P3h2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	P3h2
Entrez Gene ID	210530
Full Name	prolyl 3-hydroxylase 2
Synonyms	4832416N06,AW553532,Leprel1,Mlat4
General protein information	Preferred Names prolyl 3-hydroxylase 2 Names prolyl 3-hydroxylase 2 leprecan-like 1 leprecan-like protein 1 myxoid liposarcoma associated protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_173379.2	NP_775555.1	prolyl 3-hydroxylase 2 precursor
NM_173379.3	NP_775555.1	prolyl 3-hydroxylase 2 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC566697	XP_003201113.1
Xenopus tropicalis (tropical clawed frog)	leprel1	XP_002934088.1
Homo sapiens (human)	LEPREL1	NP_060662.2
Canis lupus familiaris (dog)	LEPREL1	XP_535843.2
Mus musculus (house mouse)	Leprel1	NP_775555.1
Pan troglodytes (chimpanzee)	LEPREL1	XP_001160886.1
Bos taurus (cattle)	LEPREL1	NP_001093815.1
Rattus norvegicus (Norway rat)	Leprel1	NP_001020798.1
Gallus gallus (chicken)	LEPREL1	NP_001001530.1
	LEPREL1	XP_001092797.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Gurdziel K, Vogt KR, Walton KD, Schneider GK, Gumucio DL
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)

Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations.
Hudson DM, Joeng KS, Werther R, Rajagopal A, Weis M, Lee BH, Eyre DR
The Journal of biological chemistry290(13)8613-22(2015 Mar)

Biological role of prolyl 3-hydroxylation in type IV collagen.
Pokidysheva E, Boudko S, Vranka J, Zientek K, Maddox K, Moser M, F?ssler R, Ware J, B?chinger HP
Proceedings of the National Academy of Sciences of the United States of America111(1)161-6(2014 Jan)

Collagen XIV is important for growth and structural integrity of the myocardium.
Tao G, Levay AK, Peacock JD, Huk DJ, Both SN, Purcell NH, Pinto JR, Galantowicz ML, Koch M, Lucchesi PA, Birk DE, Lincoln J
Journal of molecular and cellular cardiology53(5)626-38(2012 Nov)

A role for prolyl 3-hydroxylase 2 in post-translational modification of fibril-forming collagens.
Fernandes RJ, Farnand AW, Traeger GR, Weis MA, Eyre DR
The Journal of biological chemistry286(35)30662-9(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Altered collagen prolyl 3-hydroxylation is caused by loss of P3H2.
Title: Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations.

prolyl 3-hydroxylation of type IV collagen by P3H2 has an important function preventing maternal platelet aggregation in response to the early developing embryo
Title: Biological role of prolyl 3-hydroxylation in type IV collagen.

This report characterizes expression of the P3H1, P3H2 and P3H3 genes in embryonic and adult mice.
Title: Expression of prolyl 3-hydroxylase genes in embryonic and adult mouse tissues.

Study found that all 3 mouse Leprecan genes Lepre1, leprel1 and leprel2 are expressed in the notochord; additionally, they are expressed in dorsal root ganglia, limb buds, branchial arches, and developing kidneys.
Title: Conservation of notochord gene expression across chordates: insights from the Leprecan gene family.

P3H2 is responsible for the hydroxylation of collagen IV, which has the highest 3-hydroxyproline content of all collagens. It is thus possible that P3H2 mutations may lead to a disease with changes in basement membranes.
Title: Characterization of recombinant human prolyl 3-hydroxylase isoenzyme 2, an enzyme modifying the basement membrane collagen IV.

The following P3h2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P3h2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14188	NM_173379.3 Latest version!	Mus musculus prolyl 3-hydroxylase 2 (P3h2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu14188	NM_173379.2	Mus musculus prolyl 3-hydroxylase 2 (P3h2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P3h2 ( NM_173379.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775555.1 Mus musculus prolyl 3-hydroxylase 2 (P3h2), mRNA.

Price & Availability↑

CloneID	OMu14188
Clone ID Related Accession (Same CDS sequence)	NM_173379.2 , NM_173379.3
Accession Version	NM_173379.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC154344.1 and AC109213.6. On Nov 17, 2006 this sequence version replaced NM_173379.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AJ430350.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCGGGAGA GCACGTGGGT GTCGCTGCTG TTGCTGCTAC TGCTGCCCAC ACCGCAGCGG 
GGCGGCCCCC AGGACGGTCG CCGGTCCCCG GAGCCAGAGC CCGAGCGCGG CCCGCTGCAG 
CCCTTCGACC TGCTCTACGC CAGCGGCGTG GCTGCCTACT ACAGCGGCGA CTATGAGCGA 
GCGGTGCGGG ACCTGGAAGC CGCCCTGAGC AGTCACCGGC GCCTACGGGA CATCCGCACG 
CGCTGCGCCC GCCACTGCGC GGCGCGCCGT CCGCTCGCGC CCCCCGGCGC CGGCCCCGGA 
GCCGAGCTGC CCTTCTTCCG CGCGGTGCTG GAGCGGGCGC GCTGCTCCCG CAGCTGCCAG 
AGCCAGCGCC TGGGCGGCCC CGCGTCCCGC CACCGCGTCA GCGAGGACGT GCGCAGCGAC 
TTCCAACGCA GAGTTCCCTA CAACTACCTG CAGAGGGCCT ACATCAAGCT CAACCAGCTG 
GAAAAGGCCA TGGAAGCAGC TCACACGTTC TTCATGGCCA ACCCCGAGCA CATGGAAATG 
CAGCAGGACC TAGAGGACTA TAAAGCCACA GCACGAGTGG AAGCCCCCCT GCTAGACCGC 
GAAGCCAAGC CACACCTGGA AAGCTATAAT GCTGGAGTCA AACATTATGA AGCTGATGAC 
TTTGAATCCG CAATCAAGTA CTTTGAACAA GCTTTAAGAG AGTATTTCAA TGAAGACATG 
GAGTGCCGTG CTCTATGTGA GGGGCCTCAG AGATTTGAGG AATATGAGTA CTTAGGGTAT 
AAAGGTGGTC TTTACGAGGC AATCGCAGAT CACTATATGC AGGTTCTGGT CTGTCAGCAT 
GAATGTGTGA GGGAACTCGC CACCCGCCCA GGCCGCCTCT CACCAATCGA GAACTTTCTT 
CCCTTGCATT ACGACTACCT CCAGTTTGCC TACTACAGAG TTGGTGAATA TGTGAAAGCC 
TTGGAGTGCG CCAAAGCCTA CCTGATGTTC CATCCAGACA ATGAGGATGT CCTGGACAAT 
GTAGACTTCT ATGAGAGTCT GCTGGATGAC AGCACTGATC CAGCATCCAT TGAGGCCCGG 
GAGGATTTAA CAGCATTTGT CAAACGTCAT AAACTTGAAG CTGAACTGAT CAAGTTAGCT 
GCAGAGGGCC TGGGATTTTC ATATGCTGAA CCGAATTACT GGATCAGCTA TGGAGGACGG 
CAGGATGAGA ACCGAGTCCC TTCAGGAGTG AATATGGATG GGGCAGAAGT TCATGGATTG 
TCAATGGGAA AGAAGTCACC GCCCAAGATA GGTCGAGACC TAAGAGAAGG TGGCCCTCTG 
CTCTACGAGA ACATCACCTT CGTCTACAAC TCTGAGCAGC TGAACGGGAC TCAACGGGTT 
CTTCTCGATA ATGTCCTGTC GCAAGAGCAG TGTCGGGAGC TGCACAGTGT GGCCAATGGA 
ATCATGCTGG TTGGTGATGG ATACCGAGGG AAAACGTCAC CCCACACACC CAATGAGAAG 
TTTGAAGGAG CAACTGTTTT AAAAGCACTC AAATTTGGTT ATGAAGGCCG GGTGCCACTG 
AAGAGTGCAA GACTGTTCTA TGACATCAGC GAGAAGGCTC GGAAGATTGT GGAATCCTAT 
TTCATGCTGA ACTCAACCCT GTATTTTTCC TATACACACA TGGTCTGCAG AACAGCCCTG 
TCTGGGCAGC AGGATCGAAG AAATGACCTC AGTCACCCCA TTCATGCAGA CAACTGCCTG 
CTGGATCCAG AAGCCAATGA ATGCTGGAAG GAGCCTCCCG CTTACACCTT TCGGGACTAC 
AGTGCTCTCC TGTATATGAA TGACGACTTT GACGGAGGGG AATTCATCTT CACAGAAATG 
GATGCTAAAA CCGTGACTGC CTCTATAAAA CCAAAGTGTG GCCGAATGAT CAGCTTCTCG 
TCTGGCGGCG AGAACCCTCA TGGCGTGAAG GCGGTCACCA GAGGCCAGCG CTGTGCTGTG 
GCACTATGGT TTACACTGGA CCCTCTTTAC CGAGAACTGG AGCGGATACA GGCAGATGAA 
GTGATTGCCA TCTTGGATCA AGAACAGCGC GGGAAGCATG GACTGAATAT TAACCCCAAA 
GATGAGCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775555.1
CDS	102..2213
Translation	MRESTWVSLLLLLLLPTPQRGGPQDGRRSPEPEPERGPLQPFDLLYASGVAAYYSGDYERAVRDLEAALSSHRRLRDIRTRCARHCAARRPLAPPGAGPGAELPFFRAVLERARCSRSCQSQRLGGPASRHRVSEDVRSDFQRRVPYNYLQRAYIKLNQLEKAMEAAHTFFMANPEHMEMQQDLEDYKATARVEAPLLDREAKPHLESYNAGVKHYEADDFESAIKYFEQALREYFNEDMECRALCEGPQRFEEYEYLGYKGGLYEAIADHYMQVLVCQHECVRELATRPGRLSPIENFLPLHYDYLQFAYYRVGEYVKALECAKAYLMFHPDNEDVLDNVDFYESLLDDSTDPASIEAREDLTAFVKRHKLEAELIKLAAEGLGFSYAEPNYWISYGGRQDENRVPSGVNMDGAEVHGLSMGKKSPPKIGRDLREGGPLLYENITFVYNSEQLNGTQRVLLDNVLSQEQCRELHSVANGIMLVGDGYRGKTSPHTPNEKFEGATVLKALKFGYEGRVPLKSARLFYDISEKARKIVESYFMLNSTLYFSYTHMVCRTALSGQQDRRNDLSHPIHADNCLLDPEANECWKEPPAYTFRDYSALLYMNDDFDGGEFIFTEMDAKTVTASIKPKCGRMISFSSGGENPHGVKAVTRGQRCAVALWFTLDPLYRELERIQADEVIAILDQEQRGKHGLNINPKDEL

Target ORF information:

RefSeq Version	NM_173379.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 2 (P3h2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173379.2

ORF Insert Sequence:

ATGCGGGAGA GCACGTGGGT GTCGCTGCTG TTGCTGCTAC TGCTGCCCAC ACCGCAGCGG 
GGCGGCCCCC AGGACGGTCG CCGGTCCCCG GAGCCAGAGC CCGAGCGCGG CCCGCTGCAG 
CCCTTCGACC TGCTCTACGC CAGCGGCGTG GCTGCCTACT ACAGCGGCGA CTATGAGCGA 
GCGGTGCGGG ACCTGGAAGC CGCCCTGAGC AGTCACCGGC GCCTACGGGA CATCCGCACG 
CGCTGCGCCC GCCACTGCGC GGCGCGCCGT CCGCTCGCGC CCCCCGGCGC CGGCCCCGGA 
GCCGAGCTGC CCTTCTTCCG CGCGGTGCTG GAGCGGGCGC GCTGCTCCCG CAGCTGCCAG 
AGCCAGCGCC TGGGCGGCCC CGCGTCCCGC CACCGCGTCA GCGAGGACGT GCGCAGCGAC 
TTCCAACGCA GAGTTCCCTA CAACTACCTG CAGAGGGCCT ACATCAAGCT CAACCAGCTG 
GAAAAGGCCA TGGAAGCAGC TCACACGTTC TTCATGGCCA ACCCCGAGCA CATGGAAATG 
CAGCAGGACC TAGAGGACTA TAAAGCCACA GCACGAGTGG AAGCCCCCCT GCTAGACCGC 
GAAGCCAAGC CACACCTGGA AAGCTATAAT GCTGGAGTCA AACATTATGA AGCTGATGAC 
TTTGAATCCG CAATCAAGTA CTTTGAACAA GCTTTAAGAG AGTATTTCAA TGAAGACATG 
GAGTGCCGTG CTCTATGTGA GGGGCCTCAG AGATTTGAGG AATATGAGTA CTTAGGGTAT 
AAAGGTGGTC TTTACGAGGC AATCGCAGAT CACTATATGC AGGTTCTGGT CTGTCAGCAT 
GAATGTGTGA GGGAACTCGC CACCCGCCCA GGCCGCCTCT CACCAATCGA GAACTTTCTT 
CCCTTGCATT ACGACTACCT CCAGTTTGCC TACTACAGAG TTGGTGAATA TGTGAAAGCC 
TTGGAGTGCG CCAAAGCCTA CCTGATGTTC CATCCAGACA ATGAGGATGT CCTGGACAAT 
GTAGACTTCT ATGAGAGTCT GCTGGATGAC AGCACTGATC CAGCATCCAT TGAGGCCCGG 
GAGGATTTAA CAGCATTTGT CAAACGTCAT AAACTTGAAG CTGAACTGAT CAAGTTAGCT 
GCAGAGGGCC TGGGATTTTC ATATGCTGAA CCGAATTACT GGATCAGCTA TGGAGGACGG 
CAGGATGAGA ACCGAGTCCC TTCAGGAGTG AATATGGATG GGGCAGAAGT TCATGGATTG 
TCAATGGGAA AGAAGTCACC GCCCAAGATA GGTCGAGACC TAAGAGAAGG TGGCCCTCTG 
CTCTACGAGA ACATCACCTT CGTCTACAAC TCTGAGCAGC TGAACGGGAC TCAACGGGTT 
CTTCTCGATA ATGTCCTGTC GCAAGAGCAG TGTCGGGAGC TGCACAGTGT GGCCAATGGA 
ATCATGCTGG TTGGTGATGG ATACCGAGGG AAAACGTCAC CCCACACACC CAATGAGAAG 
TTTGAAGGAG CAACTGTTTT AAAAGCACTC AAATTTGGTT ATGAAGGCCG GGTGCCACTG 
AAGAGTGCAA GACTGTTCTA TGACATCAGC GAGAAGGCTC GGAAGATTGT GGAATCCTAT 
TTCATGCTGA ACTCAACCCT GTATTTTTCC TATACACACA TGGTCTGCAG AACAGCCCTG 
TCTGGGCAGC AGGATCGAAG AAATGACCTC AGTCACCCCA TTCATGCAGA CAACTGCCTG 
CTGGATCCAG AAGCCAATGA ATGCTGGAAG GAGCCTCCCG CTTACACCTT TCGGGACTAC 
AGTGCTCTCC TGTATATGAA TGACGACTTT GACGGAGGGG AATTCATCTT CACAGAAATG 
GATGCTAAAA CCGTGACTGC CTCTATAAAA CCAAAGTGTG GCCGAATGAT CAGCTTCTCG 
TCTGGCGGCG AGAACCCTCA TGGCGTGAAG GCGGTCACCA GAGGCCAGCG CTGTGCTGTG 
GCACTATGGT TTACACTGGA CCCTCTTTAC CGAGAACTGG AGCGGATACA GGCAGATGAA 
GTGATTGCCA TCTTGGATCA AGAACAGCGC GGGAAGCATG GACTGAATAT TAACCCCAAA 
GATGAGCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P3h2 ( NM_173379.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775555.1 Mus musculus prolyl 3-hydroxylase 2 (P3h2), mRNA.

Price & Availability↑

CloneID	OMu14188
Clone ID Related Accession (Same CDS sequence)	NM_173379.2 , NM_173379.3
Accession Version	NM_173379.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	prolyl 3-hydroxylase 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154344.1 and AC109213.6. On Apr 20, 2018 this sequence version replaced NM_173379.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AJ430350.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGAGA GCACGTGGGT GTCGCTGCTG TTGCTGCTAC TGCTGCCCAC ACCGCAGCGG 
GGCGGCCCCC AGGACGGTCG CCGGTCCCCG GAGCCAGAGC CCGAGCGCGG CCCGCTGCAG 
CCCTTCGACC TGCTCTACGC CAGCGGCGTG GCTGCCTACT ACAGCGGCGA CTATGAGCGA 
GCGGTGCGGG ACCTGGAAGC CGCCCTGAGC AGTCACCGGC GCCTACGGGA CATCCGCACG 
CGCTGCGCCC GCCACTGCGC GGCGCGCCGT CCGCTCGCGC CCCCCGGCGC CGGCCCCGGA 
GCCGAGCTGC CCTTCTTCCG CGCGGTGCTG GAGCGGGCGC GCTGCTCCCG CAGCTGCCAG 
AGCCAGCGCC TGGGCGGCCC CGCGTCCCGC CACCGCGTCA GCGAGGACGT GCGCAGCGAC 
TTCCAACGCA GAGTTCCCTA CAACTACCTG CAGAGGGCCT ACATCAAGCT CAACCAGCTG 
GAAAAGGCCA TGGAAGCAGC TCACACGTTC TTCATGGCCA ACCCCGAGCA CATGGAAATG 
CAGCAGGACC TAGAGGACTA TAAAGCCACA GCACGAGTGG AAGCCCCCCT GCTAGACCGC 
GAAGCCAAGC CACACCTGGA AAGCTATAAT GCTGGAGTCA AACATTATGA AGCTGATGAC 
TTTGAATCCG CAATCAAGTA CTTTGAACAA GCTTTAAGAG AGTATTTCAA TGAAGACATG 
GAGTGCCGTG CTCTATGTGA GGGGCCTCAG AGATTTGAGG AATATGAGTA CTTAGGGTAT 
AAAGGTGGTC TTTACGAGGC AATCGCAGAT CACTATATGC AGGTTCTGGT CTGTCAGCAT 
GAATGTGTGA GGGAACTCGC CACCCGCCCA GGCCGCCTCT CACCAATCGA GAACTTTCTT 
CCCTTGCATT ACGACTACCT CCAGTTTGCC TACTACAGAG TTGGTGAATA TGTGAAAGCC 
TTGGAGTGCG CCAAAGCCTA CCTGATGTTC CATCCAGACA ATGAGGATGT CCTGGACAAT 
GTAGACTTCT ATGAGAGTCT GCTGGATGAC AGCACTGATC CAGCATCCAT TGAGGCCCGG 
GAGGATTTAA CAGCATTTGT CAAACGTCAT AAACTTGAAG CTGAACTGAT CAAGTTAGCT 
GCAGAGGGCC TGGGATTTTC ATATGCTGAA CCGAATTACT GGATCAGCTA TGGAGGACGG 
CAGGATGAGA ACCGAGTCCC TTCAGGAGTG AATATGGATG GGGCAGAAGT TCATGGATTG 
TCAATGGGAA AGAAGTCACC GCCCAAGATA GGTCGAGACC TAAGAGAAGG TGGCCCTCTG 
CTCTACGAGA ACATCACCTT CGTCTACAAC TCTGAGCAGC TGAACGGGAC TCAACGGGTT 
CTTCTCGATA ATGTCCTGTC GCAAGAGCAG TGTCGGGAGC TGCACAGTGT GGCCAATGGA 
ATCATGCTGG TTGGTGATGG ATACCGAGGG AAAACGTCAC CCCACACACC CAATGAGAAG 
TTTGAAGGAG CAACTGTTTT AAAAGCACTC AAATTTGGTT ATGAAGGCCG GGTGCCACTG 
AAGAGTGCAA GACTGTTCTA TGACATCAGC GAGAAGGCTC GGAAGATTGT GGAATCCTAT 
TTCATGCTGA ACTCAACCCT GTATTTTTCC TATACACACA TGGTCTGCAG AACAGCCCTG 
TCTGGGCAGC AGGATCGAAG AAATGACCTC AGTCACCCCA TTCATGCAGA CAACTGCCTG 
CTGGATCCAG AAGCCAATGA ATGCTGGAAG GAGCCTCCCG CTTACACCTT TCGGGACTAC 
AGTGCTCTCC TGTATATGAA TGACGACTTT GACGGAGGGG AATTCATCTT CACAGAAATG 
GATGCTAAAA CCGTGACTGC CTCTATAAAA CCAAAGTGTG GCCGAATGAT CAGCTTCTCG 
TCTGGCGGCG AGAACCCTCA TGGCGTGAAG GCGGTCACCA GAGGCCAGCG CTGTGCTGTG 
GCACTATGGT TTACACTGGA CCCTCTTTAC CGAGAACTGG AGCGGATACA GGCAGATGAA 
GTGATTGCCA TCTTGGATCA AGAACAGCGC GGGAAGCATG GACTGAATAT TAACCCCAAA 
GATGAGCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775555.1
CDS	131..2242
Translation	MRESTWVSLLLLLLLPTPQRGGPQDGRRSPEPEPERGPLQPFDLLYASGVAAYYSGDYERAVRDLEAALSSHRRLRDIRTRCARHCAARRPLAPPGAGPGAELPFFRAVLERARCSRSCQSQRLGGPASRHRVSEDVRSDFQRRVPYNYLQRAYIKLNQLEKAMEAAHTFFMANPEHMEMQQDLEDYKATARVEAPLLDREAKPHLESYNAGVKHYEADDFESAIKYFEQALREYFNEDMECRALCEGPQRFEEYEYLGYKGGLYEAIADHYMQVLVCQHECVRELATRPGRLSPIENFLPLHYDYLQFAYYRVGEYVKALECAKAYLMFHPDNEDVLDNVDFYESLLDDSTDPASIEAREDLTAFVKRHKLEAELIKLAAEGLGFSYAEPNYWISYGGRQDENRVPSGVNMDGAEVHGLSMGKKSPPKIGRDLREGGPLLYENITFVYNSEQLNGTQRVLLDNVLSQEQCRELHSVANGIMLVGDGYRGKTSPHTPNEKFEGATVLKALKFGYEGRVPLKSARLFYDISEKARKIVESYFMLNSTLYFSYTHMVCRTALSGQQDRRNDLSHPIHADNCLLDPEANECWKEPPAYTFRDYSALLYMNDDFDGGEFIFTEMDAKTVTASIKPKCGRMISFSSGGENPHGVKAVTRGQRCAVALWFTLDPLYRELERIQADEVIAILDQEQRGKHGLNINPKDEL

Target ORF information:

RefSeq Version	NM_173379.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus prolyl 3-hydroxylase 2 (P3h2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173379.3

ORF Insert Sequence:

ATGCGGGAGA GCACGTGGGT GTCGCTGCTG TTGCTGCTAC TGCTGCCCAC ACCGCAGCGG 
GGCGGCCCCC AGGACGGTCG CCGGTCCCCG GAGCCAGAGC CCGAGCGCGG CCCGCTGCAG 
CCCTTCGACC TGCTCTACGC CAGCGGCGTG GCTGCCTACT ACAGCGGCGA CTATGAGCGA 
GCGGTGCGGG ACCTGGAAGC CGCCCTGAGC AGTCACCGGC GCCTACGGGA CATCCGCACG 
CGCTGCGCCC GCCACTGCGC GGCGCGCCGT CCGCTCGCGC CCCCCGGCGC CGGCCCCGGA 
GCCGAGCTGC CCTTCTTCCG CGCGGTGCTG GAGCGGGCGC GCTGCTCCCG CAGCTGCCAG 
AGCCAGCGCC TGGGCGGCCC CGCGTCCCGC CACCGCGTCA GCGAGGACGT GCGCAGCGAC 
TTCCAACGCA GAGTTCCCTA CAACTACCTG CAGAGGGCCT ACATCAAGCT CAACCAGCTG 
GAAAAGGCCA TGGAAGCAGC TCACACGTTC TTCATGGCCA ACCCCGAGCA CATGGAAATG 
CAGCAGGACC TAGAGGACTA TAAAGCCACA GCACGAGTGG AAGCCCCCCT GCTAGACCGC 
GAAGCCAAGC CACACCTGGA AAGCTATAAT GCTGGAGTCA AACATTATGA AGCTGATGAC 
TTTGAATCCG CAATCAAGTA CTTTGAACAA GCTTTAAGAG AGTATTTCAA TGAAGACATG 
GAGTGCCGTG CTCTATGTGA GGGGCCTCAG AGATTTGAGG AATATGAGTA CTTAGGGTAT 
AAAGGTGGTC TTTACGAGGC AATCGCAGAT CACTATATGC AGGTTCTGGT CTGTCAGCAT 
GAATGTGTGA GGGAACTCGC CACCCGCCCA GGCCGCCTCT CACCAATCGA GAACTTTCTT 
CCCTTGCATT ACGACTACCT CCAGTTTGCC TACTACAGAG TTGGTGAATA TGTGAAAGCC 
TTGGAGTGCG CCAAAGCCTA CCTGATGTTC CATCCAGACA ATGAGGATGT CCTGGACAAT 
GTAGACTTCT ATGAGAGTCT GCTGGATGAC AGCACTGATC CAGCATCCAT TGAGGCCCGG 
GAGGATTTAA CAGCATTTGT CAAACGTCAT AAACTTGAAG CTGAACTGAT CAAGTTAGCT 
GCAGAGGGCC TGGGATTTTC ATATGCTGAA CCGAATTACT GGATCAGCTA TGGAGGACGG 
CAGGATGAGA ACCGAGTCCC TTCAGGAGTG AATATGGATG GGGCAGAAGT TCATGGATTG 
TCAATGGGAA AGAAGTCACC GCCCAAGATA GGTCGAGACC TAAGAGAAGG TGGCCCTCTG 
CTCTACGAGA ACATCACCTT CGTCTACAAC TCTGAGCAGC TGAACGGGAC TCAACGGGTT 
CTTCTCGATA ATGTCCTGTC GCAAGAGCAG TGTCGGGAGC TGCACAGTGT GGCCAATGGA 
ATCATGCTGG TTGGTGATGG ATACCGAGGG AAAACGTCAC CCCACACACC CAATGAGAAG 
TTTGAAGGAG CAACTGTTTT AAAAGCACTC AAATTTGGTT ATGAAGGCCG GGTGCCACTG 
AAGAGTGCAA GACTGTTCTA TGACATCAGC GAGAAGGCTC GGAAGATTGT GGAATCCTAT 
TTCATGCTGA ACTCAACCCT GTATTTTTCC TATACACACA TGGTCTGCAG AACAGCCCTG 
TCTGGGCAGC AGGATCGAAG AAATGACCTC AGTCACCCCA TTCATGCAGA CAACTGCCTG 
CTGGATCCAG AAGCCAATGA ATGCTGGAAG GAGCCTCCCG CTTACACCTT TCGGGACTAC 
AGTGCTCTCC TGTATATGAA TGACGACTTT GACGGAGGGG AATTCATCTT CACAGAAATG 
GATGCTAAAA CCGTGACTGC CTCTATAAAA CCAAAGTGTG GCCGAATGAT CAGCTTCTCG 
TCTGGCGGCG AGAACCCTCA TGGCGTGAAG GCGGTCACCA GAGGCCAGCG CTGTGCTGTG 
GCACTATGGT TTACACTGGA CCCTCTTTAC CGAGAACTGG AGCGGATACA GGCAGATGAA 
GTGATTGCCA TCTTGGATCA AGAACAGCGC GGGAAGCATG GACTGAATAT TAACCCCAAA 
GATGAGCTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome of the inner circular smooth muscle of the developing mouse intestine: Evidence for regulation of visceral smooth muscle genes by the hedgehog target gene, cJun.
Developmental dynamics : an official publication of the American Association of Anatomists245(5)614-26(2016 May)
Gurdziel K,Vogt KR,Walton KD,Schneider GK,Gumucio DL

Post-translationally abnormal collagens of prolyl 3-hydroxylase-2 null mice offer a pathobiological mechanism for the high myopia linked to human LEPREL1 mutations.
The Journal of biological chemistry290(13)8613-22(2015 Mar)
Hudson DM,Joeng KS,Werther R,Rajagopal A,Weis M,Lee BH,Eyre DR

Biological role of prolyl 3-hydroxylation in type IV collagen.
Proceedings of the National Academy of Sciences of the United States of America111(1)161-6(2014 Jan)
Pokidysheva E,Boudko S,Vranka J,Zientek K,Maddox K,Moser M,F?ssler R,Ware J,B?chinger HP

Collagen XIV is important for growth and structural integrity of the myocardium.
Journal of molecular and cellular cardiology53(5)626-38(2012 Nov)
Tao G,Levay AK,Peacock JD,Huk DJ,Both SN,Purcell NH,Pinto JR,Galantowicz ML,Koch M,Lucchesi PA,Birk DE,Lincoln J

A role for prolyl 3-hydroxylase 2 in post-translational modification of fibril-forming collagens.
The Journal of biological chemistry286(35)30662-9(2011 Sep)
Fernandes RJ,Farnand AW,Traeger GR,Weis MA,Eyre DR

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P3h2 cDNA ORF clone, Mus musculus(house mouse)

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