Nthl1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nthl1
Entrez Gene ID	18207
Full Name	nth (endonuclease III)-like 1 (E.coli)
Synonyms	Nth1,Octs3
General protein information	Preferred Names nth (endonuclease III)-like 1 (E.coli) Names endonuclease III-like protein 1 DNA glycoslyase/AP lyase DNA glycosylase/AP lyase bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase thymine glycol DNA glycosylase/AP lyase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 A3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_008743.2	NP_032769.2	endonuclease III-like protein 1 isoform 1
XM_006523819.3	XP_006523882.1	endonuclease III-like protein 1 isoform X1
NM_001357615.1	NP_001344544.1	endonuclease III-like protein 1 isoform 2
XM_006523819.2	XP_006523882.1	endonuclease III-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NTHL1	XP_853674.1
Xenopus tropicalis (tropical clawed frog)	LOC100488928	XP_004918108.1
Homo sapiens (human)	NTHL1	NP_002519.1
	NTHL1	XP_001082772.1
Drosophila melanogaster (fruit fly)	CG9272	NP_610078.2
Magnaporthe oryzae (rice blast fungus)	MGG_06014	XP_003711861.1
Xenopus tropicalis (tropical clawed frog)	nthl1	NP_001037884.1
Bos taurus (cattle)	NTHL1	NP_001039862.1
Rattus norvegicus (Norway rat)	Nthl1	NP_001099198.1
Gallus gallus (chicken)	NTHL1	NP_001073043.1
Danio rerio (zebrafish)	nthl1	XP_001346643.3
Mus musculus (house mouse)	Nthl1	NP_032769.2
Caenorhabditis elegans (roundworm)	nth-1	NP_001254907.1
Schizosaccharomyces pombe (fission yeast)	nth1	NP_593210.1
Neurospora crassa	NCU06654	XP_960699.2

Gene Ontology
Bibliography

Related articles in PubMed

Defective repair of oxidative base lesions by the DNA glycosylase Nth1 associates with multiple telomere defects.
Vallabhaneni H, O'Callaghan N, Sidorova J, Liu Y
PLoS genetics9(7)e1003639(2013)

The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells.
Cooley N, Elder RH, Povey AC
BioMed research international2013152909(2013)

The DNA glycosylases Ogg1 and Nth1 do not contribute to Ig class switching in activated mouse splenic B cells.
Ucher AJ, Linehan EK, Teebor GW, Schrader CE, Stavnezer J
PloS one7(4)e36061(2012)

Targeted deletion of the genes encoding NTH1 and NEIL1 DNA N-glycosylases reveals the existence of novel carcinogenic oxidative damage to DNA.
Chan MK, Ocampo-Hafalla MT, Vartanian V, Jaruga P, Kirkali G, Koenig KL, Brown S, Lloyd RS, Dizdaroglu M, Teebor GW
DNA repair8(7)786-94(2009 Jul)

OGG1 initiates age-dependent CAG trinucleotide expansion in somatic cells.
Kovtun IV, Liu Y, Bjoras M, Klungland A, Wilson SH, McMurray CT
Nature447(7143)447-52(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that Nth1 plays an important role in telomere maintenance and base repair against oxidative stress-induced base modifications.
Title: Defective repair of oxidative base lesions by the DNA glycosylase Nth1 associates with multiple telomere defects.

Toxicity, induced by tert-butyl-hydroperoxide and potassium bromate, differs in base excision repair proficient (Mpg (+/+), Nth1 (+/+)) and deficient (Mpg (-/-), Nth1 (-/-)) mouse embryonic fibroblasts following Msh2 knockdown, was examined.
Title: The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells.

deficiencies in Ogg1 and/or Nth1 do not lead to decreased DNA double strand breaks
Title: The DNA glycosylases Ogg1 and Nth1 do not contribute to Ig class switching in activated mouse splenic B cells.

The high incidence of tumors in Nth1-/-Neil1-/- mice together with the activating mutation in the K-ras gene of their pulmonary tumors, reveal for the first time, the existence of mutagenic and carcinogenic oxidative damage to DNA which is not 8-OH-Gua.
Title: Targeted deletion of the genes encoding NTH1 and NEIL1 DNA N-glycosylases reveals the existence of novel carcinogenic oxidative damage to DNA.

results indicated that 8-oxoguanine DNA-glycosylase (OGG1) and nth (endonuclease III)-like 1(NTH1) are the major DNA glycosylases for the removal of 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 4,6-diamino-5-formamidopyrimidine (FapyA)
Title: Repair of formamidopyrimidines in DNA involves different glycosylases: role of the OGG1, NTH1, and NEIL1 enzymes.

The following Nthl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nthl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18065	NM_008743.2 Latest version!	Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu53269	NM_001357615.1 Latest version!	Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu53269	XM_006523819.3 Latest version!	Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu53269	XM_006523819.2	Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nthl1 ( NM_008743.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032769.2 Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18065
Clone ID Related Accession (Same CDS sequence)	NM_008743.2
Accession Version	NM_008743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	endonuclease III-like protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132367.3, CX565655.1, AV377313.2 and CJ211561.1. On Apr 28, 2009 this sequence version replaced NM_008743.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y09688.1, AB006812.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTCAG GGGTGCGGAT GGTGACTCGC AGTCGGAGCC GCGCGACTAG GATCGCGTCG 
GAAGGGTGTA GGGAGGAGCT CGCCCCGCGA GAGGCTGCTG CAGAAGGAAG AAAAAGCCAC 
AGGCCCGTGA GACATCCACG GAGAACACAG AAAACGCATG TGGCCTATGA AGCGGCTAAT 
GGTGAGGAAG GCGAAGATGC TGAGCCCCTC AAAGTGCCCG TTTGGGAGCC CCAGAACTGG 
CAGCAGCAAC TGGCCAACAT CCGCATCATG AGAAGCAAGA AGGATGCACC TGTGGACCAG 
CTAGGCGCCG AGCACTGCTA TGATGCAAGT GCCTCCCCGA AGGTGAGGAG GTACCAGGTA 
CTCCTGTCGC TGATGCTCTC CAGCCAGACC AAAGACCAGG TCACAGCAGG TGCTATGCAA 
CGGCTCCGGG CCCGGGGCTT GACTGTGGAG AGCATCCTGC AGACCGATGA TGACACGCTA 
GGCAGACTCA TCTACCCTGT GGGCTTCTGG AGGAACAAGG TAAAATACAT CAAGCAGACA 
ACCGCCATCC TGCAGCAGCG CTACGAAGGG GACATCCCTG CTTCCGTGGC TGAGCTGGTA 
GCCTTGCCAG GTGTTGGGCC CAAGATGGCA CACTTGGCTA TGGCTGTGGC CTGGGGGACC 
ATATCAGGCA TAGCAGTGGA CACACATGTG CACAGAATAG CCAACCGACT GAGGTGGACC 
AAGAAGATGA CCAAGACCCC AGAAGAGACA CGTAAGAACT TGGAAGAGTG GCTACCCAGG 
GTGCTGTGGA GTGAGGTCAA CGGACTACTG GTAGGCTTCG GCCAACAGAT CTGTCTTCCT 
GTCCATCCTC GATGTCAGGC TTGCCTCAAC AAGGCCCTGT GCCCTGCTGC CCAGGATCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032769.2
CDS	71..973
Translation	MNSGVRMVTRSRSRATRIASEGCREELAPREAAAEGRKSHRPVRHPRRTQKTHVAYEAANGEEGEDAEPLKVPVWEPQNWQQQLANIRIMRSKKDAPVDQLGAEHCYDASASPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVESILQTDDDTLGRLIYPVGFWRNKVKYIKQTTAILQQRYEGDIPASVAELVALPGVGPKMAHLAMAVAWGTISGIAVDTHVHRIANRLRWTKKMTKTPEETRKNLEEWLPRVLWSEVNGLLVGFGQQICLPVHPRCQACLNKALCPAAQDL

Target ORF information:

RefSeq Version	NM_008743.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008743.2

ORF Insert Sequence:

ATGAACTCAG GGGTGCGGAT GGTGACTCGC AGTCGGAGCC GCGCGACTAG GATCGCGTCG 
GAAGGGTGTA GGGAGGAGCT CGCCCCGCGA GAGGCTGCTG CAGAAGGAAG AAAAAGCCAC 
AGGCCCGTGA GACATCCACG GAGAACACAG AAAACGCATG TGGCCTATGA AGCGGCTAAT 
GGTGAGGAAG GCGAAGATGC TGAGCCCCTC AAAGTGCCCG TTTGGGAGCC CCAGAACTGG 
CAGCAGCAAC TGGCCAACAT CCGCATCATG AGAAGCAAGA AGGATGCACC TGTGGACCAG 
CTAGGCGCCG AGCACTGCTA TGATGCAAGT GCCTCCCCGA AGGTGAGGAG GTACCAGGTA 
CTCCTGTCGC TGATGCTCTC CAGCCAGACC AAAGACCAGG TCACAGCAGG TGCTATGCAA 
CGGCTCCGGG CCCGGGGCTT GACTGTGGAG AGCATCCTGC AGACCGATGA TGACACGCTA 
GGCAGACTCA TCTACCCTGT GGGCTTCTGG AGGAACAAGG TAAAATACAT CAAGCAGACA 
ACCGCCATCC TGCAGCAGCG CTACGAAGGG GACATCCCTG CTTCCGTGGC TGAGCTGGTA 
GCCTTGCCAG GTGTTGGGCC CAAGATGGCA CACTTGGCTA TGGCTGTGGC CTGGGGGACC 
ATATCAGGCA TAGCAGTGGA CACACATGTG CACAGAATAG CCAACCGACT GAGGTGGACC 
AAGAAGATGA CCAAGACCCC AGAAGAGACA CGTAAGAACT TGGAAGAGTG GCTACCCAGG 
GTGCTGTGGA GTGAGGTCAA CGGACTACTG GTAGGCTTCG GCCAACAGAT CTGTCTTCCT 
GTCCATCCTC GATGTCAGGC TTGCCTCAAC AAGGCCCTGT GCCCTGCTGC CCAGGATCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nthl1 ( NM_001357615.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344544.1 Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu53269
Clone ID Related Accession (Same CDS sequence)	XM_006523819.3 , NM_001357615.1 , XM_006523819.2
Accession Version	NM_001357615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	endonuclease III-like protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132367.3 and CX567634.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAAGCA AGAAGGATGC ACCTGTGGAC CAGCTAGGCG CCGAGCACTG CTATGATGCA 
AGTGCCTCCC CGAAGGTGAG GAGGTACCAG GTACTCCTGT CGCTGATGCT CTCCAGCCAG 
ACCAAAGACC AGGTCACAGC AGGTGCTATG CAACGGCTCC GGGCCCGGGG CTTGACTGTG 
GAGAGCATCC TGCAGACCGA TGATGACACG CTAGGCAGAC TCATCTACCC TGTGGGCTTC 
TGGAGGAACA AGGTAAAATA CATCAAGCAG ACAACCGCCA TCCTGCAGCA GCGCTACGAA 
GGGGACATCC CTGCTTCCGT GGCTGAGCTG GTAGCCTTGC CAGGTGTTGG GCCCAAGATG 
GCACACTTGG CTATGGCTGT GGCCTGGGGG ACCATATCAG GCATAGCAGT GGACACACAT 
GTGCACAGAA TAGCCAACCG ACTGAGGTGG ACCAAGAAGA TGACCAAGAC CCCAGAAGAG 
ACACGTAAGA ACTTGGAAGA GTGGCTACCC AGGGTGCTGT GGAGTGAGGT CAACGGACTA 
CTGGTAGGCT TCGGCCAACA GATCTGTCTT CCTGTCCATC CTCGATGTCA GGCTTGCCTC 
AACAAGGCCC TGTGCCCTGC TGCCCAGGAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344544.1
CDS	247..882
Translation	MRSKKDAPVDQLGAEHCYDASASPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVESILQTDDDTLGRLIYPVGFWRNKVKYIKQTTAILQQRYEGDIPASVAELVALPGVGPKMAHLAMAVAWGTISGIAVDTHVHRIANRLRWTKKMTKTPEETRKNLEEWLPRVLWSEVNGLLVGFGQQICLPVHPRCQACLNKALCPAAQDL

Target ORF information:

RefSeq Version	NM_001357615.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357615.1

ORF Insert Sequence:

ATGAGAAGCA AGAAGGATGC ACCTGTGGAC CAGCTAGGCG CCGAGCACTG CTATGATGCA 
AGTGCCTCCC CGAAGGTGAG GAGGTACCAG GTACTCCTGT CGCTGATGCT CTCCAGCCAG 
ACCAAAGACC AGGTCACAGC AGGTGCTATG CAACGGCTCC GGGCCCGGGG CTTGACTGTG 
GAGAGCATCC TGCAGACCGA TGATGACACG CTAGGCAGAC TCATCTACCC TGTGGGCTTC 
TGGAGGAACA AGGTAAAATA CATCAAGCAG ACAACCGCCA TCCTGCAGCA GCGCTACGAA 
GGGGACATCC CTGCTTCCGT GGCTGAGCTG GTAGCCTTGC CAGGTGTTGG GCCCAAGATG 
GCACACTTGG CTATGGCTGT GGCCTGGGGG ACCATATCAG GCATAGCAGT GGACACACAT 
GTGCACAGAA TAGCCAACCG ACTGAGGTGG ACCAAGAAGA TGACCAAGAC CCCAGAAGAG 
ACACGTAAGA ACTTGGAAGA GTGGCTACCC AGGGTGCTGT GGAGTGAGGT CAACGGACTA 
CTGGTAGGCT TCGGCCAACA GATCTGTCTT CCTGTCCATC CTCGATGTCA GGCTTGCCTC 
AACAAGGCCC TGTGCCCTGC TGCCCAGGAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nthl1 ( XM_006523819.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523882.1 Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53269
Clone ID Related Accession (Same CDS sequence)	XM_006523819.3 , NM_001357615.1 , XM_006523819.2
Accession Version	XM_006523819.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	endonuclease III-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006523819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGCA AGAAGGATGC ACCTGTGGAC CAGCTAGGCG CCGAGCACTG CTATGATGCA 
AGTGCCTCCC CGAAGGTGAG GAGGTACCAG GTACTCCTGT CGCTGATGCT CTCCAGCCAG 
ACCAAAGACC AGGTCACAGC AGGTGCTATG CAACGGCTCC GGGCCCGGGG CTTGACTGTG 
GAGAGCATCC TGCAGACCGA TGATGACACG CTAGGCAGAC TCATCTACCC TGTGGGCTTC 
TGGAGGAACA AGGTAAAATA CATCAAGCAG ACAACCGCCA TCCTGCAGCA GCGCTACGAA 
GGGGACATCC CTGCTTCCGT GGCTGAGCTG GTAGCCTTGC CAGGTGTTGG GCCCAAGATG 
GCACACTTGG CTATGGCTGT GGCCTGGGGG ACCATATCAG GCATAGCAGT GGACACACAT 
GTGCACAGAA TAGCCAACCG ACTGAGGTGG ACCAAGAAGA TGACCAAGAC CCCAGAAGAG 
ACACGTAAGA ACTTGGAAGA GTGGCTACCC AGGGTGCTGT GGAGTGAGGT CAACGGACTA 
CTGGTAGGCT TCGGCCAACA GATCTGTCTT CCTGTCCATC CTCGATGTCA GGCTTGCCTC 
AACAAGGCCC TGTGCCCTGC TGCCCAGGAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523882.1
CDS	1338..1973
Translation	MRSKKDAPVDQLGAEHCYDASASPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVESILQTDDDTLGRLIYPVGFWRNKVKYIKQTTAILQQRYEGDIPASVAELVALPGVGPKMAHLAMAVAWGTISGIAVDTHVHRIANRLRWTKKMTKTPEETRKNLEEWLPRVLWSEVNGLLVGFGQQICLPVHPRCQACLNKALCPAAQDL

Target ORF information:

RefSeq Version	XM_006523819.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523819.2

ORF Insert Sequence:

ATGAGAAGCA AGAAGGATGC ACCTGTGGAC CAGCTAGGCG CCGAGCACTG CTATGATGCA 
AGTGCCTCCC CGAAGGTGAG GAGGTACCAG GTACTCCTGT CGCTGATGCT CTCCAGCCAG 
ACCAAAGACC AGGTCACAGC AGGTGCTATG CAACGGCTCC GGGCCCGGGG CTTGACTGTG 
GAGAGCATCC TGCAGACCGA TGATGACACG CTAGGCAGAC TCATCTACCC TGTGGGCTTC 
TGGAGGAACA AGGTAAAATA CATCAAGCAG ACAACCGCCA TCCTGCAGCA GCGCTACGAA 
GGGGACATCC CTGCTTCCGT GGCTGAGCTG GTAGCCTTGC CAGGTGTTGG GCCCAAGATG 
GCACACTTGG CTATGGCTGT GGCCTGGGGG ACCATATCAG GCATAGCAGT GGACACACAT 
GTGCACAGAA TAGCCAACCG ACTGAGGTGG ACCAAGAAGA TGACCAAGAC CCCAGAAGAG 
ACACGTAAGA ACTTGGAAGA GTGGCTACCC AGGGTGCTGT GGAGTGAGGT CAACGGACTA 
CTGGTAGGCT TCGGCCAACA GATCTGTCTT CCTGTCCATC CTCGATGTCA GGCTTGCCTC 
AACAAGGCCC TGTGCCCTGC TGCCCAGGAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nthl1 ( XM_006523819.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523882.1 Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53269
Clone ID Related Accession (Same CDS sequence)	XM_006523819.3 , NM_001357615.1 , XM_006523819.2
Accession Version	XM_006523819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	endonuclease III-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006523819.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGCA AGAAGGATGC ACCTGTGGAC CAGCTAGGCG CCGAGCACTG CTATGATGCA 
AGTGCCTCCC CGAAGGTGAG GAGGTACCAG GTACTCCTGT CGCTGATGCT CTCCAGCCAG 
ACCAAAGACC AGGTCACAGC AGGTGCTATG CAACGGCTCC GGGCCCGGGG CTTGACTGTG 
GAGAGCATCC TGCAGACCGA TGATGACACG CTAGGCAGAC TCATCTACCC TGTGGGCTTC 
TGGAGGAACA AGGTAAAATA CATCAAGCAG ACAACCGCCA TCCTGCAGCA GCGCTACGAA 
GGGGACATCC CTGCTTCCGT GGCTGAGCTG GTAGCCTTGC CAGGTGTTGG GCCCAAGATG 
GCACACTTGG CTATGGCTGT GGCCTGGGGG ACCATATCAG GCATAGCAGT GGACACACAT 
GTGCACAGAA TAGCCAACCG ACTGAGGTGG ACCAAGAAGA TGACCAAGAC CCCAGAAGAG 
ACACGTAAGA ACTTGGAAGA GTGGCTACCC AGGGTGCTGT GGAGTGAGGT CAACGGACTA 
CTGGTAGGCT TCGGCCAACA GATCTGTCTT CCTGTCCATC CTCGATGTCA GGCTTGCCTC 
AACAAGGCCC TGTGCCCTGC TGCCCAGGAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523882.1
CDS	1344..1979
Translation	MRSKKDAPVDQLGAEHCYDASASPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVESILQTDDDTLGRLIYPVGFWRNKVKYIKQTTAILQQRYEGDIPASVAELVALPGVGPKMAHLAMAVAWGTISGIAVDTHVHRIANRLRWTKKMTKTPEETRKNLEEWLPRVLWSEVNGLLVGFGQQICLPVHPRCQACLNKALCPAAQDL

Target ORF information:

RefSeq Version	XM_006523819.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus nth (endonuclease III)-like 1 (E.coli) (Nthl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523819.3

ORF Insert Sequence:

ATGAGAAGCA AGAAGGATGC ACCTGTGGAC CAGCTAGGCG CCGAGCACTG CTATGATGCA 
AGTGCCTCCC CGAAGGTGAG GAGGTACCAG GTACTCCTGT CGCTGATGCT CTCCAGCCAG 
ACCAAAGACC AGGTCACAGC AGGTGCTATG CAACGGCTCC GGGCCCGGGG CTTGACTGTG 
GAGAGCATCC TGCAGACCGA TGATGACACG CTAGGCAGAC TCATCTACCC TGTGGGCTTC 
TGGAGGAACA AGGTAAAATA CATCAAGCAG ACAACCGCCA TCCTGCAGCA GCGCTACGAA 
GGGGACATCC CTGCTTCCGT GGCTGAGCTG GTAGCCTTGC CAGGTGTTGG GCCCAAGATG 
GCACACTTGG CTATGGCTGT GGCCTGGGGG ACCATATCAG GCATAGCAGT GGACACACAT 
GTGCACAGAA TAGCCAACCG ACTGAGGTGG ACCAAGAAGA TGACCAAGAC CCCAGAAGAG 
ACACGTAAGA ACTTGGAAGA GTGGCTACCC AGGGTGCTGT GGAGTGAGGT CAACGGACTA 
CTGGTAGGCT TCGGCCAACA GATCTGTCTT CCTGTCCATC CTCGATGTCA GGCTTGCCTC 
AACAAGGCCC TGTGCCCTGC TGCCCAGGAT CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defective repair of oxidative base lesions by the DNA glycosylase Nth1 associates with multiple telomere defects.
PLoS genetics9(7)e1003639(2013)
Vallabhaneni H,O'Callaghan N,Sidorova J,Liu Y

The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells.
BioMed research international2013152909(2013)
Cooley N,Elder RH,Povey AC

The DNA glycosylases Ogg1 and Nth1 do not contribute to Ig class switching in activated mouse splenic B cells.
PloS one7(4)e36061(2012)
Ucher AJ,Linehan EK,Teebor GW,Schrader CE,Stavnezer J

Targeted deletion of the genes encoding NTH1 and NEIL1 DNA N-glycosylases reveals the existence of novel carcinogenic oxidative damage to DNA.
DNA repair8(7)786-94(2009 Jul)
Chan MK,Ocampo-Hafalla MT,Vartanian V,Jaruga P,Kirkali G,Koenig KL,Brown S,Lloyd RS,Dizdaroglu M,Teebor GW

OGG1 initiates age-dependent CAG trinucleotide expansion in somatic cells.
Nature447(7143)447-52(2007 May)
Kovtun IV,Liu Y,Bjoras M,Klungland A,Wilson SH,McMurray CT