Overview

The quest to characterize novel proteins can be elusive. For many researchers working in specialized model organisms such as insects, fish, worms, parasites, and plants, purchasing pre-made antibodies just simply isn’t an option.

Custom polyclonal antibodies allow you to specify, validate, and visualize specific protein targets

  • In a research study conducted by Kolosov et al., generating three custom polyclonal antibodies (pAbs) presented an ideal solution to the problem of detecting and characterizing novel proteins. Since pAbs recognize multiple epitopes of the same target protein, developing his own custom antibodies using GenScript’s popular pAb service maximized success of detecting the proteins of interest.

Uncovering how sea lamprey adapt to survive in habitats of varying salinity levels

  • Sea lamprey (Petromyzon marinus) is a parasitic fish that is able to survive in waters that vary widely in salinity levels. Post-metamorphosis, mature P. marinus reside primarily in seawater before returning to freshwater habitats to reproduce. As larvae, however, P. marinus can only tolerate living in freshwater within a strictly limited salinity range. As a result, this adaptation for surviving in aquatic habitats of varying ionic strength depends greatly on the stage of this organism’s life cycle.
  • To understand the molecular physiology behind P. marinus’ unique osmo/ionoregulation ability that enabled these fish to maintain stable serum ion levels while residing in habitats of varying ionic strength. Their research focused on the tight junction (TJ) proteins in larval sea lamprey that are responsible for paracellular solute movement across tissue-water barriers.

    Scale bars: (B) 50 μm, (C–J) 25 μm. BV, blood vessel; Ext, exterior; Lu, lumen; Per, peritoneal side.

Figure reproduced with permission from the Journal of Experimental Biology (doi:10.1242/jeb.161562), with additional consent from the study’s author Dennis Kolosov, Ph.D. (www.denniskolosov.com).

Why Custom Polyclonal Antibodies?

Using pAbs maximizes your chances of specific protein detection across classic model organisms (Drosophila, Xenopus, C. elegans, zebrafish, Arabidopsis, etc.) as well as in rare animal and plant species! In addition, pAb production is fast, straightforward, and affordable, ensuring the best bang for your buck!

See where else our custom pAbs have been used by exploring some of our selected publications! Explore additional model organisms such as parasites as well as other animal and plant species using our citations database.

Insects
Worms
Fish
Plants
  • Konstantinides, N. et al. Phenotypic Convergence: Distinct Transcription Factors Regulate Common Terminal Features. Cell (2018). Link
  • Zhang, M. et al. Brv1 Is Required for Drosophila Larvae to Sense Gentle Touch. Cell Rep. (2018). Link
  • Isoe, J. et al. Xanthine dehydrogenase-1 silencing in Aedes aegypti mosquitoes promotes a blood feeding-induced adulticidal activity. FASEB J. (2017). Link
  • Ferrandiz, N. et al. Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. Elegans oocyte meiosis. Nat. Commun. (2018). Link
  • Nadarajan, S. et al. Polo-like kinase-dependent phosphorylation of the synaptonemal complex protein SYP-4 regulates double-strand break formation through a negative feedback loop. Elife (2017). Link
  • Balasubramaniam, M., Ayyadevara, S. & Shmookler Reis, R. J. Structural insights into pro-aggregation effects of C. elegans CRAM-1 and its human ortholog SERF2. Sci. Rep. (2018). Link
  • Zimmer, A. M. et al. Assessing the role of the acid-sensing ion channel ASIC4b in sodium uptake by larval zebrafish. Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 226, 1–10 (2018). Link
  • Zhang, Y. et al. Adult exposure to bisphenol A in rare minnow Gobiocypris rarus reduces sperm quality with disruption of testicular aquaporins. Chemosphere (2018). Link
  • Kolosov, D., Donini, A. & Kelly, S. P. Claudin-31 contributes to corticosteroid-induced alterations in the barrier properties of the gill epithelium. Mol. Cell. Endocrinol. (2017). Link
  • Xing, L. et al. Arabidopsis O‐GlcNAc transferase SEC activates histone methyltransferase ATX1 to regulate flowering. EMBO J. (2018). Link
  • Ma, Q. J. et al. An apple sucrose transporter MdSUT2.2 is a phosphorylation target for protein kinase MdCIPK22 in response to drought. Plant Biotechnol. J. (2019). Link
  • Jiang, P. et al. SIP1 participates in regulation of flowering time in rice by recruiting OsTrx1 to Ehd1. New Phytol. (2018). Link

Get in Touch
with GenScript Custom
Antibody Services

Get in Touch with GenScript Custom Antibody Services
Please enter a valid first name.
Please enter a valid last name.
Please enter a valid email name.
Please select a valid industry.
Please enter a valid institution/company.
Please select a valid country.
Please select a valid Zipcode.
Please enter a message in the textarea.

REQUEST A QUOTE