGATGCAT CATGGTCTTG GTCCTGGCGG TGTATGCCTA CCGCCACCAG 
ATTCATCGCC GGAGCCATCA GCATATGTCT CCTCTTGCTG CCCAAGAAAT GTCAGTGCGT 
ATGTCCAACC TGGAGAATGA CAGAGATGAA AGGGACGACG ACAGCCACGA AGACAGAGGC 
ATCATCAGCA ACACTCGGTT TATAGCTGCG GTCATCGAAC GACATGCACA CAGTCCAGAA 
AGAAGGCGCC GCTACTGGGG TCGATCAGGA ACAGAAAGTG ATCATGGTTA CAGCACCATG 
AGCCCACAGG AGGACAGTGA AAATCCTCCA TGCAACAATG ACCCCTTGTC AGCCGGGGTC 
GATGTGGGAA ACCATGATGA GGACTTAGAC CTGGATACCC CCCCTCAGAC TGCTGCCCTA 
CTAAGTCACA AGTTCCACCA CTACCGGTCA CACCACCCTA CACTTCATCA TAGCCACCAC 
TTACAGGCGG CCGTCACGGT ACACACTGTC GATGCAGAAT GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_065976.3
CDS617..4441
Translation

Target ORF information:

RefSeq Version NM_020925.4
Organism Homo sapiens(human)
Definition Homo sapiens cache domain containing 1 (CACHD1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_020925.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGCCCGCC AGCCGGAGGA AGAGGAGACG GCCGTGGCCC GGGCGCGGCG GCCGCCCCTC 
TGGCTGCTCT GCCTGGTCGC GTGCTGGCTC CTGGGCGCCG GGGCCGAAGC CGACTTCTCC 
ATCCTGGACG AGGCGCAAGT GCTGGCGAGC CAGATGCGGA GGCTGGCGGC CGAGGAGCTG 
GGGGTCGTCA CCATGCAGCG GATATTCAAC TCCTTTGTTT ACACTGAGAA AATCTCAAAT 
GGAGAAAGTG AAGTACAGCA GCTAGCCAAA AAAATCCGAG AGAAGTTCAA CCGTTACTTG 
GATGTGGTCA ATCGGAACAA GCAAGTTGTA GAAGCATCCT ATACGGCTCA CCTAACCTCT 
CCCCTAACTG CAATTCAAGA CTGCTGTACT ATCCCACCTT CCATGATGGA ATTCGATGGG 
AACTTTAATA CCAATGTGTC TAGAACAATT AGTTGTGATC GACTTTCTAC TACTGTTAAT 
AGCCGGGCCT TCAATCCAGG ACGAGACTTA AATTCAGTTC TTGCAGACAA CCTGAAATCC 
AACCCTGGAA TTAAGTGGCA ATATTTCAGT TCAGAAGAAG GAATTTTCAC TGTTTTCCCA 
GCACACAAGT TCCGGTGTAA GGGCAGCTAC GAACACCGCA GTAGACCCAT CTACGTCTCT 
ACAGTCCGGC CGCAGTCAAA GCACATAGTA GTGATTCTGG ACCACGGGGC TTCAGTCACA 
GACACTCAGC TTCAGATTGC CAAGGACGCT GCTCAGGTCA TCCTCAGCGC CATCGATGAA 
CATGACAAGA TTTCTGTGTT AACTGTGGCA GATACCGTCC GGACTTGCTC ACTAGACCAG 
TGCTATAAGA CCTTCTTGTC TCCAGCCACC AGTGAGACAA AAAGGAAAAT GTCCACCTTT 
GTTAGCAGCG TGAAGTCTTC AGACAGTCCT ACCCAGCACG CAGTGGGATT CCAAAAGGCA 
TTTCAGCTGA TTCGAAGTAC AAACAATAAC ACAAAGTTCC AAGCAAATAC AGACATGGTC 
ATCATTTACC TGTCAGCTGG CATTACATCA AAGGACTCTT CGGAAGAAGA TAAAAAAGCG 
ACTCTCCAAG TCATCAATGA AGAAAATAGC TTTCTAAACA ACTCTGTAAT GATTCTCACC 
TATGCCCTCA TGAACGATGG GGTGACTGGT TTGAAAGAGC TGGCTTTTCT GAGGGATCTA 
GCTGAACAGA ATTCAGGGAA GTACGGTGTG CCAGACCGGA TGGCCTTGCC TGTGATTAAG 
GGCAGCATGA TGGTGCTGAA TCAGTTGAGC AACCTGGAGA CCACAGTGGG CAGGTTCTAC 
ACAAACCTTC CCAACCGGAT GATTGATGAA GCCGTCTTCA GCCTGCCCTT CTCTGATGAG 
ATGGGAGATG GTTTGATAAT GACTGTGAGT AAACCCTGTT ATTTTGGAAA CCTACTTCTG 
GGAATTGTAG GTGTGGACGT GAATCTGGCT TACATTCTTG AAGACGTGAC GTATTACCAA 
GACTCTTTGG CTTCCTATAC TTTTCTCATA GACGACAAAG GATATACACT TATGCACCCA 
TCTCTTACCA GGCCATATTT ATTGTCAGAG CCCCCACTTC ATACTGACAT CATACATTAT 
GAAAATATTC CAAAATTTGA ATTAGTTCGG CAAAATATCC TAAGCCTCCC TCTGGGCAGC 
CAGATTATCG CAGTCCCTGT GAACTCATCC CTGTCTTGGC ACATAAACAA GCTGAGAGAA 
ACTGGAAAGG AAGCCTACAA TGTTAGCTAT GCCTGGAAGA TGGTACAAGA CACTTCCTTT 
ATTCTGTGTA TTGTGGTGAT ACAACCAGAA ATACCTGTGA AACAACTGAA GAACCTCAAC 
ACTGTTCCCA GCAGCAAGCT GCTGTACCAC CGGCTGGATC TCCTTGGCCA GCCCAGTGCT 
TGCCTCCACT TCAAACAGCT GGCAACCCTA GAAAGTCCCA CCATCATGCT GTCTGCTGGC 
AGCTTTTCCT CCCCCTATGA GCACCTCAGC CAGCCAGAGA CAAAGCGCAT GGTAGAGCAC 
TACACCGCCT ATCTCAGCGA CAACACCCGC CTCATTGCTA ACCCGGGCCT CAAATTCTCT 
GTCAGAAATG AAGTAATGGC TACCAGCCAC GTCACAGATG AATGGATGAC ACAAATGGAA 
ATGAGTAGCC TGAACACTTA CATTGTCCGC CGTTACATAG CAACACCCAA TGGCGTCCTC 
AGAATTTATC CTGGTTCCCT CATGGACAAA GCATTTGATC CCACTAGGAG ACAATGGTAT 
CTCCATGCAG TAGCTAATCC AGGGTTGATT TCTTTGACTG GTCCTTACTT AGATGTTGGA 
GGAGCTGGTT ATGTTGTGAC AATCAGTCAC ACAATTCATT CATCCAGTAC ACAGCTGTCT 
TCTGGGCACA CTGTGGCTGT GATGGGCATT GACTTCACAC TCAGATACTT CTACAAAGTT 
CTGATGGACC TATTACCTGT CTGTAACCAA GATGGTGGCA ACAAAATAAG GTGCTTCATA 
ATGGAGGACA GGGGTTATCT GGTGGCGCAC CCGACTCTCA TCGACCCCAA AGGACATGCA 
CCTGTGGAGC AGCAGCACAT CACCCACAAG GAGCCCCTGG TAGCAAATGA TATCCTCAAC 
CACCCCAACT TTGTAAAGAA AAACCTGTGC AACAGCTTCA GTGACAGAAC GGTCCAGAGG 
TTTTATAAAT TCAACACCAG CCTTGCGGGG GATTTGACGA ACCTTGTGCA TGGCAGCCAC 
TGTTCCAAAT ACAGATTAGC AAGGATCCCA GGAACCAACG CGTTTGTTGG CATTGTCAAC 
GAAACCTGCG ACTCTCTTGC CTTCTGTGCC TGCAGCATGG TGGACCGACT CTGTCTCAAC 
TGTCACCGAA TGGAACAAAA TGAATGTGAA TGTCCTTGTG AGTGCCCTCT AGAGGTCAAT 
GAGTGCACTG GCAACCTCAC CAATGCAGAG AACCGAAACC CCAGCTGCGA GGTCCACCAG 
GAGCCGGTGA CATACACAGC TATTGACCCT GGCCTGCAAG ATGCTCTTCA CCAGTGTGTC 
AACAGCAGGT GCAGTCAGAG GCTGGAAAGT GGGGACTGTT TTGGGGTGCT GGATTGTGAA 
TGGTGCATGG TGGACAGTGA TGGAAAGACT CACCTGGACA AACCCTACTG TGCCCCCCAG 
AAAGAATGCT TCGGGGGGAT TGTGGGAGCC AAAAGTCCCT ACGTTGATGA CATGGGAGCA 
ATAGGTGATG AGGTGATCAC ATTAAACATG ATTAAAAGCG CCCCTGTGGG TCCTGTGGCT 
GGAGGGATCA TGGGATGCAT CATGGTCTTG GTCCTGGCGG TGTATGCCTA CCGCCACCAG 
ATTCATCGCC GGAGCCATCA GCATATGTCT CCTCTTGCTG CCCAAGAAAT GTCAGTGCGT 
ATGTCCAACC TGGAGAATGA CAGAGATGAA AGGGACGACG ACAGCCACGA AGACAGAGGC 
ATCATCAGCA ACACTCGGTT TATAGCTGCG GTCATCGAAC GACATGCACA CAGTCCAGAA 
AGAAGGCGCC GCTACTGGGG TCGATCAGGA ACAGAAAGTG ATCATGGTTA CAGCACCATG 
AGCCCACAGG AGGACAGTGA AAATCCTCCA TGCAACAATG ACCCCTTGTC AGCCGGGGTC 
GATGTGGGAA ACCATGATGA GGACTTAGAC CTGGATACCC CCCCTCAGAC TGCTGCCCTA 
CTAAGTCACA AGTTCCACCA CTACCGGTCA CACCACCCTA CACTTCATCA TAGCCACCAC 
TTACAGGCGG CCGTCACGGT ACACACTGTC GATGCAGAAT GCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27880
Clone ID Related Accession (Same CDS sequence) NM_020925.2
Accession Version NM_020925.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3672bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-10-29
Organism Homo sapiens(human)
Product VWFA and cache domain-containing protein 1 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL355515.12, CX163220.1, AB046793.1, AL590405.19 and AC099678.2. On Jul 20, 2006 this sequence version replaced NM_020925.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCGGAGGC TGGCGGCCGA GGAGCTGGGG GTCGTCACCA TGCAGCGGAT ATTCAACTCC 
TTTGTTTACA CTGAGAAAAT CTCAAATGGA GAAAGTGAAG TACAGCAGCT AGCCAAAAAA 
ATCCGAGAGA AGTTCAACCG TTACTTGGAT GTGGTCAATC GGAACAAGCA AGTTGTAGAA 
GCATCCTATA CGGCTCACCT AACCTCTCCC CTAACTGCAA TTCAAGACTG CTGTACTATC 
CCACCTTCCA TGATGGAATT CGATGGGAAC TTTAATACCA ATGTGTCTAG AACAATTAGT 
TGTGATCGAC TTTCTACTAC TGTTAATAGC CGGGCCTTCA ATCCAGGACG AGACTTAAAT 
TCAGTTCTTG CAGACAACCT GAAATCCAAC CCTGGAATTA AGTGGCAATA TTTCAGTTCA 
GAAGAAGGAA TTTTCACTGT TTTCCCAGCA CACAAGTTCC GGTGTAAGGG CAGCTACGAA 
CACCGCAGTA GACCCATCTA CGTCTCTACA GTCCGGCCGC AGTCAAAGCA CATAGTAGTG 
ATTCTGGACC ACGGGGCTTC AGTCACAGAC ACTCAGCTTC AGATTGCCAA GGACGCTGCT 
CAGGTCATCC TCAGCGCCAT CGATGAACAT GACAAGATTT CTGTGTTAAC TGTGGCAGAT 
ACCGTCCGGA CTTGCTCACT AGACCAGTGC TATAAGACCT TCTTGTCTCC AGCCACCAGT 
GAGACAAAAA GGAAAATGTC CACCTTTGTT AGCAGCGTGA AGTCTTCAGA CAGTCCTACC 
CAGCACGCAG TGGGATTCCA AAAGGCATTT CAGCTGATTC GAAGTACAAA CAATAACACA 
AAGTTCCAAG CAAATACAGA CATGGTCATC ATTTACCTGT CAGCTGGCAT TACATCAAAG 
GACTCTTCGG AAGAAGATAA AAAAGCGACT CTCCAAGTCA TCAATGAAGA AAATAGCTTT 
CTAAACAACT CTGTAATGAT TCTCACCTAT GCCCTCATGA ACGATGGGGT GACTGGTTTG 
AAAGAGCTGG CTTTTCTGAG GGATCTAGCT GAACAGAATT CAGGGAAGTA CGGTGTGCCA 
GACCGGATGG CCTTGCCTGT GATTAAGGGC AGCATGATGG TGCTGAATCA GTTGAGCAAC 
CTGGAGACCA CAGTGGGCAG GTTCTACACA AACCTTCCCA ACCGGATGAT TGATGAAGCC 
GTCTTCAGCC TGCCCTTCTC TGATGAGATG GGAGATGGTT TGATAATGAC TGTGAGTAAA 
CCCTGTTATT TTGGAAACCT ACTTCTGGGA ATTGTAGGTG TGGACGTGAA TCTGGCTTAC 
ATTCTTGAAG ACGTGACGTA TTACCAAGAC TCTTTGGCTT CCTATACTTT TCTCATAGAC 
GACAAAGGAT ATACACTTAT GCACCCATCT CTTACCAGGC CATATTTATT GTCAGAGCCC 
CCACTTCATA CTGACATCAT ACATTATGAA AATATTCCAA AATTTGAATT AGTTCGGCAA 
AATATCCTAA GCCTCCCTCT GGGCAGCCAG ATTATCGCAG TCCCTGTGAA CTCATCCCTG 
TCTTGGCACA TAAACAAGCT GAGAGAAACT GGAAAGGAAG CCTACAATGT TAGCTATGCC 
TGGAAGATGG TACAAGACAC TTCCTTTATT CTGTGTATTG TGGTGATACA ACCAGAAATA 
CCTGTGAAAC AACTGAAGAA CCTCAACACT GTTCCCAGCA GCAAGCTGCT GTACCACCGG 
CTGGATCTCC TTGGCCAGCC CAGTGCTTGC CTCCACTTCA AACAGCTGGC AACCCTAGAA 
AGTCCCACCA TCATGCTGTC TGCTGGCAGC TTTTCCTCCC CCTATGAGCA CCTCAGCCAG 
CCAGAGACAA AGCGCATGGT AGAGCACTAC ACCGCCTATC TCAGCGACAA CACCCGCCTC 
ATTGCTAACC CGGGCCTCAA ATTCTCTGTC AGAAATGAAG TAATGGCTAC CAGCCACGTC 
ACAGATGAAT GGATGACACA AATGGAAATG AGTAGCCTGA ACACTTACAT TGTCCGCCGT 
TACATAGCAA CACCCAATGG CGTCCTCAGA ATTTATCCTG GTTCCCTCAT GGACAAAGCA 
TTTGATCCCA CTAGGAGACA ATGGTATCTC CATGCAGTAG CTAATCCAGG GTTGATTTCT 
TTGACTGGTC CTTACTTAGA TGTTGGAGGA GCTGGTTATG TTGTGACAAT CAGTCACACA 
ATTCATTCAT CCAGTACACA GCTGTCTTCT GGGCACACTG TGGCTGTGAT GGGCATTGAC 
TTCACACTCA GATACTTCTA CAAAGTTCTG ATGGACCTAT TACCTGTCTG TAACCAAGAT 
GGTGGCAACA AAATAAGGTG CTTCATAATG GAGGACAGGG GTTATCTGGT GGCGCACCCG 
ACTCTCATCG ACCCCAAAGG ACATGCACCT GTGGAGCAGC AGCACATCAC CCACAAGGAG 
CCCCTGGTAG CAAATGATAT CCTCAACCAC CCCAACTTTG TAAAGAAAAA CCTGTGCAAC 
AGCTTCAGTG ACAGAACGGT CCAGAGGTTT TATAAATTCA ACACCAGCCT TGCGGGGGAT 
TTGACGAACC TTGTGCATGG CAGCCACTGT TCCAAATACA GATTAGCAAG GATCCCAGGA 
ACCAACGCGT TTGTTGGCAT TGTCAACGAA ACCTGCGACT CTCTTGCCTT CTGTGCCTGC 
AGCATGGTGG ACCGACTCTG TCTCAACTGT CACCGAATGG AACAAAATGA ATGTGAATGT 
CCTTGTGAGT GCCCTCTAGA GGTCAATGAG TGCACTGGCA ACCTCACCAA TGCAGAGAAC 
CGAAACCCCA GCTGCGAGGT CCACCAGGAG CCGGTGACAT ACACAGCTAT TGACCCTGGC 
CTGCAAGATG CTCTTCACCA GTGTGTCAAC AGCAGGTGCA GTCAGAGGCT GGAAAGTGGG 
GACTGTTTTG GGGTGCTGGA TTGTGAATGG TGCATGGTGG ACAGTGATGG AAAGACTCAC 
CTGGACAAAC CCTACTGTGC CCCCCAGAAA GAATGCTTCG GGGGGATTGT GGGAGCCAAA 
AGTCCCTACG TTGATGACAT GGGAGCAATA GGTGATGAGG TGATCACATT AAACATGATT 
AAAAGCGCCC CTGTGGGTCC TGTGGCTGGA GGGATCATGG GATGCATCAT GGTCTTGGTC 
CTGGCGGTGT ATGCCTACCG CCACCAGATT CATCGCCGGA GCCATCAGCA TATGTCTCCT 
CTTGCTGCCC AAGAAATGTC AGTGCGTATG TCCAACCTGG AGAATGACAG AGATGAAAGG 
GACGACGACA GCCACGAAGA CAGAGGCATC ATCAGCAACA CTCGGTTTAT AGCTGCGGTC 
ATCGAACGAC ATGCACACAG TCCAGAAAGA AGGCGCCGCT ACTGGGGTCG ATCAGGAACA 
GAAAGTGATC ATGGTTACAG CACCATGAGC CCACAGGAGG ACAGTGAAAA TCCTCCATGC 
AACAATGACC CCTTGTCAGC CGGGGTCGAT GTGGGAAACC ATGATGAGGA CTTAGACCTG 
GATACCCCCC CTCAGACTGC TGCCCTACTA AGTCACAAGT TCCACCACTA CCGGTCACAC 
CACCCTACAC TTCATCATAG CCACCACTTA CAGGCGGCCG TCACGGTACA CACTGTCGAT 
GCAGAATGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_065976.2
CDS106..3777
Translation

Target ORF information:

RefSeq Version NM_020925.2
Organism Homo sapiens(human)
Definition Homo sapiens cache domain containing 1 (CACHD1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_020925.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGCGGAGGC TGGCGGCCGA GGAGCTGGGG GTCGTCACCA TGCAGCGGAT ATTCAACTCC 
TTTGTTTACA CTGAGAAAAT CTCAAATGGA GAAAGTGAAG TACAGCAGCT AGCCAAAAAA 
ATCCGAGAGA AGTTCAACCG TTACTTGGAT GTGGTCAATC GGAACAAGCA AGTTGTAGAA 
GCATCCTATA CGGCTCACCT AACCTCTCCC CTAACTGCAA TTCAAGACTG CTGTACTATC 
CCACCTTCCA TGATGGAATT CGATGGGAAC TTTAATACCA ATGTGTCTAG AACAATTAGT 
TGTGATCGAC TTTCTACTAC TGTTAATAGC CGGGCCTTCA ATCCAGGACG AGACTTAAAT 
TCAGTTCTTG CAGACAACCT GAAATCCAAC CCTGGAATTA AGTGGCAATA TTTCAGTTCA 
GAAGAAGGAA TTTTCACTGT TTTCCCAGCA CACAAGTTCC GGTGTAAGGG CAGCTACGAA 
CACCGCAGTA GACCCATCTA CGTCTCTACA GTCCGGCCGC AGTCAAAGCA CATAGTAGTG 
ATTCTGGACC ACGGGGCTTC AGTCACAGAC ACTCAGCTTC AGATTGCCAA GGACGCTGCT 
CAGGTCATCC TCAGCGCCAT CGATGAACAT GACAAGATTT CTGTGTTAAC TGTGGCAGAT 
ACCGTCCGGA CTTGCTCACT AGACCAGTGC TATAAGACCT TCTTGTCTCC AGCCACCAGT 
GAGACAAAAA GGAAAATGTC CACCTTTGTT AGCAGCGTGA AGTCTTCAGA CAGTCCTACC 
CAGCACGCAG TGGGATTCCA AAAGGCATTT CAGCTGATTC GAAGTACAAA CAATAACACA 
AAGTTCCAAG CAAATACAGA CATGGTCATC ATTTACCTGT CAGCTGGCAT TACATCAAAG 
GACTCTTCGG AAGAAGATAA AAAAGCGACT CTCCAAGTCA TCAATGAAGA AAATAGCTTT 
CTAAACAACT CTGTAATGAT TCTCACCTAT GCCCTCATGA ACGATGGGGT GACTGGTTTG 
AAAGAGCTGG CTTTTCTGAG GGATCTAGCT GAACAGAATT CAGGGAAGTA CGGTGTGCCA 
GACCGGATGG CCTTGCCTGT GATTAAGGGC AGCATGATGG TGCTGAATCA GTTGAGCAAC 
CTGGAGACCA CAGTGGGCAG GTTCTACACA AACCTTCCCA ACCGGATGAT TGATGAAGCC 
GTCTTCAGCC TGCCCTTCTC TGATGAGATG GGAGATGGTT TGATAATGAC TGTGAGTAAA 
CCCTGTTATT TTGGAAACCT ACTTCTGGGA ATTGTAGGTG TGGACGTGAA TCTGGCTTAC 
ATTCTTGAAG ACGTGACGTA TTACCAAGAC TCTTTGGCTT CCTATACTTT TCTCATAGAC 
GACAAAGGAT ATACACTTAT GCACCCATCT CTTACCAGGC CATATTTATT GTCAGAGCCC 
CCACTTCATA CTGACATCAT ACATTATGAA AATATTCCAA AATTTGAATT AGTTCGGCAA 
AATATCCTAA GCCTCCCTCT GGGCAGCCAG ATTATCGCAG TCCCTGTGAA CTCATCCCTG 
TCTTGGCACA TAAACAAGCT GAGAGAAACT GGAAAGGAAG CCTACAATGT TAGCTATGCC 
TGGAAGATGG TACAAGACAC TTCCTTTATT CTGTGTATTG TGGTGATACA ACCAGAAATA 
CCTGTGAAAC AACTGAAGAA CCTCAACACT GTTCCCAGCA GCAAGCTGCT GTACCACCGG 
CTGGATCTCC TTGGCCAGCC CAGTGCTTGC CTCCACTTCA AACAGCTGGC AACCCTAGAA 
AGTCCCACCA TCATGCTGTC TGCTGGCAGC TTTTCCTCCC CCTATGAGCA CCTCAGCCAG 
CCAGAGACAA AGCGCATGGT AGAGCACTAC ACCGCCTATC TCAGCGACAA CACCCGCCTC 
ATTGCTAACC CGGGCCTCAA ATTCTCTGTC AGAAATGAAG TAATGGCTAC CAGCCACGTC 
ACAGATGAAT GGATGACACA AATGGAAATG AGTAGCCTGA ACACTTACAT TGTCCGCCGT 
TACATAGCAA CACCCAATGG CGTCCTCAGA ATTTATCCTG GTTCCCTCAT GGACAAAGCA 
TTTGATCCCA CTAGGAGACA ATGGTATCTC CATGCAGTAG CTAATCCAGG GTTGATTTCT 
TTGACTGGTC CTTACTTAGA TGTTGGAGGA GCTGGTTATG TTGTGACAAT CAGTCACACA 
ATTCATTCAT CCAGTACACA GCTGTCTTCT GGGCACACTG TGGCTGTGAT GGGCATTGAC 
TTCACACTCA GATACTTCTA CAAAGTTCTG ATGGACCTAT TACCTGTCTG TAACCAAGAT 
GGTGGCAACA AAATAAGGTG CTTCATAATG GAGGACAGGG GTTATCTGGT GGCGCACCCG 
ACTCTCATCG ACCCCAAAGG ACATGCACCT GTGGAGCAGC AGCACATCAC CCACAAGGAG 
CCCCTGGTAG CAAATGATAT CCTCAACCAC CCCAACTTTG TAAAGAAAAA CCTGTGCAAC 
AGCTTCAGTG ACAGAACGGT CCAGAGGTTT TATAAATTCA ACACCAGCCT TGCGGGGGAT 
TTGACGAACC TTGTGCATGG CAGCCACTGT TCCAAATACA GATTAGCAAG GATCCCAGGA 
ACCAACGCGT TTGTTGGCAT TGTCAACGAA ACCTGCGACT CTCTTGCCTT CTGTGCCTGC 
AGCATGGTGG ACCGACTCTG TCTCAACTGT CACCGAATGG AACAAAATGA ATGTGAATGT 
CCTTGTGAGT GCCCTCTAGA GGTCAATGAG TGCACTGGCA ACCTCACCAA TGCAGAGAAC 
CGAAACCCCA GCTGCGAGGT CCACCAGGAG CCGGTGACAT ACACAGCTAT TGACCCTGGC 
CTGCAAGATG CTCTTCACCA GTGTGTCAAC AGCAGGTGCA GTCAGAGGCT GGAAAGTGGG 
GACTGTTTTG GGGTGCTGGA TTGTGAATGG TGCATGGTGG ACAGTGATGG AAAGACTCAC 
CTGGACAAAC CCTACTGTGC CCCCCAGAAA GAATGCTTCG GGGGGATTGT GGGAGCCAAA 
AGTCCCTACG TTGATGACAT GGGAGCAATA GGTGATGAGG TGATCACATT AAACATGATT 
AAAAGCGCCC CTGTGGGTCC TGTGGCTGGA GGGATCATGG GATGCATCAT GGTCTTGGTC 
CTGGCGGTGT ATGCCTACCG CCACCAGATT CATCGCCGGA GCCATCAGCA TATGTCTCCT 
CTTGCTGCCC AAGAAATGTC AGTGCGTATG TCCAACCTGG AGAATGACAG AGATGAAAGG 
GACGACGACA GCCACGAAGA CAGAGGCATC ATCAGCAACA CTCGGTTTAT AGCTGCGGTC 
ATCGAACGAC ATGCACACAG TCCAGAAAGA AGGCGCCGCT ACTGGGGTCG ATCAGGAACA 
GAAAGTGATC ATGGTTACAG CACCATGAGC CCACAGGAGG ACAGTGAAAA TCCTCCATGC 
AACAATGACC CCTTGTCAGC CGGGGTCGAT GTGGGAAACC ATGATGAGGA CTTAGACCTG 
GATACCCCCC CTCAGACTGC TGCCCTACTA AGTCACAAGT TCCACCACTA CCGGTCACAC 
CACCCTACAC TTCATCATAG CCACCACTTA CAGGCGGCCG TCACGGTACA CACTGTCGAT 
GCAGAATGCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu70844
Clone ID Related Accession (Same CDS sequence) XM_011541862.1
Accession Version XM_011541862.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product VWFA and cache domain-containing protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGCGGAT ATTCAACTCC TTTGTTTACA CTGAGAAAAT CTCAAATGGA GAAAGTGAAG 
TACAGCAGAT TCGATGGGAA CTTTAATACC AATGTGTCTA GAACAATTAG TTGTGATCGA 
CTTTCTACTA CTGTTAATAG CCGGGCCTTC AATCCAGGAC GAGACTTAAA TTCAGTTCTT 
GCAGACAACC TGAAATCCAA CCCTGGAATT AAGTGGCAAT ATTTCAGTTC AGAAGAAGGA 
ATTTTCACTG TTTTCCCAGC ACACAAGTTC CGGTGTAAGG GCAGCTACGA ACACCGCAGT 
AGACCCATCT ACGTCTCTAC AGTCCGGCCG CAGTCAAAGC ACATAGTAGT GATTCTGGAC 
CACGGGGCTT CAGTCACAGA CACTCAGCTT CAGATTGCCA AGGACGCTGC TCAGGTCATC 
CTCAGCGCCA TCGATGAACA TGACAAGATT TCTGTGTTAA CTGTGGCAGA TACCGTCCGG 
ACTTGCTCAC TAGACCAGTG CTATAAGACC TTCTTGTCTC CAGCCACCAG TGAGACAAAA 
AGGAAAATGT CCACCTTTGT TAGCAGCGTG AAGTCTTCAG ACAGTCCTAC CCAGCACGCA 
GTGGGATTCC AAAAGGCATT TCAGCTGATT CGAAGTACAA ACAATAACAC AAAGTTCCAA 
GCAAATACAG ACATGGTCAT CATTTACCTG TCAGCTGGCA TTACATCAAA GGACTCTTCG 
GAAGAAGATA AAAAAGCGAC TCTCCAAGTC ATCAATGAAG AAAATAGCTT TCTAAACAAC 
TCTGTAATGA TTCTCACCTA TGCCCTCATG AACGATGGGG TGACTGGTTT GAAAGAGCTG 
GCTTTTCTGA GGGATCTAGC TGAACAGAAT TCAGGGAAGT ACGGTGTGCC AGACCGGATG 
GCCTTGCCTG TGATTAAGGG CAGCATGATG GTGCTGAATC AGTTGAGCAA CCTGGAGACC 
ACAGTGGGCA GGTTCTACAC AAACCTTCCC AACCGGATGA TTGATGAAGC CGTCTTCAGC 
CTGCCCTTCT CTGATGAGAT GGGAGATGGT TTGATAATGA CTGTGAGTAA ACCCTGTTAT 
TTTGGAAACC TACTTCTGGG AATTGTAGGT GTGGACGTGA ATCTGGCTTA CATTCTTGAA 
GACGTGACGT ATTACCAAGA CTCTTTGGCT TCCTATACTT TTCTCATAGA CGACAAAGGA 
TATACACTTA TGCACCCATC TCTTACCAGG CCATATTTAT TGTCAGAGCC CCCACTTCAT 
ACTGACATCA TACATTATGA AAATATTCCA AAATTTGAAT TAGTTCGGCA AAATATCCTA 
AGCCTCCCTC TGGGCAGCCA GATTATCGCA GTCCCTGTGA ACTCATCCCT GTCTTGGCAC 
ATAAACAAGC TGAGAGAAAC TGGAAAGGAA GCCTACAATG TTAGCTATGC CTGGAAGATG 
GTACAAGACA CTTCCTTTAT TCTGTGTATT GTGGTGATAC AACCAGAAAT ACCTGTGAAA 
CAACTGAAGA ACCTCAACAC TGTTCCCAGC AGCAAGCTGC TGTACCACCG GCTGGATCTC 
CTTGGCCAGC CCAGTGCTTG CCTCCACTTC AAACAGCTGG CAACCCTAGA AAGTCCCACC 
ATCATGCTGT CTGCTGGCAG CTTTTCCTCC CCCTATGAGC ACCTCAGCCA GCCAGAGACA 
AAGCGCATGG TAGAGCACTA CACCGCCTAT CTCAGCGACA ACACCCGCCT CATTGCTAAC 
CCGGGCCTCA AATTCTCTGT CAGAAATGAA GTAATGGCTA CCAGCCACGT CACAGATGAA 
TGGATGACAC AAATGGAAAT GAGTAGCCTG AACACTTACA TTGTCCGCCG TTACATAGCA 
ACACCCAATG GCGTCCTCAG AATTTATCCT GGTTCCCTCA TGGACAAAGC ATTTGATCCC 
ACTAGGAGAC AATGGTATCT CCATGCAGTA GCTAATCCAG GGTTGATTTC TTTGACTGGT 
CCTTACTTAG ATGTTGGAGG AGCTGGTTAT GTTGTGACAA TCAGTCACAC AATTCATTCA 
TCCAGTACAC AGCTGTCTTC TGGGCACACT GTGGCTGTGA TGGGCATTGA CTTCACACTC 
AGATACTTCT ACAAAGTTCT GATGGACCTA TTACCTGTCT GTAACCAAGA TGGTGGCAAC 
AAAATAAGGT GCTTCATAAT GGAGGACAGG GGTTATCTGG TGGCGCACCC GACTCTCATC 
GACCCCAAAG GACATGCACC TGTGGAGCAG CAGCACATCA CCCACAAGGA GCCCCTGGTA 
GCAAATGATA TCCTCAACCA CCCCAACTTT GTAAAGAAAA ACCTGTGCAA CAGCTTCAGT 
GACAGAACGG TCCAGAGGTT TTATAAATTC AACACCAGCC TTGCGGGGGA TTTGACGAAC 
CTTGTGCATG GCAGCCACTG TTCCAAATAC AGATTAGCAA GGATCCCAGG AACCAACGCG 
TTTGTTGGCA TTGTCAACGA AACCTGCGAC TCTCTTGCCT TCTGTGCCTG CAGCATGGTG 
GACCGACTCT GTCTCAACTG TCACCGAATG GAACAAAATG AATGTGAATG TCCTTGTGAG 
TGCCCTCTAG AGGTCAATGA GTGCACTGGC AACCTCACCA ATGCAGAGAA CCGAAACCCC 
AGCTGCGAGG TCCACCAGGA GCCGGTGACA TACACAGCTA TTGACCCTGG CCTGCAAGAT 
GCTCTTCACC AGTGTGTCAA CAGCAGGTGC AGTCAGAGGC TGGAAAGTGG GGACTGTTTT 
GGGGTGCTGG ATTGTGAATG GTGCATGGTG GACAGTGATG GAAAGACTCA CCTGGACAAA 
CCCTACTGTG CCCCCCAGAA AGAATGCTTC GGGGGGATTG TGGGAGCCAA AAGTCCCTAC 
GTTGATGACA TGGGAGCAAT AGGTGATGAG GTGATCACAT TAAACATGAT TAAAAGCGCC 
CCTGTGGGTC CTGTGGCTGG AGGGATCATG GGATGCATCA TGGTCTTGGT CCTGGCGGTG 
TATGCCTACC GCCACCAGAT TCATCGCCGG AGCCATCAGC ATATGTCTCC TCTTGCTGCC 
CAAGAAATGT CAGTGCGTAT GTCCAACCTG GAGAATGACA GAGATGAAAG GGACGACGAC 
AGCCACGAAG ACAGAGGCAT CATCAGCAAC ACTCGGTTTA TAGCTGCGGT CATCGAACGA 
CATGCACACA GTCCAGAAAG AAGGCGCCGC TACTGGGGTC GATCAGGAAC AGAAAGTGAT 
CATGGTTACA GCACCATGAG CCCACAGGAG GACAGTGAAA ATCCTCCATG CAACAATGAC 
CCCTTGTCAG CCGGGGTCGA TGTGGGAAAC CATGATGAGG ACTTAGACCT GGATACCCCC 
CCTCAGACTG CTGCCCTACT AAGTCACAAG TTCCACCACT ACCGGTCACA CCACCCTACA 
CTTCATCATA GCCACCACTT ACAGGCGGCC GTCACGGTAC ACACTGTCGA TGCAGAATGC 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011540164.1
CDS570..4052
Translation

Target ORF information:

RefSeq Version XM_011541862.1
Organism Homo sapiens(human)
Definition Homo sapiens cache domain containing 1 (CACHD1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011541862.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGAGCGGAT ATTCAACTCC TTTGTTTACA CTGAGAAAAT CTCAAATGGA GAAAGTGAAG 
TACAGCAGAT TCGATGGGAA CTTTAATACC AATGTGTCTA GAACAATTAG TTGTGATCGA 
CTTTCTACTA CTGTTAATAG CCGGGCCTTC AATCCAGGAC GAGACTTAAA TTCAGTTCTT 
GCAGACAACC TGAAATCCAA CCCTGGAATT AAGTGGCAAT ATTTCAGTTC AGAAGAAGGA 
ATTTTCACTG TTTTCCCAGC ACACAAGTTC CGGTGTAAGG GCAGCTACGA ACACCGCAGT 
AGACCCATCT ACGTCTCTAC AGTCCGGCCG CAGTCAAAGC ACATAGTAGT GATTCTGGAC 
CACGGGGCTT CAGTCACAGA CACTCAGCTT CAGATTGCCA AGGACGCTGC TCAGGTCATC 
CTCAGCGCCA TCGATGAACA TGACAAGATT TCTGTGTTAA CTGTGGCAGA TACCGTCCGG 
ACTTGCTCAC TAGACCAGTG CTATAAGACC TTCTTGTCTC CAGCCACCAG TGAGACAAAA 
AGGAAAATGT CCACCTTTGT TAGCAGCGTG AAGTCTTCAG ACAGTCCTAC CCAGCACGCA 
GTGGGATTCC AAAAGGCATT TCAGCTGATT CGAAGTACAA ACAATAACAC AAAGTTCCAA 
GCAAATACAG ACATGGTCAT CATTTACCTG TCAGCTGGCA TTACATCAAA GGACTCTTCG 
GAAGAAGATA AAAAAGCGAC TCTCCAAGTC ATCAATGAAG AAAATAGCTT TCTAAACAAC 
TCTGTAATGA TTCTCACCTA TGCCCTCATG AACGATGGGG TGACTGGTTT GAAAGAGCTG 
GCTTTTCTGA GGGATCTAGC TGAACAGAAT TCAGGGAAGT ACGGTGTGCC AGACCGGATG 
GCCTTGCCTG TGATTAAGGG CAGCATGATG GTGCTGAATC AGTTGAGCAA CCTGGAGACC 
ACAGTGGGCA GGTTCTACAC AAACCTTCCC AACCGGATGA TTGATGAAGC CGTCTTCAGC 
CTGCCCTTCT CTGATGAGAT GGGAGATGGT TTGATAATGA CTGTGAGTAA ACCCTGTTAT 
TTTGGAAACC TACTTCTGGG AATTGTAGGT GTGGACGTGA ATCTGGCTTA CATTCTTGAA 
GACGTGACGT ATTACCAAGA CTCTTTGGCT TCCTATACTT TTCTCATAGA CGACAAAGGA 
TATACACTTA TGCACCCATC TCTTACCAGG CCATATTTAT TGTCAGAGCC CCCACTTCAT 
ACTGACATCA TACATTATGA AAATATTCCA AAATTTGAAT TAGTTCGGCA AAATATCCTA 
AGCCTCCCTC TGGGCAGCCA GATTATCGCA GTCCCTGTGA ACTCATCCCT GTCTTGGCAC 
ATAAACAAGC TGAGAGAAAC TGGAAAGGAA GCCTACAATG TTAGCTATGC CTGGAAGATG 
GTACAAGACA CTTCCTTTAT TCTGTGTATT GTGGTGATAC AACCAGAAAT ACCTGTGAAA 
CAACTGAAGA ACCTCAACAC TGTTCCCAGC AGCAAGCTGC TGTACCACCG GCTGGATCTC 
CTTGGCCAGC CCAGTGCTTG CCTCCACTTC AAACAGCTGG CAACCCTAGA AAGTCCCACC 
ATCATGCTGT CTGCTGGCAG CTTTTCCTCC CCCTATGAGC ACCTCAGCCA GCCAGAGACA 
AAGCGCATGG TAGAGCACTA CACCGCCTAT CTCAGCGACA ACACCCGCCT CATTGCTAAC 
CCGGGCCTCA AATTCTCTGT CAGAAATGAA GTAATGGCTA CCAGCCACGT CACAGATGAA 
TGGATGACAC AAATGGAAAT GAGTAGCCTG AACACTTACA TTGTCCGCCG TTACATAGCA 
ACACCCAATG GCGTCCTCAG AATTTATCCT GGTTCCCTCA TGGACAAAGC ATTTGATCCC 
ACTAGGAGAC AATGGTATCT CCATGCAGTA GCTAATCCAG GGTTGATTTC TTTGACTGGT 
CCTTACTTAG ATGTTGGAGG AGCTGGTTAT GTTGTGACAA TCAGTCACAC AATTCATTCA 
TCCAGTACAC AGCTGTCTTC TGGGCACACT GTGGCTGTGA TGGGCATTGA CTTCACACTC 
AGATACTTCT ACAAAGTTCT GATGGACCTA TTACCTGTCT GTAACCAAGA TGGTGGCAAC 
AAAATAAGGT GCTTCATAAT GGAGGACAGG GGTTATCTGG TGGCGCACCC GACTCTCATC 
GACCCCAAAG GACATGCACC TGTGGAGCAG CAGCACATCA CCCACAAGGA GCCCCTGGTA 
GCAAATGATA TCCTCAACCA CCCCAACTTT GTAAAGAAAA ACCTGTGCAA CAGCTTCAGT 
GACAGAACGG TCCAGAGGTT TTATAAATTC AACACCAGCC TTGCGGGGGA TTTGACGAAC 
CTTGTGCATG GCAGCCACTG TTCCAAATAC AGATTAGCAA GGATCCCAGG AACCAACGCG 
TTTGTTGGCA TTGTCAACGA AACCTGCGAC TCTCTTGCCT TCTGTGCCTG CAGCATGGTG 
GACCGACTCT GTCTCAACTG TCACCGAATG GAACAAAATG AATGTGAATG TCCTTGTGAG 
TGCCCTCTAG AGGTCAATGA GTGCACTGGC AACCTCACCA ATGCAGAGAA CCGAAACCCC 
AGCTGCGAGG TCCACCAGGA GCCGGTGACA TACACAGCTA TTGACCCTGG CCTGCAAGAT 
GCTCTTCACC AGTGTGTCAA CAGCAGGTGC AGTCAGAGGC TGGAAAGTGG GGACTGTTTT 
GGGGTGCTGG ATTGTGAATG GTGCATGGTG GACAGTGATG GAAAGACTCA CCTGGACAAA 
CCCTACTGTG CCCCCCAGAA AGAATGCTTC GGGGGGATTG TGGGAGCCAA AAGTCCCTAC 
GTTGATGACA TGGGAGCAAT AGGTGATGAG GTGATCACAT TAAACATGAT TAAAAGCGCC 
CCTGTGGGTC CTGTGGCTGG AGGGATCATG GGATGCATCA TGGTCTTGGT CCTGGCGGTG 
TATGCCTACC GCCACCAGAT TCATCGCCGG AGCCATCAGC ATATGTCTCC TCTTGCTGCC 
CAAGAAATGT CAGTGCGTAT GTCCAACCTG GAGAATGACA GAGATGAAAG GGACGACGAC 
AGCCACGAAG ACAGAGGCAT CATCAGCAAC ACTCGGTTTA TAGCTGCGGT CATCGAACGA 
CATGCACACA GTCCAGAAAG AAGGCGCCGC TACTGGGGTC GATCAGGAAC AGAAAGTGAT 
CATGGTTACA GCACCATGAG CCCACAGGAG GACAGTGAAA ATCCTCCATG CAACAATGAC 
CCCTTGTCAG CCGGGGTCGA TGTGGGAAAC CATGATGAGG ACTTAGACCT GGATACCCCC 
CCTCAGACTG CTGCCCTACT AAGTCACAAG TTCCACCACT ACCGGTCACA CCACCCTACA 
CTTCATCATA GCCACCACTT ACAGGCGGCC GTCACGGTAC ACACTGTCGA TGCAGAATGC 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu70845
Clone ID Related Accession (Same CDS sequence) XM_011541863.1
Accession Version XM_011541863.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3423bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product VWFA and cache domain-containing protein 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGATGGAAT TCGATGGGAA CTTTAATACC AATGTGTCTA GAACAATTAG TTGTGATCGA 
CTTTCTACTA CTGTTAATAG CCGGGCCTTC AATCCAGGAC GAGACTTAAA TTCAGTTCTT 
GCAGACAACC TGAAATCCAA CCCTGGAATT AAGTGGCAAT ATTTCAGTTC AGAAGAAGGA 
ATTTTCACTG TTTTCCCAGC ACACAAGTTC CGGTGTAAGG GCAGCTACGA ACACCGCAGT 
AGACCCATCT ACGTCTCTAC AGTCCGGCCG CAGTCAAAGC ACATAGTAGT GATTCTGGAC 
CACGGGGCTT CAGTCACAGA CACTCAGCTT CAGATTGCCA AGGACGCTGC TCAGGTCATC 
CTCAGCGCCA TCGATGAACA TGACAAGATT TCTGTGTTAA CTGTGGCAGA TACCGTCCGG 
ACTTGCTCAC TAGACCAGTG CTATAAGACC TTCTTGTCTC CAGCCACCAG TGAGACAAAA 
AGGAAAATGT CCACCTTTGT TAGCAGCGTG AAGTCTTCAG ACAGTCCTAC CCAGCACGCA 
GTGGGATTCC AAAAGGCATT TCAGCTGATT CGAAGTACAA ACAATAACAC AAAGTTCCAA 
GCAAATACAG ACATGGTCAT CATTTACCTG TCAGCTGGCA TTACATCAAA GGACTCTTCG 
GAAGAAGATA AAAAAGCGAC TCTCCAAGTC ATCAATGAAG AAAATAGCTT TCTAAACAAC 
TCTGTAATGA TTCTCACCTA TGCCCTCATG AACGATGGGG TGACTGGTTT GAAAGAGCTG 
GCTTTTCTGA GGGATCTAGC TGAACAGAAT TCAGGGAAGT ACGGTGTGCC AGACCGGATG 
GCCTTGCCTG TGATTAAGGG CAGCATGATG GTGCTGAATC AGTTGAGCAA CCTGGAGACC 
ACAGTGGGCA GGTTCTACAC AAACCTTCCC AACCGGATGA TTGATGAAGC CGTCTTCAGC 
CTGCCCTTCT CTGATGAGAT GGGAGATGGT TTGATAATGA CTGTGAGTAA ACCCTGTTAT 
TTTGGAAACC TACTTCTGGG AATTGTAGGT GTGGACGTGA ATCTGGCTTA CATTCTTGAA 
GACGTGACGT ATTACCAAGA CTCTTTGGCT TCCTATACTT TTCTCATAGA CGACAAAGGA 
TATACACTTA TGCACCCATC TCTTACCAGG CCATATTTAT TGTCAGAGCC CCCACTTCAT 
ACTGACATCA TACATTATGA AAATATTCCA AAATTTGAAT TAGTTCGGCA AAATATCCTA 
AGCCTCCCTC TGGGCAGCCA GATTATCGCA GTCCCTGTGA ACTCATCCCT GTCTTGGCAC 
ATAAACAAGC TGAGAGAAAC TGGAAAGGAA GCCTACAATG TTAGCTATGC CTGGAAGATG 
GTACAAGACA CTTCCTTTAT TCTGTGTATT GTGGTGATAC AACCAGAAAT ACCTGTGAAA 
CAACTGAAGA ACCTCAACAC TGTTCCCAGC AGCAAGCTGC TGTACCACCG GCTGGATCTC 
CTTGGCCAGC CCAGTGCTTG CCTCCACTTC AAACAGCTGG CAACCCTAGA AAGTCCCACC 
ATCATGCTGT CTGCTGGCAG CTTTTCCTCC CCCTATGAGC ACCTCAGCCA GCCAGAGACA 
AAGCGCATGG TAGAGCACTA CACCGCCTAT CTCAGCGACA ACACCCGCCT CATTGCTAAC 
CCGGGCCTCA AATTCTCTGT CAGAAATGAA GTAATGGCTA CCAGCCACGT CACAGATGAA 
TGGATGACAC AAATGGAAAT GAGTAGCCTG AACACTTACA TTGTCCGCCG TTACATAGCA 
ACACCCAATG GCGTCCTCAG AATTTATCCT GGTTCCCTCA TGGACAAAGC ATTTGATCCC 
ACTAGGAGAC AATGGTATCT CCATGCAGTA GCTAATCCAG GGTTGATTTC TTTGACTGGT 
CCTTACTTAG ATGTTGGAGG AGCTGGTTAT GTTGTGACAA TCAGTCACAC AATTCATTCA 
TCCAGTACAC AGCTGTCTTC TGGGCACACT GTGGCTGTGA TGGGCATTGA CTTCACACTC 
AGATACTTCT ACAAAGTTCT GATGGACCTA TTACCTGTCT GTAACCAAGA TGGTGGCAAC 
AAAATAAGGT GCTTCATAAT GGAGGACAGG GGTTATCTGG TGGCGCACCC GACTCTCATC 
GACCCCAAAG GACATGCACC TGTGGAGCAG CAGCACATCA CCCACAAGGA GCCCCTGGTA 
GCAAATGATA TCCTCAACCA CCCCAACTTT GTAAAGAAAA ACCTGTGCAA CAGCTTCAGT 
GACAGAACGG TCCAGAGGTT TTATAAATTC AACACCAGCC TTGCGGGGGA TTTGACGAAC 
CTTGTGCATG GCAGCCACTG TTCCAAATAC AGATTAGCAA GGATCCCAGG AACCAACGCG 
TTTGTTGGCA TTGTCAACGA AACCTGCGAC TCTCTTGCCT TCTGTGCCTG CAGCATGGTG 
GACCGACTCT GTCTCAACTG TCACCGAATG GAACAAAATG AATGTGAATG TCCTTGTGAG 
TGCCCTCTAG AGGTCAATGA GTGCACTGGC AACCTCACCA ATGCAGAGAA CCGAAACCCC 
AGCTGCGAGG TCCACCAGGA GCCGGTGACA TACACAGCTA TTGACCCTGG CCTGCAAGAT 
GCTCTTCACC AGTGTGTCAA CAGCAGGTGC AGTCAGAGGC TGGAAAGTGG GGACTGTTTT 
GGGGTGCTGG ATTGTGAATG GTGCATGGTG GACAGTGATG GAAAGACTCA CCTGGACAAA 
CCCTACTGTG CCCCCCAGAA AGAATGCTTC GGGGGGATTG TGGGAGCCAA AAGTCCCTAC 
GTTGATGACA TGGGAGCAAT AGGTGATGAG GTGATCACAT TAAACATGAT TAAAAGCGCC 
CCTGTGGGTC CTGTGGCTGG AGGGATCATG GGATGCATCA TGGTCTTGGT CCTGGCGGTG 
TATGCCTACC GCCACCAGAT TCATCGCCGG AGCCATCAGC ATATGTCTCC TCTTGCTGCC 
CAAGAAATGT CAGTGCGTAT GTCCAACCTG GAGAATGACA GAGATGAAAG GGACGACGAC 
AGCCACGAAG ACAGAGGCAT CATCAGCAAC ACTCGGTTTA TAGCTGCGGT CATCGAACGA 
CATGCACACA GTCCAGAAAG AAGGCGCCGC TACTGGGGTC GATCAGGAAC AGAAAGTGAT 
CATGGTTACA GCACCATGAG CCCACAGGAG GACAGTGAAA ATCCTCCATG CAACAATGAC 
CCCTTGTCAG CCGGGGTCGA TGTGGGAAAC CATGATGAGG ACTTAGACCT GGATACCCCC 
CCTCAGACTG CTGCCCTACT AAGTCACAAG TTCCACCACT ACCGGTCACA CCACCCTACA 
CTTCATCATA GCCACCACTT ACAGGCGGCC GTCACGGTAC ACACTGTCGA TGCAGAATGC 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011540165.1
CDS779..4201
Translation

Target ORF information:

RefSeq Version XM_011541863.1
Organism Homo sapiens(human)
Definition Homo sapiens cache domain containing 1 (CACHD1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011541863.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGATGGAAT TCGATGGGAA CTTTAATACC AATGTGTCTA GAACAATTAG TTGTGATCGA 
CTTTCTACTA CTGTTAATAG CCGGGCCTTC AATCCAGGAC GAGACTTAAA TTCAGTTCTT 
GCAGACAACC TGAAATCCAA CCCTGGAATT AAGTGGCAAT ATTTCAGTTC AGAAGAAGGA 
ATTTTCACTG TTTTCCCAGC ACACAAGTTC CGGTGTAAGG GCAGCTACGA ACACCGCAGT 
AGACCCATCT ACGTCTCTAC AGTCCGGCCG CAGTCAAAGC ACATAGTAGT GATTCTGGAC 
CACGGGGCTT CAGTCACAGA CACTCAGCTT CAGATTGCCA AGGACGCTGC TCAGGTCATC 
CTCAGCGCCA TCGATGAACA TGACAAGATT TCTGTGTTAA CTGTGGCAGA TACCGTCCGG 
ACTTGCTCAC TAGACCAGTG CTATAAGACC TTCTTGTCTC CAGCCACCAG TGAGACAAAA 
AGGAAAATGT CCACCTTTGT TAGCAGCGTG AAGTCTTCAG ACAGTCCTAC CCAGCACGCA 
GTGGGATTCC AAAAGGCATT TCAGCTGATT CGAAGTACAA ACAATAACAC AAAGTTCCAA 
GCAAATACAG ACATGGTCAT CATTTACCTG TCAGCTGGCA TTACATCAAA GGACTCTTCG 
GAAGAAGATA AAAAAGCGAC TCTCCAAGTC ATCAATGAAG AAAATAGCTT TCTAAACAAC 
TCTGTAATGA TTCTCACCTA TGCCCTCATG AACGATGGGG TGACTGGTTT GAAAGAGCTG 
GCTTTTCTGA GGGATCTAGC TGAACAGAAT TCAGGGAAGT ACGGTGTGCC AGACCGGATG 
GCCTTGCCTG TGATTAAGGG CAGCATGATG GTGCTGAATC AGTTGAGCAA CCTGGAGACC 
ACAGTGGGCA GGTTCTACAC AAACCTTCCC AACCGGATGA TTGATGAAGC CGTCTTCAGC 
CTGCCCTTCT CTGATGAGAT GGGAGATGGT TTGATAATGA CTGTGAGTAA ACCCTGTTAT 
TTTGGAAACC TACTTCTGGG AATTGTAGGT GTGGACGTGA ATCTGGCTTA CATTCTTGAA 
GACGTGACGT ATTACCAAGA CTCTTTGGCT TCCTATACTT TTCTCATAGA CGACAAAGGA 
TATACACTTA TGCACCCATC TCTTACCAGG CCATATTTAT TGTCAGAGCC CCCACTTCAT 
ACTGACATCA TACATTATGA AAATATTCCA AAATTTGAAT TAGTTCGGCA AAATATCCTA 
AGCCTCCCTC TGGGCAGCCA GATTATCGCA GTCCCTGTGA ACTCATCCCT GTCTTGGCAC 
ATAAACAAGC TGAGAGAAAC TGGAAAGGAA GCCTACAATG TTAGCTATGC CTGGAAGATG 
GTACAAGACA CTTCCTTTAT TCTGTGTATT GTGGTGATAC AACCAGAAAT ACCTGTGAAA 
CAACTGAAGA ACCTCAACAC TGTTCCCAGC AGCAAGCTGC TGTACCACCG GCTGGATCTC 
CTTGGCCAGC CCAGTGCTTG CCTCCACTTC AAACAGCTGG CAACCCTAGA AAGTCCCACC 
ATCATGCTGT CTGCTGGCAG CTTTTCCTCC CCCTATGAGC ACCTCAGCCA GCCAGAGACA 
AAGCGCATGG TAGAGCACTA CACCGCCTAT CTCAGCGACA ACACCCGCCT CATTGCTAAC 
CCGGGCCTCA AATTCTCTGT CAGAAATGAA GTAATGGCTA CCAGCCACGT CACAGATGAA 
TGGATGACAC AAATGGAAAT GAGTAGCCTG AACACTTACA TTGTCCGCCG TTACATAGCA 
ACACCCAATG GCGTCCTCAG AATTTATCCT GGTTCCCTCA TGGACAAAGC ATTTGATCCC 
ACTAGGAGAC AATGGTATCT CCATGCAGTA GCTAATCCAG GGTTGATTTC TTTGACTGGT 
CCTTACTTAG ATGTTGGAGG AGCTGGTTAT GTTGTGACAA TCAGTCACAC AATTCATTCA 
TCCAGTACAC AGCTGTCTTC TGGGCACACT GTGGCTGTGA TGGGCATTGA CTTCACACTC 
AGATACTTCT ACAAAGTTCT GATGGACCTA TTACCTGTCT GTAACCAAGA TGGTGGCAAC 
AAAATAAGGT GCTTCATAAT GGAGGACAGG GGTTATCTGG TGGCGCACCC GACTCTCATC 
GACCCCAAAG GACATGCACC TGTGGAGCAG CAGCACATCA CCCACAAGGA GCCCCTGGTA 
GCAAATGATA TCCTCAACCA CCCCAACTTT GTAAAGAAAA ACCTGTGCAA CAGCTTCAGT 
GACAGAACGG TCCAGAGGTT TTATAAATTC AACACCAGCC TTGCGGGGGA TTTGACGAAC 
CTTGTGCATG GCAGCCACTG TTCCAAATAC AGATTAGCAA GGATCCCAGG AACCAACGCG 
TTTGTTGGCA TTGTCAACGA AACCTGCGAC TCTCTTGCCT TCTGTGCCTG CAGCATGGTG 
GACCGACTCT GTCTCAACTG TCACCGAATG GAACAAAATG AATGTGAATG TCCTTGTGAG 
TGCCCTCTAG AGGTCAATGA GTGCACTGGC AACCTCACCA ATGCAGAGAA CCGAAACCCC 
AGCTGCGAGG TCCACCAGGA GCCGGTGACA TACACAGCTA TTGACCCTGG CCTGCAAGAT 
GCTCTTCACC AGTGTGTCAA CAGCAGGTGC AGTCAGAGGC TGGAAAGTGG GGACTGTTTT 
GGGGTGCTGG ATTGTGAATG GTGCATGGTG GACAGTGATG GAAAGACTCA CCTGGACAAA 
CCCTACTGTG CCCCCCAGAA AGAATGCTTC GGGGGGATTG TGGGAGCCAA AAGTCCCTAC 
GTTGATGACA TGGGAGCAAT AGGTGATGAG GTGATCACAT TAAACATGAT TAAAAGCGCC 
CCTGTGGGTC CTGTGGCTGG AGGGATCATG GGATGCATCA TGGTCTTGGT CCTGGCGGTG 
TATGCCTACC GCCACCAGAT TCATCGCCGG AGCCATCAGC ATATGTCTCC TCTTGCTGCC 
CAAGAAATGT CAGTGCGTAT GTCCAACCTG GAGAATGACA GAGATGAAAG GGACGACGAC 
AGCCACGAAG ACAGAGGCAT CATCAGCAAC ACTCGGTTTA TAGCTGCGGT CATCGAACGA 
CATGCACACA GTCCAGAAAG AAGGCGCCGC TACTGGGGTC GATCAGGAAC AGAAAGTGAT 
CATGGTTACA GCACCATGAG CCCACAGGAG GACAGTGAAA ATCCTCCATG CAACAATGAC 
CCCTTGTCAG CCGGGGTCGA TGTGGGAAAC CATGATGAGG ACTTAGACCT GGATACCCCC 
CCTCAGACTG CTGCCCTACT AAGTCACAAG TTCCACCACT ACCGGTCACA CCACCCTACA 
CTTCATCATA GCCACCACTT ACAGGCGGCC GTCACGGTAC ACACTGTCGA TGCAGAATGC 
TAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs.
Genome research12(11)1773-84(2002 Nov)
Nakayama M,Kikuno R,Ohara O