Prss27 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prss27
Entrez Gene ID	213171
Full Name	protease, serine 27
Synonyms	CAPH2,Mpn,marapsin
General protein information	Preferred Names protease, serine 27 Names serine protease 27 channel-activating protease 2-like protein pancreasin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A3.3\|17 12.21 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_175440.4	NP_780649.1	serine protease 27 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prss27	NP_891994.3
Danio rerio (zebrafish)	LOC100001520	XP_001341498.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004569	XP_001237553.3
Homo sapiens (human)	PRSS27	NP_114154.1
	PRSS27	XP_001086389.1
Canis lupus familiaris (dog)	PRSS27	XP_005621733.1
Bos taurus (cattle)	PRSS27	XP_005224679.1
Danio rerio (zebrafish)	zgc:92313	NP_001002596.1
Xenopus tropicalis (tropical clawed frog)	LOC100495333	XP_002940815.1
Xenopus tropicalis (tropical clawed frog)	LOC101734975	XP_004918402.1
Pan troglodytes (chimpanzee)	PRSS27	XP_510751.2
Mus musculus (house mouse)	Prss27	NP_780649.1

Gene Ontology
Bibliography

Related articles in PubMed

Tbx18 Regulates the Differentiation of Periductal Smooth Muscle Stroma and the Maintenance of Epithelial Integrity in the Prostate.
Bolt CC, Negi S, Guimar?es-Camboa N, Zhang H, Troy JM, Lu X, Kispert A, Evans SM, Stubbs L
PloS one11(4)e0154413(2016)

Mutational tail loss is an evolutionary mechanism for liberating marapsins and other type I serine proteases from transmembrane anchors.
Raman K, Trivedi NN, Raymond WW, Ganesan R, Kirchhofer D, Verghese GM, Craik CS, Schneider EL, Nimishakavi S, Caughey GH
The Journal of biological chemistry288(15)10588-98(2013 Apr)

The serine protease marapsin is expressed in stratified squamous epithelia and is up-regulated in the hyperproliferative epidermis of psoriasis and regenerating wounds.
Li W, Danilenko DM, Bunting S, Ganesan R, Sa S, Ferrando R, Wu TD, Kolumam GA, Ouyang W, Kirchhofer D
The Journal of biological chemistry284(1)218-28(2009 Jan)

Identification of a subgroup of glycosylphosphatidylinositol-anchored tryptases.
Wong GW, Stevens RL
Biochemical and biophysical research communications336(2)579-84(2005 Oct)

Mouse chromosome 17A3.3 contains 13 genes that encode functional tryptic-like serine proteases with distinct tissue and cell expression patterns.
Wong GW, Yasuda S, Morokawa N, Li L, Stevens RL
The Journal of biological chemistry279(4)2438-52(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutational tail loss is an evolutionary mechanism for liberating marapsins and other type I serine proteases from transmembrane anchors.
Title: Mutational tail loss is an evolutionary mechanism for liberating marapsins and other type I serine proteases from transmembrane anchors.

Marapsin's restricted expression, localization, and cytokine-inducible expression suggest a role in the terminal differentiation of keratinocytes in hyperproliferating squamous epithelia.
Title: The serine protease marapsin is expressed in stratified squamous epithelia and is up-regulated in the hyperproliferative epidermis of psoriasis and regenerating wounds.

The following Prss27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prss27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18602	NM_175440.4 Latest version!	Mus musculus protease, serine 27 (Prss27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prss27 ( NM_175440.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780649.1 Mus musculus protease, serine 27 (Prss27), mRNA.

Price & Availability↑

CloneID	OMu18602
Clone ID Related Accession (Same CDS sequence)	NM_175440.4
Accession Version	NM_175440.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	serine protease 27 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT010502.14. On May 2, 2007 this sequence version replaced NM_175440.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK080281.1, BC117009.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCAGC CCCACATCGC TGCCCTGCTG CTGCTGCCGC TGCTGCTCAG ATCTGGGACT 
GAAGGGGCCA GGACTCTGAG AGCCTGTGGG CATCCAAAGA TGTTCAACCG GATGGTGGGC 
GGGGAGAACG CCTTAGAAGG TGAGTGGCCC TGGCAGGTCA GCATCCAGCG CAACGGAATC 
CACTTCTGTG GGGGCAGTCT CATCGCACCA ACATGGGTCC TCACTGCTGC ACACTGCTTC 
TCCAACACTT CGGACATATC CATATACCAG GTCCTGCTGG GGGCACTGAA GCTGCAGCAG 
CCAGGACCAC ACGCCTTGTA TGTCCCCGTG AAGCAGGTGA AAAGCAATCC TCAGTACCAA 
GGCATGGCCT CCAGTGCCGA CGTGGCTCTG GTGGAGCTGC AGGGGCCGGT GACCTTCACC 
AATTACATCC TTCCTGTGTG CCTTCCTGAT CCCTCGGTCA TCTTTGAGTC GGGCATGAAC 
TGCTGGGTCA CTGGCTGGGG CAGCCCCAGT GAACAAGACC GACTACCCAA CCCACGGGTC 
CTGCAGAAAC TTGCTGTGCC CATCATTGAC ACGCCCAAGT GCAACCTGCT GTACAACAAA 
GATGTTGAGT CTGACTTCCA GCTCAAAACC ATCAAGGATG ACATGCTGTG TGCAGGCTTC 
GCAGAGGGCA AGAAGGACGC CTGTAAGGGT GACTCTGGAG GCCCACTGGT GTGCCTTGTG 
GACCAGTCGT GGGTGCAGGC TGGGGTGATC AGCTGGGGAG AGGGTTGTGC CCGTCGGAAT 
CGCCCAGGTG TCTACATCCG TGTGACTTCT CATCACAAAT GGATCCACCA AATCATCCCA 
GAACTGCAGT TTCAGGGGAG AGCAGGCACC CAGCAGCAAC AAAAAGACTC CCAAGGTCAG 
CAGCGCCTGG CGGGGAACTC CGCCCCTTGC CTGGCTGCCC ATGCAATGGT GCTGGCCCTG 
GGAGCACTGC TGCTCAGGAT CGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780649.1
CDS	65..1051
Translation	MRQPHIAALLLLPLLLRSGTEGARTLRACGHPKMFNRMVGGENALEGEWPWQVSIQRNGIHFCGGSLIAPTWVLTAAHCFSNTSDISIYQVLLGALKLQQPGPHALYVPVKQVKSNPQYQGMASSADVALVELQGPVTFTNYILPVCLPDPSVIFESGMNCWVTGWGSPSEQDRLPNPRVLQKLAVPIIDTPKCNLLYNKDVESDFQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVTSHHKWIHQIIPELQFQGRAGTQQQQKDSQGQQRLAGNSAPCLAAHAMVLALGALLLRIV

Target ORF information:

RefSeq Version	NM_175440.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease, serine 27 (Prss27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175440.4

ORF Insert Sequence:

ATGAGGCAGC CCCACATCGC TGCCCTGCTG CTGCTGCCGC TGCTGCTCAG ATCTGGGACT 
GAAGGGGCCA GGACTCTGAG AGCCTGTGGG CATCCAAAGA TGTTCAACCG GATGGTGGGC 
GGGGAGAACG CCTTAGAAGG TGAGTGGCCC TGGCAGGTCA GCATCCAGCG CAACGGAATC 
CACTTCTGTG GGGGCAGTCT CATCGCACCA ACATGGGTCC TCACTGCTGC ACACTGCTTC 
TCCAACACTT CGGACATATC CATATACCAG GTCCTGCTGG GGGCACTGAA GCTGCAGCAG 
CCAGGACCAC ACGCCTTGTA TGTCCCCGTG AAGCAGGTGA AAAGCAATCC TCAGTACCAA 
GGCATGGCCT CCAGTGCCGA CGTGGCTCTG GTGGAGCTGC AGGGGCCGGT GACCTTCACC 
AATTACATCC TTCCTGTGTG CCTTCCTGAT CCCTCGGTCA TCTTTGAGTC GGGCATGAAC 
TGCTGGGTCA CTGGCTGGGG CAGCCCCAGT GAACAAGACC GACTACCCAA CCCACGGGTC 
CTGCAGAAAC TTGCTGTGCC CATCATTGAC ACGCCCAAGT GCAACCTGCT GTACAACAAA 
GATGTTGAGT CTGACTTCCA GCTCAAAACC ATCAAGGATG ACATGCTGTG TGCAGGCTTC 
GCAGAGGGCA AGAAGGACGC CTGTAAGGGT GACTCTGGAG GCCCACTGGT GTGCCTTGTG 
GACCAGTCGT GGGTGCAGGC TGGGGTGATC AGCTGGGGAG AGGGTTGTGC CCGTCGGAAT 
CGCCCAGGTG TCTACATCCG TGTGACTTCT CATCACAAAT GGATCCACCA AATCATCCCA 
GAACTGCAGT TTCAGGGGAG AGCAGGCACC CAGCAGCAAC AAAAAGACTC CCAAGGTCAG 
CAGCGCCTGG CGGGGAACTC CGCCCCTTGC CTGGCTGCCC ATGCAATGGT GCTGGCCCTG 
GGAGCACTGC TGCTCAGGAT CGTCTAG