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Spink8 cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Spink8
Entrez Gene ID 78709
Full Name serine peptidase inhibitor, Kazal type 8
Synonyms C630041L24Rik
General protein information
Preferred Names

serine peptidase inhibitor, Kazal type 8

Names

serine protease inhibitor Kazal-type 8
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

9

9|9 F2

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006512398.3 XP_006512461.1 serine protease inhibitor Kazal-type 8 isoform X1
XM_006512398.4 XP_006512461.1 serine protease inhibitor Kazal-type 8 isoform X1
XM_006512398.2 XP_006512461.1 serine protease inhibitor Kazal-type 8 isoform X1
NM_183136.2 NP_898959.2 serine protease inhibitor Kazal-type 8 precursor

Pathways from BioSystems


Homologies


Canis lupus familiaris (dog) SPINK8 XP_005632653.1
Rattus norvegicus (Norway rat) Spink8 NP_001100330.1
LOC100429615 XP_002802856.1
Bos taurus (cattle) LOC101904056 XP_005223076.1
Homo sapiens (human) SPINK8 NP_001073994.1
Pan troglodytes (chimpanzee) SPINK8 XP_001145750.1
Mus musculus (house mouse) Spink8 NP_898959.2

Gene Ontology


Bibliography

Related articles in PubMed

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, Ikawa M, Matzuk MM
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)

Gene expression profile of the third pharyngeal pouch reveals role of mesenchymal MafB in embryonic thymus development.
Sultana DA, Tomita S, Hamada M, Iwanaga Y, Kitahama Y, Khang NV, Hirai S, Ohigashi I, Nitta S, Amagai T, Takahashi S, Takahama Y
Blood113(13)2976-87(2009 Mar)

Novel epididymal protease inhibitors with Kazal or WAP family domain.
Jalkanen J, Kotim?ki M, Huhtaniemi I, Poutanen M
Biochemical and biophysical research communications349(1)245-54(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spink8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spink8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu08380 NM_183136.2
Latest version!		 Mus musculus serine peptidase inhibitor, Kazal type 8 (Spink8), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OMu08380 XM_006512398.4
Latest version!		 Mus musculus serine peptidase inhibitor, Kazal type 8 (Spink8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
View Old Accession Versions >>
OMu08380 XM_006512398.3 Mus musculus serine peptidase inhibitor, Kazal type 8 (Spink8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
OMu08380 XM_006512398.2 Mus musculus serine peptidase inhibitor, Kazal type 8 (Spink8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu08380
Clone ID Related Accession (Same CDS sequence) XM_006512398.3 , XM_006512398.4 , XM_006512398.2 , NM_183136.2
Accession Version NM_183136.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 318bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-25
Organism Mus musculus(house mouse)
Product serine protease inhibitor Kazal-type 8 precursor
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC048588.1, CN843460.1 and AW455991.1. On Sep 7, 2006 this sequence version replaced NM_183136.1. ##Evidence-Data-START## Transcript exon combination :: CN836697.1, DV052899.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGAAGGTGA TCTTCTCAGT TGCAGTCCTT GTTCTGGCCA GCTCAGTGTG GACTTCCCTT 
GCAGTTGATT TCATCCTTCC TATGAACTTT CACATGACCG GGGAGCTTCT ACAAAAGACA 
AAGGCCTTGT GCATCAAGAA TATACAGTTA TGTTGGATAC TTAGCTACTT CAAGGTCAGT 
GAGCCTATAT GTGGCAGCAA CCAAGTGACC TACGAGGGCG AGTGCCATCT CTGCTCCGGA 
ATTCTATATG AAGACAGAAC TGTAATTAAA GTGCATGATG GGCCATGTGA ACACTCCTCG 
GATGAGTCTG AACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_898959.2
CDS136..453
Translation

Target ORF information:

RefSeq Version NM_183136.2
Organism Mus musculus(house mouse)
Definition Mus musculus serine peptidase inhibitor, Kazal type 8 (Spink8), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_183136.2

ORF Insert Sequence:

1
61
121
181
241
301
ATGAAGGTGA TCTTCTCAGT TGCAGTCCTT GTTCTGGCCA GCTCAGTGTG GACTTCCCTT 
GCAGTTGATT TCATCCTTCC TATGAACTTT CACATGACCG GGGAGCTTCT ACAAAAGACA 
AAGGCCTTGT GCATCAAGAA TATACAGTTA TGTTGGATAC TTAGCTACTT CAAGGTCAGT 
GAGCCTATAT GTGGCAGCAA CCAAGTGACC TACGAGGGCG AGTGCCATCT CTGCTCCGGA 
ATTCTATATG AAGACAGAAC TGTAATTAAA GTGCATGATG GGCCATGTGA ACACTCCTCG 
GATGAGTCTG AACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08380
Clone ID Related Accession (Same CDS sequence) XM_006512398.3 , XM_006512398.4 , XM_006512398.2 , NM_183136.2
Accession Version XM_006512398.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 318bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product serine protease inhibitor Kazal-type 8 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512398.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
ATGAAGGTGA TCTTCTCAGT TGCAGTCCTT GTTCTGGCCA GCTCAGTGTG GACTTCCCTT 
GCAGTTGATT TCATCCTTCC TATGAACTTT CACATGACCG GGGAGCTTCT ACAAAAGACA 
AAGGCCTTGT GCATCAAGAA TATACAGTTA TGTTGGATAC TTAGCTACTT CAAGGTCAGT 
GAGCCTATAT GTGGCAGCAA CCAAGTGACC TACGAGGGCG AGTGCCATCT CTGCTCCGGA 
ATTCTATATG AAGACAGAAC TGTAATTAAA GTGCATGATG GGCCATGTGA ACACTCCTCG 
GATGAGTCTG AACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006512461.1
CDS142..459
Translation

Target ORF information:

RefSeq Version XM_006512398.3
Organism Mus musculus(house mouse)
Definition Mus musculus serine peptidase inhibitor, Kazal type 8 (Spink8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006512398.3

ORF Insert Sequence:

1
61
121
181
241
301
ATGAAGGTGA TCTTCTCAGT TGCAGTCCTT GTTCTGGCCA GCTCAGTGTG GACTTCCCTT 
GCAGTTGATT TCATCCTTCC TATGAACTTT CACATGACCG GGGAGCTTCT ACAAAAGACA 
AAGGCCTTGT GCATCAAGAA TATACAGTTA TGTTGGATAC TTAGCTACTT CAAGGTCAGT 
GAGCCTATAT GTGGCAGCAA CCAAGTGACC TACGAGGGCG AGTGCCATCT CTGCTCCGGA 
ATTCTATATG AAGACAGAAC TGTAATTAAA GTGCATGATG GGCCATGTGA ACACTCCTCG 
GATGAGTCTG AACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08380
Clone ID Related Accession (Same CDS sequence) XM_006512398.3 , XM_006512398.4 , XM_006512398.2 , NM_183136.2
Accession Version XM_006512398.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 318bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2015-02-08
Organism Mus musculus(House mouse)
Product serine protease inhibitor Kazal-type 8 isoform X1
Comment 

1
61
121
181
241
301
ATGAAGGTGA TCTTCTCAGT TGCAGTCCTT GTTCTGGCCA GCTCAGTGTG GACTTCCCTT 
GCAGTTGATT TCATCCTTCC TATGAACTTT CACATGACCG GGGAGCTTCT ACAAAAGACA 
AAGGCCTTGT GCATCAAGAA TATACAGTTA TGTTGGATAC TTAGCTACTT CAAGGTCAGT 
GAGCCTATAT GTGGCAGCAA CCAAGTGACC TACGAGGGCG AGTGCCATCT CTGCTCCGGA 
ATTCTATATG AAGACAGAAC TGTAATTAAA GTGCATGATG GGCCATGTGA ACACTCCTCG 
GATGAGTCTG AACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006512461.1
Translation

Target ORF information:

RefSeq Version XM_006512398.2
Organism Mus musculus(House mouse)
Definition Mus musculus serine peptidase inhibitor, Kazal type 8 (Spink8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006512398.2

ORF Insert Sequence:

1
61
121
181
241
301
ATGAAGGTGA TCTTCTCAGT TGCAGTCCTT GTTCTGGCCA GCTCAGTGTG GACTTCCCTT 
GCAGTTGATT TCATCCTTCC TATGAACTTT CACATGACCG GGGAGCTTCT ACAAAAGACA 
AAGGCCTTGT GCATCAAGAA TATACAGTTA TGTTGGATAC TTAGCTACTT CAAGGTCAGT 
GAGCCTATAT GTGGCAGCAA CCAAGTGACC TACGAGGGCG AGTGCCATCT CTGCTCCGGA 
ATTCTATATG AAGACAGAAC TGTAATTAAA GTGCATGATG GGCCATGTGA ACACTCCTCG 
GATGAGTCTG AACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu08380
Clone ID Related Accession (Same CDS sequence) XM_006512398.3 , XM_006512398.4 , XM_006512398.2 , NM_183136.2
Accession Version XM_006512398.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 318bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product serine protease inhibitor Kazal-type 8 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512398.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
ATGAAGGTGA TCTTCTCAGT TGCAGTCCTT GTTCTGGCCA GCTCAGTGTG GACTTCCCTT 
GCAGTTGATT TCATCCTTCC TATGAACTTT CACATGACCG GGGAGCTTCT ACAAAAGACA 
AAGGCCTTGT GCATCAAGAA TATACAGTTA TGTTGGATAC TTAGCTACTT CAAGGTCAGT 
GAGCCTATAT GTGGCAGCAA CCAAGTGACC TACGAGGGCG AGTGCCATCT CTGCTCCGGA 
ATTCTATATG AAGACAGAAC TGTAATTAAA GTGCATGATG GGCCATGTGA ACACTCCTCG 
GATGAGTCTG AACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006512461.1
CDS141..458
Translation

Target ORF information:

RefSeq Version XM_006512398.4
Organism Mus musculus(house mouse)
Definition Mus musculus serine peptidase inhibitor, Kazal type 8 (Spink8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006512398.4

ORF Insert Sequence:

1
61
121
181
241
301
ATGAAGGTGA TCTTCTCAGT TGCAGTCCTT GTTCTGGCCA GCTCAGTGTG GACTTCCCTT 
GCAGTTGATT TCATCCTTCC TATGAACTTT CACATGACCG GGGAGCTTCT ACAAAAGACA 
AAGGCCTTGT GCATCAAGAA TATACAGTTA TGTTGGATAC TTAGCTACTT CAAGGTCAGT 
GAGCCTATAT GTGGCAGCAA CCAAGTGACC TACGAGGGCG AGTGCCATCT CTGCTCCGGA 
ATTCTATATG AAGACAGAAC TGTAATTAAA GTGCATGATG GGCCATGTGA ACACTCCTCG 
GATGAGTCTG AACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice.
Proceedings of the National Academy of Sciences of the United States of America113(28)7704-10(2016 07)
Miyata H,Castaneda JM,Fujihara Y,Yu Z,Archambeault DR,Isotani A,Kiyozumi D,Kriseman ML,Mashiko D,Matsumura T,Matzuk RM,Mori M,Noda T,Oji A,Okabe M,Prunskaite-Hyyrylainen R,Ramirez-Solis R,Satouh Y,Zhang Q,Ikawa M,Matzuk MM


book

Gene expression profile of the third pharyngeal pouch reveals role of mesenchymal MafB in embryonic thymus development.
Blood113(13)2976-87(2009 Mar)
Sultana DA,Tomita S,Hamada M,Iwanaga Y,Kitahama Y,Khang NV,Hirai S,Ohigashi I,Nitta S,Amagai T,Takahashi S,Takahama Y


book

Novel epididymal protease inhibitors with Kazal or WAP family domain.
Biochemical and biophysical research communications349(1)245-54(2006 Oct)
Jalkanen J,Kotim?ki M,Huhtaniemi I,Poutanen M