NTHK1 cDNA ORF clone, Nicotiana tabacum(common tobacco)

Gene
Clones
gRNAs

Summary

Gene Symbol	NTHK1
Entrez Gene ID	107825743
Full Name	histidine kinase1
Gene Type	protein-coding
Organism	Nicotiana tabacum(common tobacco)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001326157.1	NP_001313086.1	histidine kinase1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Tobacco Translationally Controlled Tumor Protein Interacts with Ethylene Receptor Tobacco Histidine Kinase1 and Enhances Plant Growth through Promotion of Cell Proliferation.
Tao JJ, Cao YR, Chen HW, Wei W, Li QT, Ma B, Zhang WK, Chen SY, Zhang JS
Plant physiology169(1)96-114(2015 Sep)

Serine/threonine kinase activity in the putative histidine kinase-like ethylene receptor NTHK1 from tobacco.
Xie C, Zhang JS, Zhou HL, Li J, Zhang ZG, Wang DW, Chen SY
The Plant journal : for cell and molecular biology33(2)385-93(2003 Jan)

Spatial expression and characterization of a putative ethylene receptor protein NTHK1 in tobacco.
Xie C, Zhang ZG, Zhang JS, He XJ, Cao WH, He SJ, Chen SY
Plant & cell physiology43(7)810-5(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Growth inhibition triggered by ethylene is via feedback regulation, in which ethylene-induced NtTCTP associates with and stabilizes NTHK1 to reduce response to ethylene and promote growth through acceleration of cell proliferation. [NTHK1]
Title: Tobacco Translationally Controlled Tumor Protein Interacts with Ethylene Receptor Tobacco Histidine Kinase1 and Enhances Plant Growth through Promotion of Cell Proliferation.

The following NTHK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NTHK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONi69245	NM_001326157.1 Latest version!	Nicotiana tabacum histidine kinase1 (NTHK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NTHK1 ( NM_001326157.1 ) cDNA ORF clone, Nicotiana tabacum(common tobacco) -> NP_001313086.1 Nicotiana tabacum histidine kinase1 (NTHK1), mRNA.

Price & Availability↑

CloneID	ONi69245
Clone ID Related Accession (Same CDS sequence)	NM_001326157.1
Accession Version	NM_001326157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-25
Organism	Nicotiana tabacum(common tobacco)
Product	histidine kinase1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF026267.3. On May 13, 2016 this sequence version replaced XM_016652648.1. ##Evidence-Data-START## Transcript exon combination :: AF026267.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3338465, SAMEA3338466 [ECO:0000348] ##Evidence-Data-END##

ATGTTAAGGA CATTAGCATT AGCTTTGTTA ATTTCGGCAT TCCTTGTTTC CTTATCCGCT 
GCTGATAATG GTTTCCCTCG TTGTAACTGT GATGATGAAG GATTTTGGAG CATTGAGCGT 
ATCTTAGAGT GCCAAAGAAT CAGTGATTTA TTTATTGCGA TAGCCTATTT TTCCATCCCA 
ATTGAGCTCC TTTACTTCGT AAGCTGTTCC AACTTTCCAT TCAAATGGGT GCTCTTCCAA 
TTTATTGCAT TCATTGTTCT GTGTGGGATG ACTCATTTGC TCAATTTCTG GACTTACTAT 
GGCCAACACC CATTTCAGCT TATGCTTGCT CTAACCATTT TTAAAGTCCT CACTGCACTA 
GTATCCTTTG CCACTGCTAT AACCCTTATC ACCCTCTTTC CTATGCTGCT CAAAATCAAA 
GTGAGGGAGT TTATGCTGAA AAAGAAAACT TGGGATCTTG GTAGAGAAGT TGGATTAATA 
AAGCAGCAAA AGGAAGCTGG ATGGCATGTC CGGATGCTTA CACAGGAGAT TCGAAAGTCA 
CTTGATCGTC ATACAATACT ATACACTACT CTGGTGGAGT TAGCAAAGAC ACTGGATTTG 
CATAATTGTG CTATTTGGAA GCCCAATGAG AATAAAACTG AGATGAACCT AATTCATGAG 
CTGAAAGGAA GAAGCTTTTC TAGTATGTAT AATTTACCTA TCCCGACAAG TGATCCAGAT 
GTAAGGGAGA TTAAGGAGAG TGATGGAGTA AAGTTACTTG ATGCCTACTC CCCACTTGCT 
GCTGCGAGTA GTGGAGGGAG TAGTGAACCA GGAGCTGTGG CTGCAATTAG GATGCCAATG 
CTGAAGGTGT CGAACTTCAA AGGTGGAACT CCTGAACTTG TCCCAGAATG TTATGCCATA 
CTGGTGTTGG TTCTTCCTGG TGAACAAGGT AGATCTTGGA GCAACCAGGA AATTGAGATA 
GTCAGGGTAG TGGCTGATCA GGTTGCTGTG GCTCTGTCCC ATGCTGCAGT TCTTGAAGAG 
TCTCAGCATA TGAGAGAAAC ATTGGAGGAG CAAAATCGAG CTCTGCAACA AGCGAAGCAG 
GATGCACTTA GGGCGAGTCA AGCTAGGAAT GCATTTCAGA TGGTTATGAG CCATGGGTTG 
AGAAGACCCA TGCACTCAAT TTTGGGTCTT CTCTCCTTGT TGCAAGATGA TAACATGGGT 
ATTGAGCAGC GGCTTCTTGT AGATGCAATG GCTAAAACCA GCAGTGTTGT ATCAACCCTA 
ATAAATGATG TGATGGATAC TTCGACAAAG GACAATAGTA GATTTCCTTT GGAGATGAGG 
CATTTTCAGC TACATTCCAT GATAAAAGAA GCTGCTTGTC TTGCCAAGTG TTTGTGTGCT 
CATAGGGGTT ACAATATTTC CATCGAGGTT GACAAATCTT TGCCAAATTA TGTCATGGGT 
GATGAAAGAA GAGTTTTTCA AGTTATTCTT CATATGGTTG GGAATCTTTT GAAGGACCCC 
AATGGAGGTT GTCTTACATT TAGAGTTCTC CCAGAAAGTG CAAGAAGGGA AGGCATCGAC 
GGAGCTTGGA AGACAAGGAG ATCACACTCA TCTCTTGAAA ATGTCTATAT CAGGTTTGAA 
GTTGGGTCAA GCAATAATCA TTCTCAGCCA GAGGGCATAA CATCCACATT GCCACATTGT 
TGTGAAACAC GCCGCAGTAG GGAAGTGGAG GAAAGGTTGA GCTTTTCTGT GTGCAGAAAG 
CTGGTTCAGT TGATGCAAGG AGACATCTGG GTAGTCCCAA ATCCAGAAGG TTTTGATCAA 
AGCATGACTG TCATTCTTGG GTTTCAACTG CGGCCATCAA TTGCCGTAGG CATTCCTGAA 
TATGGAGAAT CTTCTGATCA CTCGCATCCA CACTCACTCC TCCAGGGGGT TAATGTTCTC 
TTAGCAGATT ATGATGATGT GAATAGAGCT GTAACAAGGA AGCTACTTGA AAAATTAGGA 
TGCACTGTTT CTGCAGTTTC ATCGGGACAC GACTGTCTTG GTGCTCTTGG TCCTGCTGTA 
TCTTCATTCC AAATTGTCCT TTTGGATCTT CACCTGCCTG ATTTAGACGG CTTCGAAGTA 
ACCATGAGAA TTCGGGAGTT TCGTAGCCGC AACTGGCCAT TGATCGTTGG TTTTGCATCC 
GCTGATGAAG ATGTTAGTGG AAGATGCCTG CAGATTGGAA TGAATGGAAT TATTCGTAAG 
CCGGTGCTAC TGCCTGGAAT TGCTGATGAG CTTCAAAGGG TCCTTCTACA GGCAAGCATA 
ATCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001313086.1
CDS	247..2535
Translation	MLRTLALALLISAFLVSLSAADNGFPRCNCDDEGFWSIERILECQRISDLFIAIAYFSIPIELLYFVSCSNFPFKWVLFQFIAFIVLCGMTHLLNFWTYYGQHPFQLMLALTIFKVLTALVSFATAITLITLFPMLLKIKVREFMLKKKTWDLGREVGLIKQQKEAGWHVRMLTQEIRKSLDRHTILYTTLVELAKTLDLHNCAIWKPNENKTEMNLIHELKGRSFSSMYNLPIPTSDPDVREIKESDGVKLLDAYSPLAAASSGGSSEPGAVAAIRMPMLKVSNFKGGTPELVPECYAILVLVLPGEQGRSWSNQEIEIVRVVADQVAVALSHAAVLEESQHMRETLEEQNRALQQAKQDALRASQARNAFQMVMSHGLRRPMHSILGLLSLLQDDNMGIEQRLLVDAMAKTSSVVSTLINDVMDTSTKDNSRFPLEMRHFQLHSMIKEAACLAKCLCAHRGYNISIEVDKSLPNYVMGDERRVFQVILHMVGNLLKDPNGGCLTFRVLPESARREGIDGAWKTRRSHSSLENVYIRFEVGSSNNHSQPEGITSTLPHCCETRRSREVEERLSFSVCRKLVQLMQGDIWVVPNPEGFDQSMTVILGFQLRPSIAVGIPEYGESSDHSHPHSLLQGVNVLLADYDDVNRAVTRKLLEKLGCTVSAVSSGHDCLGALGPAVSSFQIVLLDLHLPDLDGFEVTMRIREFRSRNWPLIVGFASADEDVSGRCLQIGMNGIIRKPVLLPGIADELQRVLLQASIIT

Target ORF information:

RefSeq Version	NM_001326157.1
Organism	Nicotiana tabacum(common tobacco)
Definition	Nicotiana tabacum histidine kinase1 (NTHK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001326157.1

ORF Insert Sequence:

ATGTTAAGGA CATTAGCATT AGCTTTGTTA ATTTCGGCAT TCCTTGTTTC CTTATCCGCT 
GCTGATAATG GTTTCCCTCG TTGTAACTGT GATGATGAAG GATTTTGGAG CATTGAGCGT 
ATCTTAGAGT GCCAAAGAAT CAGTGATTTA TTTATTGCGA TAGCCTATTT TTCCATCCCA 
ATTGAGCTCC TTTACTTCGT AAGCTGTTCC AACTTTCCAT TCAAATGGGT GCTCTTCCAA 
TTTATTGCAT TCATTGTTCT GTGTGGGATG ACTCATTTGC TCAATTTCTG GACTTACTAT 
GGCCAACACC CATTTCAGCT TATGCTTGCT CTAACCATTT TTAAAGTCCT CACTGCACTA 
GTATCCTTTG CCACTGCTAT AACCCTTATC ACCCTCTTTC CTATGCTGCT CAAAATCAAA 
GTGAGGGAGT TTATGCTGAA AAAGAAAACT TGGGATCTTG GTAGAGAAGT TGGATTAATA 
AAGCAGCAAA AGGAAGCTGG ATGGCATGTC CGGATGCTTA CACAGGAGAT TCGAAAGTCA 
CTTGATCGTC ATACAATACT ATACACTACT CTGGTGGAGT TAGCAAAGAC ACTGGATTTG 
CATAATTGTG CTATTTGGAA GCCCAATGAG AATAAAACTG AGATGAACCT AATTCATGAG 
CTGAAAGGAA GAAGCTTTTC TAGTATGTAT AATTTACCTA TCCCGACAAG TGATCCAGAT 
GTAAGGGAGA TTAAGGAGAG TGATGGAGTA AAGTTACTTG ATGCCTACTC CCCACTTGCT 
GCTGCGAGTA GTGGAGGGAG TAGTGAACCA GGAGCTGTGG CTGCAATTAG GATGCCAATG 
CTGAAGGTGT CGAACTTCAA AGGTGGAACT CCTGAACTTG TCCCAGAATG TTATGCCATA 
CTGGTGTTGG TTCTTCCTGG TGAACAAGGT AGATCTTGGA GCAACCAGGA AATTGAGATA 
GTCAGGGTAG TGGCTGATCA GGTTGCTGTG GCTCTGTCCC ATGCTGCAGT TCTTGAAGAG 
TCTCAGCATA TGAGAGAAAC ATTGGAGGAG CAAAATCGAG CTCTGCAACA AGCGAAGCAG 
GATGCACTTA GGGCGAGTCA AGCTAGGAAT GCATTTCAGA TGGTTATGAG CCATGGGTTG 
AGAAGACCCA TGCACTCAAT TTTGGGTCTT CTCTCCTTGT TGCAAGATGA TAACATGGGT 
ATTGAGCAGC GGCTTCTTGT AGATGCAATG GCTAAAACCA GCAGTGTTGT ATCAACCCTA 
ATAAATGATG TGATGGATAC TTCGACAAAG GACAATAGTA GATTTCCTTT GGAGATGAGG 
CATTTTCAGC TACATTCCAT GATAAAAGAA GCTGCTTGTC TTGCCAAGTG TTTGTGTGCT 
CATAGGGGTT ACAATATTTC CATCGAGGTT GACAAATCTT TGCCAAATTA TGTCATGGGT 
GATGAAAGAA GAGTTTTTCA AGTTATTCTT CATATGGTTG GGAATCTTTT GAAGGACCCC 
AATGGAGGTT GTCTTACATT TAGAGTTCTC CCAGAAAGTG CAAGAAGGGA AGGCATCGAC 
GGAGCTTGGA AGACAAGGAG ATCACACTCA TCTCTTGAAA ATGTCTATAT CAGGTTTGAA 
GTTGGGTCAA GCAATAATCA TTCTCAGCCA GAGGGCATAA CATCCACATT GCCACATTGT 
TGTGAAACAC GCCGCAGTAG GGAAGTGGAG GAAAGGTTGA GCTTTTCTGT GTGCAGAAAG 
CTGGTTCAGT TGATGCAAGG AGACATCTGG GTAGTCCCAA ATCCAGAAGG TTTTGATCAA 
AGCATGACTG TCATTCTTGG GTTTCAACTG CGGCCATCAA TTGCCGTAGG CATTCCTGAA 
TATGGAGAAT CTTCTGATCA CTCGCATCCA CACTCACTCC TCCAGGGGGT TAATGTTCTC 
TTAGCAGATT ATGATGATGT GAATAGAGCT GTAACAAGGA AGCTACTTGA AAAATTAGGA 
TGCACTGTTT CTGCAGTTTC ATCGGGACAC GACTGTCTTG GTGCTCTTGG TCCTGCTGTA 
TCTTCATTCC AAATTGTCCT TTTGGATCTT CACCTGCCTG ATTTAGACGG CTTCGAAGTA 
ACCATGAGAA TTCGGGAGTT TCGTAGCCGC AACTGGCCAT TGATCGTTGG TTTTGCATCC 
GCTGATGAAG ATGTTAGTGG AAGATGCCTG CAGATTGGAA TGAATGGAAT TATTCGTAAG 
CCGGTGCTAC TGCCTGGAAT TGCTGATGAG CTTCAAAGGG TCCTTCTACA GGCAAGCATA 
ATCACGTGA