TLR4 cDNA ORF clone, Homo sapiens(human)

The following TLR4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLR4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26864 NM_138554.5
Latest version!
Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu27172 NM_138557.3
Latest version!
Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu27264 NM_003266.4
Latest version!
Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
View Old Accession Versions >>
OHu26864 NM_138554.4 Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu27172 NM_138557.2 Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu27264 NM_003266.3 Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26864
    Clone ID Related Accession (Same CDS sequence) NM_138554.4 , NM_138554.5
    Accession Version NM_138554.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-31
    Organism Homo sapiens(human)
    Product toll-like receptor 4 isoform A precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC117422.1, AK226155.1, BG622357.1 and DA218006.1. On Jan 21, 2012 this sequence version replaced NM_138554.3. Transcript Variant: This variant (1) encodes the longest isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.283490.1, SRR1803615.39895.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGATGTCTG CCTCGCGCCT GGCTGGGACT CTGATCCCAG CCATGGCCTT CCTCTCCTGC 
    GTGAGACCAG AAAGCTGGGA GCCCTGCGTG GAGGTGGTTC CTAATATTAC TTATCAATGC
    ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC
    CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG
    CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC
    CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA
    GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT
    CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT
    CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG
    GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_612564.1
    CDS299..2818
    Translation

    Target ORF information:

    RefSeq Version NM_138554.4
    Organism Homo sapiens(human)
    Definition Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_138554.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGATGTCTG CCTCGCGCCT GGCTGGGACT CTGATCCCAG CCATGGCCTT CCTCTCCTGC 
    GTGAGACCAG AAAGCTGGGA GCCCTGCGTG GAGGTGGTTC CTAATATTAC TTATCAATGC
    ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC
    CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG
    CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC
    CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA
    GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT
    CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT
    CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG
    GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26864
    Clone ID Related Accession (Same CDS sequence) NM_138554.4 , NM_138554.5
    Accession Version NM_138554.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2520bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product toll-like receptor 4 isoform A precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC117422.1, AK226155.1, BG622357.1, DA218006.1 and AL160272.16. On Nov 22, 2018 this sequence version replaced NM_138554.4. Transcript Variant: This variant (1) encodes the longest isoform (A). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U93091.1, SRR1803613.20869.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000355622.8/ ENSP00000363089.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGATGTCTG CCTCGCGCCT GGCTGGGACT CTGATCCCAG CCATGGCCTT CCTCTCCTGC 
    GTGAGACCAG AAAGCTGGGA GCCCTGCGTG GAGGTGGTTC CTAATATTAC TTATCAATGC
    ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC
    CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG
    CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC
    CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA
    GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT
    CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT
    CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG
    GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_612564.1
    CDS71..2590
    Translation

    Target ORF information:

    RefSeq Version NM_138554.5
    Organism Homo sapiens(human)
    Definition Homo sapiens toll like receptor 4 (TLR4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_138554.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGATGTCTG CCTCGCGCCT GGCTGGGACT CTGATCCCAG CCATGGCCTT CCTCTCCTGC 
    GTGAGACCAG AAAGCTGGGA GCCCTGCGTG GAGGTGGTTC CTAATATTAC TTATCAATGC
    ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC
    CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG
    CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC
    CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA
    GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT
    CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT
    CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG
    GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27172
    Clone ID Related Accession (Same CDS sequence) NM_138557.2 , NM_138557.3
    Accession Version NM_138557.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product toll-like receptor 4 isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC025294.1, AK226155.1, BG622357.1 and DA218006.1. On or before Jan 21, 2012 this sequence version replaced NR_024169.1, NM_138557.1. Transcript Variant: This variant (4) lacks an internal exon compared to transcript variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which results in an isoform (D) with a shorter N-terminus compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA187988.1, SRR1803614.111988.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_612567.1
    CDS732..2651
    Translation

    Target ORF information:

    RefSeq Version NM_138557.2
    Organism Homo sapiens(human)
    Definition Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_138557.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27172
    Clone ID Related Accession (Same CDS sequence) NM_138557.2 , NM_138557.3
    Accession Version NM_138557.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product toll-like receptor 4 isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC025294.1, AK226155.1, BG622357.1, DA218006.1 and AL160272.16. On May 31, 2019 this sequence version replaced NM_138557.2. Transcript Variant: This variant (4) lacks an internal exon compared to transcript variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which results in an isoform (D) with a shorter N-terminus compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.112207.1, SRR1660807.89521.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_612567.1
    CDS504..2423
    Translation

    Target ORF information:

    RefSeq Version NM_138557.3
    Organism Homo sapiens(human)
    Definition Homo sapiens toll like receptor 4 (TLR4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_138557.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA 
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27264
    Clone ID Related Accession (Same CDS sequence) NM_003266.4 , NM_003266.3
    Accession Version NM_003266.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product toll-like receptor 4 isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC143841.1, BC025294.1, AK226155.1, BG622357.1, DA218006.1 and AL160272.16. On Jun 1, 2019 this sequence version replaced NM_003266.3. Transcript Variant: This variant (3) contains an additional internal exon compared to transcript variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which results in an isoform (C) with a shorter N-terminus compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.93093.1, SRR1803611.103081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
    CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG
    CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC
    CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA
    GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT
    CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT
    CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG
    GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003257.1
    CDS311..2710
    Translation

    Target ORF information:

    RefSeq Version NM_003266.4
    Organism Homo sapiens(human)
    Definition Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003266.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
    CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG
    CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC
    CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA
    GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT
    CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT
    CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG
    GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27264
    Clone ID Related Accession (Same CDS sequence) NM_003266.4 , NM_003266.3
    Accession Version NM_003266.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product toll-like receptor 4 isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA187988.1, BC143841.1, BC025294.1, AK226155.1, BG622357.1 and DA218006.1. On or before Jan 21, 2012 this sequence version replaced NR_024168.1, NM_003266.2. Transcript Variant: This variant (3) contains an additional internal exon compared to transcript variant 1. This variant represents translation initiation at a downstream AUG compared to variant 1; the 5'-most initiation codon, as used in variant 1, is associated with a weak Kozak sequence and a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG, which results in an isoform (C) with a shorter N-terminus compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.103081.1, SRR1803612.123617.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
    CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG
    CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC
    CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA
    GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT
    CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT
    CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG
    GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003257.1
    CDS539..2938
    Translation

    Target ORF information:

    RefSeq Version NM_003266.3
    Organism Homo sapiens(human)
    Definition Homo sapiens toll like receptor 4 (TLR4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003266.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGCTGA ATTTCTACAA AATCCCCGAC AACCTCCCCT TCTCAACCAA GAACCTGGAC 
    CTGAGCTTTA ATCCCCTGAG GCATTTAGGC AGCTATAGCT TCTTCAGTTT CCCAGAACTG
    CAGGTGCTGG ATTTATCCAG GTGTGAAATC CAGACAATTG AAGATGGGGC ATATCAGAGC
    CTAAGCCACC TCTCTACCTT AATATTGACA GGAAACCCCA TCCAGAGTTT AGCCCTGGGA
    GCCTTTTCTG GACTATCAAG TTTACAGAAG CTGGTGGCTG TGGAGACAAA TCTAGCATCT
    CTAGAGAACT TCCCCATTGG ACATCTCAAA ACTTTGAAAG AACTTAATGT GGCTCACAAT
    CTTATCCAAT CTTTCAAATT ACCTGAGTAT TTTTCTAATC TGACCAATCT AGAGCACTTG
    GACCTTTCCA GCAACAAGAT TCAAAGTATT TATTGCACAG ACTTGCGGGT TCTACATCAA
    ATGCCCCTAC TCAATCTCTC TTTAGACCTG TCCCTGAACC CTATGAACTT TATCCAACCA
    GGTGCATTTA AAGAAATTAG GCTTCATAAG CTGACTTTAA GAAATAATTT TGATAGTTTA
    AATGTAATGA AAACTTGTAT TCAAGGTCTG GCTGGTTTAG AAGTCCATCG TTTGGTTCTG
    GGAGAATTTA GAAATGAAGG AAACTTGGAA AAGTTTGACA AATCTGCTCT AGAGGGCCTG
    TGCAATTTGA CCATTGAAGA ATTCCGATTA GCATACTTAG ACTACTACCT CGATGATATT
    ATTGACTTAT TTAATTGTTT GACAAATGTT TCTTCATTTT CCCTGGTGAG TGTGACTATT
    GAAAGGGTAA AAGACTTTTC TTATAATTTC GGATGGCAAC ATTTAGAATT AGTTAACTGT
    AAATTTGGAC AGTTTCCCAC ATTGAAACTC AAATCTCTCA AAAGGCTTAC TTTCACTTCC
    AACAAAGGTG GGAATGCTTT TTCAGAAGTT GATCTACCAA GCCTTGAGTT TCTAGATCTC
    AGTAGAAATG GCTTGAGTTT CAAAGGTTGC TGTTCTCAAA GTGATTTTGG GACAACCAGC
    CTAAAGTATT TAGATCTGAG CTTCAATGGT GTTATTACCA TGAGTTCAAA CTTCTTGGGC
    TTAGAACAAC TAGAACATCT GGATTTCCAG CATTCCAATT TGAAACAAAT GAGTGAGTTT
    TCAGTATTCC TATCACTCAG AAACCTCATT TACCTTGACA TTTCTCATAC TCACACCAGA
    GTTGCTTTCA ATGGCATCTT CAATGGCTTG TCCAGTCTCG AAGTCTTGAA AATGGCTGGC
    AATTCTTTCC AGGAAAACTT CCTTCCAGAT ATCTTCACAG AGCTGAGAAA CTTGACCTTC
    CTGGACCTCT CTCAGTGTCA ACTGGAGCAG TTGTCTCCAA CAGCATTTAA CTCACTCTCC
    AGTCTTCAGG TACTAAATAT GAGCCACAAC AACTTCTTTT CATTGGATAC GTTTCCTTAT
    AAGTGTCTGA ACTCCCTCCA GGTTCTTGAT TACAGTCTCA ATCACATAAT GACTTCCAAA
    AAACAGGAAC TACAGCATTT TCCAAGTAGT CTAGCTTTCT TAAATCTTAC TCAGAATGAC
    TTTGCTTGTA CTTGTGAACA CCAGAGTTTC CTGCAATGGA TCAAGGACCA GAGGCAGCTC
    TTGGTGGAAG TTGAACGAAT GGAATGTGCA ACACCTTCAG ATAAGCAGGG CATGCCTGTG
    CTGAGTTTGA ATATCACCTG TCAGATGAAT AAGACCATCA TTGGTGTGTC GGTCCTCAGT
    GTGCTTGTAG TATCTGTTGT AGCAGTTCTG GTCTATAAGT TCTATTTTCA CCTGATGCTT
    CTTGCTGGCT GCATAAAGTA TGGTAGAGGT GAAAACATCT ATGATGCCTT TGTTATCTAC
    TCAAGCCAGG ATGAGGACTG GGTAAGGAAT GAGCTAGTAA AGAATTTAGA AGAAGGGGTG
    CCTCCATTTC AGCTCTGCCT TCACTACAGA GACTTTATTC CCGGTGTGGC CATTGCTGCC
    AACATCATCC ATGAAGGTTT CCATAAAAGC CGAAAGGTGA TTGTTGTGGT GTCCCAGCAC
    TTCATCCAGA GCCGCTGGTG TATCTTTGAA TATGAGATTG CTCAGACCTG GCAGTTTCTG
    AGCAGTCGTG CTGGTATCAT CTTCATTGTC CTGCAGAAGG TGGAGAAGAC CCTGCTCAGG
    CAGCAGGTGG AGCTGTACCG CCTTCTCAGC AGGAACACTT ACCTGGAGTG GGAGGACAGT
    GTCCTGGGGC GGCACATCTT CTGGAGACGA CTCAGAAAAG CCCTGCTGGA TGGTAAATCA
    TGGAATCCAG AAGGAACAGT GGGTACAGGA TGCAATTGGC AGGAAGCAAC ATCTATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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