Timm44 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Timm44
Entrez Gene ID	21856
Full Name	translocase of inner mitochondrial membrane 44
Synonyms	0710005E20Rik,D8Ertd118e,Mimt44,Tim44
General protein information	Preferred Names translocase of inner mitochondrial membrane 44 Names mitochondrial import inner membrane translocase subunit TIM44 translocator of inner mitochondrial membrane 44 translocator of inner mitochondrial membrane 44; DNA segment, Chr 8, ERATO Doi 118, expressed
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 A1.1\|8 1.99 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011592.2	NP_035722.2	mitochondrial import inner membrane translocase subunit TIM44

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TIMM44	XP_418174.2
Homo sapiens (human)	TIMM44	NP_006342.2
Canis lupus familiaris (dog)	TIMM44	XP_542123.2
Rattus norvegicus (Norway rat)	Timm44	NP_058963.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000291	XP_310830.4
Caenorhabditis elegans (roundworm)	T09B4.9	NP_491780.1
Eremothecium gossypii	AGOS_ADL118C	NP_983978.1
Bos taurus (cattle)	TIMM44	NP_001179583.1
Danio rerio (zebrafish)	LOC100537910	XP_003201001.1
Magnaporthe oryzae (rice blast fungus)	MGG_11419	XP_003713412.1
	TIMM44	XP_001098363.2
Danio rerio (zebrafish)	timm44	NP_001013525.1
Kluyveromyces lactis	KLLA0D16214g	XP_453775.1
Schizosaccharomyces pombe (fission yeast)	tim44	NP_595905.1
Xenopus tropicalis (tropical clawed frog)	timm44	NP_001120395.1
Pan troglodytes (chimpanzee)	TIMM44	XP_003316109.1
Mus musculus (house mouse)	Timm44	NP_035722.2
Drosophila melanogaster (fruit fly)	CG11779	NP_001027193.2
Saccharomyces cerevisiae (baker's yeast)	TIM44	NP_012242.1
Neurospora crassa	NCU03229	XP_965329.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a fluorescent small-molecule enhancer for therapeutic autophagy in colorectal cancer by targeting mitochondrial protein translocase TIM44.
Huang Y, Zhou J, Luo S, Wang Y, He J, Luo P, Chen Z, Liu T, Tan X, Ou J, Miao H, Liang H, Shi C
Gut67(2)307-319(2018 Feb)

Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease.
Fakruddin M, Wei FY, Suzuki T, Asano K, Kaieda T, Omori A, Izumi R, Fujimura A, Kaitsuka T, Miyata K, Araki K, Oike Y, Scorrano L, Suzuki T, Tomizawa K
Cell reports22(2)482-496(2018 Jan)

Dynamics of mitochondrial DNA nucleoids regulated by mitochondrial fission is essential for maintenance of homogeneously active mitochondria during neonatal heart development.
Ishihara T, Ban-Ishihara R, Maeda M, Matsunaga Y, Ichimura A, Kyogoku S, Aoki H, Katada S, Nakada K, Nomura M, Mizushima N, Mihara K, Ishihara N
Molecular and cellular biology35(1)211-23(2015 Jan)

Mitochondrial fission factor Drp1 is essential for embryonic development and synapse formation in mice.
Ishihara N, Nomura M, Jofuku A, Kato H, Suzuki SO, Masuda K, Otera H, Nakanishi Y, Nonaka I, Goto Y, Taguchi N, Morinaga H, Maeda M, Takayanagi R, Yokota S, Mihara K
Nature cell biology11(8)958-66(2009 Aug)

Neurological phenotype and reduced lifespan in heterozygous Tim23 knockout mice, the first mouse model of defective mitochondrial import.
Ahting U, Floss T, Uez N, Schneider-Lohmar I, Becker L, Kling E, Iuso A, Bender A, de Angelis MH, Gailus-Durner V, Fuchs H, Meitinger T, Wurst W, Prokisch H, Klopstock T
Biochimica et biophysica acta1787(5)371-6(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Timm44 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Timm44 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu15448	NM_011592.2 Latest version!	Mus musculus translocase of inner mitochondrial membrane 44 (Timm44), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Timm44 ( NM_011592.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035722.2 Mus musculus translocase of inner mitochondrial membrane 44 (Timm44), mRNA.

Price & Availability↑

CloneID	OMu15448
Clone ID Related Accession (Same CDS sequence)	NM_011592.2
Accession Version	NM_011592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	mitochondrial import inner membrane translocase subunit TIM44
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA493459.1, BC117523.1 and BQ031430.1. On Mar 25, 2008 this sequence version replaced NM_011592.1. ##Evidence-Data-START## Transcript exon combination :: BC023454.1, BC110677.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CAGCTCTGCG GGGTGGCTGG TGCCGCTGTC CGCGGAGGTG CCTAGGCAGC 
GGAATCCAAT TTCTCTCCAG CCACAACCTA CCCCATGGTT CAAGCTACCA GATAAGCCGG 
CCAGGAAGAG AGCTGACACT GACCAAATCT TATTCTTCTG GGAGCAGAAA AGGGTTTTTG 
TCAGGCTTGC TAGATAACAT CAAACAAGAA TTAGCCAAAA ACAAAGAGAT GAAAGAAAGT 
ATAAAAAAGT TCCGAGATGA GGCCAAGAAG CTAGAAGAGT CGGATGCTCT CCAGGAAGCC 
AGAAGGAAGT ATAAATCCAT CGAATCAGAA ACTGTGCGTA CCAGTGAAGC AATAAAAAAG 
AAGCTTGGAG AATTAACAGG GACAGTGAAG GAGAGTCTTG ATGAAGTCAG TAAGAGTGAC 
CTTGGCCGGA AGATCAAGGA GGGGGTAGAA GAGGCCGCCA GGACAGCCAA GCAGTCAGCG 
GAGTCAGTGT CCAAGAGCGG GGAGAAGCTG GGCAAGACGG CAGCCTTCAA AGCCATCTCC 
CAGGGTGTAG AGTCAGTGAA GAAGGAGCTT GACGAGAGCG TGCTTGGACA GACGGGGCCC 
TACCGCCGGC CCGAGCGGCT CCGGAAAAGG ACAGAGTTTG CTGGAGCAAA ATTCAAAGAA 
AGCAAAGTGT TCGAGGCTAA TGAGGAAGCA TTAGGGGTTG TGCTACACAA GGACTCCAAG 
TGGTACCAGC AGTGGAAGGA CTTCAAAGAT AACAACGTTG TATTCAACCG CTTCTTTGAG 
ATGAAGATGA AGTATGATGA AAGTGACAAT GTCCTCATCC GGGCGTCGCG GGCCTTAACC 
GACAAGGTCA CTGACTTGCT AGGAGGTCTT TTCTCAAAGA CAGAAATGTC AGAGGTGCTA 
ACGGAGATCC TGAGAGTGGA CCCAACCTTT GACAAGGACC ACTTTCTTCA TCAGTGTGAG 
ACTGACATCA TCCCCAACAT CCTAGAGGCC ATGATTTCCG GGGAGCTTGA CATTCTCAAA 
GACTGGTGCT ATGAAGCTAC CTACAGCCAG CTGGCCCACC CTATCCAGCA GGCCAAGGCT 
CTGGGCTTCC AGTTCCACTC CCGAATTCTG GACATCAGCA ATGTGGACCT GGCCATGGGC 
AAGATGATGG AGCAGGGCCC AGTGCTGATC GTTACCTTTC AGGCCCAGGT GGTGATGGTG 
ATCAAGAACT CCAAAGGCGA GGTGTATGAT GGTGACCCGG ACAAGGTGCA GCGAATGCTG 
TACGTGTGGG CACTCTGCAG AGACCAGGAG GAGCTCAACC CTTATGCTGC CTGGCGCCTT 
CTGGACATCT CAGCCTCCAG CACAGAGCAG ATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035722.2
CDS	19..1377
Translation	MAAAALRGGWCRCPRRCLGSGIQFLSSHNLPHGSSYQISRPGRELTLTKSYSSGSRKGFLSGLLDNIKQELAKNKEMKESIKKFRDEAKKLEESDALQEARRKYKSIESETVRTSEAIKKKLGELTGTVKESLDEVSKSDLGRKIKEGVEEAARTAKQSAESVSKSGEKLGKTAAFKAISQGVESVKKELDESVLGQTGPYRRPERLRKRTEFAGAKFKESKVFEANEEALGVVLHKDSKWYQQWKDFKDNNVVFNRFFEMKMKYDESDNVLIRASRALTDKVTDLLGGLFSKTEMSEVLTEILRVDPTFDKDHFLHQCETDIIPNILEAMISGELDILKDWCYEATYSQLAHPIQQAKALGFQFHSRILDISNVDLAMGKMMEQGPVLIVTFQAQVVMVIKNSKGEVYDGDPDKVQRMLYVWALCRDQEELNPYAAWRLLDISASSTEQIL

Target ORF information:

RefSeq Version	NM_011592.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus translocase of inner mitochondrial membrane 44 (Timm44), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011592.2

ORF Insert Sequence:

ATGGCGGCGG CAGCTCTGCG GGGTGGCTGG TGCCGCTGTC CGCGGAGGTG CCTAGGCAGC 
GGAATCCAAT TTCTCTCCAG CCACAACCTA CCCCATGGTT CAAGCTACCA GATAAGCCGG 
CCAGGAAGAG AGCTGACACT GACCAAATCT TATTCTTCTG GGAGCAGAAA AGGGTTTTTG 
TCAGGCTTGC TAGATAACAT CAAACAAGAA TTAGCCAAAA ACAAAGAGAT GAAAGAAAGT 
ATAAAAAAGT TCCGAGATGA GGCCAAGAAG CTAGAAGAGT CGGATGCTCT CCAGGAAGCC 
AGAAGGAAGT ATAAATCCAT CGAATCAGAA ACTGTGCGTA CCAGTGAAGC AATAAAAAAG 
AAGCTTGGAG AATTAACAGG GACAGTGAAG GAGAGTCTTG ATGAAGTCAG TAAGAGTGAC 
CTTGGCCGGA AGATCAAGGA GGGGGTAGAA GAGGCCGCCA GGACAGCCAA GCAGTCAGCG 
GAGTCAGTGT CCAAGAGCGG GGAGAAGCTG GGCAAGACGG CAGCCTTCAA AGCCATCTCC 
CAGGGTGTAG AGTCAGTGAA GAAGGAGCTT GACGAGAGCG TGCTTGGACA GACGGGGCCC 
TACCGCCGGC CCGAGCGGCT CCGGAAAAGG ACAGAGTTTG CTGGAGCAAA ATTCAAAGAA 
AGCAAAGTGT TCGAGGCTAA TGAGGAAGCA TTAGGGGTTG TGCTACACAA GGACTCCAAG 
TGGTACCAGC AGTGGAAGGA CTTCAAAGAT AACAACGTTG TATTCAACCG CTTCTTTGAG 
ATGAAGATGA AGTATGATGA AAGTGACAAT GTCCTCATCC GGGCGTCGCG GGCCTTAACC 
GACAAGGTCA CTGACTTGCT AGGAGGTCTT TTCTCAAAGA CAGAAATGTC AGAGGTGCTA 
ACGGAGATCC TGAGAGTGGA CCCAACCTTT GACAAGGACC ACTTTCTTCA TCAGTGTGAG 
ACTGACATCA TCCCCAACAT CCTAGAGGCC ATGATTTCCG GGGAGCTTGA CATTCTCAAA 
GACTGGTGCT ATGAAGCTAC CTACAGCCAG CTGGCCCACC CTATCCAGCA GGCCAAGGCT 
CTGGGCTTCC AGTTCCACTC CCGAATTCTG GACATCAGCA ATGTGGACCT GGCCATGGGC 
AAGATGATGG AGCAGGGCCC AGTGCTGATC GTTACCTTTC AGGCCCAGGT GGTGATGGTG 
ATCAAGAACT CCAAAGGCGA GGTGTATGAT GGTGACCCGG ACAAGGTGCA GCGAATGCTG 
TACGTGTGGG CACTCTGCAG AGACCAGGAG GAGCTCAACC CTTATGCTGC CTGGCGCCTT 
CTGGACATCT CAGCCTCCAG CACAGAGCAG ATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a fluorescent small-molecule enhancer for therapeutic autophagy in colorectal cancer by targeting mitochondrial protein translocase TIM44.
Gut67(2)307-319(2018 Feb)
Huang Y,Zhou J,Luo S,Wang Y,He J,Luo P,Chen Z,Liu T,Tan X,Ou J,Miao H,Liang H,Shi C

Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease.
Cell reports22(2)482-496(2018 Jan)
Fakruddin M,Wei FY,Suzuki T,Asano K,Kaieda T,Omori A,Izumi R,Fujimura A,Kaitsuka T,Miyata K,Araki K,Oike Y,Scorrano L,Suzuki T,Tomizawa K

Dynamics of mitochondrial DNA nucleoids regulated by mitochondrial fission is essential for maintenance of homogeneously active mitochondria during neonatal heart development.
Molecular and cellular biology35(1)211-23(2015 Jan)
Ishihara T,Ban-Ishihara R,Maeda M,Matsunaga Y,Ichimura A,Kyogoku S,Aoki H,Katada S,Nakada K,Nomura M,Mizushima N,Mihara K,Ishihara N

Mitochondrial fission factor Drp1 is essential for embryonic development and synapse formation in mice.
Nature cell biology11(8)958-66(2009 Aug)
Ishihara N,Nomura M,Jofuku A,Kato H,Suzuki SO,Masuda K,Otera H,Nakanishi Y,Nonaka I,Goto Y,Taguchi N,Morinaga H,Maeda M,Takayanagi R,Yokota S,Mihara K

Neurological phenotype and reduced lifespan in heterozygous Tim23 knockout mice, the first mouse model of defective mitochondrial import.
Biochimica et biophysica acta1787(5)371-6(2009 May)
Ahting U,Floss T,Uez N,Schneider-Lohmar I,Becker L,Kling E,Iuso A,Bender A,de Angelis MH,Gailus-Durner V,Fuchs H,Meitinger T,Wurst W,Prokisch H,Klopstock T

REQUEST A QUOTE

EMAIL

[email protected]

PHONE

1-877-436-7274

ONLINE FORM

Online Quote Submission

ONLINE FORM

Online Quote Submission

FAX

1-732-210-0262

Timm44 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

News & Blogs

REQUEST A QUOTE

EMAIL

PHONE

ONLINE FORM

ONLINE FORM

FAX