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RECQL4 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol RECQL4
Entrez Gene ID 9401
Full Name RecQ like helicase 4
Synonyms RECQ4
General protein information
Preferred Names

RecQ like helicase 4

Names

ATP-dependent DNA helicase Q4
DNA helicase, RecQ-like, type 4
RecQ helicase-like 4
RecQ protein-like 4

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

8

8q24.3

Summary The protein encoded by this gene is a DNA helicase that belongs to the RecQ helicase family. DNA helicases unwind double-stranded DNA into single-stranded DNAs and may modulate chromosome segregation. This gene is predominantly expressed in thymus and testis. Mutations in this gene are associated with Rothmund-Thomson, RAPADILINO and Baller-Gerold syndromes. [provided by RefSeq, Jan 2010].
Disorder MIM:

603780

Disorder Html: Rothmund-Thomson syndrome, 268400 (3); RAPADILINO syndrome, 266280

mRNA and Protein(s)

mRNA Protein Name
NM_004260.3 NP_004251.3 ATP-dependent DNA helicase Q4
XM_017013993.1 XP_016869482.1 ATP-dependent DNA helicase Q4 isoform X3
XM_017013995.1 XP_016869484.1 ATP-dependent DNA helicase Q4 isoform X5
XM_017013997.1 XP_016869486.1 ATP-dependent DNA helicase Q4 isoform X7
XM_017014000.1 XP_016869489.1 ATP-dependent DNA helicase Q4 isoform X11
XM_017013992.1 XP_016869481.1 ATP-dependent DNA helicase Q4 isoform X2
XM_017013998.1 XP_016869487.1 ATP-dependent DNA helicase Q4 isoform X8
XM_017013999.1 XP_016869488.1 ATP-dependent DNA helicase Q4 isoform X9
XM_017014001.1 XP_016869490.1 ATP-dependent DNA helicase Q4 isoform X12
XM_017013991.1 XP_016869480.1 ATP-dependent DNA helicase Q4 isoform X1
XM_017013994.1 XP_016869483.1 ATP-dependent DNA helicase Q4 isoform X4
XM_017013996.1 XP_016869485.1 ATP-dependent DNA helicase Q4 isoform X6
XM_011517384.2 XP_011515686.1 ATP-dependent DNA helicase Q4 isoform X10



Homo sapiens (human) RECQL4 NP_004251.3
Pan troglodytes (chimpanzee) RECQL4 XP_520023.2
Rattus norvegicus (Norway rat) Recql4 NP_001123966.1
Xenopus tropicalis (tropical clawed frog) recql4 NP_001072882.1
Mus musculus (house mouse) Recql4 NP_478121.2
Canis lupus familiaris (dog) RECQL4 XP_005628375.1
Bos taurus (cattle) RECQL4 NP_001091506.1
Danio rerio (zebrafish) LOC567383 XP_001920631.4


Related articles in PubMed

Mitochondrial functions of RECQL4 are required for the prevention of aerobic glycolysis-dependent cell invasion.
Kumari J, Hussain M, De S, Chandra S, Modi P, Tikoo S, Singh A, Sagar C, Sepuri NB, Sengupta S
Journal of cell science129(7)1312-8(2016 Apr)

RECQL4 helicase has oncogenic potential in sporadic breast cancers.
Arora A, Agarwal D, Abdel-Fatah TM, Lu H, Croteau DL, Moseley P, Aleskandarany MA, Green AR, Ball G, Rakha EA, Chan SY, Ellis IO, Wang LL, Zhao Y, Balajee AS, Bohr VA, Madhusudan S
The Journal of pathology238(4)495-501(2016 Mar)

Structural and biochemical characterization of an RNA/DNA binding motif in the N-terminal domain of RecQ4 helicases.
Marino F, Mojumdar A, Zucchelli C, Bhardwaj A, Buratti E, Vindigni A, Musco G, Onesti S
Scientific reports621501(2016 Feb)

RecQL4 is required for the association of Mcm10 and Ctf4 with replication origins in human cells.
Im JS, Park SY, Cho WH, Bae SH, Hurwitz J, Lee JK
Cell cycle (Georgetown, Tex.)14(7)1001-9(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in RECQL4 are responsible for the majority of cases of Rothmund-Thomson syndrome . RECQL4 is important not only in Cancer development but also in the aging process.
Title: Aging in Rothmund-Thomson syndrome and related RECQL4 genetic disorders.

High RecQL4 expression is associated with osteosarcoma.
Title: RecQL4 regulates autophagy and apoptosis in U2OS cells.

High RECQL4 expression is associated with Cisplatin Resistance in Gastric Cancer.
Title: Human Helicase RECQL4 Drives Cisplatin Resistance in Gastric Cancer by Activating an AKT-YB1-MDR1 Signaling Pathway.

this study demonstrates for the first time that, owing to its mitochondrial functions, the accessory mitochondrial replication helicase RECQL4 prevents the invasive step in the neoplastic transformation process.
Title: Mitochondrial functions of RECQL4 are required for the prevention of aerobic glycolysis-dependent cell invasion.

These results reveal novel possible roles of RecQ4 in DNA replication and genome stability.
Title: Structural and biochemical characterization of an RNA/DNA binding motif in the N-terminal domain of RecQ4 helicases.

The following RECQL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RECQL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu98306 XM_017014000.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X14, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu98307 XM_017014001.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X15, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $475.30
$679.00
OHu98297 XM_017013991.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu98298 XM_017013992.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu98299 XM_017013993.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu98300 XM_017013994.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu98301 XM_017013995.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu98302 XM_017013996.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu98303 XM_017013997.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu98304 XM_017013998.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu98305 XM_017013999.1
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu18758 NM_004260.3
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $853.30
$1219.00
OHu62803 XM_011517384.2
Latest version!
Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu98306
Clone ID Related Accession (Same CDS sequence) XM_017014000.1
Accession Version XM_017014000.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X11
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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661
721
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841
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961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGGAAGCAG AAGTGGCGGA AGAAAGGGGA GTGTTTTGGG GGTGGTGGTG CCACAGTCAC 
AACCAAGGAG TCTTGTTTCC TGAACGAGCA GTTCGATCAC TGGGCAGCCC AGTGTCCCCG
GCCAGTGAGG AAGACACAGA TGCTGTTGGG CCTGAGCCAC TGGTTCCTTC ACCACAACCT
GTACCTGAGG TGCCCAGCCT GGACCCCACC GTGCTGCCAC TCTACTCCCT GGGGCCCTCA
GGGCAGTTGG CAGAGACGCC GGCTGAGGTG TTCCAGGCCC TGGAGCAGCT GGGGCACCAA
GCCTTTCGCC CTGGGCAGGA GCGTGCAGTC ATGCGGATCC TGTCTGGCAT CTCCACGCTG
CTGGTGCTGC CTACAGGTGC CGGCAAGTCC CTGTGCTACC AGCTCCCAGC GCTGCTCTAC
AGCCGGCGCA GCCCCTGCCT CACGTTGGTC GTCTCTCCCC TGCTGTCACT CATGGATGAC
CAGGTGTCTG GCCTGCCACC GTGTCTCAAG GCGGCCTGCA TACACTCGGG CATGACCAGG
AAGCAACGGG AATCTGTCCT GCAGAAGATT CGGGCAGCCC AGGTACACGT GCTGATGCTG
ACACCTGAGG CACTGGTGGG GGCGGGAGGC CTCCCTCCAG CCGCACAGCT GCCTCCAGTT
GCTTTTGCCT GCATTGATGA GGCCCACTGC CTCTCCCAGT GGTCCCACAA CTTCCGGCCC
TGCTACCTGC GCGTCTGCAA GGTGCTTCGG GAGCGCATGG GCGTGCACTG CTTCCTGGGC
CTCACAGCCA CAGCCACACG CCGCACTGCC AGTGACGTGG CACAGCACCT GGCTGTGGCT
GAAGAGCCTG ACCTCCACGG GCCAGCCCCA GTTCCCACCA ACCTGCACCT TTCCGTGTCC
ATGGACAGGG ACACAGACCA GGCACTGTTG ACGCTGCTGC AAGGCAAACG TTTTCAAAAC
CTCGATTCCA TTATCATTTA CTGCAACCGG CGCGAGGACA CAGAGCGGAT CGCTGCGCTC
CTCCGAACCT GCCTGCACGC AGCCTGGGTC CCAGGGTCTG GAGGTGCGGC ATGGACAGAG
CTGGTGTCCC CGTGGACCCA CCTTGGGCAC ACATGGTCCC ATCCCACTGA CCATCTGCCT
GTCTTCCCCA AAGGTCGTGC CCCCAAAACC ACAGCCGAGG CCTACCACGC GGGCATGTGC
AGCCGGGAAC GGCGGCGGGT ACAGCGAGCC TTCATGCAGG GCCAGTTGCG GGTGGTGGTG
GCCACGGTGG CCTTTGGGAT GGGGCTGGAC CGGCCAGATG TGCGGGCTGT GCTGCATCTG
GGGCTGCCCC CAAGCTTCGA GAGCTACGTG CAGGCCGTGG GCCGGGCCGG GCGTGACGGG
CAGCCTGCCC ACTGCCACCT CTTCCTGCAG CCCCAGGGCG AAGACCTGCG AGAGCTGCGC
AGACATGTGC ACGCCGACAG CACGGACTTC CTGGCTGTGA AGAGGCTGGT ACAGCGCGTG
TTCCCAGCCT GCACCTGCAC CTGCACCAGG CCGCCCTCGG AGCAGGAAGG GGCCGTGGGT
GGGGAGAGGC CTGTGCCCAA GTACCCCCCT CAAGAGGCTG AGCAGCTTAG CCACCAAGCA
GCCCCAGGAC CCAGAAGGGT CTGCATGGGC CATGAGCGGG CACTCCCAAT ACAGCTTACC
GTACAGGCTT TGGACATGCC GGAGGAGGGT GAGGAACCTG GGGTAAGCCA CAGGGGTGTG
GAGGGGCTGT CCCCGCGTCC GCTGAGCCCT GCTCTGCCCC CAGCCATCGA GACTTTGCTG
TGCTACCTGG AGCTGCACCC ACACCACTGG CTGGAGCTGC TGGCGACCAC CTATACCCAT
TGCCGTCTGA ACTGCCCTGG GGGCCCTGCC CAGCTCCAGG CCCTGGCCCA CAGGTGTCCC
CCTTTGGCTG TGTGCTTGGC CCAGCAGCTG CCTGAGGACC CAGGGCAAGG CAGCAGCTCC
GTGGAGTTTG ACATGGTCAA GCTGGTGGAC TCCATGGGCT GGGAGCTGGC CTCTGTGCGG
CGGGCTCTCT GCCAGCTGCA GTGGGACCAC GAGCCCAGGA CAGGTGTGCG GCGTGGGACA
GGGGTGCTTG TGGAGTTCAG TGAGCTGGCC TTCCACCTTC GCAGCCCGGG GGACTTGACC
GCTGAGGAGA AGGACCAGAT ATGTGACTTC CTCTATGGCC GTGTGCAGGC CCGGGAGCGC
CAGGCCCTGG CCCGTCTGCG CAGAACCTTC CAGGCCTTTC ACAGCGTAGC CTTCCCCAGC
TGCGGGCCCT GCCTGGAGCA GCAGGATGAG GAGCGCAGCA CCAGGCTCAA GGACCTGCTC
GGCCGCTACT TTGAGGAAGA GGAAGGGCAG GAGCCGGGAG GCATGGAGGA CGCACAGGGC
CCCGAGCCAG GGCAGGCCAG AGTGAGTGCA GTAAGGCCAG GCAGCTCATC GGGGTTGCAG
GTTCCCTGGG CTGCATGGGG CTTGCTCTGT GGATGCAGTG CCACGGGAGC TCAGAGGAAG
CCTGATGTGC CTGTCCACAC AGCTCCAGGA TTGGGAGGAC CAGGTCCGCT GCGACATCCG
CCAGTTCCTG TCCCTGAGGC CAGAGGAGAA GTTCTCCAGC AGGGCTGTGG CCCGCATCTT
CCACGGCATC GGAAGCCCCT GCTACCCGGC CCAGGTGTAC GGGCAGGACC GACGCTTCTG
GAGAAAATAC CTGCACCTGA GCTTCCATGC CCTGGTGGGC CTGGCCACGG AAGAGCTCCT
GCAGGTGGCC CGCTGACTGC ACTGCATTGG GGGATGTCGG GTAGAGCTGG GGTTGTCAGA
GGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869489.1
CDS96..2921
Translation

Target ORF information:

RefSeq Version XM_017014000.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X14, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017014000.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
ATGGAAGCAG AAGTGGCGGA AGAAAGGGGA GTGTTTTGGG GGTGGTGGTG CCACAGTCAC 
AACCAAGGAG TCTTGTTTCC TGAACGAGCA GTTCGATCAC TGGGCAGCCC AGTGTCCCCG
GCCAGTGAGG AAGACACAGA TGCTGTTGGG CCTGAGCCAC TGGTTCCTTC ACCACAACCT
GTACCTGAGG TGCCCAGCCT GGACCCCACC GTGCTGCCAC TCTACTCCCT GGGGCCCTCA
GGGCAGTTGG CAGAGACGCC GGCTGAGGTG TTCCAGGCCC TGGAGCAGCT GGGGCACCAA
GCCTTTCGCC CTGGGCAGGA GCGTGCAGTC ATGCGGATCC TGTCTGGCAT CTCCACGCTG
CTGGTGCTGC CTACAGGTGC CGGCAAGTCC CTGTGCTACC AGCTCCCAGC GCTGCTCTAC
AGCCGGCGCA GCCCCTGCCT CACGTTGGTC GTCTCTCCCC TGCTGTCACT CATGGATGAC
CAGGTGTCTG GCCTGCCACC GTGTCTCAAG GCGGCCTGCA TACACTCGGG CATGACCAGG
AAGCAACGGG AATCTGTCCT GCAGAAGATT CGGGCAGCCC AGGTACACGT GCTGATGCTG
ACACCTGAGG CACTGGTGGG GGCGGGAGGC CTCCCTCCAG CCGCACAGCT GCCTCCAGTT
GCTTTTGCCT GCATTGATGA GGCCCACTGC CTCTCCCAGT GGTCCCACAA CTTCCGGCCC
TGCTACCTGC GCGTCTGCAA GGTGCTTCGG GAGCGCATGG GCGTGCACTG CTTCCTGGGC
CTCACAGCCA CAGCCACACG CCGCACTGCC AGTGACGTGG CACAGCACCT GGCTGTGGCT
GAAGAGCCTG ACCTCCACGG GCCAGCCCCA GTTCCCACCA ACCTGCACCT TTCCGTGTCC
ATGGACAGGG ACACAGACCA GGCACTGTTG ACGCTGCTGC AAGGCAAACG TTTTCAAAAC
CTCGATTCCA TTATCATTTA CTGCAACCGG CGCGAGGACA CAGAGCGGAT CGCTGCGCTC
CTCCGAACCT GCCTGCACGC AGCCTGGGTC CCAGGGTCTG GAGGTGCGGC ATGGACAGAG
CTGGTGTCCC CGTGGACCCA CCTTGGGCAC ACATGGTCCC ATCCCACTGA CCATCTGCCT
GTCTTCCCCA AAGGTCGTGC CCCCAAAACC ACAGCCGAGG CCTACCACGC GGGCATGTGC
AGCCGGGAAC GGCGGCGGGT ACAGCGAGCC TTCATGCAGG GCCAGTTGCG GGTGGTGGTG
GCCACGGTGG CCTTTGGGAT GGGGCTGGAC CGGCCAGATG TGCGGGCTGT GCTGCATCTG
GGGCTGCCCC CAAGCTTCGA GAGCTACGTG CAGGCCGTGG GCCGGGCCGG GCGTGACGGG
CAGCCTGCCC ACTGCCACCT CTTCCTGCAG CCCCAGGGCG AAGACCTGCG AGAGCTGCGC
AGACATGTGC ACGCCGACAG CACGGACTTC CTGGCTGTGA AGAGGCTGGT ACAGCGCGTG
TTCCCAGCCT GCACCTGCAC CTGCACCAGG CCGCCCTCGG AGCAGGAAGG GGCCGTGGGT
GGGGAGAGGC CTGTGCCCAA GTACCCCCCT CAAGAGGCTG AGCAGCTTAG CCACCAAGCA
GCCCCAGGAC CCAGAAGGGT CTGCATGGGC CATGAGCGGG CACTCCCAAT ACAGCTTACC
GTACAGGCTT TGGACATGCC GGAGGAGGGT GAGGAACCTG GGGTAAGCCA CAGGGGTGTG
GAGGGGCTGT CCCCGCGTCC GCTGAGCCCT GCTCTGCCCC CAGCCATCGA GACTTTGCTG
TGCTACCTGG AGCTGCACCC ACACCACTGG CTGGAGCTGC TGGCGACCAC CTATACCCAT
TGCCGTCTGA ACTGCCCTGG GGGCCCTGCC CAGCTCCAGG CCCTGGCCCA CAGGTGTCCC
CCTTTGGCTG TGTGCTTGGC CCAGCAGCTG CCTGAGGACC CAGGGCAAGG CAGCAGCTCC
GTGGAGTTTG ACATGGTCAA GCTGGTGGAC TCCATGGGCT GGGAGCTGGC CTCTGTGCGG
CGGGCTCTCT GCCAGCTGCA GTGGGACCAC GAGCCCAGGA CAGGTGTGCG GCGTGGGACA
GGGGTGCTTG TGGAGTTCAG TGAGCTGGCC TTCCACCTTC GCAGCCCGGG GGACTTGACC
GCTGAGGAGA AGGACCAGAT ATGTGACTTC CTCTATGGCC GTGTGCAGGC CCGGGAGCGC
CAGGCCCTGG CCCGTCTGCG CAGAACCTTC CAGGCCTTTC ACAGCGTAGC CTTCCCCAGC
TGCGGGCCCT GCCTGGAGCA GCAGGATGAG GAGCGCAGCA CCAGGCTCAA GGACCTGCTC
GGCCGCTACT TTGAGGAAGA GGAAGGGCAG GAGCCGGGAG GCATGGAGGA CGCACAGGGC
CCCGAGCCAG GGCAGGCCAG AGTGAGTGCA GTAAGGCCAG GCAGCTCATC GGGGTTGCAG
GTTCCCTGGG CTGCATGGGG CTTGCTCTGT GGATGCAGTG CCACGGGAGC TCAGAGGAAG
CCTGATGTGC CTGTCCACAC AGCTCCAGGA TTGGGAGGAC CAGGTCCGCT GCGACATCCG
CCAGTTCCTG TCCCTGAGGC CAGAGGAGAA GTTCTCCAGC AGGGCTGTGG CCCGCATCTT
CCACGGCATC GGAAGCCCCT GCTACCCGGC CCAGGTGTAC GGGCAGGACC GACGCTTCTG
GAGAAAATAC CTGCACCTGA GCTTCCATGC CCTGGTGGGC CTGGCCACGG AAGAGCTCCT
GCAGGTGGCC CGCTGACTGC ACTGCATTGG GGGATGTCGG GTAGAGCTGG GGTTGTCAGA
GGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98307
Clone ID Related Accession (Same CDS sequence) XM_017014001.1
Accession Version XM_017014001.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X12
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGAAGCAG AAGTGGCGGA AGAAAGGGGA GTGTTTTGGG GGTGGTGGTG CCACAGTCAC 
AACCAAGGAG TCTTGTTTCC TGAACGAGCA GTTCGATCAC TGGGCAGCCC AGTGTCCCCG
GCCAGTGAGG AAGACACAGA TGCTGTTGGG CCTGAGCCAC TGGTTCCTTC ACCACAACCT
GTACCTGAGG TGCCCAGCCT GGACCCCACC GTGCTGCCAC TCTACTCCCT GGGGCCCTCA
GGGCAGTTGG CAGAGACGCC GGCTGAGGTG TTCCAGGCCC TGGAGCAGCT GGGGCACCAA
GCCTTTCGCC CTGGGCAGGA GCGTGCAGTC ATGCGGATCC TGTCTGGCAT CTCCACGCTG
CTGGTGCTGC CTACAGGTGC CGGCAAGTCC CTGTGCTACC AGCTCCCAGC GCTGCTCTAC
AGCCGGCGCA GCCCCTGCCT CACGTTGGTC GTCTCTCCCC TGCTGTCACT CATGGATGAC
CAGGTGTCTG GCCTGCCACC GTGTCTCAAG GCGGCCTGCA TACACTCGGG CATGACCAGG
AAGCAACGGG AATCTGTCCT GCAGAAGATT CGGGCAGCCC AGGTACACGT GCTGATGCTG
ACACCTGAGG CACTGGTGGG GGCGGGAGGC CTCCCTCCAG CCGCACAGCT GCCTCCAGTT
GCTTTTGCCT GCATTGATGA GGCCCACTGC CTCTCCCAGT GGTCCCACAA CTTCCGGCCC
TGCTACCTGC GCGTCTGCAA GGTGCTTCGG GAGCGCATGG GCGTGCACTG CTTCCTGGGC
CTCACAGCCA CAGCCACACG CCGCACTGCC AGTGACGTGG CACAGCACCT GGCTGTGGCT
GAAGAGCCTG ACCTCCACGG GCCAGCCCCA GTTCCCACCA ACCTGCACCT TTCCGTGTCC
ATGGACAGGG ACACAGACCA GGCACTGTTG ACGCTGCTGC AAGGCAAACG TTTTCAAAAC
CTCGATTCCA TTATCATTTA CTGCAACCGG CGCGAGGACA CAGAGCGGAT CGCTGCGCTC
CTCCGAACCT GCCTGCACGC AGCCTGGGTC CCAGGGTCTG GAGGTCGTGC CCCCAAAACC
ACAGCCGAGG CCTACCACGC GGGCATGTGC AGCCGGGAAC GGCGGCGGGT ACAGCGAGCC
TTCATGCAGG GCCAGTTGCG GGTGGTGGTG GCCACGGTGG CCTTTGGGAT GGGGCTGGAC
CGGCCAGATG TGCGGGCTGT GCTGCATCTG GGGCTGCCCC CAAGCTTCGA GAGCTACGTG
CAGGCCGTGG GCCGGGCCGG GCGTGACGGG CAGCCTGCCC ACTGCCACCT CTTCCTGCAG
CCCCAGGGCG AAGACCTGCG AGAGCTGCGC AGACATGTGC ACGCCGACAG CACGGACTTC
CTGGCTGTGA AGAGGCTGGT ACAGCGCGTG TTCCCAGCCT GCACCTGCAC CTGCACCAGG
CCGCCCTCGG AGCAGGAAGG GGCCGTGGGT GGGGAGAGGC CTGTGCCCAA GTACCCCCCT
CAAGAGGCTG AGCAGCTTAG CCACCAAGCA GCCCCAGGAC CCAGAAGGGT CTGCATGGGC
CATGAGCGGG CACTCCCAAT ACAGCTTACC GTACAGGCTT TGGACATGCC GGAGGAGGGT
GAGGAACCTG GGGTAAGCCA CAGGGGTGTG GAGGGGCTGT CCCCGCGTCC GCTGAGCCCT
GCTCTGCCCC CAGCCATCGA GACTTTGCTG TGCTACCTGG AGCTGCACCC ACACCACTGG
CTGGAGCTGC TGGCGACCAC CTATACCCAT TGCCGTCTGA ACTGCCCTGG GGGCCCTGCC
CAGCTCCAGG CCCTGGCCCA CAGGTGTCCC CCTTTGGCTG TGTGCTTGGC CCAGCAGCTG
CCTGAGGACC CAGGGCAAGG CAGCAGCTCC GTGGAGTTTG ACATGGTCAA GCTGGTGGAC
TCCATGGGCT GGGAGCTGGC CTCTGTGCGG CGGGCTCTCT GCCAGCTGCA GTGGGACCAC
GAGCCCAGGA CAGGTGTGCG GCGTGGGACA GGGGTGCTTG TGGAGTTCAG TGAGCTGGCC
TTCCACCTTC GCAGCCCGGG GGACTTGACC GCTGAGGAGA AGGACCAGAT ATGTGACTTC
CTCTATGGCC GTGTGCAGGC CCGGGAGCGC CAGGCCCTGG CCCGTCTGCG CAGAACCTTC
CAGGCCTTTC ACAGCGTAGC CTTCCCCAGC TGCGGGCCCT GCCTGGAGCA GCAGGATGAG
GAGCGCAGCA CCAGGCTCAA GGACCTGCTC GGCCGCTACT TTGAGGAAGA GGAAGGGCAG
GAGCCGGGAG GCATGGAGGA CGCACAGGGC CCCGAGCCAG GGCAGGCCAG AGTGAGTGCA
GTAAGGCCAG GCAGCTCATC GGGGTTGCAG GTTCCCTGGG CTGCATGGGG CTTGCTCTGT
GGATGCAGTG CCACGGGAGC TCAGAGGAAG CCTGATGTGC CTGTCCACAC AGCTCCAGGA
TTGGGAGGAC CAGGTCCGCT GCGACATCCG CCAGTTCCTG TCCCTGAGGC CAGAGGAGAA
GTTCTCCAGC AGGGCTGTGG CCCGCATCTT CCACGGCATC GGAAGCCCCT GCTACCCGGC
CCAGGTGTAC GGGCAGGACC GACGCTTCTG GAGAAAATAC CTGCACCTGA GCTTCCATGC
CCTGGTGGGC CTGGCCACGG AAGAGCTCCT GCAGGTGGCC CGCTGACTGC ACTGCATTGG
GGGATGTCGG GTAGAGCTGG GGTTGTCAGA GGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869490.1
CDS1276..4011
Translation

Target ORF information:

RefSeq Version XM_017014001.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X15, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017014001.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
ATGGAAGCAG AAGTGGCGGA AGAAAGGGGA GTGTTTTGGG GGTGGTGGTG CCACAGTCAC 
AACCAAGGAG TCTTGTTTCC TGAACGAGCA GTTCGATCAC TGGGCAGCCC AGTGTCCCCG
GCCAGTGAGG AAGACACAGA TGCTGTTGGG CCTGAGCCAC TGGTTCCTTC ACCACAACCT
GTACCTGAGG TGCCCAGCCT GGACCCCACC GTGCTGCCAC TCTACTCCCT GGGGCCCTCA
GGGCAGTTGG CAGAGACGCC GGCTGAGGTG TTCCAGGCCC TGGAGCAGCT GGGGCACCAA
GCCTTTCGCC CTGGGCAGGA GCGTGCAGTC ATGCGGATCC TGTCTGGCAT CTCCACGCTG
CTGGTGCTGC CTACAGGTGC CGGCAAGTCC CTGTGCTACC AGCTCCCAGC GCTGCTCTAC
AGCCGGCGCA GCCCCTGCCT CACGTTGGTC GTCTCTCCCC TGCTGTCACT CATGGATGAC
CAGGTGTCTG GCCTGCCACC GTGTCTCAAG GCGGCCTGCA TACACTCGGG CATGACCAGG
AAGCAACGGG AATCTGTCCT GCAGAAGATT CGGGCAGCCC AGGTACACGT GCTGATGCTG
ACACCTGAGG CACTGGTGGG GGCGGGAGGC CTCCCTCCAG CCGCACAGCT GCCTCCAGTT
GCTTTTGCCT GCATTGATGA GGCCCACTGC CTCTCCCAGT GGTCCCACAA CTTCCGGCCC
TGCTACCTGC GCGTCTGCAA GGTGCTTCGG GAGCGCATGG GCGTGCACTG CTTCCTGGGC
CTCACAGCCA CAGCCACACG CCGCACTGCC AGTGACGTGG CACAGCACCT GGCTGTGGCT
GAAGAGCCTG ACCTCCACGG GCCAGCCCCA GTTCCCACCA ACCTGCACCT TTCCGTGTCC
ATGGACAGGG ACACAGACCA GGCACTGTTG ACGCTGCTGC AAGGCAAACG TTTTCAAAAC
CTCGATTCCA TTATCATTTA CTGCAACCGG CGCGAGGACA CAGAGCGGAT CGCTGCGCTC
CTCCGAACCT GCCTGCACGC AGCCTGGGTC CCAGGGTCTG GAGGTCGTGC CCCCAAAACC
ACAGCCGAGG CCTACCACGC GGGCATGTGC AGCCGGGAAC GGCGGCGGGT ACAGCGAGCC
TTCATGCAGG GCCAGTTGCG GGTGGTGGTG GCCACGGTGG CCTTTGGGAT GGGGCTGGAC
CGGCCAGATG TGCGGGCTGT GCTGCATCTG GGGCTGCCCC CAAGCTTCGA GAGCTACGTG
CAGGCCGTGG GCCGGGCCGG GCGTGACGGG CAGCCTGCCC ACTGCCACCT CTTCCTGCAG
CCCCAGGGCG AAGACCTGCG AGAGCTGCGC AGACATGTGC ACGCCGACAG CACGGACTTC
CTGGCTGTGA AGAGGCTGGT ACAGCGCGTG TTCCCAGCCT GCACCTGCAC CTGCACCAGG
CCGCCCTCGG AGCAGGAAGG GGCCGTGGGT GGGGAGAGGC CTGTGCCCAA GTACCCCCCT
CAAGAGGCTG AGCAGCTTAG CCACCAAGCA GCCCCAGGAC CCAGAAGGGT CTGCATGGGC
CATGAGCGGG CACTCCCAAT ACAGCTTACC GTACAGGCTT TGGACATGCC GGAGGAGGGT
GAGGAACCTG GGGTAAGCCA CAGGGGTGTG GAGGGGCTGT CCCCGCGTCC GCTGAGCCCT
GCTCTGCCCC CAGCCATCGA GACTTTGCTG TGCTACCTGG AGCTGCACCC ACACCACTGG
CTGGAGCTGC TGGCGACCAC CTATACCCAT TGCCGTCTGA ACTGCCCTGG GGGCCCTGCC
CAGCTCCAGG CCCTGGCCCA CAGGTGTCCC CCTTTGGCTG TGTGCTTGGC CCAGCAGCTG
CCTGAGGACC CAGGGCAAGG CAGCAGCTCC GTGGAGTTTG ACATGGTCAA GCTGGTGGAC
TCCATGGGCT GGGAGCTGGC CTCTGTGCGG CGGGCTCTCT GCCAGCTGCA GTGGGACCAC
GAGCCCAGGA CAGGTGTGCG GCGTGGGACA GGGGTGCTTG TGGAGTTCAG TGAGCTGGCC
TTCCACCTTC GCAGCCCGGG GGACTTGACC GCTGAGGAGA AGGACCAGAT ATGTGACTTC
CTCTATGGCC GTGTGCAGGC CCGGGAGCGC CAGGCCCTGG CCCGTCTGCG CAGAACCTTC
CAGGCCTTTC ACAGCGTAGC CTTCCCCAGC TGCGGGCCCT GCCTGGAGCA GCAGGATGAG
GAGCGCAGCA CCAGGCTCAA GGACCTGCTC GGCCGCTACT TTGAGGAAGA GGAAGGGCAG
GAGCCGGGAG GCATGGAGGA CGCACAGGGC CCCGAGCCAG GGCAGGCCAG AGTGAGTGCA
GTAAGGCCAG GCAGCTCATC GGGGTTGCAG GTTCCCTGGG CTGCATGGGG CTTGCTCTGT
GGATGCAGTG CCACGGGAGC TCAGAGGAAG CCTGATGTGC CTGTCCACAC AGCTCCAGGA
TTGGGAGGAC CAGGTCCGCT GCGACATCCG CCAGTTCCTG TCCCTGAGGC CAGAGGAGAA
GTTCTCCAGC AGGGCTGTGG CCCGCATCTT CCACGGCATC GGAAGCCCCT GCTACCCGGC
CCAGGTGTAC GGGCAGGACC GACGCTTCTG GAGAAAATAC CTGCACCTGA GCTTCCATGC
CCTGGTGGGC CTGGCCACGG AAGAGCTCCT GCAGGTGGCC CGCTGACTGC ACTGCATTGG
GGGATGTCGG GTAGAGCTGG GGTTGTCAGA GGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98297
Clone ID Related Accession (Same CDS sequence) XM_017013991.1
Accession Version XM_017013991.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGTACA GCGAGCCTTC ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC
ACGGTGGCCT TTGGGATGGG GCTGGACCGG CCAGATGTGC GGGCTGTGCT GCATCTGGGG
CTGCCCCCAA GCTTCGAGAG CTACGTGCAG GCCGTGGGCC GGGCCGGGCG TGACGGGCAG
CCTGCCCACT GCCACCTCTT CCTGCAGCCC CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA
CATGTGCACG CCGACAGCAC GGACTTCCTG GCTGTGAAGA GGCTGGTACA GCGCGTGTTC
CCAGCCTGCA CCTGCACCTG CACCAGGCCG CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG
GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC
CCAGGACCCA GAAGGGTCTG CATGGGCCAT GAGCGGGCAC TCCCAATACA GCTTACCGTA
CAGGCTTTGG ACATGCCGGA GGAGGGTGAG GAACCTGGGG TAAGCCACAG GGGTGTGGAG
GGGCTGTCCC CGCGTCCGCT GAGCCCTGCT CTGCCCCCAG CCATCGAGAC TTTGCTGTGC
TACCTGGAGC TGCACCCACA CCACTGGCTG GAGCTGCTGG CGACCACCTA TACCCATTGC
CGTCTGAACT GCCCTGGGGG CCCTGCCCAG CTCCAGGCCC TGGCCCACAG GTGTCCCCCT
TTGGCTGTGT GCTTGGCCCA GCAGCTGCCT GAGGACCCAG GGCAAGGCAG CAGCTCCGTG
GAGTTTGACA TGGTCAAGCT GGTGGACTCC ATGGGCTGGG AGCTGGCCTC TGTGCGGCGG
GCTCTCTGCC AGCTGCAGTG GGACCACGAG CCCAGGACAG GTGTGCGGCG TGGGACAGGG
GTGCTTGTGG AGTTCAGTGA GCTGGCCTTC CACCTTCGCA GCCCGGGGGA CTTGACCGCT
GAGGAGAAGG ACCAGATATG TGACTTCCTC TATGGCCGTG TGCAGGCCCG GGAGCGCCAG
GCCCTGGCCC GTCTGCGCAG AACCTTCCAG GCCTTTCACA GCGTAGCCTT CCCCAGCTGC
GGGCCCTGCC TGGAGCAGCA GGATGAGGAG CGCAGCACCA GGCTCAAGGA CCTGCTCGGC
CGCTACTTTG AGGAAGAGGA AGGGCAGGAG CCGGGAGGCA TGGAGGACGC ACAGGGCCCC
GAGCCAGGGC AGGCCAGAGT GAGTGCAGTA AGGCCAGGCA GCTCATCGGG GTTGCAGGTT
CCCTGGGCTG CATGGGGCTT GCTCTGTGGA TGCAGTGCCA CGGGAGCTCA GAGGAAGCCT
GATGTGCCTG TCCACACAGC TCCAGGATTG GGAGGACCAG GTCCGCTGCG ACATCCGCCA
GTTCCTGTCC CTGAGGCCAG AGGAGAAGTT CTCCAGCAGG GCTGTGGCCC GCATCTTCCA
CGGCATCGGA AGCCCCTGCT ACCCGGCCCA GGTGTACGGG CAGGACCGAC GCTTCTGGAG
AAAATACCTG CACCTGAGCT TCCATGCCCT GGTGGGCCTG GCCACGGAAG AGCTCCTGCA
GGTGGCCCGC TGACTGCACT GCATTGGGGG ATGTCGGGTA GAGCTGGGGT TGTCAGAGGC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869480.1
CDS48..4010
Translation

Target ORF information:

RefSeq Version XM_017013991.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013991.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGTACA GCGAGCCTTC ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC
ACGGTGGCCT TTGGGATGGG GCTGGACCGG CCAGATGTGC GGGCTGTGCT GCATCTGGGG
CTGCCCCCAA GCTTCGAGAG CTACGTGCAG GCCGTGGGCC GGGCCGGGCG TGACGGGCAG
CCTGCCCACT GCCACCTCTT CCTGCAGCCC CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA
CATGTGCACG CCGACAGCAC GGACTTCCTG GCTGTGAAGA GGCTGGTACA GCGCGTGTTC
CCAGCCTGCA CCTGCACCTG CACCAGGCCG CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG
GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC
CCAGGACCCA GAAGGGTCTG CATGGGCCAT GAGCGGGCAC TCCCAATACA GCTTACCGTA
CAGGCTTTGG ACATGCCGGA GGAGGGTGAG GAACCTGGGG TAAGCCACAG GGGTGTGGAG
GGGCTGTCCC CGCGTCCGCT GAGCCCTGCT CTGCCCCCAG CCATCGAGAC TTTGCTGTGC
TACCTGGAGC TGCACCCACA CCACTGGCTG GAGCTGCTGG CGACCACCTA TACCCATTGC
CGTCTGAACT GCCCTGGGGG CCCTGCCCAG CTCCAGGCCC TGGCCCACAG GTGTCCCCCT
TTGGCTGTGT GCTTGGCCCA GCAGCTGCCT GAGGACCCAG GGCAAGGCAG CAGCTCCGTG
GAGTTTGACA TGGTCAAGCT GGTGGACTCC ATGGGCTGGG AGCTGGCCTC TGTGCGGCGG
GCTCTCTGCC AGCTGCAGTG GGACCACGAG CCCAGGACAG GTGTGCGGCG TGGGACAGGG
GTGCTTGTGG AGTTCAGTGA GCTGGCCTTC CACCTTCGCA GCCCGGGGGA CTTGACCGCT
GAGGAGAAGG ACCAGATATG TGACTTCCTC TATGGCCGTG TGCAGGCCCG GGAGCGCCAG
GCCCTGGCCC GTCTGCGCAG AACCTTCCAG GCCTTTCACA GCGTAGCCTT CCCCAGCTGC
GGGCCCTGCC TGGAGCAGCA GGATGAGGAG CGCAGCACCA GGCTCAAGGA CCTGCTCGGC
CGCTACTTTG AGGAAGAGGA AGGGCAGGAG CCGGGAGGCA TGGAGGACGC ACAGGGCCCC
GAGCCAGGGC AGGCCAGAGT GAGTGCAGTA AGGCCAGGCA GCTCATCGGG GTTGCAGGTT
CCCTGGGCTG CATGGGGCTT GCTCTGTGGA TGCAGTGCCA CGGGAGCTCA GAGGAAGCCT
GATGTGCCTG TCCACACAGC TCCAGGATTG GGAGGACCAG GTCCGCTGCG ACATCCGCCA
GTTCCTGTCC CTGAGGCCAG AGGAGAAGTT CTCCAGCAGG GCTGTGGCCC GCATCTTCCA
CGGCATCGGA AGCCCCTGCT ACCCGGCCCA GGTGTACGGG CAGGACCGAC GCTTCTGGAG
AAAATACCTG CACCTGAGCT TCCATGCCCT GGTGGGCCTG GCCACGGAAG AGCTCCTGCA
GGTGGCCCGC TGACTGCACT GCATTGGGGG ATGTCGGGTA GAGCTGGGGT TGTCAGAGGC
TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98298
Clone ID Related Accession (Same CDS sequence) XM_017013992.1
Accession Version XM_017013992.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGTACA GCGAGCCTTC ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC
ACGGTGGCCT TTGGGATGGG GCTGGACCGG CCAGATGTGC GGGCTGTGCT GCATCTGGGG
CTGCCCCCAA GCTTCGAGAG CTACGTGCAG GCCGTGGGCC GGGCCGGGCG TGACGGGCAG
CCTGCCCACT GCCACCTCTT CCTGCAGCCC CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA
CATGTGCACG CCGACAGCAC GGACTTCCTG GCTGTGAAGA GGCTGGTACA GCGCGTGTTC
CCAGCCTGCA CCTGCACCTG CACCAGGCCG CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG
GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC
CCAGGACCCA GAAGGGTCTG CATGGGCCAT GAGCGGGCAC TCCCAATACA GCTTACCGTA
CAGGCTTTGG ACATGCCGGA GGAGGCCATC GAGACTTTGC TGTGCTACCT GGAGCTGCAC
CCACACCACT GGCTGGAGCT GCTGGCGACC ACCTATACCC ATTGCCGTCT GAACTGCCCT
GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC CACAGGTGTC CCCCTTTGGC TGTGTGCTTG
GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA GGCAGCAGCT CCGTGGAGTT TGACATGGTC
AAGCTGGTGG ACTCCATGGG CTGGGAGCTG GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG
CAGTGGGACC ACGAGCCCAG GACAGGTGTG CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC
AGTGAGCTGG CCTTCCACCT TCGCAGCCCG GGGGACTTGA CCGCTGAGGA GAAGGACCAG
ATATGTGACT TCCTCTATGG CCGTGTGCAG GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG
CGCAGAACCT TCCAGGCCTT TCACAGCGTA GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG
CAGCAGGATG AGGAGCGCAG CACCAGGCTC AAGGACCTGC TCGGCCGCTA CTTTGAGGAA
GAGGAAGGGC AGGAGCCGGG AGGCATGGAG GACGCACAGG GCCCCGAGCC AGGGCAGGCC
AGAGTGAGTG CAGTAAGGCC AGGCAGCTCA TCGGGGTTGC AGGTTCCCTG GGCTGCATGG
GGCTTGCTCT GTGGATGCAG TGCCACGGGA GCTCAGAGGA AGCCTGATGT GCCTGTCCAC
ACAGCTCCAG GATTGGGAGG ACCAGGTCCG CTGCGACATC CGCCAGTTCC TGTCCCTGAG
GCCAGAGGAG AAGTTCTCCA GCAGGGCTGT GGCCCGCATC TTCCACGGCA TCGGAAGCCC
CTGCTACCCG GCCCAGGTGT ACGGGCAGGA CCGACGCTTC TGGAGAAAAT ACCTGCACCT
GAGCTTCCAT GCCCTGGTGG GCCTGGCCAC GGAAGAGCTC CTGCAGGTGG CCCGCTGACT
GCACTGCATT GGGGGATGTC GGGTAGAGCT GGGGTTGTCA GAGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869481.1
CDS47..3934
Translation

Target ORF information:

RefSeq Version XM_017013992.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013992.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGTACA GCGAGCCTTC ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC
ACGGTGGCCT TTGGGATGGG GCTGGACCGG CCAGATGTGC GGGCTGTGCT GCATCTGGGG
CTGCCCCCAA GCTTCGAGAG CTACGTGCAG GCCGTGGGCC GGGCCGGGCG TGACGGGCAG
CCTGCCCACT GCCACCTCTT CCTGCAGCCC CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA
CATGTGCACG CCGACAGCAC GGACTTCCTG GCTGTGAAGA GGCTGGTACA GCGCGTGTTC
CCAGCCTGCA CCTGCACCTG CACCAGGCCG CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG
GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC
CCAGGACCCA GAAGGGTCTG CATGGGCCAT GAGCGGGCAC TCCCAATACA GCTTACCGTA
CAGGCTTTGG ACATGCCGGA GGAGGCCATC GAGACTTTGC TGTGCTACCT GGAGCTGCAC
CCACACCACT GGCTGGAGCT GCTGGCGACC ACCTATACCC ATTGCCGTCT GAACTGCCCT
GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC CACAGGTGTC CCCCTTTGGC TGTGTGCTTG
GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA GGCAGCAGCT CCGTGGAGTT TGACATGGTC
AAGCTGGTGG ACTCCATGGG CTGGGAGCTG GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG
CAGTGGGACC ACGAGCCCAG GACAGGTGTG CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC
AGTGAGCTGG CCTTCCACCT TCGCAGCCCG GGGGACTTGA CCGCTGAGGA GAAGGACCAG
ATATGTGACT TCCTCTATGG CCGTGTGCAG GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG
CGCAGAACCT TCCAGGCCTT TCACAGCGTA GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG
CAGCAGGATG AGGAGCGCAG CACCAGGCTC AAGGACCTGC TCGGCCGCTA CTTTGAGGAA
GAGGAAGGGC AGGAGCCGGG AGGCATGGAG GACGCACAGG GCCCCGAGCC AGGGCAGGCC
AGAGTGAGTG CAGTAAGGCC AGGCAGCTCA TCGGGGTTGC AGGTTCCCTG GGCTGCATGG
GGCTTGCTCT GTGGATGCAG TGCCACGGGA GCTCAGAGGA AGCCTGATGT GCCTGTCCAC
ACAGCTCCAG GATTGGGAGG ACCAGGTCCG CTGCGACATC CGCCAGTTCC TGTCCCTGAG
GCCAGAGGAG AAGTTCTCCA GCAGGGCTGT GGCCCGCATC TTCCACGGCA TCGGAAGCCC
CTGCTACCCG GCCCAGGTGT ACGGGCAGGA CCGACGCTTC TGGAGAAAAT ACCTGCACCT
GAGCTTCCAT GCCCTGGTGG GCCTGGCCAC GGAAGAGCTC CTGCAGGTGG CCCGCTGACT
GCACTGCATT GGGGGATGTC GGGTAGAGCT GGGGTTGTCA GAGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98299
Clone ID Related Accession (Same CDS sequence) XM_017013993.1
Accession Version XM_017013993.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGGTGAG
GAACCTGGGG TAAGCCACAG GGGTGTGGAG GGGCTGTCCC CGCGTCCGCT GAGCCCTGCT
CTGCCCCCAG CCATCGAGAC TTTGCTGTGC TACCTGGAGC TGCACCCACA CCACTGGCTG
GAGCTGCTGG CGACCACCTA TACCCATTGC CGTCTGAACT GCCCTGGGGG CCCTGCCCAG
CTCCAGGCCC TGGCCCACAG GTGTCCCCCT TTGGCTGTGT GCTTGGCCCA GCAGCTGCCT
GAGGACCCAG GGCAAGGCAG CAGCTCCGTG GAGTTTGACA TGGTCAAGCT GGTGGACTCC
ATGGGCTGGG AGCTGGCCTC TGTGCGGCGG GCTCTCTGCC AGCTGCAGTG GGACCACGAG
CCCAGGACAG GTGTGCGGCG TGGGACAGGG GTGCTTGTGG AGTTCAGTGA GCTGGCCTTC
CACCTTCGCA GCCCGGGGGA CTTGACCGCT GAGGAGAAGG ACCAGATATG TGACTTCCTC
TATGGCCGTG TGCAGGCCCG GGAGCGCCAG GCCCTGGCCC GTCTGCGCAG AACCTTCCAG
GCCTTTCACA GCGTAGCCTT CCCCAGCTGC GGGCCCTGCC TGGAGCAGCA GGATGAGGAG
CGCAGCACCA GGCTCAAGGA CCTGCTCGGC CGCTACTTTG AGGAAGAGGA AGGGCAGGAG
CCGGGAGGCA TGGAGGACGC ACAGGGCCCC GAGCCAGGGC AGGCCAGAGT GAGTGCAGTA
AGGCCAGGCA GCTCATCGGG GTTGCAGGTT CCCTGGGCTG CATGGGGCTT GCTCTGTGGA
TGCAGTGCCA CGGGAGCTCA GAGGAAGCCT GATGTGCCTG TCCACACAGC TCCAGGATTG
GGAGGACCAG GTCCGCTGCG ACATCCGCCA GTTCCTGTCC CTGAGGCCAG AGGAGAAGTT
CTCCAGCAGG GCTGTGGCCC GCATCTTCCA CGGCATCGGA AGCCCCTGCT ACCCGGCCCA
GGTGTACGGG CAGGACCGAC GCTTCTGGAG AAAATACCTG CACCTGAGCT TCCATGCCCT
GGTGGGCCTG GCCACGGAAG AGCTCCTGCA GGTGGCCCGC TGACTGCACT GCATTGGGGG
ATGTCGGGTA GAGCTGGGGT TGTCAGAGGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869482.1
CDS47..3919
Translation

Target ORF information:

RefSeq Version XM_017013993.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013993.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGGTGAG
GAACCTGGGG TAAGCCACAG GGGTGTGGAG GGGCTGTCCC CGCGTCCGCT GAGCCCTGCT
CTGCCCCCAG CCATCGAGAC TTTGCTGTGC TACCTGGAGC TGCACCCACA CCACTGGCTG
GAGCTGCTGG CGACCACCTA TACCCATTGC CGTCTGAACT GCCCTGGGGG CCCTGCCCAG
CTCCAGGCCC TGGCCCACAG GTGTCCCCCT TTGGCTGTGT GCTTGGCCCA GCAGCTGCCT
GAGGACCCAG GGCAAGGCAG CAGCTCCGTG GAGTTTGACA TGGTCAAGCT GGTGGACTCC
ATGGGCTGGG AGCTGGCCTC TGTGCGGCGG GCTCTCTGCC AGCTGCAGTG GGACCACGAG
CCCAGGACAG GTGTGCGGCG TGGGACAGGG GTGCTTGTGG AGTTCAGTGA GCTGGCCTTC
CACCTTCGCA GCCCGGGGGA CTTGACCGCT GAGGAGAAGG ACCAGATATG TGACTTCCTC
TATGGCCGTG TGCAGGCCCG GGAGCGCCAG GCCCTGGCCC GTCTGCGCAG AACCTTCCAG
GCCTTTCACA GCGTAGCCTT CCCCAGCTGC GGGCCCTGCC TGGAGCAGCA GGATGAGGAG
CGCAGCACCA GGCTCAAGGA CCTGCTCGGC CGCTACTTTG AGGAAGAGGA AGGGCAGGAG
CCGGGAGGCA TGGAGGACGC ACAGGGCCCC GAGCCAGGGC AGGCCAGAGT GAGTGCAGTA
AGGCCAGGCA GCTCATCGGG GTTGCAGGTT CCCTGGGCTG CATGGGGCTT GCTCTGTGGA
TGCAGTGCCA CGGGAGCTCA GAGGAAGCCT GATGTGCCTG TCCACACAGC TCCAGGATTG
GGAGGACCAG GTCCGCTGCG ACATCCGCCA GTTCCTGTCC CTGAGGCCAG AGGAGAAGTT
CTCCAGCAGG GCTGTGGCCC GCATCTTCCA CGGCATCGGA AGCCCCTGCT ACCCGGCCCA
GGTGTACGGG CAGGACCGAC GCTTCTGGAG AAAATACCTG CACCTGAGCT TCCATGCCCT
GGTGGGCCTG GCCACGGAAG AGCTCCTGCA GGTGGCCCGC TGACTGCACT GCATTGGGGG
ATGTCGGGTA GAGCTGGGGT TGTCAGAGGC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98300
Clone ID Related Accession (Same CDS sequence) XM_017013994.1
Accession Version XM_017013994.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGGGCAAT
TACGTACGGC TCAACATGAA GCAGAAACAC TACGTGCGGG GCCGGGCACT CCGTAGCAGG
CTCCTCCGCA AGCAGGCATG GAAGCAGAAG TGGCGGAAGA AAGGGGAGTG TTTTGGGGGT
GGTGGTGCCA CAGTCACAAC CAAGGAGTCT TGTTTCCTGA ACGAGCAGTT CGATCACTGG
GCAGCCCAGT GTCCCCGGCC AGCAAGTGAG GAAGACACAG ATGCTGTTGG GCCTGAGCCA
CTGGTTCCTT CACCACAACC TGTACCTGAG GTGCCCAGCC TGGACCCCAC CGTGCTGCCA
CTCTACTCCC TGGGGCCCTC AGGGCAGTTG GCAGAGACGC CGGCTGAGGT GTTCCAGGCC
CTGGAGCAGC TGGGGCACCA AGCCTTTCGC CCTGGGCAGG AGCGTGCAGT CATGCGGATC
CTGTCTGGCA TCTCCACGCT GCTGGTGCTG CCTACAGGTG CCGGCAAGTC CCTGTGCTAC
CAGCTCCCAG CGCTGCTCTA CAGCCGGCGC AGCCCCTGCC TCACGTTGGT CGTCTCTCCC
CTGCTGTCAC TCATGGATGA CCAGGTGTCT GGCCTGCCAC CGTGTCTCAA GGCGGCCTGC
ATACACTCGG GCATGACCAG GAAGCAACGG GAATCTGTCC TGCAGAAGAT TCGGGCAGCC
CAGGTACACG TGCTGATGCT GACACCTGAG GCACTGGTGG GGGCGGGAGG CCTCCCTCCA
GCCGCACAGC TGCCTCCAGT TGCTTTTGCC TGCATTGATG AGGCCCACTG CCTCTCCCAG
TGGTCCCACA ACTTCCGGCC CTGCTACCTG CGCGTCTGCA AGGTGCTTCG GGAGCGCATG
GGCGTGCACT GCTTCCTGGG CCTCACAGCC ACAGCCACAC GCCGCACTGC CAGTGACGTG
GCACAGCACC TGGCTGTGGC TGAAGAGCCT GACCTCCACG GGCCAGCCCC AGTTCCCACC
AACCTGCACC TTTCCGTGTC CATGGACAGG GACACAGACC AGGCACTGTT GACGCTGCTG
CAAGGCAAAC GTTTTCAAAA CCTCGATTCC ATTATCATTT ACTGCAACCG GCGCGAGGAC
ACAGAGCGGA TCGCTGCGCT CCTCCGAACC TGCCTGCACG CAGCCTGGGT CCCAGGGTCT
GGAGGTGCGG CATGGACAGA GCTGGTGTCC CCGTGGACCC ACCTTGGGCA CACATGGTCC
CATCCCACTG ACCATCTGCC TGTCTTCCCC AAAGGTCGTG CCCCCAAAAC CACAGCCGAG
GCCTACCACG CGGGCATGTG CAGCCGGGAA CGGCGGCGGG TACAGCGAGC CTTCATGCAG
GGCCAGTTGC GGGTGGTGGT GGCCACGGTG GCCTTTGGGA TGGGGCTGGA CCGGCCAGAT
GTGCGGGCTG TGCTGCATCT GGGGCTGCCC CCAAGCTTCG AGAGCTACGT GCAGGCCGTG
GGCCGGGCCG GGCGTGACGG GCAGCCTGCC CACTGCCACC TCTTCCTGCA GCCCCAGGGC
GAAGACCTGC GAGAGCTGCG CAGACATGTG CACGCCGACA GCACGGACTT CCTGGCTGTG
AAGAGGCTGG TACAGCGCGT GTTCCCAGCC TGCACCTGCA CCTGCACCAG GCCGCCCTCG
GAGCAGGAAG GGGCCGTGGG TGGGGAGAGG CCTGTGCCCA AGTACCCCCC TCAAGAGGCT
GAGCAGCTTA GCCACCAAGC AGCCCCAGGA CCCAGAAGGG TCTGCATGGG CCATGAGCGG
GCACTCCCAA TACAGCTTAC CGTACAGGCT TTGGACATGC CGGAGGAGGG TGAGGAACCT
GGGGTAAGCC ACAGGGGTGT GGAGGGGCTG TCCCCGCGTC CGCTGAGCCC TGCTCTGCCC
CCAGCCATCG AGACTTTGCT GTGCTACCTG GAGCTGCACC CACACCACTG GCTGGAGCTG
CTGGCGACCA CCTATACCCA TTGCCGTCTG AACTGCCCTG GGGGCCCTGC CCAGCTCCAG
GCCCTGGCCC ACAGGTGTCC CCCTTTGGCT GTGTGCTTGG CCCAGCAGCT GCCTGAGGAC
CCAGGGCAAG GCAGCAGCTC CGTGGAGTTT GACATGGTCA AGCTGGTGGA CTCCATGGGC
TGGGAGCTGG CCTCTGTGCG GCGGGCTCTC TGCCAGCTGC AGTGGGACCA CGAGCCCAGG
ACAGGTGTGC GGCGTGGGAC AGGGGTGCTT GTGGAGTTCA GTGAGCTGGC CTTCCACCTT
CGCAGCCCGG GGGACTTGAC CGCTGAGGAG AAGGACCAGA TATGTGACTT CCTCTATGGC
CGTGTGCAGG CCCGGGAGCG CCAGGCCCTG GCCCGTCTGC GCAGAACCTT CCAGGCCTTT
CACAGCGTAG CCTTCCCCAG CTGCGGGCCC TGCCTGGAGC AGCAGGATGA GGAGCGCAGC
ACCAGGCTCA AGGACCTGCT CGGCCGCTAC TTTGAGGAAG AGGAAGGGCA GGAGCCGGGA
GGCATGGAGG ACGCACAGGG CCCCGAGCCA GGGCAGGCCA GAGTGAGTGC AGTAAGGCCA
GGCAGCTCAT CGGGGTTGCA GGTTCCCTGG GCTGCATGGG GCTTGCTCTG TGGATGCAGT
GCCACGGGAG CTCAGAGGAA GCCTGATGTG CCTGTCCACA CAGCTCCAGG ATTGGGAGGA
CCAGGTCCGC TGCGACATCC GCCAGTTCCT GTCCCTGAGG CCAGAGGAGA AGTTCTCCAG
CAGGGCTGTG GCCCGCATCT TCCACGGCAT CGGAAGCCCC TGCTACCCGG CCCAGGTGTA
CGGGCAGGAC CGACGCTTCT GGAGAAAATA CCTGCACCTG AGCTTCCATG CCCTGGTGGG
CCTGGCCACG GAAGAGCTCC TGCAGGTGGC CCGCTGACTG CACTGCATTG GGGGATGTCG
GGTAGAGCTG GGGTTGTCAG AGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869483.1
CDS49..3915
Translation

Target ORF information:

RefSeq Version XM_017013994.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013994.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGGGCAAT
TACGTACGGC TCAACATGAA GCAGAAACAC TACGTGCGGG GCCGGGCACT CCGTAGCAGG
CTCCTCCGCA AGCAGGCATG GAAGCAGAAG TGGCGGAAGA AAGGGGAGTG TTTTGGGGGT
GGTGGTGCCA CAGTCACAAC CAAGGAGTCT TGTTTCCTGA ACGAGCAGTT CGATCACTGG
GCAGCCCAGT GTCCCCGGCC AGCAAGTGAG GAAGACACAG ATGCTGTTGG GCCTGAGCCA
CTGGTTCCTT CACCACAACC TGTACCTGAG GTGCCCAGCC TGGACCCCAC CGTGCTGCCA
CTCTACTCCC TGGGGCCCTC AGGGCAGTTG GCAGAGACGC CGGCTGAGGT GTTCCAGGCC
CTGGAGCAGC TGGGGCACCA AGCCTTTCGC CCTGGGCAGG AGCGTGCAGT CATGCGGATC
CTGTCTGGCA TCTCCACGCT GCTGGTGCTG CCTACAGGTG CCGGCAAGTC CCTGTGCTAC
CAGCTCCCAG CGCTGCTCTA CAGCCGGCGC AGCCCCTGCC TCACGTTGGT CGTCTCTCCC
CTGCTGTCAC TCATGGATGA CCAGGTGTCT GGCCTGCCAC CGTGTCTCAA GGCGGCCTGC
ATACACTCGG GCATGACCAG GAAGCAACGG GAATCTGTCC TGCAGAAGAT TCGGGCAGCC
CAGGTACACG TGCTGATGCT GACACCTGAG GCACTGGTGG GGGCGGGAGG CCTCCCTCCA
GCCGCACAGC TGCCTCCAGT TGCTTTTGCC TGCATTGATG AGGCCCACTG CCTCTCCCAG
TGGTCCCACA ACTTCCGGCC CTGCTACCTG CGCGTCTGCA AGGTGCTTCG GGAGCGCATG
GGCGTGCACT GCTTCCTGGG CCTCACAGCC ACAGCCACAC GCCGCACTGC CAGTGACGTG
GCACAGCACC TGGCTGTGGC TGAAGAGCCT GACCTCCACG GGCCAGCCCC AGTTCCCACC
AACCTGCACC TTTCCGTGTC CATGGACAGG GACACAGACC AGGCACTGTT GACGCTGCTG
CAAGGCAAAC GTTTTCAAAA CCTCGATTCC ATTATCATTT ACTGCAACCG GCGCGAGGAC
ACAGAGCGGA TCGCTGCGCT CCTCCGAACC TGCCTGCACG CAGCCTGGGT CCCAGGGTCT
GGAGGTGCGG CATGGACAGA GCTGGTGTCC CCGTGGACCC ACCTTGGGCA CACATGGTCC
CATCCCACTG ACCATCTGCC TGTCTTCCCC AAAGGTCGTG CCCCCAAAAC CACAGCCGAG
GCCTACCACG CGGGCATGTG CAGCCGGGAA CGGCGGCGGG TACAGCGAGC CTTCATGCAG
GGCCAGTTGC GGGTGGTGGT GGCCACGGTG GCCTTTGGGA TGGGGCTGGA CCGGCCAGAT
GTGCGGGCTG TGCTGCATCT GGGGCTGCCC CCAAGCTTCG AGAGCTACGT GCAGGCCGTG
GGCCGGGCCG GGCGTGACGG GCAGCCTGCC CACTGCCACC TCTTCCTGCA GCCCCAGGGC
GAAGACCTGC GAGAGCTGCG CAGACATGTG CACGCCGACA GCACGGACTT CCTGGCTGTG
AAGAGGCTGG TACAGCGCGT GTTCCCAGCC TGCACCTGCA CCTGCACCAG GCCGCCCTCG
GAGCAGGAAG GGGCCGTGGG TGGGGAGAGG CCTGTGCCCA AGTACCCCCC TCAAGAGGCT
GAGCAGCTTA GCCACCAAGC AGCCCCAGGA CCCAGAAGGG TCTGCATGGG CCATGAGCGG
GCACTCCCAA TACAGCTTAC CGTACAGGCT TTGGACATGC CGGAGGAGGG TGAGGAACCT
GGGGTAAGCC ACAGGGGTGT GGAGGGGCTG TCCCCGCGTC CGCTGAGCCC TGCTCTGCCC
CCAGCCATCG AGACTTTGCT GTGCTACCTG GAGCTGCACC CACACCACTG GCTGGAGCTG
CTGGCGACCA CCTATACCCA TTGCCGTCTG AACTGCCCTG GGGGCCCTGC CCAGCTCCAG
GCCCTGGCCC ACAGGTGTCC CCCTTTGGCT GTGTGCTTGG CCCAGCAGCT GCCTGAGGAC
CCAGGGCAAG GCAGCAGCTC CGTGGAGTTT GACATGGTCA AGCTGGTGGA CTCCATGGGC
TGGGAGCTGG CCTCTGTGCG GCGGGCTCTC TGCCAGCTGC AGTGGGACCA CGAGCCCAGG
ACAGGTGTGC GGCGTGGGAC AGGGGTGCTT GTGGAGTTCA GTGAGCTGGC CTTCCACCTT
CGCAGCCCGG GGGACTTGAC CGCTGAGGAG AAGGACCAGA TATGTGACTT CCTCTATGGC
CGTGTGCAGG CCCGGGAGCG CCAGGCCCTG GCCCGTCTGC GCAGAACCTT CCAGGCCTTT
CACAGCGTAG CCTTCCCCAG CTGCGGGCCC TGCCTGGAGC AGCAGGATGA GGAGCGCAGC
ACCAGGCTCA AGGACCTGCT CGGCCGCTAC TTTGAGGAAG AGGAAGGGCA GGAGCCGGGA
GGCATGGAGG ACGCACAGGG CCCCGAGCCA GGGCAGGCCA GAGTGAGTGC AGTAAGGCCA
GGCAGCTCAT CGGGGTTGCA GGTTCCCTGG GCTGCATGGG GCTTGCTCTG TGGATGCAGT
GCCACGGGAG CTCAGAGGAA GCCTGATGTG CCTGTCCACA CAGCTCCAGG ATTGGGAGGA
CCAGGTCCGC TGCGACATCC GCCAGTTCCT GTCCCTGAGG CCAGAGGAGA AGTTCTCCAG
CAGGGCTGTG GCCCGCATCT TCCACGGCAT CGGAAGCCCC TGCTACCCGG CCCAGGTGTA
CGGGCAGGAC CGACGCTTCT GGAGAAAATA CCTGCACCTG AGCTTCCATG CCCTGGTGGG
CCTGGCCACG GAAGAGCTCC TGCAGGTGGC CCGCTGACTG CACTGCATTG GGGGATGTCG
GGTAGAGCTG GGGTTGTCAG AGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98301
Clone ID Related Accession (Same CDS sequence) XM_017013995.1
Accession Version XM_017013995.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGCCATC
GAGACTTTGC TGTGCTACCT GGAGCTGCAC CCACACCACT GGCTGGAGCT GCTGGCGACC
ACCTATACCC ATTGCCGTCT GAACTGCCCT GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC
CACAGGTGTC CCCCTTTGGC TGTGTGCTTG GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA
GGCAGCAGCT CCGTGGAGTT TGACATGGTC AAGCTGGTGG ACTCCATGGG CTGGGAGCTG
GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG CAGTGGGACC ACGAGCCCAG GACAGGTGTG
CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC AGTGAGCTGG CCTTCCACCT TCGCAGCCCG
GGGGACTTGA CCGCTGAGGA GAAGGACCAG ATATGTGACT TCCTCTATGG CCGTGTGCAG
GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG CGCAGAACCT TCCAGGCCTT TCACAGCGTA
GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG CAGCAGGATG AGGAGCGCAG CACCAGGCTC
AAGGACCTGC TCGGCCGCTA CTTTGAGGAA GAGGAAGGGC AGGAGCCGGG AGGCATGGAG
GACGCACAGG GCCCCGAGCC AGGGCAGGCC AGAGTGAGTG CAGTAAGGCC AGGCAGCTCA
TCGGGGTTGC AGGTTCCCTG GGCTGCATGG GGCTTGCTCT GTGGATGCAG TGCCACGGGA
GCTCAGAGGA AGCCTGATGT GCCTGTCCAC ACAGCTCCAG GATTGGGAGG ACCAGGTCCG
CTGCGACATC CGCCAGTTCC TGTCCCTGAG GCCAGAGGAG AAGTTCTCCA GCAGGGCTGT
GGCCCGCATC TTCCACGGCA TCGGAAGCCC CTGCTACCCG GCCCAGGTGT ACGGGCAGGA
CCGACGCTTC TGGAGAAAAT ACCTGCACCT GAGCTTCCAT GCCCTGGTGG GCCTGGCCAC
GGAAGAGCTC CTGCAGGTGG CCCGCTGACT GCACTGCATT GGGGGATGTC GGGTAGAGCT
GGGGTTGTCA GAGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869484.1
CDS45..3842
Translation

Target ORF information:

RefSeq Version XM_017013995.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013995.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGCCATC
GAGACTTTGC TGTGCTACCT GGAGCTGCAC CCACACCACT GGCTGGAGCT GCTGGCGACC
ACCTATACCC ATTGCCGTCT GAACTGCCCT GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC
CACAGGTGTC CCCCTTTGGC TGTGTGCTTG GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA
GGCAGCAGCT CCGTGGAGTT TGACATGGTC AAGCTGGTGG ACTCCATGGG CTGGGAGCTG
GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG CAGTGGGACC ACGAGCCCAG GACAGGTGTG
CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC AGTGAGCTGG CCTTCCACCT TCGCAGCCCG
GGGGACTTGA CCGCTGAGGA GAAGGACCAG ATATGTGACT TCCTCTATGG CCGTGTGCAG
GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG CGCAGAACCT TCCAGGCCTT TCACAGCGTA
GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG CAGCAGGATG AGGAGCGCAG CACCAGGCTC
AAGGACCTGC TCGGCCGCTA CTTTGAGGAA GAGGAAGGGC AGGAGCCGGG AGGCATGGAG
GACGCACAGG GCCCCGAGCC AGGGCAGGCC AGAGTGAGTG CAGTAAGGCC AGGCAGCTCA
TCGGGGTTGC AGGTTCCCTG GGCTGCATGG GGCTTGCTCT GTGGATGCAG TGCCACGGGA
GCTCAGAGGA AGCCTGATGT GCCTGTCCAC ACAGCTCCAG GATTGGGAGG ACCAGGTCCG
CTGCGACATC CGCCAGTTCC TGTCCCTGAG GCCAGAGGAG AAGTTCTCCA GCAGGGCTGT
GGCCCGCATC TTCCACGGCA TCGGAAGCCC CTGCTACCCG GCCCAGGTGT ACGGGCAGGA
CCGACGCTTC TGGAGAAAAT ACCTGCACCT GAGCTTCCAT GCCCTGGTGG GCCTGGCCAC
GGAAGAGCTC CTGCAGGTGG CCCGCTGACT GCACTGCATT GGGGGATGTC GGGTAGAGCT
GGGGTTGTCA GAGGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98302
Clone ID Related Accession (Same CDS sequence) XM_017013996.1
Accession Version XM_017013996.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGTACA GCGAGCCTTC ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC
ACGGTGGCCT TTGGGATGGG GCTGGACCGG CCAGATGTGC GGGCTGTGCT GCATCTGGGG
CTGCCCCCAA GCTTCGAGAG CTACGTGCAG GCCGTGGGCC GGGCCGGGCG TGACGGGCAG
CCTGCCCACT GCCACCTCTT CCTGCAGCCC CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA
CATGTGCACG CCGACAGCAC GGACTTCCTG GCTGTGAAGA GGCTGGTACA GCGCGTGTTC
CCAGCCTGCA CCTGCACCTG CACCAGGCCG CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG
GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC
CCAGGACCCA GAAGGGTCTG CATGGGCCAT GAGCGGGCAC TCCCAATACA GCTTACCGTA
CAGGCTTTGG ACATGCCGGA GGAGGGTGAG GAACCTGGGG TAAGCCACAG GGGTGTGGAG
GGGCTGTCCC CGCGTCCGCT GAGCCCTGCT CTGCCCCCAG CCATCGAGAC TTTGCTGTGC
TACCTGGAGC TGCACCCACA CCACTGGCTG GAGCTGCTGG CGACCACCTA TACCCATTGC
CGTCTGAACT GCCCTGGGGG CCCTGCCCAG CTCCAGGCCC TGGCCCACAG GTGTCCCCCT
TTGGCTGTGT GCTTGGCCCA GCAGCTGCCT GAGGACCCAG GGCAAGGCAG CAGCTCCGTG
GAGTTTGACA TGGTCAAGCT GGTGGACTCC ATGGGCTGGG AGCTGGCCTC TGTGCGGCGG
GCTCTCTGCC AGCTGCAGTG GGACCACGAG CCCAGGACAG GTGTGCGGCG TGGGACAGGG
GTGCTTGTGG AGTTCAGTGA GCTGGCCTTC CACCTTCGCA GCCCGGGGGA CTTGACCGCT
GAGGAGAAGG ACCAGATATG TGACTTCCTC TATGGCCGTG TGCAGGCCCG GGAGCGCCAG
GCCCTGGCCC GTCTGCGCAG AACCTTCCAG GCCTTTCACA GCGTAGCCTT CCCCAGCTGC
GGGCCCTGCC TGGAGCAGCA GGATGAGGAG CGCAGCACCA GGCTCAAGGA CCTGCTCGGC
CGCTACTTTG AGGAAGAGGA AGGGCAGGAG CCGGGAGGCA TGGAGGACGC ACAGGGCCCC
GAGCCAGGGC AGGCCAGACT CCAGGATTGG GAGGACCAGG TCCGCTGCGA CATCCGCCAG
TTCCTGTCCC TGAGGCCAGA GGAGAAGTTC TCCAGCAGGG CTGTGGCCCG CATCTTCCAC
GGCATCGGAA GCCCCTGCTA CCCGGCCCAG GTGTACGGGC AGGACCGACG CTTCTGGAGA
AAATACCTGC ACCTGAGCTT CCATGCCCTG GTGGGCCTGG CCACGGAAGA GCTCCTGCAG
GTGGCCCGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869485.1
CDS46..3837
Translation

Target ORF information:

RefSeq Version XM_017013996.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013996.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGTACA GCGAGCCTTC ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC
ACGGTGGCCT TTGGGATGGG GCTGGACCGG CCAGATGTGC GGGCTGTGCT GCATCTGGGG
CTGCCCCCAA GCTTCGAGAG CTACGTGCAG GCCGTGGGCC GGGCCGGGCG TGACGGGCAG
CCTGCCCACT GCCACCTCTT CCTGCAGCCC CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA
CATGTGCACG CCGACAGCAC GGACTTCCTG GCTGTGAAGA GGCTGGTACA GCGCGTGTTC
CCAGCCTGCA CCTGCACCTG CACCAGGCCG CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG
GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC
CCAGGACCCA GAAGGGTCTG CATGGGCCAT GAGCGGGCAC TCCCAATACA GCTTACCGTA
CAGGCTTTGG ACATGCCGGA GGAGGGTGAG GAACCTGGGG TAAGCCACAG GGGTGTGGAG
GGGCTGTCCC CGCGTCCGCT GAGCCCTGCT CTGCCCCCAG CCATCGAGAC TTTGCTGTGC
TACCTGGAGC TGCACCCACA CCACTGGCTG GAGCTGCTGG CGACCACCTA TACCCATTGC
CGTCTGAACT GCCCTGGGGG CCCTGCCCAG CTCCAGGCCC TGGCCCACAG GTGTCCCCCT
TTGGCTGTGT GCTTGGCCCA GCAGCTGCCT GAGGACCCAG GGCAAGGCAG CAGCTCCGTG
GAGTTTGACA TGGTCAAGCT GGTGGACTCC ATGGGCTGGG AGCTGGCCTC TGTGCGGCGG
GCTCTCTGCC AGCTGCAGTG GGACCACGAG CCCAGGACAG GTGTGCGGCG TGGGACAGGG
GTGCTTGTGG AGTTCAGTGA GCTGGCCTTC CACCTTCGCA GCCCGGGGGA CTTGACCGCT
GAGGAGAAGG ACCAGATATG TGACTTCCTC TATGGCCGTG TGCAGGCCCG GGAGCGCCAG
GCCCTGGCCC GTCTGCGCAG AACCTTCCAG GCCTTTCACA GCGTAGCCTT CCCCAGCTGC
GGGCCCTGCC TGGAGCAGCA GGATGAGGAG CGCAGCACCA GGCTCAAGGA CCTGCTCGGC
CGCTACTTTG AGGAAGAGGA AGGGCAGGAG CCGGGAGGCA TGGAGGACGC ACAGGGCCCC
GAGCCAGGGC AGGCCAGACT CCAGGATTGG GAGGACCAGG TCCGCTGCGA CATCCGCCAG
TTCCTGTCCC TGAGGCCAGA GGAGAAGTTC TCCAGCAGGG CTGTGGCCCG CATCTTCCAC
GGCATCGGAA GCCCCTGCTA CCCGGCCCAG GTGTACGGGC AGGACCGACG CTTCTGGAGA
AAATACCTGC ACCTGAGCTT CCATGCCCTG GTGGGCCTGG CCACGGAAGA GCTCCTGCAG
GTGGCCCGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98303
Clone ID Related Accession (Same CDS sequence) XM_017013997.1
Accession Version XM_017013997.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3765bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGGCGA AGACCTGCGA GAGCTGCGCA GACATGTGCA CGCCGACAGC
ACGGACTTCC TGGCTGTGAA GAGGCTGGTA CAGCGCGTGT TCCCAGCCTG CACCTGCACC
TGCACCAGGC CGCCCTCGGA GCAGGAAGGG GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG
TACCCCCCTC AAGAGGCTGA GCAGCTTAGC CACCAAGCAG CCCCAGGACC CAGAAGGGTC
TGCATGGGCC ATGAGCGGGC ACTCCCAATA CAGCTTACCG TACAGGCTTT GGACATGCCG
GAGGAGGGTG AGGAACCTGG GGTAAGCCAC AGGGGTGTGG AGGGGCTGTC CCCGCGTCCG
CTGAGCCCTG CTCTGCCCCC AGCCATCGAG ACTTTGCTGT GCTACCTGGA GCTGCACCCA
CACCACTGGC TGGAGCTGCT GGCGACCACC TATACCCATT GCCGTCTGAA CTGCCCTGGG
GGCCCTGCCC AGCTCCAGGC CCTGGCCCAC AGGTGTCCCC CTTTGGCTGT GTGCTTGGCC
CAGCAGCTGC CTGAGGACCC AGGGCAAGGC AGCAGCTCCG TGGAGTTTGA CATGGTCAAG
CTGGTGGACT CCATGGGCTG GGAGCTGGCC TCTGTGCGGC GGGCTCTCTG CCAGCTGCAG
TGGGACCACG AGCCCAGGAC AGGTGTGCGG CGTGGGACAG GGGTGCTTGT GGAGTTCAGT
GAGCTGGCCT TCCACCTTCG CAGCCCGGGG GACTTGACCG CTGAGGAGAA GGACCAGATA
TGTGACTTCC TCTATGGCCG TGTGCAGGCC CGGGAGCGCC AGGCCCTGGC CCGTCTGCGC
AGAACCTTCC AGGCCTTTCA CAGCGTAGCC TTCCCCAGCT GCGGGCCCTG CCTGGAGCAG
CAGGATGAGG AGCGCAGCAC CAGGCTCAAG GACCTGCTCG GCCGCTACTT TGAGGAAGAG
GAAGGGCAGG AGCCGGGAGG CATGGAGGAC GCACAGGGCC CCGAGCCAGG GCAGGCCAGA
GTGAGTGCAG TAAGGCCAGG CAGCTCATCG GGGTTGCAGG TTCCCTGGGC TGCATGGGGC
TTGCTCTGTG GATGCAGTGC CACGGGAGCT CAGAGGAAGC CTGATGTGCC TGTCCACACA
GCTCCAGGAT TGGGAGGACC AGGTCCGCTG CGACATCCGC CAGTTCCTGT CCCTGAGGCC
AGAGGAGAAG TTCTCCAGCA GGGCTGTGGC CCGCATCTTC CACGGCATCG GAAGCCCCTG
CTACCCGGCC CAGGTGTACG GGCAGGACCG ACGCTTCTGG AGAAAATACC TGCACCTGAG
CTTCCATGCC CTGGTGGGCC TGGCCACGGA AGAGCTCCTG CAGGTGGCCC GCTGACTGCA
CTGCATTGGG GGATGTCGGG TAGAGCTGGG GTTGTCAGAG GCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869486.1
CDS48..3812
Translation

Target ORF information:

RefSeq Version XM_017013997.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013997.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGGCGA AGACCTGCGA GAGCTGCGCA GACATGTGCA CGCCGACAGC
ACGGACTTCC TGGCTGTGAA GAGGCTGGTA CAGCGCGTGT TCCCAGCCTG CACCTGCACC
TGCACCAGGC CGCCCTCGGA GCAGGAAGGG GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG
TACCCCCCTC AAGAGGCTGA GCAGCTTAGC CACCAAGCAG CCCCAGGACC CAGAAGGGTC
TGCATGGGCC ATGAGCGGGC ACTCCCAATA CAGCTTACCG TACAGGCTTT GGACATGCCG
GAGGAGGGTG AGGAACCTGG GGTAAGCCAC AGGGGTGTGG AGGGGCTGTC CCCGCGTCCG
CTGAGCCCTG CTCTGCCCCC AGCCATCGAG ACTTTGCTGT GCTACCTGGA GCTGCACCCA
CACCACTGGC TGGAGCTGCT GGCGACCACC TATACCCATT GCCGTCTGAA CTGCCCTGGG
GGCCCTGCCC AGCTCCAGGC CCTGGCCCAC AGGTGTCCCC CTTTGGCTGT GTGCTTGGCC
CAGCAGCTGC CTGAGGACCC AGGGCAAGGC AGCAGCTCCG TGGAGTTTGA CATGGTCAAG
CTGGTGGACT CCATGGGCTG GGAGCTGGCC TCTGTGCGGC GGGCTCTCTG CCAGCTGCAG
TGGGACCACG AGCCCAGGAC AGGTGTGCGG CGTGGGACAG GGGTGCTTGT GGAGTTCAGT
GAGCTGGCCT TCCACCTTCG CAGCCCGGGG GACTTGACCG CTGAGGAGAA GGACCAGATA
TGTGACTTCC TCTATGGCCG TGTGCAGGCC CGGGAGCGCC AGGCCCTGGC CCGTCTGCGC
AGAACCTTCC AGGCCTTTCA CAGCGTAGCC TTCCCCAGCT GCGGGCCCTG CCTGGAGCAG
CAGGATGAGG AGCGCAGCAC CAGGCTCAAG GACCTGCTCG GCCGCTACTT TGAGGAAGAG
GAAGGGCAGG AGCCGGGAGG CATGGAGGAC GCACAGGGCC CCGAGCCAGG GCAGGCCAGA
GTGAGTGCAG TAAGGCCAGG CAGCTCATCG GGGTTGCAGG TTCCCTGGGC TGCATGGGGC
TTGCTCTGTG GATGCAGTGC CACGGGAGCT CAGAGGAAGC CTGATGTGCC TGTCCACACA
GCTCCAGGAT TGGGAGGACC AGGTCCGCTG CGACATCCGC CAGTTCCTGT CCCTGAGGCC
AGAGGAGAAG TTCTCCAGCA GGGCTGTGGC CCGCATCTTC CACGGCATCG GAAGCCCCTG
CTACCCGGCC CAGGTGTACG GGCAGGACCG ACGCTTCTGG AGAAAATACC TGCACCTGAG
CTTCCATGCC CTGGTGGGCC TGGCCACGGA AGAGCTCCTG CAGGTGGCCC GCTGACTGCA
CTGCATTGGG GGATGTCGGG TAGAGCTGGG GTTGTCAGAG GCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98304
Clone ID Related Accession (Same CDS sequence) XM_017013998.1
Accession Version XM_017013998.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3717bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X8
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGTACA GCGAGCCTTC ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC
ACGGTGGCCT TTGGGATGGG GCTGGACCGG CCAGATGTGC GGGCTGTGCT GCATCTGGGG
CTGCCCCCAA GCTTCGAGAG CTACGTGCAG GCCGTGGGCC GGGCCGGGCG TGACGGGCAG
CCTGCCCACT GCCACCTCTT CCTGCAGCCC CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA
CATGTGCACG CCGACAGCAC GGACTTCCTG GCTGTGAAGA GGCTGGTACA GCGCGTGTTC
CCAGCCTGCA CCTGCACCTG CACCAGGCCG CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG
GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC
CCAGGACCCA GAAGGGTCTG CATGGGCCAT GAGCGGGCAC TCCCAATACA GCTTACCGTA
CAGGCTTTGG ACATGCCGGA GGAGGCCATC GAGACTTTGC TGTGCTACCT GGAGCTGCAC
CCACACCACT GGCTGGAGCT GCTGGCGACC ACCTATACCC ATTGCCGTCT GAACTGCCCT
GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC CACAGGTGTC CCCCTTTGGC TGTGTGCTTG
GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA GGCAGCAGCT CCGTGGAGTT TGACATGGTC
AAGCTGGTGG ACTCCATGGG CTGGGAGCTG GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG
CAGTGGGACC ACGAGCCCAG GACAGGTGTG CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC
AGTGAGCTGG CCTTCCACCT TCGCAGCCCG GGGGACTTGA CCGCTGAGGA GAAGGACCAG
ATATGTGACT TCCTCTATGG CCGTGTGCAG GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG
CGCAGAACCT TCCAGGCCTT TCACAGCGTA GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG
CAGCAGGATG AGGAGCGCAG CACCAGGCTC AAGGACCTGC TCGGCCGCTA CTTTGAGGAA
GAGGAAGGGC AGGAGCCGGG AGGCATGGAG GACGCACAGG GCCCCGAGCC AGGGCAGGCC
AGACTCCAGG ATTGGGAGGA CCAGGTCCGC TGCGACATCC GCCAGTTCCT GTCCCTGAGG
CCAGAGGAGA AGTTCTCCAG CAGGGCTGTG GCCCGCATCT TCCACGGCAT CGGAAGCCCC
TGCTACCCGG CCCAGGTGTA CGGGCAGGAC CGACGCTTCT GGAGAAAATA CCTGCACCTG
AGCTTCCATG CCCTGGTGGG CCTGGCCACG GAAGAGCTCC TGCAGGTGGC CCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869487.1
CDS45..3761
Translation

Target ORF information:

RefSeq Version XM_017013998.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013998.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTGCGGCATG GACAGAGCTG
GTGTCCCCGT GGACCCACCT TGGGCACACA TGGTCCCATC CCACTGACCA TCTGCCTGTC
TTCCCCAAAG GTCGTGCCCC CAAAACCACA GCCGAGGCCT ACCACGCGGG CATGTGCAGC
CGGGAACGGC GGCGGGTACA GCGAGCCTTC ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC
ACGGTGGCCT TTGGGATGGG GCTGGACCGG CCAGATGTGC GGGCTGTGCT GCATCTGGGG
CTGCCCCCAA GCTTCGAGAG CTACGTGCAG GCCGTGGGCC GGGCCGGGCG TGACGGGCAG
CCTGCCCACT GCCACCTCTT CCTGCAGCCC CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA
CATGTGCACG CCGACAGCAC GGACTTCCTG GCTGTGAAGA GGCTGGTACA GCGCGTGTTC
CCAGCCTGCA CCTGCACCTG CACCAGGCCG CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG
GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC
CCAGGACCCA GAAGGGTCTG CATGGGCCAT GAGCGGGCAC TCCCAATACA GCTTACCGTA
CAGGCTTTGG ACATGCCGGA GGAGGCCATC GAGACTTTGC TGTGCTACCT GGAGCTGCAC
CCACACCACT GGCTGGAGCT GCTGGCGACC ACCTATACCC ATTGCCGTCT GAACTGCCCT
GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC CACAGGTGTC CCCCTTTGGC TGTGTGCTTG
GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA GGCAGCAGCT CCGTGGAGTT TGACATGGTC
AAGCTGGTGG ACTCCATGGG CTGGGAGCTG GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG
CAGTGGGACC ACGAGCCCAG GACAGGTGTG CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC
AGTGAGCTGG CCTTCCACCT TCGCAGCCCG GGGGACTTGA CCGCTGAGGA GAAGGACCAG
ATATGTGACT TCCTCTATGG CCGTGTGCAG GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG
CGCAGAACCT TCCAGGCCTT TCACAGCGTA GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG
CAGCAGGATG AGGAGCGCAG CACCAGGCTC AAGGACCTGC TCGGCCGCTA CTTTGAGGAA
GAGGAAGGGC AGGAGCCGGG AGGCATGGAG GACGCACAGG GCCCCGAGCC AGGGCAGGCC
AGACTCCAGG ATTGGGAGGA CCAGGTCCGC TGCGACATCC GCCAGTTCCT GTCCCTGAGG
CCAGAGGAGA AGTTCTCCAG CAGGGCTGTG GCCCGCATCT TCCACGGCAT CGGAAGCCCC
TGCTACCCGG CCCAGGTGTA CGGGCAGGAC CGACGCTTCT GGAGAAAATA CCTGCACCTG
AGCTTCCATG CCCTGGTGGG CCTGGCCACG GAAGAGCTCC TGCAGGTGGC CCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu98305
Clone ID Related Accession (Same CDS sequence) XM_017013999.1
Accession Version XM_017013999.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X9
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGGCGA AGACCTGCGA
GAGCTGCGCA GACATGTGCA CGCCGACAGC ACGGACTTCC TGGCTGTGAA GAGGCTGGTA
CAGCGCGTGT TCCCAGCCTG CACCTGCACC TGCACCAGGC CGCCCTCGGA GCAGGAAGGG
GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG TACCCCCCTC AAGAGGCTGA GCAGCTTAGC
CACCAAGCAG CCCCAGGACC CAGAAGGGTC TGCATGGGCC ATGAGCGGGC ACTCCCAATA
CAGCTTACCG TACAGGCTTT GGACATGCCG GAGGAGGGTG AGGAACCTGG GGTAAGCCAC
AGGGGTGTGG AGGGGCTGTC CCCGCGTCCG CTGAGCCCTG CTCTGCCCCC AGCCATCGAG
ACTTTGCTGT GCTACCTGGA GCTGCACCCA CACCACTGGC TGGAGCTGCT GGCGACCACC
TATACCCATT GCCGTCTGAA CTGCCCTGGG GGCCCTGCCC AGCTCCAGGC CCTGGCCCAC
AGGTGTCCCC CTTTGGCTGT GTGCTTGGCC CAGCAGCTGC CTGAGGACCC AGGGCAAGGC
AGCAGCTCCG TGGAGTTTGA CATGGTCAAG CTGGTGGACT CCATGGGCTG GGAGCTGGCC
TCTGTGCGGC GGGCTCTCTG CCAGCTGCAG TGGGACCACG AGCCCAGGAC AGGTGTGCGG
CGTGGGACAG GGGTGCTTGT GGAGTTCAGT GAGCTGGCCT TCCACCTTCG CAGCCCGGGG
GACTTGACCG CTGAGGAGAA GGACCAGATA TGTGACTTCC TCTATGGCCG TGTGCAGGCC
CGGGAGCGCC AGGCCCTGGC CCGTCTGCGC AGAACCTTCC AGGCCTTTCA CAGCGTAGCC
TTCCCCAGCT GCGGGCCCTG CCTGGAGCAG CAGGATGAGG AGCGCAGCAC CAGGCTCAAG
GACCTGCTCG GCCGCTACTT TGAGGAAGAG GAAGGGCAGG AGCCGGGAGG CATGGAGGAC
GCACAGGGCC CCGAGCCAGG GCAGGCCAGA GTGAGTGCAG TAAGGCCAGG CAGCTCATCG
GGGTTGCAGG TTCCCTGGGC TGCATGGGGC TTGCTCTGTG GATGCAGTGC CACGGGAGCT
CAGAGGAAGC CTGATGTGCC TGTCCACACA GCTCCAGGAT TGGGAGGACC AGGTCCGCTG
CGACATCCGC CAGTTCCTGT CCCTGAGGCC AGAGGAGAAG TTCTCCAGCA GGGCTGTGGC
CCGCATCTTC CACGGCATCG GAAGCCCCTG CTACCCGGCC CAGGTGTACG GGCAGGACCG
ACGCTTCTGG AGAAAATACC TGCACCTGAG CTTCCATGCC CTGGTGGGCC TGGCCACGGA
AGAGCTCCTG CAGGTGGCCC GCTGACTGCA CTGCATTGGG GGATGTCGGG TAGAGCTGGG
GTTGTCAGAG GCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016869488.1
CDS47..3721
Translation

Target ORF information:

RefSeq Version XM_017013999.1
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017013999.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGGCGA AGACCTGCGA
GAGCTGCGCA GACATGTGCA CGCCGACAGC ACGGACTTCC TGGCTGTGAA GAGGCTGGTA
CAGCGCGTGT TCCCAGCCTG CACCTGCACC TGCACCAGGC CGCCCTCGGA GCAGGAAGGG
GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG TACCCCCCTC AAGAGGCTGA GCAGCTTAGC
CACCAAGCAG CCCCAGGACC CAGAAGGGTC TGCATGGGCC ATGAGCGGGC ACTCCCAATA
CAGCTTACCG TACAGGCTTT GGACATGCCG GAGGAGGGTG AGGAACCTGG GGTAAGCCAC
AGGGGTGTGG AGGGGCTGTC CCCGCGTCCG CTGAGCCCTG CTCTGCCCCC AGCCATCGAG
ACTTTGCTGT GCTACCTGGA GCTGCACCCA CACCACTGGC TGGAGCTGCT GGCGACCACC
TATACCCATT GCCGTCTGAA CTGCCCTGGG GGCCCTGCCC AGCTCCAGGC CCTGGCCCAC
AGGTGTCCCC CTTTGGCTGT GTGCTTGGCC CAGCAGCTGC CTGAGGACCC AGGGCAAGGC
AGCAGCTCCG TGGAGTTTGA CATGGTCAAG CTGGTGGACT CCATGGGCTG GGAGCTGGCC
TCTGTGCGGC GGGCTCTCTG CCAGCTGCAG TGGGACCACG AGCCCAGGAC AGGTGTGCGG
CGTGGGACAG GGGTGCTTGT GGAGTTCAGT GAGCTGGCCT TCCACCTTCG CAGCCCGGGG
GACTTGACCG CTGAGGAGAA GGACCAGATA TGTGACTTCC TCTATGGCCG TGTGCAGGCC
CGGGAGCGCC AGGCCCTGGC CCGTCTGCGC AGAACCTTCC AGGCCTTTCA CAGCGTAGCC
TTCCCCAGCT GCGGGCCCTG CCTGGAGCAG CAGGATGAGG AGCGCAGCAC CAGGCTCAAG
GACCTGCTCG GCCGCTACTT TGAGGAAGAG GAAGGGCAGG AGCCGGGAGG CATGGAGGAC
GCACAGGGCC CCGAGCCAGG GCAGGCCAGA GTGAGTGCAG TAAGGCCAGG CAGCTCATCG
GGGTTGCAGG TTCCCTGGGC TGCATGGGGC TTGCTCTGTG GATGCAGTGC CACGGGAGCT
CAGAGGAAGC CTGATGTGCC TGTCCACACA GCTCCAGGAT TGGGAGGACC AGGTCCGCTG
CGACATCCGC CAGTTCCTGT CCCTGAGGCC AGAGGAGAAG TTCTCCAGCA GGGCTGTGGC
CCGCATCTTC CACGGCATCG GAAGCCCCTG CTACCCGGCC CAGGTGTACG GGCAGGACCG
ACGCTTCTGG AGAAAATACC TGCACCTGAG CTTCCATGCC CTGGTGGGCC TGGCCACGGA
AGAGCTCCTG CAGGTGGCCC GCTGACTGCA CTGCATTGGG GGATGTCGGG TAGAGCTGGG
GTTGTCAGAG GCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18758
Clone ID Related Accession (Same CDS sequence) NM_004260.3
Accession Version NM_004260.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-08
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN286057.1, AB006532.1 and BC013277.2. This sequence is a reference standard in the RefSeqGene project. On Jan 16, 2010 this sequence version replaced NM_004260.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB006532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGCCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGCCATC
GAGACTTTGC TGTGCTACCT GGAGCTGCAC CCACACCACT GGCTGGAGCT GCTGGCGACC
ACCTATACCC ATTGCCGTCT GAACTGCCCT GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC
CACAGGTGTC CCCCTTTGGC TGTGTGCTTG GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA
GGCAGCAGCT CCGTGGAGTT TGACATGGTC AAGCTGGTGG ACTCCATGGG CTGGGAGCTG
GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG CAGTGGGACC ACGAGCCCAG GACAGGTGTG
CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC AGTGAGCTGG CCTTCCACCT TCGCAGCCCG
GGGGACCTGA CCGCTGAGGA GAAGGACCAG ATATGTGACT TCCTCTATGG CCGTGTGCAG
GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG CGCAGAACCT TCCAGGCCTT TCACAGCGTA
GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG CAGCAGGATG AGGAGCGCAG CACCAGGCTC
AAGGACCTGC TCGGCCGCTA CTTTGAGGAA GAGGAAGGGC AGGAGCCGGG AGGCATGGAG
GACGCACAGG GCCCCGAGCC AGGGCAGGCC AGACTCCAGG ATTGGGAGGA CCAGGTCCGC
TGCGACATCC GCCAGTTCCT GTCCCTGAGG CCAGAGGAGA AGTTCTCCAG CAGGGCTGTG
GCCCGCATCT TCCACGGCAT CGGAAGCCCC TGCTACCCGG CCCAGGTGTA CGGGCAGGAC
CGACGCTTCT GGAGAAAATA CCTGCACCTG AGCTTCCATG CCCTGGTGGG CCTGGCCACG
GAAGAGCTCC TGCAGGTGGC CCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004251.3
CDS43..3669
Misc Feature(1)121..123
Misc Feature(2)574..576
Misc Feature(3)580..582
Misc Feature(4)1459..2010
Misc Feature(5)1486..1980
Misc Feature(6)1555..1569
Misc Feature(7)1855..1866
Misc Feature(8)1855..1866
Misc Feature(9)2095..2493
Misc Feature(10)order(2164..2175,2275..2280,2353..2361)
Misc Feature(11)order(2377..2379,2440..2442,2452..2454,2461..2463)
Exon (1)1..126
Exon (2)127..160
Exon (3)161..255
Exon (4)256..396
Exon (5)397..1173
Exon (6)1174..1300
Exon (7)1301..1432
Exon (8)1433..1525
Exon (9)1526..1662
Exon (10)1663..1746
Exon (11)1747..1920
Exon (12)1921..2100
Exon (13)2101..2242
Exon (14)2243..2505
Exon (15)2506..2797
Exon (16)2798..2927
Exon (17)2928..3097
Exon (18)3098..3278
Exon (19)3279..3435
Exon (20)3436..3544
Exon (21)3545..3816
Translation

Target ORF information:

RefSeq Version NM_004260.3
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004260.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGCCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGTACA GCGAGCCTTC
ATGCAGGGCC AGTTGCGGGT GGTGGTGGCC ACGGTGGCCT TTGGGATGGG GCTGGACCGG
CCAGATGTGC GGGCTGTGCT GCATCTGGGG CTGCCCCCAA GCTTCGAGAG CTACGTGCAG
GCCGTGGGCC GGGCCGGGCG TGACGGGCAG CCTGCCCACT GCCACCTCTT CCTGCAGCCC
CAGGGCGAAG ACCTGCGAGA GCTGCGCAGA CATGTGCACG CCGACAGCAC GGACTTCCTG
GCTGTGAAGA GGCTGGTACA GCGCGTGTTC CCAGCCTGCA CCTGCACCTG CACCAGGCCG
CCCTCGGAGC AGGAAGGGGC CGTGGGTGGG GAGAGGCCTG TGCCCAAGTA CCCCCCTCAA
GAGGCTGAGC AGCTTAGCCA CCAAGCAGCC CCAGGACCCA GAAGGGTCTG CATGGGCCAT
GAGCGGGCAC TCCCAATACA GCTTACCGTA CAGGCTTTGG ACATGCCGGA GGAGGCCATC
GAGACTTTGC TGTGCTACCT GGAGCTGCAC CCACACCACT GGCTGGAGCT GCTGGCGACC
ACCTATACCC ATTGCCGTCT GAACTGCCCT GGGGGCCCTG CCCAGCTCCA GGCCCTGGCC
CACAGGTGTC CCCCTTTGGC TGTGTGCTTG GCCCAGCAGC TGCCTGAGGA CCCAGGGCAA
GGCAGCAGCT CCGTGGAGTT TGACATGGTC AAGCTGGTGG ACTCCATGGG CTGGGAGCTG
GCCTCTGTGC GGCGGGCTCT CTGCCAGCTG CAGTGGGACC ACGAGCCCAG GACAGGTGTG
CGGCGTGGGA CAGGGGTGCT TGTGGAGTTC AGTGAGCTGG CCTTCCACCT TCGCAGCCCG
GGGGACCTGA CCGCTGAGGA GAAGGACCAG ATATGTGACT TCCTCTATGG CCGTGTGCAG
GCCCGGGAGC GCCAGGCCCT GGCCCGTCTG CGCAGAACCT TCCAGGCCTT TCACAGCGTA
GCCTTCCCCA GCTGCGGGCC CTGCCTGGAG CAGCAGGATG AGGAGCGCAG CACCAGGCTC
AAGGACCTGC TCGGCCGCTA CTTTGAGGAA GAGGAAGGGC AGGAGCCGGG AGGCATGGAG
GACGCACAGG GCCCCGAGCC AGGGCAGGCC AGACTCCAGG ATTGGGAGGA CCAGGTCCGC
TGCGACATCC GCCAGTTCCT GTCCCTGAGG CCAGAGGAGA AGTTCTCCAG CAGGGCTGTG
GCCCGCATCT TCCACGGCAT CGGAAGCCCC TGCTACCCGG CCCAGGTGTA CGGGCAGGAC
CGACGCTTCT GGAGAAAATA CCTGCACCTG AGCTTCCATG CCCTGGTGGG CCTGGCCACG
GAAGAGCTCC TGCAGGTGGC CCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu62803
Clone ID Related Accession (Same CDS sequence) XM_011517384.2
Accession Version XM_011517384.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product ATP-dependent DNA helicase Q4 isoform X10
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011517384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGGCGA AGACCTGCGA
GAGCTGCGCA GACATGTGCA CGCCGACAGC ACGGACTTCC TGGCTGTGAA GAGGCTGGTA
CAGCGCGTGT TCCCAGCCTG CACCTGCACC TGCACCAGGC CGCCCTCGGA GCAGGAAGGG
GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG TACCCCCCTC AAGAGGCTGA GCAGCTTAGC
CACCAAGCAG CCCCAGGACC CAGAAGGGTC TGCATGGGCC ATGAGCGGGC ACTCCCAATA
CAGCTTACCG TACAGGCTTT GGACATGCCG GAGGAGGCCA TCGAGACTTT GCTGTGCTAC
CTGGAGCTGC ACCCACACCA CTGGCTGGAG CTGCTGGCGA CCACCTATAC CCATTGCCGT
CTGAACTGCC CTGGGGGCCC TGCCCAGCTC CAGGCCCTGG CCCACAGGTG TCCCCCTTTG
GCTGTGTGCT TGGCCCAGCA GCTGCCTGAG GACCCAGGGC AAGGCAGCAG CTCCGTGGAG
TTTGACATGG TCAAGCTGGT GGACTCCATG GGCTGGGAGC TGGCCTCTGT GCGGCGGGCT
CTCTGCCAGC TGCAGTGGGA CCACGAGCCC AGGACAGGTG TGCGGCGTGG GACAGGGGTG
CTTGTGGAGT TCAGTGAGCT GGCCTTCCAC CTTCGCAGCC CGGGGGACTT GACCGCTGAG
GAGAAGGACC AGATATGTGA CTTCCTCTAT GGCCGTGTGC AGGCCCGGGA GCGCCAGGCC
CTGGCCCGTC TGCGCAGAAC CTTCCAGGCC TTTCACAGCG TAGCCTTCCC CAGCTGCGGG
CCCTGCCTGG AGCAGCAGGA TGAGGAGCGC AGCACCAGGC TCAAGGACCT GCTCGGCCGC
TACTTTGAGG AAGAGGAAGG GCAGGAGCCG GGAGGCATGG AGGACGCACA GGGCCCCGAG
CCAGGGCAGG CCAGACTCCA GGATTGGGAG GACCAGGTCC GCTGCGACAT CCGCCAGTTC
CTGTCCCTGA GGCCAGAGGA GAAGTTCTCC AGCAGGGCTG TGGCCCGCAT CTTCCACGGC
ATCGGAAGCC CCTGCTACCC GGCCCAGGTG TACGGGCAGG ACCGACGCTT CTGGAGAAAA
TACCTGCACC TGAGCTTCCA TGCCCTGGTG GGCCTGGCCA CGGAAGAGCT CCTGCAGGTG
GCCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011515686.1
CDS43..3471
Misc Feature(1)1459..2010
Misc Feature(2)1486..1980
Misc Feature(3)1555..1569
Misc Feature(4)1855..1866
Translation

Target ORF information:

RefSeq Version XM_011517384.2
Organism Homo sapiens(Human)
Definition Homo sapiens RecQ like helicase 4 (RECQL4), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011517384.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGGAGCGGC TGCGGGACGT GCGGGAGCGG CTGCAGGCGT GGGAGCGCGC GTTCCGACGG 
CAGCGCGGGC GGCGACCGAG CCAGGACGAC GTGGAGGCGG CGCCGGAGGA GACCCGCGCG
CTCTACCGGG AATACCGCAC TCTGAAGCGT ACCACGGGCC AGGCCGGCGG CGGGCTCCGC
AGCTCCGAGT CGCTCCCCGC GGCGGCCGAA GAGGCGCCAG AGCCCCGCTG CTGGGGGCCC
CATCTGAATC GGGCTGCGAC CAAGAGTCCA CAGTCTACGC CAGGGCGGAG CCGCCAGGGC
TCGGTGCCGG ACTACGGGCA GCGGCTCAAG GCCAATCTGA AAGGCACCCT GCAGGCCGGA
CCAGCCCTGG GCCGCAGACC GTGGCCTCTA GGAAGAGCCT CATCTAAGGC ATCCACCCCA
AAGCCCCCAG GTACAGGGCC TGTCCCCTCC TTTGCAGAAA AAGTCAGTGA TGAGCCTCCA
CAGCTCCCTG AGCCCCAGCC AAGGCCAGGC CGGCTCCAGC ATCTGCAGGC ATCCCTGAGC
CAGCGGCTGG GCTCCCTAGA TCCTGGCTGG TTACAGCGAT GTCACAGTGA GGTCCCAGAT
TTTCTGGGGG CCCCCAAAGC CTGCAGGCCT GATCTAGGCT CAGAGGAATC ACAACTTCTG
ATCCCTGGTG AGTCGGCTGT CCTTGGTCCT GGTGCTGGCT CCCAGGGCCC AGAGGCTTCA
GCCTTCCAAG AAGTCAGCAT CCGTGTGGGG AGCCCCCAGC CCAGCAGCAG TGGAGGCGAG
AAGCGGAGAT GGAACGAGGA GCCCTGGGAG AGCCCCGCAC AGGTCCAGCA GGAGAGCAGC
CAAGCTGGAC CCCCATCGGA GGGGGCTGGG GCTGTAGCAG TTGAGGAAGA CCCTCCAGGG
GAACCTGTAC AGGCACAGCC ACCTCAGCCC TGCAGCAGCC CATCGAACCC CAGGTACCAC
GGACTCAGCC CCTCCAGTCA AGCTAGGGCT GGGAAGGCTG AGGGCACAGC CCCCCTGCAC
ATCTTCCCTC GGCTGGCCCG CCATGACAGG GGCAATTACG TACGGCTCAA CATGAAGCAG
AAACACTACG TGCGGGGCCG GGCACTCCGT AGCAGGCTCC TCCGCAAGCA GGCATGGAAG
CAGAAGTGGC GGAAGAAAGG GGAGTGTTTT GGGGGTGGTG GTGCCACAGT CACAACCAAG
GAGTCTTGTT TCCTGAACGA GCAGTTCGAT CACTGGGCAG CCCAGTGTCC CCGGCCAGCA
AGTGAGGAAG ACACAGATGC TGTTGGGCCT GAGCCACTGG TTCCTTCACC ACAACCTGTA
CCTGAGGTGC CCAGCCTGGA CCCCACCGTG CTGCCACTCT ACTCCCTGGG GCCCTCAGGG
CAGTTGGCAG AGACGCCGGC TGAGGTGTTC CAGGCCCTGG AGCAGCTGGG GCACCAAGCC
TTTCGCCCTG GGCAGGAGCG TGCAGTCATG CGGATCCTGT CTGGCATCTC CACGCTGCTG
GTGCTGCCTA CAGGTGCCGG CAAGTCCCTG TGCTACCAGC TCCCAGCGCT GCTCTACAGC
CGGCGCAGCC CCTGCCTCAC GTTGGTCGTC TCTCCCCTGC TGTCACTCAT GGATGACCAG
GTGTCTGGCC TGCCACCGTG TCTCAAGGCG GCCTGCATAC ACTCGGGCAT GACCAGGAAG
CAACGGGAAT CTGTCCTGCA GAAGATTCGG GCAGCCCAGG TACACGTGCT GATGCTGACA
CCTGAGGCAC TGGTGGGGGC GGGAGGCCTC CCTCCAGCCG CACAGCTGCC TCCAGTTGCT
TTTGCCTGCA TTGATGAGGC CCACTGCCTC TCCCAGTGGT CCCACAACTT CCGGCCCTGC
TACCTGCGCG TCTGCAAGGT GCTTCGGGAG CGCATGGGCG TGCACTGCTT CCTGGGCCTC
ACAGCCACAG CCACACGCCG CACTGCCAGT GACGTGGCAC AGCACCTGGC TGTGGCTGAA
GAGCCTGACC TCCACGGGCC AGCCCCAGTT CCCACCAACC TGCACCTTTC CGTGTCCATG
GACAGGGACA CAGACCAGGC ACTGTTGACG CTGCTGCAAG GCAAACGTTT TCAAAACCTC
GATTCCATTA TCATTTACTG CAACCGGCGC GAGGACACAG AGCGGATCGC TGCGCTCCTC
CGAACCTGCC TGCACGCAGC CTGGGTCCCA GGGTCTGGAG GTCGTGCCCC CAAAACCACA
GCCGAGGCCT ACCACGCGGG CATGTGCAGC CGGGAACGGC GGCGGGGCGA AGACCTGCGA
GAGCTGCGCA GACATGTGCA CGCCGACAGC ACGGACTTCC TGGCTGTGAA GAGGCTGGTA
CAGCGCGTGT TCCCAGCCTG CACCTGCACC TGCACCAGGC CGCCCTCGGA GCAGGAAGGG
GCCGTGGGTG GGGAGAGGCC TGTGCCCAAG TACCCCCCTC AAGAGGCTGA GCAGCTTAGC
CACCAAGCAG CCCCAGGACC CAGAAGGGTC TGCATGGGCC ATGAGCGGGC ACTCCCAATA
CAGCTTACCG TACAGGCTTT GGACATGCCG GAGGAGGCCA TCGAGACTTT GCTGTGCTAC
CTGGAGCTGC ACCCACACCA CTGGCTGGAG CTGCTGGCGA CCACCTATAC CCATTGCCGT
CTGAACTGCC CTGGGGGCCC TGCCCAGCTC CAGGCCCTGG CCCACAGGTG TCCCCCTTTG
GCTGTGTGCT TGGCCCAGCA GCTGCCTGAG GACCCAGGGC AAGGCAGCAG CTCCGTGGAG
TTTGACATGG TCAAGCTGGT GGACTCCATG GGCTGGGAGC TGGCCTCTGT GCGGCGGGCT
CTCTGCCAGC TGCAGTGGGA CCACGAGCCC AGGACAGGTG TGCGGCGTGG GACAGGGGTG
CTTGTGGAGT TCAGTGAGCT GGCCTTCCAC CTTCGCAGCC CGGGGGACTT GACCGCTGAG
GAGAAGGACC AGATATGTGA CTTCCTCTAT GGCCGTGTGC AGGCCCGGGA GCGCCAGGCC
CTGGCCCGTC TGCGCAGAAC CTTCCAGGCC TTTCACAGCG TAGCCTTCCC CAGCTGCGGG
CCCTGCCTGG AGCAGCAGGA TGAGGAGCGC AGCACCAGGC TCAAGGACCT GCTCGGCCGC
TACTTTGAGG AAGAGGAAGG GCAGGAGCCG GGAGGCATGG AGGACGCACA GGGCCCCGAG
CCAGGGCAGG CCAGACTCCA GGATTGGGAG GACCAGGTCC GCTGCGACAT CCGCCAGTTC
CTGTCCCTGA GGCCAGAGGA GAAGTTCTCC AGCAGGGCTG TGGCCCGCAT CTTCCACGGC
ATCGGAAGCC CCTGCTACCC GGCCCAGGTG TACGGGCAGG ACCGACGCTT CTGGAGAAAA
TACCTGCACC TGAGCTTCCA TGCCCTGGTG GGCCTGGCCA CGGAAGAGCT CCTGCAGGTG
GCCCGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Mitochondrial functions of RECQL4 are required for the prevention of aerobic glycolysis-dependent cell invasion.
Journal of cell science129(7)1312-8(2016 Apr)
Kumari J,Hussain M,De S,Chandra S,Modi P,Tikoo S,Singh A,Sagar C,Sepuri NB,Sengupta S


book

RECQL4 helicase has oncogenic potential in sporadic breast cancers.
The Journal of pathology238(4)495-501(2016 Mar)
Arora A,Agarwal D,Abdel-Fatah TM,Lu H,Croteau DL,Moseley P,Aleskandarany MA,Green AR,Ball G,Rakha EA,Chan SY,Ellis IO,Wang LL,Zhao Y,Balajee AS,Bohr VA,Madhusudan S


book

Structural and biochemical characterization of an RNA/DNA binding motif in the N-terminal domain of RecQ4 helicases.
Scientific reports621501(2016 Feb)
Marino F,Mojumdar A,Zucchelli C,Bhardwaj A,Buratti E,Vindigni A,Musco G,Onesti S


book

RecQL4 is required for the association of Mcm10 and Ctf4 with replication origins in human cells.
Cell cycle (Georgetown, Tex.)14(7)1001-9(2015)
Im JS,Park SY,Cho WH,Bae SH,Hurwitz J,Lee JK


 
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