ITCH cDNA ORF clone, Homo sapiens(human)
Gene Symbol | ITCH |
Entrez Gene ID | 83737 |
Full Name | itchy E3 ubiquitin protein ligase |
Synonyms | ADMFD,AIF4,AIP4,NAPP1 |
General protein information |
|
Gene Type | protein-coding |
Organism | Homo sapiens(human) |
Genome | |
Summary | This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]. |
Disorder MIM: | |
Disorder Html: | Autoimmune disease, multisystem, with facial dysmorphism, 613385 (3) |
mRNA and Protein(s)
mRNA | Protein | Name |
---|---|---|
XM_011529078.1 | XP_011527380.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X1 |
NM_001257137.1 | NP_001244066.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 1 |
NM_001257138.2 | NP_001244067.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 3 |
NM_001324197.1 | NP_001311126.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 1 |
NM_001257137.3 | NP_001244066.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 1 |
NM_001324198.2 | NP_001311127.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 2 |
XM_024452005.1 | XP_024307773.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X1 |
XM_005260570.1 | XP_005260627.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X4 |
XM_017028092.2 | XP_016883581.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X6 |
NM_001257138.1 | NP_001244067.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 3 |
XM_011529077.1 | XP_011527379.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X1 |
NM_031483.6 | NP_113671.3 | E3 ubiquitin-protein ligase Itchy homolog isoform 2 |
NM_001324198.1 | NP_001311127.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 2 |
XM_017028092.1 | XP_016883581.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X6 |
NM_001324197.2 | NP_001311126.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 1 |
XM_017028090.1 | XP_016883579.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X2 |
XM_011529079.1 | XP_011527381.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X5 |
NM_001257137.2 | NP_001244066.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 1 |
XM_017028089.1 | XP_016883578.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X1 |
XM_017028091.1 | XP_016883580.1 | E3 ubiquitin-protein ligase Itchy homolog isoform X3 |
NM_031483.5 | NP_113671.3 | E3 ubiquitin-protein ligase Itchy homolog isoform 2 |
NM_001257138.3 | NP_001244067.1 | E3 ubiquitin-protein ligase Itchy homolog isoform 3 |
NM_031483.7 | NP_113671.3 | E3 ubiquitin-protein ligase Itchy homolog isoform 2 |

Homo sapiens (human) | ITCH | NP_113671.3 |
Canis lupus familiaris (dog) | ITCH | XP_851460.1 |
Mus musculus (house mouse) | Itch | NP_001230641.1 |
Rattus norvegicus (Norway rat) | Itch | NP_001005887.1 |
Bos taurus (cattle) | ITCH | NP_001075897.1 |
Gallus gallus (chicken) | ITCH | XP_417330.2 |
Danio rerio (zebrafish) | itch | XP_002667578.2 |
Danio rerio (zebrafish) | LOC100330031 | XP_003201702.2 |
LOC707640 | XP_002808005.1 | |
Xenopus tropicalis (tropical clawed frog) | itch | NP_001072737.1 |

The following ITCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OHu22317![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7 | |
Accession Version | NM_001324198.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-26 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 2 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL109923.29 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_001324198.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.17759.1, SRR1660807.164506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001311127.1 |
CDS | 197..2785 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22317![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7 | |
Accession Version | NM_031483.6 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-03-11 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 2 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, AK315212.1, AL109923.29, AA730741.1 and BG928040.1. On Apr 28, 2016 this sequence version replaced NM_031483.5. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Both variants 2 and 5 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.130987.1, SRR1660809.192411.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_113671.3 |
CDS | 214..2802 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22317![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7 | |
Accession Version | NM_001324198.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-03-11 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 2 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL705688.1, AL356299.16, AL109923.29 and BG928040.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.164506.1, SRR1803614.17759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001311127.1 |
CDS | 235..2823 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22317![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7 | |
Accession Version | NM_031483.5 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-07-02 | |
Organism | Homo sapiens(Human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 2 | |
Comment |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_113671.3 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22317![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_001324198.2 , NM_031483.6 , NM_001324198.1 , NM_031483.5 , NM_031483.7 | |
Accession Version | NM_031483.7 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-26 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 2 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, AK315212.1, AL109923.29, AA730741.1 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_031483.6. Transcript Variant: This variant (2) lacks an exon in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Both variants 2 and 5 encode the same isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.210367.1, SRR1803611.130987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_113671.3 |
CDS | 176..2764 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22837![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , NM_001257137.3 , XM_024452005.1 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1 | |
Accession Version | NM_001257137.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-07-02 | |
Organism | Homo sapiens(Human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 1 | |
Comment |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001244066.1 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22837![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , NM_001257137.3 , XM_024452005.1 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1 | |
Accession Version | NM_001324197.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-03-11 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL705688.1, AL356299.16, AL109923.29 and BG928040.1. On Apr 28, 2016 this sequence version replaced XM_011529077.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.88496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001311126.1 |
CDS | 235..2946 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22837![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , NM_001257137.3 , XM_024452005.1 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1 | |
Accession Version | NM_001257137.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-26 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, BC011571.1, AL109923.29, AA730741.1 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_001257137.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001244066.1 |
CDS | 176..2887 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22837![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , NM_001257137.3 , XM_024452005.1 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1 | |
Accession Version | NM_001324197.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-26 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL109923.29 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_001324197.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.88496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001311126.1 |
CDS | 197..2908 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22837![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , NM_001257137.3 , XM_024452005.1 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1 | |
Accession Version | NM_001257137.2 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-03-11 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, BC011571.1, AL109923.29, AA730741.1 and BG928040.1. On Apr 28, 2016 this sequence version replaced NM_001257137.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001244066.1 |
CDS | 214..2925 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu23120![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_001257138.2 , NM_001257138.1 , XM_017028091.1 , NM_001257138.3 | |
Accession Version | NM_001257138.2 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-03-11 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 3 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, AK304090.1, AF038564.1, AL109923.29, AA730741.1 and BG928040.1. On Apr 28, 2016 this sequence version replaced NM_001257138.1. Transcript Variant: This variant (3) lacks two internal exons and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001244067.1 |
CDS | 402..2660 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu23120![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_001257138.2 , NM_001257138.1 , XM_017028091.1 , NM_001257138.3 | |
Accession Version | NM_001257138.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-07-02 | |
Organism | Homo sapiens(Human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 3 | |
Comment |
1 | ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001244067.1 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu23120![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_001257138.2 , NM_001257138.1 , XM_017028091.1 , NM_001257138.3 | |
Accession Version | NM_001257138.3 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-08-26 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform 3 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL356299.16, AL705688.1, AK304090.1, AF038564.1, AL109923.29, AA730741.1 and BG928040.1. On Jun 2, 2019 this sequence version replaced NM_001257138.2. Transcript Variant: This variant (3) lacks two internal exons and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. |
1 | ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001244067.1 |
CDS | 364..2622 |
Translation |

Target ORF information:
Target ORF information:
|
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ORF Insert Sequence:
1 | ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22837![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , NM_001257137.3 , XM_024452005.1 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1 | |
Accession Version | XM_011529078.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-03-11 | |
Organism | Homo sapiens(Human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X1 | |
Comment |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011527380.1 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22837![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , NM_001257137.3 , XM_024452005.1 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1 | |
Accession Version | XM_024452005.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_024307773.1 |
CDS | 46..2757 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22837![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , NM_001257137.3 , XM_024452005.1 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1 | |
Accession Version | XM_011529077.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2015-03-11 | |
Organism | Homo sapiens(Human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X1 | |
Comment |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011527379.1 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu22837![]() |
|
Clone ID Related Accession (Same CDS sequence) | XM_011529078.1 , NM_001257137.1 , NM_001324197.1 , NM_001257137.3 , XM_024452005.1 , XM_011529077.1 , NM_001324197.2 , NM_001257137.2 , XM_017028089.1 | |
Accession Version | XM_017028089.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2712bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X1 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_016883578.1 |
CDS | 47..2758 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu23120![]() |
|
Clone ID Related Accession (Same CDS sequence) | NM_001257138.2 , NM_001257138.1 , XM_017028091.1 , NM_001257138.3 | |
Accession Version | XM_017028091.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X3 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_016883580.1 |
CDS | 415..2673 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGAAACTTG AAGAAGTAGT TGTGACTTTG CAGCTTGGAG GTGACAAAGA GCCAACAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu93131 | |
Clone ID Related Accession (Same CDS sequence) | XM_017028092.2 , XM_017028092.1 | |
Accession Version | XM_017028092.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X6 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_016883581.1 |
CDS | 176..1504 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu93131 | |
Clone ID Related Accession (Same CDS sequence) | XM_017028092.2 , XM_017028092.1 | |
Accession Version | XM_017028092.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2016-06-05 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X6 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_016883581.1 |
CDS | 176..1504 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu53122 | |
Clone ID Related Accession (Same CDS sequence) | XM_005260570.1 | |
Accession Version | XM_005260570.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X4 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTTCAGAA AAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_005260627.1 |
CDS | 640..2718 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTTCAGAA AAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu73156 | |
Clone ID Related Accession (Same CDS sequence) | XM_011529079.1 | |
Accession Version | XM_011529079.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X5 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGATATACA AAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_011527381.1 |
CDS | 660..2738 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGATATACA AAGTGAGCAC AAATGGATCA GATGACCCTG AAGATGCAGG AGCTGGTGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OHu93130 | |
Clone ID Related Accession (Same CDS sequence) | XM_017028090.1 | |
Accession Version | XM_017028090.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2703bp) Protein sequence SNP |
|
Vector | pcDNA3.1+/C-(K)DYK or customized vector | ![]() |
Clone information | Clone Map | ![]() |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-12-05 | |
Organism | Homo sapiens(human) | |
Product | E3 ubiquitin-protein ligase Itchy homolog isoform X2 | |
Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## |
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | XP_016883579.1 |
CDS | 176..2878 |
Translation |

Target ORF information:
Target ORF information:
|
![]() |

ORF Insert Sequence:
1 | ATGTCTGACA GTGGATCACA ACTTGGTTCA ATGGGTAGCC TCACCATGAA ATCACAGCTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.