Cd274 cDNA ORF clone, Mus musculus(house mouse)
| Gene Symbol | Cd274 |
| Entrez Gene ID | 60533 |
| Full Name | CD274 antigen |
| Synonyms | A530045L16Rik,B7h1,Pdcd1l1,Pdcd1lg1,Pdl1 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Mus musculus(house mouse) |
| Genome | |
| Summary | The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis. [provided by RefSeq, Sep 2015]. |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| XM_030251020.1 | XP_030106880.1 | programmed cell death 1 ligand 1 isoform X1 |
| XM_030251021.1 | XP_030106881.1 | programmed cell death 1 ligand 1 isoform X2 |
| NM_021893.3 | NP_068693.1 | programmed cell death 1 ligand 1 precursor |
| Homo sapiens (human) | CD274 | NP_054862.1 |
| Canis lupus familiaris (dog) | CD274 | XP_005615991.1 |
| Bos taurus (cattle) | CD274 | NP_001156884.1 |
| Mus musculus (house mouse) | Cd274 | NP_068693.1 |
| Gallus gallus (chicken) | CD274 | XP_424811.4 |
| Pan troglodytes (chimpanzee) | CD274 | XP_001140705.1 |
| Rattus norvegicus (Norway rat) | Cd274 | NP_001178883.1 |
| Danio rerio (zebrafish) | si:ch211-241b2.5 | XP_003200855.2 |
| CD274 | NP_001077358.1 | |
| Xenopus tropicalis (tropical clawed frog) | cd274 | XP_004911464.1 |
Related articles in PubMed
Crystal structure of the complex between programmed death-1 (PD-1) and its ligand PD-L2.
Lázár-Molnár E, Yan Q, Cao E, Ramagopal U, Nathenson SG, Almo SC
Proceedings of the National Academy of Sciences of the United States of America105(30)10483-8(2008 Jul)
A link between PDL1 and T regulatory cells in fetomaternal tolerance.
Habicht A, Dada S, Jurewicz M, Fife BT, Yagita H, Azuma M, Sayegh MH, Guleria I
Journal of immunology (Baltimore, Md. : 1950)179(8)5211-9(2007 Oct)
PD-1/PD-L1, but not PD-1/PD-L2, interactions regulate the severity of experimental autoimmune encephalomyelitis.
Carter LL, Leach MW, Azoitei ML, Cui J, Pelker JW, Jussif J, Benoit S, Ireland G, Luxenberg D, Askew GR, Milarski KL, Groves C, Brown T, Carito BA, Percival K, Carreno BM, Collins M, Marusic S
Journal of neuroimmunology182(1-2)124-34(2007 Jan)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
PD-L1 and PD-L2 in dermal fibroblasts is up-regulated by activated T cells in alopecia areata-affected skin.
Title: Increased expression of PD-L1 and PD-L2 in dermal fibroblasts from alopecia areata mice.
RAS signaling can upregulate tumor cell PD-L1 expression through a mechanism involving increases in PD-L1 mRNA stability via modulation of the AU-rich element-binding protein tristetraprolin.
Title: Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA.
spleen-derived IFN-gamma induces generation of PD-L1(+)-suppressive neutrophils.
Title: Spleen-derived IFN-γ induces generation of PD-L1
Data (including data from studies in transgenic/knockout mice) suggest that T-cell expression of Mirn155 is required to limit melanoma growth; miR-155, Pdcd1, Pdcd1l1, and Ctla4 appear to regulate overlapping pathways promoting antitumor immunity. [Mirn155 = microRNA 155; Pdcd1 = programmed cell death 1 protein; Pdcd1l1 = programmed cell death 1 ligand 1 protein; Ctla4 = cytotoxic T-lymphocyte-associated protein 4]
Title: Antitumor immunity is defective in T cell-specific microRNA-155-deficient mice and is rescued by immune checkpoint blockade.
mediates an innate checkpoint to control adaptive T helper responses
Title: ILC2s regulate adaptive Th2 cell functions via PD-L1 checkpoint control.
The following Cd274 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd274 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu23190 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030251020.1 , NM_021893.3 | |
| Accession Version | NM_021893.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-27 | |
| Organism | Mus musculus(house mouse) | |
| Product | programmed cell death 1 ligand 1 precursor | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC119228.10, BY193530.1, AF317088.1 and AI645624.1. On Dec 9, 2009 this sequence version replaced NM_021893.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066841.1, AK154403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGGATAT TTGCTGGCAT TATATTCACA GCCTGCTGTC ACTTGCTACG GGCGTTTACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_068693.1 |
| CDS | 84..956 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_021893.3 |
ORF Insert Sequence:
1 | ATGAGGATAT TTGCTGGCAT TATATTCACA GCCTGCTGTC ACTTGCTACG GGCGTTTACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu23190 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030251020.1 , NM_021893.3 | |
| Accession Version | XM_030251020.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | programmed cell death 1 ligand 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGGATAT TTGCTGGCAT TATATTCACA GCCTGCTGTC ACTTGCTACG GGCGTTTACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_030106880.1 |
| CDS | 458..1330 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_030251020.1 |
ORF Insert Sequence:
1 | ATGAGGATAT TTGCTGGCAT TATATTCACA GCCTGCTGTC ACTTGCTACG GGCGTTTACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu55675 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030251021.1 | |
| Accession Version | XM_030251021.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | programmed cell death 1 ligand 1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGTGCA GATTCCCTGT AGAACGGGAG CTGGACCTGC TTGCGTTAGT GGTGTACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_030106881.1 |
| CDS | 154..915 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_030251021.1 |
ORF Insert Sequence:
1 | ATGGAGTGCA GATTCCCTGT AGAACGGGAG CTGGACCTGC TTGCGTTAGT GGTGTACTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Crystal structure of the complex between programmed death-1 (PD-1) and its ligand PD-L2. |
|
A link between PDL1 and T regulatory cells in fetomaternal tolerance. |
|
PD-1/PD-L1, but not PD-1/PD-L2, interactions regulate the severity of experimental autoimmune encephalomyelitis. |