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MAGED1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MAGED1
Entrez Gene ID 9500
Full Name MAGE family member D1
Synonyms DLXIN-1,NRAGE
General protein information
Preferred Names

MAGE family member D1

Names

melanoma-associated antigen D1
MAGE tumor antigen CCF
MAGE-D1 antigen
melanoma antigen family D, 1
melanoma antigen family D1
neurotrophin receptor-interacting MAGE homolog

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xp11.22

Summary This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

300224


mRNA and Protein(s)

mRNA Protein Name
NM_006986.3 NP_008917.3 melanoma-associated antigen D1 isoform b
XM_017029978.1 XP_016885467.1 melanoma-associated antigen D1 isoform X1
XM_011530835.2 XP_011529137.1 melanoma-associated antigen D1 isoform X1
NM_001005333.1 NP_001005333.1 melanoma-associated antigen D1 isoform a
XM_017029979.1 XP_016885468.1 melanoma-associated antigen D1 isoform X1
XM_011530835.1 XP_011529137.1 melanoma-associated antigen D1 isoform X1
NM_001005332.1 NP_001005332.1 melanoma-associated antigen D1 isoform b
XM_017029980.1 XP_016885469.1 melanoma-associated antigen D1 isoform X1



Macaca mulatta (rhesus monkey) LOC696658 XP_002808576.1
Mus musculus (house mouse) Maged1 NP_062765.1
Rattus norvegicus (Norway rat) Maged1 NP_445861.1
Canis lupus familiaris (dog) MAGED1 XP_005641419.1
Bos taurus (cattle) MAGED1 NP_001039590.2
Homo sapiens (human) MAGED1 NP_001005333.1
Pan troglodytes (chimpanzee) MAGED1 XP_003317513.1


Related articles in PubMed

Xp11.22 deletions encompassing CENPVL1, CENPVL2, MAGED1 and GSPT2 as a cause of syndromic X-linked intellectual disability.
Grau C, Starkovich M, Azamian MS, Xia F, Cheung SW, Evans P, Henderson A, Lalani SR, Scott DA
PloS one12(4)e0175962(2017)

Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells.
Zhou H, Zhang G, Xue X, Yang Y, Yang Y, Chang X, Ge X, Xiao Z, Guo H, Wang Y
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(7)8741-52(2016 Jul)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kovács IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barabási AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

NRAGE promotes cell proliferation by stabilizing PCNA in a ubiquitin-proteasome pathway in esophageal carcinomas.
Yang Q, Ou C, Liu M, Xiao W, Wen C, Sun F
Carcinogenesis35(7)1643-51(2014 Jul)

The roles of MAGE-D1 in the neuronal functions and pathology of the central nervous system.
Mouri A, Noda Y, Watanabe K, Nabeshima T
Reviews in the neurosciences24(1)61-70(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results demonstrate the effective anti-autophagic of NRAGE in non-small-cell lung cancer cells through AMPK/Ulk1/Atg13 autophagy signaling pathways. Therefore, NRAGE could be used as a potential therapeutic target for lung cancer.
Title: Silencing of NRAGE induces autophagy via AMPK/Ulk1/Atg13 signaling pathway in NSCLC cells.

our surprise, NRAGE induces nuclear localization of beta-catenin and increases its DNA binding ability. Further studies reveal that NRAGE leads to the modification of beta-catenin/Arm with O-linked beta-N-acetylglucosamine (O-GlcNAc), and failure of the association between beta-catenin/Arm and pygopus(pygo) protein, which is required for transcriptional activation of Wnt target genes. Therefore, our findings suggest a ...
Title: NRAGE induces β-catenin/Arm O-GlcNAcylation and negatively regulates Wnt signaling.

Loss of GSPT2 and/or MAGED1 function may contribute to the intellectual disability.
Title: Xp11.22 deletions encompassing CENPVL1, CENPVL2, MAGED1 and GSPT2 as a cause of syndromic X-linked intellectual disability.

MAGED1 binds and positively regulates the transcriptional activity of family members SIM1, SIM2, NPAS4 and ARNT2, but does not interact with AhR, HIF1alpha and ARNT. This interaction is mediated by PAS repeat regions which also form the interface for bHLH PAS dimerisation, and accordingly MAGED1 is not found in complex with bHLH PAS dimers.
Title: MAGED1 is a novel regulator of a select subset of bHLH PAS transcription factors.

The ectopic subcellular localization of NRAGE mediated nuclear translocation of beta-catenin.
Title: Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells.

The following MAGED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu03665
NM_001005333.1
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$339.50-$475.30
$679.00
OHu18181
NM_006986.3
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu18181
NM_001005332.1
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu18181
XM_017029978.1
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu18181
XM_011530835.2
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu18181
XM_017029980.1
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu18181
XM_017029979.1
Latest version!
Homo sapiens MAGE family member D1 (MAGED1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
View Old Accession Versions >>
OHu18181
XM_011530835.1 Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu03665
Clone ID Related Accession (Same CDS sequence) NM_001005333.1
Accession Version NM_001005333.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen D1 isoform a
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX422007.2, BC014070.2 and AF132205.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014070.2, SRR1660809.249174.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGAATCC TGACGCTTGT 
AGAGCAGTCT GTCACCCCCT CCCCCAGCCT CCTGCCTCCA CCCTCCCTCT TTCTGCATTC
CCCACTCTGT GCGACCCCCC TTATTCTCAA CTCCGCGATC CGCCTGCCGT CCTGAGCTGC
TACTGCACGC CTCTTGGAGC GTCCCCGGCT CCTGCTGAGG CCTCCGTAGA AGACAGCGCC
TTGCTTATGC AGACCTTGAT GGAGGCCATC CAGATCTCAG AGGCTCCACC TACTAACCAG
GCCACCGCAG CTGCTAGTCC CCAGAGTTCA CAGCCCCCAA CTGCCAATGA GATGGCTGAC
ATTCAGGTTT CAGCAGCTGC CGCTAGGCCT AAGTCAGCCT TTAAAGTCCA GAATGCCACC
ACAAAAGGCC CAAATGGTGT CTATGATTTC TCTCAGGCTC ATAATGCCAA GGATGTGCCC
AACACGCAGC CCAAGGCAGC CTTTAAGTCC CAAAATGCTA CCCCAAAGGG TCCAAATGCT
GCCTATGATT TTTCCCAGGC AGCAACCACT GGTGAGTTAG CTGCTAACAA GTCTGAGATG
GCCTTCAAGG CCCAGAATGC CACTACTAAA GTGGGCCCAA ATGCCACCTA CAATTTCTCT
CAGTCTCTCA ATGCCAATGA CCTGGCCAAC AGCAGGCCTA AGACCCCTTT CAAGGCTTGG
AATGATACCA CTAAGGCCCC AACAGCTGAT ACCCAGACCC AGAATGTAAA TCAGGCCAAA
ATGGCCACTT CCCAGGCTGA CATAGAGACC GACCCAGGTA TCTCTGAACC TGACGGTGCA
ACTGCACAGA CATCAGCAGA TGGTTCCCAG GCTCAGAATC TGGAGTCCCG GACAATAATT
CGGGGCAAGA GGACCCGCAA GATTAATAAC TTGAATGTTG AAGAGAACAG CAGTGGGGAT
CAGAGGCGGG CCCCACTGGC TGCAGGGACC TGGAGGTCTG CACCAGTTCC AGTGACCACT
CAGAACCCAC CTGGCGCACC CCCCAATGTG CTCTGGCAGA CGCCATTGGC TTGGCAGAAC
CCCTCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCACGTCA GAGCCCTCCA
GCTAGGCAGA CCCCACCAGC CTGGCAGAAC CCAGTCGCTT GGCAGAACCC AGTGATTTGG
CCAAACCCAG TAATCTGGCA GAACCCAGTG ATCTGGCCAA ACCCCATTGT CTGGCCCGGC
CCTGTTGTCT GGCCGAATCC ACTGGCCTGG CAGAATCCAC CTGGATGGCA GACTCCACCT
GGATGGCAGA CCCCACCGGG CTGGCAGGGT CCTCCAGACT GGCAAGGTCC TCCTGACTGG
CCGCTACCAC CCGACTGGCC ACTGCCACCT GATTGGCCAC TTCCCACTGA CTGGCCACTA
CCACCTGACT GGATCCCCGC TGATTGGCCA ATTCCACCTG ACTGGCAGAA CCTGCGCCCC
TCGCCTAACC TGCGCCCTTC TCCCAACTCG CGTGCCTCAC AGAACCCAGG TGCTGCACAG
CCCCGAGATG TGGCCCTTCT TCAGGAAAGA GCAAATAAGT TGGTCAAGTA CTTGATGCTT
AAGGACTACA CAAAGGTGCC CATCAAGCGC TCAGAAATGC TGAGAGATAT CATCCGTGAA
TACACTGATG TTTATCCAGA AATCATTGAA CGTGCATGCT TTGTCCTAGA GAAGAAATTT
GGGATTCAAC TGAAAGAAAT TGACAAAGAA GAACACCTGT ATATTCTCAT CAGTACCCCC
GAGTCCCTGG CTGGCATACT GGGAACGACC AAAGACACAC CCAAGCTCGG TCTCCTCTTG
GTGATTCTGG GTGTCATCTT CATGAATGGC AACCGTGCCA GTGAGGCTGT CCTCTGGGAG
GCACTACGCA AGATGGGACT GCGTCCTGGG GTGAGACATC CCCTCCTTGG AGATCTAAGG
AAACTTCTCA CCTATGAGTT TGTAAAGCAG AAATACCTGG ACTACAGACG AGTGCCCAAC
AGCAACCCCC CGGAGTATGA GTTCCTCTGG GGCCTCCGTT CCTACCATGA GACTAGCAAG
ATGAAAGTGC TGAGATTCAT TGCAGAGGTT CAGAAAAGAG ACCCTCGTGA CTGGACTGCA
CAGTTCATGG AGGCTGCAGA TGAGGCCTTG GATGCTCTGG ATGCTGCTGC AGCTGAGGCC
GAAGCCCGGG CTGAAGCAAG AACCCGCATG GGAATTGGAG ATGAGGCTGT GTCTGGGCCC
TGGAGCTGGG ATGACATTGA GTTTGAGCTG CTGACCTGGG ATGAGGAAGG AGATTTTGGA
GATCCCTGGT CCAGAATTCC ATTTACCTTC TGGGCCAGAT ACCACCAGAA TGCCCGCTCC
AGATTCCCTC AGACCTTTGC CGGTCCCATT ATTGGTCCTG GTGGTACAGC CAGTGCCAAC
TTCGCTGCCA ACTTTGGTGC CATTGGTTTC TTCTGGGTTG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001005333.1
CDS191..2695
Misc Feature(1)146..148
Misc Feature(2)632..634
Misc Feature(3)632..634
Misc Feature(4)1790..2272
Exon (1)1..154
Exon (2)155..235
Exon (3)236..403
Exon (4)404..1111
Exon (5)1112..1780
Exon (6)1781..1844
Exon (7)1845..1924
Exon (8)1925..2016
Exon (9)2017..2096
Exon (10)2097..2139
Exon (11)2140..2202
Exon (12)2203..2317
Exon (13)2318..2703
Exon (14)2704..2912
Translation

Target ORF information:

RefSeq Version NM_001005333.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001005333.1

ORF Insert Sequence:

1
61
121
181
241
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361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGAATCC TGACGCTTGT 
AGAGCAGTCT GTCACCCCCT CCCCCAGCCT CCTGCCTCCA CCCTCCCTCT TTCTGCATTC
CCCACTCTGT GCGACCCCCC TTATTCTCAA CTCCGCGATC CGCCTGCCGT CCTGAGCTGC
TACTGCACGC CTCTTGGAGC GTCCCCGGCT CCTGCTGAGG CCTCCGTAGA AGACAGCGCC
TTGCTTATGC AGACCTTGAT GGAGGCCATC CAGATCTCAG AGGCTCCACC TACTAACCAG
GCCACCGCAG CTGCTAGTCC CCAGAGTTCA CAGCCCCCAA CTGCCAATGA GATGGCTGAC
ATTCAGGTTT CAGCAGCTGC CGCTAGGCCT AAGTCAGCCT TTAAAGTCCA GAATGCCACC
ACAAAAGGCC CAAATGGTGT CTATGATTTC TCTCAGGCTC ATAATGCCAA GGATGTGCCC
AACACGCAGC CCAAGGCAGC CTTTAAGTCC CAAAATGCTA CCCCAAAGGG TCCAAATGCT
GCCTATGATT TTTCCCAGGC AGCAACCACT GGTGAGTTAG CTGCTAACAA GTCTGAGATG
GCCTTCAAGG CCCAGAATGC CACTACTAAA GTGGGCCCAA ATGCCACCTA CAATTTCTCT
CAGTCTCTCA ATGCCAATGA CCTGGCCAAC AGCAGGCCTA AGACCCCTTT CAAGGCTTGG
AATGATACCA CTAAGGCCCC AACAGCTGAT ACCCAGACCC AGAATGTAAA TCAGGCCAAA
ATGGCCACTT CCCAGGCTGA CATAGAGACC GACCCAGGTA TCTCTGAACC TGACGGTGCA
ACTGCACAGA CATCAGCAGA TGGTTCCCAG GCTCAGAATC TGGAGTCCCG GACAATAATT
CGGGGCAAGA GGACCCGCAA GATTAATAAC TTGAATGTTG AAGAGAACAG CAGTGGGGAT
CAGAGGCGGG CCCCACTGGC TGCAGGGACC TGGAGGTCTG CACCAGTTCC AGTGACCACT
CAGAACCCAC CTGGCGCACC CCCCAATGTG CTCTGGCAGA CGCCATTGGC TTGGCAGAAC
CCCTCAGGCT GGCAAAACCA GACAGCCAGG CAGACCCCAC CAGCACGTCA GAGCCCTCCA
GCTAGGCAGA CCCCACCAGC CTGGCAGAAC CCAGTCGCTT GGCAGAACCC AGTGATTTGG
CCAAACCCAG TAATCTGGCA GAACCCAGTG ATCTGGCCAA ACCCCATTGT CTGGCCCGGC
CCTGTTGTCT GGCCGAATCC ACTGGCCTGG CAGAATCCAC CTGGATGGCA GACTCCACCT
GGATGGCAGA CCCCACCGGG CTGGCAGGGT CCTCCAGACT GGCAAGGTCC TCCTGACTGG
CCGCTACCAC CCGACTGGCC ACTGCCACCT GATTGGCCAC TTCCCACTGA CTGGCCACTA
CCACCTGACT GGATCCCCGC TGATTGGCCA ATTCCACCTG ACTGGCAGAA CCTGCGCCCC
TCGCCTAACC TGCGCCCTTC TCCCAACTCG CGTGCCTCAC AGAACCCAGG TGCTGCACAG
CCCCGAGATG TGGCCCTTCT TCAGGAAAGA GCAAATAAGT TGGTCAAGTA CTTGATGCTT
AAGGACTACA CAAAGGTGCC CATCAAGCGC TCAGAAATGC TGAGAGATAT CATCCGTGAA
TACACTGATG TTTATCCAGA AATCATTGAA CGTGCATGCT TTGTCCTAGA GAAGAAATTT
GGGATTCAAC TGAAAGAAAT TGACAAAGAA GAACACCTGT ATATTCTCAT CAGTACCCCC
GAGTCCCTGG CTGGCATACT GGGAACGACC AAAGACACAC CCAAGCTCGG TCTCCTCTTG
GTGATTCTGG GTGTCATCTT CATGAATGGC AACCGTGCCA GTGAGGCTGT CCTCTGGGAG
GCACTACGCA AGATGGGACT GCGTCCTGGG GTGAGACATC CCCTCCTTGG AGATCTAAGG
AAACTTCTCA CCTATGAGTT TGTAAAGCAG AAATACCTGG ACTACAGACG AGTGCCCAAC
AGCAACCCCC CGGAGTATGA GTTCCTCTGG GGCCTCCGTT CCTACCATGA GACTAGCAAG
ATGAAAGTGC TGAGATTCAT TGCAGAGGTT CAGAAAAGAG ACCCTCGTGA CTGGACTGCA
CAGTTCATGG AGGCTGCAGA TGAGGCCTTG GATGCTCTGG ATGCTGCTGC AGCTGAGGCC
GAAGCCCGGG CTGAAGCAAG AACCCGCATG GGAATTGGAG ATGAGGCTGT GTCTGGGCCC
TGGAGCTGGG ATGACATTGA GTTTGAGCTG CTGACCTGGG ATGAGGAAGG AGATTTTGGA
GATCCCTGGT CCAGAATTCC ATTTACCTTC TGGGCCAGAT ACCACCAGAA TGCCCGCTCC
AGATTCCCTC AGACCTTTGC CGGTCCCATT ATTGGTCCTG GTGGTACAGC CAGTGCCAAC
TTCGCTGCCA ACTTTGGTGC CATTGGTTTC TTCTGGGTTG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18181
Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , XM_011530835.1 , NM_001005332.1 , XM_017029980.1
Accession Version NM_006986.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen D1 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX422007.2, CD387872.1, BF529661.1, BC014070.2 and AF132205.1. On Sep 24, 2004 this sequence version replaced NM_006986.2. Transcript Variant: This variant (2) lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF258554.1, SRR1803612.173417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
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361
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481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_008917.3
CDS191..2527
Misc Feature(1)146..148
Misc Feature(2)464..466
Misc Feature(3)464..466
Misc Feature(4)464..466
Misc Feature(5)1076..1522
Misc Feature(6)1622..2104
Exon (1)1..154
Exon (2)155..235
Exon (3)236..943
Exon (4)944..1612
Exon (5)1613..1676
Exon (6)1677..1756
Exon (7)1757..1848
Exon (8)1849..1928
Exon (9)1929..1971
Exon (10)1972..2034
Exon (11)2035..2149
Exon (12)2150..2535
Exon (13)2536..2744
Translation

Target ORF information:

RefSeq Version NM_006986.3
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006986.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18181
Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , XM_011530835.1 , NM_001005332.1 , XM_017029980.1
Accession Version NM_001005332.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen D1 isoform b
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG819042.1, BC014070.2 and AF132205.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame coding exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Variants 2 and 3 both encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001005332.1
CDS96..2432
Misc Feature(1)369..371
Misc Feature(2)369..371
Misc Feature(3)369..371
Misc Feature(4)981..1427
Misc Feature(5)1527..2009
Exon (1)1..59
Exon (2)60..140
Exon (3)141..848
Exon (4)849..1517
Exon (5)1518..1581
Exon (6)1582..1661
Exon (7)1662..1753
Exon (8)1754..1833
Exon (9)1834..1876
Exon (10)1877..1939
Exon (11)1940..2054
Exon (12)2055..2440
Exon (13)2441..2649
Translation

Target ORF information:

RefSeq Version NM_001005332.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001005332.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18181
Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , XM_011530835.1 , NM_001005332.1 , XM_017029980.1
Accession Version XM_017029978.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen D1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016885467.1
CDS106..2442
Misc Feature(1)1537..2019
Translation

Target ORF information:

RefSeq Version XM_017029978.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017029978.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18181
Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , XM_011530835.1 , NM_001005332.1 , XM_017029980.1
Accession Version XM_011530835.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen D1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529137.1
CDS214..2550
Misc Feature(1)1645..2127
Translation

Target ORF information:

RefSeq Version XM_011530835.2
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530835.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18181
Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , XM_011530835.1 , NM_001005332.1 , XM_017029980.1
Accession Version XM_017029979.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen D1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016885468.1
CDS792..3128
Misc Feature(1)2223..2705
Translation

Target ORF information:

RefSeq Version XM_017029979.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017029979.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18181
Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , XM_011530835.1 , NM_001005332.1 , XM_017029980.1
Accession Version XM_011530835.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product melanoma-associated antigen D1 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529137.1
Translation

Target ORF information:

RefSeq Version XM_011530835.1
Organism Homo sapiens(Human)
Definition Homo sapiens melanoma antigen family D1 (MAGED1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011530835.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18181
Clone ID Related Accession (Same CDS sequence) NM_006986.3 , XM_017029978.1 , XM_011530835.2 , XM_017029979.1 , XM_011530835.1 , NM_001005332.1 , XM_017029980.1
Accession Version XM_017029980.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen D1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016885469.1
CDS610..2946
Misc Feature(1)2041..2523
Translation

Target ORF information:

RefSeq Version XM_017029980.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member D1 (MAGED1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017029980.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGGCTCAGA AAATGGACTG TGGTGCGGGC CTCCTCGGCT TCCAGGCTGA GGCCTCCGTA 
GAAGACAGCG CCTTGCTTAT GCAGACCTTG ATGGAGGCCA TCCAGATCTC AGAGGCTCCA
CCTACTAACC AGGCCACCGC AGCTGCTAGT CCCCAGAGTT CACAGCCCCC AACTGCCAAT
GAGATGGCTG ACATTCAGGT TTCAGCAGCT GCCGCTAGGC CTAAGTCAGC CTTTAAAGTC
CAGAATGCCA CCACAAAAGG CCCAAATGGT GTCTATGATT TCTCTCAGGC TCATAATGCC
AAGGATGTGC CCAACACGCA GCCCAAGGCA GCCTTTAAGT CCCAAAATGC TACCCCAAAG
GGTCCAAATG CTGCCTATGA TTTTTCCCAG GCAGCAACCA CTGGTGAGTT AGCTGCTAAC
AAGTCTGAGA TGGCCTTCAA GGCCCAGAAT GCCACTACTA AAGTGGGCCC AAATGCCACC
TACAATTTCT CTCAGTCTCT CAATGCCAAT GACCTGGCCA ACAGCAGGCC TAAGACCCCT
TTCAAGGCTT GGAATGATAC CACTAAGGCC CCAACAGCTG ATACCCAGAC CCAGAATGTA
AATCAGGCCA AAATGGCCAC TTCCCAGGCT GACATAGAGA CCGACCCAGG TATCTCTGAA
CCTGACGGTG CAACTGCACA GACATCAGCA GATGGTTCCC AGGCTCAGAA TCTGGAGTCC
CGGACAATAA TTCGGGGCAA GAGGACCCGC AAGATTAATA ACTTGAATGT TGAAGAGAAC
AGCAGTGGGG ATCAGAGGCG GGCCCCACTG GCTGCAGGGA CCTGGAGGTC TGCACCAGTT
CCAGTGACCA CTCAGAACCC ACCTGGCGCA CCCCCCAATG TGCTCTGGCA GACGCCATTG
GCTTGGCAGA ACCCCTCAGG CTGGCAAAAC CAGACAGCCA GGCAGACCCC ACCAGCACGT
CAGAGCCCTC CAGCTAGGCA GACCCCACCA GCCTGGCAGA ACCCAGTCGC TTGGCAGAAC
CCAGTGATTT GGCCAAACCC AGTAATCTGG CAGAACCCAG TGATCTGGCC AAACCCCATT
GTCTGGCCCG GCCCTGTTGT CTGGCCGAAT CCACTGGCCT GGCAGAATCC ACCTGGATGG
CAGACTCCAC CTGGATGGCA GACCCCACCG GGCTGGCAGG GTCCTCCAGA CTGGCAAGGT
CCTCCTGACT GGCCGCTACC ACCCGACTGG CCACTGCCAC CTGATTGGCC ACTTCCCACT
GACTGGCCAC TACCACCTGA CTGGATCCCC GCTGATTGGC CAATTCCACC TGACTGGCAG
AACCTGCGCC CCTCGCCTAA CCTGCGCCCT TCTCCCAACT CGCGTGCCTC ACAGAACCCA
GGTGCTGCAC AGCCCCGAGA TGTGGCCCTT CTTCAGGAAA GAGCAAATAA GTTGGTCAAG
TACTTGATGC TTAAGGACTA CACAAAGGTG CCCATCAAGC GCTCAGAAAT GCTGAGAGAT
ATCATCCGTG AATACACTGA TGTTTATCCA GAAATCATTG AACGTGCATG CTTTGTCCTA
GAGAAGAAAT TTGGGATTCA ACTGAAAGAA ATTGACAAAG AAGAACACCT GTATATTCTC
ATCAGTACCC CCGAGTCCCT GGCTGGCATA CTGGGAACGA CCAAAGACAC ACCCAAGCTC
GGTCTCCTCT TGGTGATTCT GGGTGTCATC TTCATGAATG GCAACCGTGC CAGTGAGGCT
GTCCTCTGGG AGGCACTACG CAAGATGGGA CTGCGTCCTG GGGTGAGACA TCCCCTCCTT
GGAGATCTAA GGAAACTTCT CACCTATGAG TTTGTAAAGC AGAAATACCT GGACTACAGA
CGAGTGCCCA ACAGCAACCC CCCGGAGTAT GAGTTCCTCT GGGGCCTCCG TTCCTACCAT
GAGACTAGCA AGATGAAAGT GCTGAGATTC ATTGCAGAGG TTCAGAAAAG AGACCCTCGT
GACTGGACTG CACAGTTCAT GGAGGCTGCA GATGAGGCCT TGGATGCTCT GGATGCTGCT
GCAGCTGAGG CCGAAGCCCG GGCTGAAGCA AGAACCCGCA TGGGAATTGG AGATGAGGCT
GTGTCTGGGC CCTGGAGCTG GGATGACATT GAGTTTGAGC TGCTGACCTG GGATGAGGAA
GGAGATTTTG GAGATCCCTG GTCCAGAATT CCATTTACCT TCTGGGCCAG ATACCACCAG
AATGCCCGCT CCAGATTCCC TCAGACCTTT GCCGGTCCCA TTATTGGTCC TGGTGGTACA
GCCAGTGCCA ACTTCGCTGC CAACTTTGGT GCCATTGGTT TCTTCTGGGT TGAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Xp11.22 deletions encompassing CENPVL1, CENPVL2, MAGED1 and GSPT2 as a cause of syndromic X-linked intellectual disability.
PloS one12(4)e0175962(2017)
Grau C,Starkovich M,Azamian MS,Xia F,Cheung SW,Evans P,Henderson A,Lalani SR,Scott DA


book

Identification of novel NRAGE involved in the radioresistance of esophageal cancer cells.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(7)8741-52(2016 Jul)
Zhou H,Zhang G,Xue X,Yang Y,Yang Y,Chang X,Ge X,Xiao Z,Guo H,Wang Y


book

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kovács IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barabási AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M


book

NRAGE promotes cell proliferation by stabilizing PCNA in a ubiquitin-proteasome pathway in esophageal carcinomas.
Carcinogenesis35(7)1643-51(2014 Jul)
Yang Q,Ou C,Liu M,Xiao W,Wen C,Sun F


book

The roles of MAGE-D1 in the neuronal functions and pathology of the central nervous system.
Reviews in the neurosciences24(1)61-70(2013)
Mouri A,Noda Y,Watanabe K,Nabeshima T


 
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