ORF » Species Summary » Megachile rotundata » LOC100876415 cDNA ORF clone

LOC100876415 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

The following LOC100876415 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100876415 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe115268 XM_012297427.1
Latest version!		 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OMe115269 XM_012297428.1
Latest version!		 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OMe115269 XM_003708367.2
Latest version!		 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OMe115270 XM_012297429.1
Latest version!		 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OMe115271 XM_012297433.1
Latest version!		 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OMe115271 XM_012297430.1
Latest version!		 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OMe115272 XM_012297431.1
Latest version!		 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC100876415 ( XM_012297427.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012152817.1 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OMe115268
    Clone ID Related Accession (Same CDS sequence) XM_012297427.1
    Accession Version XM_012297427.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product apoptosis-resistant E3 ubiquitin protein ligase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003798699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCACGAT GGTCGACTGT ATTGTCGGGG GTATTCCTGG CCCTTTCTAT GGTCCTGTCG 
    CTTGGCAAAC TTCTGATGCT TGCCACCGGA AATGGAAACA ACGGGGATCT CACCGAAGCG 
    GATCAGTGGC TCGCTGAAAT TGGCCTAAAA CAATACCGGC CTCTCTTCAA AAAGAAAGGC 
    ATTTCAACCC TTGCAAGTTG CGGCGAAGTG GACGCGTTGC CAGAACTCCC GGCACAGGAC 
    GAGGAACGAT TGCAACGCGC AGCGCGTTTG CTGCAACAGA GACTCGTTTT ACGTCAGTGG 
    TTGACGGACC ATGGCCTGCA TAGCCACTAC CAAAAGCTGA TGCAGATGGA AGTGATGTCT 
    CTCGAGGACG TGTACTGGGT AGAAGAGAAC ACGGTACGGG CAGCTTTTGG CAAAGATCTA 
    CAACGGTGGA ACGAGGCCAG ACAGACATTG CCTATTTCGA AGGAAGATTT AGAAACCCTC 
    AAGGCTGATC TTTGGAGCGC CGTTGTCAAG AACAGCCAAC ATCAAGACGC CTGGACATGG 
    GGTGGAATGT TAGTAGTTTC CGTGTCGGTA GCAGGGCTGG TGACCCTAGC TGCTATGACA 
    CAGCCAGCCC TAGCACCAGA AGCAAAACAC TCCCTCCTGC AGTACGTAAC CGGAAAGTAT 
    TTGTTACCAA GCAACTGCCG CGTTCACTTC GAATGGGACG AGCCACAGCT CGTAGGGGAG 
    ACAATGACTT TTACCGTGAA GTTTTATCAA CGCAACGGCC AACCGTATCC GATCTGTGAC 
    AAGGACAATT TAATCGTAGA AGTCACAGAA GGCACGCGAA GGGTGGCGAC CGTAATTGAG 
    TTGGGAGGCA CCGACCCGTT AGCTGCTAAC ACCGCAGTAG TCAAGTTTAC TGTCCGCCAT 
    GCTGGACAAT ATCGAATAGC CGTACTTATT GGTTCGTGTC ACGTTCACGG AAGCCCGTTT 
    CTTAAGAATT TTCTTCCTGG TCCTCCAGAT CCAAATAAGA CAGTCTTCGT ACGGCAAAGT 
    TCTACGGTAG TTTGTACAGC TGGAATTGCA CACTCTATGA CAATAGAGCC ACGAGACGAA 
    TACGATAATT TGTGCGTATT TGGACCTGGA GATAATCCTA CGGAAGGATA CCAAGTCAAT 
    ATTACTCAGA TTGGGAATGT AGTAGATAAA TCGGCGGTTA CGAATTGTAG TATACAGCTT 
    GATTACGACT CTCCGAATCA AAGGATTCGG TTGAAAGTTA GCTTCCCAAA GGGTGGTTGT 
    TATCACGCGA CAGTCAGTTT AGCTGGACTG CAATTGCACA ATGGGGATTT CGACATCATT 
    GTACTCGAAA GTGATGTTGC AAGAATGGTA CACAACAACG TTGCTTCGAA GGACCCCGAC 
    ATTTGTTACG CTGCAAAATT GTTAGGCATG CAAGGCGAGA AATTTTCAAA GCCGAAAAAA 
    GTTTTCTGTT TTATTTCGCC TAAGCAGCTC ACCATCAAGG AATATTTGTT GAGAATAATA 
    CCGAAGAGAC TCGTTACTTT CAGGCTTTGT CCTTCTACCA AATTTCATTT TGATTGTTCG 
    AATAATCAAT ACGATGGTTA CGACTCTTTC TCTATCGACG ATGGATGTCA GCCACCCGTC 
    GAATTGATTT CTCTGTACAG AGATATAATA GCCGCTACAT TTACGTTGTT CCTTTTGAAG 
    AACATAGGTG GAAGCGAAAC TTTTGCCGAT AAACAAGATT TCTTTTATCA TGAAGTTCGA 
    AAACATCATC AGAAACATTA TCACGAAAAA CTTTCGATGA AAGTTCAAAG GGACAAATTA 
    CTGGAATCGT CTATGAAAGC CACCAAAGGA TTTTCCGTGA GCGACTGGTG CAGAAATTTC 
    GAAATCACTT TCCAAGGTGA ACAAGGCGTC GATTGGGGAG GCGTTAGACG AGAATGGTTC 
    GAACTAATTT GCGCGGCTCT ATTCGATTCA GGAAACGGCT TATTCGCTTC CTTTGGAGAA 
    TCCCAGCAAG CTTTGGTACA TCCCAATAGT AAACGGCCTC AACACCTCAA GTTAAAGCAC 
    TATGAGTTTG CAGGTCGAAT CGTGGGCAAA TGCCTGTATG AATCTGCACT TGGTGGTTCC 
    TATCGCCAAT TAGTTCGTGC AAGATTTACA AGATCATTTC TTGCGCAAAT CATAGGCCTC 
    AGGGTACACT ACAAGTATTT CGAGCAGGAT GACCCAGACC TATACCTAAG CAAGATAAAA 
    TATATTCTTG AGAACGACGT GGAGGAAATG GAATTGTATT TTGTTGAAGA AGAGTACGAT 
    AAAGATGGTC AATTATTAAA AGTAGCCGAG TTAATACCAG GAGGCAGTAA AATTCGTGTC 
    ACGAACGAAA CGAAACTGCG TTATTTGGAT GCATTGGCAC AACACAGACT AGCCAGTTCT 
    ATACGAAACG AGGTAGAACA TTTTCTAAGG GGCCTGAACG AGCTCATCCC CGACAACCTT 
    TTAGGAATAT TCGATGAAAA TGAACTAGAA TTGTTACTAT GTGGAACTGG AGAATACAGC 
    GTGGCAGATC TACGAGCGCA CCATATAGCT AATGGGAGTT CTCCGGAGTT TCTCCGAGTT 
    CTGGATTGGT TCTGGACAGC AGTTAGTAAT TTCACACAGG AGGAGATGGC CCGATTACTA 
    CAATTCACTA CAGGTTGCTC GCAATTACCA CCCGGTGGAT TCCAACAGCT AAATCCACGA 
    TTTCAGATCA CAGCAGCACC AACATTTGCT AATTTGCCTA CAGCACATAC TTGCTTCAAT 
    CAACTCTGCT TACCAGATTA CGAGTGTTAC GATCACTTCG AACGTGCTTT ATTATTAGCG 
    ATTAGCGAGG GAACCGAAGG TTTTGGTATG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012152817.1
    CDS184..3039
    Translation

    Target ORF information:

    RefSeq Version XM_012297427.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012297427.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCACGAT GGTCGACTGT ATTGTCGGGG GTATTCCTGG CCCTTTCTAT GGTCCTGTCG 
    CTTGGCAAAC TTCTGATGCT TGCCACCGGA AATGGAAACA ACGGGGATCT CACCGAAGCG 
    GATCAGTGGC TCGCTGAAAT TGGCCTAAAA CAATACCGGC CTCTCTTCAA AAAGAAAGGC 
    ATTTCAACCC TTGCAAGTTG CGGCGAAGTG GACGCGTTGC CAGAACTCCC GGCACAGGAC 
    GAGGAACGAT TGCAACGCGC AGCGCGTTTG CTGCAACAGA GACTCGTTTT ACGTCAGTGG 
    TTGACGGACC ATGGCCTGCA TAGCCACTAC CAAAAGCTGA TGCAGATGGA AGTGATGTCT 
    CTCGAGGACG TGTACTGGGT AGAAGAGAAC ACGGTACGGG CAGCTTTTGG CAAAGATCTA 
    CAACGGTGGA ACGAGGCCAG ACAGACATTG CCTATTTCGA AGGAAGATTT AGAAACCCTC 
    AAGGCTGATC TTTGGAGCGC CGTTGTCAAG AACAGCCAAC ATCAAGACGC CTGGACATGG 
    GGTGGAATGT TAGTAGTTTC CGTGTCGGTA GCAGGGCTGG TGACCCTAGC TGCTATGACA 
    CAGCCAGCCC TAGCACCAGA AGCAAAACAC TCCCTCCTGC AGTACGTAAC CGGAAAGTAT 
    TTGTTACCAA GCAACTGCCG CGTTCACTTC GAATGGGACG AGCCACAGCT CGTAGGGGAG 
    ACAATGACTT TTACCGTGAA GTTTTATCAA CGCAACGGCC AACCGTATCC GATCTGTGAC 
    AAGGACAATT TAATCGTAGA AGTCACAGAA GGCACGCGAA GGGTGGCGAC CGTAATTGAG 
    TTGGGAGGCA CCGACCCGTT AGCTGCTAAC ACCGCAGTAG TCAAGTTTAC TGTCCGCCAT 
    GCTGGACAAT ATCGAATAGC CGTACTTATT GGTTCGTGTC ACGTTCACGG AAGCCCGTTT 
    CTTAAGAATT TTCTTCCTGG TCCTCCAGAT CCAAATAAGA CAGTCTTCGT ACGGCAAAGT 
    TCTACGGTAG TTTGTACAGC TGGAATTGCA CACTCTATGA CAATAGAGCC ACGAGACGAA 
    TACGATAATT TGTGCGTATT TGGACCTGGA GATAATCCTA CGGAAGGATA CCAAGTCAAT 
    ATTACTCAGA TTGGGAATGT AGTAGATAAA TCGGCGGTTA CGAATTGTAG TATACAGCTT 
    GATTACGACT CTCCGAATCA AAGGATTCGG TTGAAAGTTA GCTTCCCAAA GGGTGGTTGT 
    TATCACGCGA CAGTCAGTTT AGCTGGACTG CAATTGCACA ATGGGGATTT CGACATCATT 
    GTACTCGAAA GTGATGTTGC AAGAATGGTA CACAACAACG TTGCTTCGAA GGACCCCGAC 
    ATTTGTTACG CTGCAAAATT GTTAGGCATG CAAGGCGAGA AATTTTCAAA GCCGAAAAAA 
    GTTTTCTGTT TTATTTCGCC TAAGCAGCTC ACCATCAAGG AATATTTGTT GAGAATAATA 
    CCGAAGAGAC TCGTTACTTT CAGGCTTTGT CCTTCTACCA AATTTCATTT TGATTGTTCG 
    AATAATCAAT ACGATGGTTA CGACTCTTTC TCTATCGACG ATGGATGTCA GCCACCCGTC 
    GAATTGATTT CTCTGTACAG AGATATAATA GCCGCTACAT TTACGTTGTT CCTTTTGAAG 
    AACATAGGTG GAAGCGAAAC TTTTGCCGAT AAACAAGATT TCTTTTATCA TGAAGTTCGA 
    AAACATCATC AGAAACATTA TCACGAAAAA CTTTCGATGA AAGTTCAAAG GGACAAATTA 
    CTGGAATCGT CTATGAAAGC CACCAAAGGA TTTTCCGTGA GCGACTGGTG CAGAAATTTC 
    GAAATCACTT TCCAAGGTGA ACAAGGCGTC GATTGGGGAG GCGTTAGACG AGAATGGTTC 
    GAACTAATTT GCGCGGCTCT ATTCGATTCA GGAAACGGCT TATTCGCTTC CTTTGGAGAA 
    TCCCAGCAAG CTTTGGTACA TCCCAATAGT AAACGGCCTC AACACCTCAA GTTAAAGCAC 
    TATGAGTTTG CAGGTCGAAT CGTGGGCAAA TGCCTGTATG AATCTGCACT TGGTGGTTCC 
    TATCGCCAAT TAGTTCGTGC AAGATTTACA AGATCATTTC TTGCGCAAAT CATAGGCCTC 
    AGGGTACACT ACAAGTATTT CGAGCAGGAT GACCCAGACC TATACCTAAG CAAGATAAAA 
    TATATTCTTG AGAACGACGT GGAGGAAATG GAATTGTATT TTGTTGAAGA AGAGTACGAT 
    AAAGATGGTC AATTATTAAA AGTAGCCGAG TTAATACCAG GAGGCAGTAA AATTCGTGTC 
    ACGAACGAAA CGAAACTGCG TTATTTGGAT GCATTGGCAC AACACAGACT AGCCAGTTCT 
    ATACGAAACG AGGTAGAACA TTTTCTAAGG GGCCTGAACG AGCTCATCCC CGACAACCTT 
    TTAGGAATAT TCGATGAAAA TGAACTAGAA TTGTTACTAT GTGGAACTGG AGAATACAGC 
    GTGGCAGATC TACGAGCGCA CCATATAGCT AATGGGAGTT CTCCGGAGTT TCTCCGAGTT 
    CTGGATTGGT TCTGGACAGC AGTTAGTAAT TTCACACAGG AGGAGATGGC CCGATTACTA 
    CAATTCACTA CAGGTTGCTC GCAATTACCA CCCGGTGGAT TCCAACAGCT AAATCCACGA 
    TTTCAGATCA CAGCAGCACC AACATTTGCT AATTTGCCTA CAGCACATAC TTGCTTCAAT 
    CAACTCTGCT TACCAGATTA CGAGTGTTAC GATCACTTCG AACGTGCTTT ATTATTAGCG 
    ATTAGCGAGG GAACCGAAGG TTTTGGTATG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100876415 ( XM_003708367.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003708415.1 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OMe115269
    Clone ID Related Accession (Same CDS sequence) XM_003708367.2 , XM_012297428.1
    Accession Version XM_003708367.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product apoptosis-resistant E3 ubiquitin protein ligase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003798699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003708367.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCGGACA AAATTCGAGG CTGTCGAGAT GGACAACGCG ACAAAGATTT CTTTGCCGAC 
    GAGATATCGT CCATCGGCAT GTCGCTGCGG CCCAGCGGTA GCGCTTCTTC CGGCATTTCA 
    ACCCTTGCAA GTTGCGGCGA AGTGGACGCG TTGCCAGAAC TCCCGGCACA GGACGAGGAA 
    CGATTGCAAC GCGCAGCGCG TTTGCTGCAA CAGAGACTCG TTTTACGTCA GTGGTTGACG 
    GACCATGGCC TGCATAGCCA CTACCAAAAG CTGATGCAGA TGGAAGTGAT GTCTCTCGAG 
    GACGTGTACT GGGTAGAAGA GAACACGGTA CGGGCAGCTT TTGGCAAAGA TCTACAACGG 
    TGGAACGAGG CCAGACAGAC ATTGCCTATT TCGAAGGAAG ATTTAGAAAC CCTCAAGGCT 
    GATCTTTGGA GCGCCGTTGT CAAGAACAGC CAACATCAAG ACGCCTGGAC ATGGGGTGGA 
    ATGTTAGTAG TTTCCGTGTC GGTAGCAGGG CTGGTGACCC TAGCTGCTAT GACACAGCCA 
    GCCCTAGCAC CAGAAGCAAA ACACTCCCTC CTGCAGTACG TAACCGGAAA GTATTTGTTA 
    CCAAGCAACT GCCGCGTTCA CTTCGAATGG GACGAGCCAC AGCTCGTAGG GGAGACAATG 
    ACTTTTACCG TGAAGTTTTA TCAACGCAAC GGCCAACCGT ATCCGATCTG TGACAAGGAC 
    AATTTAATCG TAGAAGTCAC AGAAGGCACG CGAAGGGTGG CGACCGTAAT TGAGTTGGGA 
    GGCACCGACC CGTTAGCTGC TAACACCGCA GTAGTCAAGT TTACTGTCCG CCATGCTGGA 
    CAATATCGAA TAGCCGTACT TATTGGTTCG TGTCACGTTC ACGGAAGCCC GTTTCTTAAG 
    AATTTTCTTC CTGGTCCTCC AGATCCAAAT AAGACAGTCT TCGTACGGCA AAGTTCTACG 
    GTAGTTTGTA CAGCTGGAAT TGCACACTCT ATGACAATAG AGCCACGAGA CGAATACGAT 
    AATTTGTGCG TATTTGGACC TGGAGATAAT CCTACGGAAG GATACCAAGT CAATATTACT 
    CAGATTGGGA ATGTAGTAGA TAAATCGGCG GTTACGAATT GTAGTATACA GCTTGATTAC 
    GACTCTCCGA ATCAAAGGAT TCGGTTGAAA GTTAGCTTCC CAAAGGGTGG TTGTTATCAC 
    GCGACAGTCA GTTTAGCTGG ACTGCAATTG CACAATGGGG ATTTCGACAT CATTGTACTC 
    GAAAGTGATG TTGCAAGAAT GGTACACAAC AACGTTGCTT CGAAGGACCC CGACATTTGT 
    TACGCTGCAA AATTGTTAGG CATGCAAGGC GAGAAATTTT CAAAGCCGAA AAAAGTTTTC 
    TGTTTTATTT CGCCTAAGCA GCTCACCATC AAGGAATATT TGTTGAGAAT AATACCGAAG 
    AGACTCGTTA CTTTCAGGCT TTGTCCTTCT ACCAAATTTC ATTTTGATTG TTCGAATAAT 
    CAATACGATG GTTACGACTC TTTCTCTATC GACGATGGAT GTCAGCCACC CGTCGAATTG 
    ATTTCTCTGT ACAGAGATAT AATAGCCGCT ACATTTACGT TGTTCCTTTT GAAGAACATA 
    GGTGGAAGCG AAACTTTTGC CGATAAACAA GATTTCTTTT ATCATGAAGT TCGAAAACAT 
    CATCAGAAAC ATTATCACGA AAAACTTTCG ATGAAAGTTC AAAGGGACAA ATTACTGGAA 
    TCGTCTATGA AAGCCACCAA AGGATTTTCC GTGAGCGACT GGTGCAGAAA TTTCGAAATC 
    ACTTTCCAAG GTGAACAAGG CGTCGATTGG GGAGGCGTTA GACGAGAATG GTTCGAACTA 
    ATTTGCGCGG CTCTATTCGA TTCAGGAAAC GGCTTATTCG CTTCCTTTGG AGAATCCCAG 
    CAAGCTTTGG TACATCCCAA TAGTAAACGG CCTCAACACC TCAAGTTAAA GCACTATGAG 
    TTTGCAGGTC GAATCGTGGG CAAATGCCTG TATGAATCTG CACTTGGTGG TTCCTATCGC 
    CAATTAGTTC GTGCAAGATT TACAAGATCA TTTCTTGCGC AAATCATAGG CCTCAGGGTA 
    CACTACAAGT ATTTCGAGCA GGATGACCCA GACCTATACC TAAGCAAGAT AAAATATATT 
    CTTGAGAACG ACGTGGAGGA AATGGAATTG TATTTTGTTG AAGAAGAGTA CGATAAAGAT 
    GGTCAATTAT TAAAAGTAGC CGAGTTAATA CCAGGAGGCA GTAAAATTCG TGTCACGAAC 
    GAAACGAAAC TGCGTTATTT GGATGCATTG GCACAACACA GACTAGCCAG TTCTATACGA 
    AACGAGGTAG AACATTTTCT AAGGGGCCTG AACGAGCTCA TCCCCGACAA CCTTTTAGGA 
    ATATTCGATG AAAATGAACT AGAATTGTTA CTATGTGGAA CTGGAGAATA CAGCGTGGCA 
    GATCTACGAG CGCACCATAT AGCTAATGGG AGTTCTCCGG AGTTTCTCCG AGTTCTGGAT 
    TGGTTCTGGA CAGCAGTTAG TAATTTCACA CAGGAGGAGA TGGCCCGATT ACTACAATTC 
    ACTACAGGTT GCTCGCAATT ACCACCCGGT GGATTCCAAC AGCTAAATCC ACGATTTCAG 
    ATCACAGCAG CACCAACATT TGCTAATTTG CCTACAGCAC ATACTTGCTT CAATCAACTC 
    TGCTTACCAG ATTACGAGTG TTACGATCAC TTCGAACGTG CTTTATTATT AGCGATTAGC 
    GAGGGAACCG AAGGTTTTGG TATGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003708415.1
    CDS3411..6200
    Translation

    Target ORF information:

    RefSeq Version XM_003708367.2
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003708367.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCGGACA AAATTCGAGG CTGTCGAGAT GGACAACGCG ACAAAGATTT CTTTGCCGAC 
    GAGATATCGT CCATCGGCAT GTCGCTGCGG CCCAGCGGTA GCGCTTCTTC CGGCATTTCA 
    ACCCTTGCAA GTTGCGGCGA AGTGGACGCG TTGCCAGAAC TCCCGGCACA GGACGAGGAA 
    CGATTGCAAC GCGCAGCGCG TTTGCTGCAA CAGAGACTCG TTTTACGTCA GTGGTTGACG 
    GACCATGGCC TGCATAGCCA CTACCAAAAG CTGATGCAGA TGGAAGTGAT GTCTCTCGAG 
    GACGTGTACT GGGTAGAAGA GAACACGGTA CGGGCAGCTT TTGGCAAAGA TCTACAACGG 
    TGGAACGAGG CCAGACAGAC ATTGCCTATT TCGAAGGAAG ATTTAGAAAC CCTCAAGGCT 
    GATCTTTGGA GCGCCGTTGT CAAGAACAGC CAACATCAAG ACGCCTGGAC ATGGGGTGGA 
    ATGTTAGTAG TTTCCGTGTC GGTAGCAGGG CTGGTGACCC TAGCTGCTAT GACACAGCCA 
    GCCCTAGCAC CAGAAGCAAA ACACTCCCTC CTGCAGTACG TAACCGGAAA GTATTTGTTA 
    CCAAGCAACT GCCGCGTTCA CTTCGAATGG GACGAGCCAC AGCTCGTAGG GGAGACAATG 
    ACTTTTACCG TGAAGTTTTA TCAACGCAAC GGCCAACCGT ATCCGATCTG TGACAAGGAC 
    AATTTAATCG TAGAAGTCAC AGAAGGCACG CGAAGGGTGG CGACCGTAAT TGAGTTGGGA 
    GGCACCGACC CGTTAGCTGC TAACACCGCA GTAGTCAAGT TTACTGTCCG CCATGCTGGA 
    CAATATCGAA TAGCCGTACT TATTGGTTCG TGTCACGTTC ACGGAAGCCC GTTTCTTAAG 
    AATTTTCTTC CTGGTCCTCC AGATCCAAAT AAGACAGTCT TCGTACGGCA AAGTTCTACG 
    GTAGTTTGTA CAGCTGGAAT TGCACACTCT ATGACAATAG AGCCACGAGA CGAATACGAT 
    AATTTGTGCG TATTTGGACC TGGAGATAAT CCTACGGAAG GATACCAAGT CAATATTACT 
    CAGATTGGGA ATGTAGTAGA TAAATCGGCG GTTACGAATT GTAGTATACA GCTTGATTAC 
    GACTCTCCGA ATCAAAGGAT TCGGTTGAAA GTTAGCTTCC CAAAGGGTGG TTGTTATCAC 
    GCGACAGTCA GTTTAGCTGG ACTGCAATTG CACAATGGGG ATTTCGACAT CATTGTACTC 
    GAAAGTGATG TTGCAAGAAT GGTACACAAC AACGTTGCTT CGAAGGACCC CGACATTTGT 
    TACGCTGCAA AATTGTTAGG CATGCAAGGC GAGAAATTTT CAAAGCCGAA AAAAGTTTTC 
    TGTTTTATTT CGCCTAAGCA GCTCACCATC AAGGAATATT TGTTGAGAAT AATACCGAAG 
    AGACTCGTTA CTTTCAGGCT TTGTCCTTCT ACCAAATTTC ATTTTGATTG TTCGAATAAT 
    CAATACGATG GTTACGACTC TTTCTCTATC GACGATGGAT GTCAGCCACC CGTCGAATTG 
    ATTTCTCTGT ACAGAGATAT AATAGCCGCT ACATTTACGT TGTTCCTTTT GAAGAACATA 
    GGTGGAAGCG AAACTTTTGC CGATAAACAA GATTTCTTTT ATCATGAAGT TCGAAAACAT 
    CATCAGAAAC ATTATCACGA AAAACTTTCG ATGAAAGTTC AAAGGGACAA ATTACTGGAA 
    TCGTCTATGA AAGCCACCAA AGGATTTTCC GTGAGCGACT GGTGCAGAAA TTTCGAAATC 
    ACTTTCCAAG GTGAACAAGG CGTCGATTGG GGAGGCGTTA GACGAGAATG GTTCGAACTA 
    ATTTGCGCGG CTCTATTCGA TTCAGGAAAC GGCTTATTCG CTTCCTTTGG AGAATCCCAG 
    CAAGCTTTGG TACATCCCAA TAGTAAACGG CCTCAACACC TCAAGTTAAA GCACTATGAG 
    TTTGCAGGTC GAATCGTGGG CAAATGCCTG TATGAATCTG CACTTGGTGG TTCCTATCGC 
    CAATTAGTTC GTGCAAGATT TACAAGATCA TTTCTTGCGC AAATCATAGG CCTCAGGGTA 
    CACTACAAGT ATTTCGAGCA GGATGACCCA GACCTATACC TAAGCAAGAT AAAATATATT 
    CTTGAGAACG ACGTGGAGGA AATGGAATTG TATTTTGTTG AAGAAGAGTA CGATAAAGAT 
    GGTCAATTAT TAAAAGTAGC CGAGTTAATA CCAGGAGGCA GTAAAATTCG TGTCACGAAC 
    GAAACGAAAC TGCGTTATTT GGATGCATTG GCACAACACA GACTAGCCAG TTCTATACGA 
    AACGAGGTAG AACATTTTCT AAGGGGCCTG AACGAGCTCA TCCCCGACAA CCTTTTAGGA 
    ATATTCGATG AAAATGAACT AGAATTGTTA CTATGTGGAA CTGGAGAATA CAGCGTGGCA 
    GATCTACGAG CGCACCATAT AGCTAATGGG AGTTCTCCGG AGTTTCTCCG AGTTCTGGAT 
    TGGTTCTGGA CAGCAGTTAG TAATTTCACA CAGGAGGAGA TGGCCCGATT ACTACAATTC 
    ACTACAGGTT GCTCGCAATT ACCACCCGGT GGATTCCAAC AGCTAAATCC ACGATTTCAG 
    ATCACAGCAG CACCAACATT TGCTAATTTG CCTACAGCAC ATACTTGCTT CAATCAACTC 
    TGCTTACCAG ATTACGAGTG TTACGATCAC TTCGAACGTG CTTTATTATT AGCGATTAGC 
    GAGGGAACCG AAGGTTTTGG TATGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100876415 ( XM_012297428.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012152818.1 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OMe115269
    Clone ID Related Accession (Same CDS sequence) XM_003708367.2 , XM_012297428.1
    Accession Version XM_012297428.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product apoptosis-resistant E3 ubiquitin protein ligase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003798699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCGGACA AAATTCGAGG CTGTCGAGAT GGACAACGCG ACAAAGATTT CTTTGCCGAC 
    GAGATATCGT CCATCGGCAT GTCGCTGCGG CCCAGCGGTA GCGCTTCTTC CGGCATTTCA 
    ACCCTTGCAA GTTGCGGCGA AGTGGACGCG TTGCCAGAAC TCCCGGCACA GGACGAGGAA 
    CGATTGCAAC GCGCAGCGCG TTTGCTGCAA CAGAGACTCG TTTTACGTCA GTGGTTGACG 
    GACCATGGCC TGCATAGCCA CTACCAAAAG CTGATGCAGA TGGAAGTGAT GTCTCTCGAG 
    GACGTGTACT GGGTAGAAGA GAACACGGTA CGGGCAGCTT TTGGCAAAGA TCTACAACGG 
    TGGAACGAGG CCAGACAGAC ATTGCCTATT TCGAAGGAAG ATTTAGAAAC CCTCAAGGCT 
    GATCTTTGGA GCGCCGTTGT CAAGAACAGC CAACATCAAG ACGCCTGGAC ATGGGGTGGA 
    ATGTTAGTAG TTTCCGTGTC GGTAGCAGGG CTGGTGACCC TAGCTGCTAT GACACAGCCA 
    GCCCTAGCAC CAGAAGCAAA ACACTCCCTC CTGCAGTACG TAACCGGAAA GTATTTGTTA 
    CCAAGCAACT GCCGCGTTCA CTTCGAATGG GACGAGCCAC AGCTCGTAGG GGAGACAATG 
    ACTTTTACCG TGAAGTTTTA TCAACGCAAC GGCCAACCGT ATCCGATCTG TGACAAGGAC 
    AATTTAATCG TAGAAGTCAC AGAAGGCACG CGAAGGGTGG CGACCGTAAT TGAGTTGGGA 
    GGCACCGACC CGTTAGCTGC TAACACCGCA GTAGTCAAGT TTACTGTCCG CCATGCTGGA 
    CAATATCGAA TAGCCGTACT TATTGGTTCG TGTCACGTTC ACGGAAGCCC GTTTCTTAAG 
    AATTTTCTTC CTGGTCCTCC AGATCCAAAT AAGACAGTCT TCGTACGGCA AAGTTCTACG 
    GTAGTTTGTA CAGCTGGAAT TGCACACTCT ATGACAATAG AGCCACGAGA CGAATACGAT 
    AATTTGTGCG TATTTGGACC TGGAGATAAT CCTACGGAAG GATACCAAGT CAATATTACT 
    CAGATTGGGA ATGTAGTAGA TAAATCGGCG GTTACGAATT GTAGTATACA GCTTGATTAC 
    GACTCTCCGA ATCAAAGGAT TCGGTTGAAA GTTAGCTTCC CAAAGGGTGG TTGTTATCAC 
    GCGACAGTCA GTTTAGCTGG ACTGCAATTG CACAATGGGG ATTTCGACAT CATTGTACTC 
    GAAAGTGATG TTGCAAGAAT GGTACACAAC AACGTTGCTT CGAAGGACCC CGACATTTGT 
    TACGCTGCAA AATTGTTAGG CATGCAAGGC GAGAAATTTT CAAAGCCGAA AAAAGTTTTC 
    TGTTTTATTT CGCCTAAGCA GCTCACCATC AAGGAATATT TGTTGAGAAT AATACCGAAG 
    AGACTCGTTA CTTTCAGGCT TTGTCCTTCT ACCAAATTTC ATTTTGATTG TTCGAATAAT 
    CAATACGATG GTTACGACTC TTTCTCTATC GACGATGGAT GTCAGCCACC CGTCGAATTG 
    ATTTCTCTGT ACAGAGATAT AATAGCCGCT ACATTTACGT TGTTCCTTTT GAAGAACATA 
    GGTGGAAGCG AAACTTTTGC CGATAAACAA GATTTCTTTT ATCATGAAGT TCGAAAACAT 
    CATCAGAAAC ATTATCACGA AAAACTTTCG ATGAAAGTTC AAAGGGACAA ATTACTGGAA 
    TCGTCTATGA AAGCCACCAA AGGATTTTCC GTGAGCGACT GGTGCAGAAA TTTCGAAATC 
    ACTTTCCAAG GTGAACAAGG CGTCGATTGG GGAGGCGTTA GACGAGAATG GTTCGAACTA 
    ATTTGCGCGG CTCTATTCGA TTCAGGAAAC GGCTTATTCG CTTCCTTTGG AGAATCCCAG 
    CAAGCTTTGG TACATCCCAA TAGTAAACGG CCTCAACACC TCAAGTTAAA GCACTATGAG 
    TTTGCAGGTC GAATCGTGGG CAAATGCCTG TATGAATCTG CACTTGGTGG TTCCTATCGC 
    CAATTAGTTC GTGCAAGATT TACAAGATCA TTTCTTGCGC AAATCATAGG CCTCAGGGTA 
    CACTACAAGT ATTTCGAGCA GGATGACCCA GACCTATACC TAAGCAAGAT AAAATATATT 
    CTTGAGAACG ACGTGGAGGA AATGGAATTG TATTTTGTTG AAGAAGAGTA CGATAAAGAT 
    GGTCAATTAT TAAAAGTAGC CGAGTTAATA CCAGGAGGCA GTAAAATTCG TGTCACGAAC 
    GAAACGAAAC TGCGTTATTT GGATGCATTG GCACAACACA GACTAGCCAG TTCTATACGA 
    AACGAGGTAG AACATTTTCT AAGGGGCCTG AACGAGCTCA TCCCCGACAA CCTTTTAGGA 
    ATATTCGATG AAAATGAACT AGAATTGTTA CTATGTGGAA CTGGAGAATA CAGCGTGGCA 
    GATCTACGAG CGCACCATAT AGCTAATGGG AGTTCTCCGG AGTTTCTCCG AGTTCTGGAT 
    TGGTTCTGGA CAGCAGTTAG TAATTTCACA CAGGAGGAGA TGGCCCGATT ACTACAATTC 
    ACTACAGGTT GCTCGCAATT ACCACCCGGT GGATTCCAAC AGCTAAATCC ACGATTTCAG 
    ATCACAGCAG CACCAACATT TGCTAATTTG CCTACAGCAC ATACTTGCTT CAATCAACTC 
    TGCTTACCAG ATTACGAGTG TTACGATCAC TTCGAACGTG CTTTATTATT AGCGATTAGC 
    GAGGGAACCG AAGGTTTTGG TATGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012152818.1
    CDS3414..6203
    Translation

    Target ORF information:

    RefSeq Version XM_012297428.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012297428.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGCCGGACA AAATTCGAGG CTGTCGAGAT GGACAACGCG ACAAAGATTT CTTTGCCGAC 
    GAGATATCGT CCATCGGCAT GTCGCTGCGG CCCAGCGGTA GCGCTTCTTC CGGCATTTCA 
    ACCCTTGCAA GTTGCGGCGA AGTGGACGCG TTGCCAGAAC TCCCGGCACA GGACGAGGAA 
    CGATTGCAAC GCGCAGCGCG TTTGCTGCAA CAGAGACTCG TTTTACGTCA GTGGTTGACG 
    GACCATGGCC TGCATAGCCA CTACCAAAAG CTGATGCAGA TGGAAGTGAT GTCTCTCGAG 
    GACGTGTACT GGGTAGAAGA GAACACGGTA CGGGCAGCTT TTGGCAAAGA TCTACAACGG 
    TGGAACGAGG CCAGACAGAC ATTGCCTATT TCGAAGGAAG ATTTAGAAAC CCTCAAGGCT 
    GATCTTTGGA GCGCCGTTGT CAAGAACAGC CAACATCAAG ACGCCTGGAC ATGGGGTGGA 
    ATGTTAGTAG TTTCCGTGTC GGTAGCAGGG CTGGTGACCC TAGCTGCTAT GACACAGCCA 
    GCCCTAGCAC CAGAAGCAAA ACACTCCCTC CTGCAGTACG TAACCGGAAA GTATTTGTTA 
    CCAAGCAACT GCCGCGTTCA CTTCGAATGG GACGAGCCAC AGCTCGTAGG GGAGACAATG 
    ACTTTTACCG TGAAGTTTTA TCAACGCAAC GGCCAACCGT ATCCGATCTG TGACAAGGAC 
    AATTTAATCG TAGAAGTCAC AGAAGGCACG CGAAGGGTGG CGACCGTAAT TGAGTTGGGA 
    GGCACCGACC CGTTAGCTGC TAACACCGCA GTAGTCAAGT TTACTGTCCG CCATGCTGGA 
    CAATATCGAA TAGCCGTACT TATTGGTTCG TGTCACGTTC ACGGAAGCCC GTTTCTTAAG 
    AATTTTCTTC CTGGTCCTCC AGATCCAAAT AAGACAGTCT TCGTACGGCA AAGTTCTACG 
    GTAGTTTGTA CAGCTGGAAT TGCACACTCT ATGACAATAG AGCCACGAGA CGAATACGAT 
    AATTTGTGCG TATTTGGACC TGGAGATAAT CCTACGGAAG GATACCAAGT CAATATTACT 
    CAGATTGGGA ATGTAGTAGA TAAATCGGCG GTTACGAATT GTAGTATACA GCTTGATTAC 
    GACTCTCCGA ATCAAAGGAT TCGGTTGAAA GTTAGCTTCC CAAAGGGTGG TTGTTATCAC 
    GCGACAGTCA GTTTAGCTGG ACTGCAATTG CACAATGGGG ATTTCGACAT CATTGTACTC 
    GAAAGTGATG TTGCAAGAAT GGTACACAAC AACGTTGCTT CGAAGGACCC CGACATTTGT 
    TACGCTGCAA AATTGTTAGG CATGCAAGGC GAGAAATTTT CAAAGCCGAA AAAAGTTTTC 
    TGTTTTATTT CGCCTAAGCA GCTCACCATC AAGGAATATT TGTTGAGAAT AATACCGAAG 
    AGACTCGTTA CTTTCAGGCT TTGTCCTTCT ACCAAATTTC ATTTTGATTG TTCGAATAAT 
    CAATACGATG GTTACGACTC TTTCTCTATC GACGATGGAT GTCAGCCACC CGTCGAATTG 
    ATTTCTCTGT ACAGAGATAT AATAGCCGCT ACATTTACGT TGTTCCTTTT GAAGAACATA 
    GGTGGAAGCG AAACTTTTGC CGATAAACAA GATTTCTTTT ATCATGAAGT TCGAAAACAT 
    CATCAGAAAC ATTATCACGA AAAACTTTCG ATGAAAGTTC AAAGGGACAA ATTACTGGAA 
    TCGTCTATGA AAGCCACCAA AGGATTTTCC GTGAGCGACT GGTGCAGAAA TTTCGAAATC 
    ACTTTCCAAG GTGAACAAGG CGTCGATTGG GGAGGCGTTA GACGAGAATG GTTCGAACTA 
    ATTTGCGCGG CTCTATTCGA TTCAGGAAAC GGCTTATTCG CTTCCTTTGG AGAATCCCAG 
    CAAGCTTTGG TACATCCCAA TAGTAAACGG CCTCAACACC TCAAGTTAAA GCACTATGAG 
    TTTGCAGGTC GAATCGTGGG CAAATGCCTG TATGAATCTG CACTTGGTGG TTCCTATCGC 
    CAATTAGTTC GTGCAAGATT TACAAGATCA TTTCTTGCGC AAATCATAGG CCTCAGGGTA 
    CACTACAAGT ATTTCGAGCA GGATGACCCA GACCTATACC TAAGCAAGAT AAAATATATT 
    CTTGAGAACG ACGTGGAGGA AATGGAATTG TATTTTGTTG AAGAAGAGTA CGATAAAGAT 
    GGTCAATTAT TAAAAGTAGC CGAGTTAATA CCAGGAGGCA GTAAAATTCG TGTCACGAAC 
    GAAACGAAAC TGCGTTATTT GGATGCATTG GCACAACACA GACTAGCCAG TTCTATACGA 
    AACGAGGTAG AACATTTTCT AAGGGGCCTG AACGAGCTCA TCCCCGACAA CCTTTTAGGA 
    ATATTCGATG AAAATGAACT AGAATTGTTA CTATGTGGAA CTGGAGAATA CAGCGTGGCA 
    GATCTACGAG CGCACCATAT AGCTAATGGG AGTTCTCCGG AGTTTCTCCG AGTTCTGGAT 
    TGGTTCTGGA CAGCAGTTAG TAATTTCACA CAGGAGGAGA TGGCCCGATT ACTACAATTC 
    ACTACAGGTT GCTCGCAATT ACCACCCGGT GGATTCCAAC AGCTAAATCC ACGATTTCAG 
    ATCACAGCAG CACCAACATT TGCTAATTTG CCTACAGCAC ATACTTGCTT CAATCAACTC 
    TGCTTACCAG ATTACGAGTG TTACGATCAC TTCGAACGTG CTTTATTATT AGCGATTAGC 
    GAGGGAACCG AAGGTTTTGG TATGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100876415 ( XM_012297429.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012152819.1 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OMe115270
    Clone ID Related Accession (Same CDS sequence) XM_012297429.1
    Accession Version XM_012297429.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product apoptosis-resistant E3 ubiquitin protein ligase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003798699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGATGC AGATGGAAGT GATGTCTCTC GAGGACGTGT ACTGGGTAGA AGAGAACACG 
    GTACGGGCAG CTTTTGGCAA AGATCTACAA CGGTGGAACG AGGCCAGACA GACATTGCCT 
    ATTTCGAAGG AAGATTTAGA AACCCTCAAG GCTGATCTTT GGAGCGCCGT TGTCAAGAAC 
    AGCCAACATC AAGACGCCTG GACATGGGGT GGAATGTTAG TAGTTTCCGT GTCGGTAGCA 
    GGGCTGGTGA CCCTAGCTGC TATGACACAG CCAGCCCTAG CACCAGAAGC AAAACACTCC 
    CTCCTGCAGT ACGTAACCGG AAAGTATTTG TTACCAAGCA ACTGCCGCGT TCACTTCGAA 
    TGGGACGAGC CACAGCTCGT AGGGGAGACA ATGACTTTTA CCGTGAAGTT TTATCAACGC 
    AACGGCCAAC CGTATCCGAT CTGTGACAAG GACAATTTAA TCGTAGAAGT CACAGAAGGC 
    ACGCGAAGGG TGGCGACCGT AATTGAGTTG GGAGGCACCG ACCCGTTAGC TGCTAACACC 
    GCAGTAGTCA AGTTTACTGT CCGCCATGCT GGACAATATC GAATAGCCGT ACTTATTGGT 
    TCGTGTCACG TTCACGGAAG CCCGTTTCTT AAGAATTTTC TTCCTGGTCC TCCAGATCCA 
    AATAAGACAG TCTTCGTACG GCAAAGTTCT ACGGTAGTTT GTACAGCTGG AATTGCACAC 
    TCTATGACAA TAGAGCCACG AGACGAATAC GATAATTTGT GCGTATTTGG ACCTGGAGAT 
    AATCCTACGG AAGGATACCA AGTCAATATT ACTCAGATTG GGAATGTAGT AGATAAATCG 
    GCGGTTACGA ATTGTAGTAT ACAGCTTGAT TACGACTCTC CGAATCAAAG GATTCGGTTG 
    AAAGTTAGCT TCCCAAAGGG TGGTTGTTAT CACGCGACAG TCAGTTTAGC TGGACTGCAA 
    TTGCACAATG GGGATTTCGA CATCATTGTA CTCGAAAGTG ATGTTGCAAG AATGGTACAC 
    AACAACGTTG CTTCGAAGGA CCCCGACATT TGTTACGCTG CAAAATTGTT AGGCATGCAA 
    GGCGAGAAAT TTTCAAAGCC GAAAAAAGTT TTCTGTTTTA TTTCGCCTAA GCAGCTCACC 
    ATCAAGGAAT ATTTGTTGAG AATAATACCG AAGAGACTCG TTACTTTCAG GCTTTGTCCT 
    TCTACCAAAT TTCATTTTGA TTGTTCGAAT AATCAATACG ATGGTTACGA CTCTTTCTCT 
    ATCGACGATG GATGTCAGCC ACCCGTCGAA TTGATTTCTC TGTACAGAGA TATAATAGCC 
    GCTACATTTA CGTTGTTCCT TTTGAAGAAC ATAGGTGGAA GCGAAACTTT TGCCGATAAA 
    CAAGATTTCT TTTATCATGA AGTTCGAAAA CATCATCAGA AACATTATCA CGAAAAACTT 
    TCGATGAAAG TTCAAAGGGA CAAATTACTG GAATCGTCTA TGAAAGCCAC CAAAGGATTT 
    TCCGTGAGCG ACTGGTGCAG AAATTTCGAA ATCACTTTCC AAGGTGAACA AGGCGTCGAT 
    TGGGGAGGCG TTAGACGAGA ATGGTTCGAA CTAATTTGCG CGGCTCTATT CGATTCAGGA 
    AACGGCTTAT TCGCTTCCTT TGGAGAATCC CAGCAAGCTT TGGTACATCC CAATAGTAAA 
    CGGCCTCAAC ACCTCAAGTT AAAGCACTAT GAGTTTGCAG GTCGAATCGT GGGCAAATGC 
    CTGTATGAAT CTGCACTTGG TGGTTCCTAT CGCCAATTAG TTCGTGCAAG ATTTACAAGA 
    TCATTTCTTG CGCAAATCAT AGGCCTCAGG GTACACTACA AGTATTTCGA GCAGGATGAC 
    CCAGACCTAT ACCTAAGCAA GATAAAATAT ATTCTTGAGA ACGACGTGGA GGAAATGGAA 
    TTGTATTTTG TTGAAGAAGA GTACGATAAA GATGGTCAAT TATTAAAAGT AGCCGAGTTA 
    ATACCAGGAG GCAGTAAAAT TCGTGTCACG AACGAAACGA AACTGCGTTA TTTGGATGCA 
    TTGGCACAAC ACAGACTAGC CAGTTCTATA CGAAACGAGG TAGAACATTT TCTAAGGGGC 
    CTGAACGAGC TCATCCCCGA CAACCTTTTA GGAATATTCG ATGAAAATGA ACTAGAATTG 
    TTACTATGTG GAACTGGAGA ATACAGCGTG GCAGATCTAC GAGCGCACCA TATAGCTAAT 
    GGGAGTTCTC CGGAGTTTCT CCGAGTTCTG GATTGGTTCT GGACAGCAGT TAGTAATTTC 
    ACACAGGAGG AGATGGCCCG ATTACTACAA TTCACTACAG GTTGCTCGCA ATTACCACCC 
    GGTGGATTCC AACAGCTAAA TCCACGATTT CAGATCACAG CAGCACCAAC ATTTGCTAAT 
    TTGCCTACAG CACATACTTG CTTCAATCAA CTCTGCTTAC CAGATTACGA GTGTTACGAT 
    CACTTCGAAC GTGCTTTATT ATTAGCGATT AGCGAGGGAA CCGAAGGTTT TGGTATGATT 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012152819.1
    CDS89..2611
    Translation

    Target ORF information:

    RefSeq Version XM_012297429.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012297429.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCTGATGC AGATGGAAGT GATGTCTCTC GAGGACGTGT ACTGGGTAGA AGAGAACACG 
    GTACGGGCAG CTTTTGGCAA AGATCTACAA CGGTGGAACG AGGCCAGACA GACATTGCCT 
    ATTTCGAAGG AAGATTTAGA AACCCTCAAG GCTGATCTTT GGAGCGCCGT TGTCAAGAAC 
    AGCCAACATC AAGACGCCTG GACATGGGGT GGAATGTTAG TAGTTTCCGT GTCGGTAGCA 
    GGGCTGGTGA CCCTAGCTGC TATGACACAG CCAGCCCTAG CACCAGAAGC AAAACACTCC 
    CTCCTGCAGT ACGTAACCGG AAAGTATTTG TTACCAAGCA ACTGCCGCGT TCACTTCGAA 
    TGGGACGAGC CACAGCTCGT AGGGGAGACA ATGACTTTTA CCGTGAAGTT TTATCAACGC 
    AACGGCCAAC CGTATCCGAT CTGTGACAAG GACAATTTAA TCGTAGAAGT CACAGAAGGC 
    ACGCGAAGGG TGGCGACCGT AATTGAGTTG GGAGGCACCG ACCCGTTAGC TGCTAACACC 
    GCAGTAGTCA AGTTTACTGT CCGCCATGCT GGACAATATC GAATAGCCGT ACTTATTGGT 
    TCGTGTCACG TTCACGGAAG CCCGTTTCTT AAGAATTTTC TTCCTGGTCC TCCAGATCCA 
    AATAAGACAG TCTTCGTACG GCAAAGTTCT ACGGTAGTTT GTACAGCTGG AATTGCACAC 
    TCTATGACAA TAGAGCCACG AGACGAATAC GATAATTTGT GCGTATTTGG ACCTGGAGAT 
    AATCCTACGG AAGGATACCA AGTCAATATT ACTCAGATTG GGAATGTAGT AGATAAATCG 
    GCGGTTACGA ATTGTAGTAT ACAGCTTGAT TACGACTCTC CGAATCAAAG GATTCGGTTG 
    AAAGTTAGCT TCCCAAAGGG TGGTTGTTAT CACGCGACAG TCAGTTTAGC TGGACTGCAA 
    TTGCACAATG GGGATTTCGA CATCATTGTA CTCGAAAGTG ATGTTGCAAG AATGGTACAC 
    AACAACGTTG CTTCGAAGGA CCCCGACATT TGTTACGCTG CAAAATTGTT AGGCATGCAA 
    GGCGAGAAAT TTTCAAAGCC GAAAAAAGTT TTCTGTTTTA TTTCGCCTAA GCAGCTCACC 
    ATCAAGGAAT ATTTGTTGAG AATAATACCG AAGAGACTCG TTACTTTCAG GCTTTGTCCT 
    TCTACCAAAT TTCATTTTGA TTGTTCGAAT AATCAATACG ATGGTTACGA CTCTTTCTCT 
    ATCGACGATG GATGTCAGCC ACCCGTCGAA TTGATTTCTC TGTACAGAGA TATAATAGCC 
    GCTACATTTA CGTTGTTCCT TTTGAAGAAC ATAGGTGGAA GCGAAACTTT TGCCGATAAA 
    CAAGATTTCT TTTATCATGA AGTTCGAAAA CATCATCAGA AACATTATCA CGAAAAACTT 
    TCGATGAAAG TTCAAAGGGA CAAATTACTG GAATCGTCTA TGAAAGCCAC CAAAGGATTT 
    TCCGTGAGCG ACTGGTGCAG AAATTTCGAA ATCACTTTCC AAGGTGAACA AGGCGTCGAT 
    TGGGGAGGCG TTAGACGAGA ATGGTTCGAA CTAATTTGCG CGGCTCTATT CGATTCAGGA 
    AACGGCTTAT TCGCTTCCTT TGGAGAATCC CAGCAAGCTT TGGTACATCC CAATAGTAAA 
    CGGCCTCAAC ACCTCAAGTT AAAGCACTAT GAGTTTGCAG GTCGAATCGT GGGCAAATGC 
    CTGTATGAAT CTGCACTTGG TGGTTCCTAT CGCCAATTAG TTCGTGCAAG ATTTACAAGA 
    TCATTTCTTG CGCAAATCAT AGGCCTCAGG GTACACTACA AGTATTTCGA GCAGGATGAC 
    CCAGACCTAT ACCTAAGCAA GATAAAATAT ATTCTTGAGA ACGACGTGGA GGAAATGGAA 
    TTGTATTTTG TTGAAGAAGA GTACGATAAA GATGGTCAAT TATTAAAAGT AGCCGAGTTA 
    ATACCAGGAG GCAGTAAAAT TCGTGTCACG AACGAAACGA AACTGCGTTA TTTGGATGCA 
    TTGGCACAAC ACAGACTAGC CAGTTCTATA CGAAACGAGG TAGAACATTT TCTAAGGGGC 
    CTGAACGAGC TCATCCCCGA CAACCTTTTA GGAATATTCG ATGAAAATGA ACTAGAATTG 
    TTACTATGTG GAACTGGAGA ATACAGCGTG GCAGATCTAC GAGCGCACCA TATAGCTAAT 
    GGGAGTTCTC CGGAGTTTCT CCGAGTTCTG GATTGGTTCT GGACAGCAGT TAGTAATTTC 
    ACACAGGAGG AGATGGCCCG ATTACTACAA TTCACTACAG GTTGCTCGCA ATTACCACCC 
    GGTGGATTCC AACAGCTAAA TCCACGATTT CAGATCACAG CAGCACCAAC ATTTGCTAAT 
    TTGCCTACAG CACATACTTG CTTCAATCAA CTCTGCTTAC CAGATTACGA GTGTTACGAT 
    CACTTCGAAC GTGCTTTATT ATTAGCGATT AGCGAGGGAA CCGAAGGTTT TGGTATGATT 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100876415 ( XM_012297430.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012152820.1 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OMe115271
    Clone ID Related Accession (Same CDS sequence) XM_012297430.1 , XM_012297433.1
    Accession Version XM_012297430.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product apoptosis-resistant E3 ubiquitin protein ligase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003798699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGATGG AAGTGATGTC TCTCGAGGAC GTGTACTGGG TAGAAGAGAA CACGGTACGG 
    GCAGCTTTTG GCAAAGATCT ACAACGGTGG AACGAGGCCA GACAGACATT GCCTATTTCG 
    AAGGAAGATT TAGAAACCCT CAAGGCTGAT CTTTGGAGCG CCGTTGTCAA GAACAGCCAA 
    CATCAAGACG CCTGGACATG GGGTGGAATG TTAGTAGTTT CCGTGTCGGT AGCAGGGCTG 
    GTGACCCTAG CTGCTATGAC ACAGCCAGCC CTAGCACCAG AAGCAAAACA CTCCCTCCTG 
    CAGTACGTAA CCGGAAAGTA TTTGTTACCA AGCAACTGCC GCGTTCACTT CGAATGGGAC 
    GAGCCACAGC TCGTAGGGGA GACAATGACT TTTACCGTGA AGTTTTATCA ACGCAACGGC 
    CAACCGTATC CGATCTGTGA CAAGGACAAT TTAATCGTAG AAGTCACAGA AGGCACGCGA 
    AGGGTGGCGA CCGTAATTGA GTTGGGAGGC ACCGACCCGT TAGCTGCTAA CACCGCAGTA 
    GTCAAGTTTA CTGTCCGCCA TGCTGGACAA TATCGAATAG CCGTACTTAT TGGTTCGTGT 
    CACGTTCACG GAAGCCCGTT TCTTAAGAAT TTTCTTCCTG GTCCTCCAGA TCCAAATAAG 
    ACAGTCTTCG TACGGCAAAG TTCTACGGTA GTTTGTACAG CTGGAATTGC ACACTCTATG 
    ACAATAGAGC CACGAGACGA ATACGATAAT TTGTGCGTAT TTGGACCTGG AGATAATCCT 
    ACGGAAGGAT ACCAAGTCAA TATTACTCAG ATTGGGAATG TAGTAGATAA ATCGGCGGTT 
    ACGAATTGTA GTATACAGCT TGATTACGAC TCTCCGAATC AAAGGATTCG GTTGAAAGTT 
    AGCTTCCCAA AGGGTGGTTG TTATCACGCG ACAGTCAGTT TAGCTGGACT GCAATTGCAC 
    AATGGGGATT TCGACATCAT TGTACTCGAA AGTGATGTTG CAAGAATGGT ACACAACAAC 
    GTTGCTTCGA AGGACCCCGA CATTTGTTAC GCTGCAAAAT TGTTAGGCAT GCAAGGCGAG 
    AAATTTTCAA AGCCGAAAAA AGTTTTCTGT TTTATTTCGC CTAAGCAGCT CACCATCAAG 
    GAATATTTGT TGAGAATAAT ACCGAAGAGA CTCGTTACTT TCAGGCTTTG TCCTTCTACC 
    AAATTTCATT TTGATTGTTC GAATAATCAA TACGATGGTT ACGACTCTTT CTCTATCGAC 
    GATGGATGTC AGCCACCCGT CGAATTGATT TCTCTGTACA GAGATATAAT AGCCGCTACA 
    TTTACGTTGT TCCTTTTGAA GAACATAGGT GGAAGCGAAA CTTTTGCCGA TAAACAAGAT 
    TTCTTTTATC ATGAAGTTCG AAAACATCAT CAGAAACATT ATCACGAAAA ACTTTCGATG 
    AAAGTTCAAA GGGACAAATT ACTGGAATCG TCTATGAAAG CCACCAAAGG ATTTTCCGTG 
    AGCGACTGGT GCAGAAATTT CGAAATCACT TTCCAAGGTG AACAAGGCGT CGATTGGGGA 
    GGCGTTAGAC GAGAATGGTT CGAACTAATT TGCGCGGCTC TATTCGATTC AGGAAACGGC 
    TTATTCGCTT CCTTTGGAGA ATCCCAGCAA GCTTTGGTAC ATCCCAATAG TAAACGGCCT 
    CAACACCTCA AGTTAAAGCA CTATGAGTTT GCAGGTCGAA TCGTGGGCAA ATGCCTGTAT 
    GAATCTGCAC TTGGTGGTTC CTATCGCCAA TTAGTTCGTG CAAGATTTAC AAGATCATTT 
    CTTGCGCAAA TCATAGGCCT CAGGGTACAC TACAAGTATT TCGAGCAGGA TGACCCAGAC 
    CTATACCTAA GCAAGATAAA ATATATTCTT GAGAACGACG TGGAGGAAAT GGAATTGTAT 
    TTTGTTGAAG AAGAGTACGA TAAAGATGGT CAATTATTAA AAGTAGCCGA GTTAATACCA 
    GGAGGCAGTA AAATTCGTGT CACGAACGAA ACGAAACTGC GTTATTTGGA TGCATTGGCA 
    CAACACAGAC TAGCCAGTTC TATACGAAAC GAGGTAGAAC ATTTTCTAAG GGGCCTGAAC 
    GAGCTCATCC CCGACAACCT TTTAGGAATA TTCGATGAAA ATGAACTAGA ATTGTTACTA 
    TGTGGAACTG GAGAATACAG CGTGGCAGAT CTACGAGCGC ACCATATAGC TAATGGGAGT 
    TCTCCGGAGT TTCTCCGAGT TCTGGATTGG TTCTGGACAG CAGTTAGTAA TTTCACACAG 
    GAGGAGATGG CCCGATTACT ACAATTCACT ACAGGTTGCT CGCAATTACC ACCCGGTGGA 
    TTCCAACAGC TAAATCCACG ATTTCAGATC ACAGCAGCAC CAACATTTGC TAATTTGCCT 
    ACAGCACATA CTTGCTTCAA TCAACTCTGC TTACCAGATT ACGAGTGTTA CGATCACTTC 
    GAACGTGCTT TATTATTAGC GATTAGCGAG GGAACCGAAG GTTTTGGTAT GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012152820.1
    CDS139..2655
    Translation

    Target ORF information:

    RefSeq Version XM_012297430.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012297430.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGATGG AAGTGATGTC TCTCGAGGAC GTGTACTGGG TAGAAGAGAA CACGGTACGG 
    GCAGCTTTTG GCAAAGATCT ACAACGGTGG AACGAGGCCA GACAGACATT GCCTATTTCG 
    AAGGAAGATT TAGAAACCCT CAAGGCTGAT CTTTGGAGCG CCGTTGTCAA GAACAGCCAA 
    CATCAAGACG CCTGGACATG GGGTGGAATG TTAGTAGTTT CCGTGTCGGT AGCAGGGCTG 
    GTGACCCTAG CTGCTATGAC ACAGCCAGCC CTAGCACCAG AAGCAAAACA CTCCCTCCTG 
    CAGTACGTAA CCGGAAAGTA TTTGTTACCA AGCAACTGCC GCGTTCACTT CGAATGGGAC 
    GAGCCACAGC TCGTAGGGGA GACAATGACT TTTACCGTGA AGTTTTATCA ACGCAACGGC 
    CAACCGTATC CGATCTGTGA CAAGGACAAT TTAATCGTAG AAGTCACAGA AGGCACGCGA 
    AGGGTGGCGA CCGTAATTGA GTTGGGAGGC ACCGACCCGT TAGCTGCTAA CACCGCAGTA 
    GTCAAGTTTA CTGTCCGCCA TGCTGGACAA TATCGAATAG CCGTACTTAT TGGTTCGTGT 
    CACGTTCACG GAAGCCCGTT TCTTAAGAAT TTTCTTCCTG GTCCTCCAGA TCCAAATAAG 
    ACAGTCTTCG TACGGCAAAG TTCTACGGTA GTTTGTACAG CTGGAATTGC ACACTCTATG 
    ACAATAGAGC CACGAGACGA ATACGATAAT TTGTGCGTAT TTGGACCTGG AGATAATCCT 
    ACGGAAGGAT ACCAAGTCAA TATTACTCAG ATTGGGAATG TAGTAGATAA ATCGGCGGTT 
    ACGAATTGTA GTATACAGCT TGATTACGAC TCTCCGAATC AAAGGATTCG GTTGAAAGTT 
    AGCTTCCCAA AGGGTGGTTG TTATCACGCG ACAGTCAGTT TAGCTGGACT GCAATTGCAC 
    AATGGGGATT TCGACATCAT TGTACTCGAA AGTGATGTTG CAAGAATGGT ACACAACAAC 
    GTTGCTTCGA AGGACCCCGA CATTTGTTAC GCTGCAAAAT TGTTAGGCAT GCAAGGCGAG 
    AAATTTTCAA AGCCGAAAAA AGTTTTCTGT TTTATTTCGC CTAAGCAGCT CACCATCAAG 
    GAATATTTGT TGAGAATAAT ACCGAAGAGA CTCGTTACTT TCAGGCTTTG TCCTTCTACC 
    AAATTTCATT TTGATTGTTC GAATAATCAA TACGATGGTT ACGACTCTTT CTCTATCGAC 
    GATGGATGTC AGCCACCCGT CGAATTGATT TCTCTGTACA GAGATATAAT AGCCGCTACA 
    TTTACGTTGT TCCTTTTGAA GAACATAGGT GGAAGCGAAA CTTTTGCCGA TAAACAAGAT 
    TTCTTTTATC ATGAAGTTCG AAAACATCAT CAGAAACATT ATCACGAAAA ACTTTCGATG 
    AAAGTTCAAA GGGACAAATT ACTGGAATCG TCTATGAAAG CCACCAAAGG ATTTTCCGTG 
    AGCGACTGGT GCAGAAATTT CGAAATCACT TTCCAAGGTG AACAAGGCGT CGATTGGGGA 
    GGCGTTAGAC GAGAATGGTT CGAACTAATT TGCGCGGCTC TATTCGATTC AGGAAACGGC 
    TTATTCGCTT CCTTTGGAGA ATCCCAGCAA GCTTTGGTAC ATCCCAATAG TAAACGGCCT 
    CAACACCTCA AGTTAAAGCA CTATGAGTTT GCAGGTCGAA TCGTGGGCAA ATGCCTGTAT 
    GAATCTGCAC TTGGTGGTTC CTATCGCCAA TTAGTTCGTG CAAGATTTAC AAGATCATTT 
    CTTGCGCAAA TCATAGGCCT CAGGGTACAC TACAAGTATT TCGAGCAGGA TGACCCAGAC 
    CTATACCTAA GCAAGATAAA ATATATTCTT GAGAACGACG TGGAGGAAAT GGAATTGTAT 
    TTTGTTGAAG AAGAGTACGA TAAAGATGGT CAATTATTAA AAGTAGCCGA GTTAATACCA 
    GGAGGCAGTA AAATTCGTGT CACGAACGAA ACGAAACTGC GTTATTTGGA TGCATTGGCA 
    CAACACAGAC TAGCCAGTTC TATACGAAAC GAGGTAGAAC ATTTTCTAAG GGGCCTGAAC 
    GAGCTCATCC CCGACAACCT TTTAGGAATA TTCGATGAAA ATGAACTAGA ATTGTTACTA 
    TGTGGAACTG GAGAATACAG CGTGGCAGAT CTACGAGCGC ACCATATAGC TAATGGGAGT 
    TCTCCGGAGT TTCTCCGAGT TCTGGATTGG TTCTGGACAG CAGTTAGTAA TTTCACACAG 
    GAGGAGATGG CCCGATTACT ACAATTCACT ACAGGTTGCT CGCAATTACC ACCCGGTGGA 
    TTCCAACAGC TAAATCCACG ATTTCAGATC ACAGCAGCAC CAACATTTGC TAATTTGCCT 
    ACAGCACATA CTTGCTTCAA TCAACTCTGC TTACCAGATT ACGAGTGTTA CGATCACTTC 
    GAACGTGCTT TATTATTAGC GATTAGCGAG GGAACCGAAG GTTTTGGTAT GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100876415 ( XM_012297433.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012152823.1 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OMe115271
    Clone ID Related Accession (Same CDS sequence) XM_012297430.1 , XM_012297433.1
    Accession Version XM_012297433.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2517bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product apoptosis-resistant E3 ubiquitin protein ligase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003798699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGATGG AAGTGATGTC TCTCGAGGAC GTGTACTGGG TAGAAGAGAA CACGGTACGG 
    GCAGCTTTTG GCAAAGATCT ACAACGGTGG AACGAGGCCA GACAGACATT GCCTATTTCG 
    AAGGAAGATT TAGAAACCCT CAAGGCTGAT CTTTGGAGCG CCGTTGTCAA GAACAGCCAA 
    CATCAAGACG CCTGGACATG GGGTGGAATG TTAGTAGTTT CCGTGTCGGT AGCAGGGCTG 
    GTGACCCTAG CTGCTATGAC ACAGCCAGCC CTAGCACCAG AAGCAAAACA CTCCCTCCTG 
    CAGTACGTAA CCGGAAAGTA TTTGTTACCA AGCAACTGCC GCGTTCACTT CGAATGGGAC 
    GAGCCACAGC TCGTAGGGGA GACAATGACT TTTACCGTGA AGTTTTATCA ACGCAACGGC 
    CAACCGTATC CGATCTGTGA CAAGGACAAT TTAATCGTAG AAGTCACAGA AGGCACGCGA 
    AGGGTGGCGA CCGTAATTGA GTTGGGAGGC ACCGACCCGT TAGCTGCTAA CACCGCAGTA 
    GTCAAGTTTA CTGTCCGCCA TGCTGGACAA TATCGAATAG CCGTACTTAT TGGTTCGTGT 
    CACGTTCACG GAAGCCCGTT TCTTAAGAAT TTTCTTCCTG GTCCTCCAGA TCCAAATAAG 
    ACAGTCTTCG TACGGCAAAG TTCTACGGTA GTTTGTACAG CTGGAATTGC ACACTCTATG 
    ACAATAGAGC CACGAGACGA ATACGATAAT TTGTGCGTAT TTGGACCTGG AGATAATCCT 
    ACGGAAGGAT ACCAAGTCAA TATTACTCAG ATTGGGAATG TAGTAGATAA ATCGGCGGTT 
    ACGAATTGTA GTATACAGCT TGATTACGAC TCTCCGAATC AAAGGATTCG GTTGAAAGTT 
    AGCTTCCCAA AGGGTGGTTG TTATCACGCG ACAGTCAGTT TAGCTGGACT GCAATTGCAC 
    AATGGGGATT TCGACATCAT TGTACTCGAA AGTGATGTTG CAAGAATGGT ACACAACAAC 
    GTTGCTTCGA AGGACCCCGA CATTTGTTAC GCTGCAAAAT TGTTAGGCAT GCAAGGCGAG 
    AAATTTTCAA AGCCGAAAAA AGTTTTCTGT TTTATTTCGC CTAAGCAGCT CACCATCAAG 
    GAATATTTGT TGAGAATAAT ACCGAAGAGA CTCGTTACTT TCAGGCTTTG TCCTTCTACC 
    AAATTTCATT TTGATTGTTC GAATAATCAA TACGATGGTT ACGACTCTTT CTCTATCGAC 
    GATGGATGTC AGCCACCCGT CGAATTGATT TCTCTGTACA GAGATATAAT AGCCGCTACA 
    TTTACGTTGT TCCTTTTGAA GAACATAGGT GGAAGCGAAA CTTTTGCCGA TAAACAAGAT 
    TTCTTTTATC ATGAAGTTCG AAAACATCAT CAGAAACATT ATCACGAAAA ACTTTCGATG 
    AAAGTTCAAA GGGACAAATT ACTGGAATCG TCTATGAAAG CCACCAAAGG ATTTTCCGTG 
    AGCGACTGGT GCAGAAATTT CGAAATCACT TTCCAAGGTG AACAAGGCGT CGATTGGGGA 
    GGCGTTAGAC GAGAATGGTT CGAACTAATT TGCGCGGCTC TATTCGATTC AGGAAACGGC 
    TTATTCGCTT CCTTTGGAGA ATCCCAGCAA GCTTTGGTAC ATCCCAATAG TAAACGGCCT 
    CAACACCTCA AGTTAAAGCA CTATGAGTTT GCAGGTCGAA TCGTGGGCAA ATGCCTGTAT 
    GAATCTGCAC TTGGTGGTTC CTATCGCCAA TTAGTTCGTG CAAGATTTAC AAGATCATTT 
    CTTGCGCAAA TCATAGGCCT CAGGGTACAC TACAAGTATT TCGAGCAGGA TGACCCAGAC 
    CTATACCTAA GCAAGATAAA ATATATTCTT GAGAACGACG TGGAGGAAAT GGAATTGTAT 
    TTTGTTGAAG AAGAGTACGA TAAAGATGGT CAATTATTAA AAGTAGCCGA GTTAATACCA 
    GGAGGCAGTA AAATTCGTGT CACGAACGAA ACGAAACTGC GTTATTTGGA TGCATTGGCA 
    CAACACAGAC TAGCCAGTTC TATACGAAAC GAGGTAGAAC ATTTTCTAAG GGGCCTGAAC 
    GAGCTCATCC CCGACAACCT TTTAGGAATA TTCGATGAAA ATGAACTAGA ATTGTTACTA 
    TGTGGAACTG GAGAATACAG CGTGGCAGAT CTACGAGCGC ACCATATAGC TAATGGGAGT 
    TCTCCGGAGT TTCTCCGAGT TCTGGATTGG TTCTGGACAG CAGTTAGTAA TTTCACACAG 
    GAGGAGATGG CCCGATTACT ACAATTCACT ACAGGTTGCT CGCAATTACC ACCCGGTGGA 
    TTCCAACAGC TAAATCCACG ATTTCAGATC ACAGCAGCAC CAACATTTGC TAATTTGCCT 
    ACAGCACATA CTTGCTTCAA TCAACTCTGC TTACCAGATT ACGAGTGTTA CGATCACTTC 
    GAACGTGCTT TATTATTAGC GATTAGCGAG GGAACCGAAG GTTTTGGTAT GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012152823.1
    CDS280..2796
    Translation

    Target ORF information:

    RefSeq Version XM_012297433.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012297433.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCAGATGG AAGTGATGTC TCTCGAGGAC GTGTACTGGG TAGAAGAGAA CACGGTACGG 
    GCAGCTTTTG GCAAAGATCT ACAACGGTGG AACGAGGCCA GACAGACATT GCCTATTTCG 
    AAGGAAGATT TAGAAACCCT CAAGGCTGAT CTTTGGAGCG CCGTTGTCAA GAACAGCCAA 
    CATCAAGACG CCTGGACATG GGGTGGAATG TTAGTAGTTT CCGTGTCGGT AGCAGGGCTG 
    GTGACCCTAG CTGCTATGAC ACAGCCAGCC CTAGCACCAG AAGCAAAACA CTCCCTCCTG 
    CAGTACGTAA CCGGAAAGTA TTTGTTACCA AGCAACTGCC GCGTTCACTT CGAATGGGAC 
    GAGCCACAGC TCGTAGGGGA GACAATGACT TTTACCGTGA AGTTTTATCA ACGCAACGGC 
    CAACCGTATC CGATCTGTGA CAAGGACAAT TTAATCGTAG AAGTCACAGA AGGCACGCGA 
    AGGGTGGCGA CCGTAATTGA GTTGGGAGGC ACCGACCCGT TAGCTGCTAA CACCGCAGTA 
    GTCAAGTTTA CTGTCCGCCA TGCTGGACAA TATCGAATAG CCGTACTTAT TGGTTCGTGT 
    CACGTTCACG GAAGCCCGTT TCTTAAGAAT TTTCTTCCTG GTCCTCCAGA TCCAAATAAG 
    ACAGTCTTCG TACGGCAAAG TTCTACGGTA GTTTGTACAG CTGGAATTGC ACACTCTATG 
    ACAATAGAGC CACGAGACGA ATACGATAAT TTGTGCGTAT TTGGACCTGG AGATAATCCT 
    ACGGAAGGAT ACCAAGTCAA TATTACTCAG ATTGGGAATG TAGTAGATAA ATCGGCGGTT 
    ACGAATTGTA GTATACAGCT TGATTACGAC TCTCCGAATC AAAGGATTCG GTTGAAAGTT 
    AGCTTCCCAA AGGGTGGTTG TTATCACGCG ACAGTCAGTT TAGCTGGACT GCAATTGCAC 
    AATGGGGATT TCGACATCAT TGTACTCGAA AGTGATGTTG CAAGAATGGT ACACAACAAC 
    GTTGCTTCGA AGGACCCCGA CATTTGTTAC GCTGCAAAAT TGTTAGGCAT GCAAGGCGAG 
    AAATTTTCAA AGCCGAAAAA AGTTTTCTGT TTTATTTCGC CTAAGCAGCT CACCATCAAG 
    GAATATTTGT TGAGAATAAT ACCGAAGAGA CTCGTTACTT TCAGGCTTTG TCCTTCTACC 
    AAATTTCATT TTGATTGTTC GAATAATCAA TACGATGGTT ACGACTCTTT CTCTATCGAC 
    GATGGATGTC AGCCACCCGT CGAATTGATT TCTCTGTACA GAGATATAAT AGCCGCTACA 
    TTTACGTTGT TCCTTTTGAA GAACATAGGT GGAAGCGAAA CTTTTGCCGA TAAACAAGAT 
    TTCTTTTATC ATGAAGTTCG AAAACATCAT CAGAAACATT ATCACGAAAA ACTTTCGATG 
    AAAGTTCAAA GGGACAAATT ACTGGAATCG TCTATGAAAG CCACCAAAGG ATTTTCCGTG 
    AGCGACTGGT GCAGAAATTT CGAAATCACT TTCCAAGGTG AACAAGGCGT CGATTGGGGA 
    GGCGTTAGAC GAGAATGGTT CGAACTAATT TGCGCGGCTC TATTCGATTC AGGAAACGGC 
    TTATTCGCTT CCTTTGGAGA ATCCCAGCAA GCTTTGGTAC ATCCCAATAG TAAACGGCCT 
    CAACACCTCA AGTTAAAGCA CTATGAGTTT GCAGGTCGAA TCGTGGGCAA ATGCCTGTAT 
    GAATCTGCAC TTGGTGGTTC CTATCGCCAA TTAGTTCGTG CAAGATTTAC AAGATCATTT 
    CTTGCGCAAA TCATAGGCCT CAGGGTACAC TACAAGTATT TCGAGCAGGA TGACCCAGAC 
    CTATACCTAA GCAAGATAAA ATATATTCTT GAGAACGACG TGGAGGAAAT GGAATTGTAT 
    TTTGTTGAAG AAGAGTACGA TAAAGATGGT CAATTATTAA AAGTAGCCGA GTTAATACCA 
    GGAGGCAGTA AAATTCGTGT CACGAACGAA ACGAAACTGC GTTATTTGGA TGCATTGGCA 
    CAACACAGAC TAGCCAGTTC TATACGAAAC GAGGTAGAAC ATTTTCTAAG GGGCCTGAAC 
    GAGCTCATCC CCGACAACCT TTTAGGAATA TTCGATGAAA ATGAACTAGA ATTGTTACTA 
    TGTGGAACTG GAGAATACAG CGTGGCAGAT CTACGAGCGC ACCATATAGC TAATGGGAGT 
    TCTCCGGAGT TTCTCCGAGT TCTGGATTGG TTCTGGACAG CAGTTAGTAA TTTCACACAG 
    GAGGAGATGG CCCGATTACT ACAATTCACT ACAGGTTGCT CGCAATTACC ACCCGGTGGA 
    TTCCAACAGC TAAATCCACG ATTTCAGATC ACAGCAGCAC CAACATTTGC TAATTTGCCT 
    ACAGCACATA CTTGCTTCAA TCAACTCTGC TTACCAGATT ACGAGTGTTA CGATCACTTC 
    GAACGTGCTT TATTATTAGC GATTAGCGAG GGAACCGAAG GTTTTGGTAT GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC100876415 ( XM_012297431.1 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_012152821.1 Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OMe115272
    Clone ID Related Accession (Same CDS sequence) XM_012297431.1
    Accession Version XM_012297431.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2505bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-04-06
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Product apoptosis-resistant E3 ubiquitin protein ligase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003798699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCACGAT GGTCGACTGT ATTGTCGGGG GTATTCCTGG CCCTTTCTAT GGTCCTGTCG 
    CTTGGCAAAC TTCTGATGCT TGCCACCGGA AATGGAAACA ACGGGGATCT CACCGAAGCG 
    GATCAGTGGC TCGCTGAAAT TGGCCTAAAA CAATACCGGC CTCTCTTCAA AAAGAAAGGC 
    ATTTCAACCC TTGCAAGTTG CGGCGAAGTG GACGCGTTGC CAGAACTCCC GGCACAGGAC 
    GAGGAACGAT TGCAACGCGC AGCGCGTTTG CTGCAACAGA GACTCGTTTT ACGTCAGTGG 
    TTGACGGACC ATGGCCTGCA TAGCCACTAC CAAAAGCTGA TGCAGATGGA AGTGATGTCT 
    CTCGAGGACG TGTACTGGGT AGAAGAGAAC ACGGTACGGG CAGCTTTTGG CAAAGATCTA 
    CAACGGTGGA ACGAGGCCAG ACAGACATTG CCTATTTCGA AGGAAGATTT AGAAACCCTC 
    AAGGCTGATC TTTGGAGCGC CGTTGTCAAG AACAGCCAAC ATCAAGACGC CTGGACATGG 
    GGTGGAATGT TAGTAGTTTC CGTGTCGGTA GCAGGGCTGG TGACCCTAGC TGCTATGACA 
    CAGCCAGCCC TAGCACCAGA AGCAAAACAC TCCCTCCTGC AGTACGTAAC CGGAAAGTAT 
    TTGTTACCAA GCAACTGCCG CGTTCACTTC GAATGGGACG AGCCACAGCT CGTAGGGGAG 
    ACAATGACTT TTACCGTGAA GTTTTATCAA CGCAACGGCC AACCGTATCC GATCTGTGAC 
    AAGGACAATT TAATCGTAGA AGTCACAGAA GGCACGCGAA GGGTGGCGAC CGTAATTGAG 
    TTGGGAGGCA CCGACCCGTT AGCTGCTAAC ACCGCAGTAG TCAAGTTTAC TGTCCGCCAT 
    GCTGGACAAT ATCGAATAGC CGTACTTATT GGTTCGTGTC ACGTTCACGG AAGCCCGTTT 
    CTTAAGAATT TTCTTCCTGG TGATGTTGCA AGAATGGTAC ACAACAACGT TGCTTCGAAG 
    GACCCCGACA TTTGTTACGC TGCAAAATTG TTAGGCATGC AAGGCGAGAA ATTTTCAAAG 
    CCGAAAAAAG TTTTCTGTTT TATTTCGCCT AAGCAGCTCA CCATCAAGGA ATATTTGTTG 
    AGAATAATAC CGAAGAGACT CGTTACTTTC AGGCTTTGTC CTTCTACCAA ATTTCATTTT 
    GATTGTTCGA ATAATCAATA CGATGGTTAC GACTCTTTCT CTATCGACGA TGGATGTCAG 
    CCACCCGTCG AATTGATTTC TCTGTACAGA GATATAATAG CCGCTACATT TACGTTGTTC 
    CTTTTGAAGA ACATAGGTGG AAGCGAAACT TTTGCCGATA AACAAGATTT CTTTTATCAT 
    GAAGTTCGAA AACATCATCA GAAACATTAT CACGAAAAAC TTTCGATGAA AGTTCAAAGG 
    GACAAATTAC TGGAATCGTC TATGAAAGCC ACCAAAGGAT TTTCCGTGAG CGACTGGTGC 
    AGAAATTTCG AAATCACTTT CCAAGGTGAA CAAGGCGTCG ATTGGGGAGG CGTTAGACGA 
    GAATGGTTCG AACTAATTTG CGCGGCTCTA TTCGATTCAG GAAACGGCTT ATTCGCTTCC 
    TTTGGAGAAT CCCAGCAAGC TTTGGTACAT CCCAATAGTA AACGGCCTCA ACACCTCAAG 
    TTAAAGCACT ATGAGTTTGC AGGTCGAATC GTGGGCAAAT GCCTGTATGA ATCTGCACTT 
    GGTGGTTCCT ATCGCCAATT AGTTCGTGCA AGATTTACAA GATCATTTCT TGCGCAAATC 
    ATAGGCCTCA GGGTACACTA CAAGTATTTC GAGCAGGATG ACCCAGACCT ATACCTAAGC 
    AAGATAAAAT ATATTCTTGA GAACGACGTG GAGGAAATGG AATTGTATTT TGTTGAAGAA 
    GAGTACGATA AAGATGGTCA ATTATTAAAA GTAGCCGAGT TAATACCAGG AGGCAGTAAA 
    ATTCGTGTCA CGAACGAAAC GAAACTGCGT TATTTGGATG CATTGGCACA ACACAGACTA 
    GCCAGTTCTA TACGAAACGA GGTAGAACAT TTTCTAAGGG GCCTGAACGA GCTCATCCCC 
    GACAACCTTT TAGGAATATT CGATGAAAAT GAACTAGAAT TGTTACTATG TGGAACTGGA 
    GAATACAGCG TGGCAGATCT ACGAGCGCAC CATATAGCTA ATGGGAGTTC TCCGGAGTTT 
    CTCCGAGTTC TGGATTGGTT CTGGACAGCA GTTAGTAATT TCACACAGGA GGAGATGGCC 
    CGATTACTAC AATTCACTAC AGGTTGCTCG CAATTACCAC CCGGTGGATT CCAACAGCTA 
    AATCCACGAT TTCAGATCAC AGCAGCACCA ACATTTGCTA ATTTGCCTAC AGCACATACT 
    TGCTTCAATC AACTCTGCTT ACCAGATTAC GAGTGTTACG ATCACTTCGA ACGTGCTTTA 
    TTATTAGCGA TTAGCGAGGG AACCGAAGGT TTTGGTATGA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012152821.1
    CDS183..2687
    Translation

    Target ORF information:

    RefSeq Version XM_012297431.1
    Organism Megachile rotundata(alfalfa leafcutting bee)
    Definition Megachile rotundata apoptosis-resistant E3 ubiquitin protein ligase 1 (LOC100876415), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012297431.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCACGAT GGTCGACTGT ATTGTCGGGG GTATTCCTGG CCCTTTCTAT GGTCCTGTCG 
    CTTGGCAAAC TTCTGATGCT TGCCACCGGA AATGGAAACA ACGGGGATCT CACCGAAGCG 
    GATCAGTGGC TCGCTGAAAT TGGCCTAAAA CAATACCGGC CTCTCTTCAA AAAGAAAGGC 
    ATTTCAACCC TTGCAAGTTG CGGCGAAGTG GACGCGTTGC CAGAACTCCC GGCACAGGAC 
    GAGGAACGAT TGCAACGCGC AGCGCGTTTG CTGCAACAGA GACTCGTTTT ACGTCAGTGG 
    TTGACGGACC ATGGCCTGCA TAGCCACTAC CAAAAGCTGA TGCAGATGGA AGTGATGTCT 
    CTCGAGGACG TGTACTGGGT AGAAGAGAAC ACGGTACGGG CAGCTTTTGG CAAAGATCTA 
    CAACGGTGGA ACGAGGCCAG ACAGACATTG CCTATTTCGA AGGAAGATTT AGAAACCCTC 
    AAGGCTGATC TTTGGAGCGC CGTTGTCAAG AACAGCCAAC ATCAAGACGC CTGGACATGG 
    GGTGGAATGT TAGTAGTTTC CGTGTCGGTA GCAGGGCTGG TGACCCTAGC TGCTATGACA 
    CAGCCAGCCC TAGCACCAGA AGCAAAACAC TCCCTCCTGC AGTACGTAAC CGGAAAGTAT 
    TTGTTACCAA GCAACTGCCG CGTTCACTTC GAATGGGACG AGCCACAGCT CGTAGGGGAG 
    ACAATGACTT TTACCGTGAA GTTTTATCAA CGCAACGGCC AACCGTATCC GATCTGTGAC 
    AAGGACAATT TAATCGTAGA AGTCACAGAA GGCACGCGAA GGGTGGCGAC CGTAATTGAG 
    TTGGGAGGCA CCGACCCGTT AGCTGCTAAC ACCGCAGTAG TCAAGTTTAC TGTCCGCCAT 
    GCTGGACAAT ATCGAATAGC CGTACTTATT GGTTCGTGTC ACGTTCACGG AAGCCCGTTT 
    CTTAAGAATT TTCTTCCTGG TGATGTTGCA AGAATGGTAC ACAACAACGT TGCTTCGAAG 
    GACCCCGACA TTTGTTACGC TGCAAAATTG TTAGGCATGC AAGGCGAGAA ATTTTCAAAG 
    CCGAAAAAAG TTTTCTGTTT TATTTCGCCT AAGCAGCTCA CCATCAAGGA ATATTTGTTG 
    AGAATAATAC CGAAGAGACT CGTTACTTTC AGGCTTTGTC CTTCTACCAA ATTTCATTTT 
    GATTGTTCGA ATAATCAATA CGATGGTTAC GACTCTTTCT CTATCGACGA TGGATGTCAG 
    CCACCCGTCG AATTGATTTC TCTGTACAGA GATATAATAG CCGCTACATT TACGTTGTTC 
    CTTTTGAAGA ACATAGGTGG AAGCGAAACT TTTGCCGATA AACAAGATTT CTTTTATCAT 
    GAAGTTCGAA AACATCATCA GAAACATTAT CACGAAAAAC TTTCGATGAA AGTTCAAAGG 
    GACAAATTAC TGGAATCGTC TATGAAAGCC ACCAAAGGAT TTTCCGTGAG CGACTGGTGC 
    AGAAATTTCG AAATCACTTT CCAAGGTGAA CAAGGCGTCG ATTGGGGAGG CGTTAGACGA 
    GAATGGTTCG AACTAATTTG CGCGGCTCTA TTCGATTCAG GAAACGGCTT ATTCGCTTCC 
    TTTGGAGAAT CCCAGCAAGC TTTGGTACAT CCCAATAGTA AACGGCCTCA ACACCTCAAG 
    TTAAAGCACT ATGAGTTTGC AGGTCGAATC GTGGGCAAAT GCCTGTATGA ATCTGCACTT 
    GGTGGTTCCT ATCGCCAATT AGTTCGTGCA AGATTTACAA GATCATTTCT TGCGCAAATC 
    ATAGGCCTCA GGGTACACTA CAAGTATTTC GAGCAGGATG ACCCAGACCT ATACCTAAGC 
    AAGATAAAAT ATATTCTTGA GAACGACGTG GAGGAAATGG AATTGTATTT TGTTGAAGAA 
    GAGTACGATA AAGATGGTCA ATTATTAAAA GTAGCCGAGT TAATACCAGG AGGCAGTAAA 
    ATTCGTGTCA CGAACGAAAC GAAACTGCGT TATTTGGATG CATTGGCACA ACACAGACTA 
    GCCAGTTCTA TACGAAACGA GGTAGAACAT TTTCTAAGGG GCCTGAACGA GCTCATCCCC 
    GACAACCTTT TAGGAATATT CGATGAAAAT GAACTAGAAT TGTTACTATG TGGAACTGGA 
    GAATACAGCG TGGCAGATCT ACGAGCGCAC CATATAGCTA ATGGGAGTTC TCCGGAGTTT 
    CTCCGAGTTC TGGATTGGTT CTGGACAGCA GTTAGTAATT TCACACAGGA GGAGATGGCC 
    CGATTACTAC AATTCACTAC AGGTTGCTCG CAATTACCAC CCGGTGGATT CCAACAGCTA 
    AATCCACGAT TTCAGATCAC AGCAGCACCA ACATTTGCTA ATTTGCCTAC AGCACATACT 
    TGCTTCAATC AACTCTGCTT ACCAGATTAC GAGTGTTACG ATCACTTCGA ACGTGCTTTA 
    TTATTAGCGA TTAGCGAGGG AACCGAAGGT TTTGGTATGA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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