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Eif2b3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Eif2b3
Entrez Gene ID 171145
Full Name eukaryotic translation initiation factor 2B subunit gamma
General protein information
Preferred Names

eukaryotic translation initiation factor 2B subunit gamma

Names

translation initiation factor eIF-2B subunit gamma
eIF-2B GDP-GTP exchange factor subunit gamma
eukaryotic translation initiation factor 2B, subunit 3 (gamma, 58kD)
eukaryotic translation initiation factor 2B, subunit 3 gamma
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

5

5q36
Summary guanine nucleotide exchange factor that regulates key step in protein synthesis; restores eIF2 to its active GTP-bound state [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_133609.2 NP_598293.2 translation initiation factor eIF-2B subunit gamma
XM_006238630.3 XP_006238692.1 translation initiation factor eIF-2B subunit gamma isoform X1

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) EIF2B3 XP_001152272.1
EIF2B3 XP_001094199.2
Bos taurus (cattle) EIF2B3 NP_001076943.1
Gallus gallus (chicken) EIF2B3 NP_001026451.1
Drosophila melanogaster (fruit fly) eIF2B-gamma NP_611046.2
Neurospora crassa NCU03548 XP_955772.2
Arabidopsis thaliana (thale cress) AT5G19485 NP_001031908.1
Homo sapiens (human) EIF2B3 NP_065098.1
Caenorhabditis elegans (roundworm) ppp-1 NP_495428.1
Schizosaccharomyces pombe (fission yeast) tif223 NP_594962.2
Canis lupus familiaris (dog) EIF2B3 XP_005629136.1
Mus musculus (house mouse) Eif2b3 NP_001104747.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP005210 XP_314112.3
Rattus norvegicus (Norway rat) Eif2b3 NP_598293.2
Danio rerio (zebrafish) eif2b3 NP_957368.1
Magnaporthe oryzae (rice blast fungus) MGG_02390 XP_003709167.1
Oryza sativa (rice) Os06g0338900 NP_001057561.1
Xenopus tropicalis (tropical clawed frog) eif2b3 NP_001016997.2

Gene Ontology


Bibliography

Related articles in PubMed

The mechanism of eukaryotic translation initiation: new insights and challenges.
Hinnebusch AG, Lorsch JR
Cold Spring Harbor perspectives in biology4(10)(2012 Oct)

An efficient mammalian cell-free translation system supplemented with translation factors.
Mikami S, Masutani M, Sonenberg N, Yokoyama S, Imataka H
Protein expression and purification46(2)348-57(2006 Apr)

Decreased guanine nucleotide exchange factor activity in eIF2B-mutated patients.
Fogli A, Schiffmann R, Hugendubler L, Combes P, Bertini E, Rodriguez D, Kimball SR, Boespflug-Tanguy O
European journal of human genetics : EJHG12(7)561-6(2004 Jul)

The life and death of oligodendrocytes in vanishing white matter disease.
Van Haren K, van der Voorn JP, Peterson DR, van der Knaap MS, Powers JM
Journal of neuropathology and experimental neurology63(6)618-30(2004 Jun)

Mutations linked to leukoencephalopathy with vanishing white matter impair the function of the eukaryotic initiation factor 2B complex in diverse ways.
Li W, Wang X, Van Der Knaap MS, Proud CG
Molecular and cellular biology24(8)3295-306(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Eif2b3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2b3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa01064 NM_133609.2
Latest version!		 Rattus norvegicus eukaryotic translation initiation factor 2B subunit gamma (Eif2b3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
ORa01064 XM_006238630.3
Latest version!		 Rattus norvegicus eukaryotic translation initiation factor 2B, subunit 3 gamma (Eif2b3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa01064
Clone ID Related Accession (Same CDS sequence) NM_133609.2 , XM_006238630.3
Accession Version NM_133609.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-19
Organism Rattus norvegicus(Norway rat)
Product translation initiation factor eIF-2B subunit gamma
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC072507.1. On Jun 13, 2004 this sequence version replaced NM_133609.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC072507.1, U38253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCCGTAGT TATGGCCGTC GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGAGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTGT GTGCAGAGTT CAAGATGAAA TTGAAGCCAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGCCACATAT ATCCAAAACT TAAGACTGAT 
GTGCTGGTCC TGGGCTGCGA TCTGATAACA GATGTTGCCT TACACGAGGT TGTGGACCTG 
TTCAGAGCTT ACGATGCATC ACTTGCCATG TTAATGAGGA AAGGACAAGA GAGCACAGAA 
CCAGTTCCAG GTCAGAAGGG GAAAAAAAAA ACAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCAGAC AGGCCTTGTG 
GATGCTCATC TCTACTGCTT GAAGAAATAT GTTGTGGATT TCTTAATGGA AAATAAGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCC 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCCCAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC AACACTCTGA CTTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAAG AGACAAGTGG GAAGATTTGT CTAGATCACA GGTTCGCTGC 
TATGTCCACA TCATGAAGGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTACC CAAATTGCTG TCTGTTCTTT GTCCTGAGGA ATCAATGATC 
CATCCATCAG CCCAGATTGC CAACAAACAC CTGATTGGAG CCGACAGCCT TATCGGGTCA 
GATACACAAG TTGGAGAGAA GTCATCTATT AAACGATCTG TCATTGGCTC ATCCTGTGTC 
ATCAGAGACA GAGTAACTGT TACCAACTGC CTTCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGAGCAGCA TCCATGGCAG CGTCATCTGC AACAACGCCG TGGTCGAGGC GGGCGCGGAG 
ATCAGGGACT GTTTAATTGG AAGTGGTCAG AGGATTGAAG CCAAAGCTAA ACGCATGAAC 
GAGGTGATCG TGGGAAATGA CCAGCTCATG GAGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_598293.2
CDS109..1467
Translation

Target ORF information:

RefSeq Version NM_133609.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus eukaryotic translation initiation factor 2B subunit gamma (Eif2b3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_133609.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCCGTAGT TATGGCCGTC GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGAGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTGT GTGCAGAGTT CAAGATGAAA TTGAAGCCAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGCCACATAT ATCCAAAACT TAAGACTGAT 
GTGCTGGTCC TGGGCTGCGA TCTGATAACA GATGTTGCCT TACACGAGGT TGTGGACCTG 
TTCAGAGCTT ACGATGCATC ACTTGCCATG TTAATGAGGA AAGGACAAGA GAGCACAGAA 
CCAGTTCCAG GTCAGAAGGG GAAAAAAAAA ACAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCAGAC AGGCCTTGTG 
GATGCTCATC TCTACTGCTT GAAGAAATAT GTTGTGGATT TCTTAATGGA AAATAAGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCC 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCCCAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC AACACTCTGA CTTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAAG AGACAAGTGG GAAGATTTGT CTAGATCACA GGTTCGCTGC 
TATGTCCACA TCATGAAGGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTACC CAAATTGCTG TCTGTTCTTT GTCCTGAGGA ATCAATGATC 
CATCCATCAG CCCAGATTGC CAACAAACAC CTGATTGGAG CCGACAGCCT TATCGGGTCA 
GATACACAAG TTGGAGAGAA GTCATCTATT AAACGATCTG TCATTGGCTC ATCCTGTGTC 
ATCAGAGACA GAGTAACTGT TACCAACTGC CTTCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGAGCAGCA TCCATGGCAG CGTCATCTGC AACAACGCCG TGGTCGAGGC GGGCGCGGAG 
ATCAGGGACT GTTTAATTGG AAGTGGTCAG AGGATTGAAG CCAAAGCTAA ACGCATGAAC 
GAGGTGATCG TGGGAAATGA CCAGCTCATG GAGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa01064
Clone ID Related Accession (Same CDS sequence) NM_133609.2 , XM_006238630.3
Accession Version XM_006238630.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1359bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product translation initiation factor eIF-2B subunit gamma isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006238630.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCCGTAGT TATGGCCGTC GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGAGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTGT GTGCAGAGTT CAAGATGAAA TTGAAGCCAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGCCACATAT ATCCAAAACT TAAGACTGAT 
GTGCTGGTCC TGGGCTGCGA TCTGATAACA GATGTTGCCT TACACGAGGT TGTGGACCTG 
TTCAGAGCTT ACGATGCATC ACTTGCCATG TTAATGAGGA AAGGACAAGA GAGCACAGAA 
CCAGTTCCAG GTCAGAAGGG GAAAAAAAAA ACAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCAGAC AGGCCTTGTG 
GATGCTCATC TCTACTGCTT GAAGAAATAT GTTGTGGATT TCTTAATGGA AAATAAGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCC 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCCCAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC AACACTCTGA CTTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAAG AGACAAGTGG GAAGATTTGT CTAGATCACA GGTTCGCTGC 
TATGTCCACA TCATGAAGGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTACC CAAATTGCTG TCTGTTCTTT GTCCTGAGGA ATCAATGATC 
CATCCATCAG CCCAGATTGC CAACAAACAC CTGATTGGAG CCGACAGCCT TATCGGGTCA 
GATACACAAG TTGGAGAGAA GTCATCTATT AAACGATCTG TCATTGGCTC ATCCTGTGTC 
ATCAGAGACA GAGTAACTGT TACCAACTGC CTTCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGAGCAGCA TCCATGGCAG CGTCATCTGC AACAACGCCG TGGTCGAGGC GGGCGCGGAG 
ATCAGGGACT GTTTAATTGG AAGTGGTCAG AGGATTGAAG CCAAAGCTAA ACGCATGAAC 
GAGGTGATCG TGGGAAATGA CCAGCTCATG GAGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006238692.1
CDS89..1447
Translation

Target ORF information:

RefSeq Version XM_006238630.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus eukaryotic translation initiation factor 2B, subunit 3 gamma (Eif2b3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006238630.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGAGTTTC AAGCCGTAGT TATGGCCGTC GGTGGGGGAT CTCGGATGAC AGACTTGACT 
TCCAGCATTC CCAAGCCTCT GCTTCCAGTT GGAAACAAAC CTTTAATTTG GTACCCGCTG 
AACCTGCTTG AGCGAGTTGG ATTTGAAGAA GTCATCGTGG TTACAACCAA AGATGTCCAA 
AAGGCTCTGT GTGCAGAGTT CAAGATGAAA TTGAAGCCAG ATATTGTGTG CATTCCTGAT 
GAGGCAGACA TGGGGACTGC AGATTCTCTA CGCCACATAT ATCCAAAACT TAAGACTGAT 
GTGCTGGTCC TGGGCTGCGA TCTGATAACA GATGTTGCCT TACACGAGGT TGTGGACCTG 
TTCAGAGCTT ACGATGCATC ACTTGCCATG TTAATGAGGA AAGGACAAGA GAGCACAGAA 
CCAGTTCCAG GTCAGAAGGG GAAAAAAAAA ACAGTGGAGC AGCGTGACTT CATTGGAGTG 
GACAGCACAG GAAAAAGGCT GCTCTTTATG GCTAATGAAG CAGACTTGGA TGAAGAGCTG 
GTCATTAAGG GATCCATCCT GCAGAAGCAC CCTAGGATAC ATTTCCAGAC AGGCCTTGTG 
GATGCTCATC TCTACTGCTT GAAGAAATAT GTTGTGGATT TCTTAATGGA AAATAAGTCA 
ATCACTTCTA TCCGAAGCGA ACTGATTCCA TACCTAGTAA GAAAACAGTT TTCCTCAGCC 
TCCTCACAGC AAAGGCAGGA AGACAAAGAA GAGGATCTAA AGAAAAAGGA GCCCAAGTCT 
TTAGATATTT ACAGTTTTAT AAAAAAAGAC AACACTCTGA CTTTGGCTCC CTATGATGCC 
TGCTGGAATG CTTTTCGAAG AGACAAGTGG GAAGATTTGT CTAGATCACA GGTTCGCTGC 
TATGTCCACA TCATGAAGGA AGGACTTTGC TCTCGAGTAA GCACACTGGG ACTCTACATG 
GAAGCCAACA GACAGGTACC CAAATTGCTG TCTGTTCTTT GTCCTGAGGA ATCAATGATC 
CATCCATCAG CCCAGATTGC CAACAAACAC CTGATTGGAG CCGACAGCCT TATCGGGTCA 
GATACACAAG TTGGAGAGAA GTCATCTATT AAACGATCTG TCATTGGCTC ATCCTGTGTC 
ATCAGAGACA GAGTAACTGT TACCAACTGC CTTCTCATGA ACTCTGTCAC CGTGGAAGAA 
GGGAGCAGCA TCCATGGCAG CGTCATCTGC AACAACGCCG TGGTCGAGGC GGGCGCGGAG 
ATCAGGGACT GTTTAATTGG AAGTGGTCAG AGGATTGAAG CCAAAGCTAA ACGCATGAAC 
GAGGTGATCG TGGGAAATGA CCAGCTCATG GAGATCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The mechanism of eukaryotic translation initiation: new insights and challenges.
Cold Spring Harbor perspectives in biology4(10)(2012 Oct)
Hinnebusch AG,Lorsch JR


book

An efficient mammalian cell-free translation system supplemented with translation factors.
Protein expression and purification46(2)348-57(2006 Apr)
Mikami S,Masutani M,Sonenberg N,Yokoyama S,Imataka H


book

Decreased guanine nucleotide exchange factor activity in eIF2B-mutated patients.
European journal of human genetics : EJHG12(7)561-6(2004 Jul)
Fogli A,Schiffmann R,Hugendubler L,Combes P,Bertini E,Rodriguez D,Kimball SR,Boespflug-Tanguy O


book

The life and death of oligodendrocytes in vanishing white matter disease.
Journal of neuropathology and experimental neurology63(6)618-30(2004 Jun)
Van Haren K,van der Voorn JP,Peterson DR,van der Knaap MS,Powers JM


book

Mutations linked to leukoencephalopathy with vanishing white matter impair the function of the eukaryotic initiation factor 2B complex in diverse ways.
Molecular and cellular biology24(8)3295-306(2004 Apr)
Li W,Wang X,Van Der Knaap MS,Proud CG