Pold4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Gene Symbol	Pold4
Entrez Gene ID	69745
Full Name	polymerase (DNA-directed), delta 4
Synonyms	2410012M21Rik,AI463381,AW060307,Polds,p12
General protein information	Preferred Names polymerase (DNA-directed), delta 4 Names DNA polymerase delta subunit 4 DNA polymerase delta smallest subunit p12
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA Protein Name

NM_027196.4 NP_081472.1 DNA polymerase delta subunit 4
Pathways from BioSystems

mRNA	Protein	Name
NM_027196.4	NP_081472.1	DNA polymerase delta subunit 4

Homologies

	POLD4	XP_001107074.1
Rattus norvegicus (Norway rat)	Pold4	NP_001013213.1
Pan troglodytes (chimpanzee)	POLD4	XP_001173013.1
Canis lupus familiaris (dog)	POLD4	XP_854661.1
Homo sapiens (human)	POLD4	NP_066996.3
Mus musculus (house mouse)	Pold4	NP_081472.1
Bos taurus (cattle)	POLD4	NP_001069949.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase ? for degradation in response to DNA damage.
Zhang S, Zhou Y, Sarkeshik A, Yates JR 3rd, Thomson TM, Zhang Z, Lee EY, Lee MY
The Journal of biological chemistry288(5)2941-50(2013 Feb)

FGF2-induced upregulation of DNA polymerase-delta p12 subunit in endothelial cells.
Dell'Era P, Nicoli S, Peri G, Nieddu M, Ennas MG, Presta M
Oncogene24(6)1117-21(2005 Feb)

Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set.
VanBuren V, Piao Y, Dudekula DB, Qian Y, Carter MG, Martin PR, Stagg CA, Bassey UC, Aiba K, Hamatani T, Kargul GJ, Luo AG, Kelso J, Hide W, Ko MS
Genome research12(12)1999-2003(2002 Dec)

Gene expression profile in fibroblast growth factor 2-transformed endothelial cells.
Dell'Era P, Coco L, Ronca R, Sennino B, Presta M
Oncogene21(15)2433-40(2002 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The identification of RNF8 allows new insights into the integration of the control of p12 degradation by different DNA damage signaling pathways.
Title: Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase ? for degradation in response to DNA damage.

The following Pold4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pold4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13535	NM_027196.4 Latest version!	Mus musculus polymerase (DNA-directed), delta 4 (Pold4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pold4 ( NM_027196.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081472.1 Mus musculus polymerase (DNA-directed), delta 4 (Pold4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13535
Clone ID Related Accession (Same CDS sequence)	NM_027196.4
Accession Version	NM_027196.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-11
Organism	Mus musculus(house mouse)
Product	DNA polymerase delta subunit 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC109138.10 and BC028520.1. On Jun 20, 2015 this sequence version replaced NM_027196.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: BY709901.1, CB194474.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCGGA AGCGGTTCAT CACTGACTCC TATCCTGTTG TGAAGAAGAG GGAGGGGCCC 
CCTGGGCACA GCAAGGGAGA GCTGGCACCC GAGCTAGGGG AAGACACCCA GTCCCTCAGC 
CAGGAGGAAA CAGAGCTGGA GCTGCTGAGG CAGTTTGACC TGGCCTGGCA GTATGGGCCT 
TGTACAGGTA TCACAAGGCT GCAGCGCTGG AGTCGGGCAG AGCAGATGGG CTTGAAGCCC 
CCCCTAGAGG TGTACCAAGT GTTGAAGGCA CACCCTGAAG ACCCTCACTT CCAATGCAGC 
CTGTGGCATC TCTACCCACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081472.1
CDS	200..523
Translation	MGRKRFITDSYPVVKKREGPPGHSKGELAPELGEDTQSLSQEETELELLRQFDLAWQYGPCTGITRLQRWSRAEQMGLKPPLEVYQVLKAHPEDPHFQCSLWHLYPL

Target ORF information:

RefSeq Version	NM_027196.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus polymerase (DNA-directed), delta 4 (Pold4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027196.4

ORF Insert Sequence:

ATGGGTCGGA AGCGGTTCAT CACTGACTCC TATCCTGTTG TGAAGAAGAG GGAGGGGCCC 
CCTGGGCACA GCAAGGGAGA GCTGGCACCC GAGCTAGGGG AAGACACCCA GTCCCTCAGC 
CAGGAGGAAA CAGAGCTGGA GCTGCTGAGG CAGTTTGACC TGGCCTGGCA GTATGGGCCT 
TGTACAGGTA TCACAAGGCT GCAGCGCTGG AGTCGGGCAG AGCAGATGGG CTTGAAGCCC 
CCCCTAGAGG TGTACCAAGT GTTGAAGGCA CACCCTGAAG ACCCTCACTT CCAATGCAGC 
CTGTGGCATC TCTACCCACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of RNF8 as a ubiquitin ligase involved in targeting the p12 subunit of DNA polymerase ? for degradation in response to DNA damage.
The Journal of biological chemistry288(5)2941-50(2013 Feb)
Zhang S,Zhou Y,Sarkeshik A,Yates JR 3rd,Thomson TM,Zhang Z,Lee EY,Lee MY

FGF2-induced upregulation of DNA polymerase-delta p12 subunit in endothelial cells.
Oncogene24(6)1117-21(2005 Feb)
Dell'Era P,Nicoli S,Peri G,Nieddu M,Ennas MG,Presta M

Assembly, verification, and initial annotation of the NIA mouse 7.4K cDNA clone set.
Genome research12(12)1999-2003(2002 Dec)
VanBuren V,Piao Y,Dudekula DB,Qian Y,Carter MG,Martin PR,Stagg CA,Bassey UC,Aiba K,Hamatani T,Kargul GJ,Luo AG,Kelso J,Hide W,Ko MS

Gene expression profile in fibroblast growth factor 2-transformed endothelial cells.
Oncogene21(15)2433-40(2002 Apr)
Dell'Era P,Coco L,Ronca R,Sennino B,Presta M

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Pold4 cDNA ORF clone, Mus musculus(house mouse)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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