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EIF2B3 cDNA ORF clone, Mus musculus(House mouse)

Summary

Gene Symbol EIF2B3
Entrez Gene ID 108067
Full Name eukaryotic translation initiation factor 2B, subunit 3
Synonyms 1190002P15Rik
General protein information
Preferred Names

eukaryotic translation initiation factor 2B, subunit 3

Names

translation initiation factor eIF-2B subunit gamma
Gene Type protein-coding
Organism Mus musculus(House mouse)
Genome

4

4|4 D1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001111277.1 NP_001104747.1 translation initiation factor eIF-2B subunit gamma isoform 1
NM_001111277.2 NP_001104747.1 translation initiation factor eIF-2B subunit gamma isoform 1
NM_001355225.1 NP_001342154.1 translation initiation factor eIF-2B subunit gamma isoform 3
XM_006502649.1 XP_006502712.1 translation initiation factor eIF-2B subunit gamma isoform X1
XM_006502649.2 XP_006502712.1 translation initiation factor eIF-2B subunit gamma isoform X1
NM_175135.5 NP_780344.2 translation initiation factor eIF-2B subunit gamma isoform 2
XM_006502650.1 XP_006502713.1 translation initiation factor eIF-2B subunit gamma isoform X2
NM_175135.4 NP_780344.2 translation initiation factor eIF-2B subunit gamma isoform 2

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) EIF2B3 XP_001152272.1
EIF2B3 XP_001094199.2
Bos taurus (cattle) EIF2B3 NP_001076943.1
Gallus gallus (chicken) EIF2B3 NP_001026451.1
Drosophila melanogaster (fruit fly) eIF2B-gamma NP_611046.2
Neurospora crassa NCU03548 XP_955772.2
Arabidopsis thaliana (thale cress) AT5G19485 NP_001031908.1
Homo sapiens (human) EIF2B3 NP_065098.1
Caenorhabditis elegans (roundworm) ppp-1 NP_495428.1
Schizosaccharomyces pombe (fission yeast) tif223 NP_594962.2
Canis lupus familiaris (dog) EIF2B3 XP_005629136.1
Mus musculus (house mouse) Eif2b3 NP_001104747.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP005210 XP_314112.3
Rattus norvegicus (Norway rat) Eif2b3 NP_598293.2
Danio rerio (zebrafish) eif2b3 NP_957368.1
Magnaporthe oryzae (rice blast fungus) MGG_02390 XP_003709167.1
Oryza sativa (rice) Os06g0338900 NP_001057561.1
Xenopus tropicalis (tropical clawed frog) eif2b3 NP_001016997.2

Gene Ontology


Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, N?rnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Doll? P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A
PLoS biology9(1)e1000582(2011 Jan)

Large-scale gene trapping in C57BL/6N mouse embryonic stem cells.
Hansen GM, Markesich DC, Burnett MB, Zhu Q, Dionne KM, Richter LJ, Finnell RH, Sands AT, Zambrowicz BP, Abuin A
Genome research18(10)1670-9(2008 Oct)

EUCOMM--the European conditional mouse mutagenesis program.
Friedel RH, Seisenberger C, Kaloff C, Wurst W
Briefings in functional genomics & proteomics6(3)180-5(2007 Sep)

BGEM: an in situ hybridization database of gene expression in the embryonic and adult mouse nervous system.
Magdaleno S, Jensen P, Brumwell CL, Seal A, Lehman K, Asbury A, Cheung T, Cornelius T, Batten DM, Eden C, Norland SM, Rice DS, Dosooye N, Shakya S, Mehta P, Curran T
PLoS biology4(4)e86(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EIF2B3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF2B3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu33275 NM_001355225.1
Latest version!		 Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu33275 XM_006502650.1
Latest version!		 Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OMu04854 NM_001111277.2
Latest version!		 Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OMu21214 NM_175135.5
Latest version!		 Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu33274 XM_006502649.2
Latest version!		 Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OMu04854 NM_001111277.1 Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OMu33274 XM_006502649.1 Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OMu21214 NM_175135.4 Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu33275
Clone ID Related Accession (Same CDS sequence) NM_001355225.1 , XM_006502650.1
Accession Version NM_001355225.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-25
Organism Mus musculus(house mouse)
Product translation initiation factor eIF-2B subunit gamma isoform 3
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ828648.1, BY277686.1, AK145856.1, DV041891.1, BM945817.1, BQ176818.1 and BE952243.1. On Sep 13, 2017 this sequence version replaced XM_006502650.1. ##Evidence-Data-START## Transcript exon combination :: AK172173.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
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181
241
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361
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481
541
601
661
ATGGAGAATA GGTCAATCAC TTCTATCCGA AGCGAACTGA TTCCATACCT AGTAAGAAAA 
CAGTTTTCCT CAGCTTCCTC ACAGCAAAGG CAGGAAGACA AAGAAGAGGA TCTAAAGAAA 
AAGGAGCTGA AGTCTTTAGA TATTTACAGT TTTATAAAAA AAGACGACAC TCTGACCTTG 
GCTCCCTATG ATGCCTGCTG GAATGCTTTT CGAGGAGACA AATGGGAAGA TTTGTCTAGA 
TCACAGGTCC GCTGCTATGT CCACATCATG AAAGAAGGAC TTTGCTCTCG AGTAAGCACA 
CTGGGACTCT ACATGGAAGC CAACAGACAG GTCCCCAAAT TGCTATCTGT TCTTTGTCCG 
GAGGAATCAA TGATCCATTC ATCAGCCCAG ATTGTCAACA AACACCTGAT TGGAGCTGAT 
AGCCTCATCG GGCCAGATAC ACAAATTGGA GAGAAGTCAT CTATTAAGCA CTCTGTCATT 
GGCTCATCCT GTGTCATACG AGACAGAACG TCTATTACCA ATTGCCTCCT CATGAACTCT 
GTCACCGTGG AAGAAGGGAG CAGCATCCAC GGCAGTGTCA TCTGCAACAA CGCAGTGATC 
GAGACGGGCG CAGAGATCAA GAACTGTTTG GTTGGAAGTG GTCAGAGGAT TGAAGCCAAA 
GCTAAACGAA TGAACGAGGT GATCGTGGGG AATGACCAGC TCATGGAAAT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001342154.1
CDS619..1332
Translation

Target ORF information:

RefSeq Version NM_001355225.1
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001355225.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGAATA GGTCAATCAC TTCTATCCGA AGCGAACTGA TTCCATACCT AGTAAGAAAA 
CAGTTTTCCT CAGCTTCCTC ACAGCAAAGG CAGGAAGACA AAGAAGAGGA TCTAAAGAAA 
AAGGAGCTGA AGTCTTTAGA TATTTACAGT TTTATAAAAA AAGACGACAC TCTGACCTTG 
GCTCCCTATG ATGCCTGCTG GAATGCTTTT CGAGGAGACA AATGGGAAGA TTTGTCTAGA 
TCACAGGTCC GCTGCTATGT CCACATCATG AAAGAAGGAC TTTGCTCTCG AGTAAGCACA 
CTGGGACTCT ACATGGAAGC CAACAGACAG GTCCCCAAAT TGCTATCTGT TCTTTGTCCG 
GAGGAATCAA TGATCCATTC ATCAGCCCAG ATTGTCAACA AACACCTGAT TGGAGCTGAT 
AGCCTCATCG GGCCAGATAC ACAAATTGGA GAGAAGTCAT CTATTAAGCA CTCTGTCATT 
GGCTCATCCT GTGTCATACG AGACAGAACG TCTATTACCA ATTGCCTCCT CATGAACTCT 
GTCACCGTGG AAGAAGGGAG CAGCATCCAC GGCAGTGTCA TCTGCAACAA CGCAGTGATC 
GAGACGGGCG CAGAGATCAA GAACTGTTTG GTTGGAAGTG GTCAGAGGAT TGAAGCCAAA 
GCTAAACGAA TGAACGAGGT GATCGTGGGG AATGACCAGC TCATGGAAAT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu33275
Clone ID Related Accession (Same CDS sequence) NM_001355225.1 , XM_006502650.1
Accession Version XM_006502650.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 714bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(House mouse)
Product translation initiation factor eIF-2B subunit gamma isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
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121
181
241
301
361
421
481
541
601
661
ATGGAGAATA GGTCAATCAC TTCTATCCGA AGCGAACTGA TTCCATACCT AGTAAGAAAA 
CAGTTTTCCT CAGCTTCCTC ACAGCAAAGG CAGGAAGACA AAGAAGAGGA TCTAAAGAAA 
AAGGAGCTGA AGTCTTTAGA TATTTACAGT TTTATAAAAA AAGACGACAC TCTGACCTTG 
GCTCCCTATG ATGCCTGCTG GAATGCTTTT CGAGGAGACA AATGGGAAGA TTTGTCTAGA 
TCACAGGTCC GCTGCTATGT CCACATCATG AAAGAAGGAC TTTGCTCTCG AGTAAGCACA 
CTGGGACTCT ACATGGAAGC CAACAGACAG GTCCCCAAAT TGCTATCTGT TCTTTGTCCG 
GAGGAATCAA TGATCCATTC ATCAGCCCAG ATTGTCAACA AACACCTGAT TGGAGCTGAT 
AGCCTCATCG GGCCAGATAC ACAAATTGGA GAGAAGTCAT CTATTAAGCA CTCTGTCATT 
GGCTCATCCT GTGTCATACG AGACAGAACG TCTATTACCA ATTGCCTCCT CATGAACTCT 
GTCACCGTGG AAGAAGGGAG CAGCATCCAC GGCAGTGTCA TCTGCAACAA CGCAGTGATC 
GAGACGGGCG CAGAGATCAA GAACTGTTTG GTTGGAAGTG GTCAGAGGAT TGAAGCCAAA 
GCTAAACGAA TGAACGAGGT GATCGTGGGG AATGACCAGC TCATGGAAAT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006502713.1
CDS600..1313
Translation

Target ORF information:

RefSeq Version XM_006502650.1
Organism Mus musculus(House mouse)
Definition Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006502650.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGAGAATA GGTCAATCAC TTCTATCCGA AGCGAACTGA TTCCATACCT AGTAAGAAAA 
CAGTTTTCCT CAGCTTCCTC ACAGCAAAGG CAGGAAGACA AAGAAGAGGA TCTAAAGAAA 
AAGGAGCTGA AGTCTTTAGA TATTTACAGT TTTATAAAAA AAGACGACAC TCTGACCTTG 
GCTCCCTATG ATGCCTGCTG GAATGCTTTT CGAGGAGACA AATGGGAAGA TTTGTCTAGA 
TCACAGGTCC GCTGCTATGT CCACATCATG AAAGAAGGAC TTTGCTCTCG AGTAAGCACA 
CTGGGACTCT ACATGGAAGC CAACAGACAG GTCCCCAAAT TGCTATCTGT TCTTTGTCCG 
GAGGAATCAA TGATCCATTC ATCAGCCCAG ATTGTCAACA AACACCTGAT TGGAGCTGAT 
AGCCTCATCG GGCCAGATAC ACAAATTGGA GAGAAGTCAT CTATTAAGCA CTCTGTCATT 
GGCTCATCCT GTGTCATACG AGACAGAACG TCTATTACCA ATTGCCTCCT CATGAACTCT 
GTCACCGTGG AAGAAGGGAG CAGCATCCAC GGCAGTGTCA TCTGCAACAA CGCAGTGATC 
GAGACGGGCG CAGAGATCAA GAACTGTTTG GTTGGAAGTG GTCAGAGGAT TGAAGCCAAA 
GCTAAACGAA TGAACGAGGT GATCGTGGGG AATGACCAGC TCATGGAAAT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu04854
Clone ID Related Accession (Same CDS sequence) NM_001111277.1 , NM_001111277.2
Accession Version NM_001111277.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-02-14
Organism Mus musculus(House mouse)
Product translation initiation factor eIF-2B subunit gamma isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY277686.1, AK145856.1, BC115657.1 and BE952243.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGAGCAGCA TCCACGGCAG TGTCATCTGC AACAACGCAG TGATCGAGAC GGGCGCAGAG 
ATCAAGAACT GTTTGGTTGG AAGTGGTCAG AGGATTGAAG CCAAAGCTAA ACGAATGAAC 
GAGGTGATCG TGGGGAATGA CCAGCTCATG GAAATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001104747.1
CDS80..1438
Translation

Target ORF information:

RefSeq Version NM_001111277.1
Organism Mus musculus(House mouse)
Definition Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001111277.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGAGCAGCA TCCACGGCAG TGTCATCTGC AACAACGCAG TGATCGAGAC GGGCGCAGAG 
ATCAAGAACT GTTTGGTTGG AAGTGGTCAG AGGATTGAAG CCAAAGCTAA ACGAATGAAC 
GAGGTGATCG TGGGGAATGA CCAGCTCATG GAAATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu04854
Clone ID Related Accession (Same CDS sequence) NM_001111277.1 , NM_001111277.2
Accession Version NM_001111277.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-25
Organism Mus musculus(house mouse)
Product translation initiation factor eIF-2B subunit gamma isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ828648.1, BY277686.1, AK145856.1, BC115657.1 and BE952243.1. On Sep 20, 2017 this sequence version replaced NM_001111277.1. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.127166.1, SRR1660819.306631.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGAGCAGCA TCCACGGCAG TGTCATCTGC AACAACGCAG TGATCGAGAC GGGCGCAGAG 
ATCAAGAACT GTTTGGTTGG AAGTGGTCAG AGGATTGAAG CCAAAGCTAA ACGAATGAAC 
GAGGTGATCG TGGGGAATGA CCAGCTCATG GAAATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001104747.1
CDS86..1444
Translation

Target ORF information:

RefSeq Version NM_001111277.2
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001111277.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGAGCAGCA TCCACGGCAG TGTCATCTGC AACAACGCAG TGATCGAGAC GGGCGCAGAG 
ATCAAGAACT GTTTGGTTGG AAGTGGTCAG AGGATTGAAG CCAAAGCTAA ACGAATGAAC 
GAGGTGATCG TGGGGAATGA CCAGCTCATG GAAATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu21214
Clone ID Related Accession (Same CDS sequence) NM_175135.5 , NM_175135.4
Accession Version NM_175135.5 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2018-05-25
Organism Mus musculus(house mouse)
Product translation initiation factor eIF-2B subunit gamma isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ828648.1, BY277686.1, AK145856.1 and AK165157.1. On Sep 13, 2017 this sequence version replaced NM_175135.4. ##Evidence-Data-START## Transcript exon combination :: AK145856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGTATGTCT CCCTCTGTGC CTTTTTGAGC AAGGCTTCTG GTAGCCCTAG GAGCCTTATC 
AGTCCTACCC AGAAGAGTTT GCTTTGGGAC AGGAAGGAAG GGAAGGGAAG GAAGAAGGTT 
GGTGCCTTTG GAGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_780344.2
CDS86..1423
Translation

Target ORF information:

RefSeq Version NM_175135.5
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_175135.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGTATGTCT CCCTCTGTGC CTTTTTGAGC AAGGCTTCTG GTAGCCCTAG GAGCCTTATC 
AGTCCTACCC AGAAGAGTTT GCTTTGGGAC AGGAAGGAAG GGAAGGGAAG GAAGAAGGTT 
GGTGCCTTTG GAGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu21214
Clone ID Related Accession (Same CDS sequence) NM_175135.5 , NM_175135.4
Accession Version NM_175135.4 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-02-14
Organism Mus musculus(House mouse)
Product translation initiation factor eIF-2B subunit gamma isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY277686.1, AK145856.1 and AK165157.1. On Dec 11, 2007 this sequence version replaced NM_175135.3. ##Evidence-Data-START## Transcript exon combination :: AK145856.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGTATGTCT CCCTCTGTGC CTTTTTGAGC AAGGCTTCTG GTAGCCCTAG GAGCCTTATC 
AGTCCTACCC AGAAGAGTTT GCTTTGGGAC AGGAAGGAAG GGAAGGGAAG GAAGAAGGTT 
GGTGCCTTTG GAGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_780344.2
CDS80..1417
Translation

Target ORF information:

RefSeq Version NM_175135.4
Organism Mus musculus(House mouse)
Definition Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_175135.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGTATGTCT CCCTCTGTGC CTTTTTGAGC AAGGCTTCTG GTAGCCCTAG GAGCCTTATC 
AGTCCTACCC AGAAGAGTTT GCTTTGGGAC AGGAAGGAAG GGAAGGGAAG GAAGAAGGTT 
GGTGCCTTTG GAGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu33274
Clone ID Related Accession (Same CDS sequence) XM_006502649.1 , XM_006502649.2
Accession Version XM_006502649.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product translation initiation factor eIF-2B subunit gamma isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGTGACATGA ACTCAGAATA TCACTTCTAC CCTCCCACAC CAGAACACAG TGCTCCTGTG 
AGAAACTGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006502712.1
CDS89..1360
Translation

Target ORF information:

RefSeq Version XM_006502649.1
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006502649.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGTGACATGA ACTCAGAATA TCACTTCTAC CCTCCCACAC CAGAACACAG TGCTCCTGTG 
AGAAACTGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu33274
Clone ID Related Accession (Same CDS sequence) XM_006502649.1 , XM_006502649.2
Accession Version XM_006502649.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product translation initiation factor eIF-2B subunit gamma isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGTGACATGA ACTCAGAATA TCACTTCTAC CCTCCCACAC CAGAACACAG TGCTCCTGTG 
AGAAACTGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006502712.1
CDS89..1360
Translation

Target ORF information:

RefSeq Version XM_006502649.2
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic translation initiation factor 2B, subunit 3 (Eif2b3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006502649.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGAGTTTC AAGCGGTGGT TATGGCCGTT GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGTGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTCT GCGCAGAATT CAAGATGAAG ATGAAGCTAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGACACATCT ATCCAAAGCT TAAGACCGAT 
GTGCTGGTCC TGAGCTGCGA TCTGATAACA GATGTGGCTT TACATGAGGT TGTGGACCTG 
TTCAGAGCTT ATGATGCGTC ACTGGCCATG CTAATGAGGA AAGGACAAGA GAGCATAGAA 
CCAGTTCCAG GTCAAAAGGG GAAAAAAAAA CCAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCACAC AGGCCTTGTG 
GATGCCCATC TCTACTGCCT GAAGAAGTAC GTTGTGGATT TCTTAATGGA GAATAGGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCT 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCTGAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC GACACTCTGA CCTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAGG AGACAAATGG GAAGATTTGT CTAGATCACA GGTCCGCTGC 
TATGTCCACA TCATGAAAGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTCCC CAAATTGCTA TCTGTTCTTT GTCCGGAGGA ATCAATGATC 
CATTCATCAG CCCAGATTGT CAACAAACAC CTGATTGGAG CTGATAGCCT CATCGGGCCA 
GATACACAAA TTGGAGAGAA GTCATCTATT AAGCACTCTG TCATTGGCTC ATCCTGTGTC 
ATACGAGACA GAACGTCTAT TACCAATTGC CTCCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGTGACATGA ACTCAGAATA TCACTTCTAC CCTCCCACAC CAGAACACAG TGCTCCTGTG 
AGAAACTGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA


book

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
PLoS biology9(1)e1000582(2011 Jan)
Diez-Roux G,Banfi S,Sultan M,Geffers L,Anand S,Rozado D,Magen A,Canidio E,Pagani M,Peluso I,Lin-Marq N,Koch M,Bilio M,Cantiello I,Verde R,De Masi C,Bianchi SA,Cicchini J,Perroud E,Mehmeti S,Dagand E,Schrinner S,N?rnberger A,Schmidt K,Metz K,Zwingmann C,Brieske N,Springer C,Hernandez AM,Herzog S,Grabbe F,Sieverding C,Fischer B,Schrader K,Brockmeyer M,Dettmer S,Helbig C,Alunni V,Battaini MA,Mura C,Henrichsen CN,Garcia-Lopez R,Echevarria D,Puelles E,Garcia-Calero E,Kruse S,Uhr M,Kauck C,Feng G,Milyaev N,Ong CK,Kumar L,Lam M,Semple CA,Gyenesei A,Mundlos S,Radelof U,Lehrach H,Sarmientos P,Reymond A,Davidson DR,Doll? P,Antonarakis SE,Yaspo ML,Martinez S,Baldock RA,Eichele G,Ballabio A


book

Large-scale gene trapping in C57BL/6N mouse embryonic stem cells.
Genome research18(10)1670-9(2008 Oct)
Hansen GM,Markesich DC,Burnett MB,Zhu Q,Dionne KM,Richter LJ,Finnell RH,Sands AT,Zambrowicz BP,Abuin A


book

EUCOMM--the European conditional mouse mutagenesis program.
Briefings in functional genomics & proteomics6(3)180-5(2007 Sep)
Friedel RH,Seisenberger C,Kaloff C,Wurst W


book

BGEM: an in situ hybridization database of gene expression in the embryonic and adult mouse nervous system.
PLoS biology4(4)e86(2006 Apr)
Magdaleno S,Jensen P,Brumwell CL,Seal A,Lehman K,Asbury A,Cheung T,Cornelius T,Batten DM,Eden C,Norland SM,Rice DS,Dosooye N,Shakya S,Mehta P,Curran T