gpx4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)
| Gene Symbol | gpx4 |
| Entrez Gene ID | 100496541 |
| Full Name | glutathione peroxidase 4 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Xenopus tropicalis(tropical clawed frog) |
| Summary | The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development. Disruption of this gene in mouse spermatocytes is associated with male infertility. This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016]. |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_001346486.1 | NP_001333415.1 | phospholipid hydroperoxide glutathione peroxidase isoform B |
| NM_001304772.1 | NP_001291701.1 | phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A |
| Homo sapiens (human) | GPX4 | NP_002076.2 |
| Bos taurus (cattle) | GPX4 | NP_777195.1 |
| Caenorhabditis elegans (roundworm) | R03G5.5 | NP_001123171.1 |
| Danio rerio (zebrafish) | gpx4b | NP_001025241.2 |
| Xenopus tropicalis (tropical clawed frog) | gpx4 | XP_002937424.2 |
| GPX4 | NP_001112361.1 | |
| Saccharomyces cerevisiae (baker's yeast) | GPX2 | NP_009803.3 |
| Rattus norvegicus (Norway rat) | Gpx4 | NP_058861.3 |
| Arabidopsis thaliana (thale cress) | GPX1 | NP_180080.1 |
| Arabidopsis thaliana (thale cress) | GPX7 | NP_194915.2 |
| Mus musculus (house mouse) | Gpx4 | NP_001032830.2 |
| Caenorhabditis elegans (roundworm) | T09A12.2 | NP_001023368.1 |
Related articles in PubMed
Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The following gpx4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpx4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OXa04331 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001304772.1 | |
| Accession Version | NM_001304772.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-21 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | phospholipid hydroperoxide glutathione peroxidase, mitochondrial isoform A | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EL714502.1 and DN024015.1. On Feb 9, 2015 this sequence version replaced XM_002937378.2. Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform A. A similar isoform in rat has been shown to be localized to the mitochondria (PMID:9988735). ##Evidence-Data-START## Transcript exon combination :: EL714502.1, CX430339.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTTGAATT TGCTGTGTCG CAGCATCAAG CGCTCAGTGC TGCTTGGATC TGTGATCGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001291701.1 |
| CDS | 23..613 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001304772.1 |
ORF Insert Sequence:
1 | ATGTTGAATT TGCTGTGTCG CAGCATCAAG CGCTCAGTGC TGCTTGGATC TGTGATCGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OXa47199 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001346486.1 | |
| Accession Version | NM_001346486.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-21 | |
| Organism | Xenopus tropicalis(tropical clawed frog) | |
| Product | phospholipid hydroperoxide glutathione peroxidase isoform B | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EL714502.1, EL849356.1 and DN024015.1. On Oct 14, 2016 this sequence version replaced NR_130901.1. Transcript Variant: This variant (2) contains an additional in-frame exon in the 5' coding region, and uses an alternate translation start codon compared to variant 1. The resulting isoform (B) has a distinct N-terminus compared to isoform A. ##Evidence-Data-START## Transcript exon combination :: DN027724.1, DN027725.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGCACAGAG GATTAGCTGA ATTCAGGAAT GGATGCCCTT TATCTGGAAT GGATCCCTCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001333415.1 |
| CDS | 127..714 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001346486.1 |
ORF Insert Sequence:
1 | ATGCACAGAG GATTAGCTGA ATTCAGGAAT GGATGCCCTT TATCTGGAAT GGATCCCTCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Selenoprotein Gene Nomenclature. |
|
Composition and evolution of the vertebrate and mammalian selenoproteomes. |