LOC100879442 cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100879442
Entrez Gene ID	100879442
Full Name	rRNA 2'-O-methyltransferase fibrillarin
Gene Type	protein-coding
Organism	Megachile rotundata(alfalfa leafcutting bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003700904.2	XP_003700952.1	rRNA 2'-O-methyltransferase fibrillarin

Pathways from BioSystems
Gene Ontology

The following LOC100879442 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100879442 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe99811	XM_003700904.2 Latest version!	Megachile rotundata rRNA 2'-O-methyltransferase fibrillarin (LOC100879442), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100879442 ( XM_003700904.2 ) cDNA ORF clone, Megachile rotundata(alfalfa leafcutting bee) -> XP_003700952.1 Megachile rotundata rRNA 2'-O-methyltransferase fibrillarin (LOC100879442), mRNA.

Price & Availability↑

CloneID	OMe99811
Clone ID Related Accession (Same CDS sequence)	XM_003700904.2
Accession Version	XM_003700904.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-06
Organism	Megachile rotundata(alfalfa leafcutting bee)
Product	rRNA 2'-O-methyltransferase fibrillarin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003797102.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2015 this sequence version replaced XM_003700904.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Megachile rotundata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAAC CAGGGTTTTC ACCAGGTGGA GGAGGTGGAG GTGGCAGAGG TGGAGGTTTT 
GGAGGTAGAG GAGGCGGAGG CGGTGGAGGC CGCGGTGGTG GATTTAAAGG AGGAAGAGGT 
GGAGGAGGTG GAGGTGGCAG AGGTGGAGGT TTTGGTGGTA GAGGAGGTGG CGGCAGAGGA 
GGCGGAAGAG GTGGAGGTAC TCCACGTGGA CGAGGTGGTG GTGGTGGTCG TGGAGGAAGA 
GGACGTGGAG GTGGAGGTTT CAAAGGAGGC AAAACTGTTG TCATAGAACC ACATCGTCAT 
GAGGGAGTTT TTATAGCCAG AGGAAAAGAA GATGCATTAG TTACATTAAA TTTAGTACCA 
GGTTCAGAAG TATATGGTGA GAAGAGAATC AGTGTTGAGG GTCAAAATGG AGAAAAGATT 
GAATACAGAG TATGGAATCC CTTTAGATCA AAATTAGCTG CAGCCATACT TGGAGGTGTA 
GACCAAATTC ACATGCCTCC TGGAAGCAAA GTCTTATATT TAGGTGCTGC ATCTGGAACT 
ACAGTATCAC ACGTAGCAGA TGTTGTTGGA CCAGAAGGAT TGGTATATGC TGTGGAATTT 
TCTCACAGAT CTGGTAGAGA TCTAATAAAT GTTGCTAAGA AACGGACAAA TATTATTCCT 
ATAATTGAAG ATGCAAGGCA TCCACACAAA TACAGAATGC TAGTTAGTAT GGTGGATACA 
ATATTCGCAG ATGTAGCACA ACCAGATCAG GCCAGAATAG TAGCATTAAA TGCTCAATAC 
TTCTTGAAAA ATGGTGGTCA CTTTGTTATT TCTATTAAGG CAAGTTGTAT AGACTCTACA 
GCTCAACCCG AAGCAGTATT CGCGGCAGAA GTAAAGAAAC TTATCGCGGA TAAATTGAAA 
CCACAAGAAC AAATTACATT AGAACCATAC GAAAGAGACC ACGCTGTAGT AGTTGGTGTC 
TACAGGCCAG CACCTAAAAA AGCAACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003700952.1
CDS	101..1090
Translation	MGKPGFSPGGGGGGGRGGGFGGRGGGGGGGRGGGFKGGRGGGGGGGRGGGFGGRGGGGRGGGRGGGTPRGRGGGGGRGGRGRGGGGFKGGKTVVIEPHRHEGVFIARGKEDALVTLNLVPGSEVYGEKRISVEGQNGEKIEYRVWNPFRSKLAAAILGGVDQIHMPPGSKVLYLGAASGTTVSHVADVVGPEGLVYAVEFSHRSGRDLINVAKKRTNIIPIIEDARHPHKYRMLVSMVDTIFADVAQPDQARIVALNAQYFLKNGGHFVISIKASCIDSTAQPEAVFAAEVKKLIADKLKPQEQITLEPYERDHAVVVGVYRPAPKKAT

Target ORF information:

RefSeq Version	XM_003700904.2
Organism	Megachile rotundata(alfalfa leafcutting bee)
Definition	Megachile rotundata rRNA 2'-O-methyltransferase fibrillarin (LOC100879442), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003700904.2

ORF Insert Sequence:

ATGGGGAAAC CAGGGTTTTC ACCAGGTGGA GGAGGTGGAG GTGGCAGAGG TGGAGGTTTT 
GGAGGTAGAG GAGGCGGAGG CGGTGGAGGC CGCGGTGGTG GATTTAAAGG AGGAAGAGGT 
GGAGGAGGTG GAGGTGGCAG AGGTGGAGGT TTTGGTGGTA GAGGAGGTGG CGGCAGAGGA 
GGCGGAAGAG GTGGAGGTAC TCCACGTGGA CGAGGTGGTG GTGGTGGTCG TGGAGGAAGA 
GGACGTGGAG GTGGAGGTTT CAAAGGAGGC AAAACTGTTG TCATAGAACC ACATCGTCAT 
GAGGGAGTTT TTATAGCCAG AGGAAAAGAA GATGCATTAG TTACATTAAA TTTAGTACCA 
GGTTCAGAAG TATATGGTGA GAAGAGAATC AGTGTTGAGG GTCAAAATGG AGAAAAGATT 
GAATACAGAG TATGGAATCC CTTTAGATCA AAATTAGCTG CAGCCATACT TGGAGGTGTA 
GACCAAATTC ACATGCCTCC TGGAAGCAAA GTCTTATATT TAGGTGCTGC ATCTGGAACT 
ACAGTATCAC ACGTAGCAGA TGTTGTTGGA CCAGAAGGAT TGGTATATGC TGTGGAATTT 
TCTCACAGAT CTGGTAGAGA TCTAATAAAT GTTGCTAAGA AACGGACAAA TATTATTCCT 
ATAATTGAAG ATGCAAGGCA TCCACACAAA TACAGAATGC TAGTTAGTAT GGTGGATACA 
ATATTCGCAG ATGTAGCACA ACCAGATCAG GCCAGAATAG TAGCATTAAA TGCTCAATAC 
TTCTTGAAAA ATGGTGGTCA CTTTGTTATT TCTATTAAGG CAAGTTGTAT AGACTCTACA 
GCTCAACCCG AAGCAGTATT CGCGGCAGAA GTAAAGAAAC TTATCGCGGA TAAATTGAAA 
CCACAAGAAC AAATTACATT AGAACCATAC GAAAGAGACC ACGCTGTAGT AGTTGGTGTC 
TACAGGCCAG CACCTAAAAA AGCAACTTAA