| Gene Symbol |
GPX4 |
| Entrez Gene ID |
705333 |
| Full Name |
glutathione peroxidase 4 |
| Gene Type |
protein-coding |
| Organism |
Macaca mulatta(Rhesus monkey) |
| Summary |
The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016]. |
mRNA and Protein(s)
| mRNA |
Protein |
Name |
| NM_001118889.2 |
NP_001112361.1 |
phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A precursor |
| NM_001346420.1 |
NP_001333349.1 |
phospholipid hydroperoxide glutathione peroxidase isoform B |
| Homo sapiens (human) |
GPX4 |
NP_002076.2 |
| Bos taurus (cattle) |
GPX4 |
NP_777195.1 |
| Caenorhabditis elegans (roundworm) |
R03G5.5 |
NP_001123171.1 |
| Danio rerio (zebrafish) |
gpx4b |
NP_001025241.2 |
| Xenopus tropicalis (tropical clawed frog) |
gpx4 |
XP_002937424.2 |
|
GPX4 |
NP_001112361.1 |
| Saccharomyces cerevisiae (baker's yeast) |
GPX2 |
NP_009803.3 |
| Rattus norvegicus (Norway rat) |
Gpx4 |
NP_058861.3 |
| Arabidopsis thaliana (thale cress) |
GPX1 |
NP_180080.1 |
| Arabidopsis thaliana (thale cress) |
GPX7 |
NP_194915.2 |
| Mus musculus (house mouse) |
Gpx4 |
NP_001032830.2 |
| Caenorhabditis elegans (roundworm) |
T09A12.2 |
NP_001023368.1 |
Related articles in PubMed
A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Zimin AV, Cornish AS, Maudhoo MD, Gibbs RM, Zhang X, Pandey S, Meehan DT, Wipfler K, Bosinger SE, Johnson ZP, Tharp GK, Mar?ais G, Roberts M, Ferguson B, Fox HS, Treangen T, Salzberg SL, Yorke JA, Norgren RB Jr
Biology direct9(1)20(2014 Oct)
Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)
Analysis of the Macaca mulatta transcriptome and the sequence divergence between Macaca and human.
Magness CL, Fellin PC, Thomas MJ, Korth MJ, Agy MB, Proll SC, Fitzgibbon M, Scherer CA, Miner DG, Katze MG, Iadonato SP
Genome biology6(7)R60(2005)
Characterization of mammalian selenoproteomes.
Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guig? R, Gladyshev VN
Science (New York, N.Y.)300(5624)1439-43(2003 May)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The following GPX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID |
OMb02727 |
| Clone ID Related Accession (Same CDS sequence) |
NM_001118889.2
|
| Accession Version |
NM_001118889.2 Latest version! |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
 User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2019-04-27 |
| Organism |
Macaca mulatta(Rhesus monkey) |
| Product |
phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A precursor |
| Comment |
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from JV649198.1, JV681449.1 and JV465885.1. On Oct 11, 2016 this sequence version replaced NM_001118889.1.
Transcript Variant: This variant (1) represents the predominant transcript and encodes the canonical isoform A. A similar isoform in rat has been shown to be localized to the mitochondria (PMID:9988735).
##Evidence-Data-START##
Transcript exon combination :: JV649198.1, JV681449.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA4688315, SAMEA4688316 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
gene product(s) localized to mito. :: inferred from homology
protein contains selenocysteine :: inferred from conservation
##RefSeq-Attributes-END##
COMPLETENESS: complete on the 3' end.
|
1
61
121
181
241
301
361
421
481
541
| ATGAACCTCG GCCGCCTTTG CCGCCTGCTG AAGCCGGCGC TGCTCTGTGG GGCTCTGGCC
GCGCCCGGCC TCGCCGGTAC CATGTGCGCG TCCCGGGACG ACTGGCGCTG TGCGCGCTCC
ATGCACGAGT TTTCCGCGAA AGACATCGAC GGGCACATGG TTAACCTGGA CAAGTACCGG
GGGTTCGTGT GCATCGTCAC CAACGTGGCC TCCCAGTGAG GCAAGACCGA AGTGAACTAC
ACTCAGCTCG TCGACCTGCA CGCCCGATAC GCTGAGTGCG GTTTGCGGAT CCTGGCCTTC
CCATGTAACC AGTTCGGGAA GCAGGAGCCA GGGAGTAACG AAGAGATCAA AGAGTTCGCC
GCAGGCTACA ACGTCAAATT CGATATGTTC AGCAAGATAT GCGTGAACGG GGACGATGCC
CACCCGCTGT GGAAGTGGAT GAAGATCCAG CCCAAGGGCA AGGGCATCCT GGGAAATGCC
ATCAAGTGGA ACTTCACCAA GTTCCTCATT GACAAGAACG GCTGCGTGGT GAAGCGGTAC
GGACCCATGG AGGAACCCCT GGTGATAGAG AAGGACCTGC CCCACTATTT CTAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
NP_001112361.1 |
| CDS | 73..666 |
| Translation | |
Target ORF information:
| RefSeq Version | NM_001118889.2 |
| Organism | Macaca mulatta(Rhesus monkey) |
| Definition | Macaca mulatta glutathione peroxidase 4 (GPX4), transcript variant 1, mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
 NM_001118889.2 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
| ATGAACCTCG GCCGCCTTTG CCGCCTGCTG AAGCCGGCGC TGCTCTGTGG GGCTCTGGCC
GCGCCCGGCC TCGCCGGTAC CATGTGCGCG TCCCGGGACG ACTGGCGCTG TGCGCGCTCC
ATGCACGAGT TTTCCGCGAA AGACATCGAC GGGCACATGG TTAACCTGGA CAAGTACCGG
GGGTTCGTGT GCATCGTCAC CAACGTGGCC TCCCAGTGAG GCAAGACCGA AGTGAACTAC
ACTCAGCTCG TCGACCTGCA CGCCCGATAC GCTGAGTGCG GTTTGCGGAT CCTGGCCTTC
CCATGTAACC AGTTCGGGAA GCAGGAGCCA GGGAGTAACG AAGAGATCAA AGAGTTCGCC
GCAGGCTACA ACGTCAAATT CGATATGTTC AGCAAGATAT GCGTGAACGG GGACGATGCC
CACCCGCTGT GGAAGTGGAT GAAGATCCAG CCCAAGGGCA AGGGCATCCT GGGAAATGCC
ATCAAGTGGA ACTTCACCAA GTTCCTCATT GACAAGAACG GCTGCGTGGT GAAGCGGTAC
GGACCCATGG AGGAACCCCT GGTGATAGAG AAGGACCTGC CCCACTATTT CTAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID |
OMb57578 |
| Clone ID Related Accession (Same CDS sequence) |
NM_001346420.1
|
| Accession Version |
NM_001346420.1 Latest version! |
Documents for ORF clone product in default vector |
| Sequence Information |
ORF Nucleotide Sequence (Length: 705bp)
Protein sequence
SNP |
| Vector |
pcDNA3.1+/C-(K)DYK or customized vector |
User Manual |
| Clone information |
Clone Map |
MSDS |
| Tag on pcDNA3.1+/C-(K)DYK |
C terminal DYKDDDDK tags |
| ORF Insert Method |
CloneEZ™ Seamless cloning technology |
| Insert Structure |
linear |
| Update Date |
2019-04-27 |
| Organism |
Macaca mulatta(Rhesus monkey) |
| Product |
phospholipid hydroperoxide glutathione peroxidase isoform B |
| Comment |
Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from JV602446.1 and JV465885.1.
Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. The resulting isoform (B) has a longer and a distinct N-terminus compared to isoform A.
##Evidence-Data-START##
Transcript exon combination :: JV602446.1, JV667383.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns SAMEA4688316, SAMEA4688317 [ECO:0000348]
##Evidence-Data-END##
##RefSeq-Attributes-START##
gene product(s) localized to mito. :: inferred from homology
protein contains selenocysteine :: inferred from conservation
##RefSeq-Attributes-END##
COMPLETENESS: complete on the 3' end.
|
1
61
121
181
241
301
361
421
481
541
601
661
| ATGGGCCGCG CGGGCGCAGG CTCCCCCAGG CGCCGCAGGC AGCGGTGCCA GAGCCGGGGC
AGACGGCGGC CGCGAGCCCC TCGACGGCGG AAGGCCCCAG CGTGCAGGCG CAGGAGGGCG
CGGCGCCGGC GGAAGGAGCC CTGTCCCCGC AGCTTGGGAC CGGAGATCCA CGAATGTCCC
GAATCCCAGG ACCCGTGCGC GTCCCGGGAC GACTGGCGCT GTGCGCGCTC CATGCACGAG
TTTTCCGCGA AAGACATCGA CGGGCACATG GTTAACCTGG ACAAGTACCG GGGGTTCGTG
TGCATCGTCA CCAACGTGGC CTCCCAGTGA GGCAAGACCG AAGTGAACTA CACTCAGCTC
GTCGACCTGC ACGCCCGATA CGCTGAGTGC GGTTTGCGGA TCCTGGCCTT CCCATGTAAC
CAGTTCGGGA AGCAGGAGCC AGGGAGTAAC GAAGAGATCA AAGAGTTCGC CGCAGGCTAC
AACGTCAAAT TCGATATGTT CAGCAAGATA TGCGTGAACG GGGACGATGC CCACCCGCTG
TGGAAGTGGA TGAAGATCCA GCCCAAGGGC AAGGGCATCC TGGGAAATGC CATCAAGTGG
AACTTCACCA AGTTCCTCAT TGACAAGAAC GGCTGCGTGG TGAAGCGGTA CGGACCCATG
GAGGAACCCC TGGTGATAGA GAAGGACCTG CCCCACTATT TCTAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq |
NP_001333349.1 |
| CDS | 90..794 |
| Translation | |
Target ORF information:
| RefSeq Version | NM_001346420.1 |
| Organism | Macaca mulatta(Rhesus monkey) |
| Definition | Macaca mulatta glutathione peroxidase 4 (GPX4), transcript variant 2, mRNA. |
Target ORF information:
| Epitope | DYKDDDDK |
| Bacterial selection | AMPR |
| Mammalian selection | NeoR |
| Vector | pcDNA3.1+/C-(K)DYK |
|
NM_001346420.1 |
ORF Insert Sequence:
1
61
121
181
241
301
361
421
481
541
601
661
| ATGGGCCGCG CGGGCGCAGG CTCCCCCAGG CGCCGCAGGC AGCGGTGCCA GAGCCGGGGC
AGACGGCGGC CGCGAGCCCC TCGACGGCGG AAGGCCCCAG CGTGCAGGCG CAGGAGGGCG
CGGCGCCGGC GGAAGGAGCC CTGTCCCCGC AGCTTGGGAC CGGAGATCCA CGAATGTCCC
GAATCCCAGG ACCCGTGCGC GTCCCGGGAC GACTGGCGCT GTGCGCGCTC CATGCACGAG
TTTTCCGCGA AAGACATCGA CGGGCACATG GTTAACCTGG ACAAGTACCG GGGGTTCGTG
TGCATCGTCA CCAACGTGGC CTCCCAGTGA GGCAAGACCG AAGTGAACTA CACTCAGCTC
GTCGACCTGC ACGCCCGATA CGCTGAGTGC GGTTTGCGGA TCCTGGCCTT CCCATGTAAC
CAGTTCGGGA AGCAGGAGCC AGGGAGTAAC GAAGAGATCA AAGAGTTCGC CGCAGGCTAC
AACGTCAAAT TCGATATGTT CAGCAAGATA TGCGTGAACG GGGACGATGC CCACCCGCTG
TGGAAGTGGA TGAAGATCCA GCCCAAGGGC AAGGGCATCC TGGGAAATGC CATCAAGTGG
AACTTCACCA AGTTCCTCAT TGACAAGAAC GGCTGCGTGG TGAAGCGGTA CGGACCCATG
GAGGAACCCC TGGTGATAGA GAAGGACCTG CCCCACTATT TCTAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
A new rhesus macaque assembly and annotation for next-generation sequencing analyses.
Biology direct9(1)20(2014 Oct)
Zimin AV,Cornish AS,Maudhoo MD,Gibbs RM,Zhang X,Pandey S,Meehan DT,Wipfler K,Bosinger SE,Johnson ZP,Tharp GK,Mar?ais G,Roberts M,Ferguson B,Fox HS,Treangen T,Salzberg SL,Yorke JA,Norgren RB Jr |
|
Analysis of the Macaca mulatta transcriptome and the sequence divergence between Macaca and human.
Genome biology6(7)R60(2005)
Magness CL,Fellin PC,Thomas MJ,Korth MJ,Agy MB,Proll SC,Fitzgibbon M,Scherer CA,Miner DG,Katze MG,Iadonato SP |
