HEATR5B cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEATR5B
Entrez Gene ID	100076343
Full Name	HEAT repeat containing 5B
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029050042.1	XP_028905875.1	HEAT repeat-containing protein 5B isoform X1
XM_003428570.3	XP_003428618.1	HEAT repeat-containing protein 5B
XM_029050045.1	XP_028905878.1	HEAT repeat-containing protein 5B isoform X3
XM_029050043.1	XP_028905876.1	HEAT repeat-containing protein 5B isoform X1
XM_029050044.1	XP_028905877.1	HEAT repeat-containing protein 5B isoform X2

Pathways from BioSystems
Gene Ontology

The following HEATR5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEATR5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc02765	XM_003428570.3 Latest version!	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OOc135026	XM_029050042.1 Latest version!	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc135026	XM_029050043.1 Latest version!	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc135027	XM_029050044.1 Latest version!	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc135028	XM_029050045.1 Latest version!	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEATR5B ( XM_003428570.3 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_003428618.1 Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), mRNA.

Price & Availability↑

CloneID	OOc02765
Clone ID Related Accession (Same CDS sequence)	XM_003428570.3
Accession Version	XM_003428570.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-14
Organism	Ornithorhynchus anatinus(platypus)
Product	HEAT repeat-containing protein 5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009114.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 15, 2016 this sequence version replaced XM_003428570.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT CGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAACAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
AATATCGATC CTTTTGCAGC AGGAGTAGCC TCTCGGAGGG ATCTCCACTG CCGGCACCAG 
GGCGTCAACA TAACGGAGAC CGGCCTCGAA GGACTGCTTT TTGGGATGCT GGACCGCGAG 
ACGGATCGAA AACTCTGCTC CGACATCCAC GATACCCTGG GACATATGCT TTCGTCTCTG 
GCAGTCGAAA AGCTCTCGCA TTGGTTGATG CTGTGTAAAG ATGTTCTCGC CGCTTCCGGT 
GACATGAGCG CCGCGAATCC TTTCGGGGGA CTGAAGGATG AGGAATCCGA AAAAAAGGAC 
GAGATGGATG ACGATACCAT GTTCACCACC CTGGGAGAAG ACGAGAAGTC CAAACCTTCT 
GTAGCTCCTC GCTGGGCCAC TCGTGTATTT GCCGCTGACT GCCTGTGTCG AATCATCTTG 
CTCTGCGAGA ACGCTGACCA GGCCCATTTC GATCTCGCCC TAGCCCGTTC GGCCAAACTT 
CGGAACCCAA AAAATGACCT CTTAGTGCTC CACCTCTCCG ACCTGATCCG TATGGCATTT 
ATGGCTGCCA CGGATCATAG CAACCAGCTG CGGATGGCTG GACTCCAAAC CCTCGAGGAC 
ATTATCAAGA AGTTTGCATC TGTGCCCGAG CCAGAATTTC CAGGCCACGT AATATTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTTTA AGACCAGCTT TTTCACAAGA TACTCCATCA 
GATATAATAG CAAAAGCTTG CCAGGTCTGC AGTACCTGGA TTGGCAGCGG AGTCGTAAGC 
GACCTCAATG ACCTTCGCCG AGTCCACAAT TTGCTGGTCT CCTCCCTGGA CAAAGTACAG 
GCGGGAAAAG GGTCTTCAAG CCAACTTTAT AGAGAGAGTG CCACCACCAT GGAAAAACTG 
GCTGTCCTGA AAGCTTGGGC TGAGGTCTAC GTCGTTGCGA TGAAAATTAA GAGGGAAGCC 
GAGATAAAGC CAAAAAGAGC CGTTAAGAGC ACGGAGGAAG ACGACGACGA TTATGGGGCT 
GCAGATGAGC TGCCTCCAGA CAGCTTAATA ACCCTGGTGC AGCCCGAGCT GCCTATACTT 
AGCCGCCTTT GGTTAGCGGC GTTAAAAGAC TACGCGCTTC TGACTCTGCC CGCTGAATTC 
GCCAGCCAGC TCCCGCCAGA TGGCGGGGCC TTTTATACTC CCGAGACCAT CGATACCGCT 
AGGCTTCACT ATCGGAATTC TTGGGCTCCA ATTCTCCACG CAGCGGCCCT CTGGCTGAAC 
AGCACGGGAT TCGACACCCC GGAGGTCGCG GAAGAAGCGG CGGCGGCCGC CGTCTCCGGT 
TCTCAGAAGC GTCCTACGTC CGTCCCGTTA AACCAGGCGC CTGGGCCAAC TCCCGATACG 
AAATCTTTAC CCGAAGTAAA CAGAGCCCGA ATGCATCTGA TTTTAGGTGT CAGTATACAG 
TTCCTCTGCT CCCCAAGACC CGAAGAACCT ATTGAACACG TTACAGCCTG TCTTCAAGCA 
CTCCGTACCT TACTGGACTC TCCCTGTGCC AGAATCCATA TTGCAGAAGA TCAGCTCGTT 
GGCGTGGAGT TGCTGAGTGT GGTACATCGA CTCCTTTTGA CCTGGAATCC GCCGTCGGTC 
CAGTTGCTGG TTACCGGAGT AGTGCAACAG ATCGTAAGGG CGGCTCAGGA CTATTTGCAG 
GAAAAAAGGA ACACGCTAAG CTGTATGACG ATACTCCCCA CGATCCTGTT TCTCATTGCG 
AGAATCTTGA AGGACACGGC AATTAAGTCG GCGGATAATC AGGTCCCCCC TCCGGTCAGC 
GCGGCTTTAC AGGGGATTAA AAATATCGTG ACTCTCCCAC TGGTGAAGAC CGACGAAACG 
AGCAAGCAGT GGACCGAACT GATTCGCAGC ACTCTCGCTT GTATCCTGGA ATATTCTCAA 
CCAGATGAGT CTTTGCCCAC TCTTGACGAA GTGAGCATGC TCACAGCTAT CGCACTCTTC 
CTGTGGTCCG CAAGTACTGA AATAATAGGA GTCCAGTCGT TACAGAACGG CTGCATGAAC 
AGATTTAAGA ACGCGTTGAA TTCCTGCGAT CCGTGGGTTC AGGCCAAATG TTACCAACTT 
CTCCTTTCAC TGTTCCAACA CTCCAATCGG GCCCTCTCAA CTCCGTACAT CCATTCATTG 
GCCCCGATAA TGGTTGAAAA ACTGAAAGCC GTGGAGAGGA ACCGGCCAAA CAGCAACATA 
GAACTTTTAG CGGTCCAAGA AGGGATCAAA GTCCTCGAAA CCTTGGTTGC CCTTGGTGAA 
GAGCAGAACA GAGTGCAGCT GCTTGCCCTC TTGGTTCCCA CTTTGATCTC TTATCTACTG 
GATGAAAACG CTTTTGCTTC GGCACCTCTG GCCTCCAGAG ATCTGCACGA GTTTGCCCTG 
CAGAATCTGA TGCGTATCGG ACCCCTCTAC CCTCACGCTT TCAAGACGGT AATGGGAGCC 
GCTCCCGAGT TGAAGATCCG TCTGGAAACT GCAGTGAGAG CGAGCCAGGC CAGCAAAGCC 
AAGGCAGCCG CCCGGCAGCC GGCCCCCACC GCACACTCTG CCCCGACGAT CAAACTCAAG 
ACGAGCTTTT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003428618.1
CDS	63..6017
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAGYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLKKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENVATLCFKALESSNYGVRVAVSKLLGTVMATALMPKQAAVMRQNVKRATLEEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERNFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEIVVNDTSGENKSGAAEIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHIEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESGGSNIDPFAAGVASRRDLHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASGDMSAANPFGGLKDEESEKKDEMDDDTMFTTLGEDEKSKPSVAPRWATRVFAADCLCRIILLCENADQAHFDLALARSAKLRNPKNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQTLEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMKIKREAEIKPKRAVKSTEEDDDDYGAADELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAAALWLNSTGFDTPEVAEEAAAAAVSGSQKRPTSVPLNQAPGPTPDTKSLPEVNRARMHLILGVSIQFLCSPRPEEPIEHVTACLQALRTLLDSPCARIHIAEDQLVGVELLSVVHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLSCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKNIVTLPLVKTDETSKQWTELIRSTLACILEYSQPDESLPTLDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWVQAKCYQLLLSLFQHSNRALSTPYIHSLAPIMVEKLKAVERNRPNSNIELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENAFASAPLASRDLHEFALQNLMRIGPLYPHAFKTVMGAAPELKIRLETAVRASQASKAKAAARQPAPTAHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_003428570.3
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003428570.3

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT CGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAACAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
AATATCGATC CTTTTGCAGC AGGAGTAGCC TCTCGGAGGG ATCTCCACTG CCGGCACCAG 
GGCGTCAACA TAACGGAGAC CGGCCTCGAA GGACTGCTTT TTGGGATGCT GGACCGCGAG 
ACGGATCGAA AACTCTGCTC CGACATCCAC GATACCCTGG GACATATGCT TTCGTCTCTG 
GCAGTCGAAA AGCTCTCGCA TTGGTTGATG CTGTGTAAAG ATGTTCTCGC CGCTTCCGGT 
GACATGAGCG CCGCGAATCC TTTCGGGGGA CTGAAGGATG AGGAATCCGA AAAAAAGGAC 
GAGATGGATG ACGATACCAT GTTCACCACC CTGGGAGAAG ACGAGAAGTC CAAACCTTCT 
GTAGCTCCTC GCTGGGCCAC TCGTGTATTT GCCGCTGACT GCCTGTGTCG AATCATCTTG 
CTCTGCGAGA ACGCTGACCA GGCCCATTTC GATCTCGCCC TAGCCCGTTC GGCCAAACTT 
CGGAACCCAA AAAATGACCT CTTAGTGCTC CACCTCTCCG ACCTGATCCG TATGGCATTT 
ATGGCTGCCA CGGATCATAG CAACCAGCTG CGGATGGCTG GACTCCAAAC CCTCGAGGAC 
ATTATCAAGA AGTTTGCATC TGTGCCCGAG CCAGAATTTC CAGGCCACGT AATATTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTTTA AGACCAGCTT TTTCACAAGA TACTCCATCA 
GATATAATAG CAAAAGCTTG CCAGGTCTGC AGTACCTGGA TTGGCAGCGG AGTCGTAAGC 
GACCTCAATG ACCTTCGCCG AGTCCACAAT TTGCTGGTCT CCTCCCTGGA CAAAGTACAG 
GCGGGAAAAG GGTCTTCAAG CCAACTTTAT AGAGAGAGTG CCACCACCAT GGAAAAACTG 
GCTGTCCTGA AAGCTTGGGC TGAGGTCTAC GTCGTTGCGA TGAAAATTAA GAGGGAAGCC 
GAGATAAAGC CAAAAAGAGC CGTTAAGAGC ACGGAGGAAG ACGACGACGA TTATGGGGCT 
GCAGATGAGC TGCCTCCAGA CAGCTTAATA ACCCTGGTGC AGCCCGAGCT GCCTATACTT 
AGCCGCCTTT GGTTAGCGGC GTTAAAAGAC TACGCGCTTC TGACTCTGCC CGCTGAATTC 
GCCAGCCAGC TCCCGCCAGA TGGCGGGGCC TTTTATACTC CCGAGACCAT CGATACCGCT 
AGGCTTCACT ATCGGAATTC TTGGGCTCCA ATTCTCCACG CAGCGGCCCT CTGGCTGAAC 
AGCACGGGAT TCGACACCCC GGAGGTCGCG GAAGAAGCGG CGGCGGCCGC CGTCTCCGGT 
TCTCAGAAGC GTCCTACGTC CGTCCCGTTA AACCAGGCGC CTGGGCCAAC TCCCGATACG 
AAATCTTTAC CCGAAGTAAA CAGAGCCCGA ATGCATCTGA TTTTAGGTGT CAGTATACAG 
TTCCTCTGCT CCCCAAGACC CGAAGAACCT ATTGAACACG TTACAGCCTG TCTTCAAGCA 
CTCCGTACCT TACTGGACTC TCCCTGTGCC AGAATCCATA TTGCAGAAGA TCAGCTCGTT 
GGCGTGGAGT TGCTGAGTGT GGTACATCGA CTCCTTTTGA CCTGGAATCC GCCGTCGGTC 
CAGTTGCTGG TTACCGGAGT AGTGCAACAG ATCGTAAGGG CGGCTCAGGA CTATTTGCAG 
GAAAAAAGGA ACACGCTAAG CTGTATGACG ATACTCCCCA CGATCCTGTT TCTCATTGCG 
AGAATCTTGA AGGACACGGC AATTAAGTCG GCGGATAATC AGGTCCCCCC TCCGGTCAGC 
GCGGCTTTAC AGGGGATTAA AAATATCGTG ACTCTCCCAC TGGTGAAGAC CGACGAAACG 
AGCAAGCAGT GGACCGAACT GATTCGCAGC ACTCTCGCTT GTATCCTGGA ATATTCTCAA 
CCAGATGAGT CTTTGCCCAC TCTTGACGAA GTGAGCATGC TCACAGCTAT CGCACTCTTC 
CTGTGGTCCG CAAGTACTGA AATAATAGGA GTCCAGTCGT TACAGAACGG CTGCATGAAC 
AGATTTAAGA ACGCGTTGAA TTCCTGCGAT CCGTGGGTTC AGGCCAAATG TTACCAACTT 
CTCCTTTCAC TGTTCCAACA CTCCAATCGG GCCCTCTCAA CTCCGTACAT CCATTCATTG 
GCCCCGATAA TGGTTGAAAA ACTGAAAGCC GTGGAGAGGA ACCGGCCAAA CAGCAACATA 
GAACTTTTAG CGGTCCAAGA AGGGATCAAA GTCCTCGAAA CCTTGGTTGC CCTTGGTGAA 
GAGCAGAACA GAGTGCAGCT GCTTGCCCTC TTGGTTCCCA CTTTGATCTC TTATCTACTG 
GATGAAAACG CTTTTGCTTC GGCACCTCTG GCCTCCAGAG ATCTGCACGA GTTTGCCCTG 
CAGAATCTGA TGCGTATCGG ACCCCTCTAC CCTCACGCTT TCAAGACGGT AATGGGAGCC 
GCTCCCGAGT TGAAGATCCG TCTGGAAACT GCAGTGAGAG CGAGCCAGGC CAGCAAAGCC 
AAGGCAGCCG CCCGGCAGCC GGCCCCCACC GCACACTCTG CCCCGACGAT CAAACTCAAG 
ACGAGCTTTT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_029050042.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028905875.1 Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

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CloneID	OOc135026
Clone ID Related Accession (Same CDS sequence)	XM_029050042.1 , XM_029050043.1
Accession Version	XM_029050042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT AGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAGCAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
AATATCGATC CTTTTGCAGC AGGAGTAGCC TCTCGGAGGG ATCTCCACTG CCGGCACCAG 
GGCGTCAACA TAACGGAGAC CGGCCTCGAA GGACTGCTTT TTGGGATGCT GGACCGCGAG 
ACGGATCGAA AACTCTGCTC CGACATCCAC GATACCCTGG GACATATGCT TTCGTCTCTG 
GCAGTCGAAA AGCTCTCGCA TTGGTTGATG CTGTGTAAAG ATGTTCTCGC CGCTTCCGGT 
GACATGAGCG CCGCGAATCC TTTCGGGGGA CTGAAGGATG AGGAATCCGA AAAAAAGGAC 
GAGATGGATG ACGATACCAT GTTCACCACC CTGGGAGAAG ACGAGAAGTC CAAACCTTCT 
GTAGCTCCTC GCTGGGCCAC TCGTGTATTT GCCGCTGACT GCCTGTGTCG AATCATCTTG 
CTCTGCGAGA ACGCTGACCA GGCCCATTTC GATCTCGCCC TAGCCCGTTC GGCCAAACTT 
CGGAACCCAA AAAATGACCT CTTAGTGCTC CACCTCTCCG ACCTGATCCG TATGGCATTT 
ATGGCTGCCA CGGATCATAG CAACCAGCTG CGGATGGCTG GACTCCAAAC CCTCGAGGAC 
ATTATCAAGA AGTTTGCATC TGTGCCCGAG CCAGAATTTC CAGGCCACGT AATATTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTTTA AGACCAGCTT TTTCACAAGA TACTCCATCA 
GATATAATAG CAAAAGCTTG CCAGGTCTGC AGTACCTGGA TTGGCAGCGG AGTCGTAAGC 
GACCTCAATG ACCTTCGCCG AGTCCACAAT TTGCTGGTCT CCTCCCTGGA CAAAGTACAG 
GCGGGAAAAG GGTCTTCAAG CCAACTTTAT AGAGAGAGTG CCACCACCAT GGAAAAACTG 
GCTGTCCTGA AAGCTTGGGC TGAGGTCTAC GTCGTTGCGA TGAAAATTAA GAGGGAAGCC 
GAGATAAAGC CAAAAAGAGC CGTTAAGAGC ACGGAGGAAG ACGACGACGA TTATGGGGCT 
GCAGATGAGC TGCCTCCAGA CAGCTTAATA ACCCTGGTGC AGCCCGAGCT GCCTATACTT 
AGCCGCCTTT GGTTAGCGGC GTTAAAAGAC TACGCGCTTC TGACTCTGCC CGCTGAATTC 
GCCAGCCAGC TCCCGCCAGA TGGCGGGGCC TTTTATACTC CCGAGACCAT CGATACCGCT 
AGGCTTCACT ATCGGAATTC TTGGGCTCCA ATTCTCCACG CAGCGGCCCT CTGGCTGAAC 
AGCACGGGAT TCGACACCCC GGAGGTCGCG GAAGAAGCGG CGGCGGCCGC CGTCTCCGGT 
TCTCAGAAGC GTCCTACGTC CGTCCCGTTA AACCAGGCGC CTGGGCCAAC TCCCGATACG 
AAATCTTTAC CCGAAGTAAA CAGAGCCCGA ATGCATCTGA TTTTAGGTGT CAGTATACAG 
TTCCTCTGCT CCCCAAGACC CGAAGAACCT ATTGAACACG TTACAGCCTG TCTTCAAGCA 
CTCCGTACCT TACTGGACTC TCCCTGTGCC AGAATCCATA TTGCAGAAGA TCAGCTCGTT 
GGCGTGGAGT TGCTGAGTGT GGTACATCGA CTCCTTTTGA CCTGGAATCC GCCGTCGGTC 
CAGTTGCTGG TTACCGGAGT AGTGCAACAG ATCGTAAGGG CGGCTCAGGA CTATTTGCAG 
GAAAAAAGGA ACACGCTAAA TGAAGAGGAT TTAGAAAAAG AGACCTGCCC CACGTTAGGG 
GAAGGGGGTG ACGGCGGTGG TCTCGTCCCG GGGAAATCTC TTGTCTTTGC TACTATGGAA 
CTGCTGATGT TTATCTTGGT GAGGCACATG CCGCATCTCA GCACAAAAAT GTCCGACTCG 
CCGAGTCACG CCGGCACCAA AACGCCGTTG TCAGAAGAGA GCGCCCGACT GGTGGCTGCC 
ACGGTAACCA TCCTCTCGGA CCTGCCGTCG CTCTGTTCCC CTGCAGGCTG TATGACGATA 
CTCCCCACGA TCCTGTTTCT CATTGCGAGA ATCTTGAAGG ACACGGCAAT TAAGTCGGCG 
GATAATCAGG TCCCCCCTCC GGTCAGCGCG GCTTTACAGG GGATTAAAAA TATCGTGACT 
CTCCCACTGG TGAAGACCGA CGAAACGAGC AAGCAGTGGA CCGAACTGAT TCGCAGCACT 
CTCGCTTGTA TCCTGGAATA TTCTCAACCA GATGAGTCTT TGCCCACTCT TGACGAAGTG 
AGCATGCTCA CAGCTATCGC ACTCTTCCTG TGGTCCGCAA GTACTGAAAT AATAGGAGTC 
CAGTCGTTAC AGAACGGCTG CATGAACAGA TTTAAGAACG CGTTGAATTC CTGCGATCCG 
TGGGTTCAGG CCAAATGTTA CCAACTTCTC CTTTCACTGT TCCAACACTC CAATCGGGCC 
CTCTCAACTC CGTACATCCA TTCATTGGCC CCGATAATGG TTGAAAAACT GAAAGCCGTG 
GAGAGGAACC GGCCAAACAG CAACATAGAA CTTTTAGCGG TCCAAGAAGG GATCAAAGTC 
CTCGAAACCT TGGTTGCCCT TGGTGAAGAG CAGAACAGAG TGCAGCTGCT TGCCCTCTTG 
GTTCCCACTT TGATCTCTTA TCTACTGGAT GAAAACGCTT TTGCTTCGGC ACCTCTGGCC 
TCCAGAGATC TGCACGAGTT TGCCCTGCAG AATCTGATGC GTATCGGACC CCTCTACCCT 
CACGCTTTCA AGACGGTAAT GGGAGCCGCT CCCGAGTTGA AGATCCGTCT GGAAACTGCA 
GTGAGAGCGA GCCAGGCCAG CAAAGCCAAG GCAGCCGCCC GGCAGCCGGC CCCCACCGCA 
CACTCTGCCC CGACGATCAA ACTCAAGACG AGCTTTTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028905875.1
CDS	130..6351
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAGYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLKKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENVATLCFKALESSNYGVRVAVSKLLGTVMATALMPKQAAVMRQNVKRATLEEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERNFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEIVVNDTSGENKSGAAEIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHIEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESGGSNIDPFAAGVASRRDLHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASGDMSAANPFGGLKDEESEKKDEMDDDTMFTTLGEDEKSKPSVAPRWATRVFAADCLCRIILLCENADQAHFDLALARSAKLRNPKNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQTLEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMKIKREAEIKPKRAVKSTEEDDDDYGAADELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAAALWLNSTGFDTPEVAEEAAAAAVSGSQKRPTSVPLNQAPGPTPDTKSLPEVNRARMHLILGVSIQFLCSPRPEEPIEHVTACLQALRTLLDSPCARIHIAEDQLVGVELLSVVHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEEDLEKETCPTLGEGGDGGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHAGTKTPLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKNIVTLPLVKTDETSKQWTELIRSTLACILEYSQPDESLPTLDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWVQAKCYQLLLSLFQHSNRALSTPYIHSLAPIMVEKLKAVERNRPNSNIELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENAFASAPLASRDLHEFALQNLMRIGPLYPHAFKTVMGAAPELKIRLETAVRASQASKAKAAARQPAPTAHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_029050042.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050042.1

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT AGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAGCAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
AATATCGATC CTTTTGCAGC AGGAGTAGCC TCTCGGAGGG ATCTCCACTG CCGGCACCAG 
GGCGTCAACA TAACGGAGAC CGGCCTCGAA GGACTGCTTT TTGGGATGCT GGACCGCGAG 
ACGGATCGAA AACTCTGCTC CGACATCCAC GATACCCTGG GACATATGCT TTCGTCTCTG 
GCAGTCGAAA AGCTCTCGCA TTGGTTGATG CTGTGTAAAG ATGTTCTCGC CGCTTCCGGT 
GACATGAGCG CCGCGAATCC TTTCGGGGGA CTGAAGGATG AGGAATCCGA AAAAAAGGAC 
GAGATGGATG ACGATACCAT GTTCACCACC CTGGGAGAAG ACGAGAAGTC CAAACCTTCT 
GTAGCTCCTC GCTGGGCCAC TCGTGTATTT GCCGCTGACT GCCTGTGTCG AATCATCTTG 
CTCTGCGAGA ACGCTGACCA GGCCCATTTC GATCTCGCCC TAGCCCGTTC GGCCAAACTT 
CGGAACCCAA AAAATGACCT CTTAGTGCTC CACCTCTCCG ACCTGATCCG TATGGCATTT 
ATGGCTGCCA CGGATCATAG CAACCAGCTG CGGATGGCTG GACTCCAAAC CCTCGAGGAC 
ATTATCAAGA AGTTTGCATC TGTGCCCGAG CCAGAATTTC CAGGCCACGT AATATTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTTTA AGACCAGCTT TTTCACAAGA TACTCCATCA 
GATATAATAG CAAAAGCTTG CCAGGTCTGC AGTACCTGGA TTGGCAGCGG AGTCGTAAGC 
GACCTCAATG ACCTTCGCCG AGTCCACAAT TTGCTGGTCT CCTCCCTGGA CAAAGTACAG 
GCGGGAAAAG GGTCTTCAAG CCAACTTTAT AGAGAGAGTG CCACCACCAT GGAAAAACTG 
GCTGTCCTGA AAGCTTGGGC TGAGGTCTAC GTCGTTGCGA TGAAAATTAA GAGGGAAGCC 
GAGATAAAGC CAAAAAGAGC CGTTAAGAGC ACGGAGGAAG ACGACGACGA TTATGGGGCT 
GCAGATGAGC TGCCTCCAGA CAGCTTAATA ACCCTGGTGC AGCCCGAGCT GCCTATACTT 
AGCCGCCTTT GGTTAGCGGC GTTAAAAGAC TACGCGCTTC TGACTCTGCC CGCTGAATTC 
GCCAGCCAGC TCCCGCCAGA TGGCGGGGCC TTTTATACTC CCGAGACCAT CGATACCGCT 
AGGCTTCACT ATCGGAATTC TTGGGCTCCA ATTCTCCACG CAGCGGCCCT CTGGCTGAAC 
AGCACGGGAT TCGACACCCC GGAGGTCGCG GAAGAAGCGG CGGCGGCCGC CGTCTCCGGT 
TCTCAGAAGC GTCCTACGTC CGTCCCGTTA AACCAGGCGC CTGGGCCAAC TCCCGATACG 
AAATCTTTAC CCGAAGTAAA CAGAGCCCGA ATGCATCTGA TTTTAGGTGT CAGTATACAG 
TTCCTCTGCT CCCCAAGACC CGAAGAACCT ATTGAACACG TTACAGCCTG TCTTCAAGCA 
CTCCGTACCT TACTGGACTC TCCCTGTGCC AGAATCCATA TTGCAGAAGA TCAGCTCGTT 
GGCGTGGAGT TGCTGAGTGT GGTACATCGA CTCCTTTTGA CCTGGAATCC GCCGTCGGTC 
CAGTTGCTGG TTACCGGAGT AGTGCAACAG ATCGTAAGGG CGGCTCAGGA CTATTTGCAG 
GAAAAAAGGA ACACGCTAAA TGAAGAGGAT TTAGAAAAAG AGACCTGCCC CACGTTAGGG 
GAAGGGGGTG ACGGCGGTGG TCTCGTCCCG GGGAAATCTC TTGTCTTTGC TACTATGGAA 
CTGCTGATGT TTATCTTGGT GAGGCACATG CCGCATCTCA GCACAAAAAT GTCCGACTCG 
CCGAGTCACG CCGGCACCAA AACGCCGTTG TCAGAAGAGA GCGCCCGACT GGTGGCTGCC 
ACGGTAACCA TCCTCTCGGA CCTGCCGTCG CTCTGTTCCC CTGCAGGCTG TATGACGATA 
CTCCCCACGA TCCTGTTTCT CATTGCGAGA ATCTTGAAGG ACACGGCAAT TAAGTCGGCG 
GATAATCAGG TCCCCCCTCC GGTCAGCGCG GCTTTACAGG GGATTAAAAA TATCGTGACT 
CTCCCACTGG TGAAGACCGA CGAAACGAGC AAGCAGTGGA CCGAACTGAT TCGCAGCACT 
CTCGCTTGTA TCCTGGAATA TTCTCAACCA GATGAGTCTT TGCCCACTCT TGACGAAGTG 
AGCATGCTCA CAGCTATCGC ACTCTTCCTG TGGTCCGCAA GTACTGAAAT AATAGGAGTC 
CAGTCGTTAC AGAACGGCTG CATGAACAGA TTTAAGAACG CGTTGAATTC CTGCGATCCG 
TGGGTTCAGG CCAAATGTTA CCAACTTCTC CTTTCACTGT TCCAACACTC CAATCGGGCC 
CTCTCAACTC CGTACATCCA TTCATTGGCC CCGATAATGG TTGAAAAACT GAAAGCCGTG 
GAGAGGAACC GGCCAAACAG CAACATAGAA CTTTTAGCGG TCCAAGAAGG GATCAAAGTC 
CTCGAAACCT TGGTTGCCCT TGGTGAAGAG CAGAACAGAG TGCAGCTGCT TGCCCTCTTG 
GTTCCCACTT TGATCTCTTA TCTACTGGAT GAAAACGCTT TTGCTTCGGC ACCTCTGGCC 
TCCAGAGATC TGCACGAGTT TGCCCTGCAG AATCTGATGC GTATCGGACC CCTCTACCCT 
CACGCTTTCA AGACGGTAAT GGGAGCCGCT CCCGAGTTGA AGATCCGTCT GGAAACTGCA 
GTGAGAGCGA GCCAGGCCAG CAAAGCCAAG GCAGCCGCCC GGCAGCCGGC CCCCACCGCA 
CACTCTGCCC CGACGATCAA ACTCAAGACG AGCTTTTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_029050043.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028905876.1 Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

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CloneID	OOc135026
Clone ID Related Accession (Same CDS sequence)	XM_029050042.1 , XM_029050043.1
Accession Version	XM_029050043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	HEAT repeat-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT AGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAGCAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
AATATCGATC CTTTTGCAGC AGGAGTAGCC TCTCGGAGGG ATCTCCACTG CCGGCACCAG 
GGCGTCAACA TAACGGAGAC CGGCCTCGAA GGACTGCTTT TTGGGATGCT GGACCGCGAG 
ACGGATCGAA AACTCTGCTC CGACATCCAC GATACCCTGG GACATATGCT TTCGTCTCTG 
GCAGTCGAAA AGCTCTCGCA TTGGTTGATG CTGTGTAAAG ATGTTCTCGC CGCTTCCGGT 
GACATGAGCG CCGCGAATCC TTTCGGGGGA CTGAAGGATG AGGAATCCGA AAAAAAGGAC 
GAGATGGATG ACGATACCAT GTTCACCACC CTGGGAGAAG ACGAGAAGTC CAAACCTTCT 
GTAGCTCCTC GCTGGGCCAC TCGTGTATTT GCCGCTGACT GCCTGTGTCG AATCATCTTG 
CTCTGCGAGA ACGCTGACCA GGCCCATTTC GATCTCGCCC TAGCCCGTTC GGCCAAACTT 
CGGAACCCAA AAAATGACCT CTTAGTGCTC CACCTCTCCG ACCTGATCCG TATGGCATTT 
ATGGCTGCCA CGGATCATAG CAACCAGCTG CGGATGGCTG GACTCCAAAC CCTCGAGGAC 
ATTATCAAGA AGTTTGCATC TGTGCCCGAG CCAGAATTTC CAGGCCACGT AATATTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTTTA AGACCAGCTT TTTCACAAGA TACTCCATCA 
GATATAATAG CAAAAGCTTG CCAGGTCTGC AGTACCTGGA TTGGCAGCGG AGTCGTAAGC 
GACCTCAATG ACCTTCGCCG AGTCCACAAT TTGCTGGTCT CCTCCCTGGA CAAAGTACAG 
GCGGGAAAAG GGTCTTCAAG CCAACTTTAT AGAGAGAGTG CCACCACCAT GGAAAAACTG 
GCTGTCCTGA AAGCTTGGGC TGAGGTCTAC GTCGTTGCGA TGAAAATTAA GAGGGAAGCC 
GAGATAAAGC CAAAAAGAGC CGTTAAGAGC ACGGAGGAAG ACGACGACGA TTATGGGGCT 
GCAGATGAGC TGCCTCCAGA CAGCTTAATA ACCCTGGTGC AGCCCGAGCT GCCTATACTT 
AGCCGCCTTT GGTTAGCGGC GTTAAAAGAC TACGCGCTTC TGACTCTGCC CGCTGAATTC 
GCCAGCCAGC TCCCGCCAGA TGGCGGGGCC TTTTATACTC CCGAGACCAT CGATACCGCT 
AGGCTTCACT ATCGGAATTC TTGGGCTCCA ATTCTCCACG CAGCGGCCCT CTGGCTGAAC 
AGCACGGGAT TCGACACCCC GGAGGTCGCG GAAGAAGCGG CGGCGGCCGC CGTCTCCGGT 
TCTCAGAAGC GTCCTACGTC CGTCCCGTTA AACCAGGCGC CTGGGCCAAC TCCCGATACG 
AAATCTTTAC CCGAAGTAAA CAGAGCCCGA ATGCATCTGA TTTTAGGTGT CAGTATACAG 
TTCCTCTGCT CCCCAAGACC CGAAGAACCT ATTGAACACG TTACAGCCTG TCTTCAAGCA 
CTCCGTACCT TACTGGACTC TCCCTGTGCC AGAATCCATA TTGCAGAAGA TCAGCTCGTT 
GGCGTGGAGT TGCTGAGTGT GGTACATCGA CTCCTTTTGA CCTGGAATCC GCCGTCGGTC 
CAGTTGCTGG TTACCGGAGT AGTGCAACAG ATCGTAAGGG CGGCTCAGGA CTATTTGCAG 
GAAAAAAGGA ACACGCTAAA TGAAGAGGAT TTAGAAAAAG AGACCTGCCC CACGTTAGGG 
GAAGGGGGTG ACGGCGGTGG TCTCGTCCCG GGGAAATCTC TTGTCTTTGC TACTATGGAA 
CTGCTGATGT TTATCTTGGT GAGGCACATG CCGCATCTCA GCACAAAAAT GTCCGACTCG 
CCGAGTCACG CCGGCACCAA AACGCCGTTG TCAGAAGAGA GCGCCCGACT GGTGGCTGCC 
ACGGTAACCA TCCTCTCGGA CCTGCCGTCG CTCTGTTCCC CTGCAGGCTG TATGACGATA 
CTCCCCACGA TCCTGTTTCT CATTGCGAGA ATCTTGAAGG ACACGGCAAT TAAGTCGGCG 
GATAATCAGG TCCCCCCTCC GGTCAGCGCG GCTTTACAGG GGATTAAAAA TATCGTGACT 
CTCCCACTGG TGAAGACCGA CGAAACGAGC AAGCAGTGGA CCGAACTGAT TCGCAGCACT 
CTCGCTTGTA TCCTGGAATA TTCTCAACCA GATGAGTCTT TGCCCACTCT TGACGAAGTG 
AGCATGCTCA CAGCTATCGC ACTCTTCCTG TGGTCCGCAA GTACTGAAAT AATAGGAGTC 
CAGTCGTTAC AGAACGGCTG CATGAACAGA TTTAAGAACG CGTTGAATTC CTGCGATCCG 
TGGGTTCAGG CCAAATGTTA CCAACTTCTC CTTTCACTGT TCCAACACTC CAATCGGGCC 
CTCTCAACTC CGTACATCCA TTCATTGGCC CCGATAATGG TTGAAAAACT GAAAGCCGTG 
GAGAGGAACC GGCCAAACAG CAACATAGAA CTTTTAGCGG TCCAAGAAGG GATCAAAGTC 
CTCGAAACCT TGGTTGCCCT TGGTGAAGAG CAGAACAGAG TGCAGCTGCT TGCCCTCTTG 
GTTCCCACTT TGATCTCTTA TCTACTGGAT GAAAACGCTT TTGCTTCGGC ACCTCTGGCC 
TCCAGAGATC TGCACGAGTT TGCCCTGCAG AATCTGATGC GTATCGGACC CCTCTACCCT 
CACGCTTTCA AGACGGTAAT GGGAGCCGCT CCCGAGTTGA AGATCCGTCT GGAAACTGCA 
GTGAGAGCGA GCCAGGCCAG CAAAGCCAAG GCAGCCGCCC GGCAGCCGGC CCCCACCGCA 
CACTCTGCCC CGACGATCAA ACTCAAGACG AGCTTTTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028905876.1
CDS	120..6341
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAGYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLKKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENVATLCFKALESSNYGVRVAVSKLLGTVMATALMPKQAAVMRQNVKRATLEEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERNFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEIVVNDTSGENKSGAAEIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHIEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESGGSNIDPFAAGVASRRDLHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASGDMSAANPFGGLKDEESEKKDEMDDDTMFTTLGEDEKSKPSVAPRWATRVFAADCLCRIILLCENADQAHFDLALARSAKLRNPKNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQTLEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMKIKREAEIKPKRAVKSTEEDDDDYGAADELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAAALWLNSTGFDTPEVAEEAAAAAVSGSQKRPTSVPLNQAPGPTPDTKSLPEVNRARMHLILGVSIQFLCSPRPEEPIEHVTACLQALRTLLDSPCARIHIAEDQLVGVELLSVVHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEEDLEKETCPTLGEGGDGGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHAGTKTPLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKNIVTLPLVKTDETSKQWTELIRSTLACILEYSQPDESLPTLDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWVQAKCYQLLLSLFQHSNRALSTPYIHSLAPIMVEKLKAVERNRPNSNIELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENAFASAPLASRDLHEFALQNLMRIGPLYPHAFKTVMGAAPELKIRLETAVRASQASKAKAAARQPAPTAHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_029050043.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050043.1

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT AGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAGCAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
AATATCGATC CTTTTGCAGC AGGAGTAGCC TCTCGGAGGG ATCTCCACTG CCGGCACCAG 
GGCGTCAACA TAACGGAGAC CGGCCTCGAA GGACTGCTTT TTGGGATGCT GGACCGCGAG 
ACGGATCGAA AACTCTGCTC CGACATCCAC GATACCCTGG GACATATGCT TTCGTCTCTG 
GCAGTCGAAA AGCTCTCGCA TTGGTTGATG CTGTGTAAAG ATGTTCTCGC CGCTTCCGGT 
GACATGAGCG CCGCGAATCC TTTCGGGGGA CTGAAGGATG AGGAATCCGA AAAAAAGGAC 
GAGATGGATG ACGATACCAT GTTCACCACC CTGGGAGAAG ACGAGAAGTC CAAACCTTCT 
GTAGCTCCTC GCTGGGCCAC TCGTGTATTT GCCGCTGACT GCCTGTGTCG AATCATCTTG 
CTCTGCGAGA ACGCTGACCA GGCCCATTTC GATCTCGCCC TAGCCCGTTC GGCCAAACTT 
CGGAACCCAA AAAATGACCT CTTAGTGCTC CACCTCTCCG ACCTGATCCG TATGGCATTT 
ATGGCTGCCA CGGATCATAG CAACCAGCTG CGGATGGCTG GACTCCAAAC CCTCGAGGAC 
ATTATCAAGA AGTTTGCATC TGTGCCCGAG CCAGAATTTC CAGGCCACGT AATATTGGAG 
CAGTATCAAG CTAATGTGGG AGCTGCTTTA AGACCAGCTT TTTCACAAGA TACTCCATCA 
GATATAATAG CAAAAGCTTG CCAGGTCTGC AGTACCTGGA TTGGCAGCGG AGTCGTAAGC 
GACCTCAATG ACCTTCGCCG AGTCCACAAT TTGCTGGTCT CCTCCCTGGA CAAAGTACAG 
GCGGGAAAAG GGTCTTCAAG CCAACTTTAT AGAGAGAGTG CCACCACCAT GGAAAAACTG 
GCTGTCCTGA AAGCTTGGGC TGAGGTCTAC GTCGTTGCGA TGAAAATTAA GAGGGAAGCC 
GAGATAAAGC CAAAAAGAGC CGTTAAGAGC ACGGAGGAAG ACGACGACGA TTATGGGGCT 
GCAGATGAGC TGCCTCCAGA CAGCTTAATA ACCCTGGTGC AGCCCGAGCT GCCTATACTT 
AGCCGCCTTT GGTTAGCGGC GTTAAAAGAC TACGCGCTTC TGACTCTGCC CGCTGAATTC 
GCCAGCCAGC TCCCGCCAGA TGGCGGGGCC TTTTATACTC CCGAGACCAT CGATACCGCT 
AGGCTTCACT ATCGGAATTC TTGGGCTCCA ATTCTCCACG CAGCGGCCCT CTGGCTGAAC 
AGCACGGGAT TCGACACCCC GGAGGTCGCG GAAGAAGCGG CGGCGGCCGC CGTCTCCGGT 
TCTCAGAAGC GTCCTACGTC CGTCCCGTTA AACCAGGCGC CTGGGCCAAC TCCCGATACG 
AAATCTTTAC CCGAAGTAAA CAGAGCCCGA ATGCATCTGA TTTTAGGTGT CAGTATACAG 
TTCCTCTGCT CCCCAAGACC CGAAGAACCT ATTGAACACG TTACAGCCTG TCTTCAAGCA 
CTCCGTACCT TACTGGACTC TCCCTGTGCC AGAATCCATA TTGCAGAAGA TCAGCTCGTT 
GGCGTGGAGT TGCTGAGTGT GGTACATCGA CTCCTTTTGA CCTGGAATCC GCCGTCGGTC 
CAGTTGCTGG TTACCGGAGT AGTGCAACAG ATCGTAAGGG CGGCTCAGGA CTATTTGCAG 
GAAAAAAGGA ACACGCTAAA TGAAGAGGAT TTAGAAAAAG AGACCTGCCC CACGTTAGGG 
GAAGGGGGTG ACGGCGGTGG TCTCGTCCCG GGGAAATCTC TTGTCTTTGC TACTATGGAA 
CTGCTGATGT TTATCTTGGT GAGGCACATG CCGCATCTCA GCACAAAAAT GTCCGACTCG 
CCGAGTCACG CCGGCACCAA AACGCCGTTG TCAGAAGAGA GCGCCCGACT GGTGGCTGCC 
ACGGTAACCA TCCTCTCGGA CCTGCCGTCG CTCTGTTCCC CTGCAGGCTG TATGACGATA 
CTCCCCACGA TCCTGTTTCT CATTGCGAGA ATCTTGAAGG ACACGGCAAT TAAGTCGGCG 
GATAATCAGG TCCCCCCTCC GGTCAGCGCG GCTTTACAGG GGATTAAAAA TATCGTGACT 
CTCCCACTGG TGAAGACCGA CGAAACGAGC AAGCAGTGGA CCGAACTGAT TCGCAGCACT 
CTCGCTTGTA TCCTGGAATA TTCTCAACCA GATGAGTCTT TGCCCACTCT TGACGAAGTG 
AGCATGCTCA CAGCTATCGC ACTCTTCCTG TGGTCCGCAA GTACTGAAAT AATAGGAGTC 
CAGTCGTTAC AGAACGGCTG CATGAACAGA TTTAAGAACG CGTTGAATTC CTGCGATCCG 
TGGGTTCAGG CCAAATGTTA CCAACTTCTC CTTTCACTGT TCCAACACTC CAATCGGGCC 
CTCTCAACTC CGTACATCCA TTCATTGGCC CCGATAATGG TTGAAAAACT GAAAGCCGTG 
GAGAGGAACC GGCCAAACAG CAACATAGAA CTTTTAGCGG TCCAAGAAGG GATCAAAGTC 
CTCGAAACCT TGGTTGCCCT TGGTGAAGAG CAGAACAGAG TGCAGCTGCT TGCCCTCTTG 
GTTCCCACTT TGATCTCTTA TCTACTGGAT GAAAACGCTT TTGCTTCGGC ACCTCTGGCC 
TCCAGAGATC TGCACGAGTT TGCCCTGCAG AATCTGATGC GTATCGGACC CCTCTACCCT 
CACGCTTTCA AGACGGTAAT GGGAGCCGCT CCCGAGTTGA AGATCCGTCT GGAAACTGCA 
GTGAGAGCGA GCCAGGCCAG CAAAGCCAAG GCAGCCGCCC GGCAGCCGGC CCCCACCGCA 
CACTCTGCCC CGACGATCAA ACTCAAGACG AGCTTTTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_029050044.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028905877.1 Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

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CloneID	OOc135027
Clone ID Related Accession (Same CDS sequence)	XM_029050044.1
Accession Version	XM_029050044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	HEAT repeat-containing protein 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT AGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAGCAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
ACAGGAGTAG CCTCTCGGAG GGATCTCCAC TGCCGGCACC AGGGCGTCAA CATAACGGAG 
ACCGGCCTCG AAGGACTGCT TTTTGGGATG CTGGACCGCG AGACGGATCG AAAACTCTGC 
TCCGACATCC ACGATACCCT GGGACATATG CTTTCGTCTC TGGCAGTCGA AAAGCTCTCG 
CATTGGTTGA TGCTGTGTAA AGATGTTCTC GCCGCTTCCG GTGACATGAG CGCCGCGAAT 
CCTTTCGGGG GACTGAAGGA TGAGGAATCC GAAAAAAAGG ACGAGATGGA TGACGATACC 
ATGTTCACCA CCCTGGGAGA AGACGAGAAG TCCAAACCTT CTGTAGCTCC TCGCTGGGCC 
ACTCGTGTAT TTGCCGCTGA CTGCCTGTGT CGAATCATCT TGCTCTGCGA GAACGCTGAC 
CAGGCCCATT TCGATCTCGC CCTAGCCCGT TCGGCCAAAC TTCGGAACCC AAAAAATGAC 
CTCTTAGTGC TCCACCTCTC CGACCTGATC CGTATGGCAT TTATGGCTGC CACGGATCAT 
AGCAACCAGC TGCGGATGGC TGGACTCCAA ACCCTCGAGG ACATTATCAA GAAGTTTGCA 
TCTGTGCCCG AGCCAGAATT TCCAGGCCAC GTAATATTGG AGCAGTATCA AGCTAATGTG 
GGAGCTGCTT TAAGACCAGC TTTTTCACAA GATACTCCAT CAGATATAAT AGCAAAAGCT 
TGCCAGGTCT GCAGTACCTG GATTGGCAGC GGAGTCGTAA GCGACCTCAA TGACCTTCGC 
CGAGTCCACA ATTTGCTGGT CTCCTCCCTG GACAAAGTAC AGGCGGGAAA AGGGTCTTCA 
AGCCAACTTT ATAGAGAGAG TGCCACCACC ATGGAAAAAC TGGCTGTCCT GAAAGCTTGG 
GCTGAGGTCT ACGTCGTTGC GATGAAAATT AAGAGGGAAG CCGAGATAAA GCCAAAAAGA 
GCCGTTAAGA GCACGGAGGA AGACGACGAC GATTATGGGG CTGCAGATGA GCTGCCTCCA 
GACAGCTTAA TAACCCTGGT GCAGCCCGAG CTGCCTATAC TTAGCCGCCT TTGGTTAGCG 
GCGTTAAAAG ACTACGCGCT TCTGACTCTG CCCGCTGAAT TCGCCAGCCA GCTCCCGCCA 
GATGGCGGGG CCTTTTATAC TCCCGAGACC ATCGATACCG CTAGGCTTCA CTATCGGAAT 
TCTTGGGCTC CAATTCTCCA CGCAGCGGCC CTCTGGCTGA ACAGCACGGG ATTCGACACC 
CCGGAGGTCG CGGAAGAAGC GGCGGCGGCC GCCGTCTCCG GTTCTCAGAA GCGTCCTACG 
TCCGTCCCGT TAAACCAGGC GCCTGGGCCA ACTCCCGATA CGAAATCTTT ACCCGAAGTA 
AACAGAGCCC GAATGCATCT GATTTTAGGT GTCAGTATAC AGTTCCTCTG CTCCCCAAGA 
CCCGAAGAAC CTATTGAACA CGTTACAGCC TGTCTTCAAG CACTCCGTAC CTTACTGGAC 
TCTCCCTGTG CCAGAATCCA TATTGCAGAA GATCAGCTCG TTGGCGTGGA GTTGCTGAGT 
GTGGTACATC GACTCCTTTT GACCTGGAAT CCGCCGTCGG TCCAGTTGCT GGTTACCGGA 
GTAGTGCAAC AGATCGTAAG GGCGGCTCAG GACTATTTGC AGGAAAAAAG GAACACGCTA 
AATGAAGAGG ATTTAGAAAA AGAGACCTGC CCCACGTTAG GGGAAGGGGG TGACGGCGGT 
GGTCTCGTCC CGGGGAAATC TCTTGTCTTT GCTACTATGG AACTGCTGAT GTTTATCTTG 
GTGAGGCACA TGCCGCATCT CAGCACAAAA ATGTCCGACT CGCCGAGTCA CGCCGGCACC 
AAAACGCCGT TGTCAGAAGA GAGCGCCCGA CTGGTGGCTG CCACGGTAAC CATCCTCTCG 
GACCTGCCGT CGCTCTGTTC CCCTGCAGGC TGTATGACGA TACTCCCCAC GATCCTGTTT 
CTCATTGCGA GAATCTTGAA GGACACGGCA ATTAAGTCGG CGGATAATCA GGTCCCCCCT 
CCGGTCAGCG CGGCTTTACA GGGGATTAAA AATATCGTGA CTCTCCCACT GGTGAAGACC 
GACGAAACGA GCAAGCAGTG GACCGAACTG ATTCGCAGCA CTCTCGCTTG TATCCTGGAA 
TATTCTCAAC CAGATGAGTC TTTGCCCACT CTTGACGAAG TGAGCATGCT CACAGCTATC 
GCACTCTTCC TGTGGTCCGC AAGTACTGAA ATAATAGGAG TCCAGTCGTT ACAGAACGGC 
TGCATGAACA GATTTAAGAA CGCGTTGAAT TCCTGCGATC CGTGGGTTCA GGCCAAATGT 
TACCAACTTC TCCTTTCACT GTTCCAACAC TCCAATCGGG CCCTCTCAAC TCCGTACATC 
CATTCATTGG CCCCGATAAT GGTTGAAAAA CTGAAAGCCG TGGAGAGGAA CCGGCCAAAC 
AGCAACATAG AACTTTTAGC GGTCCAAGAA GGGATCAAAG TCCTCGAAAC CTTGGTTGCC 
CTTGGTGAAG AGCAGAACAG AGTGCAGCTG CTTGCCCTCT TGGTTCCCAC TTTGATCTCT 
TATCTACTGG ATGAAAACGC TTTTGCTTCG GCACCTCTGG CCTCCAGAGA TCTGCACGAG 
TTTGCCCTGC AGAATCTGAT GCGTATCGGA CCCCTCTACC CTCACGCTTT CAAGACGGTA 
ATGGGAGCCG CTCCCGAGTT GAAGATCCGT CTGGAAACTG CAGTGAGAGC GAGCCAGGCC 
AGCAAAGCCA AGGCAGCCGC CCGGCAGCCG GCCCCCACCG CACACTCTGC CCCGACGATC 
AAACTCAAGA CGAGCTTTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028905877.1
CDS	130..6333
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAGYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLKKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENVATLCFKALESSNYGVRVAVSKLLGTVMATALMPKQAAVMRQNVKRATLEEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERNFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEIVVNDTSGENKSGAAEIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHIEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESGGSTGVASRRDLHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASGDMSAANPFGGLKDEESEKKDEMDDDTMFTTLGEDEKSKPSVAPRWATRVFAADCLCRIILLCENADQAHFDLALARSAKLRNPKNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQTLEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMKIKREAEIKPKRAVKSTEEDDDDYGAADELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAAALWLNSTGFDTPEVAEEAAAAAVSGSQKRPTSVPLNQAPGPTPDTKSLPEVNRARMHLILGVSIQFLCSPRPEEPIEHVTACLQALRTLLDSPCARIHIAEDQLVGVELLSVVHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEEDLEKETCPTLGEGGDGGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHAGTKTPLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKNIVTLPLVKTDETSKQWTELIRSTLACILEYSQPDESLPTLDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWVQAKCYQLLLSLFQHSNRALSTPYIHSLAPIMVEKLKAVERNRPNSNIELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENAFASAPLASRDLHEFALQNLMRIGPLYPHAFKTVMGAAPELKIRLETAVRASQASKAKAAARQPAPTAHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_029050044.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050044.1

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT AGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAGCAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
ACAGGAGTAG CCTCTCGGAG GGATCTCCAC TGCCGGCACC AGGGCGTCAA CATAACGGAG 
ACCGGCCTCG AAGGACTGCT TTTTGGGATG CTGGACCGCG AGACGGATCG AAAACTCTGC 
TCCGACATCC ACGATACCCT GGGACATATG CTTTCGTCTC TGGCAGTCGA AAAGCTCTCG 
CATTGGTTGA TGCTGTGTAA AGATGTTCTC GCCGCTTCCG GTGACATGAG CGCCGCGAAT 
CCTTTCGGGG GACTGAAGGA TGAGGAATCC GAAAAAAAGG ACGAGATGGA TGACGATACC 
ATGTTCACCA CCCTGGGAGA AGACGAGAAG TCCAAACCTT CTGTAGCTCC TCGCTGGGCC 
ACTCGTGTAT TTGCCGCTGA CTGCCTGTGT CGAATCATCT TGCTCTGCGA GAACGCTGAC 
CAGGCCCATT TCGATCTCGC CCTAGCCCGT TCGGCCAAAC TTCGGAACCC AAAAAATGAC 
CTCTTAGTGC TCCACCTCTC CGACCTGATC CGTATGGCAT TTATGGCTGC CACGGATCAT 
AGCAACCAGC TGCGGATGGC TGGACTCCAA ACCCTCGAGG ACATTATCAA GAAGTTTGCA 
TCTGTGCCCG AGCCAGAATT TCCAGGCCAC GTAATATTGG AGCAGTATCA AGCTAATGTG 
GGAGCTGCTT TAAGACCAGC TTTTTCACAA GATACTCCAT CAGATATAAT AGCAAAAGCT 
TGCCAGGTCT GCAGTACCTG GATTGGCAGC GGAGTCGTAA GCGACCTCAA TGACCTTCGC 
CGAGTCCACA ATTTGCTGGT CTCCTCCCTG GACAAAGTAC AGGCGGGAAA AGGGTCTTCA 
AGCCAACTTT ATAGAGAGAG TGCCACCACC ATGGAAAAAC TGGCTGTCCT GAAAGCTTGG 
GCTGAGGTCT ACGTCGTTGC GATGAAAATT AAGAGGGAAG CCGAGATAAA GCCAAAAAGA 
GCCGTTAAGA GCACGGAGGA AGACGACGAC GATTATGGGG CTGCAGATGA GCTGCCTCCA 
GACAGCTTAA TAACCCTGGT GCAGCCCGAG CTGCCTATAC TTAGCCGCCT TTGGTTAGCG 
GCGTTAAAAG ACTACGCGCT TCTGACTCTG CCCGCTGAAT TCGCCAGCCA GCTCCCGCCA 
GATGGCGGGG CCTTTTATAC TCCCGAGACC ATCGATACCG CTAGGCTTCA CTATCGGAAT 
TCTTGGGCTC CAATTCTCCA CGCAGCGGCC CTCTGGCTGA ACAGCACGGG ATTCGACACC 
CCGGAGGTCG CGGAAGAAGC GGCGGCGGCC GCCGTCTCCG GTTCTCAGAA GCGTCCTACG 
TCCGTCCCGT TAAACCAGGC GCCTGGGCCA ACTCCCGATA CGAAATCTTT ACCCGAAGTA 
AACAGAGCCC GAATGCATCT GATTTTAGGT GTCAGTATAC AGTTCCTCTG CTCCCCAAGA 
CCCGAAGAAC CTATTGAACA CGTTACAGCC TGTCTTCAAG CACTCCGTAC CTTACTGGAC 
TCTCCCTGTG CCAGAATCCA TATTGCAGAA GATCAGCTCG TTGGCGTGGA GTTGCTGAGT 
GTGGTACATC GACTCCTTTT GACCTGGAAT CCGCCGTCGG TCCAGTTGCT GGTTACCGGA 
GTAGTGCAAC AGATCGTAAG GGCGGCTCAG GACTATTTGC AGGAAAAAAG GAACACGCTA 
AATGAAGAGG ATTTAGAAAA AGAGACCTGC CCCACGTTAG GGGAAGGGGG TGACGGCGGT 
GGTCTCGTCC CGGGGAAATC TCTTGTCTTT GCTACTATGG AACTGCTGAT GTTTATCTTG 
GTGAGGCACA TGCCGCATCT CAGCACAAAA ATGTCCGACT CGCCGAGTCA CGCCGGCACC 
AAAACGCCGT TGTCAGAAGA GAGCGCCCGA CTGGTGGCTG CCACGGTAAC CATCCTCTCG 
GACCTGCCGT CGCTCTGTTC CCCTGCAGGC TGTATGACGA TACTCCCCAC GATCCTGTTT 
CTCATTGCGA GAATCTTGAA GGACACGGCA ATTAAGTCGG CGGATAATCA GGTCCCCCCT 
CCGGTCAGCG CGGCTTTACA GGGGATTAAA AATATCGTGA CTCTCCCACT GGTGAAGACC 
GACGAAACGA GCAAGCAGTG GACCGAACTG ATTCGCAGCA CTCTCGCTTG TATCCTGGAA 
TATTCTCAAC CAGATGAGTC TTTGCCCACT CTTGACGAAG TGAGCATGCT CACAGCTATC 
GCACTCTTCC TGTGGTCCGC AAGTACTGAA ATAATAGGAG TCCAGTCGTT ACAGAACGGC 
TGCATGAACA GATTTAAGAA CGCGTTGAAT TCCTGCGATC CGTGGGTTCA GGCCAAATGT 
TACCAACTTC TCCTTTCACT GTTCCAACAC TCCAATCGGG CCCTCTCAAC TCCGTACATC 
CATTCATTGG CCCCGATAAT GGTTGAAAAA CTGAAAGCCG TGGAGAGGAA CCGGCCAAAC 
AGCAACATAG AACTTTTAGC GGTCCAAGAA GGGATCAAAG TCCTCGAAAC CTTGGTTGCC 
CTTGGTGAAG AGCAGAACAG AGTGCAGCTG CTTGCCCTCT TGGTTCCCAC TTTGATCTCT 
TATCTACTGG ATGAAAACGC TTTTGCTTCG GCACCTCTGG CCTCCAGAGA TCTGCACGAG 
TTTGCCCTGC AGAATCTGAT GCGTATCGGA CCCCTCTACC CTCACGCTTT CAAGACGGTA 
ATGGGAGCCG CTCCCGAGTT GAAGATCCGT CTGGAAACTG CAGTGAGAGC GAGCCAGGCC 
AGCAAAGCCA AGGCAGCCGC CCGGCAGCCG GCCCCCACCG CACACTCTGC CCCGACGATC 
AAACTCAAGA CGAGCTTTTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5B ( XM_029050045.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028905878.1 Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X4, mRNA.

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CloneID	OOc135028
Clone ID Related Accession (Same CDS sequence)	XM_029050045.1
Accession Version	XM_029050045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-28
Organism	Ornithorhynchus anatinus(platypus)
Product	HEAT repeat-containing protein 5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041749.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT AGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAGCAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
AGAGTAGCCT CTCGGAGGGA TCTCCACTGC CGGCACCAGG GCGTCAACAT AACGGAGACC 
GGCCTCGAAG GACTGCTTTT TGGGATGCTG GACCGCGAGA CGGATCGAAA ACTCTGCTCC 
GACATCCACG ATACCCTGGG ACATATGCTT TCGTCTCTGG CAGTCGAAAA GCTCTCGCAT 
TGGTTGATGC TGTGTAAAGA TGTTCTCGCC GCTTCCGGTG ACATGAGCGC CGCGAATCCT 
TTCGGGGGAC TGAAGGATGA GGAATCCGAA AAAAAGGACG AGATGGATGA CGATACCATG 
TTCACCACCC TGGGAGAAGA CGAGAAGTCC AAACCTTCTG TAGCTCCTCG CTGGGCCACT 
CGTGTATTTG CCGCTGACTG CCTGTGTCGA ATCATCTTGC TCTGCGAGAA CGCTGACCAG 
GCCCATTTCG ATCTCGCCCT AGCCCGTTCG GCCAAACTTC GGAACCCAAA AAATGACCTC 
TTAGTGCTCC ACCTCTCCGA CCTGATCCGT ATGGCATTTA TGGCTGCCAC GGATCATAGC 
AACCAGCTGC GGATGGCTGG ACTCCAAACC CTCGAGGACA TTATCAAGAA GTTTGCATCT 
GTGCCCGAGC CAGAATTTCC AGGCCACGTA ATATTGGAGC AGTATCAAGC TAATGTGGGA 
GCTGCTTTAA GACCAGCTTT TTCACAAGAT ACTCCATCAG ATATAATAGC AAAAGCTTGC 
CAGGTCTGCA GTACCTGGAT TGGCAGCGGA GTCGTAAGCG ACCTCAATGA CCTTCGCCGA 
GTCCACAATT TGCTGGTCTC CTCCCTGGAC AAAGTACAGG CGGGAAAAGG GTCTTCAAGC 
CAACTTTATA GAGAGAGTGC CACCACCATG GAAAAACTGG CTGTCCTGAA AGCTTGGGCT 
GAGGTCTACG TCGTTGCGAT GAAAATTAAG AGGGAAGCCG AGATAAAGCC AAAAAGAGCC 
GTTAAGAGCA CGGAGGAAGA CGACGACGAT TATGGGGCTG CAGATGAGCT GCCTCCAGAC 
AGCTTAATAA CCCTGGTGCA GCCCGAGCTG CCTATACTTA GCCGCCTTTG GTTAGCGGCG 
TTAAAAGACT ACGCGCTTCT GACTCTGCCC GCTGAATTCG CCAGCCAGCT CCCGCCAGAT 
GGCGGGGCCT TTTATACTCC CGAGACCATC GATACCGCTA GGCTTCACTA TCGGAATTCT 
TGGGCTCCAA TTCTCCACGC AGCGGCCCTC TGGCTGAACA GCACGGGATT CGACACCCCG 
GAGGTCGCGG AAGAAGCGGC GGCGGCCGCC GTCTCCGGTT CTCAGAAGCG TCCTACGTCC 
GTCCCGTTAA ACCAGGCGCC TGGGCCAACT CCCGATACGA AATCTTTACC CGAAGTAAAC 
AGAGCCCGAA TGCATCTGAT TTTAGGTGTC AGTATACAGT TCCTCTGCTC CCCAAGACCC 
GAAGAACCTA TTGAACACGT TACAGCCTGT CTTCAAGCAC TCCGTACCTT ACTGGACTCT 
CCCTGTGCCA GAATCCATAT TGCAGAAGAT CAGCTCGTTG GCGTGGAGTT GCTGAGTGTG 
GTACATCGAC TCCTTTTGAC CTGGAATCCG CCGTCGGTCC AGTTGCTGGT TACCGGAGTA 
GTGCAACAGA TCGTAAGGGC GGCTCAGGAC TATTTGCAGG AAAAAAGGAA CACGCTAAAT 
GAAGAGGATT TAGAAAAAGA GACCTGCCCC ACGTTAGGGG AAGGGGGTGA CGGCGGTGGT 
CTCGTCCCGG GGAAATCTCT TGTCTTTGCT ACTATGGAAC TGCTGATGTT TATCTTGGTG 
AGGCACATGC CGCATCTCAG CACAAAAATG TCCGACTCGC CGAGTCACGC CGGCACCAAA 
ACGCCGTTGT CAGAAGAGAG CGCCCGACTG GTGGCTGCCA CGGTAACCAT CCTCTCGGAC 
CTGCCGTCGC TCTGTTCCCC TGCAGGCTGT ATGACGATAC TCCCCACGAT CCTGTTTCTC 
ATTGCGAGAA TCTTGAAGGA CACGGCAATT AAGTCGGCGG ATAATCAGGT CCCCCCTCCG 
GTCAGCGCGG CTTTACAGGG GATTAAAAAT ATCGTGACTC TCCCACTGGT GAAGACCGAC 
GAAACGAGCA AGCAGTGGAC CGAACTGATT CGCAGCACTC TCGCTTGTAT CCTGGAATAT 
TCTCAACCAG ATGAGTCTTT GCCCACTCTT GACGAAGTGA GCATGCTCAC AGCTATCGCA 
CTCTTCCTGT GGTCCGCAAG TACTGAAATA ATAGGAGTCC AGTCGTTACA GAACGGCTGC 
ATGAACAGAT TTAAGAACGC GTTGAATTCC TGCGATCCGT GGGTTCAGGC CAAATGTTAC 
CAACTTCTCC TTTCACTGTT CCAACACTCC AATCGGGCCC TCTCAACTCC GTACATCCAT 
TCATTGGCCC CGATAATGGT TGAAAAACTG AAAGCCGTGG AGAGGAACCG GCCAAACAGC 
AACATAGAAC TTTTAGCGGT CCAAGAAGGG ATCAAAGTCC TCGAAACCTT GGTTGCCCTT 
GGTGAAGAGC AGAACAGAGT GCAGCTGCTT GCCCTCTTGG TTCCCACTTT GATCTCTTAT 
CTACTGGATG AAAACGCTTT TGCTTCGGCA CCTCTGGCCT CCAGAGATCT GCACGAGTTT 
GCCCTGCAGA ATCTGATGCG TATCGGACCC CTCTACCCTC ACGCTTTCAA GACGGTAATG 
GGAGCCGCTC CCGAGTTGAA GATCCGTCTG GAAACTGCAG TGAGAGCGAG CCAGGCCAGC 
AAAGCCAAGG CAGCCGCCCG GCAGCCGGCC CCCACCGCAC ACTCTGCCCC GACGATCAAA 
CTCAAGACGA GCTTTTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028905878.1
CDS	130..6330
Translation	MELAHSLLLNEEALAQITEAKRPVFIFEWLRFLDKVLVAANKTDVKEKQKKLVEQLTGLISSSPGPPTRKLLAKNLAALYSIGDTFTVFQTLDKCNDIIKNKDDTAGYLPTKLAAVACVGAFYEKMGRMLGSAFPETVNNLLKSLKSAESQGRSEILMSLKKVLNGLGGAAASCHRDIYKNARSLLTDRSMAVRCAVAKCLLELQNEAVFMWTAELENVATLCFKALESSNYGVRVAVSKLLGTVMATALMPKQAAVMRQNVKRATLEEVLELMATGFLRGGSGFLKSGGEMLKVGGSVNREVRVGVTQAYVVFVTTLGGQWLERNFATFLSHVLDLVSHPRATQTHVEAVYSRRCVSFILRATVGSLLGEKAQIAAAKEICQAIGKQMKAVEIVVNDTSGENKSGAAEIAASQHVMVCALQELGSLVQSLNATASPLIQEASIGLLETVTSVLLHPSMAARLAAAWCLRCVAVALPFQLTPFLDRCAERLNNLKTSPEAVSGYSFAMAALLGGVHQCPLGIPHAKGKMVVSIAEDLLRTAAQNSRLSLQRTQAGWLLLGALMTLGPSVVRYHLPKMLLLWRNVFPRSLKELEAEKARGDSFTWQVTLEGRAGALCAMRSFVAHCPELLTEDVIRKLMTPIECAMTMMSHIPSVIKAHGAHLKASAAMVRLRLYDILALLPPKTYEGSFNALLRELVAEFTLTDNSANTTTSLLRSLCHYDDSVLLGSWLQETDHKSIEDQLQPNSASGSGALEHDPSSIYLRIPAGEAVPGPLPLGVSVIDASVALFGVVFPHVSYKHRLQMLDHFAECVKQAKGVRQQAVQLNIFTAVLSALKGLAENKSTLGPEEVRKSALTLVMGALDNPNPILRCAAGEALGRMAQVVGEATFIARMAQYSFDKLKSARDVVSRTGHSLALGCLHRYVGGIGSGQHLKTSVSILLALAQDGTSPEVQTWSLHSLALIVDSSGPMYRGYVEPTLSLVLTLLLTVPPSHIEVHQCLGRCLGAIITTVGPELQGNGATISTIRSSCLVGCAITQDHSDSLVQAAAISCLQQLHMFAPRHVNLSSLVPSLCVHLCSSHLLLRRAAVACLRQLAQREAAEVCEYAMSLAKNAGDKESGGSRVASRRDLHCRHQGVNITETGLEGLLFGMLDRETDRKLCSDIHDTLGHMLSSLAVEKLSHWLMLCKDVLAASGDMSAANPFGGLKDEESEKKDEMDDDTMFTTLGEDEKSKPSVAPRWATRVFAADCLCRIILLCENADQAHFDLALARSAKLRNPKNDLLVLHLSDLIRMAFMAATDHSNQLRMAGLQTLEDIIKKFASVPEPEFPGHVILEQYQANVGAALRPAFSQDTPSDIIAKACQVCSTWIGSGVVSDLNDLRRVHNLLVSSLDKVQAGKGSSSQLYRESATTMEKLAVLKAWAEVYVVAMKIKREAEIKPKRAVKSTEEDDDDYGAADELPPDSLITLVQPELPILSRLWLAALKDYALLTLPAEFASQLPPDGGAFYTPETIDTARLHYRNSWAPILHAAALWLNSTGFDTPEVAEEAAAAAVSGSQKRPTSVPLNQAPGPTPDTKSLPEVNRARMHLILGVSIQFLCSPRPEEPIEHVTACLQALRTLLDSPCARIHIAEDQLVGVELLSVVHRLLLTWNPPSVQLLVTGVVQQIVRAAQDYLQEKRNTLNEEDLEKETCPTLGEGGDGGGLVPGKSLVFATMELLMFILVRHMPHLSTKMSDSPSHAGTKTPLSEESARLVAATVTILSDLPSLCSPAGCMTILPTILFLIARILKDTAIKSADNQVPPPVSAALQGIKNIVTLPLVKTDETSKQWTELIRSTLACILEYSQPDESLPTLDEVSMLTAIALFLWSASTEIIGVQSLQNGCMNRFKNALNSCDPWVQAKCYQLLLSLFQHSNRALSTPYIHSLAPIMVEKLKAVERNRPNSNIELLAVQEGIKVLETLVALGEEQNRVQLLALLVPTLISYLLDENAFASAPLASRDLHEFALQNLMRIGPLYPHAFKTVMGAAPELKIRLETAVRASQASKAKAAARQPAPTAHSAPTIKLKTSFF

Target ORF information:

RefSeq Version	XM_029050045.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus HEAT repeat containing 5B (HEATR5B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029050045.1

ORF Insert Sequence:

ATGGAGTTGG CCCATAGTTT ATTGCTAAAT GAAGAAGCTT TGGCCCAGAT TACTGAAGCA 
AAAAGGCCCG TCTTTATCTT TGAGTGGCTA CGATTTCTTG ATAAAGTTCT TGTTGCCGCC 
AACAAGACCG ATGTAAAAGA AAAACAGAAA AAGCTCGTCG AGCAGCTCAC CGGATTAATA 
AGTAGTTCAC CTGGACCGCC TACGCGGAAG CTGCTGGCTA AAAATCTAGC AGCCCTCTAC 
AGCATTGGAG ATACTTTTAC GGTGTTTCAA ACACTAGACA AGTGTAATGA TATTATCAAA 
AATAAAGATG ACACAGCAGG CTACTTGCCA ACTAAACTTG CCGCGGTGGC ATGTGTCGGA 
GCGTTTTATG AAAAAATGGG AAGGATGTTG GGAAGTGCAT TTCCAGAAAC AGTAAACAAT 
CTTTTAAAAT CTCTGAAAAG TGCTGAGTCA CAAGGTCGAA GTGAAATCTT AATGAGTTTA 
AAAAAAGTCC TGAACGGACT GGGTGGTGCA GCAGCTTCCT GTCATCGGGA TATTTACAAA 
AATGCCAGGT CTCTCCTGAC TGACAGATCT ATGGCCGTTC GATGTGCCGT CGCCAAGTGT 
CTGCTAGAAT TACAGAATGA AGCTGTATTT ATGTGGACAG CTGAATTGGA AAATGTGGCC 
ACACTCTGTT TCAAGGCTTT AGAGAGCTCA AATTATGGAG TTCGAGTTGC GGTGTCAAAA 
CTGCTAGGAA CCGTCATGGC TACAGCACTG ATGCCAAAAC AAGCCGCAGT GATGCGCCAG 
AATGTGAAGC GAGCTACCCT AGAAGAGGTC CTGGAACTCA TGGCCACAGG ATTTCTCCGT 
GGCGGATCTG GATTTTTAAA GAGTGGTGGT GAAATGTTAA AAGTAGGAGG ATCTGTCAAT 
CGTGAAGTGA GAGTTGGAGT TACGCAGGCC TACGTTGTGT TTGTTACAAC ACTGGGTGGC 
CAGTGGCTGG AGCGTAACTT TGCTACGTTC TTGTCTCATG TGCTTGATCT GGTTTCTCAC 
CCCCGGGCGA CACAAACACA CGTGGAGGCT GTATACTCTC GAAGATGTGT CTCCTTCATC 
CTTAGAGCTA CTGTGGGCAG TTTGCTAGGG GAAAAGGCCC AGATTGCAGC TGCTAAAGAA 
ATCTGCCAAG CCATTGGAAA ACAAATGAAA GCTGTGGAAA TAGTCGTTAA TGATACCAGC 
GGTGAAAACA AGTCTGGAGC AGCAGAAATA GCTGCAAGCC AGCATGTCAT GGTCTGTGCA 
CTACAGGAAC TGGGGAGCCT CGTCCAGAGC CTAAACGCTA CAGCATCTCC TCTTATTCAG 
GAGGCATCGA TAGGGCTGTT GGAGACGGTC ACTTCCGTGC TGCTTCACCC GAGTATGGCT 
GCTCGACTGG CTGCAGCGTG GTGTTTGCGC TGTGTAGCGG TGGCGTTGCC TTTCCAGTTG 
ACGCCATTTC TCGACAGATG TGCAGAAAGA CTGAACAATC TGAAGACTTC CCCCGAAGCT 
GTTAGTGGAT ATAGTTTTGC AATGGCTGCT TTACTGGGAG GAGTTCATCA GTGTCCGTTG 
GGAATCCCTC ATGCCAAAGG AAAGATGGTG GTCAGCATTG CTGAAGACCT CTTGCGTACC 
GCCGCTCAAA ATAGCAGGCT CTCCTTACAG CGCACTCAAG CTGGCTGGCT TTTACTTGGA 
GCTCTCATGA CTTTAGGTCC TTCGGTTGTT CGTTACCATC TGCCCAAGAT GCTGTTATTG 
TGGCGAAATG TCTTTCCTCG TTCCTTAAAG GAGCTGGAAG CCGAGAAGGC TCGAGGAGAC 
TCTTTTACCT GGCAAGTAAC ATTGGAAGGT CGGGCTGGAG CGCTATGTGC CATGAGGAGT 
TTTGTTGCTC ATTGTCCCGA GCTCCTAACG GAAGATGTGA TTAGAAAATT GATGACACCT 
ATAGAATGTG CCATGACTAT GATGTCACAC ATTCCATCTG TAATCAAAGC CCACGGAGCG 
CATCTGAAAG CCAGCGCTGC AATGGTCCGT TTACGGCTGT ATGACATTTT GGCTTTGTTA 
CCTCCCAAAA CTTATGAAGG GTCTTTTAAC GCACTTCTTA GAGAGCTGGT GGCAGAATTT 
ACTTTGACGG ACAACTCCGC CAACACAACT ACATCCCTCC TTAGATCTCT CTGCCACTAC 
GATGACAGCG TCCTTCTTGG TTCCTGGCTC CAGGAAACCG ATCATAAGTC AATTGAAGAT 
CAGCTGCAGC CAAACAGCGC GTCGGGAAGC GGTGCTCTGG AACACGACCC GTCTTCCATT 
TATCTGCGCA TCCCCGCGGG TGAAGCCGTG CCCGGGCCCC TGCCCCTGGG GGTCTCCGTC 
ATCGACGCCT CCGTGGCTCT TTTCGGCGTA GTGTTTCCTC ATGTCTCCTA TAAACATAGA 
TTGCAGATGT TGGATCACTT TGCGGAATGT GTCAAGCAGG CTAAGGGTGT TCGTCAACAG 
GCTGTGCAGC TTAATATTTT CACTGCAGTG CTTAGTGCAT TAAAGGGTTT AGCTGAAAAC 
AAAAGCACGT TAGGACCCGA GGAAGTGCGC AAGTCTGCTC TGACCCTAGT TATGGGCGCC 
CTCGATAACC CTAACCCAAT TTTAAGATGT GCCGCGGGAG AAGCCCTTGG ACGAATGGCT 
CAAGTGGTGG GAGAAGCAAC TTTTATTGCT AGAATGGCTC AGTATAGTTT TGACAAGTTG 
AAATCTGCTC GAGATGTTGT ATCGAGAACA GGTCATTCAT TGGCTCTTGG CTGTCTTCAC 
CGTTACGTTG GAGGAATAGG TTCAGGACAA CACCTGAAGA CCAGCGTCAG CATTTTACTG 
GCTTTGGCAC AGGATGGAAC TTCACCTGAG GTTCAGACCT GGTCTCTCCA TTCGCTCGCT 
CTGATTGTGG ATTCCAGCGG GCCAATGTAC CGTGGATATG TGGAACCGAC CTTGTCCCTA 
GTTCTTACTT TGCTGTTGAC CGTTCCACCG TCACATATAG AAGTTCATCA ATGTTTGGGT 
CGATGTCTGG GTGCTATAAT AACTACTGTT GGGCCTGAAT TGCAAGGGAA TGGGGCAACG 
ATCTCAACAA TCCGCTCTTC CTGCCTGGTG GGTTGTGCGA TCACTCAAGA CCATTCAGAT 
TCTCTAGTCC AAGCCGCTGC CATATCGTGC CTTCAGCAGC TTCATATGTT TGCCCCAAGG 
CATGTCAATC TGTCAAGCCT GGTACCTAGC CTTTGTGTTC ATTTATGCAG TTCCCATCTG 
TTACTTCGGC GTGCGGCCGT TGCCTGCCTC CGACAGCTGG CGCAGCGAGA GGCAGCTGAA 
GTCTGTGAGT ACGCCATGAG CCTGGCAAAA AACGCCGGGG ACAAAGAGAG CGGCGGGTCC 
AGAGTAGCCT CTCGGAGGGA TCTCCACTGC CGGCACCAGG GCGTCAACAT AACGGAGACC 
GGCCTCGAAG GACTGCTTTT TGGGATGCTG GACCGCGAGA CGGATCGAAA ACTCTGCTCC 
GACATCCACG ATACCCTGGG ACATATGCTT TCGTCTCTGG CAGTCGAAAA GCTCTCGCAT 
TGGTTGATGC TGTGTAAAGA TGTTCTCGCC GCTTCCGGTG ACATGAGCGC CGCGAATCCT 
TTCGGGGGAC TGAAGGATGA GGAATCCGAA AAAAAGGACG AGATGGATGA CGATACCATG 
TTCACCACCC TGGGAGAAGA CGAGAAGTCC AAACCTTCTG TAGCTCCTCG CTGGGCCACT 
CGTGTATTTG CCGCTGACTG CCTGTGTCGA ATCATCTTGC TCTGCGAGAA CGCTGACCAG 
GCCCATTTCG ATCTCGCCCT AGCCCGTTCG GCCAAACTTC GGAACCCAAA AAATGACCTC 
TTAGTGCTCC ACCTCTCCGA CCTGATCCGT ATGGCATTTA TGGCTGCCAC GGATCATAGC 
AACCAGCTGC GGATGGCTGG ACTCCAAACC CTCGAGGACA TTATCAAGAA GTTTGCATCT 
GTGCCCGAGC CAGAATTTCC AGGCCACGTA ATATTGGAGC AGTATCAAGC TAATGTGGGA 
GCTGCTTTAA GACCAGCTTT TTCACAAGAT ACTCCATCAG ATATAATAGC AAAAGCTTGC 
CAGGTCTGCA GTACCTGGAT TGGCAGCGGA GTCGTAAGCG ACCTCAATGA CCTTCGCCGA 
GTCCACAATT TGCTGGTCTC CTCCCTGGAC AAAGTACAGG CGGGAAAAGG GTCTTCAAGC 
CAACTTTATA GAGAGAGTGC CACCACCATG GAAAAACTGG CTGTCCTGAA AGCTTGGGCT 
GAGGTCTACG TCGTTGCGAT GAAAATTAAG AGGGAAGCCG AGATAAAGCC AAAAAGAGCC 
GTTAAGAGCA CGGAGGAAGA CGACGACGAT TATGGGGCTG CAGATGAGCT GCCTCCAGAC 
AGCTTAATAA CCCTGGTGCA GCCCGAGCTG CCTATACTTA GCCGCCTTTG GTTAGCGGCG 
TTAAAAGACT ACGCGCTTCT GACTCTGCCC GCTGAATTCG CCAGCCAGCT CCCGCCAGAT 
GGCGGGGCCT TTTATACTCC CGAGACCATC GATACCGCTA GGCTTCACTA TCGGAATTCT 
TGGGCTCCAA TTCTCCACGC AGCGGCCCTC TGGCTGAACA GCACGGGATT CGACACCCCG 
GAGGTCGCGG AAGAAGCGGC GGCGGCCGCC GTCTCCGGTT CTCAGAAGCG TCCTACGTCC 
GTCCCGTTAA ACCAGGCGCC TGGGCCAACT CCCGATACGA AATCTTTACC CGAAGTAAAC 
AGAGCCCGAA TGCATCTGAT TTTAGGTGTC AGTATACAGT TCCTCTGCTC CCCAAGACCC 
GAAGAACCTA TTGAACACGT TACAGCCTGT CTTCAAGCAC TCCGTACCTT ACTGGACTCT 
CCCTGTGCCA GAATCCATAT TGCAGAAGAT CAGCTCGTTG GCGTGGAGTT GCTGAGTGTG 
GTACATCGAC TCCTTTTGAC CTGGAATCCG CCGTCGGTCC AGTTGCTGGT TACCGGAGTA 
GTGCAACAGA TCGTAAGGGC GGCTCAGGAC TATTTGCAGG AAAAAAGGAA CACGCTAAAT 
GAAGAGGATT TAGAAAAAGA GACCTGCCCC ACGTTAGGGG AAGGGGGTGA CGGCGGTGGT 
CTCGTCCCGG GGAAATCTCT TGTCTTTGCT ACTATGGAAC TGCTGATGTT TATCTTGGTG 
AGGCACATGC CGCATCTCAG CACAAAAATG TCCGACTCGC CGAGTCACGC CGGCACCAAA 
ACGCCGTTGT CAGAAGAGAG CGCCCGACTG GTGGCTGCCA CGGTAACCAT CCTCTCGGAC 
CTGCCGTCGC TCTGTTCCCC TGCAGGCTGT ATGACGATAC TCCCCACGAT CCTGTTTCTC 
ATTGCGAGAA TCTTGAAGGA CACGGCAATT AAGTCGGCGG ATAATCAGGT CCCCCCTCCG 
GTCAGCGCGG CTTTACAGGG GATTAAAAAT ATCGTGACTC TCCCACTGGT GAAGACCGAC 
GAAACGAGCA AGCAGTGGAC CGAACTGATT CGCAGCACTC TCGCTTGTAT CCTGGAATAT 
TCTCAACCAG ATGAGTCTTT GCCCACTCTT GACGAAGTGA GCATGCTCAC AGCTATCGCA 
CTCTTCCTGT GGTCCGCAAG TACTGAAATA ATAGGAGTCC AGTCGTTACA GAACGGCTGC 
ATGAACAGAT TTAAGAACGC GTTGAATTCC TGCGATCCGT GGGTTCAGGC CAAATGTTAC 
CAACTTCTCC TTTCACTGTT CCAACACTCC AATCGGGCCC TCTCAACTCC GTACATCCAT 
TCATTGGCCC CGATAATGGT TGAAAAACTG AAAGCCGTGG AGAGGAACCG GCCAAACAGC 
AACATAGAAC TTTTAGCGGT CCAAGAAGGG ATCAAAGTCC TCGAAACCTT GGTTGCCCTT 
GGTGAAGAGC AGAACAGAGT GCAGCTGCTT GCCCTCTTGG TTCCCACTTT GATCTCTTAT 
CTACTGGATG AAAACGCTTT TGCTTCGGCA CCTCTGGCCT CCAGAGATCT GCACGAGTTT 
GCCCTGCAGA ATCTGATGCG TATCGGACCC CTCTACCCTC ACGCTTTCAA GACGGTAATG 
GGAGCCGCTC CCGAGTTGAA GATCCGTCTG GAAACTGCAG TGAGAGCGAG CCAGGCCAGC 
AAAGCCAAGG CAGCCGCCCG GCAGCCGGCC CCCACCGCAC ACTCTGCCCC GACGATCAAA 
CTCAAGACGA GCTTTTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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HEATR5B cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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