Irak1bp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Irak1bp1
Entrez Gene ID	65099
Full Name	interleukin-1 receptor-associated kinase 1 binding protein 1
Synonyms	4921528N06Rik,AI851240,Aabp3,Aip70,Simpl
General protein information	Preferred Names interleukin-1 receptor-associated kinase 1 binding protein 1 Names interleukin-1 receptor-associated kinase 1-binding protein 1 Acta binding protein 3 Acta-binding protein 70 IRAK1-binding protein 1 Plk-interacting protein signaling molecule that associates with the mouse pelle-like kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_022986.4	NP_075362.3	interleukin-1 receptor-associated kinase 1-binding protein 1 isoform 1
NM_001168240.1	NP_001161712.1	interleukin-1 receptor-associated kinase 1-binding protein 1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	IRAK1BP1	NP_001010844.1
Gallus gallus (chicken)	IRAK1BP1	NP_001264951.1
Pan troglodytes (chimpanzee)	IRAK1BP1	XP_518596.3
Canis lupus familiaris (dog)	IRAK1BP1	XP_539013.3
Bos taurus (cattle)	IRAK1BP1	NP_001103563.1
	IRAK1BP1	XP_001113735.1
Mus musculus (house mouse)	Irak1bp1	NP_075362.3
Rattus norvegicus (Norway rat)	Irak1bp1	NP_001264212.1
Danio rerio (zebrafish)	irak1bp1	NP_001018607.1
Xenopus tropicalis (tropical clawed frog)	irak1bp1	XP_002935125.1

Gene Ontology
Bibliography

Related articles in PubMed

SIMPL enhancement of tumor necrosis factor-? dependent p65-MED1 complex formation is required for mammalian hematopoietic stem and progenitor cell function.
Zhao W, Breese E, Bowers A, Hoggatt J, Pelus LM, Broxmeyer HE, Goebl M, Harrington MA
PloS one8(4)e61123(2013)

IRAK1BP1 inhibits inflammation by promoting nuclear translocation of NF-kappaB p50.
Conner JR, Smirnova II, Moseman AP, Poltorak A
Proceedings of the National Academy of Sciences of the United States of America107(25)11477-82(2010 Jun)

Loss of SIMPL compromises TNF-alpha-dependent survival of hematopoietic progenitors.
Benson EA, Goebl MG, Yang FC, Kapur R, McClintick J, Sanghani S, Clapp DW, Harrington MA
Experimental hematology38(2)71-81(2010 Feb)

Forward genetic analysis of Toll-like receptor responses in wild-derived mice reveals a novel antiinflammatory role for IRAK1BP1.
Conner JR, Smirnova II, Poltorak A
The Journal of experimental medicine205(2)305-14(2008 Feb)

The disordered amino-terminus of SIMPL interacts with members of the 70-kDa heat-shock protein family.
Haag Breese E, Uversky VN, Georgiadis MM, Harrington MA
DNA and cell biology25(12)704-14(2006 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SIMPL functions as a TNFalpha-dependent p65 co-activator by facilitating the recruitment of MED1 to p65 containing transcriptional complexes to control the expression of a subset of TNFalpha-induced genes.
Title: SIMPL enhancement of tumor necrosis factor-? dependent p65-MED1 complex formation is required for mammalian hematopoietic stem and progenitor cell function.

IRAK1BP1 functions through its effects on NF-kappaB as a molecular switch to bias innate immune pathways toward the resolution of inflammation
Title: IRAK1BP1 inhibits inflammation by promoting nuclear translocation of NF-kappaB p50.

Signals transduced through the tumor necrosis factor-alpha-tumor necrosis factor -receptor I-SIMPL(substrate that interacts with mouse pelle-like kinase) pathway support hematopoietic progenitor cell survival, growth and differentiation
Title: Loss of SIMPL compromises TNF-alpha-dependent survival of hematopoietic progenitors.

IRAK1BP1 regulates transcription of a specific subset of Toll-like receptor responsive genes, producing an overall antiinflammatory profile
Title: Forward genetic analysis of Toll-like receptor responses in wild-derived mice reveals a novel antiinflammatory role for IRAK1BP1.

Hsp70/Hsc70 bind the intrinsically disordered amino-terminal region of SIMPL to stabilize the protein and thereby regulate its activity.
Title: The disordered amino-terminus of SIMPL interacts with members of the 70-kDa heat-shock protein family.

The following Irak1bp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Irak1bp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13370	NM_001168240.1 Latest version!	Mus musculus interleukin-1 receptor-associated kinase 1 binding protein 1 (Irak1bp1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu13342	NM_022986.4 Latest version!	Mus musculus interleukin-1 receptor-associated kinase 1 binding protein 1 (Irak1bp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Irak1bp1 ( NM_001168240.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161712.1 Mus musculus interleukin-1 receptor-associated kinase 1 binding protein 1 (Irak1bp1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13370
Clone ID Related Accession (Same CDS sequence)	NM_001168240.1
Accession Version	NM_001168240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Mus musculus(house mouse)
Product	interleukin-1 receptor-associated kinase 1-binding protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY719456.1, AK014712.1 and AK045436.1. Transcript Variant: This variant (2) lacks an in-frame exon, compared to variant 1. This difference results in a shorter protein (isoform 2), compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK014712.1, BY714210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: lack of evidence for use of upstream AUG ##RefSeq-Attributes-END##

ATGTCGCTGC AACCCGCCCC TGCATCGCGG GTGTTCATGG AACTGGTTCC CTGGGCTGAC 
CGGGGCCGGG AAAACCATCC GATCTCGGCC GCGGAGGCGC AGCCGATCGG CCGCCGACCC 
CACGTCGCGG AGGCCCACCC CGGAGCCCGT GAGGTCCACG TGAGCGGCGC GGCCGAGGTG 
TCCGCCAGCC CCGACCGAGC GCTGGTGACC GTGCGAGTGA GCAGCACCAA GGAGGTGTCG 
GCCGAGGCCA AGAAGAGTGT GTGTCGCCGC CTGGACTACA TCACGCAGAG CCTCCAGCAG 
CAGGGGTTTC AGGTCTGCAT TACATTTACT GAATTTGGAA AAATGCAAAA TATTTGTAAC 
TTTCTGGTTG AAAAACTAGA TAGCTCTGTT GTCATCAGCC CACCTGAGTT CTATCACACA 
CCAGGTTCTG TTGAGAATCT TCGGCGGCAG GCCTGTCTGG TTGCTGTTGA GAATGCATGG 
CGCAAAGCTC AGGAAGTCTG TGACCTCGTT GGCCAAACTC TGGGAAAACC TTTACTAATC 
AAAGAAGAAG AAACAAAAGA CTGGGAAGGC CAAACGGATG ATCATCAGTT ATCCAGACTG 
CCAGGCACAT TAACTGTACA ACAGAAAATC AAAAGTGCAA CCATCCATGC AGCTTCGAAG 
GTGTTCATTA CTTTTGAGGT AAAAGGAAAA GAGAAGAAAA AAAAGCATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161712.1
CDS	223..936
Translation	MSLQPAPASRVFMELVPWADRGRENHPISAAEAQPIGRRPHVAEAHPGAREVHVSGAAEVSASPDRALVTVRVSSTKEVSAEAKKSVCRRLDYITQSLQQQGFQVCITFTEFGKMQNICNFLVEKLDSSVVISPPEFYHTPGSVENLRRQACLVAVENAWRKAQEVCDLVGQTLGKPLLIKEEETKDWEGQTDDHQLSRLPGTLTVQQKIKSATIHAASKVFITFEVKGKEKKKKHL

Target ORF information:

RefSeq Version	NM_001168240.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus interleukin-1 receptor-associated kinase 1 binding protein 1 (Irak1bp1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168240.1

ORF Insert Sequence:

ATGTCGCTGC AACCCGCCCC TGCATCGCGG GTGTTCATGG AACTGGTTCC CTGGGCTGAC 
CGGGGCCGGG AAAACCATCC GATCTCGGCC GCGGAGGCGC AGCCGATCGG CCGCCGACCC 
CACGTCGCGG AGGCCCACCC CGGAGCCCGT GAGGTCCACG TGAGCGGCGC GGCCGAGGTG 
TCCGCCAGCC CCGACCGAGC GCTGGTGACC GTGCGAGTGA GCAGCACCAA GGAGGTGTCG 
GCCGAGGCCA AGAAGAGTGT GTGTCGCCGC CTGGACTACA TCACGCAGAG CCTCCAGCAG 
CAGGGGTTTC AGGTCTGCAT TACATTTACT GAATTTGGAA AAATGCAAAA TATTTGTAAC 
TTTCTGGTTG AAAAACTAGA TAGCTCTGTT GTCATCAGCC CACCTGAGTT CTATCACACA 
CCAGGTTCTG TTGAGAATCT TCGGCGGCAG GCCTGTCTGG TTGCTGTTGA GAATGCATGG 
CGCAAAGCTC AGGAAGTCTG TGACCTCGTT GGCCAAACTC TGGGAAAACC TTTACTAATC 
AAAGAAGAAG AAACAAAAGA CTGGGAAGGC CAAACGGATG ATCATCAGTT ATCCAGACTG 
CCAGGCACAT TAACTGTACA ACAGAAAATC AAAAGTGCAA CCATCCATGC AGCTTCGAAG 
GTGTTCATTA CTTTTGAGGT AAAAGGAAAA GAGAAGAAAA AAAAGCATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irak1bp1 ( NM_022986.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075362.3 Mus musculus interleukin-1 receptor-associated kinase 1 binding protein 1 (Irak1bp1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13342
Clone ID Related Accession (Same CDS sequence)	NM_022986.4
Accession Version	NM_022986.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	interleukin-1 receptor-associated kinase 1-binding protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY719456.1 and AK045436.1. On Nov 24, 2009 this sequence version replaced NM_022986.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK045436.1, AK076594.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: lack of evidence for use of upstream AUG ##RefSeq-Attributes-END##

ATGTCGCTGC AACCCGCCCC TGCATCGCGG GTGTTCATGG AACTGGTTCC CTGGGCTGAC 
CGGGGCCGGG AAAACCATCC GATCTCGGCC GCGGAGGCGC AGCCGATCGG CCGCCGACCC 
CACGTCGCGG AGGCCCACCC CGGAGCCCGT GAGGTCCACG TGAGCGGCGC GGCCGAGGTG 
TCCGCCAGCC CCGACCGAGC GCTGGTGACC GTGCGAGTGA GCAGCACCAA GGAGGTGTCG 
GCCGAGGCCA AGAAGAGTGT GTGTCGCCGC CTGGACTACA TCACGCAGAG CCTCCAGCAG 
CAGGGGTTTC AGGCAGAAAA TGTAACTGTG ACAAAGAACA TTAGAAGAGT AGAAAATGCG 
TATCACATGG AAGCCGAGGT CTGCATTACA TTTACTGAAT TTGGAAAAAT GCAAAATATT 
TGTAACTTTC TGGTTGAAAA ACTAGATAGC TCTGTTGTCA TCAGCCCACC TGAGTTCTAT 
CACACACCAG GTTCTGTTGA GAATCTTCGG CGGCAGGCCT GTCTGGTTGC TGTTGAGAAT 
GCATGGCGCA AAGCTCAGGA AGTCTGTGAC CTCGTTGGCC AAACTCTGGG AAAACCTTTA 
CTAATCAAAG AAGAAGAAAC AAAAGACTGG GAAGGCCAAA CGGATGATCA TCAGTTATCC 
AGACTGCCAG GCACATTAAC TGTACAACAG AAAATCAAAA GTGCAACCAT CCATGCAGCT 
TCGAAGGTGT TCATTACTTT TGAGGTAAAA GGAAAAGAGA AGAAAAAAAA GCATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075362.3
CDS	223..1002
Translation	MSLQPAPASRVFMELVPWADRGRENHPISAAEAQPIGRRPHVAEAHPGAREVHVSGAAEVSASPDRALVTVRVSSTKEVSAEAKKSVCRRLDYITQSLQQQGFQAENVTVTKNIRRVENAYHMEAEVCITFTEFGKMQNICNFLVEKLDSSVVISPPEFYHTPGSVENLRRQACLVAVENAWRKAQEVCDLVGQTLGKPLLIKEEETKDWEGQTDDHQLSRLPGTLTVQQKIKSATIHAASKVFITFEVKGKEKKKKHL

Target ORF information:

RefSeq Version	NM_022986.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus interleukin-1 receptor-associated kinase 1 binding protein 1 (Irak1bp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022986.4

ORF Insert Sequence:

ATGTCGCTGC AACCCGCCCC TGCATCGCGG GTGTTCATGG AACTGGTTCC CTGGGCTGAC 
CGGGGCCGGG AAAACCATCC GATCTCGGCC GCGGAGGCGC AGCCGATCGG CCGCCGACCC 
CACGTCGCGG AGGCCCACCC CGGAGCCCGT GAGGTCCACG TGAGCGGCGC GGCCGAGGTG 
TCCGCCAGCC CCGACCGAGC GCTGGTGACC GTGCGAGTGA GCAGCACCAA GGAGGTGTCG 
GCCGAGGCCA AGAAGAGTGT GTGTCGCCGC CTGGACTACA TCACGCAGAG CCTCCAGCAG 
CAGGGGTTTC AGGCAGAAAA TGTAACTGTG ACAAAGAACA TTAGAAGAGT AGAAAATGCG 
TATCACATGG AAGCCGAGGT CTGCATTACA TTTACTGAAT TTGGAAAAAT GCAAAATATT 
TGTAACTTTC TGGTTGAAAA ACTAGATAGC TCTGTTGTCA TCAGCCCACC TGAGTTCTAT 
CACACACCAG GTTCTGTTGA GAATCTTCGG CGGCAGGCCT GTCTGGTTGC TGTTGAGAAT 
GCATGGCGCA AAGCTCAGGA AGTCTGTGAC CTCGTTGGCC AAACTCTGGG AAAACCTTTA 
CTAATCAAAG AAGAAGAAAC AAAAGACTGG GAAGGCCAAA CGGATGATCA TCAGTTATCC 
AGACTGCCAG GCACATTAAC TGTACAACAG AAAATCAAAA GTGCAACCAT CCATGCAGCT 
TCGAAGGTGT TCATTACTTT TGAGGTAAAA GGAAAAGAGA AGAAAAAAAA GCATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SIMPL enhancement of tumor necrosis factor-? dependent p65-MED1 complex formation is required for mammalian hematopoietic stem and progenitor cell function.
PloS one8(4)e61123(2013)
Zhao W,Breese E,Bowers A,Hoggatt J,Pelus LM,Broxmeyer HE,Goebl M,Harrington MA

IRAK1BP1 inhibits inflammation by promoting nuclear translocation of NF-kappaB p50.
Proceedings of the National Academy of Sciences of the United States of America107(25)11477-82(2010 Jun)
Conner JR,Smirnova II,Moseman AP,Poltorak A

Loss of SIMPL compromises TNF-alpha-dependent survival of hematopoietic progenitors.
Experimental hematology38(2)71-81(2010 Feb)
Benson EA,Goebl MG,Yang FC,Kapur R,McClintick J,Sanghani S,Clapp DW,Harrington MA

Forward genetic analysis of Toll-like receptor responses in wild-derived mice reveals a novel antiinflammatory role for IRAK1BP1.
The Journal of experimental medicine205(2)305-14(2008 Feb)
Conner JR,Smirnova II,Poltorak A

The disordered amino-terminus of SIMPL interacts with members of the 70-kDa heat-shock protein family.
DNA and cell biology25(12)704-14(2006 Dec)
Haag Breese E,Uversky VN,Georgiadis MM,Harrington MA