NOS3 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOS3
Entrez Gene ID	100009498
Full Name	nitric oxide synthase 3
Synonyms	eNOS
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017345497.1	XP_017200986.1	nitric oxide synthase, endothelial isoform X1
NM_001082733.1	NP_001076202.1	nitric oxide synthase, endothelial

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Nitric oxide synthase and changes in oxidative stress levels in embryonic kidney observed in a rabbit model of intrauterine growth restriction.
Figueroa H, Cifuentes J, Lozano M, Alvarado C, Cabezas C, Eixarch E, Fern?ndez E, Contreras L, Illanes SE, Hern?ndez-Andrade E, Gratac?s E, Irarrazabal CE
Prenatal diagnosis36(7)628-35(2016 Jul)

Slit2 modifies VEGF-induced angiogenic responses in rabbit skeletal muscle via reduced eNOS activity.
Nieminen T, Toivanen PI, Laakkonen JP, Heikura T, Kaikkonen MU, Airenne KJ, Yl?-Herttuala S
Cardiovascular research107(2)267-76(2015 Jul)

Expression and localization of NO synthase isoenzymes (iNOS and eNOS) in development of the rabbit placenta.
Khan H, Kusakabe KT, Wakitani S, Hiyama M, Takeshita A, Kiso Y
The Journal of reproduction and development58(2)231-6(2012)

Decreased tetrahydrobiopterin and disrupted association of Hsp90 with eNOS by hyperglycemia impair myocardial ischemic preconditioning.
Vladic N, Ge ZD, Leucker T, Brzezinska AK, Du JH, Shi Y, Warltier DC, Pratt PF Jr, Kersten JR
American journal of physiology. Heart and circulatory physiology301(5)H2130-9(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kidneys from circulation-restricted fetuses showed endothelial nitric oxide synthase phosphorylation inhibition.
Title: Nitric oxide synthase and changes in oxidative stress levels in embryonic kidney observed in a rabbit model of intrauterine growth restriction.

VEGFR2 activation was not affected by Slit2, but eNOS phosphorylation was diminished
Title: Slit2 modifies VEGF-induced angiogenic responses in rabbit skeletal muscle via reduced eNOS activity.

Pulmonary ischaemia-reperfusion up-regulates inducible nitric oxide synthesis and/activity, which coincides with reduced endothelial nitric oxide synthase activity.
Title: Interplay of endothelial and inducible nitric oxide synthases modulates the vascular response to ischaemia-reperfusion in the rabbit lung.

Data suggest distinct localizations of eNOS at the spiral arteries/arterial sinuses and iNOS along the radial arteries in the developing placenta.
Title: Expression and localization of NO synthase isoenzymes (iNOS and eNOS) in development of the rabbit placenta.

eNOS dysregulation may be a central mechanism of impaired cardioprotection during hyperglycemia.
Title: Decreased tetrahydrobiopterin and disrupted association of Hsp90 with eNOS by hyperglycemia impair myocardial ischemic preconditioning.

The following NOS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb35662	XM_017345497.1 Latest version!	Oryctolagus cuniculus nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OOb30874	NM_001082733.1 Latest version!	Oryctolagus cuniculus nitric oxide synthase 3 (NOS3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOS3 ( XM_017345497.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017200986.1 Oryctolagus cuniculus nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOb35662
Clone ID Related Accession (Same CDS sequence)	XM_017345497.1
Accession Version	XM_017345497.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-22
Organism	Oryctolagus cuniculus(rabbit)
Product	nitric oxide synthase, endothelial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013681.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCAC CCTGTGGCCT GGGGCTGGGG 
CTGGGCCTCG GGCTGTGCGG CAAGCAGGGC CCAGCCTCCC CAGCACCAGA GCCCAGCCGG 
GCGCCCGCAT CCCCACCCCC AACCCCTCCA GCGCCAGACC ACAGCCCCAA ACCTAGCTCC 
AACAGTCCTC CGCTAACTCG GCCCCCGGAG GGGCCGAAGT TCCCTCGAGT GAAGAACTGG 
GAGGTGGGCA GCATCACCTA CGACACCCTC AGTGCCCAGG CACAGCAGGA CGGGCCTTGC 
ACCCAAAGAC GCTGCCTGGG CTCCCTTGTG TTTCCCCGGA AACTGCAGAG CCGGCCCACC 
CAGGGCGCTC CACCCCCTGA ACAGCTGCTG GTTCAGGCCA GGGACTTCAT CAACCAGTAC 
TACAGCTCCA TCAAGAGGAG CGGCTCCCAG GCCCATGAGC AACGGCTTCA GGAAGTGGAG 
GCCGAGGTGG CAGCCACAGG CACCTACCAG CTTCGGGAAA GTGAACTGGT CTTTGGGGCC 
AAGCAGGCCT GGCGCAACGC TCCCCGCTGT GTGGGCCGGA TCCAGTGGGG GAAGCTGCAG 
GTCTTCGATG CCCGGGACTG CAGGTCTGCA CAGGAAATGT TCACCTACAT CTGCAACCAC 
ATCAAGTATG CCACCAACCG GGGCAACCTT CGTTCAGCCA TCACAGTGTT CCCCCAGCGC 
GGCCCGGGCC GCGGAGACTT CCGGATCTGG AACAGCCAGC TGGTGCGCTA TGCGGGCTAC 
AGACAGCAGG ACGGCTCTGT GCGGGGGGAC CCAGCCAACG TGGAGATCAC CGAGCTCTGC 
ATCCAGCATG GCTGGACCCC AGGAAACGGG CGCTTCGATG TGCTGCCCCT GCTGCTTCAG 
GTCCCGGACG AGCCCCCAGA ACTCTTCACT CTGCCCCCTG AGTTGGTCCT GGAGGTGTCC 
CTGGAGCACC CCACGCTGGA GTGGTTCGCG GCCCTGGGTC TTCGCTGGTA TGCCCTCCCG 
GCAGTGTCCA ACATGCTGCT GGAAATCGGG GGGCTAGAGT TCCCTGCAGC CCCTTTCAGC 
GGCTGGTACA TGAGCACGGA GATCGGCACA CGGAACCTGT GTGACCCTCA CCGCTACAAC 
ATCCTGGAGG ATGTGGCCGT CTGCATGGAC TTGGATACCC GGACAACGTC GTCGCTGTGG 
AAAGACAAGG CGGCGGTGGA GATTAACTTG GCTGTGCTGC ACAGTTACCA GCTCGCCAAA 
GTGACCATTG TGGATCACCA CGCTGCCACA GCGTCCTTCA TGAAGCACCT GGAGAATGAG 
CAGAAGGCCA GAGGGGGCTG CCCTGCTGAC TGGGCCTGGA TCGTGCCCCC CATCTCGGGC 
AGCCTCACTC CTGTCTTCCA TCAAGAGATG GTCAACTATT TCCTGTCCCC TGCCTTCCGC 
TACCAGCCAG ACCCCTGGAA GGGGAGTGGA GCCAAGGGCA CTGGCATCAC CAGGAAGAAG 
ACCTTTAAGG AAGTGGCCAA TGCAGTGAAG ATCTCTGCCT CACTCATGGG CACAGTGATG 
GCAAAGAGAG TGAAGGCCAC AATCCTGTAC GGCTCCGAGA CTGGCCGGGC CCAGAGCTAC 
GCGCAGCAGC TGGGGAGGCT CTTCCGGAAG GCGTTCGACC CCCGGGTTCT GTGCATGGAC 
GAGTATGATG TGGTGTCCCT CGAACACGAG ACGCTGGTGT TGGTGGTGAC CAGCACATTT 
GGGAATGGAG ATCCACCAGA GAATGGAGAG GAAGCCAGCA CATCGGTCTC CACATCTGTC 
CAGCGTCTGG TGGCAGCCTT CTTGCTGCAC CACGACATGA CAGAGGGCAT CACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017200986.1
CDS	70..1929
Translation	MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSRAPASPPPTPPAPDHSPKPSSNSPPLTRPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTQRRCLGSLVFPRKLQSRPTQGAPPPEQLLVQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRGPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQVPDEPPELFTLPPELVLEVSLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSGAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGEEASTSVSTSVQRLVAAFLLHHDMTEGITQ

Target ORF information:

RefSeq Version	XM_017345497.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus nitric oxide synthase 3 (NOS3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017345497.1

ORF Insert Sequence:

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCAC CCTGTGGCCT GGGGCTGGGG 
CTGGGCCTCG GGCTGTGCGG CAAGCAGGGC CCAGCCTCCC CAGCACCAGA GCCCAGCCGG 
GCGCCCGCAT CCCCACCCCC AACCCCTCCA GCGCCAGACC ACAGCCCCAA ACCTAGCTCC 
AACAGTCCTC CGCTAACTCG GCCCCCGGAG GGGCCGAAGT TCCCTCGAGT GAAGAACTGG 
GAGGTGGGCA GCATCACCTA CGACACCCTC AGTGCCCAGG CACAGCAGGA CGGGCCTTGC 
ACCCAAAGAC GCTGCCTGGG CTCCCTTGTG TTTCCCCGGA AACTGCAGAG CCGGCCCACC 
CAGGGCGCTC CACCCCCTGA ACAGCTGCTG GTTCAGGCCA GGGACTTCAT CAACCAGTAC 
TACAGCTCCA TCAAGAGGAG CGGCTCCCAG GCCCATGAGC AACGGCTTCA GGAAGTGGAG 
GCCGAGGTGG CAGCCACAGG CACCTACCAG CTTCGGGAAA GTGAACTGGT CTTTGGGGCC 
AAGCAGGCCT GGCGCAACGC TCCCCGCTGT GTGGGCCGGA TCCAGTGGGG GAAGCTGCAG 
GTCTTCGATG CCCGGGACTG CAGGTCTGCA CAGGAAATGT TCACCTACAT CTGCAACCAC 
ATCAAGTATG CCACCAACCG GGGCAACCTT CGTTCAGCCA TCACAGTGTT CCCCCAGCGC 
GGCCCGGGCC GCGGAGACTT CCGGATCTGG AACAGCCAGC TGGTGCGCTA TGCGGGCTAC 
AGACAGCAGG ACGGCTCTGT GCGGGGGGAC CCAGCCAACG TGGAGATCAC CGAGCTCTGC 
ATCCAGCATG GCTGGACCCC AGGAAACGGG CGCTTCGATG TGCTGCCCCT GCTGCTTCAG 
GTCCCGGACG AGCCCCCAGA ACTCTTCACT CTGCCCCCTG AGTTGGTCCT GGAGGTGTCC 
CTGGAGCACC CCACGCTGGA GTGGTTCGCG GCCCTGGGTC TTCGCTGGTA TGCCCTCCCG 
GCAGTGTCCA ACATGCTGCT GGAAATCGGG GGGCTAGAGT TCCCTGCAGC CCCTTTCAGC 
GGCTGGTACA TGAGCACGGA GATCGGCACA CGGAACCTGT GTGACCCTCA CCGCTACAAC 
ATCCTGGAGG ATGTGGCCGT CTGCATGGAC TTGGATACCC GGACAACGTC GTCGCTGTGG 
AAAGACAAGG CGGCGGTGGA GATTAACTTG GCTGTGCTGC ACAGTTACCA GCTCGCCAAA 
GTGACCATTG TGGATCACCA CGCTGCCACA GCGTCCTTCA TGAAGCACCT GGAGAATGAG 
CAGAAGGCCA GAGGGGGCTG CCCTGCTGAC TGGGCCTGGA TCGTGCCCCC CATCTCGGGC 
AGCCTCACTC CTGTCTTCCA TCAAGAGATG GTCAACTATT TCCTGTCCCC TGCCTTCCGC 
TACCAGCCAG ACCCCTGGAA GGGGAGTGGA GCCAAGGGCA CTGGCATCAC CAGGAAGAAG 
ACCTTTAAGG AAGTGGCCAA TGCAGTGAAG ATCTCTGCCT CACTCATGGG CACAGTGATG 
GCAAAGAGAG TGAAGGCCAC AATCCTGTAC GGCTCCGAGA CTGGCCGGGC CCAGAGCTAC 
GCGCAGCAGC TGGGGAGGCT CTTCCGGAAG GCGTTCGACC CCCGGGTTCT GTGCATGGAC 
GAGTATGATG TGGTGTCCCT CGAACACGAG ACGCTGGTGT TGGTGGTGAC CAGCACATTT 
GGGAATGGAG ATCCACCAGA GAATGGAGAG GAAGCCAGCA CATCGGTCTC CACATCTGTC 
CAGCGTCTGG TGGCAGCCTT CTTGCTGCAC CACGACATGA CAGAGGGCAT CACACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOS3 ( NM_001082733.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001076202.1 Oryctolagus cuniculus nitric oxide synthase 3 (NOS3), mRNA.

Price & Availability↑

CloneID	OOb30874
Clone ID Related Accession (Same CDS sequence)	NM_001082733.1
Accession Version	NM_001082733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-15
Organism	Oryctolagus cuniculus(rabbit)
Product	nitric oxide synthase, endothelial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY179960.1. ##Evidence-Data-START## Transcript exon combination :: AY179960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3321803, SAMEA3321805 [ECO:0000348] ##Evidence-Data-END##

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCAC CCTGTGGCCT GGGGCTGGGG 
CTGGGCCTCG GGCTGTGCGG CAAGCAGGGC CCAGCCTCCC CAGCACCAGA GCCCAGCCGG 
GCGCCCGCAT CCCCACCCCC AACCCCTCCA GCGCCAGACC ACAGCCCCAA ACCTAGCTCC 
AACAGTCCTC CGCTAACTCG GCCCCCGGAG GGGCCGAAGT TCCCTCGAGT GAAGAACTGG 
GAGGTGGGCA GCATCACCTA CGACACCCTC AGTGCCCAGG CACAGCAGGA CGGGCCTTGC 
ACCCAAAGAC GCTGCCTGGG CTCCCTTGTG TTTCCCCGGA AACTGCAGAG CCGGCCCACC 
CAGGGCGCTC CACCCCCTGA ACAGCTGCTG GTTCAGGCCA GGGACTTCAT CAACCAGTAC 
TACAGCTCCA TCAAGAGGAG CGGCTCCCAG GCCCATGAGC AACGGCTTCA GGAAGTGGAG 
GCCGAGGTGG CAGCCACAGG CACCTACCAG CTTCGGGAAA ATGAACTGGT CTTTGGGGCC 
AAGCAGGCCT GGCGCAACGC TCCCCGCTGT GTGGGCCGGA TCCAGTGGGG GAAGCTGCAG 
GTCTTCGATG CCCGGGACTG CAGGTCTGCA CAGGAAATGT TCACCTACAT CTGCAACCAC 
ATCAAGTATG CCACCAACCG GGGCAACCTT CGTTCAGCCA TCACAGTGTT CCCCCAGCGC 
GGCCCGGGCC GCGGAGACTT CCGGATCTGG AACAGCCAGC TGGTGCGCTA TGCGGGCTAC 
AGACAGCAGG ACGGCTCTGT GCGGGGGGAC CCAGCCAACG TGGAGATCAC CGAGCTCTGC 
ATCCAGCACG GCTGGACCCC AGGAAACGGG CGCTTCGATG TGCTGCCCCT GCTGCTTCAG 
GTCCCGGACG AGCCCCCAGA ACTCTTCACT CTGCCCCCTG AGTTGGTCCT GGAGGTGTCC 
CTGGAGCACC CCACGCTGGA GTGGTTCGCG GCCCTGGGTC TTCGCTGGTA TGCCCTCCCG 
GCAGTGTCCA ACATGCTGCT GGAAATCGGG GGGCTAGAGT TCCCTGCAGC CCCTTTCAGC 
GGCTGGTACA TGAGCACGGA GATCGGCACA CGGAACCTGT GTGACCCTCA CCGCTACAAC 
ATCCTGGAGG ATGTGGCCGT CTGCATGGAC TTGGATACCC GGACAACGTC GTCCCTGTGG 
AAAGACAAGG CGGCGGTGGA GATTAACTTG GCTGTGCTGC ACAGTTACCA GCTCGCCAAA 
GTGACCATTG TGGATCACCA CGCTGCCACA GCGTCCTTCA TGAAGCACCT GGAGAATGAG 
CAGAAGGCCA GAGGGGGCTG CCCTGCTGAC TGGGCCTGGA TCGTGCCCCC CATCTCGGGC 
AGCCTCACTC CTGTCTTCCA TCAAGAGATG GTCAACTATT TCCTGTCCCC TGCCTTCCGC 
TACCAGCCAG ACCCCTGGAA GGGGAGTGGA GCCAAGGGCA CTGGCATCAC CAGGAAGAAG 
ACCTTTAAGG AAGTGGCCAA TGCAGTGAAG ATCTCTGCCT CACTCATGGG CACAGTGATG 
GCAAAGAGAG TGAAGGCCAC AATCCTGTAC GGCTCCGAGA CTGGCCGGGC CCAGAGCTAC 
GCGCAGCAGC TGGGGAGGCT CTTCCGGAAG GCGTTCGACC CCCGGGTTCT GTGCATGGAC 
GAGTATGATG TGGTGTCCCT CGAACACGAG ACGCTGGTGT TGGTGGTGAC CAGCACATTT 
GGGAATGGAG ATCCACCAGA GAACGGAGAG AGTTTTGCAG CAGCCCTGAT GGAGATGTCC 
GGCCCCTACA ACAGCTCTCC GCGGCCAGAG CAGCACAAGA GTTACAAAAT CCGCTTCAAC 
AGCGTCTCCT GCTCAGACCC GCTGGTGTCT TCTTGGCGGC GGAAGAGGAA GGAGTCCAGT 
AACACAGACA GTGCAGGGGC TCTAGGCACC CTCAGGTTCT GTGTGTTCGG CCTGGGCTCC 
CGGGCATACC CCCACTTCTG TGCCTTTGCT CGTGCGGTGG ACACGCGGCT GGAAGAGCTG 
GGCGGGGAGC GGCTGCTGCA GCTGGGCCAG GGCGATGAGC TATGCGGCCA GGAGGAGGCC 
TTCCGCGGCT GGGCCCAGGC TGCCTTCCAG GCCTCCTGTG AGACTTTCTG CGTGGGAGAG 
GATGCCAAGG CTGCTGCCCG GGACATATTT AGCCCCAAAC GGAGCTGGAA GCGCCAGAGG 
TACCGGCTGA GTACCCAGAC CGAGGGCCTA CAGTTGCTGC CAGGTCTGAT CCACGTGCAC 
AGGCGGAAGA TGTTCCAGGC CACAATCCTC TCAGTAGAAA ACCTGCAAAG CAGCAAATCC 
ACCCGGGCCA CAATCCTGGT GCGTCTGGAT ACTGGTGGCC AGGAAGGGCT ACAGTACCAG 
CCAGGGGACC ACATAGGAGT CTGCCCACCC AACCGGCCTG GCCTCGTGGA GGCGCTGCTG 
AGCCGTGTGG AGGACCCGCC GCCACCCACG GAGCCTGTGG CAGTGGAGCA GCTGGAGAAA 
GGCAGCCCTG GTGGCCCTCC CCCCAGCTGG GTGCGGGACC CCCGGCTGCC CCCGTGCTCG 
CTGCGCCAGG CTCTCACCTT CTTCCTGGAC ATCACCTCTC CATCCAGTCC TCGACTCCTT 
CGACTGCTCA GCACCTTGGC TGAAGAGCCC AGCGAACAGC AGGAGTTGGA GGCCCTCAGC 
CAGGACCCCC GGCGCTACGA GGAGTGGAAG TGGTTCCGCT GCCCCACGCT GCTGGAGGTG 
CTGGAGCAAT TCCCGTCCGT GGCACTGCCT GCTCCCCTGC TCCTCACCCA GCTGCCCCTG 
CTGCAGCCCC GGTACTACTC AGTCAGCTCG GCACCCAGCA TCTACCCAGG AGAGATCCAC 
CTGACTGTCG CTGTGCTGGC CTACAGGACC CAGGATGGGC TGGGCCCCCT GCACTATGGA 
GTCTGCTCCA CGTGGCTGAG CCAACTCAAG GCTGGAGACC CCGTGCCCTG CTTCATCAGG 
GGGGCTCCCT CCTTCCGGCT GCCACCTGAT CCCAGCTTGC CCTGCATCCT GGTGGGCCCC 
GGCACCGGCA TCGCCCCCTT CCGGGGGTTC TGGCAGCAGC GCCTGCATGA CATTGAGAGC 
AAAGGGCTGC AGCCCGCGCC CCTGACTCTG GTGTTCGGCT GCCGATGCTC CCAGCTGGAT 
CACCTGTACC GCGACGAGGT GCAGGACGCC CAGCAGCGCG GAGTGTTTGG CTGCGTCCTC 
ACCGCCTTCT CCAGGGAACC CGACAGCCCC AAGACCTACG TGCAGGACAT CCTGAGGACC 
GAGCTGGCGT CTGAGGTGCA CCGCGTGCTG TGCCTCGAAC GCGGCCACAT GTATGTCTGC 
GGAGATGTCA CCATGGCAAC CAGCGTCCTG CAGACGGTGC AGCGAATCCT GGCCACCGAG 
GGCGACATGG AGCTGGACGA GGCCGGTGAC GTCATCGGCG TGCTGCGGGA TCAGCAACGC 
TACCACGAGG ACATTTTCGG GCTCACGCTG CGCACCCAGG AGGTGACAAG CCGCATACGC 
ACCCAGAGTT TCTCCTTGCA GGAGCGGCAG CTGCGGGGCG CGGTGCCCTG GGCGTTCGAC 
CTACCCGGCT CCGACACCCC CGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076202.1
CDS	1..3630
Translation	MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSRAPASPPPTPPAPDHSPKPSSNSPPLTRPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTQRRCLGSLVFPRKLQSRPTQGAPPPEQLLVQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRENELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRGPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQVPDEPPELFTLPPELVLEVSLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSGAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQASCETFCVGEDAKAAARDIFSPKRSWKRQRYRLSTQTEGLQLLPGLIHVHRRKMFQATILSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPPTEPVAVEQLEKGSPGGPPPSWVRDPRLPPCSLRQALTFFLDITSPSSPRLLRLLSTLAEEPSEQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSIYPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKAGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQQRLHDIESKGLQPAPLTLVFGCRCSQLDHLYRDEVQDAQQRGVFGCVLTAFSREPDSPKTYVQDILRTELASEVHRVLCLERGHMYVCGDVTMATSVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDLPGSDTPGP

Target ORF information:

RefSeq Version	NM_001082733.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus nitric oxide synthase 3 (NOS3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082733.1

ORF Insert Sequence:

ATGGGCAACT TGAAGAGTGT GGGCCAGGAG CCCGGGCCAC CCTGTGGCCT GGGGCTGGGG 
CTGGGCCTCG GGCTGTGCGG CAAGCAGGGC CCAGCCTCCC CAGCACCAGA GCCCAGCCGG 
GCGCCCGCAT CCCCACCCCC AACCCCTCCA GCGCCAGACC ACAGCCCCAA ACCTAGCTCC 
AACAGTCCTC CGCTAACTCG GCCCCCGGAG GGGCCGAAGT TCCCTCGAGT GAAGAACTGG 
GAGGTGGGCA GCATCACCTA CGACACCCTC AGTGCCCAGG CACAGCAGGA CGGGCCTTGC 
ACCCAAAGAC GCTGCCTGGG CTCCCTTGTG TTTCCCCGGA AACTGCAGAG CCGGCCCACC 
CAGGGCGCTC CACCCCCTGA ACAGCTGCTG GTTCAGGCCA GGGACTTCAT CAACCAGTAC 
TACAGCTCCA TCAAGAGGAG CGGCTCCCAG GCCCATGAGC AACGGCTTCA GGAAGTGGAG 
GCCGAGGTGG CAGCCACAGG CACCTACCAG CTTCGGGAAA ATGAACTGGT CTTTGGGGCC 
AAGCAGGCCT GGCGCAACGC TCCCCGCTGT GTGGGCCGGA TCCAGTGGGG GAAGCTGCAG 
GTCTTCGATG CCCGGGACTG CAGGTCTGCA CAGGAAATGT TCACCTACAT CTGCAACCAC 
ATCAAGTATG CCACCAACCG GGGCAACCTT CGTTCAGCCA TCACAGTGTT CCCCCAGCGC 
GGCCCGGGCC GCGGAGACTT CCGGATCTGG AACAGCCAGC TGGTGCGCTA TGCGGGCTAC 
AGACAGCAGG ACGGCTCTGT GCGGGGGGAC CCAGCCAACG TGGAGATCAC CGAGCTCTGC 
ATCCAGCACG GCTGGACCCC AGGAAACGGG CGCTTCGATG TGCTGCCCCT GCTGCTTCAG 
GTCCCGGACG AGCCCCCAGA ACTCTTCACT CTGCCCCCTG AGTTGGTCCT GGAGGTGTCC 
CTGGAGCACC CCACGCTGGA GTGGTTCGCG GCCCTGGGTC TTCGCTGGTA TGCCCTCCCG 
GCAGTGTCCA ACATGCTGCT GGAAATCGGG GGGCTAGAGT TCCCTGCAGC CCCTTTCAGC 
GGCTGGTACA TGAGCACGGA GATCGGCACA CGGAACCTGT GTGACCCTCA CCGCTACAAC 
ATCCTGGAGG ATGTGGCCGT CTGCATGGAC TTGGATACCC GGACAACGTC GTCCCTGTGG 
AAAGACAAGG CGGCGGTGGA GATTAACTTG GCTGTGCTGC ACAGTTACCA GCTCGCCAAA 
GTGACCATTG TGGATCACCA CGCTGCCACA GCGTCCTTCA TGAAGCACCT GGAGAATGAG 
CAGAAGGCCA GAGGGGGCTG CCCTGCTGAC TGGGCCTGGA TCGTGCCCCC CATCTCGGGC 
AGCCTCACTC CTGTCTTCCA TCAAGAGATG GTCAACTATT TCCTGTCCCC TGCCTTCCGC 
TACCAGCCAG ACCCCTGGAA GGGGAGTGGA GCCAAGGGCA CTGGCATCAC CAGGAAGAAG 
ACCTTTAAGG AAGTGGCCAA TGCAGTGAAG ATCTCTGCCT CACTCATGGG CACAGTGATG 
GCAAAGAGAG TGAAGGCCAC AATCCTGTAC GGCTCCGAGA CTGGCCGGGC CCAGAGCTAC 
GCGCAGCAGC TGGGGAGGCT CTTCCGGAAG GCGTTCGACC CCCGGGTTCT GTGCATGGAC 
GAGTATGATG TGGTGTCCCT CGAACACGAG ACGCTGGTGT TGGTGGTGAC CAGCACATTT 
GGGAATGGAG ATCCACCAGA GAACGGAGAG AGTTTTGCAG CAGCCCTGAT GGAGATGTCC 
GGCCCCTACA ACAGCTCTCC GCGGCCAGAG CAGCACAAGA GTTACAAAAT CCGCTTCAAC 
AGCGTCTCCT GCTCAGACCC GCTGGTGTCT TCTTGGCGGC GGAAGAGGAA GGAGTCCAGT 
AACACAGACA GTGCAGGGGC TCTAGGCACC CTCAGGTTCT GTGTGTTCGG CCTGGGCTCC 
CGGGCATACC CCCACTTCTG TGCCTTTGCT CGTGCGGTGG ACACGCGGCT GGAAGAGCTG 
GGCGGGGAGC GGCTGCTGCA GCTGGGCCAG GGCGATGAGC TATGCGGCCA GGAGGAGGCC 
TTCCGCGGCT GGGCCCAGGC TGCCTTCCAG GCCTCCTGTG AGACTTTCTG CGTGGGAGAG 
GATGCCAAGG CTGCTGCCCG GGACATATTT AGCCCCAAAC GGAGCTGGAA GCGCCAGAGG 
TACCGGCTGA GTACCCAGAC CGAGGGCCTA CAGTTGCTGC CAGGTCTGAT CCACGTGCAC 
AGGCGGAAGA TGTTCCAGGC CACAATCCTC TCAGTAGAAA ACCTGCAAAG CAGCAAATCC 
ACCCGGGCCA CAATCCTGGT GCGTCTGGAT ACTGGTGGCC AGGAAGGGCT ACAGTACCAG 
CCAGGGGACC ACATAGGAGT CTGCCCACCC AACCGGCCTG GCCTCGTGGA GGCGCTGCTG 
AGCCGTGTGG AGGACCCGCC GCCACCCACG GAGCCTGTGG CAGTGGAGCA GCTGGAGAAA 
GGCAGCCCTG GTGGCCCTCC CCCCAGCTGG GTGCGGGACC CCCGGCTGCC CCCGTGCTCG 
CTGCGCCAGG CTCTCACCTT CTTCCTGGAC ATCACCTCTC CATCCAGTCC TCGACTCCTT 
CGACTGCTCA GCACCTTGGC TGAAGAGCCC AGCGAACAGC AGGAGTTGGA GGCCCTCAGC 
CAGGACCCCC GGCGCTACGA GGAGTGGAAG TGGTTCCGCT GCCCCACGCT GCTGGAGGTG 
CTGGAGCAAT TCCCGTCCGT GGCACTGCCT GCTCCCCTGC TCCTCACCCA GCTGCCCCTG 
CTGCAGCCCC GGTACTACTC AGTCAGCTCG GCACCCAGCA TCTACCCAGG AGAGATCCAC 
CTGACTGTCG CTGTGCTGGC CTACAGGACC CAGGATGGGC TGGGCCCCCT GCACTATGGA 
GTCTGCTCCA CGTGGCTGAG CCAACTCAAG GCTGGAGACC CCGTGCCCTG CTTCATCAGG 
GGGGCTCCCT CCTTCCGGCT GCCACCTGAT CCCAGCTTGC CCTGCATCCT GGTGGGCCCC 
GGCACCGGCA TCGCCCCCTT CCGGGGGTTC TGGCAGCAGC GCCTGCATGA CATTGAGAGC 
AAAGGGCTGC AGCCCGCGCC CCTGACTCTG GTGTTCGGCT GCCGATGCTC CCAGCTGGAT 
CACCTGTACC GCGACGAGGT GCAGGACGCC CAGCAGCGCG GAGTGTTTGG CTGCGTCCTC 
ACCGCCTTCT CCAGGGAACC CGACAGCCCC AAGACCTACG TGCAGGACAT CCTGAGGACC 
GAGCTGGCGT CTGAGGTGCA CCGCGTGCTG TGCCTCGAAC GCGGCCACAT GTATGTCTGC 
GGAGATGTCA CCATGGCAAC CAGCGTCCTG CAGACGGTGC AGCGAATCCT GGCCACCGAG 
GGCGACATGG AGCTGGACGA GGCCGGTGAC GTCATCGGCG TGCTGCGGGA TCAGCAACGC 
TACCACGAGG ACATTTTCGG GCTCACGCTG CGCACCCAGG AGGTGACAAG CCGCATACGC 
ACCCAGAGTT TCTCCTTGCA GGAGCGGCAG CTGCGGGGCG CGGTGCCCTG GGCGTTCGAC 
CTACCCGGCT CCGACACCCC CGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nitric oxide synthase and changes in oxidative stress levels in embryonic kidney observed in a rabbit model of intrauterine growth restriction.
Prenatal diagnosis36(7)628-35(2016 Jul)
Figueroa H,Cifuentes J,Lozano M,Alvarado C,Cabezas C,Eixarch E,Fern?ndez E,Contreras L,Illanes SE,Hern?ndez-Andrade E,Gratac?s E,Irarrazabal CE

Slit2 modifies VEGF-induced angiogenic responses in rabbit skeletal muscle via reduced eNOS activity.
Cardiovascular research107(2)267-76(2015 Jul)
Nieminen T,Toivanen PI,Laakkonen JP,Heikura T,Kaikkonen MU,Airenne KJ,Yl?-Herttuala S

Expression and localization of NO synthase isoenzymes (iNOS and eNOS) in development of the rabbit placenta.
The Journal of reproduction and development58(2)231-6(2012)
Khan H,Kusakabe KT,Wakitani S,Hiyama M,Takeshita A,Kiso Y

Decreased tetrahydrobiopterin and disrupted association of Hsp90 with eNOS by hyperglycemia impair myocardial ischemic preconditioning.
American journal of physiology. Heart and circulatory physiology301(5)H2130-9(2011 Nov)
Vladic N,Ge ZD,Leucker T,Brzezinska AK,Du JH,Shi Y,Warltier DC,Pratt PF Jr,Kersten JR

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NOS3 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

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Target ORF information:

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