ULK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ULK1
Entrez Gene ID	8408
Full Name	unc-51 like autophagy activating kinase 1
Synonyms	ATG1,ATG1A,UNC51,Unc51.1,hATG1
General protein information	Preferred Names unc-51 like autophagy activating kinase 1 Names serine/threonine-protein kinase ULK1 ATG1 autophagy related 1 homolog autophagy-related protein 1 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.33
Disorder MIM:	603168

mRNA and Protein(s)

mRNA	Protein	Name
XM_011538798.2	XP_011537100.1	serine/threonine-protein kinase ULK1 isoform X1
NM_003565.3	NP_003556.1	serine/threonine-protein kinase ULK1
XM_011538798.3	XP_011537100.1	serine/threonine-protein kinase ULK1 isoform X1
NM_003565.2	NP_003556.1	serine/threonine-protein kinase ULK1
XM_011538799.2	XP_011537101.1	serine/threonine-protein kinase ULK1 isoform X2
NM_003565.4	NP_003556.2	serine/threonine-protein kinase ULK1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ULK1	XP_415091.2
Xenopus tropicalis (tropical clawed frog)	ulk1	NP_001106388.1
Homo sapiens (human)	ULK1	NP_003556.1
Pan troglodytes (chimpanzee)	ULK1	XP_001148902.3
	LOC709746	XP_001105326.2
Bos taurus (cattle)	ULK1	NP_001192856.1
Danio rerio (zebrafish)	ulk1b	XP_002665971.2
Rattus norvegicus (Norway rat)	Ulk1	NP_001101811.1
Canis lupus familiaris (dog)	ULK1	XP_534635.3
Mus musculus (house mouse)	Ulk1	NP_033495.2

Gene Ontology

The following ULK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ULK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13668	NM_003565.3 Latest version!	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu61743	XM_011538798.3 Latest version!	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu109469	NM_003565.4 Latest version!	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu61744	XM_011538799.2 Latest version!	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OHu61743	XM_011538798.2	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu13668	NM_003565.2	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ULK1 ( NM_003565.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003556.1 Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), mRNA.

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CloneID	OHu13668
Clone ID Related Accession (Same CDS sequence)	NM_003565.3 , NM_003565.2
Accession Version	NM_003565.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase ULK1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131009.7, AF045458.1, CF131673.1, AB018265.2, BI822269.1 and BU729635.1. On Nov 22, 2018 this sequence version replaced NM_003565.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF045458.1, AB018265.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCATCCGCA GGTCAGGCAG CACCAGCCCC CTGGGCTTTG CAAGGGCCAG CCCCTCGCCC 
CCTGCCCACG CTGAGCATGG AGGCGTCCTG GCCAGGAAGA TGTCTCTGGG TGGAGGCCGG 
CCCTACACGC CATCTCCTCA AGTTGGAACC ATCCCTGAGC GGCCAGGCTG GAGCGGGACG 
CCCTCCCCAC AGGGAGCTGA GATGCGGGGT GGCAGGTCCC CTCGTCCAGG CTCCTCTGCA 
CCCGAGCACT CTCCCCGCAC TTCCGGGCTG GGCTGCCGCC TGCACAGCGC CCCCAACCTG 
TCTGACTTGC ACGTCGTCCG CCCCAAGCTG CCCAAACCCC CCACGGACCC CCTGGGAGCT 
GTGTTCAGCC CACCACAGGC CAGCCCTCCC CAGCCGTCCC ACGGCCTGCA GTCCTGCCGG 
AACCTGCGGG GCTCACCCAA GCTGCCCGAC TTCCTGCAGC GAAACCCCCT GCCCCCCATC 
CTGGGCTCCC CCACCAAGGC TGTGCCCTCC TTTGACTTCC CGAAGACCCC CAGCTCCCAG 
AACCTGCTGG CCCTCCTAGC CCGGCAGGGC GTGGTGATGA CGCCCCCTCG AAACCGGACG 
CTGCCCGACC TCTCGGAGGT GGGACCCTTC CATGGTCAGC CGTTGGGCCC TGGCCTGCGG 
CCAGGCGAGG ACCCCAAGGG CCCCTTTGGC CGGTCTTTCA GCACCAGCCG CCTCACTGAC 
CTGCTCCTTA AGGCGGCGTT TGGGACACAA GCCCCGGACC CGGGCAGCAC GGAGAGCCTG 
CAGGAGAAGC CCATGGAGAT CGCACCCTCA GCTGGCTTTG GAGGGAGCCT GCACCCAGGA 
GCCCGTGCTG GGGGCACCAG CAGCCCTTCC CCGGTGGTCT TCACCGTGGG CTCTCCCCCG 
AGCGGGAGCA CGCCCCCCCA GGGCCCCCGC ACCAGGATGT TCTCAGCGGG CCCCACTGGC 
TCTGCCAGCT CTTCTGCCCG CCACCTGGTG CCTGGGCCCT GCAGCGAGGC CCCAGCCCCT 
GAGCTCCCTG CTCCAGGACA CGGCTGCAGC TTTGCCGACC CCATTGCTGC GAACCTGGAG 
GGGGCTGTGA CCTTCGAGGC CCCCGACCTC CCTGAGGAGA CCCTCATGGA GCAAGAGCAC 
ACGGAGATCC TGCGTGGCCT GCGCTTCACG CTGCTGTTCG TGCAGCACGT CCTGGAGATC 
GCAGCCCTGA AGGGCAGCGC CAGTGAGGCG GCGGGGGGCC CTGAGTACCA GCTGCAGGAG 
AGTGTGGTGG CCGACCAGAT CAGCCTGCTG AGCCGAGAAT GGGGCTTCGC GGAACAGCTG 
GTGCTGTACC TGAAGGTGGC CGAGCTACTG TCCTCCGGCC TGCAAAGTGC CATCGACCAG 
ATCCGGGCCG GCAAGCTCTG CCTGTCGTCC ACTGTGAAGC AGGTGGTGCG CAGGCTGAAT 
GAGCTGTACA AGGCCAGCGT GGTGTCCTGC CAGGGCCTGA GCCTGCGGCT GCAGCGCTTC 
TTCCTGGACA AGCAGCGGCT CCTGGACCGC ATTCACAGCA TCACTGCCGA GAGGCTCATC 
TTCAGCCACG CTGTGCAGAT GGTGCAGTCG GCTGCCCTGG ACGAGATGTT CCAGCACCGT 
GAGGGCTGCG TCCCACGCTA CCACAAGGCC CTGCTGCTCC TGGAGGGGCT GCAGCACATG 
CTCTCGGACC AGGCCGACAT CGAGAACGTC ACCAAGTGCA AGCTGTGCAT TGAGCGGAGA 
CTCTCGGCGC TGCTGACTGG CATCTGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003556.1
CDS	381..3533
Translation	MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPIAANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA

Target ORF information:

RefSeq Version	NM_003565.3
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003565.3

ORF Insert Sequence:

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCATCCGCA GGTCAGGCAG CACCAGCCCC CTGGGCTTTG CAAGGGCCAG CCCCTCGCCC 
CCTGCCCACG CTGAGCATGG AGGCGTCCTG GCCAGGAAGA TGTCTCTGGG TGGAGGCCGG 
CCCTACACGC CATCTCCTCA AGTTGGAACC ATCCCTGAGC GGCCAGGCTG GAGCGGGACG 
CCCTCCCCAC AGGGAGCTGA GATGCGGGGT GGCAGGTCCC CTCGTCCAGG CTCCTCTGCA 
CCCGAGCACT CTCCCCGCAC TTCCGGGCTG GGCTGCCGCC TGCACAGCGC CCCCAACCTG 
TCTGACTTGC ACGTCGTCCG CCCCAAGCTG CCCAAACCCC CCACGGACCC CCTGGGAGCT 
GTGTTCAGCC CACCACAGGC CAGCCCTCCC CAGCCGTCCC ACGGCCTGCA GTCCTGCCGG 
AACCTGCGGG GCTCACCCAA GCTGCCCGAC TTCCTGCAGC GAAACCCCCT GCCCCCCATC 
CTGGGCTCCC CCACCAAGGC TGTGCCCTCC TTTGACTTCC CGAAGACCCC CAGCTCCCAG 
AACCTGCTGG CCCTCCTAGC CCGGCAGGGC GTGGTGATGA CGCCCCCTCG AAACCGGACG 
CTGCCCGACC TCTCGGAGGT GGGACCCTTC CATGGTCAGC CGTTGGGCCC TGGCCTGCGG 
CCAGGCGAGG ACCCCAAGGG CCCCTTTGGC CGGTCTTTCA GCACCAGCCG CCTCACTGAC 
CTGCTCCTTA AGGCGGCGTT TGGGACACAA GCCCCGGACC CGGGCAGCAC GGAGAGCCTG 
CAGGAGAAGC CCATGGAGAT CGCACCCTCA GCTGGCTTTG GAGGGAGCCT GCACCCAGGA 
GCCCGTGCTG GGGGCACCAG CAGCCCTTCC CCGGTGGTCT TCACCGTGGG CTCTCCCCCG 
AGCGGGAGCA CGCCCCCCCA GGGCCCCCGC ACCAGGATGT TCTCAGCGGG CCCCACTGGC 
TCTGCCAGCT CTTCTGCCCG CCACCTGGTG CCTGGGCCCT GCAGCGAGGC CCCAGCCCCT 
GAGCTCCCTG CTCCAGGACA CGGCTGCAGC TTTGCCGACC CCATTGCTGC GAACCTGGAG 
GGGGCTGTGA CCTTCGAGGC CCCCGACCTC CCTGAGGAGA CCCTCATGGA GCAAGAGCAC 
ACGGAGATCC TGCGTGGCCT GCGCTTCACG CTGCTGTTCG TGCAGCACGT CCTGGAGATC 
GCAGCCCTGA AGGGCAGCGC CAGTGAGGCG GCGGGGGGCC CTGAGTACCA GCTGCAGGAG 
AGTGTGGTGG CCGACCAGAT CAGCCTGCTG AGCCGAGAAT GGGGCTTCGC GGAACAGCTG 
GTGCTGTACC TGAAGGTGGC CGAGCTACTG TCCTCCGGCC TGCAAAGTGC CATCGACCAG 
ATCCGGGCCG GCAAGCTCTG CCTGTCGTCC ACTGTGAAGC AGGTGGTGCG CAGGCTGAAT 
GAGCTGTACA AGGCCAGCGT GGTGTCCTGC CAGGGCCTGA GCCTGCGGCT GCAGCGCTTC 
TTCCTGGACA AGCAGCGGCT CCTGGACCGC ATTCACAGCA TCACTGCCGA GAGGCTCATC 
TTCAGCCACG CTGTGCAGAT GGTGCAGTCG GCTGCCCTGG ACGAGATGTT CCAGCACCGT 
GAGGGCTGCG TCCCACGCTA CCACAAGGCC CTGCTGCTCC TGGAGGGGCT GCAGCACATG 
CTCTCGGACC AGGCCGACAT CGAGAACGTC ACCAAGTGCA AGCTGTGCAT TGAGCGGAGA 
CTCTCGGCGC TGCTGACTGG CATCTGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ULK1 ( NM_003565.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003556.1 Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), mRNA.

Price & Availability↑

CloneID	OHu13668
Clone ID Related Accession (Same CDS sequence)	NM_003565.3 , NM_003565.2
Accession Version	NM_003565.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase ULK1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF045458.1, CF131673.1, AB018265.2, BI822269.1 and BU729635.1. On Mar 25, 2009 this sequence version replaced NM_003565.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF045458.1, AB018265.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCATCCGCA GGTCAGGCAG CACCAGCCCC CTGGGCTTTG CAAGGGCCAG CCCCTCGCCC 
CCTGCCCACG CTGAGCATGG AGGCGTCCTG GCCAGGAAGA TGTCTCTGGG TGGAGGCCGG 
CCCTACACGC CATCTCCTCA AGTTGGAACC ATCCCTGAGC GGCCAGGCTG GAGCGGGACG 
CCCTCCCCAC AGGGAGCTGA GATGCGGGGT GGCAGGTCCC CTCGTCCAGG CTCCTCTGCA 
CCCGAGCACT CTCCCCGCAC TTCCGGGCTG GGCTGCCGCC TGCACAGCGC CCCCAACCTG 
TCTGACTTGC ACGTCGTCCG CCCCAAGCTG CCCAAACCCC CCACGGACCC CCTGGGAGCT 
GTGTTCAGCC CACCACAGGC CAGCCCTCCC CAGCCGTCCC ACGGCCTGCA GTCCTGCCGG 
AACCTGCGGG GCTCACCCAA GCTGCCCGAC TTCCTGCAGC GAAACCCCCT GCCCCCCATC 
CTGGGCTCCC CCACCAAGGC TGTGCCCTCC TTTGACTTCC CGAAGACCCC CAGCTCCCAG 
AACCTGCTGG CCCTCCTAGC CCGGCAGGGC GTGGTGATGA CGCCCCCTCG AAACCGGACG 
CTGCCCGACC TCTCGGAGGT GGGACCCTTC CATGGTCAGC CGTTGGGCCC TGGCCTGCGG 
CCAGGCGAGG ACCCCAAGGG CCCCTTTGGC CGGTCTTTCA GCACCAGCCG CCTCACTGAC 
CTGCTCCTTA AGGCGGCGTT TGGGACACAA GCCCCGGACC CGGGCAGCAC GGAGAGCCTG 
CAGGAGAAGC CCATGGAGAT CGCACCCTCA GCTGGCTTTG GAGGGAGCCT GCACCCAGGA 
GCCCGTGCTG GGGGCACCAG CAGCCCTTCC CCGGTGGTCT TCACCGTGGG CTCTCCCCCG 
AGCGGGAGCA CGCCCCCCCA GGGCCCCCGC ACCAGGATGT TCTCAGCGGG CCCCACTGGC 
TCTGCCAGCT CTTCTGCCCG CCACCTGGTG CCTGGGCCCT GCAGCGAGGC CCCAGCCCCT 
GAGCTCCCTG CTCCAGGACA CGGCTGCAGC TTTGCCGACC CCATTGCTGC GAACCTGGAG 
GGGGCTGTGA CCTTCGAGGC CCCCGACCTC CCTGAGGAGA CCCTCATGGA GCAAGAGCAC 
ACGGAGATCC TGCGTGGCCT GCGCTTCACG CTGCTGTTCG TGCAGCACGT CCTGGAGATC 
GCAGCCCTGA AGGGCAGCGC CAGTGAGGCG GCGGGGGGCC CTGAGTACCA GCTGCAGGAG 
AGTGTGGTGG CCGACCAGAT CAGCCTGCTG AGCCGAGAAT GGGGCTTCGC GGAACAGCTG 
GTGCTGTACC TGAAGGTGGC CGAGCTACTG TCCTCCGGCC TGCAAAGTGC CATCGACCAG 
ATCCGGGCCG GCAAGCTCTG CCTGTCGTCC ACTGTGAAGC AGGTGGTGCG CAGGCTGAAT 
GAGCTGTACA AGGCCAGCGT GGTGTCCTGC CAGGGCCTGA GCCTGCGGCT GCAGCGCTTC 
TTCCTGGACA AGCAGCGGCT CCTGGACCGC ATTCACAGCA TCACTGCCGA GAGGCTCATC 
TTCAGCCACG CTGTGCAGAT GGTGCAGTCG GCTGCCCTGG ACGAGATGTT CCAGCACCGT 
GAGGGCTGCG TCCCACGCTA CCACAAGGCC CTGCTGCTCC TGGAGGGGCT GCAGCACATG 
CTCTCGGACC AGGCCGACAT CGAGAACGTC ACCAAGTGCA AGCTGTGCAT TGAGCGGAGA 
CTCTCGGCGC TGCTGACTGG CATCTGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003556.1
CDS	269..3421
Translation	MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPIAANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA

Target ORF information:

RefSeq Version	NM_003565.2
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003565.2

ORF Insert Sequence:

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCATCCGCA GGTCAGGCAG CACCAGCCCC CTGGGCTTTG CAAGGGCCAG CCCCTCGCCC 
CCTGCCCACG CTGAGCATGG AGGCGTCCTG GCCAGGAAGA TGTCTCTGGG TGGAGGCCGG 
CCCTACACGC CATCTCCTCA AGTTGGAACC ATCCCTGAGC GGCCAGGCTG GAGCGGGACG 
CCCTCCCCAC AGGGAGCTGA GATGCGGGGT GGCAGGTCCC CTCGTCCAGG CTCCTCTGCA 
CCCGAGCACT CTCCCCGCAC TTCCGGGCTG GGCTGCCGCC TGCACAGCGC CCCCAACCTG 
TCTGACTTGC ACGTCGTCCG CCCCAAGCTG CCCAAACCCC CCACGGACCC CCTGGGAGCT 
GTGTTCAGCC CACCACAGGC CAGCCCTCCC CAGCCGTCCC ACGGCCTGCA GTCCTGCCGG 
AACCTGCGGG GCTCACCCAA GCTGCCCGAC TTCCTGCAGC GAAACCCCCT GCCCCCCATC 
CTGGGCTCCC CCACCAAGGC TGTGCCCTCC TTTGACTTCC CGAAGACCCC CAGCTCCCAG 
AACCTGCTGG CCCTCCTAGC CCGGCAGGGC GTGGTGATGA CGCCCCCTCG AAACCGGACG 
CTGCCCGACC TCTCGGAGGT GGGACCCTTC CATGGTCAGC CGTTGGGCCC TGGCCTGCGG 
CCAGGCGAGG ACCCCAAGGG CCCCTTTGGC CGGTCTTTCA GCACCAGCCG CCTCACTGAC 
CTGCTCCTTA AGGCGGCGTT TGGGACACAA GCCCCGGACC CGGGCAGCAC GGAGAGCCTG 
CAGGAGAAGC CCATGGAGAT CGCACCCTCA GCTGGCTTTG GAGGGAGCCT GCACCCAGGA 
GCCCGTGCTG GGGGCACCAG CAGCCCTTCC CCGGTGGTCT TCACCGTGGG CTCTCCCCCG 
AGCGGGAGCA CGCCCCCCCA GGGCCCCCGC ACCAGGATGT TCTCAGCGGG CCCCACTGGC 
TCTGCCAGCT CTTCTGCCCG CCACCTGGTG CCTGGGCCCT GCAGCGAGGC CCCAGCCCCT 
GAGCTCCCTG CTCCAGGACA CGGCTGCAGC TTTGCCGACC CCATTGCTGC GAACCTGGAG 
GGGGCTGTGA CCTTCGAGGC CCCCGACCTC CCTGAGGAGA CCCTCATGGA GCAAGAGCAC 
ACGGAGATCC TGCGTGGCCT GCGCTTCACG CTGCTGTTCG TGCAGCACGT CCTGGAGATC 
GCAGCCCTGA AGGGCAGCGC CAGTGAGGCG GCGGGGGGCC CTGAGTACCA GCTGCAGGAG 
AGTGTGGTGG CCGACCAGAT CAGCCTGCTG AGCCGAGAAT GGGGCTTCGC GGAACAGCTG 
GTGCTGTACC TGAAGGTGGC CGAGCTACTG TCCTCCGGCC TGCAAAGTGC CATCGACCAG 
ATCCGGGCCG GCAAGCTCTG CCTGTCGTCC ACTGTGAAGC AGGTGGTGCG CAGGCTGAAT 
GAGCTGTACA AGGCCAGCGT GGTGTCCTGC CAGGGCCTGA GCCTGCGGCT GCAGCGCTTC 
TTCCTGGACA AGCAGCGGCT CCTGGACCGC ATTCACAGCA TCACTGCCGA GAGGCTCATC 
TTCAGCCACG CTGTGCAGAT GGTGCAGTCG GCTGCCCTGG ACGAGATGTT CCAGCACCGT 
GAGGGCTGCG TCCCACGCTA CCACAAGGCC CTGCTGCTCC TGGAGGGGCT GCAGCACATG 
CTCTCGGACC AGGCCGACAT CGAGAACGTC ACCAAGTGCA AGCTGTGCAT TGAGCGGAGA 
CTCTCGGCGC TGCTGACTGG CATCTGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ULK1 ( XM_011538798.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537100.1 Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61743
Clone ID Related Accession (Same CDS sequence)	XM_011538798.2 , XM_011538798.3
Accession Version	XM_011538798.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase ULK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538798.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCGTCCACA GGTCAGGCAG GGCTGCTGAG GCCTCCCCTC CTAATATCTG CCTTGTCTTC 
AGGTCCTCTG CCATCCGCAG GTCAGGCAGC ACCAGCCCCC TGGGCTTTGC AAGGGCCAGC 
CCCTCGCCCC CTGCCCACGC TGAGCATGGA GGCGTCCTGG CCAGGAAGAT GTCTCTGGGT 
GGAGGCCGGC CCTACACGCC ATCTCCTCAA GTTGGAACCA TCCCTGAGCG GCCAGGCTGG 
AGCGGGACGC CCTCCCCACA GGGAGCTGAG ATGCGGGGTG GCAGGTCCCC TCGTCCAGGC 
TCCTCTGCAC CCGAGCACTC TCCCCGCACT TCCGGGCTGG GCTGCCGCCT GCACAGCGCC 
CCCAACCTGT CTGACTTGCA CGTCGTCCGC CCCAAGCTGC CCAAACCCCC CACGGACCCC 
CTGGGAGCTG TGTTCAGCCC ACCACAGGCC AGCCCTCCCC AGCCGTCCCA CGGCCTGCAG 
TCCTGCCGGA ACCTGCGGGG CTCACCCAAG CTGCCCGACT TCCTGCAGCG AAACCCCCTG 
CCCCCCATCC TGGGCTCCCC CACCAAGGCT GTGCCCTCCT TTGACTTCCC GAAGACCCCC 
AGCTCCCAGA ACCTGCTGGC CCTCCTAGCC CGGCAGGGCG TGGTGATGAC GCCCCCTCGA 
AACCGGACGC TGCCCGACCT CTCGGAGGTG GGACCCTTCC ATGGTCAGCC GTTGGGCCCT 
GGCCTGCGGC CAGGCGAGGA CCCCAAGGGC CCCTTTGGCC GGTCTTTCAG CACCAGCCGC 
CTCACTGACC TGCTCCTTAA GGCGGCGTTT GGGACACAAG CCCCGGACCC GGGCAGCACG 
GAGAGCCTGC AGGAGAAGCC CATGGAGATC GCACCCTCAG CTGGCTTTGG AGGGAGCCTG 
CACCCAGGAG CCCGTGCTGG GGGCACCAGC AGCCCTTCCC CGGTGGTCTT CACCGTGGGC 
TCTCCCCCGA GCGGGAGCAC GCCCCCCCAG GGCCCCCGCA CCAGGATGTT CTCAGCGGGC 
CCCACTGGCT CTGCCAGCTC TTCTGCCCGC CACCTGGTGC CTGGGCCCTG CAGCGAGGCC 
CCAGCCCCTG AGCTCCCTGC TCCAGGACAC GGCTGCAGCT TTGCCGACCC CATTACTGCG 
AACCTGGAGG GGGCTGTGAC CTTCGAGGCC CCCGACCTCC CTGAGGAGAC CCTCATGGAG 
CAAGAGCACA CGGAGATCCT GCGTGGCCTG CGCTTCACGC TGCTGTTCGT GCAGCACGTC 
CTGGAGATCG CAGCCCTGAA GGGCAGCGCC AGTGAGGCGG CGGGGGGCCC TGAGTACCAG 
CTGCAGGAGA GTGTGGTGGC CGACCAGATC AGCCTGCTGA GCCGAGAATG GGGCTTCGCG 
GAACAGCTGG TGCTGTACCT GAAGGTGGCC GAGCTACTGT CCTCCGGCCT GCAAAGTGCC 
ATCGACCAGA TCCGGGCCGG CAAGCTCTGC CTGTCGTCCA CTGTGAAGCA GGTGGTGCGC 
AGGCTGAATG AGCTGTACAA GGCCAGCGTG GTGTCCTGCC AGGGCCTGAG CCTGCGGCTG 
CAGCGCTTCT TCCTGGACAA GCAGCGGCTC CTGGACCGCA TTCACAGCAT CACTGCCGAG 
AGGCTCATCT TCAGCCACGC TGTGCAGATG GTGCAGTCGG CTGCCCTGGA CGAGATGTTC 
CAGCACCGTG AGGGCTGCGT CCCACGCTAC CACAAGGCCC TGCTGCTCCT GGAGGGGCTG 
CAGCACATGC TCTCGGACCA GGCCGACATC GAGAACGTCA CCAAGTGCAA GCTGTGCATT 
GAGCGGAGAC TCTCGGCGCT GCTGACTGGC ATCTGTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537100.1
CDS	46..3267
Translation	MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAVHRSGRAAEASPPNICLVFRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA

Target ORF information:

RefSeq Version	XM_011538798.2
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538798.2

ORF Insert Sequence:

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCGTCCACA GGTCAGGCAG GGCTGCTGAG GCCTCCCCTC CTAATATCTG CCTTGTCTTC 
AGGTCCTCTG CCATCCGCAG GTCAGGCAGC ACCAGCCCCC TGGGCTTTGC AAGGGCCAGC 
CCCTCGCCCC CTGCCCACGC TGAGCATGGA GGCGTCCTGG CCAGGAAGAT GTCTCTGGGT 
GGAGGCCGGC CCTACACGCC ATCTCCTCAA GTTGGAACCA TCCCTGAGCG GCCAGGCTGG 
AGCGGGACGC CCTCCCCACA GGGAGCTGAG ATGCGGGGTG GCAGGTCCCC TCGTCCAGGC 
TCCTCTGCAC CCGAGCACTC TCCCCGCACT TCCGGGCTGG GCTGCCGCCT GCACAGCGCC 
CCCAACCTGT CTGACTTGCA CGTCGTCCGC CCCAAGCTGC CCAAACCCCC CACGGACCCC 
CTGGGAGCTG TGTTCAGCCC ACCACAGGCC AGCCCTCCCC AGCCGTCCCA CGGCCTGCAG 
TCCTGCCGGA ACCTGCGGGG CTCACCCAAG CTGCCCGACT TCCTGCAGCG AAACCCCCTG 
CCCCCCATCC TGGGCTCCCC CACCAAGGCT GTGCCCTCCT TTGACTTCCC GAAGACCCCC 
AGCTCCCAGA ACCTGCTGGC CCTCCTAGCC CGGCAGGGCG TGGTGATGAC GCCCCCTCGA 
AACCGGACGC TGCCCGACCT CTCGGAGGTG GGACCCTTCC ATGGTCAGCC GTTGGGCCCT 
GGCCTGCGGC CAGGCGAGGA CCCCAAGGGC CCCTTTGGCC GGTCTTTCAG CACCAGCCGC 
CTCACTGACC TGCTCCTTAA GGCGGCGTTT GGGACACAAG CCCCGGACCC GGGCAGCACG 
GAGAGCCTGC AGGAGAAGCC CATGGAGATC GCACCCTCAG CTGGCTTTGG AGGGAGCCTG 
CACCCAGGAG CCCGTGCTGG GGGCACCAGC AGCCCTTCCC CGGTGGTCTT CACCGTGGGC 
TCTCCCCCGA GCGGGAGCAC GCCCCCCCAG GGCCCCCGCA CCAGGATGTT CTCAGCGGGC 
CCCACTGGCT CTGCCAGCTC TTCTGCCCGC CACCTGGTGC CTGGGCCCTG CAGCGAGGCC 
CCAGCCCCTG AGCTCCCTGC TCCAGGACAC GGCTGCAGCT TTGCCGACCC CATTACTGCG 
AACCTGGAGG GGGCTGTGAC CTTCGAGGCC CCCGACCTCC CTGAGGAGAC CCTCATGGAG 
CAAGAGCACA CGGAGATCCT GCGTGGCCTG CGCTTCACGC TGCTGTTCGT GCAGCACGTC 
CTGGAGATCG CAGCCCTGAA GGGCAGCGCC AGTGAGGCGG CGGGGGGCCC TGAGTACCAG 
CTGCAGGAGA GTGTGGTGGC CGACCAGATC AGCCTGCTGA GCCGAGAATG GGGCTTCGCG 
GAACAGCTGG TGCTGTACCT GAAGGTGGCC GAGCTACTGT CCTCCGGCCT GCAAAGTGCC 
ATCGACCAGA TCCGGGCCGG CAAGCTCTGC CTGTCGTCCA CTGTGAAGCA GGTGGTGCGC 
AGGCTGAATG AGCTGTACAA GGCCAGCGTG GTGTCCTGCC AGGGCCTGAG CCTGCGGCTG 
CAGCGCTTCT TCCTGGACAA GCAGCGGCTC CTGGACCGCA TTCACAGCAT CACTGCCGAG 
AGGCTCATCT TCAGCCACGC TGTGCAGATG GTGCAGTCGG CTGCCCTGGA CGAGATGTTC 
CAGCACCGTG AGGGCTGCGT CCCACGCTAC CACAAGGCCC TGCTGCTCCT GGAGGGGCTG 
CAGCACATGC TCTCGGACCA GGCCGACATC GAGAACGTCA CCAAGTGCAA GCTGTGCATT 
GAGCGGAGAC TCTCGGCGCT GCTGACTGGC ATCTGTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ULK1 ( XM_011538798.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537100.1 Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61743
Clone ID Related Accession (Same CDS sequence)	XM_011538798.2 , XM_011538798.3
Accession Version	XM_011538798.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase ULK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011538798.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCGTCCACA GGTCAGGCAG GGCTGCTGAG GCCTCCCCTC CTAATATCTG CCTTGTCTTC 
AGGTCCTCTG CCATCCGCAG GTCAGGCAGC ACCAGCCCCC TGGGCTTTGC AAGGGCCAGC 
CCCTCGCCCC CTGCCCACGC TGAGCATGGA GGCGTCCTGG CCAGGAAGAT GTCTCTGGGT 
GGAGGCCGGC CCTACACGCC ATCTCCTCAA GTTGGAACCA TCCCTGAGCG GCCAGGCTGG 
AGCGGGACGC CCTCCCCACA GGGAGCTGAG ATGCGGGGTG GCAGGTCCCC TCGTCCAGGC 
TCCTCTGCAC CCGAGCACTC TCCCCGCACT TCCGGGCTGG GCTGCCGCCT GCACAGCGCC 
CCCAACCTGT CTGACTTGCA CGTCGTCCGC CCCAAGCTGC CCAAACCCCC CACGGACCCC 
CTGGGAGCTG TGTTCAGCCC ACCACAGGCC AGCCCTCCCC AGCCGTCCCA CGGCCTGCAG 
TCCTGCCGGA ACCTGCGGGG CTCACCCAAG CTGCCCGACT TCCTGCAGCG AAACCCCCTG 
CCCCCCATCC TGGGCTCCCC CACCAAGGCT GTGCCCTCCT TTGACTTCCC GAAGACCCCC 
AGCTCCCAGA ACCTGCTGGC CCTCCTAGCC CGGCAGGGCG TGGTGATGAC GCCCCCTCGA 
AACCGGACGC TGCCCGACCT CTCGGAGGTG GGACCCTTCC ATGGTCAGCC GTTGGGCCCT 
GGCCTGCGGC CAGGCGAGGA CCCCAAGGGC CCCTTTGGCC GGTCTTTCAG CACCAGCCGC 
CTCACTGACC TGCTCCTTAA GGCGGCGTTT GGGACACAAG CCCCGGACCC GGGCAGCACG 
GAGAGCCTGC AGGAGAAGCC CATGGAGATC GCACCCTCAG CTGGCTTTGG AGGGAGCCTG 
CACCCAGGAG CCCGTGCTGG GGGCACCAGC AGCCCTTCCC CGGTGGTCTT CACCGTGGGC 
TCTCCCCCGA GCGGGAGCAC GCCCCCCCAG GGCCCCCGCA CCAGGATGTT CTCAGCGGGC 
CCCACTGGCT CTGCCAGCTC TTCTGCCCGC CACCTGGTGC CTGGGCCCTG CAGCGAGGCC 
CCAGCCCCTG AGCTCCCTGC TCCAGGACAC GGCTGCAGCT TTGCCGACCC CATTACTGCG 
AACCTGGAGG GGGCTGTGAC CTTCGAGGCC CCCGACCTCC CTGAGGAGAC CCTCATGGAG 
CAAGAGCACA CGGAGATCCT GCGTGGCCTG CGCTTCACGC TGCTGTTCGT GCAGCACGTC 
CTGGAGATCG CAGCCCTGAA GGGCAGCGCC AGTGAGGCGG CGGGGGGCCC TGAGTACCAG 
CTGCAGGAGA GTGTGGTGGC CGACCAGATC AGCCTGCTGA GCCGAGAATG GGGCTTCGCG 
GAACAGCTGG TGCTGTACCT GAAGGTGGCC GAGCTACTGT CCTCCGGCCT GCAAAGTGCC 
ATCGACCAGA TCCGGGCCGG CAAGCTCTGC CTGTCGTCCA CTGTGAAGCA GGTGGTGCGC 
AGGCTGAATG AGCTGTACAA GGCCAGCGTG GTGTCCTGCC AGGGCCTGAG CCTGCGGCTG 
CAGCGCTTCT TCCTGGACAA GCAGCGGCTC CTGGACCGCA TTCACAGCAT CACTGCCGAG 
AGGCTCATCT TCAGCCACGC TGTGCAGATG GTGCAGTCGG CTGCCCTGGA CGAGATGTTC 
CAGCACCGTG AGGGCTGCGT CCCACGCTAC CACAAGGCCC TGCTGCTCCT GGAGGGGCTG 
CAGCACATGC TCTCGGACCA GGCCGACATC GAGAACGTCA CCAAGTGCAA GCTGTGCATT 
GAGCGGAGAC TCTCGGCGCT GCTGACTGGC ATCTGTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537100.1
CDS	47..3268
Translation	MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAVHRSGRAAEASPPNICLVFRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA

Target ORF information:

RefSeq Version	XM_011538798.3
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538798.3

ORF Insert Sequence:

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCGTCCACA GGTCAGGCAG GGCTGCTGAG GCCTCCCCTC CTAATATCTG CCTTGTCTTC 
AGGTCCTCTG CCATCCGCAG GTCAGGCAGC ACCAGCCCCC TGGGCTTTGC AAGGGCCAGC 
CCCTCGCCCC CTGCCCACGC TGAGCATGGA GGCGTCCTGG CCAGGAAGAT GTCTCTGGGT 
GGAGGCCGGC CCTACACGCC ATCTCCTCAA GTTGGAACCA TCCCTGAGCG GCCAGGCTGG 
AGCGGGACGC CCTCCCCACA GGGAGCTGAG ATGCGGGGTG GCAGGTCCCC TCGTCCAGGC 
TCCTCTGCAC CCGAGCACTC TCCCCGCACT TCCGGGCTGG GCTGCCGCCT GCACAGCGCC 
CCCAACCTGT CTGACTTGCA CGTCGTCCGC CCCAAGCTGC CCAAACCCCC CACGGACCCC 
CTGGGAGCTG TGTTCAGCCC ACCACAGGCC AGCCCTCCCC AGCCGTCCCA CGGCCTGCAG 
TCCTGCCGGA ACCTGCGGGG CTCACCCAAG CTGCCCGACT TCCTGCAGCG AAACCCCCTG 
CCCCCCATCC TGGGCTCCCC CACCAAGGCT GTGCCCTCCT TTGACTTCCC GAAGACCCCC 
AGCTCCCAGA ACCTGCTGGC CCTCCTAGCC CGGCAGGGCG TGGTGATGAC GCCCCCTCGA 
AACCGGACGC TGCCCGACCT CTCGGAGGTG GGACCCTTCC ATGGTCAGCC GTTGGGCCCT 
GGCCTGCGGC CAGGCGAGGA CCCCAAGGGC CCCTTTGGCC GGTCTTTCAG CACCAGCCGC 
CTCACTGACC TGCTCCTTAA GGCGGCGTTT GGGACACAAG CCCCGGACCC GGGCAGCACG 
GAGAGCCTGC AGGAGAAGCC CATGGAGATC GCACCCTCAG CTGGCTTTGG AGGGAGCCTG 
CACCCAGGAG CCCGTGCTGG GGGCACCAGC AGCCCTTCCC CGGTGGTCTT CACCGTGGGC 
TCTCCCCCGA GCGGGAGCAC GCCCCCCCAG GGCCCCCGCA CCAGGATGTT CTCAGCGGGC 
CCCACTGGCT CTGCCAGCTC TTCTGCCCGC CACCTGGTGC CTGGGCCCTG CAGCGAGGCC 
CCAGCCCCTG AGCTCCCTGC TCCAGGACAC GGCTGCAGCT TTGCCGACCC CATTACTGCG 
AACCTGGAGG GGGCTGTGAC CTTCGAGGCC CCCGACCTCC CTGAGGAGAC CCTCATGGAG 
CAAGAGCACA CGGAGATCCT GCGTGGCCTG CGCTTCACGC TGCTGTTCGT GCAGCACGTC 
CTGGAGATCG CAGCCCTGAA GGGCAGCGCC AGTGAGGCGG CGGGGGGCCC TGAGTACCAG 
CTGCAGGAGA GTGTGGTGGC CGACCAGATC AGCCTGCTGA GCCGAGAATG GGGCTTCGCG 
GAACAGCTGG TGCTGTACCT GAAGGTGGCC GAGCTACTGT CCTCCGGCCT GCAAAGTGCC 
ATCGACCAGA TCCGGGCCGG CAAGCTCTGC CTGTCGTCCA CTGTGAAGCA GGTGGTGCGC 
AGGCTGAATG AGCTGTACAA GGCCAGCGTG GTGTCCTGCC AGGGCCTGAG CCTGCGGCTG 
CAGCGCTTCT TCCTGGACAA GCAGCGGCTC CTGGACCGCA TTCACAGCAT CACTGCCGAG 
AGGCTCATCT TCAGCCACGC TGTGCAGATG GTGCAGTCGG CTGCCCTGGA CGAGATGTTC 
CAGCACCGTG AGGGCTGCGT CCCACGCTAC CACAAGGCCC TGCTGCTCCT GGAGGGGCTG 
CAGCACATGC TCTCGGACCA GGCCGACATC GAGAACGTCA CCAAGTGCAA GCTGTGCATT 
GAGCGGAGAC TCTCGGCGCT GCTGACTGGC ATCTGTGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ULK1 ( NM_003565.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003556.2 Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), mRNA.

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CloneID	OHu109469
Clone ID Related Accession (Same CDS sequence)	NM_003565.4
Accession Version	NM_003565.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase ULK1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131009.7. On Sep 5, 2019 this sequence version replaced NM_003565.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF045458.1, AB018265.2 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000321867.6/ ENSP00000324560.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCATCCGCA GGTCAGGCAG CACCAGCCCC CTGGGCTTTG CAAGGGCCAG CCCCTCGCCC 
CCTGCCCACG CTGAGCATGG AGGCGTCCTG GCCAGGAAGA TGTCTCTGGG TGGAGGCCGG 
CCCTACACGC CATCTCCTCA AGTTGGAACC ATCCCTGAGC GGCCAGGCTG GAGCGGGACG 
CCCTCCCCAC AGGGAGCTGA GATGCGGGGT GGCAGGTCCC CTCGTCCAGG CTCCTCTGCA 
CCCGAGCACT CTCCCCGCAC TTCCGGGCTG GGCTGCCGCC TGCACAGCGC CCCCAACCTG 
TCTGACTTGC ACGTCGTCCG CCCCAAGCTG CCCAAACCCC CCACGGACCC CCTGGGAGCT 
GTGTTCAGCC CACCACAGGC CAGCCCTCCC CAGCCGTCCC ACGGCCTGCA GTCCTGCCGG 
AACCTGCGGG GCTCACCCAA GCTGCCCGAC TTCCTGCAGC GAAACCCCCT GCCCCCCATC 
CTGGGCTCCC CCACCAAGGC TGTGCCCTCC TTTGACTTCC CGAAGACCCC CAGCTCCCAG 
AACCTGCTGG CCCTCCTAGC CCGGCAGGGC GTGGTGATGA CGCCCCCTCG AAACCGGACG 
CTGCCCGACC TCTCGGAGGT GGGACCCTTC CATGGTCAGC CGTTGGGCCC TGGCCTGCGG 
CCAGGCGAGG ACCCCAAGGG CCCCTTTGGC CGGTCTTTCA GCACCAGCCG CCTCACTGAC 
CTGCTCCTTA AGGCGGCGTT TGGGACACAA GCCCCGGACC CGGGCAGCAC GGAGAGCCTG 
CAGGAGAAGC CCATGGAGAT CGCACCCTCA GCTGGCTTTG GAGGGAGCCT GCACCCAGGA 
GCCCGTGCTG GGGGCACCAG CAGCCCTTCC CCGGTGGTCT TCACCGTGGG CTCTCCCCCG 
AGCGGGAGCA CGCCCCCCCA GGGCCCCCGC ACCAGGATGT TCTCAGCGGG CCCCACTGGC 
TCTGCCAGCT CTTCTGCCCG CCACCTGGTG CCTGGGCCCT GCAGCGAGGC CCCAGCCCCT 
GAGCTCCCTG CTCCAGGACA CGGCTGCAGC TTTGCCGACC CCATTACTGC GAACCTGGAG 
GGGGCTGTGA CCTTCGAGGC CCCCGACCTC CCTGAGGAGA CCCTCATGGA GCAAGAGCAC 
ACGGAGATCC TGCGTGGCCT GCGCTTCACG CTGCTGTTCG TGCAGCACGT CCTGGAGATC 
GCAGCCCTGA AGGGCAGCGC CAGTGAGGCG GCGGGGGGCC CTGAGTACCA GCTGCAGGAG 
AGTGTGGTGG CCGACCAGAT CAGCCTGCTG AGCCGAGAAT GGGGCTTCGC GGAACAGCTG 
GTGCTGTACC TGAAGGTGGC CGAGCTACTG TCCTCCGGCC TGCAAAGTGC CATCGACCAG 
ATCCGGGCCG GCAAGCTCTG CCTGTCGTCC ACTGTGAAGC AGGTGGTGCG CAGGCTGAAT 
GAGCTGTACA AGGCCAGCGT GGTGTCCTGC CAGGGCCTGA GCCTGCGGCT GCAGCGCTTC 
TTCCTGGACA AGCAGCGGCT CCTGGACCGC ATTCACAGCA TCACTGCCGA GAGGCTCATC 
TTCAGCCACG CTGTGCAGAT GGTGCAGTCG GCTGCCCTGG ACGAGATGTT CCAGCACCGT 
GAGGGCTGCG TCCCACGCTA CCACAAGGCC CTGCTGCTCC TGGAGGGGCT GCAGCACATG 
CTCTCGGACC AGGCCGACAT CGAGAACGTC ACCAAGTGCA AGCTGTGCAT TGAGCGGAGA 
CTCTCGGCGC TGCTGACTGG CATCTGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003556.2
CDS	381..3533
Translation	MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA

Target ORF information:

RefSeq Version	NM_003565.4
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003565.4

ORF Insert Sequence:

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCCTCT 
GCCATCCGCA GGTCAGGCAG CACCAGCCCC CTGGGCTTTG CAAGGGCCAG CCCCTCGCCC 
CCTGCCCACG CTGAGCATGG AGGCGTCCTG GCCAGGAAGA TGTCTCTGGG TGGAGGCCGG 
CCCTACACGC CATCTCCTCA AGTTGGAACC ATCCCTGAGC GGCCAGGCTG GAGCGGGACG 
CCCTCCCCAC AGGGAGCTGA GATGCGGGGT GGCAGGTCCC CTCGTCCAGG CTCCTCTGCA 
CCCGAGCACT CTCCCCGCAC TTCCGGGCTG GGCTGCCGCC TGCACAGCGC CCCCAACCTG 
TCTGACTTGC ACGTCGTCCG CCCCAAGCTG CCCAAACCCC CCACGGACCC CCTGGGAGCT 
GTGTTCAGCC CACCACAGGC CAGCCCTCCC CAGCCGTCCC ACGGCCTGCA GTCCTGCCGG 
AACCTGCGGG GCTCACCCAA GCTGCCCGAC TTCCTGCAGC GAAACCCCCT GCCCCCCATC 
CTGGGCTCCC CCACCAAGGC TGTGCCCTCC TTTGACTTCC CGAAGACCCC CAGCTCCCAG 
AACCTGCTGG CCCTCCTAGC CCGGCAGGGC GTGGTGATGA CGCCCCCTCG AAACCGGACG 
CTGCCCGACC TCTCGGAGGT GGGACCCTTC CATGGTCAGC CGTTGGGCCC TGGCCTGCGG 
CCAGGCGAGG ACCCCAAGGG CCCCTTTGGC CGGTCTTTCA GCACCAGCCG CCTCACTGAC 
CTGCTCCTTA AGGCGGCGTT TGGGACACAA GCCCCGGACC CGGGCAGCAC GGAGAGCCTG 
CAGGAGAAGC CCATGGAGAT CGCACCCTCA GCTGGCTTTG GAGGGAGCCT GCACCCAGGA 
GCCCGTGCTG GGGGCACCAG CAGCCCTTCC CCGGTGGTCT TCACCGTGGG CTCTCCCCCG 
AGCGGGAGCA CGCCCCCCCA GGGCCCCCGC ACCAGGATGT TCTCAGCGGG CCCCACTGGC 
TCTGCCAGCT CTTCTGCCCG CCACCTGGTG CCTGGGCCCT GCAGCGAGGC CCCAGCCCCT 
GAGCTCCCTG CTCCAGGACA CGGCTGCAGC TTTGCCGACC CCATTACTGC GAACCTGGAG 
GGGGCTGTGA CCTTCGAGGC CCCCGACCTC CCTGAGGAGA CCCTCATGGA GCAAGAGCAC 
ACGGAGATCC TGCGTGGCCT GCGCTTCACG CTGCTGTTCG TGCAGCACGT CCTGGAGATC 
GCAGCCCTGA AGGGCAGCGC CAGTGAGGCG GCGGGGGGCC CTGAGTACCA GCTGCAGGAG 
AGTGTGGTGG CCGACCAGAT CAGCCTGCTG AGCCGAGAAT GGGGCTTCGC GGAACAGCTG 
GTGCTGTACC TGAAGGTGGC CGAGCTACTG TCCTCCGGCC TGCAAAGTGC CATCGACCAG 
ATCCGGGCCG GCAAGCTCTG CCTGTCGTCC ACTGTGAAGC AGGTGGTGCG CAGGCTGAAT 
GAGCTGTACA AGGCCAGCGT GGTGTCCTGC CAGGGCCTGA GCCTGCGGCT GCAGCGCTTC 
TTCCTGGACA AGCAGCGGCT CCTGGACCGC ATTCACAGCA TCACTGCCGA GAGGCTCATC 
TTCAGCCACG CTGTGCAGAT GGTGCAGTCG GCTGCCCTGG ACGAGATGTT CCAGCACCGT 
GAGGGCTGCG TCCCACGCTA CCACAAGGCC CTGCTGCTCC TGGAGGGGCT GCAGCACATG 
CTCTCGGACC AGGCCGACAT CGAGAACGTC ACCAAGTGCA AGCTGTGCAT TGAGCGGAGA 
CTCTCGGCGC TGCTGACTGG CATCTGTGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ULK1 ( XM_011538799.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011537101.1 Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), transcript variant X2, mRNA.

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CloneID	OHu61744
Clone ID Related Accession (Same CDS sequence)	XM_011538799.2
Accession Version	XM_011538799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase ULK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCAGGC 
AGCACCAGCC CCCTGGGCTT TGCAAGGGCC AGCCCCTCGC CCCCTGCCCA CGCTGAGCAT 
GGAGGCGTCC TGGCCAGGAA GATGTCTCTG GGTGGAGGCC GGCCCTACAC GCCATCTCCT 
CAAGTTGGAA CCATCCCTGA GCGGCCAGGC TGGAGCGGGA CGCCCTCCCC ACAGGGAGCT 
GAGATGCGGG GTGGCAGGTC CCCTCGTCCA GGCTCCTCTG CACCCGAGCA CTCTCCCCGC 
ACTTCCGGGC TGGGCTGCCG CCTGCACAGC GCCCCCAACC TGTCTGACTT GCACGTCGTC 
CGCCCCAAGC TGCCCAAACC CCCCACGGAC CCCCTGGGAG CTGTGTTCAG CCCACCACAG 
GCCAGCCCTC CCCAGCCGTC CCACGGCCTG CAGTCCTGCC GGAACCTGCG GGGCTCACCC 
AAGCTGCCCG ACTTCCTGCA GCGAAACCCC CTGCCCCCCA TCCTGGGCTC CCCCACCAAG 
GCTGTGCCCT CCTTTGACTT CCCGAAGACC CCCAGCTCCC AGAACCTGCT GGCCCTCCTA 
GCCCGGCAGG GCGTGGTGAT GACGCCCCCT CGAAACCGGA CGCTGCCCGA CCTCTCGGAG 
GTGGGACCCT TCCATGGTCA GCCGTTGGGC CCTGGCCTGC GGCCAGGCGA GGACCCCAAG 
GGCCCCTTTG GCCGGTCTTT CAGCACCAGC CGCCTCACTG ACCTGCTCCT TAAGGCGGCG 
TTTGGGACAC AAGCCCCGGA CCCGGGCAGC ACGGAGAGCC TGCAGGAGAA GCCCATGGAG 
ATCGCACCCT CAGCTGGCTT TGGAGGGAGC CTGCACCCAG GAGCCCGTGC TGGGGGCACC 
AGCAGCCCTT CCCCGGTGGT CTTCACCGTG GGCTCTCCCC CGAGCGGGAG CACGCCCCCC 
CAGGGCCCCC GCACCAGGAT GTTCTCAGCG GGCCCCACTG GCTCTGCCAG CTCTTCTGCC 
CGCCACCTGG TGCCTGGGCC CTGCAGCGAG GCCCCAGCCC CTGAGCTCCC TGCTCCAGGA 
CACGGCTGCA GCTTTGCCGA CCCCATTACT GCGAACCTGG AGGGGGCTGT GACCTTCGAG 
GCCCCCGACC TCCCTGAGGA GACCCTCATG GAGCAAGAGC ACACGGAGAT CCTGCGTGGC 
CTGCGCTTCA CGCTGCTGTT CGTGCAGCAC GTCCTGGAGA TCGCAGCCCT GAAGGGCAGC 
GCCAGTGAGG CGGCGGGGGG CCCTGAGTAC CAGCTGCAGG AGAGTGTGGT GGCCGACCAG 
ATCAGCCTGC TGAGCCGAGA ATGGGGCTTC GCGGAACAGC TGGTGCTGTA CCTGAAGGTG 
GCCGAGCTAC TGTCCTCCGG CCTGCAAAGT GCCATCGACC AGATCCGGGC CGGCAAGCTC 
TGCCTGTCGT CCACTGTGAA GCAGGTGGTG CGCAGGCTGA ATGAGCTGTA CAAGGCCAGC 
GTGGTGTCCT GCCAGGGCCT GAGCCTGCGG CTGCAGCGCT TCTTCCTGGA CAAGCAGCGG 
CTCCTGGACC GCATTCACAG CATCACTGCC GAGAGGCTCA TCTTCAGCCA CGCTGTGCAG 
ATGGTGCAGT CGGCTGCCCT GGACGAGATG TTCCAGCACC GTGAGGGCTG CGTCCCACGC 
TACCACAAGG CCCTGCTGCT CCTGGAGGGG CTGCAGCACA TGCTCTCGGA CCAGGCCGAC 
ATCGAGAACG TCACCAAGTG CAAGCTGTGC ATTGAGCGGA GACTCTCGGC GCTGCTGACT 
GGCATCTGTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011537101.1
CDS	46..3180
Translation	MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA

Target ORF information:

RefSeq Version	XM_011538799.2
Organism	Homo sapiens(human)
Definition	Homo sapiens unc-51 like autophagy activating kinase 1 (ULK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538799.2

ORF Insert Sequence:

ATGGAGCCCG GCCGCGGCGG CACAGAGACC GTGGGCAAGT TCGAGTTCTC CCGCAAGGAC 
CTGATCGGCC ACGGCGCCTT CGCGGTGGTC TTCAAGGGCC GCCACCGCGA GAAGCACGAT 
TTGGAGGTCG CCGTCAAGTG CATTAACAAG AAGAACCTCG CCAAGTCTCA GACGCTGCTG 
GGGAAGGAAA TCAAAATCCT GAAGGAACTG AAACATGAAA ACATCGTGGC CCTGTACGAC 
TTCCAGGAAA TGGCTAATTC TGTCTACCTG GTTATGGAGT ACTGCAACGG TGGGGACCTG 
GCCGACTACC TGCACGCCAT GCGCACGCTG AGCGAGGACA CCATCAGGCT CTTCCTGCAG 
CAGATCGCGG GCGCCATGCG GCTTCTGCAC AGCAAAGGCA TCATCCACCG CGACCTGAAA 
CCGCAGAACA TCCTGCTGTC CAACCCCGCC GGCCGCCGCG CCAACCCCAA CAGCATCCGC 
GTCAAGATCG CTGACTTCGG CTTCGCGCGG TACCTCCAGA GCAACATGAT GGCGGCCACA 
CTCTGCGGCT CCCCCATGTA CATGGCCCCC GAGGTCATCA TGTCCCAGCA CTACGACGGG 
AAGGCGGACC TGTGGAGCAT CGGCACCATC GTCTACCAGT GCCTGACGGG GAAGGCGCCC 
TTCCAGGCCA GCAGCCCCCA GGACCTGCGC CTGTTCTACG AGAAGAACAA GACGTTGGTC 
CCCACCATCC CCCGGGAGAC CTCGGCCCCG CTGCGGCAGC TGCTCCTGGC CCTACTGCAA 
CGCAACCACA AGGACCGCAT GGACTTCGAT GAGTTTTTTC ATCACCCTTT CCTCGATGCC 
AGCCCCTCGG TCAGGAAATC CCCACCCGTG CCTGTGCCCT CGTACCCAAG CTCGGGGTCC 
GGCAGCAGCT CCAGCAGCAG CTCCACCTCC CACCTGGCCT CCCCGCCGTC CCTGGGCGAG 
ATGCAGCAGC TGCAGAAGAC CCTGGCCTCC CCGGCTGACA CCGCTGGCTT CCTGCACAGC 
TCCCGGGACT CTGGTGGCAG CAAGGACTCT TCCTGTGACA CAGACGACTT CGTCATGGTC 
CCCGCGCAGT TTCCAGGTGA CCTGGTGGCT GAGGCGCCCA GTGCCAAACC CCCGCCAGAC 
AGCCTGATGT GCAGTGGGAG CTCACTGGTG GCCTCTGCGG GCTTGGAGAG CCACGGCCGG 
ACCCCATCTC CATCCCCACC CTGCAGCAGC TCCCCCAGTC CCTCAGGCCG GGCTGGCCCG 
TTCTCCAGCA GCAGGTGCGG CGCCTCTGTC CCCATCCCAG TCCCCACGCA GGTGCAGAAC 
TACCAGCGCA TTGAGCGAAA CCTGCAGTCA CCCACCCAGT TCCAAACACC TCGGTCAGGC 
AGCACCAGCC CCCTGGGCTT TGCAAGGGCC AGCCCCTCGC CCCCTGCCCA CGCTGAGCAT 
GGAGGCGTCC TGGCCAGGAA GATGTCTCTG GGTGGAGGCC GGCCCTACAC GCCATCTCCT 
CAAGTTGGAA CCATCCCTGA GCGGCCAGGC TGGAGCGGGA CGCCCTCCCC ACAGGGAGCT 
GAGATGCGGG GTGGCAGGTC CCCTCGTCCA GGCTCCTCTG CACCCGAGCA CTCTCCCCGC 
ACTTCCGGGC TGGGCTGCCG CCTGCACAGC GCCCCCAACC TGTCTGACTT GCACGTCGTC 
CGCCCCAAGC TGCCCAAACC CCCCACGGAC CCCCTGGGAG CTGTGTTCAG CCCACCACAG 
GCCAGCCCTC CCCAGCCGTC CCACGGCCTG CAGTCCTGCC GGAACCTGCG GGGCTCACCC 
AAGCTGCCCG ACTTCCTGCA GCGAAACCCC CTGCCCCCCA TCCTGGGCTC CCCCACCAAG 
GCTGTGCCCT CCTTTGACTT CCCGAAGACC CCCAGCTCCC AGAACCTGCT GGCCCTCCTA 
GCCCGGCAGG GCGTGGTGAT GACGCCCCCT CGAAACCGGA CGCTGCCCGA CCTCTCGGAG 
GTGGGACCCT TCCATGGTCA GCCGTTGGGC CCTGGCCTGC GGCCAGGCGA GGACCCCAAG 
GGCCCCTTTG GCCGGTCTTT CAGCACCAGC CGCCTCACTG ACCTGCTCCT TAAGGCGGCG 
TTTGGGACAC AAGCCCCGGA CCCGGGCAGC ACGGAGAGCC TGCAGGAGAA GCCCATGGAG 
ATCGCACCCT CAGCTGGCTT TGGAGGGAGC CTGCACCCAG GAGCCCGTGC TGGGGGCACC 
AGCAGCCCTT CCCCGGTGGT CTTCACCGTG GGCTCTCCCC CGAGCGGGAG CACGCCCCCC 
CAGGGCCCCC GCACCAGGAT GTTCTCAGCG GGCCCCACTG GCTCTGCCAG CTCTTCTGCC 
CGCCACCTGG TGCCTGGGCC CTGCAGCGAG GCCCCAGCCC CTGAGCTCCC TGCTCCAGGA 
CACGGCTGCA GCTTTGCCGA CCCCATTACT GCGAACCTGG AGGGGGCTGT GACCTTCGAG 
GCCCCCGACC TCCCTGAGGA GACCCTCATG GAGCAAGAGC ACACGGAGAT CCTGCGTGGC 
CTGCGCTTCA CGCTGCTGTT CGTGCAGCAC GTCCTGGAGA TCGCAGCCCT GAAGGGCAGC 
GCCAGTGAGG CGGCGGGGGG CCCTGAGTAC CAGCTGCAGG AGAGTGTGGT GGCCGACCAG 
ATCAGCCTGC TGAGCCGAGA ATGGGGCTTC GCGGAACAGC TGGTGCTGTA CCTGAAGGTG 
GCCGAGCTAC TGTCCTCCGG CCTGCAAAGT GCCATCGACC AGATCCGGGC CGGCAAGCTC 
TGCCTGTCGT CCACTGTGAA GCAGGTGGTG CGCAGGCTGA ATGAGCTGTA CAAGGCCAGC 
GTGGTGTCCT GCCAGGGCCT GAGCCTGCGG CTGCAGCGCT TCTTCCTGGA CAAGCAGCGG 
CTCCTGGACC GCATTCACAG CATCACTGCC GAGAGGCTCA TCTTCAGCCA CGCTGTGCAG 
ATGGTGCAGT CGGCTGCCCT GGACGAGATG TTCCAGCACC GTGAGGGCTG CGTCCCACGC 
TACCACAAGG CCCTGCTGCT CCTGGAGGGG CTGCAGCACA TGCTCTCGGA CCAGGCCGAC 
ATCGAGAACG TCACCAAGTG CAAGCTGTGC ATTGAGCGGA GACTCTCGGC GCTGCTGACT 
GGCATCTGTG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ULK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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