LOC100215454 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100215454
Entrez Gene ID	100215454
Full Name	neuronal acetylcholine receptor subunit alpha-2-like
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_004208607.2	XP_004208655.2	neuronal acetylcholine receptor subunit alpha-2-like

Pathways from BioSystems
Gene Ontology

The following LOC100215454 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100215454 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc18440	XM_004208607.2 Latest version!	Hydra vulgaris neuronal acetylcholine receptor subunit alpha-2-like (LOC100215454), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100215454 ( XM_004208607.2 ) cDNA ORF clone, Hydra vulgaris -> XP_004208655.2 Hydra vulgaris neuronal acetylcholine receptor subunit alpha-2-like (LOC100215454), mRNA.

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CloneID	OHc18440
Clone ID Related Accession (Same CDS sequence)	XM_004208607.2
Accession Version	XM_004208607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-07
Organism	Hydra vulgaris
Product	neuronal acetylcholine receptor subunit alpha-2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004168736.1) and transcript sequence (GAOL01018783.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 8, 2015 this sequence version replaced XM_004208607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 155 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAAAATCA TTATTTTGAT TGTTACATAT TTATTCCAGT TTATAGAAAG TAGTCGTGAT 
GAAGAAAGAC TTTTAGATGA TTTGTTTAAA ACATATAATC CTGCTGCACG TCCAGTCTTG 
GATGATTCAG AATCAGTTAA TGTGACATTT GGGTTAACAT TACGACAAAT AATTGATGTG 
CATGAGAAAA TTCAAATACT TGTTATCAGT GCATGGATTA GACAGAAATG GAGAAATCCA 
ATCTTGACAT GGGATCCAAC AAAATATAAC AACATAAAAG AAATAAATGT AGATCCTTCA 
AGTATATGGC GGCCTGATAT TGTATTATAT AATAATGCAG ATGAAGATAA GACCTTTGGA 
GGTAATTTAG AAAGACTAAA AACAAGAGCT ATATTAAATT ATAATGGTGA CACTGCTTGG 
TTAGCTCCTG TCATTTTGAA GAGCAAATGT GATATTGATG TTAAGTATTT TCCTTTTGAT 
ACACAAAAGT GTCCTCTAAA ATTTGGCAGT TGGACATATG ACCAAGGAAG ATTAAATTTA 
ATTAACGAAA GCAATACAGC AACTTTAGAA AAATATTCAA ACAACTCTGA GTGGTATCTG 
CTAAGCGCAA AAGCAGTTCG AAATGAAGAA AAGTATTTCT GTTGTCCTGA ACTCTACCCT 
GATGTCACAT TTACGATTAT TATAGCTCGG CGATCTTTGT TTTACTTGTG TAATTTAATA 
TTCCCCATGA CAATAATTGG AATGTTAACT ATGTTATCAT TTTTATTGCC TGCCGAATCA 
GGCGAGAGAG TTTCACTTGC TATTACCCTT CTTCTTGCAA TGACTGTGTT CATGTTAGTT 
GTAGCTGATA TTATACCCGC TACAAGTGAT GTTATTCCAT TAGTTGGCAT TTATTTTAGT 
GTTTCAATGA TTGAAATGGT TATAATGATC ATTGTACTTT GTTACATCAT GAGGCTACAC 
AACAAGGGTC CAGGAGATCC GCCAATGCCT ATTTGGATGA GAAGATGGAT TTTGGAAAAC 
TTGTCGTACA AAGTTGGAAT TCGCGTACGA AGTGATCCGG ATCCTATGCA AATAAATAAT 
CAAAAGCTAA ATAACGACGA AAGTTCCATT CCTCTTAATA ATTTAAAGAT AAAGCAGTCC 
GAAATCAACT GGCAAAACAA AGTTTCAAAA TGTGAATTAT TACAATGCAA TGACAGCACT 
TTAAATAGTA CGCACAAAAA AAATAAGGCC ACTGTTCAAC TATCGGATTC AGAGCTTTTA 
TTGAGCAAAA TAGATGTTTT GCTGGAAAAG TTGCGTTCAA TGGAAGAAGA AGGAACAATA 
AAATCTGATT GGAGAATAGT AGCTATGACA ATTGATCGTT GCCTGCTGAT TTTTTTCGCA 
TTAATGGTGT TAATAACTCT ATTTGGTTGC TTCTCAATAT CACCAGGATA TGTTCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004208655.2
CDS	116..1555
Translation	MKIIILIVTYLFQFIESSRDEERLLDDLFKTYNPAARPVLDDSESVNVTFGLTLRQIIDVHEKIQILVISAWIRQKWRNPILTWDPTKYNNIKEINVDPSSIWRPDIVLYNNADEDKTFGGNLERLKTRAILNYNGDTAWLAPVILKSKCDIDVKYFPFDTQKCPLKFGSWTYDQGRLNLINESNTATLEKYSNNSEWYLLSAKAVRNEEKYFCCPELYPDVTFTIIIARRSLFYLCNLIFPMTIIGMLTMLSFLLPAESGERVSLAITLLLAMTVFMLVVADIIPATSDVIPLVGIYFSVSMIEMVIMIIVLCYIMRLHNKGPGDPPMPIWMRRWILENLSYKVGIRVRSDPDPMQINNQKLNNDESSIPLNNLKIKQSEINWQNKVSKCELLQCNDSTLNSTHKKNKATVQLSDSELLLSKIDVLLEKLRSMEEEGTIKSDWRIVAMTIDRCLLIFFALMVLITLFGCFSISPGYVP

Target ORF information:

RefSeq Version	XM_004208607.2
Organism	Hydra vulgaris
Definition	Hydra vulgaris neuronal acetylcholine receptor subunit alpha-2-like (LOC100215454), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004208607.2

ORF Insert Sequence:

ATGAAAATCA TTATTTTGAT TGTTACATAT TTATTCCAGT TTATAGAAAG TAGTCGTGAT 
GAAGAAAGAC TTTTAGATGA TTTGTTTAAA ACATATAATC CTGCTGCACG TCCAGTCTTG 
GATGATTCAG AATCAGTTAA TGTGACATTT GGGTTAACAT TACGACAAAT AATTGATGTG 
CATGAGAAAA TTCAAATACT TGTTATCAGT GCATGGATTA GACAGAAATG GAGAAATCCA 
ATCTTGACAT GGGATCCAAC AAAATATAAC AACATAAAAG AAATAAATGT AGATCCTTCA 
AGTATATGGC GGCCTGATAT TGTATTATAT AATAATGCAG ATGAAGATAA GACCTTTGGA 
GGTAATTTAG AAAGACTAAA AACAAGAGCT ATATTAAATT ATAATGGTGA CACTGCTTGG 
TTAGCTCCTG TCATTTTGAA GAGCAAATGT GATATTGATG TTAAGTATTT TCCTTTTGAT 
ACACAAAAGT GTCCTCTAAA ATTTGGCAGT TGGACATATG ACCAAGGAAG ATTAAATTTA 
ATTAACGAAA GCAATACAGC AACTTTAGAA AAATATTCAA ACAACTCTGA GTGGTATCTG 
CTAAGCGCAA AAGCAGTTCG AAATGAAGAA AAGTATTTCT GTTGTCCTGA ACTCTACCCT 
GATGTCACAT TTACGATTAT TATAGCTCGG CGATCTTTGT TTTACTTGTG TAATTTAATA 
TTCCCCATGA CAATAATTGG AATGTTAACT ATGTTATCAT TTTTATTGCC TGCCGAATCA 
GGCGAGAGAG TTTCACTTGC TATTACCCTT CTTCTTGCAA TGACTGTGTT CATGTTAGTT 
GTAGCTGATA TTATACCCGC TACAAGTGAT GTTATTCCAT TAGTTGGCAT TTATTTTAGT 
GTTTCAATGA TTGAAATGGT TATAATGATC ATTGTACTTT GTTACATCAT GAGGCTACAC 
AACAAGGGTC CAGGAGATCC GCCAATGCCT ATTTGGATGA GAAGATGGAT TTTGGAAAAC 
TTGTCGTACA AAGTTGGAAT TCGCGTACGA AGTGATCCGG ATCCTATGCA AATAAATAAT 
CAAAAGCTAA ATAACGACGA AAGTTCCATT CCTCTTAATA ATTTAAAGAT AAAGCAGTCC 
GAAATCAACT GGCAAAACAA AGTTTCAAAA TGTGAATTAT TACAATGCAA TGACAGCACT 
TTAAATAGTA CGCACAAAAA AAATAAGGCC ACTGTTCAAC TATCGGATTC AGAGCTTTTA 
TTGAGCAAAA TAGATGTTTT GCTGGAAAAG TTGCGTTCAA TGGAAGAAGA AGGAACAATA 
AAATCTGATT GGAGAATAGT AGCTATGACA ATTGATCGTT GCCTGCTGAT TTTTTTCGCA 
TTAATGGTGT TAATAACTCT ATTTGGTTGC TTCTCAATAT CACCAGGATA TGTTCCATAA