Trnp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trnp1
Entrez Gene ID	69539
Full Name	TMF1-regulated nuclear protein 1
Synonyms	2300002D11Rik,Trnp
General protein information	Preferred Names TMF1-regulated nuclear protein 1 Names TMF-regulated nuclear protein 1 mexneurin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006539155.1	XP_006539218.1	TMF-regulated nuclear protein 1 isoform X1
XM_006539155.2	XP_006539218.1	TMF-regulated nuclear protein 1 isoform X1
XM_006539155.3	XP_006539218.1	TMF-regulated nuclear protein 1 isoform X1
NM_001081156.2	NP_001074625.1	TMF-regulated nuclear protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TRNP1	XP_003307956.1
Bos taurus (cattle)	TRNP1	XP_003585856.1
Homo sapiens (human)	TRNP1	NP_001013664.1
	TRNP1	XP_001110675.1
Mus musculus (house mouse)	Trnp1	NP_001074625.1
Rattus norvegicus (Norway rat)	Trnp1	XP_001069068.4

Gene Ontology
Bibliography

Related articles in PubMed

Mexneurin is a novel precursor of peptides in the central nervous system of rodents.
Matus-Ortega ME, Leff Gelman P, Calva-Nieves JC, Flores-Zamora A, Salazar-Ju?rez A, Torner-Aguilar CA, Gamba G, De Los Heros P, Peng B, Pintar JE, Gompf HS, Allen CN, Ant?n-Palma B
FEBS letters591(12)1627-1636(2017 Jun)

Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina.
Park KU, Randazzo G, Jones KL, Brzezinski JA 4th
Investigative ophthalmology & visual science58(2)1137-1150(2017 02)

Discrete domains of gene expression in germinal layers distinguish the development of gyrencephaly.
de Juan Romero C, Bruder C, Tomasello U, Sanz-Anquela JM, Borrell V
The EMBO journal34(14)1859-74(2015 Jul)

Trnp1 regulates expansion and folding of the mammalian cerebral cortex by control of radial glial fate.
Stahl R, Walcher T, De Juan Romero C, Pilz GA, Cappello S, Irmler M, Sanz-Aquela JM, Beckers J, Blum R, Borrell V, G?tz M
Cell153(3)535-49(2013 Apr)

Ezh2, the histone methyltransferase of PRC2, regulates the balance between self-renewal and differentiation in the cerebral cortex.
Pereira JD, Sansom SN, Smith J, Dobenecker MW, Tarakhovsky A, Livesey FJ
Proceedings of the National Academy of Sciences of the United States of America107(36)15957-62(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that neurons in mouse brain exhibit DNA sequence encoding protein precursor, proMexneurin, that contains three peptide sequences: Mexneurin-1 (an endomorphin1-like peptide), Mexneurin-2 (an endomorphin1-like peptide), and Mexneurin-3 (peptide which appears to be unrelated to endomorphins); neurons exhibit expression of proMexneurin mRNA throughout mouse brain.
Title: Mexneurin is a novel precursor of peptides in the central nervous system of rodents.

Study identified Trnp1 as a regulator of mammalian brain development and as a key factor controlling neocortical expansion. Trnp1 levels have striking effects on tangential, radial, and lateral expansion of the cerebral cortex.
Title: Trnp1 regulates expansion and folding of the mammalian cerebral cortex by control of radial glial fate.

The following Trnp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trnp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15070	NM_001081156.2 Latest version!	Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu15070	XM_006539155.3 Latest version!	Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu15070	XM_006539155.1	Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu15070	XM_006539155.2	Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trnp1 ( NM_001081156.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074625.1 Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), mRNA.

Price & Availability↑

CloneID	OMu15070
Clone ID Related Accession (Same CDS sequence)	XM_006539155.1 , XM_006539155.2 , XM_006539155.3 , NM_001081156.2
Accession Version	NM_001081156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	TMF-regulated nuclear protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB579578.1, BC049168.1 and AW120970.1. On Apr 11, 2007 this sequence version replaced NM_001081156.1. CCDS Note: This CCDS ID represents the protein described in PMID: 16792503 and is supported by the mRNA BC049168.1. It should be noted that this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 16792503. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. ##Evidence-Data-START## Transcript exon combination :: BC049168.1, AY142705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 16792503 ##RefSeq-Attributes-END##

ATGCCGGGCT GCCGAATCAG CGCCTGCGGC CCAGGGGCCC AGGAAGGGAC GGCAGAACCG 
GGGTCGCCCC CGCCGCCACC CCGGGAGCCC CTGCCGTCCC TCCAGCCCCC TTCCCCATCG 
CCGACCTCGA CTCCGACCCC CACTAAGTCA CCGCCCTTAC CCGAGGCGGC CGAGACACCC 
GTGGAAGGGC AGGAGCTGCA GCGCTGGCGC CAGGGTGCTA GTGGGGGGTC TGGGGGCGCG 
GGCCCGGCGG GGATCGCGGG CGCGGCGGCG GGGGCAGGGG GCCGTGCGCT GGAGCTGGCC 
GAAGCGCGGC GGCGACTGCT AGAAGTGGAG GGCCGCCGGC GCCTGGTGTC GGAGCTGGAG 
AGCCGGGTGC TGCAGCTGCA CCGAGTCTTC TTGGCTGCGG AGCTGCGCCT GGCGCATCGC 
GCCGAGAGCC TGAGCCGCCT GAGCGGCGGC GTGGCCCAGG CCGAGCTCTA CCTGGCTGCG 
CACGGGTCCC GTCTCAAGAA GGGTGCGCGC CGCGGTCGCC GGGGTCGTCC CCCGGCGCTG 
CTGGCGTCCG CGCTCGGCCT GGGGAGCTGC GTGCCCTGGG GCGCAGGGCG GCTACGGCGC 
GGCCAGGGCC CGGAGCCCGA CTCGCCCTTC CGCCGCAGCC CGCCCCGCGG CCCCGCCTCC 
CCCCAGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074625.1
CDS	94..765
Translation	MPGCRISACGPGAQEGTAEPGSPPPPPREPLPSLQPPSPSPTSTPTPTKSPPLPEAAETPVEGQELQRWRQGASGGSGGAGPAGIAGAAAGAGGRALELAEARRRLLEVEGRRRLVSELESRVLQLHRVFLAAELRLAHRAESLSRLSGGVAQAELYLAAHGSRLKKGARRGRRGRPPALLASALGLGSCVPWGAGRLRRGQGPEPDSPFRRSPPRGPASPQR

Target ORF information:

RefSeq Version	NM_001081156.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081156.2

ORF Insert Sequence:

ATGCCGGGCT GCCGAATCAG CGCCTGCGGC CCAGGGGCCC AGGAAGGGAC GGCAGAACCG 
GGGTCGCCCC CGCCGCCACC CCGGGAGCCC CTGCCGTCCC TCCAGCCCCC TTCCCCATCG 
CCGACCTCGA CTCCGACCCC CACTAAGTCA CCGCCCTTAC CCGAGGCGGC CGAGACACCC 
GTGGAAGGGC AGGAGCTGCA GCGCTGGCGC CAGGGTGCTA GTGGGGGGTC TGGGGGCGCG 
GGCCCGGCGG GGATCGCGGG CGCGGCGGCG GGGGCAGGGG GCCGTGCGCT GGAGCTGGCC 
GAAGCGCGGC GGCGACTGCT AGAAGTGGAG GGCCGCCGGC GCCTGGTGTC GGAGCTGGAG 
AGCCGGGTGC TGCAGCTGCA CCGAGTCTTC TTGGCTGCGG AGCTGCGCCT GGCGCATCGC 
GCCGAGAGCC TGAGCCGCCT GAGCGGCGGC GTGGCCCAGG CCGAGCTCTA CCTGGCTGCG 
CACGGGTCCC GTCTCAAGAA GGGTGCGCGC CGCGGTCGCC GGGGTCGTCC CCCGGCGCTG 
CTGGCGTCCG CGCTCGGCCT GGGGAGCTGC GTGCCCTGGG GCGCAGGGCG GCTACGGCGC 
GGCCAGGGCC CGGAGCCCGA CTCGCCCTTC CGCCGCAGCC CGCCCCGCGG CCCCGCCTCC 
CCCCAGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trnp1 ( XM_006539155.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539218.1 Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15070
Clone ID Related Accession (Same CDS sequence)	XM_006539155.1 , XM_006539155.2 , XM_006539155.3 , NM_001081156.2
Accession Version	XM_006539155.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	TMF-regulated nuclear protein 1 isoform X1
Comment

ATGCCGGGCT GCCGAATCAG CGCCTGCGGC CCAGGGGCCC AGGAAGGGAC GGCAGAACCG 
GGGTCGCCCC CGCCGCCACC CCGGGAGCCC CTGCCGTCCC TCCAGCCCCC TTCCCCATCG 
CCGACCTCGA CTCCGACCCC CACTAAGTCA CCGCCCTTAC CCGAGGCGGC CGAGACACCC 
GTGGAAGGGC AGGAGCTGCA GCGCTGGCGC CAGGGTGCTA GTGGGGGGTC TGGGGGCGCG 
GGCCCGGCGG GGATCGCGGG CGCGGCGGCG GGGGCAGGGG GCCGTGCGCT GGAGCTGGCC 
GAAGCGCGGC GGCGACTGCT AGAAGTGGAG GGCCGCCGGC GCCTGGTGTC GGAGCTGGAG 
AGCCGGGTGC TGCAGCTGCA CCGAGTCTTC TTGGCTGCGG AGCTGCGCCT GGCGCATCGC 
GCCGAGAGCC TGAGCCGCCT GAGCGGCGGC GTGGCCCAGG CCGAGCTCTA CCTGGCTGCG 
CACGGGTCCC GTCTCAAGAA GGGTGCGCGC CGCGGTCGCC GGGGTCGTCC CCCGGCGCTG 
CTGGCGTCCG CGCTCGGCCT GGGGAGCTGC GTGCCCTGGG GCGCAGGGCG GCTACGGCGC 
GGCCAGGGCC CGGAGCCCGA CTCGCCCTTC CGCCGCAGCC CGCCCCGCGG CCCCGCCTCC 
CCCCAGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539218.1
Translation	MPGCRISACGPGAQEGTAEPGSPPPPPREPLPSLQPPSPSPTSTPTPTKSPPLPEAAETPVEGQELQRWRQGASGGSGGAGPAGIAGAAAGAGGRALELAEARRRLLEVEGRRRLVSELESRVLQLHRVFLAAELRLAHRAESLSRLSGGVAQAELYLAAHGSRLKKGARRGRRGRPPALLASALGLGSCVPWGAGRLRRGQGPEPDSPFRRSPPRGPASPQR

Target ORF information:

RefSeq Version	XM_006539155.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539155.1

ORF Insert Sequence:

ATGCCGGGCT GCCGAATCAG CGCCTGCGGC CCAGGGGCCC AGGAAGGGAC GGCAGAACCG 
GGGTCGCCCC CGCCGCCACC CCGGGAGCCC CTGCCGTCCC TCCAGCCCCC TTCCCCATCG 
CCGACCTCGA CTCCGACCCC CACTAAGTCA CCGCCCTTAC CCGAGGCGGC CGAGACACCC 
GTGGAAGGGC AGGAGCTGCA GCGCTGGCGC CAGGGTGCTA GTGGGGGGTC TGGGGGCGCG 
GGCCCGGCGG GGATCGCGGG CGCGGCGGCG GGGGCAGGGG GCCGTGCGCT GGAGCTGGCC 
GAAGCGCGGC GGCGACTGCT AGAAGTGGAG GGCCGCCGGC GCCTGGTGTC GGAGCTGGAG 
AGCCGGGTGC TGCAGCTGCA CCGAGTCTTC TTGGCTGCGG AGCTGCGCCT GGCGCATCGC 
GCCGAGAGCC TGAGCCGCCT GAGCGGCGGC GTGGCCCAGG CCGAGCTCTA CCTGGCTGCG 
CACGGGTCCC GTCTCAAGAA GGGTGCGCGC CGCGGTCGCC GGGGTCGTCC CCCGGCGCTG 
CTGGCGTCCG CGCTCGGCCT GGGGAGCTGC GTGCCCTGGG GCGCAGGGCG GCTACGGCGC 
GGCCAGGGCC CGGAGCCCGA CTCGCCCTTC CGCCGCAGCC CGCCCCGCGG CCCCGCCTCC 
CCCCAGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trnp1 ( XM_006539155.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539218.1 Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15070
Clone ID Related Accession (Same CDS sequence)	XM_006539155.1 , XM_006539155.2 , XM_006539155.3 , NM_001081156.2
Accession Version	XM_006539155.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	TMF-regulated nuclear protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539155.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCT GCCGAATCAG CGCCTGCGGC CCAGGGGCCC AGGAAGGGAC GGCAGAACCG 
GGGTCGCCCC CGCCGCCACC CCGGGAGCCC CTGCCGTCCC TCCAGCCCCC TTCCCCATCG 
CCGACCTCGA CTCCGACCCC CACTAAGTCA CCGCCCTTAC CCGAGGCGGC CGAGACACCC 
GTGGAAGGGC AGGAGCTGCA GCGCTGGCGC CAGGGTGCTA GTGGGGGGTC TGGGGGCGCG 
GGCCCGGCGG GGATCGCGGG CGCGGCGGCG GGGGCAGGGG GCCGTGCGCT GGAGCTGGCC 
GAAGCGCGGC GGCGACTGCT AGAAGTGGAG GGCCGCCGGC GCCTGGTGTC GGAGCTGGAG 
AGCCGGGTGC TGCAGCTGCA CCGAGTCTTC TTGGCTGCGG AGCTGCGCCT GGCGCATCGC 
GCCGAGAGCC TGAGCCGCCT GAGCGGCGGC GTGGCCCAGG CCGAGCTCTA CCTGGCTGCG 
CACGGGTCCC GTCTCAAGAA GGGTGCGCGC CGCGGTCGCC GGGGTCGTCC CCCGGCGCTG 
CTGGCGTCCG CGCTCGGCCT GGGGAGCTGC GTGCCCTGGG GCGCAGGGCG GCTACGGCGC 
GGCCAGGGCC CGGAGCCCGA CTCGCCCTTC CGCCGCAGCC CGCCCCGCGG CCCCGCCTCC 
CCCCAGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539218.1
CDS	769..1440
Translation	MPGCRISACGPGAQEGTAEPGSPPPPPREPLPSLQPPSPSPTSTPTPTKSPPLPEAAETPVEGQELQRWRQGASGGSGGAGPAGIAGAAAGAGGRALELAEARRRLLEVEGRRRLVSELESRVLQLHRVFLAAELRLAHRAESLSRLSGGVAQAELYLAAHGSRLKKGARRGRRGRPPALLASALGLGSCVPWGAGRLRRGQGPEPDSPFRRSPPRGPASPQR

Target ORF information:

RefSeq Version	XM_006539155.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539155.2

ORF Insert Sequence:

ATGCCGGGCT GCCGAATCAG CGCCTGCGGC CCAGGGGCCC AGGAAGGGAC GGCAGAACCG 
GGGTCGCCCC CGCCGCCACC CCGGGAGCCC CTGCCGTCCC TCCAGCCCCC TTCCCCATCG 
CCGACCTCGA CTCCGACCCC CACTAAGTCA CCGCCCTTAC CCGAGGCGGC CGAGACACCC 
GTGGAAGGGC AGGAGCTGCA GCGCTGGCGC CAGGGTGCTA GTGGGGGGTC TGGGGGCGCG 
GGCCCGGCGG GGATCGCGGG CGCGGCGGCG GGGGCAGGGG GCCGTGCGCT GGAGCTGGCC 
GAAGCGCGGC GGCGACTGCT AGAAGTGGAG GGCCGCCGGC GCCTGGTGTC GGAGCTGGAG 
AGCCGGGTGC TGCAGCTGCA CCGAGTCTTC TTGGCTGCGG AGCTGCGCCT GGCGCATCGC 
GCCGAGAGCC TGAGCCGCCT GAGCGGCGGC GTGGCCCAGG CCGAGCTCTA CCTGGCTGCG 
CACGGGTCCC GTCTCAAGAA GGGTGCGCGC CGCGGTCGCC GGGGTCGTCC CCCGGCGCTG 
CTGGCGTCCG CGCTCGGCCT GGGGAGCTGC GTGCCCTGGG GCGCAGGGCG GCTACGGCGC 
GGCCAGGGCC CGGAGCCCGA CTCGCCCTTC CGCCGCAGCC CGCCCCGCGG CCCCGCCTCC 
CCCCAGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trnp1 ( XM_006539155.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539218.1 Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu15070
Clone ID Related Accession (Same CDS sequence)	XM_006539155.1 , XM_006539155.2 , XM_006539155.3 , NM_001081156.2
Accession Version	XM_006539155.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	TMF-regulated nuclear protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539155.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGCT GCCGAATCAG CGCCTGCGGC CCAGGGGCCC AGGAAGGGAC GGCAGAACCG 
GGGTCGCCCC CGCCGCCACC CCGGGAGCCC CTGCCGTCCC TCCAGCCCCC TTCCCCATCG 
CCGACCTCGA CTCCGACCCC CACTAAGTCA CCGCCCTTAC CCGAGGCGGC CGAGACACCC 
GTGGAAGGGC AGGAGCTGCA GCGCTGGCGC CAGGGTGCTA GTGGGGGGTC TGGGGGCGCG 
GGCCCGGCGG GGATCGCGGG CGCGGCGGCG GGGGCAGGGG GCCGTGCGCT GGAGCTGGCC 
GAAGCGCGGC GGCGACTGCT AGAAGTGGAG GGCCGCCGGC GCCTGGTGTC GGAGCTGGAG 
AGCCGGGTGC TGCAGCTGCA CCGAGTCTTC TTGGCTGCGG AGCTGCGCCT GGCGCATCGC 
GCCGAGAGCC TGAGCCGCCT GAGCGGCGGC GTGGCCCAGG CCGAGCTCTA CCTGGCTGCG 
CACGGGTCCC GTCTCAAGAA GGGTGCGCGC CGCGGTCGCC GGGGTCGTCC CCCGGCGCTG 
CTGGCGTCCG CGCTCGGCCT GGGGAGCTGC GTGCCCTGGG GCGCAGGGCG GCTACGGCGC 
GGCCAGGGCC CGGAGCCCGA CTCGCCCTTC CGCCGCAGCC CGCCCCGCGG CCCCGCCTCC 
CCCCAGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539218.1
CDS	1189..1860
Translation	MPGCRISACGPGAQEGTAEPGSPPPPPREPLPSLQPPSPSPTSTPTPTKSPPLPEAAETPVEGQELQRWRQGASGGSGGAGPAGIAGAAAGAGGRALELAEARRRLLEVEGRRRLVSELESRVLQLHRVFLAAELRLAHRAESLSRLSGGVAQAELYLAAHGSRLKKGARRGRRGRPPALLASALGLGSCVPWGAGRLRRGQGPEPDSPFRRSPPRGPASPQR

Target ORF information:

RefSeq Version	XM_006539155.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus TMF1-regulated nuclear protein 1 (Trnp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539155.3

ORF Insert Sequence:

ATGCCGGGCT GCCGAATCAG CGCCTGCGGC CCAGGGGCCC AGGAAGGGAC GGCAGAACCG 
GGGTCGCCCC CGCCGCCACC CCGGGAGCCC CTGCCGTCCC TCCAGCCCCC TTCCCCATCG 
CCGACCTCGA CTCCGACCCC CACTAAGTCA CCGCCCTTAC CCGAGGCGGC CGAGACACCC 
GTGGAAGGGC AGGAGCTGCA GCGCTGGCGC CAGGGTGCTA GTGGGGGGTC TGGGGGCGCG 
GGCCCGGCGG GGATCGCGGG CGCGGCGGCG GGGGCAGGGG GCCGTGCGCT GGAGCTGGCC 
GAAGCGCGGC GGCGACTGCT AGAAGTGGAG GGCCGCCGGC GCCTGGTGTC GGAGCTGGAG 
AGCCGGGTGC TGCAGCTGCA CCGAGTCTTC TTGGCTGCGG AGCTGCGCCT GGCGCATCGC 
GCCGAGAGCC TGAGCCGCCT GAGCGGCGGC GTGGCCCAGG CCGAGCTCTA CCTGGCTGCG 
CACGGGTCCC GTCTCAAGAA GGGTGCGCGC CGCGGTCGCC GGGGTCGTCC CCCGGCGCTG 
CTGGCGTCCG CGCTCGGCCT GGGGAGCTGC GTGCCCTGGG GCGCAGGGCG GCTACGGCGC 
GGCCAGGGCC CGGAGCCCGA CTCGCCCTTC CGCCGCAGCC CGCCCCGCGG CCCCGCCTCC 
CCCCAGCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mexneurin is a novel precursor of peptides in the central nervous system of rodents.
FEBS letters591(12)1627-1636(2017 Jun)
Matus-Ortega ME,Leff Gelman P,Calva-Nieves JC,Flores-Zamora A,Salazar-Ju?rez A,Torner-Aguilar CA,Gamba G,De Los Heros P,Peng B,Pintar JE,Gompf HS,Allen CN,Ant?n-Palma B

Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina.
Investigative ophthalmology & visual science58(2)1137-1150(2017 02)
Park KU,Randazzo G,Jones KL,Brzezinski JA 4th

Discrete domains of gene expression in germinal layers distinguish the development of gyrencephaly.
The EMBO journal34(14)1859-74(2015 Jul)
de Juan Romero C,Bruder C,Tomasello U,Sanz-Anquela JM,Borrell V

Trnp1 regulates expansion and folding of the mammalian cerebral cortex by control of radial glial fate.
Cell153(3)535-49(2013 Apr)
Stahl R,Walcher T,De Juan Romero C,Pilz GA,Cappello S,Irmler M,Sanz-Aquela JM,Beckers J,Blum R,Borrell V,G?tz M

Ezh2, the histone methyltransferase of PRC2, regulates the balance between self-renewal and differentiation in the cerebral cortex.
Proceedings of the National Academy of Sciences of the United States of America107(36)15957-62(2010 Sep)
Pereira JD,Sansom SN,Smith J,Dobenecker MW,Tarakhovsky A,Livesey FJ

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Trnp1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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