LINJ_36_2970 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_36_2970
Entrez Gene ID	5073560
Full Name	pre-mRNA splicing factor ATP-dependent RNA helicase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001469422.1	XP_001469459.1	pre-mRNA splicing factor ATP-dependent RNA helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_36_2970 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_36_2970 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc06763	XM_001469422.1 Latest version!	Leishmania infantum JPCM5 pre-mRNA splicing factor ATP-dependent RNA helicase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_36_2970 ( XM_001469422.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001469459.1 Leishmania infantum JPCM5 pre-mRNA splicing factor ATP-dependent RNA helicase partial mRNA.

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CloneID	OLc06763
Clone ID Related Accession (Same CDS sequence)	XM_001469422.1
Accession Version	XM_001469422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-15
Organism	Leishmania infantum JPCM5
Product	pre-mRNA splicing factor ATP-dependent RNA helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009420). COMPLETENESS: incomplete on both ends.

ATGAACGTTG TCGACGACAC TGTGGCGGCA CTGGGCGACG ACGACGCCAT CGCCGAGCAG 
CAGAACATGT CTTTCCTCAT GAAACTGCAG GAACTGTACT GCAAGACGGT TCTTTACGAG 
CAGCTGGAGC AGATGATGGG CCACCCCGCC ACCCAAGAAG AGAGCGACGG CTACCTTGAC 
GACGTGGAAG GGCTCGTCAA GCTCTACTGC TCCGAGAGGG AGGCGGCGAA GAAAATGGTG 
CCGCAAGGTG AGGAGGCCGA CGCCGCTGCT CAACGTGAGG AGGTGACGGA GCAGCACATC 
CTGCACTCTC TCTCCGTTGC TATCAACGGC GAGGCCGAGG CAGACGAGCA ACCCGACATA 
CCTGTGTCAA GCATTCAGCA GCTGTACCAC GTCCTCCACG ATTGCCTCAC CACGGACACG 
GTAGAGACAG GGCTCGCTGT CGGGTCATTG CTGCTGGCCG ATGATGCCAA ACGTAGCGCT 
GAGGAGGCGG CTCGTCTTGC CTATCAGCAG CAGCAGCGTC ACATGCACGG CGGCGTTGAC 
ACGGCCAGCA TGAACACCTC CACTGCGGAG CGCAAGAATG CCATGAAGGT CTCGCCAGAG 
GAGATGTTCC GCCTCGCCCA GCACGTCCGC AGCGGTCTTG CCGATGAAGC GGAGCTGCTC 
AAATTGCGCT CCGAGGACAA CGAGGAAGAA GGCGGCGGCG CGCAGGACGT GGAGCTGAAC 
GACGAAGAAC CTCGCTTCTT GCGCAACATG GGCTTCTCCT CGATGCTGCA CCGTCGCATC 
AACTACCGTG CACCGCTGCC GTCGCAGCAG CTCCGTGACG CCCGCACGCC GCAGCATCGT 
CAAGCCATCT TGGACAGGCT CGCCCAGCGT CAGCAGGGCG ACTCGTCAAA GCTGAACAGT 
ATGGAGGCGG TGGCGCAGAT GCAGGAGAAG ATAAATATGG AGCGCCGCGT CATTGCCCGC 
AAGGCGCAGC GTACCCAGCA GCAGTTCCGC GAGTACGACT TTGGGGCGAC CATGGGCGGT 
CGCGATGATA GGTCGGTGCT CGACGAGGAC CGCTACGGAC GGAGAGGCGG GGCGCTTGAA 
AGCGAGGAGT ACTCGGCACA GCCCACCCTT CTGCACTCGG AGCTGAACGC GCTGCCGGAG 
GAGGGCGGCT TCCGACCACA GGATATGCCG ACGAAGCTGG CGCCGTGGAT GAAACACAGC 
TTTGGACGCA AGCCGCGCTT TGGCCTGCCC GAGACTATGC AGACAATACA GGAGCAGCGC 
ATCTCCCTCC CGATTTACGC GAAGAAGGAG GCTCTTCTGA ACTTCGTAGA TGCGCACAGG 
GTGACCGTAC TTGTCGGAGA GACAGGCAGC GGCAAGACCA CGCAGATTCC GCAGTACCTG 
GCGGAGCACG GGTACGCCGA TCGAGGCATG ATCGCCTGCA CGCAGCCCCG CCGTGTCGCC 
GCCGAGACGC TTGCCATGCG CGTCGCTGAG GAGTACGGCT GCCGCCTTGG CGAGGAGGTC 
GGTTACACCG TTCGCTTCCG CGATGTCACC TCGTCTCTGA CAAAGATAAA GTACATGACG 
GACGGTATGC TGCTGCGCGA AGCGCTGCTG GACGACAGCT TCCAACGCTA CAGCGTCATC 
ATCCTCGACG AGGCTCACGA GCGATCCATC AGCACCGATC TGCTCTTCGC CATCGTGCGG 
CAGGCGCTGC GGAAGAACGA GGTGCTGAAG GTGATGGTGA CATCCGCCAC CCTCGAGACG 
GAGAAGTTCT GCGCCTACTT TGGCGCCTCT GAGCCTTTTC GGATTGAGGG TCGCACGTTC 
CCCGTGGAGA CGTACTACCT CACCGACCCC ACCACCGACT ACGTGCGAGC CGCCCTGCAG 
ACGGTCATGA TGATTCACCT GCAGGAGCCT CCAGGGGACG TCCTTGTCTT TTTCACTGGA 
CAGGAAGAGA TCGAGCTGGG CGGGGAGCAG CTGTTTCGCT GGATGGAGAT GCTGCGGCGG 
CAGGTGAGCA CGCCGTTGCC TGACTTGATG GTGCTGCCTC TCACGGCCAC CATGCCGCAG 
GAGGTGCAGT CGAAGGTGTT CGAGCCCACC CCACCCGGGT GCCGCAAGGT CGTACTGGCC 
ACGAACGTCG CAGAGACATC GATCACTATT GCCAACCTCT ACTACGTTGT TGACAGCGGC 
TTCTGCAAGC AGAACATCTT CGACGCCAAG CACGGCATCG ATCAGCTTAA GGTGATGCCG 
GTTTCTCAAG CGCAGGCGAA GCAGCGCTCC GGTCGTGCGG GGCGTATCGG GCCTGGAAAG 
TGCTACCGCA TGTACACGGA GAAGCAGTTC ACGACGGACA TGGTGCCGGA GACGGTGCCA 
GACATTATGC GGACAAGCCT CTTCCACGTT ACCCTGCAGC TGAAGGCCAT GGGGCTCGAT 
CTCCTTAACC TCGAGCTCAT GGACTGCCCT CCGAAGGGGG CCATTGTGTC AGCGCTAGAG 
AAGCTGCGAT ACCTTGAGGC GCTCGACGAC GACGGACTGC TGACCCCGCT AGGGAGCCGC 
ATGGCGCAGC TGTCCATCGA CCCCTCGCAG AGCAAAACGC TGCTCACTGC GGTGGACTTG 
GGTTGCAGCG AGCCGGTTCT CACAATTGTG TCGATGCTAG CAGTGCAGAA GCGTGGCGTC 
TTCTACCGCC CTCGAGATCA GCAAGATGCG TCTGACGCGG CGCGACGCCA GTTCATGCAA 
CCGGAGGGTG ATCAGCTCAC ACTCATGGCG GTCTATGACG CCTGGGTGGA GAACGGCATG 
TCCGAGGACT GGAGCAAGCA CAACTTCCTG AAGCACCGCA TGCTGGTCGA GGCGCGCGAC 
ACACGCGACC AGCTCAAGGA GATGCTGGCG CGGCGCAACC AGCACATATC ACACGAGAAT 
GACACAAACC TCGACGAGGT ACGCAAGTCC ATCACGGCCG GGTACTTCTT CAACGCGGCG 
AGGCGTGTCG ACTCGCACAC CCGCTCGTAC GTCACACTGT CCGACAGGCG TGAGGTTTAC 
GTGCACCCCT CCTCGGTGCT CATCGACGAC CCACCCAAGT ACGTTCTGTA CGACGATCTC 
CGCATGACGA AGCGGGAGTA CATGACGGAG CTGCTGGCGA TCGAGCCGAA GTGGTTGGTG 
GAGCTGGCGC CAGCCTTTTA CGCAAGACCG AAGGAAGGGC GTCTCACTAA GGAGCAAGCC 
GCTGAGCGGT TCACACCGAT CCTAAAGTCG TGGGAAACAG GCAGCTCCTG GCGTATCTCA 
CGTCTCAAGA AGCAGCGGCG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001469459.1
CDS	1..3264
Translation	MNVVDDTVAALGDDDAIAEQQNMSFLMKLQELYCKTVLYEQLEQMMGHPATQEESDGYLDDVEGLVKLYCSEREAAKKMVPQGEEADAAAQREEVTEQHILHSLSVAINGEAEADEQPDIPVSSIQQLYHVLHDCLTTDTVETGLAVGSLLLADDAKRSAEEAARLAYQQQQRHMHGGVDTASMNTSTAERKNAMKVSPEEMFRLAQHVRSGLADEAELLKLRSEDNEEEGGGAQDVELNDEEPRFLRNMGFSSMLHRRINYRAPLPSQQLRDARTPQHRQAILDRLAQRQQGDSSKLNSMEAVAQMQEKINMERRVIARKAQRTQQQFREYDFGATMGGRDDRSVLDEDRYGRRGGALESEEYSAQPTLLHSELNALPEEGGFRPQDMPTKLAPWMKHSFGRKPRFGLPETMQTIQEQRISLPIYAKKEALLNFVDAHRVTVLVGETGSGKTTQIPQYLAEHGYADRGMIACTQPRRVAAETLAMRVAEEYGCRLGEEVGYTVRFRDVTSSLTKIKYMTDGMLLREALLDDSFQRYSVIILDEAHERSISTDLLFAIVRQALRKNEVLKVMVTSATLETEKFCAYFGASEPFRIEGRTFPVETYYLTDPTTDYVRAALQTVMMIHLQEPPGDVLVFFTGQEEIELGGEQLFRWMEMLRRQVSTPLPDLMVLPLTATMPQEVQSKVFEPTPPGCRKVVLATNVAETSITIANLYYVVDSGFCKQNIFDAKHGIDQLKVMPVSQAQAKQRSGRAGRIGPGKCYRMYTEKQFTTDMVPETVPDIMRTSLFHVTLQLKAMGLDLLNLELMDCPPKGAIVSALEKLRYLEALDDDGLLTPLGSRMAQLSIDPSQSKTLLTAVDLGCSEPVLTIVSMLAVQKRGVFYRPRDQQDASDAARRQFMQPEGDQLTLMAVYDAWVENGMSEDWSKHNFLKHRMLVEARDTRDQLKEMLARRNQHISHENDTNLDEVRKSITAGYFFNAARRVDSHTRSYVTLSDRREVYVHPSSVLIDDPPKYVLYDDLRMTKREYMTELLAIEPKWLVELAPAFYARPKEGRLTKEQAAERFTPILKSWETGSSWRISRLKKQRR

Target ORF information:

RefSeq Version	XM_001469422.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 pre-mRNA splicing factor ATP-dependent RNA helicase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001469422.1

ORF Insert Sequence:

ATGAACGTTG TCGACGACAC TGTGGCGGCA CTGGGCGACG ACGACGCCAT CGCCGAGCAG 
CAGAACATGT CTTTCCTCAT GAAACTGCAG GAACTGTACT GCAAGACGGT TCTTTACGAG 
CAGCTGGAGC AGATGATGGG CCACCCCGCC ACCCAAGAAG AGAGCGACGG CTACCTTGAC 
GACGTGGAAG GGCTCGTCAA GCTCTACTGC TCCGAGAGGG AGGCGGCGAA GAAAATGGTG 
CCGCAAGGTG AGGAGGCCGA CGCCGCTGCT CAACGTGAGG AGGTGACGGA GCAGCACATC 
CTGCACTCTC TCTCCGTTGC TATCAACGGC GAGGCCGAGG CAGACGAGCA ACCCGACATA 
CCTGTGTCAA GCATTCAGCA GCTGTACCAC GTCCTCCACG ATTGCCTCAC CACGGACACG 
GTAGAGACAG GGCTCGCTGT CGGGTCATTG CTGCTGGCCG ATGATGCCAA ACGTAGCGCT 
GAGGAGGCGG CTCGTCTTGC CTATCAGCAG CAGCAGCGTC ACATGCACGG CGGCGTTGAC 
ACGGCCAGCA TGAACACCTC CACTGCGGAG CGCAAGAATG CCATGAAGGT CTCGCCAGAG 
GAGATGTTCC GCCTCGCCCA GCACGTCCGC AGCGGTCTTG CCGATGAAGC GGAGCTGCTC 
AAATTGCGCT CCGAGGACAA CGAGGAAGAA GGCGGCGGCG CGCAGGACGT GGAGCTGAAC 
GACGAAGAAC CTCGCTTCTT GCGCAACATG GGCTTCTCCT CGATGCTGCA CCGTCGCATC 
AACTACCGTG CACCGCTGCC GTCGCAGCAG CTCCGTGACG CCCGCACGCC GCAGCATCGT 
CAAGCCATCT TGGACAGGCT CGCCCAGCGT CAGCAGGGCG ACTCGTCAAA GCTGAACAGT 
ATGGAGGCGG TGGCGCAGAT GCAGGAGAAG ATAAATATGG AGCGCCGCGT CATTGCCCGC 
AAGGCGCAGC GTACCCAGCA GCAGTTCCGC GAGTACGACT TTGGGGCGAC CATGGGCGGT 
CGCGATGATA GGTCGGTGCT CGACGAGGAC CGCTACGGAC GGAGAGGCGG GGCGCTTGAA 
AGCGAGGAGT ACTCGGCACA GCCCACCCTT CTGCACTCGG AGCTGAACGC GCTGCCGGAG 
GAGGGCGGCT TCCGACCACA GGATATGCCG ACGAAGCTGG CGCCGTGGAT GAAACACAGC 
TTTGGACGCA AGCCGCGCTT TGGCCTGCCC GAGACTATGC AGACAATACA GGAGCAGCGC 
ATCTCCCTCC CGATTTACGC GAAGAAGGAG GCTCTTCTGA ACTTCGTAGA TGCGCACAGG 
GTGACCGTAC TTGTCGGAGA GACAGGCAGC GGCAAGACCA CGCAGATTCC GCAGTACCTG 
GCGGAGCACG GGTACGCCGA TCGAGGCATG ATCGCCTGCA CGCAGCCCCG CCGTGTCGCC 
GCCGAGACGC TTGCCATGCG CGTCGCTGAG GAGTACGGCT GCCGCCTTGG CGAGGAGGTC 
GGTTACACCG TTCGCTTCCG CGATGTCACC TCGTCTCTGA CAAAGATAAA GTACATGACG 
GACGGTATGC TGCTGCGCGA AGCGCTGCTG GACGACAGCT TCCAACGCTA CAGCGTCATC 
ATCCTCGACG AGGCTCACGA GCGATCCATC AGCACCGATC TGCTCTTCGC CATCGTGCGG 
CAGGCGCTGC GGAAGAACGA GGTGCTGAAG GTGATGGTGA CATCCGCCAC CCTCGAGACG 
GAGAAGTTCT GCGCCTACTT TGGCGCCTCT GAGCCTTTTC GGATTGAGGG TCGCACGTTC 
CCCGTGGAGA CGTACTACCT CACCGACCCC ACCACCGACT ACGTGCGAGC CGCCCTGCAG 
ACGGTCATGA TGATTCACCT GCAGGAGCCT CCAGGGGACG TCCTTGTCTT TTTCACTGGA 
CAGGAAGAGA TCGAGCTGGG CGGGGAGCAG CTGTTTCGCT GGATGGAGAT GCTGCGGCGG 
CAGGTGAGCA CGCCGTTGCC TGACTTGATG GTGCTGCCTC TCACGGCCAC CATGCCGCAG 
GAGGTGCAGT CGAAGGTGTT CGAGCCCACC CCACCCGGGT GCCGCAAGGT CGTACTGGCC 
ACGAACGTCG CAGAGACATC GATCACTATT GCCAACCTCT ACTACGTTGT TGACAGCGGC 
TTCTGCAAGC AGAACATCTT CGACGCCAAG CACGGCATCG ATCAGCTTAA GGTGATGCCG 
GTTTCTCAAG CGCAGGCGAA GCAGCGCTCC GGTCGTGCGG GGCGTATCGG GCCTGGAAAG 
TGCTACCGCA TGTACACGGA GAAGCAGTTC ACGACGGACA TGGTGCCGGA GACGGTGCCA 
GACATTATGC GGACAAGCCT CTTCCACGTT ACCCTGCAGC TGAAGGCCAT GGGGCTCGAT 
CTCCTTAACC TCGAGCTCAT GGACTGCCCT CCGAAGGGGG CCATTGTGTC AGCGCTAGAG 
AAGCTGCGAT ACCTTGAGGC GCTCGACGAC GACGGACTGC TGACCCCGCT AGGGAGCCGC 
ATGGCGCAGC TGTCCATCGA CCCCTCGCAG AGCAAAACGC TGCTCACTGC GGTGGACTTG 
GGTTGCAGCG AGCCGGTTCT CACAATTGTG TCGATGCTAG CAGTGCAGAA GCGTGGCGTC 
TTCTACCGCC CTCGAGATCA GCAAGATGCG TCTGACGCGG CGCGACGCCA GTTCATGCAA 
CCGGAGGGTG ATCAGCTCAC ACTCATGGCG GTCTATGACG CCTGGGTGGA GAACGGCATG 
TCCGAGGACT GGAGCAAGCA CAACTTCCTG AAGCACCGCA TGCTGGTCGA GGCGCGCGAC 
ACACGCGACC AGCTCAAGGA GATGCTGGCG CGGCGCAACC AGCACATATC ACACGAGAAT 
GACACAAACC TCGACGAGGT ACGCAAGTCC ATCACGGCCG GGTACTTCTT CAACGCGGCG 
AGGCGTGTCG ACTCGCACAC CCGCTCGTAC GTCACACTGT CCGACAGGCG TGAGGTTTAC 
GTGCACCCCT CCTCGGTGCT CATCGACGAC CCACCCAAGT ACGTTCTGTA CGACGATCTC 
CGCATGACGA AGCGGGAGTA CATGACGGAG CTGCTGGCGA TCGAGCCGAA GTGGTTGGTG 
GAGCTGGCGC CAGCCTTTTA CGCAAGACCG AAGGAAGGGC GTCTCACTAA GGAGCAAGCC 
GCTGAGCGGT TCACACCGAT CCTAAAGTCG TGGGAAACAG GCAGCTCCTG GCGTATCTCA 
CGTCTCAAGA AGCAGCGGCG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_36_2970 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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