BRCA1 gene cDNA ORF clone, Ovis aries(sheep)

mRNA	Protein	Name
XM_015098800.1	XP_014954286.1	breast cancer type 1 susceptibility protein isoform X1
XM_012186166.1	XP_012041556.1	breast cancer type 1 susceptibility protein isoform X3
XM_015109531.1	XP_014965017.1	breast cancer type 1 susceptibility protein isoform X1
XM_027974467.1	XP_027830268.1	breast cancer type 1 susceptibility protein isoform X1
XM_015109533.1	XP_014965019.1	breast cancer type 1 susceptibility protein isoform X2
XM_015098806.2	XP_014954292.2	breast cancer type 1 susceptibility protein isoform X2
XM_015098805.1	XP_014954291.1	breast cancer type 1 susceptibility protein isoform X1
XM_012186165.2	XP_012041555.1	breast cancer type 1 susceptibility protein isoform X3
XM_015109532.1	XP_014965018.1	breast cancer type 1 susceptibility protein isoform X1
XM_015098806.1	XP_014954292.1	breast cancer type 1 susceptibility protein isoform X2
XM_015098801.1	XP_014954287.1	breast cancer type 1 susceptibility protein isoform X1
XM_015098802.1	XP_014954288.1	breast cancer type 1 susceptibility protein isoform X1
XM_015098804.1	XP_014954290.1	breast cancer type 1 susceptibility protein isoform X1
XM_015109530.1	XP_014965016.1	breast cancer type 1 susceptibility protein isoform X1
XM_012152391.2	XP_012007781.1	breast cancer type 1 susceptibility protein isoform X3
XM_012152392.1	XP_012007782.1	breast cancer type 1 susceptibility protein isoform X3
XM_015098800.2	XP_014954286.2	breast cancer type 1 susceptibility protein isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSm60334	XM_015098800.2 Latest version!	Ovis aries BRCA1 DNA repair associated (BRCA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm60334	XM_027974467.1 Latest version!	Ovis aries BRCA1 DNA repair associated (BRCA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSm60335	XM_015098806.2 Latest version!	Ovis aries BRCA1 DNA repair associated (BRCA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOv27528	XM_015109532.1 Latest version!	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv27528	XM_015109530.1 Latest version!	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv27528	XM_015109531.1 Latest version!	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv27529	XM_015109533.1 Latest version!	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv27530	XM_012152392.1 Latest version!	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OOv27530	XM_012152391.2 Latest version!	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm10053	XM_015098805.1 Latest version!	Ovis aries breast cancer 1 (BRCA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm10053	XM_015098804.1 Latest version!	Ovis aries breast cancer 1 (BRCA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm10053	XM_015098802.1 Latest version!	Ovis aries breast cancer 1 (BRCA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm10053	XM_015098800.1 Latest version!	Ovis aries breast cancer 1 (BRCA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm10053	XM_015098801.1 Latest version!	Ovis aries breast cancer 1 (BRCA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm29450	XM_012186165.2 Latest version!	Ovis aries breast cancer 1 (BRCA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm29450	XM_012186166.1 Latest version!	Ovis aries breast cancer 1 (BRCA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OSm51575	XM_015098806.1 Latest version!	Ovis aries breast cancer 1 (BRCA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

BRCA1 ( XM_027974467.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_027830268.1 Ovis aries BRCA1 DNA repair associated (BRCA1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSm60334
Clone ID Related Accession (Same CDS sequence)	XM_027974467.1 , XM_015098800.2
Accession Version	XM_027974467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGT GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGGA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCATTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGAATTCAG AGGGAGCCCT GGCCCTTTCA CCCATACACA TTTGGCTCAG 
GGTCACCAAA GAGGGGCTGG GAAACTAGAG TCAGAAGAGA CTGTGTCTAG TGAGGATGAA 
GAGCTTCCCT GCTTCCAACA ATTACTGTTT GGTAAAGTAA CCGATACACT GTCTCCATCT 
ACTGGATGGA ACACTATTGC CACTGAGGGT CTGTCTAAGG AAACAGAGGA GAACCTAGAA 
TCATTGAAGA GTGGCTTAAA TGACTGCAGT GGCCAGGGAA CATCAGCCAA GGTATCCCAG 
GAACATCACC TTAATGAGGA AGTGAGATGT TCTGGTAGCT TATTTTCCTC ACAGTACAGT 
GCAGTGGAAG ACTTGACCAC TAATACAAAC ACCCAGGATC CTTTCTTGAT GTTTGAGCGT 
CCTTCCAAAC AAGTGTATCA GTCTGAAAGC GAGGAAGTTC TGAGTGACAA GGAATTTGTT 
TCAGATGATG AAGAAAGGGA AACTGGCTTG GAAGAAGATA GTTGCCAAGA AGAGCAGAGT 
GTGGATTCAG ACTTAGGTGA AGCAGCATCC GACCATGTGA GTGAAACAAG CCTCTCTGAA 
GACTGTGTGG GGCTGTCCTC TCAGAGTGAT ATCTTAACCA CTCAGCAGAG GGATACCATG 
CAAGATAACC TTCTAAAGCT CCAGCAGGAA ATGGCCGAGC TGGAAGCTGT GTTAGAGCAG 
CACGGGAGCC AGCCTTCCCA CAGCTCTGCT TCCCTCACAG CTGATTCTCG TGGCCCAGAG 
CACCTGCTAA ATCTAGAGCA AGACACATCA GAAAGAGCAA TATTAACTTC AGAGAAAAGT 
AGAGACTGTT CTAGAAGCCA GAATCCTGAA AGCCTGTCTG CTGACAAGTT TCCAGTATCT 
CTGGATAGTT CTACCAATAA AAATAAAGAA CCAGGAATGG AAAGATCTTC CCCTTCTAAA 
TCCCAGTTGC CGGGTAATAG GTGGTATATG CACAGCTCAA GGAGTCTTCA GGACAGAAAC 
TGCCCATCTC AAAAGAAGCG CATTAATGTT GCTGACATGC AGGAGCAACA ACTGGCTAAG 
TGTGAGGCCC AAGATTTAAT GGGGTCTTAT TTGCCGAGAC AAGATCAAGA GGGAACCCCT 
TACCTGAAAT CTGGAATCAG CCTCTTCTCT CATGAACCCG AGTCTGATCC CTCTGAAGAC 
AGAGCCCCAG AGCCAGCTCA TGTTCACAGC ATGCCACCCA CAGCCTCTGC ACTGAAATTA 
TCCCAATTTC GAGTAGAAGA ATCTACCAAG AATCCAGCTG CTGCTCACAT TACTAATACT 
ACAGGGTGTA ACCTGAGGGA AGAAAGTGTG AGCAAAGAGA AGCCAGAAGT GTTATCTTCA 
ACAGAAAGAA GCAACAAAAG ACTTTCTATG GTGGCATCAG GCTTGACCCC AAAAGAACTC 
ATGCTTGTGC AGAAATTTGC CAGAAAACAT CACGTCACTT TAACTAATCT AATTACTGAA 
GAGACTACTC ATGTCATTAT GAAAACAGAT CCTGAGTTTG TGTGTGAACG GACACTGAAA 
TATTTTCTGG GAATTGCAGG AGGAAAATGG GTAGTTAGCT ATTTTTGGGT CATCCAGTCT 
ATTAAAGAAA GAAAGATGCT GGATGAGCAT GATTTTGAAG TCAGAGGAGA TGTTGTCAAT 
GGAAGAAACC ATCAAGGCCC AAAACGAGCA AGAGAATCCG GGGACAAGAA GATCTTCAAG 
GGCCTGGAAA TCTGTTGCTA TGGACCTTTT ACCAATATGC CCACAGATCA ACTAGAATGG 
ATGGTGCAGC TGTGTGGGGC TTCTGTGGTG AAGGAGCCCT CATCATTCAC CCCAGGCCAG 
GGTACTCACC CAGTTGTGGT TGTGCAGCCG GATGCCTGGA CAGAGGATGC TGGCTTCCAT 
GTGATCGGGC AGATGTGTGA AGCACCTGTG GTAACCCGAG AGTGGGTACT GGACAGTGTG 
GCCCTTTACC AGTGCCAGGA GCTGGACACC TACCTGGTCC CCCAGGTCGC CCAGAGCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027830268.1
CDS	1393..6978
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRSGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKEFRGSPGPFTHTHLAQGHQRGAGKLESEETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESGDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_027974467.1
Organism	Ovis aries(sheep)
Definition	Ovis aries BRCA1 DNA repair associated (BRCA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027974467.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGT GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGGA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCATTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGAATTCAG AGGGAGCCCT GGCCCTTTCA CCCATACACA TTTGGCTCAG 
GGTCACCAAA GAGGGGCTGG GAAACTAGAG TCAGAAGAGA CTGTGTCTAG TGAGGATGAA 
GAGCTTCCCT GCTTCCAACA ATTACTGTTT GGTAAAGTAA CCGATACACT GTCTCCATCT 
ACTGGATGGA ACACTATTGC CACTGAGGGT CTGTCTAAGG AAACAGAGGA GAACCTAGAA 
TCATTGAAGA GTGGCTTAAA TGACTGCAGT GGCCAGGGAA CATCAGCCAA GGTATCCCAG 
GAACATCACC TTAATGAGGA AGTGAGATGT TCTGGTAGCT TATTTTCCTC ACAGTACAGT 
GCAGTGGAAG ACTTGACCAC TAATACAAAC ACCCAGGATC CTTTCTTGAT GTTTGAGCGT 
CCTTCCAAAC AAGTGTATCA GTCTGAAAGC GAGGAAGTTC TGAGTGACAA GGAATTTGTT 
TCAGATGATG AAGAAAGGGA AACTGGCTTG GAAGAAGATA GTTGCCAAGA AGAGCAGAGT 
GTGGATTCAG ACTTAGGTGA AGCAGCATCC GACCATGTGA GTGAAACAAG CCTCTCTGAA 
GACTGTGTGG GGCTGTCCTC TCAGAGTGAT ATCTTAACCA CTCAGCAGAG GGATACCATG 
CAAGATAACC TTCTAAAGCT CCAGCAGGAA ATGGCCGAGC TGGAAGCTGT GTTAGAGCAG 
CACGGGAGCC AGCCTTCCCA CAGCTCTGCT TCCCTCACAG CTGATTCTCG TGGCCCAGAG 
CACCTGCTAA ATCTAGAGCA AGACACATCA GAAAGAGCAA TATTAACTTC AGAGAAAAGT 
AGAGACTGTT CTAGAAGCCA GAATCCTGAA AGCCTGTCTG CTGACAAGTT TCCAGTATCT 
CTGGATAGTT CTACCAATAA AAATAAAGAA CCAGGAATGG AAAGATCTTC CCCTTCTAAA 
TCCCAGTTGC CGGGTAATAG GTGGTATATG CACAGCTCAA GGAGTCTTCA GGACAGAAAC 
TGCCCATCTC AAAAGAAGCG CATTAATGTT GCTGACATGC AGGAGCAACA ACTGGCTAAG 
TGTGAGGCCC AAGATTTAAT GGGGTCTTAT TTGCCGAGAC AAGATCAAGA GGGAACCCCT 
TACCTGAAAT CTGGAATCAG CCTCTTCTCT CATGAACCCG AGTCTGATCC CTCTGAAGAC 
AGAGCCCCAG AGCCAGCTCA TGTTCACAGC ATGCCACCCA CAGCCTCTGC ACTGAAATTA 
TCCCAATTTC GAGTAGAAGA ATCTACCAAG AATCCAGCTG CTGCTCACAT TACTAATACT 
ACAGGGTGTA ACCTGAGGGA AGAAAGTGTG AGCAAAGAGA AGCCAGAAGT GTTATCTTCA 
ACAGAAAGAA GCAACAAAAG ACTTTCTATG GTGGCATCAG GCTTGACCCC AAAAGAACTC 
ATGCTTGTGC AGAAATTTGC CAGAAAACAT CACGTCACTT TAACTAATCT AATTACTGAA 
GAGACTACTC ATGTCATTAT GAAAACAGAT CCTGAGTTTG TGTGTGAACG GACACTGAAA 
TATTTTCTGG GAATTGCAGG AGGAAAATGG GTAGTTAGCT ATTTTTGGGT CATCCAGTCT 
ATTAAAGAAA GAAAGATGCT GGATGAGCAT GATTTTGAAG TCAGAGGAGA TGTTGTCAAT 
GGAAGAAACC ATCAAGGCCC AAAACGAGCA AGAGAATCCG GGGACAAGAA GATCTTCAAG 
GGCCTGGAAA TCTGTTGCTA TGGACCTTTT ACCAATATGC CCACAGATCA ACTAGAATGG 
ATGGTGCAGC TGTGTGGGGC TTCTGTGGTG AAGGAGCCCT CATCATTCAC CCCAGGCCAG 
GGTACTCACC CAGTTGTGGT TGTGCAGCCG GATGCCTGGA CAGAGGATGC TGGCTTCCAT 
GTGATCGGGC AGATGTGTGA AGCACCTGTG GTAACCCGAG AGTGGGTACT GGACAGTGTG 
GCCCTTTACC AGTGCCAGGA GCTGGACACC TACCTGGTCC CCCAGGTCGC CCAGAGCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015098800.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014954286.2 Ovis aries BRCA1 DNA repair associated (BRCA1), transcript variant X1, mRNA.

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CloneID	OSm60334
Clone ID Related Accession (Same CDS sequence)	XM_027974467.1 , XM_015098800.2
Accession Version	XM_015098800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015098800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGT GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGGA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCATTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGAATTCAG AGGGAGCCCT GGCCCTTTCA CCCATACACA TTTGGCTCAG 
GGTCACCAAA GAGGGGCTGG GAAACTAGAG TCAGAAGAGA CTGTGTCTAG TGAGGATGAA 
GAGCTTCCCT GCTTCCAACA ATTACTGTTT GGTAAAGTAA CCGATACACT GTCTCCATCT 
ACTGGATGGA ACACTATTGC CACTGAGGGT CTGTCTAAGG AAACAGAGGA GAACCTAGAA 
TCATTGAAGA GTGGCTTAAA TGACTGCAGT GGCCAGGGAA CATCAGCCAA GGTATCCCAG 
GAACATCACC TTAATGAGGA AGTGAGATGT TCTGGTAGCT TATTTTCCTC ACAGTACAGT 
GCAGTGGAAG ACTTGACCAC TAATACAAAC ACCCAGGATC CTTTCTTGAT GTTTGAGCGT 
CCTTCCAAAC AAGTGTATCA GTCTGAAAGC GAGGAAGTTC TGAGTGACAA GGAATTTGTT 
TCAGATGATG AAGAAAGGGA AACTGGCTTG GAAGAAGATA GTTGCCAAGA AGAGCAGAGT 
GTGGATTCAG ACTTAGGTGA AGCAGCATCC GACCATGTGA GTGAAACAAG CCTCTCTGAA 
GACTGTGTGG GGCTGTCCTC TCAGAGTGAT ATCTTAACCA CTCAGCAGAG GGATACCATG 
CAAGATAACC TTCTAAAGCT CCAGCAGGAA ATGGCCGAGC TGGAAGCTGT GTTAGAGCAG 
CACGGGAGCC AGCCTTCCCA CAGCTCTGCT TCCCTCACAG CTGATTCTCG TGGCCCAGAG 
CACCTGCTAA ATCTAGAGCA AGACACATCA GAAAGAGCAA TATTAACTTC AGAGAAAAGT 
AGAGACTGTT CTAGAAGCCA GAATCCTGAA AGCCTGTCTG CTGACAAGTT TCCAGTATCT 
CTGGATAGTT CTACCAATAA AAATAAAGAA CCAGGAATGG AAAGATCTTC CCCTTCTAAA 
TCCCAGTTGC CGGGTAATAG GTGGTATATG CACAGCTCAA GGAGTCTTCA GGACAGAAAC 
TGCCCATCTC AAAAGAAGCG CATTAATGTT GCTGACATGC AGGAGCAACA ACTGGCTAAG 
TGTGAGGCCC AAGATTTAAT GGGGTCTTAT TTGCCGAGAC AAGATCAAGA GGGAACCCCT 
TACCTGAAAT CTGGAATCAG CCTCTTCTCT CATGAACCCG AGTCTGATCC CTCTGAAGAC 
AGAGCCCCAG AGCCAGCTCA TGTTCACAGC ATGCCACCCA CAGCCTCTGC ACTGAAATTA 
TCCCAATTTC GAGTAGAAGA ATCTACCAAG AATCCAGCTG CTGCTCACAT TACTAATACT 
ACAGGGTGTA ACCTGAGGGA AGAAAGTGTG AGCAAAGAGA AGCCAGAAGT GTTATCTTCA 
ACAGAAAGAA GCAACAAAAG ACTTTCTATG GTGGCATCAG GCTTGACCCC AAAAGAACTC 
ATGCTTGTGC AGAAATTTGC CAGAAAACAT CACGTCACTT TAACTAATCT AATTACTGAA 
GAGACTACTC ATGTCATTAT GAAAACAGAT CCTGAGTTTG TGTGTGAACG GACACTGAAA 
TATTTTCTGG GAATTGCAGG AGGAAAATGG GTAGTTAGCT ATTTTTGGGT CATCCAGTCT 
ATTAAAGAAA GAAAGATGCT GGATGAGCAT GATTTTGAAG TCAGAGGAGA TGTTGTCAAT 
GGAAGAAACC ATCAAGGCCC AAAACGAGCA AGAGAATCCG GGGACAAGAA GATCTTCAAG 
GGCCTGGAAA TCTGTTGCTA TGGACCTTTT ACCAATATGC CCACAGATCA ACTAGAATGG 
ATGGTGCAGC TGTGTGGGGC TTCTGTGGTG AAGGAGCCCT CATCATTCAC CCCAGGCCAG 
GGTACTCACC CAGTTGTGGT TGTGCAGCCG GATGCCTGGA CAGAGGATGC TGGCTTCCAT 
GTGATCGGGC AGATGTGTGA AGCACCTGTG GTAACCCGAG AGTGGGTACT GGACAGTGTG 
GCCCTTTACC AGTGCCAGGA GCTGGACACC TACCTGGTCC CCCAGGTCGC CCAGAGCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014954286.2
CDS	534..6119
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRSGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKEFRGSPGPFTHTHLAQGHQRGAGKLESEETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESGDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015098800.2
Organism	Ovis aries(sheep)
Definition	Ovis aries BRCA1 DNA repair associated (BRCA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015098800.2

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGT GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGGA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCATTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGAATTCAG AGGGAGCCCT GGCCCTTTCA CCCATACACA TTTGGCTCAG 
GGTCACCAAA GAGGGGCTGG GAAACTAGAG TCAGAAGAGA CTGTGTCTAG TGAGGATGAA 
GAGCTTCCCT GCTTCCAACA ATTACTGTTT GGTAAAGTAA CCGATACACT GTCTCCATCT 
ACTGGATGGA ACACTATTGC CACTGAGGGT CTGTCTAAGG AAACAGAGGA GAACCTAGAA 
TCATTGAAGA GTGGCTTAAA TGACTGCAGT GGCCAGGGAA CATCAGCCAA GGTATCCCAG 
GAACATCACC TTAATGAGGA AGTGAGATGT TCTGGTAGCT TATTTTCCTC ACAGTACAGT 
GCAGTGGAAG ACTTGACCAC TAATACAAAC ACCCAGGATC CTTTCTTGAT GTTTGAGCGT 
CCTTCCAAAC AAGTGTATCA GTCTGAAAGC GAGGAAGTTC TGAGTGACAA GGAATTTGTT 
TCAGATGATG AAGAAAGGGA AACTGGCTTG GAAGAAGATA GTTGCCAAGA AGAGCAGAGT 
GTGGATTCAG ACTTAGGTGA AGCAGCATCC GACCATGTGA GTGAAACAAG CCTCTCTGAA 
GACTGTGTGG GGCTGTCCTC TCAGAGTGAT ATCTTAACCA CTCAGCAGAG GGATACCATG 
CAAGATAACC TTCTAAAGCT CCAGCAGGAA ATGGCCGAGC TGGAAGCTGT GTTAGAGCAG 
CACGGGAGCC AGCCTTCCCA CAGCTCTGCT TCCCTCACAG CTGATTCTCG TGGCCCAGAG 
CACCTGCTAA ATCTAGAGCA AGACACATCA GAAAGAGCAA TATTAACTTC AGAGAAAAGT 
AGAGACTGTT CTAGAAGCCA GAATCCTGAA AGCCTGTCTG CTGACAAGTT TCCAGTATCT 
CTGGATAGTT CTACCAATAA AAATAAAGAA CCAGGAATGG AAAGATCTTC CCCTTCTAAA 
TCCCAGTTGC CGGGTAATAG GTGGTATATG CACAGCTCAA GGAGTCTTCA GGACAGAAAC 
TGCCCATCTC AAAAGAAGCG CATTAATGTT GCTGACATGC AGGAGCAACA ACTGGCTAAG 
TGTGAGGCCC AAGATTTAAT GGGGTCTTAT TTGCCGAGAC AAGATCAAGA GGGAACCCCT 
TACCTGAAAT CTGGAATCAG CCTCTTCTCT CATGAACCCG AGTCTGATCC CTCTGAAGAC 
AGAGCCCCAG AGCCAGCTCA TGTTCACAGC ATGCCACCCA CAGCCTCTGC ACTGAAATTA 
TCCCAATTTC GAGTAGAAGA ATCTACCAAG AATCCAGCTG CTGCTCACAT TACTAATACT 
ACAGGGTGTA ACCTGAGGGA AGAAAGTGTG AGCAAAGAGA AGCCAGAAGT GTTATCTTCA 
ACAGAAAGAA GCAACAAAAG ACTTTCTATG GTGGCATCAG GCTTGACCCC AAAAGAACTC 
ATGCTTGTGC AGAAATTTGC CAGAAAACAT CACGTCACTT TAACTAATCT AATTACTGAA 
GAGACTACTC ATGTCATTAT GAAAACAGAT CCTGAGTTTG TGTGTGAACG GACACTGAAA 
TATTTTCTGG GAATTGCAGG AGGAAAATGG GTAGTTAGCT ATTTTTGGGT CATCCAGTCT 
ATTAAAGAAA GAAAGATGCT GGATGAGCAT GATTTTGAAG TCAGAGGAGA TGTTGTCAAT 
GGAAGAAACC ATCAAGGCCC AAAACGAGCA AGAGAATCCG GGGACAAGAA GATCTTCAAG 
GGCCTGGAAA TCTGTTGCTA TGGACCTTTT ACCAATATGC CCACAGATCA ACTAGAATGG 
ATGGTGCAGC TGTGTGGGGC TTCTGTGGTG AAGGAGCCCT CATCATTCAC CCCAGGCCAG 
GGTACTCACC CAGTTGTGGT TGTGCAGCCG GATGCCTGGA CAGAGGATGC TGGCTTCCAT 
GTGATCGGGC AGATGTGTGA AGCACCTGTG GTAACCCGAG AGTGGGTACT GGACAGTGTG 
GCCCTTTACC AGTGCCAGGA GCTGGACACC TACCTGGTCC CCCAGGTCGC CCAGAGCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015098806.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014954292.2 Ovis aries BRCA1 DNA repair associated (BRCA1), transcript variant X3, mRNA.

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CloneID	OSm60335
Clone ID Related Accession (Same CDS sequence)	XM_015098806.2
Accession Version	XM_015098806.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-05
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040262.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 6, 2019 this sequence version replaced XM_015098806.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ovis aries Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GGAAACCAGT CTTACTGTCA AACTCTCTAA CCTTGGAATT 
GTGAGATCTC TGAGGACAAA GCAGCAGACA CAATCTCAGA ATAAGTCTGT CTACATTGAA 
TTGGGATCTG ATTCTTCTGA AGACACAGTT AATAAGGCCA GTTATCTCAG TGTGGGAGAC 
CATGAATTGT TAGAAATCAC CCCTCAAGGA GCCAAGGCCA AGACCAATTT GAATCCTGCA 
AAAAAGGCTG CTTGTGAGTT TTCTGAGAAG GACATAACAA ATACTGAACA TCATCAACTC 
AGTATTAAAG ATCTGATCAC CACCCAGGAG CATGCAGCTG AGATGCATGC AGCTGAGACG 
CATCCAGAAA AGTATCAGGG TATTTCTGTT TCAGACTTTC ACGTGGAGCC ATGTGGCACA 
GATACTCATG CCAGCTCATT ACAGCATGAG AACAGCAGTT TATTGCTCAC TGAAAACAGA 
CTGAATGTAG AAAAGGCTGA ATTCTGTAAT AAAAGCAAAC AGCCTGTCTT AGTAAAGAGC 
CAACAGAGCA GATGGGCTGA AAGTAAGGGC ACATGTAAGG ATAGGCAGAT TCCCAGCACT 
GGGAAAAAGA TAGTTCTGAA TACTGATCCC CTGTATGGAA GAAAAGAACT GTGTAAGCAG 
AAACCTGCAT GCTCTGACAG TCCTGGAGAT TCCCAAGATG TTCCTTGGGT AACCCTGAAT 
AATAGCATAC AGAAAGTTAA TGACTGGTTT TCCAGAAGTG ATGAAATATT AACTTCTGAC 
GACTCGTGCG ATGGGGGGTC TGAATCAAAT AATGAAGTAG CTGGTGCAGT GGAAATTCCA 
AATAACGTTG ATGGATATTC AGGTTCTTCA GAGAAAATCG ACTTAATGGC CAGTGATCCT 
CATGGTACTT TAATACATGA AAGAGTCCAC TCCAAACCAG TAGAGAGTAA TATTGAAGAT 
AAAATATTTG GGAAAACCTA TCGGAGGAAG TCGAGTCTCC CTAACTTCAA CCACATAGCT 
GAAGATCTAA TTATAGGTGC ATTTACTGTA GAACCTCAGA AAACACAAGA GCAGCCCCTC 
ACAAATAAGC TAAAATGTAA AAGGAGTGGT ACATCAGGCC TTCAGCCTGA GGATTTTATC 
AAGAAAGGTG ATTTGACGAT TGTTCCAAAG ACTCCTGAAA AGATGATTGA GAGAACTGAC 
CAAACAGAGC AGAAACATCA CGGGATAAAT ATTACTAGTG ATGGTCATGA GAATAAAACA 
AAACGTGATG ATGTTCAGAA AGAGCAAAAC GCTAACCCAA CAGAATCATT GGAAAACGAA 
TCTGTTTTCA GAACTAAGGC TGAACCTATA AACACCAGTA TAAGCAATAT GGAACTAGAA 
TTGAATGTCC GCAGTTCAAA AGCACCTAAG AATAGGCTGA AGAGGAAATC CTCTACCAGG 
AAAATTCCTG AACTTGAACT AGTAGTCAGT AGAAATCCAA GTCCACCTAA TCATACTGAG 
CTACTAATTG ATAGCAGTTC TAGCAATGAA GAGATGAAGA AAAAGCATTC TAGCCAAATG 
CCAGTCAGGC ACAGCCAAAA GCTTCAACTC ATGGGAGATA AAGAACCTAC AGCTGGAGCC 
AAGAAGAGTA ACAAAACATA TGAACAAATA AATAAAAGAC TTGCTAGTGA TGCTTTTCCA 
GAACTAAATT TTGCAAACAC ACCTGGTTAT TTTACTAACT GTTCTAGTAA ACCTGAAGAG 
TTTGTTCATC CTAGCCTTCA AAGAGAGGAG AATCTAGGAA CAATTCAAGT GTTGAATAAT 
ACCAAAGACC CCAAAGATCT GATATTAAGA GGAGGGAAAG CTTTGCAAAT GGAAAGATCT 
GTAGAGAATA CCAATATTTC CTTGGTTCCT GATACTGATT ATAGCACTCA GGATAGTATC 
TCATTACTAG AAGCTAAAAC CCCAGAGAAG GCAAAGACTG CACCAAATCC ATGTGTGAGT 
CTGTGTGCAG CAATCGAAAA CCCCAAGGAA CTTATCCATA GGGATTTTAA AGATAACAAA 
AACAACACAG AGGGCTTTCA GGGTCTCCTG GGACATGACG TTAACTATGT CATTCAGGAG 
ACAAGAGGAG AAATGGAAGA CAGTGAACTT GATACACAGT ATTTGCAGAA TACGTTCAAG 
GTTTCAAAGC GTCAGACATT TGCTCTGTTT TCCAATCCAG GAAATCCACA AAAGGAATGT 
GCCACAGTCT TTGCCCACTC GGGGTCCTTA AAGGATCAAA GTCCAAGAGA CCCCCTCAAA 
TGCAGACAAA AAGAAGACAG TCGGGGAAAG AGTGAGTCTA AAAGCCAGCA TGTGCAGGCC 
GTTTGTACAA CAGTGCACTT TCCTGTGGCT GGTCAGAAAG ATAAGACGCC GGGTGACGAT 
GCCAAATGTA GTGCAAAAGA AGTCACTAGG GTTTGTCAGT CATTACAATT GAGAGGCCAC 
AAAACTGAAC TTGTTTTTGC TAATAAGCAA GGGGTTTCAG AAAAACCAAA TCTAACACCA 
TCACTTTTTC CCATCAAGTC ATCTGTTAAA ACCGTATGTA AGAAAAGCCC ATCAGAGAAA 
TTTGAGGAAC CTGTAATGTC ACCTGAAAGA ACATTGGGAA GTGAGAGCAT CATTCAAAGT 
GCAGTGAGCA CAATCAGCCA AAATGACATT CAAGGAAGCA CTTTTAAAGA AGTCAACTCA 
AGCAGTGTTA ATGAAGTAGG TTCCAGTACT AATGAAGTAG GCTCTAGTAT TAATGAAGTA 
GGTTCCAGTG GTGAAAACAT TCAAGCAGAA CCAGGTAGAA ACAGAGAACC TAAATTAAGA 
GCTTTACTCA GATTAGGTCT TATGCAACCT GAAGTCTATA AGCAAAGCCT TCCTGTAAGT 
AACTGTCACC ATCCTGAAAT AAAAAGGCAA GGAGAAAATG AGGACATGCT TCAGGCTGTT 
AAGGCAGATT TCTCCCCATG TCTAATTTCA GATAACCTCG AACAACCTAT GGGAAGCTGT 
CATGCTTCTC AGGTTTGTTC TGAGACACCT GACAACTTGT TAAATGACGA CGAAATAAAA 
GAAAATAGCC ACTTTGCTGA AAGTGACATT AAGGAAAGAT CTGCTGTTTT TAGTGAAAGT 
GTCCAAAAAG AATTCAGAGG GAGCCCTGGC CCTTTCACCC ATACACATTT GGCTCAGGGT 
CACCAAAGAG GGGCTGGGAA ACTAGAGTCA GAAGAGACTG TGTCTAGTGA GGATGAAGAG 
CTTCCCTGCT TCCAACAATT ACTGTTTGGT AAAGTAACCG ATACACTGTC TCCATCTACT 
GGATGGAACA CTATTGCCAC TGAGGGTCTG TCTAAGGAAA CAGAGGAGAA CCTAGAATCA 
TTGAAGAGTG GCTTAAATGA CTGCAGTGGC CAGGGAACAT CAGCCAAGGT ATCCCAGGAA 
CATCACCTTA ATGAGGAAGT GAGATGTTCT GGTAGCTTAT TTTCCTCACA GTACAGTGCA 
GTGGAAGACT TGACCACTAA TACAAACACC CAGGATCCTT TCTTGATGTT TGAGCGTCCT 
TCCAAACAAG TGTATCAGTC TGAAAGCGAG GAAGTTCTGA GTGACAAGGA ATTTGTTTCA 
GATGATGAAG AAAGGGAAAC TGGCTTGGAA GAAGATAGTT GCCAAGAAGA GCAGAGTGTG 
GATTCAGACT TAGGTGAAGC AGCATCCGAC CATGTGAGTG AAACAAGCCT CTCTGAAGAC 
TGTGTGGGGC TGTCCTCTCA GAGTGATATC TTAACCACTC AGCAGAGGGA TACCATGCAA 
GATAACCTTC TAAAGCTCCA GCAGGAAATG GCCGAGCTGG AAGCTGTGTT AGAGCAGCAC 
GGGAGCCAGC CTTCCCACAG CTCTGCTTCC CTCACAGCTG ATTCTCGTGG CCCAGAGCAC 
CTGCTAAATC TAGAGCAAGA CACATCAGAA AGAGCAATAT TAACTTCAGA GAAAAGTAGA 
GACTGTTCTA GAAGCCAGAA TCCTGAAAGC CTGTCTGCTG ACAAGTTTCC AGTATCTCTG 
GATAGTTCTA CCAATAAAAA TAAAGAACCA GGAATGGAAA GATCTTCCCC TTCTAAATCC 
CAGTTGCCGG GTAATAGGTG GTATATGCAC AGCTCAAGGA GTCTTCAGGA CAGAAACTGC 
CCATCTCAAA AGAAGCGCAT TAATGTTGCT GACATGCAGG AGCAACAACT GGCTAAGTGT 
GAGGCCCAAG ATTTAATGGG GTCTTATTTG CCGAGACAAG ATCAAGAGGG AACCCCTTAC 
CTGAAATCTG GAATCAGCCT CTTCTCTCAT GAACCCGAGT CTGATCCCTC TGAAGACAGA 
GCCCCAGAGC CAGCTCATGT TCACAGCATG CCACCCACAG CCTCTGCACT GAAATTATCC 
CAATTTCGAG TAGAAGAATC TACCAAGAAT CCAGCTGCTG CTCACATTAC TAATACTACA 
GGGTGTAACC TGAGGGAAGA AAGTGTGAGC AAAGAGAAGC CAGAAGTGTT ATCTTCAACA 
GAAAGAAGCA ACAAAAGACT TTCTATGGTG GCATCAGGCT TGACCCCAAA AGAACTCATG 
CTTGTGCAGA AATTTGCCAG AAAACATCAC GTCACTTTAA CTAATCTAAT TACTGAAGAG 
ACTACTCATG TCATTATGAA AACAGATCCT GAGTTTGTGT GTGAACGGAC ACTGAAATAT 
TTTCTGGGAA TTGCAGGAGG AAAATGGGTA GTTAGCTATT TTTGGGTCAT CCAGTCTATT 
AAAGAAAGAA AGATGCTGGA TGAGCATGAT TTTGAAGTCA GAGGAGATGT TGTCAATGGA 
AGAAACCATC AAGGCCCAAA ACGAGCAAGA GAATCCGGGG ACAAGAAGAT CTTCAAGGGC 
CTGGAAATCT GTTGCTATGG ACCTTTTACC AATATGCCCA CAGATCAACT AGAATGGATG 
GTGCAGCTGT GTGGGGCTTC TGTGGTGAAG GAGCCCTCAT CATTCACCCC AGGCCAGGGT 
ACTCACCCAG TTGTGGTTGT GCAGCCGGAT GCCTGGACAG AGGATGCTGG CTTCCATGTG 
ATCGGGCAGA TGTGTGAAGC ACCTGTGGTA ACCCGAGAGT GGGTACTGGA CAGTGTGGCC 
CTTTACCAGT GCCAGGAGCT GGACACCTAC CTGGTCCCCC AGGTCGCCCA GAGCTGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014954292.2
CDS	534..6116
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRSGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKEFRGSPGPFTHTHLAQGHQRGAGKLESEETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESGDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015098806.2
Organism	Ovis aries(sheep)
Definition	Ovis aries BRCA1 DNA repair associated (BRCA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015098806.2

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GGAAACCAGT CTTACTGTCA AACTCTCTAA CCTTGGAATT 
GTGAGATCTC TGAGGACAAA GCAGCAGACA CAATCTCAGA ATAAGTCTGT CTACATTGAA 
TTGGGATCTG ATTCTTCTGA AGACACAGTT AATAAGGCCA GTTATCTCAG TGTGGGAGAC 
CATGAATTGT TAGAAATCAC CCCTCAAGGA GCCAAGGCCA AGACCAATTT GAATCCTGCA 
AAAAAGGCTG CTTGTGAGTT TTCTGAGAAG GACATAACAA ATACTGAACA TCATCAACTC 
AGTATTAAAG ATCTGATCAC CACCCAGGAG CATGCAGCTG AGATGCATGC AGCTGAGACG 
CATCCAGAAA AGTATCAGGG TATTTCTGTT TCAGACTTTC ACGTGGAGCC ATGTGGCACA 
GATACTCATG CCAGCTCATT ACAGCATGAG AACAGCAGTT TATTGCTCAC TGAAAACAGA 
CTGAATGTAG AAAAGGCTGA ATTCTGTAAT AAAAGCAAAC AGCCTGTCTT AGTAAAGAGC 
CAACAGAGCA GATGGGCTGA AAGTAAGGGC ACATGTAAGG ATAGGCAGAT TCCCAGCACT 
GGGAAAAAGA TAGTTCTGAA TACTGATCCC CTGTATGGAA GAAAAGAACT GTGTAAGCAG 
AAACCTGCAT GCTCTGACAG TCCTGGAGAT TCCCAAGATG TTCCTTGGGT AACCCTGAAT 
AATAGCATAC AGAAAGTTAA TGACTGGTTT TCCAGAAGTG ATGAAATATT AACTTCTGAC 
GACTCGTGCG ATGGGGGGTC TGAATCAAAT AATGAAGTAG CTGGTGCAGT GGAAATTCCA 
AATAACGTTG ATGGATATTC AGGTTCTTCA GAGAAAATCG ACTTAATGGC CAGTGATCCT 
CATGGTACTT TAATACATGA AAGAGTCCAC TCCAAACCAG TAGAGAGTAA TATTGAAGAT 
AAAATATTTG GGAAAACCTA TCGGAGGAAG TCGAGTCTCC CTAACTTCAA CCACATAGCT 
GAAGATCTAA TTATAGGTGC ATTTACTGTA GAACCTCAGA AAACACAAGA GCAGCCCCTC 
ACAAATAAGC TAAAATGTAA AAGGAGTGGT ACATCAGGCC TTCAGCCTGA GGATTTTATC 
AAGAAAGGTG ATTTGACGAT TGTTCCAAAG ACTCCTGAAA AGATGATTGA GAGAACTGAC 
CAAACAGAGC AGAAACATCA CGGGATAAAT ATTACTAGTG ATGGTCATGA GAATAAAACA 
AAACGTGATG ATGTTCAGAA AGAGCAAAAC GCTAACCCAA CAGAATCATT GGAAAACGAA 
TCTGTTTTCA GAACTAAGGC TGAACCTATA AACACCAGTA TAAGCAATAT GGAACTAGAA 
TTGAATGTCC GCAGTTCAAA AGCACCTAAG AATAGGCTGA AGAGGAAATC CTCTACCAGG 
AAAATTCCTG AACTTGAACT AGTAGTCAGT AGAAATCCAA GTCCACCTAA TCATACTGAG 
CTACTAATTG ATAGCAGTTC TAGCAATGAA GAGATGAAGA AAAAGCATTC TAGCCAAATG 
CCAGTCAGGC ACAGCCAAAA GCTTCAACTC ATGGGAGATA AAGAACCTAC AGCTGGAGCC 
AAGAAGAGTA ACAAAACATA TGAACAAATA AATAAAAGAC TTGCTAGTGA TGCTTTTCCA 
GAACTAAATT TTGCAAACAC ACCTGGTTAT TTTACTAACT GTTCTAGTAA ACCTGAAGAG 
TTTGTTCATC CTAGCCTTCA AAGAGAGGAG AATCTAGGAA CAATTCAAGT GTTGAATAAT 
ACCAAAGACC CCAAAGATCT GATATTAAGA GGAGGGAAAG CTTTGCAAAT GGAAAGATCT 
GTAGAGAATA CCAATATTTC CTTGGTTCCT GATACTGATT ATAGCACTCA GGATAGTATC 
TCATTACTAG AAGCTAAAAC CCCAGAGAAG GCAAAGACTG CACCAAATCC ATGTGTGAGT 
CTGTGTGCAG CAATCGAAAA CCCCAAGGAA CTTATCCATA GGGATTTTAA AGATAACAAA 
AACAACACAG AGGGCTTTCA GGGTCTCCTG GGACATGACG TTAACTATGT CATTCAGGAG 
ACAAGAGGAG AAATGGAAGA CAGTGAACTT GATACACAGT ATTTGCAGAA TACGTTCAAG 
GTTTCAAAGC GTCAGACATT TGCTCTGTTT TCCAATCCAG GAAATCCACA AAAGGAATGT 
GCCACAGTCT TTGCCCACTC GGGGTCCTTA AAGGATCAAA GTCCAAGAGA CCCCCTCAAA 
TGCAGACAAA AAGAAGACAG TCGGGGAAAG AGTGAGTCTA AAAGCCAGCA TGTGCAGGCC 
GTTTGTACAA CAGTGCACTT TCCTGTGGCT GGTCAGAAAG ATAAGACGCC GGGTGACGAT 
GCCAAATGTA GTGCAAAAGA AGTCACTAGG GTTTGTCAGT CATTACAATT GAGAGGCCAC 
AAAACTGAAC TTGTTTTTGC TAATAAGCAA GGGGTTTCAG AAAAACCAAA TCTAACACCA 
TCACTTTTTC CCATCAAGTC ATCTGTTAAA ACCGTATGTA AGAAAAGCCC ATCAGAGAAA 
TTTGAGGAAC CTGTAATGTC ACCTGAAAGA ACATTGGGAA GTGAGAGCAT CATTCAAAGT 
GCAGTGAGCA CAATCAGCCA AAATGACATT CAAGGAAGCA CTTTTAAAGA AGTCAACTCA 
AGCAGTGTTA ATGAAGTAGG TTCCAGTACT AATGAAGTAG GCTCTAGTAT TAATGAAGTA 
GGTTCCAGTG GTGAAAACAT TCAAGCAGAA CCAGGTAGAA ACAGAGAACC TAAATTAAGA 
GCTTTACTCA GATTAGGTCT TATGCAACCT GAAGTCTATA AGCAAAGCCT TCCTGTAAGT 
AACTGTCACC ATCCTGAAAT AAAAAGGCAA GGAGAAAATG AGGACATGCT TCAGGCTGTT 
AAGGCAGATT TCTCCCCATG TCTAATTTCA GATAACCTCG AACAACCTAT GGGAAGCTGT 
CATGCTTCTC AGGTTTGTTC TGAGACACCT GACAACTTGT TAAATGACGA CGAAATAAAA 
GAAAATAGCC ACTTTGCTGA AAGTGACATT AAGGAAAGAT CTGCTGTTTT TAGTGAAAGT 
GTCCAAAAAG AATTCAGAGG GAGCCCTGGC CCTTTCACCC ATACACATTT GGCTCAGGGT 
CACCAAAGAG GGGCTGGGAA ACTAGAGTCA GAAGAGACTG TGTCTAGTGA GGATGAAGAG 
CTTCCCTGCT TCCAACAATT ACTGTTTGGT AAAGTAACCG ATACACTGTC TCCATCTACT 
GGATGGAACA CTATTGCCAC TGAGGGTCTG TCTAAGGAAA CAGAGGAGAA CCTAGAATCA 
TTGAAGAGTG GCTTAAATGA CTGCAGTGGC CAGGGAACAT CAGCCAAGGT ATCCCAGGAA 
CATCACCTTA ATGAGGAAGT GAGATGTTCT GGTAGCTTAT TTTCCTCACA GTACAGTGCA 
GTGGAAGACT TGACCACTAA TACAAACACC CAGGATCCTT TCTTGATGTT TGAGCGTCCT 
TCCAAACAAG TGTATCAGTC TGAAAGCGAG GAAGTTCTGA GTGACAAGGA ATTTGTTTCA 
GATGATGAAG AAAGGGAAAC TGGCTTGGAA GAAGATAGTT GCCAAGAAGA GCAGAGTGTG 
GATTCAGACT TAGGTGAAGC AGCATCCGAC CATGTGAGTG AAACAAGCCT CTCTGAAGAC 
TGTGTGGGGC TGTCCTCTCA GAGTGATATC TTAACCACTC AGCAGAGGGA TACCATGCAA 
GATAACCTTC TAAAGCTCCA GCAGGAAATG GCCGAGCTGG AAGCTGTGTT AGAGCAGCAC 
GGGAGCCAGC CTTCCCACAG CTCTGCTTCC CTCACAGCTG ATTCTCGTGG CCCAGAGCAC 
CTGCTAAATC TAGAGCAAGA CACATCAGAA AGAGCAATAT TAACTTCAGA GAAAAGTAGA 
GACTGTTCTA GAAGCCAGAA TCCTGAAAGC CTGTCTGCTG ACAAGTTTCC AGTATCTCTG 
GATAGTTCTA CCAATAAAAA TAAAGAACCA GGAATGGAAA GATCTTCCCC TTCTAAATCC 
CAGTTGCCGG GTAATAGGTG GTATATGCAC AGCTCAAGGA GTCTTCAGGA CAGAAACTGC 
CCATCTCAAA AGAAGCGCAT TAATGTTGCT GACATGCAGG AGCAACAACT GGCTAAGTGT 
GAGGCCCAAG ATTTAATGGG GTCTTATTTG CCGAGACAAG ATCAAGAGGG AACCCCTTAC 
CTGAAATCTG GAATCAGCCT CTTCTCTCAT GAACCCGAGT CTGATCCCTC TGAAGACAGA 
GCCCCAGAGC CAGCTCATGT TCACAGCATG CCACCCACAG CCTCTGCACT GAAATTATCC 
CAATTTCGAG TAGAAGAATC TACCAAGAAT CCAGCTGCTG CTCACATTAC TAATACTACA 
GGGTGTAACC TGAGGGAAGA AAGTGTGAGC AAAGAGAAGC CAGAAGTGTT ATCTTCAACA 
GAAAGAAGCA ACAAAAGACT TTCTATGGTG GCATCAGGCT TGACCCCAAA AGAACTCATG 
CTTGTGCAGA AATTTGCCAG AAAACATCAC GTCACTTTAA CTAATCTAAT TACTGAAGAG 
ACTACTCATG TCATTATGAA AACAGATCCT GAGTTTGTGT GTGAACGGAC ACTGAAATAT 
TTTCTGGGAA TTGCAGGAGG AAAATGGGTA GTTAGCTATT TTTGGGTCAT CCAGTCTATT 
AAAGAAAGAA AGATGCTGGA TGAGCATGAT TTTGAAGTCA GAGGAGATGT TGTCAATGGA 
AGAAACCATC AAGGCCCAAA ACGAGCAAGA GAATCCGGGG ACAAGAAGAT CTTCAAGGGC 
CTGGAAATCT GTTGCTATGG ACCTTTTACC AATATGCCCA CAGATCAACT AGAATGGATG 
GTGCAGCTGT GTGGGGCTTC TGTGGTGAAG GAGCCCTCAT CATTCACCCC AGGCCAGGGT 
ACTCACCCAG TTGTGGTTGT GCAGCCGGAT GCCTGGACAG AGGATGCTGG CTTCCATGTG 
ATCGGGCAGA TGTGTGAAGC ACCTGTGGTA ACCCGAGAGT GGGTACTGGA CAGTGTGGCC 
CTTTACCAGT GCCAGGAGCT GGACACCTAC CTGGTCCCCC AGGTCGCCCA GAGCTGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015109531.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014965017.1 Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X2, mRNA.

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CloneID	OOv27528
Clone ID Related Accession (Same CDS sequence)	XM_015109531.1 , XM_015109532.1 , XM_015109530.1
Accession Version	XM_015109531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAATATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATGCAG CTGAGACGCA TCCAGAAAAG TATCAGGGTA TTTCTGTTTC AGACTTTCAC 
GTGGAGCCAT GTGGCACAGA TACTCATGCC AGCTCATTAC AGCATGAGAA CAGCAGTTTA 
TTGCTCACTG AAAACAGACT GAATGTAGAA AAGGCTGAAT TCTGTAATAA AAGCAAACAG 
CCTGTCTTAG TAAAGAGCCA ACAGAGCAGA TGGGCTGAAA GTAAGGGCAC ATGTAAGGAT 
AGGCAGATTC CCAGCACTGG GAAAAAGATA GTTCTGAATA CTGATCCCCT GTATGGAAGA 
AAAGAACTGT GTAAGCAGAA ACCTGCATGC TCTGACAGTC CTGGAGATTC CCAAGATGTT 
CCTTGGGTAA CCCTGAATAA TAGCATACAG AAAGTTAATG ACTGGTTTTC CAGAAGTGAT 
GAAATATTAA CTTCTGACGA CTCGTGCGAT GGGGGGTCTG AATCAAATAA TGAAGTAGCT 
GGTGCAGTGG AAATTCCAAA TAACGTTGAT GGATATTCAG GTTCTTCAGA GAAAATTGAC 
TTAATGGCCA GTGATCCTCA TGGTACTTTA ATACATGAAA GAGTCCACTC CAAACCAGTA 
GAGAGTAATA TTGAAGATAA AATATTTGGG AAAACCTATC GGAGGAAGTC GAGTCTCCCT 
AACTTCAACC ACATAGCTGA AGATCTAATT ATAGGTGCAT TTACTGTAGA ACCTCAGAAA 
ACACAAGAGC AGCCCCTCAC AAATAAGCTA AAATGTAAAA GGAGAGGTAC ATCAGGCCTT 
CAGCCTGAGG ATTTTATCAA GAAAGGTGAT TTGACGATTG TTCCAAAGAC TCCTGAAAAG 
ATGATTGAGA GAACTGACCA AACAGAGCAG AAACATCACG GGATAAATAT TACTAGTGAT 
GGTCATGAGA ATAAAACAAA ACGTGATGAT GTTCAGAAAG AGCAAAACGC TAACCCAACA 
GAATCATTGG AAAACGAATC TGTTTTCAGA ACTAAGGCTG AACCTATAAA CACCAGTATA 
AGCAATATGG AACTAGAATT GAATGTCCGC AGTTCAAAAG CACCTAAGAA TAGGCTGAAG 
AGGAAATCCT CTACCAGGAA AATTCCTGAA CTTGAACTAG TAGTCAGTAG AAATCCAAGT 
CCACCTAATC ATACTGAGCT ACTAATTGAT AGCAGTTCTA GCAATGAAGA GATGAAGAAA 
AAGCATTCTA GCCAAATGCC AGTCAGGCAC AGCCAAAAGC TTCAACTCAT GGGAGATAAA 
GAACCTACAG CTGGAGCCAA GAAGAGTAAC AAGACATATG AACAAATAAA TAAAAGACTT 
GCTAGTGATG CTTTTCCAGA ACTAAATTTT GCAAACACAC CTGGTTATTT TACTAACTGT 
TCTAGTAAAC CTGAAGAGTT TGTTCATCCT AGCCTTCAAA GAGAGGAGAA TCTAGGAACA 
ATTCAAGTGT TGAATAATAC CAAAGACCCC AAAGATCTGA TATTAAGAGG AGGAAAAGCT 
TTGCAAATGG AAAGATCTGT AGAGAATACC AATATTTCCT TGGTTCCTGA TACTGATTAT 
AGCACTCAGG ATAGTATCTC ATTACTAGAA GCTAAAACCC CAGAGAAGGC AAAGACTGCA 
CCAAATCCAT GTGTGAGTCT GTGTGCAGCA ATCGAAAACC CCAAGGAACT TATCCATAGG 
GATTTTAAAG ATAACAAAAA CAACACAGAG GGCTTTCAGG GTCTCCTGGG ACATGACGTT 
AACTATGTCA TTCAGGAGAC AAGAGGAGAA ATGGAAGACA GTGAACTTGA TACACAGTAT 
TTGCAGAATA CGTTCAAGGT TTCAAAGCGT CAGACATTTG CTCTGTTTTC CAATCCAGGA 
AATCCACAAA AGGAATGTGC CACAGTCTTT GCCCACTCGG GGTCCTTAAA GGATCAAAGT 
CCAAGAGACC CCCTCAAATG CAGACAAAAA GAAGACAGTC GGGGAAAGAG TGAGTCTAAA 
AGCCAGCATG TGCAGGCCGT TTGTACAACA GTGCACTTTC CTGTGGCTGG TCAGAAAGAT 
AAGACGCCGG GTGACGATGC CAAATGTAGT GCAAAAGAAG TCACTAGGGT TTGTCAGTCA 
TTACAATTGA GAGGCCACAA AACTGAACTT GTTTTTGCTA ATAAGCAAGG GGTTTCAGAA 
AAACCAAATC TAACACCATC ACTTTTTCCC ATCAAGTCAT CTGTTAAAAC CGTATGTAAG 
AAAAGCCCAT CAGAGAAATT TGAGGAACCT GTAATGTCAC CTGAAAGAAC ATTGGGAAGT 
GAGAGCATCA TTCAAAGTGC AGTGAGCACA ATCAGCCAAA ATGACATTCA AGAAAGCACT 
TTTAAAGAAG TCAACTCAAG CAGTGTTAAT GAAGTAGGTT CCAGTACTAA TGAAGTAGGC 
TCTAGTATTA ATGAAGTAGG TTCCAGTGGT GAAAACATTC AAGCAGAACC AGGTAGAAAC 
AGAGAACCTA AATTAAGAGC TTTACTCAGA TTAGGTCTTA TGCAACCTGA AGTCTATAAG 
CAAAGCCTTC CTGTAAGTAA CTGTCACCAT CCTGAAATAA AAAGGCAAGG AGAAAATGAG 
GACATGCTTC AGGCTGTTAA GGCAGATTTC TCCCCATGTC TAATTTCAGA TAACCTCGAA 
CAACCTATGG GAAGCTGTCA TGCTTCTCAG GTTTGTTCTG AGACACCTGA CAACTTGTTA 
AATGACGACG AAATAAAAGA AAATAGCCAC TTTGCTGAAA GTGACATTAA GGAAAGATCT 
GCTGTTTTTA GTGAAAGTGT CCAAAAAGGA GAATTCAGAG GGAGCCCTGG CCCTTTCACC 
CATACACATT TGGCTCAGGG TCACCAAAGA GGGGCTGGGA AACTAGAGTC AGAAGAGACT 
GCGTCTAGTG AGGATGAAGA GCTTCCCTGC TTCCAACAAT TACTGTTTGG TAAAGTAACC 
GATACACTGT CTCCATCTAC TGGATGGAAC ACTATTGCCA CTGAGGGTCT GTCTAAGGAA 
ACAGAGGAGA ACCTAGAATC ATTGAAGAGT GGCTTAAATG ACTGCAGTGG CCAGGGAACA 
TCAGCCAAGG TATCCCAGGA ACATCACCTT AATGAGGAAG TGAGATGTTC TGGTAGCTTA 
TTTTCCTCAC AGTACAGTGC AGTGGAAGAC TTGACCACTA ATACAAACAC CCAGGATCCT 
TTCTTGATGT TTGAGCGTCC TTCCAAACAA GTGTATCAGT CTGAAAGCGA GGAAGTTCTG 
AGTGACAAGG AATTTGTTTC AGATGATGAA GAAAGGGAAA CTGGCTTGGA AGAAGATAGT 
TGCCAAGAAG AGCAGAGTGT GGATTCAGAC TTAGGTGAAG CAGCATCCGA CCATGTGAGT 
GAAACAAGCC TCTCTGAAGA CTGTGTGGGG CTGTCCTCTC AGAGTGATAT CTTAACCACT 
CAGCAGAGGG ATACCATGCA AGATAACCTT CTAAAGCTCC AGCAGGAAAT GGCCGAGCTG 
GAAGCTGTGT TAGAGCAGCA CGGGAGCCAG CCTTCCCACA GCTCTGCTTC CCTCACAGCT 
GATTCTCGTG GCCCAGAGCA CCTGCTAAAT CTAGAGCAAG ACACATCAGA AAGAGCAATA 
TTAACTTCAG AGAAAAGTAG AGACTGTTCT AGAAGCCAGA CTCCTGAAAG CCTGTCTGCT 
GACAAGTTTC CAGTATCTCT GGATAGTTCT ACCAATAAAA ATAAAGAACC AGGAATGGAA 
AGATCTTCCC CTTCTAAATC CCAGTTGCCG GGTAATAGGT GGTATATGCA CAGCTCAAGG 
AGTCTTCAGG ACAGAAACTG CCCATCTCAA AAGAAGCGCA TTAATGTTGC TGACATGCAG 
GAGCAACAAC TGGCTAAGTG TGAGGCCCAA GATTTAATGG GGTCTTATTT GCCGAGACAA 
GATCAAGAGG GAACCCCTTA CCTGAAATCT GGAATCAGCC TCTTCTCTCA TGAACCCGAG 
TCTGATCCCT CTGAAGACAG AGCCCCAGAG CCAGCTCATG TTCACAGCAT GCCACCCACA 
GCCTCTGCAC TGAAATTATC CCAATTTCGA GTAGAAGAAT CTACCAAGAA TCCAGCTGCT 
GCTCACATTA CTAATACTAC AGGGTGTAAC CTGAGGGAAG AAAGTGTGAG CAAAGAGAAG 
CCAGAAGTGT TATCTTCAAC AGAAAGAAGC AACAAAAGAC TTTCTATGGT GGCATCAGGC 
TTGACCCCAA AAGAACTCAT GCTTGTGCAG AAATTTGCCA GAAAACATCA CGTCACTTTA 
ACTAATCTAA TTACTGAAGA GACTACTCAT GTCATTATGA AAACAGATCC TGAGTTTGTG 
TGTGAACGGA CACTGAAATA TTTTCTGGGA ATTGCAGGAG GAAAATGGGT AGTTAGCTAT 
TTTTGGGTCA TCCAGTCTAT TAAAGAAAGA AAGATGCTGG ATGAGCATGA TTTTGAAGTC 
AGAGGAGATG TTGTCAATGG AAGAAACCAT CAAGGCCCAA AACGAGCAAG AGAATCCCGG 
GACAAGAAGA TCTTCAAGGG CCTGGAAATC TGTTGCTATG GACCTTTTAC CAATATGCCC 
ACAGATCAAC TAGAATGGAT GGTGCAGCTG TGTGGGGCTT CTGTGGTGAA GGAGCCCTCA 
TCATTCACCC CAGGCCAGGG TACTCACCCA GTTGTGGTTG TGCAGCCGGA TGCCTGGACA 
GAGGATGCTG GCTTCCATGT GATCGGGCAG ATGTGTGAAG CACCTGTGGT AACCCGAGAG 
TGGGTACTGG ACAGTGTGGC CCTTTACCAG TGCCAGGAGC TGGACACCTA CCTGGTCCCC 
CAGGTCGCCC AGAGCTGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014965017.1
CDS	207..5810
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLNIKDLITTQEHAAEMHAAETHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQESTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESEETASSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQTPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015109531.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015109531.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAATATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATGCAG CTGAGACGCA TCCAGAAAAG TATCAGGGTA TTTCTGTTTC AGACTTTCAC 
GTGGAGCCAT GTGGCACAGA TACTCATGCC AGCTCATTAC AGCATGAGAA CAGCAGTTTA 
TTGCTCACTG AAAACAGACT GAATGTAGAA AAGGCTGAAT TCTGTAATAA AAGCAAACAG 
CCTGTCTTAG TAAAGAGCCA ACAGAGCAGA TGGGCTGAAA GTAAGGGCAC ATGTAAGGAT 
AGGCAGATTC CCAGCACTGG GAAAAAGATA GTTCTGAATA CTGATCCCCT GTATGGAAGA 
AAAGAACTGT GTAAGCAGAA ACCTGCATGC TCTGACAGTC CTGGAGATTC CCAAGATGTT 
CCTTGGGTAA CCCTGAATAA TAGCATACAG AAAGTTAATG ACTGGTTTTC CAGAAGTGAT 
GAAATATTAA CTTCTGACGA CTCGTGCGAT GGGGGGTCTG AATCAAATAA TGAAGTAGCT 
GGTGCAGTGG AAATTCCAAA TAACGTTGAT GGATATTCAG GTTCTTCAGA GAAAATTGAC 
TTAATGGCCA GTGATCCTCA TGGTACTTTA ATACATGAAA GAGTCCACTC CAAACCAGTA 
GAGAGTAATA TTGAAGATAA AATATTTGGG AAAACCTATC GGAGGAAGTC GAGTCTCCCT 
AACTTCAACC ACATAGCTGA AGATCTAATT ATAGGTGCAT TTACTGTAGA ACCTCAGAAA 
ACACAAGAGC AGCCCCTCAC AAATAAGCTA AAATGTAAAA GGAGAGGTAC ATCAGGCCTT 
CAGCCTGAGG ATTTTATCAA GAAAGGTGAT TTGACGATTG TTCCAAAGAC TCCTGAAAAG 
ATGATTGAGA GAACTGACCA AACAGAGCAG AAACATCACG GGATAAATAT TACTAGTGAT 
GGTCATGAGA ATAAAACAAA ACGTGATGAT GTTCAGAAAG AGCAAAACGC TAACCCAACA 
GAATCATTGG AAAACGAATC TGTTTTCAGA ACTAAGGCTG AACCTATAAA CACCAGTATA 
AGCAATATGG AACTAGAATT GAATGTCCGC AGTTCAAAAG CACCTAAGAA TAGGCTGAAG 
AGGAAATCCT CTACCAGGAA AATTCCTGAA CTTGAACTAG TAGTCAGTAG AAATCCAAGT 
CCACCTAATC ATACTGAGCT ACTAATTGAT AGCAGTTCTA GCAATGAAGA GATGAAGAAA 
AAGCATTCTA GCCAAATGCC AGTCAGGCAC AGCCAAAAGC TTCAACTCAT GGGAGATAAA 
GAACCTACAG CTGGAGCCAA GAAGAGTAAC AAGACATATG AACAAATAAA TAAAAGACTT 
GCTAGTGATG CTTTTCCAGA ACTAAATTTT GCAAACACAC CTGGTTATTT TACTAACTGT 
TCTAGTAAAC CTGAAGAGTT TGTTCATCCT AGCCTTCAAA GAGAGGAGAA TCTAGGAACA 
ATTCAAGTGT TGAATAATAC CAAAGACCCC AAAGATCTGA TATTAAGAGG AGGAAAAGCT 
TTGCAAATGG AAAGATCTGT AGAGAATACC AATATTTCCT TGGTTCCTGA TACTGATTAT 
AGCACTCAGG ATAGTATCTC ATTACTAGAA GCTAAAACCC CAGAGAAGGC AAAGACTGCA 
CCAAATCCAT GTGTGAGTCT GTGTGCAGCA ATCGAAAACC CCAAGGAACT TATCCATAGG 
GATTTTAAAG ATAACAAAAA CAACACAGAG GGCTTTCAGG GTCTCCTGGG ACATGACGTT 
AACTATGTCA TTCAGGAGAC AAGAGGAGAA ATGGAAGACA GTGAACTTGA TACACAGTAT 
TTGCAGAATA CGTTCAAGGT TTCAAAGCGT CAGACATTTG CTCTGTTTTC CAATCCAGGA 
AATCCACAAA AGGAATGTGC CACAGTCTTT GCCCACTCGG GGTCCTTAAA GGATCAAAGT 
CCAAGAGACC CCCTCAAATG CAGACAAAAA GAAGACAGTC GGGGAAAGAG TGAGTCTAAA 
AGCCAGCATG TGCAGGCCGT TTGTACAACA GTGCACTTTC CTGTGGCTGG TCAGAAAGAT 
AAGACGCCGG GTGACGATGC CAAATGTAGT GCAAAAGAAG TCACTAGGGT TTGTCAGTCA 
TTACAATTGA GAGGCCACAA AACTGAACTT GTTTTTGCTA ATAAGCAAGG GGTTTCAGAA 
AAACCAAATC TAACACCATC ACTTTTTCCC ATCAAGTCAT CTGTTAAAAC CGTATGTAAG 
AAAAGCCCAT CAGAGAAATT TGAGGAACCT GTAATGTCAC CTGAAAGAAC ATTGGGAAGT 
GAGAGCATCA TTCAAAGTGC AGTGAGCACA ATCAGCCAAA ATGACATTCA AGAAAGCACT 
TTTAAAGAAG TCAACTCAAG CAGTGTTAAT GAAGTAGGTT CCAGTACTAA TGAAGTAGGC 
TCTAGTATTA ATGAAGTAGG TTCCAGTGGT GAAAACATTC AAGCAGAACC AGGTAGAAAC 
AGAGAACCTA AATTAAGAGC TTTACTCAGA TTAGGTCTTA TGCAACCTGA AGTCTATAAG 
CAAAGCCTTC CTGTAAGTAA CTGTCACCAT CCTGAAATAA AAAGGCAAGG AGAAAATGAG 
GACATGCTTC AGGCTGTTAA GGCAGATTTC TCCCCATGTC TAATTTCAGA TAACCTCGAA 
CAACCTATGG GAAGCTGTCA TGCTTCTCAG GTTTGTTCTG AGACACCTGA CAACTTGTTA 
AATGACGACG AAATAAAAGA AAATAGCCAC TTTGCTGAAA GTGACATTAA GGAAAGATCT 
GCTGTTTTTA GTGAAAGTGT CCAAAAAGGA GAATTCAGAG GGAGCCCTGG CCCTTTCACC 
CATACACATT TGGCTCAGGG TCACCAAAGA GGGGCTGGGA AACTAGAGTC AGAAGAGACT 
GCGTCTAGTG AGGATGAAGA GCTTCCCTGC TTCCAACAAT TACTGTTTGG TAAAGTAACC 
GATACACTGT CTCCATCTAC TGGATGGAAC ACTATTGCCA CTGAGGGTCT GTCTAAGGAA 
ACAGAGGAGA ACCTAGAATC ATTGAAGAGT GGCTTAAATG ACTGCAGTGG CCAGGGAACA 
TCAGCCAAGG TATCCCAGGA ACATCACCTT AATGAGGAAG TGAGATGTTC TGGTAGCTTA 
TTTTCCTCAC AGTACAGTGC AGTGGAAGAC TTGACCACTA ATACAAACAC CCAGGATCCT 
TTCTTGATGT TTGAGCGTCC TTCCAAACAA GTGTATCAGT CTGAAAGCGA GGAAGTTCTG 
AGTGACAAGG AATTTGTTTC AGATGATGAA GAAAGGGAAA CTGGCTTGGA AGAAGATAGT 
TGCCAAGAAG AGCAGAGTGT GGATTCAGAC TTAGGTGAAG CAGCATCCGA CCATGTGAGT 
GAAACAAGCC TCTCTGAAGA CTGTGTGGGG CTGTCCTCTC AGAGTGATAT CTTAACCACT 
CAGCAGAGGG ATACCATGCA AGATAACCTT CTAAAGCTCC AGCAGGAAAT GGCCGAGCTG 
GAAGCTGTGT TAGAGCAGCA CGGGAGCCAG CCTTCCCACA GCTCTGCTTC CCTCACAGCT 
GATTCTCGTG GCCCAGAGCA CCTGCTAAAT CTAGAGCAAG ACACATCAGA AAGAGCAATA 
TTAACTTCAG AGAAAAGTAG AGACTGTTCT AGAAGCCAGA CTCCTGAAAG CCTGTCTGCT 
GACAAGTTTC CAGTATCTCT GGATAGTTCT ACCAATAAAA ATAAAGAACC AGGAATGGAA 
AGATCTTCCC CTTCTAAATC CCAGTTGCCG GGTAATAGGT GGTATATGCA CAGCTCAAGG 
AGTCTTCAGG ACAGAAACTG CCCATCTCAA AAGAAGCGCA TTAATGTTGC TGACATGCAG 
GAGCAACAAC TGGCTAAGTG TGAGGCCCAA GATTTAATGG GGTCTTATTT GCCGAGACAA 
GATCAAGAGG GAACCCCTTA CCTGAAATCT GGAATCAGCC TCTTCTCTCA TGAACCCGAG 
TCTGATCCCT CTGAAGACAG AGCCCCAGAG CCAGCTCATG TTCACAGCAT GCCACCCACA 
GCCTCTGCAC TGAAATTATC CCAATTTCGA GTAGAAGAAT CTACCAAGAA TCCAGCTGCT 
GCTCACATTA CTAATACTAC AGGGTGTAAC CTGAGGGAAG AAAGTGTGAG CAAAGAGAAG 
CCAGAAGTGT TATCTTCAAC AGAAAGAAGC AACAAAAGAC TTTCTATGGT GGCATCAGGC 
TTGACCCCAA AAGAACTCAT GCTTGTGCAG AAATTTGCCA GAAAACATCA CGTCACTTTA 
ACTAATCTAA TTACTGAAGA GACTACTCAT GTCATTATGA AAACAGATCC TGAGTTTGTG 
TGTGAACGGA CACTGAAATA TTTTCTGGGA ATTGCAGGAG GAAAATGGGT AGTTAGCTAT 
TTTTGGGTCA TCCAGTCTAT TAAAGAAAGA AAGATGCTGG ATGAGCATGA TTTTGAAGTC 
AGAGGAGATG TTGTCAATGG AAGAAACCAT CAAGGCCCAA AACGAGCAAG AGAATCCCGG 
GACAAGAAGA TCTTCAAGGG CCTGGAAATC TGTTGCTATG GACCTTTTAC CAATATGCCC 
ACAGATCAAC TAGAATGGAT GGTGCAGCTG TGTGGGGCTT CTGTGGTGAA GGAGCCCTCA 
TCATTCACCC CAGGCCAGGG TACTCACCCA GTTGTGGTTG TGCAGCCGGA TGCCTGGACA 
GAGGATGCTG GCTTCCATGT GATCGGGCAG ATGTGTGAAG CACCTGTGGT AACCCGAGAG 
TGGGTACTGG ACAGTGTGGC CCTTTACCAG TGCCAGGAGC TGGACACCTA CCTGGTCCCC 
CAGGTCGCCC AGAGCTGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015109532.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014965018.1 Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X3, mRNA.

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CloneID	OOv27528
Clone ID Related Accession (Same CDS sequence)	XM_015109531.1 , XM_015109532.1 , XM_015109530.1
Accession Version	XM_015109532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAATATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATGCAG CTGAGACGCA TCCAGAAAAG TATCAGGGTA TTTCTGTTTC AGACTTTCAC 
GTGGAGCCAT GTGGCACAGA TACTCATGCC AGCTCATTAC AGCATGAGAA CAGCAGTTTA 
TTGCTCACTG AAAACAGACT GAATGTAGAA AAGGCTGAAT TCTGTAATAA AAGCAAACAG 
CCTGTCTTAG TAAAGAGCCA ACAGAGCAGA TGGGCTGAAA GTAAGGGCAC ATGTAAGGAT 
AGGCAGATTC CCAGCACTGG GAAAAAGATA GTTCTGAATA CTGATCCCCT GTATGGAAGA 
AAAGAACTGT GTAAGCAGAA ACCTGCATGC TCTGACAGTC CTGGAGATTC CCAAGATGTT 
CCTTGGGTAA CCCTGAATAA TAGCATACAG AAAGTTAATG ACTGGTTTTC CAGAAGTGAT 
GAAATATTAA CTTCTGACGA CTCGTGCGAT GGGGGGTCTG AATCAAATAA TGAAGTAGCT 
GGTGCAGTGG AAATTCCAAA TAACGTTGAT GGATATTCAG GTTCTTCAGA GAAAATTGAC 
TTAATGGCCA GTGATCCTCA TGGTACTTTA ATACATGAAA GAGTCCACTC CAAACCAGTA 
GAGAGTAATA TTGAAGATAA AATATTTGGG AAAACCTATC GGAGGAAGTC GAGTCTCCCT 
AACTTCAACC ACATAGCTGA AGATCTAATT ATAGGTGCAT TTACTGTAGA ACCTCAGAAA 
ACACAAGAGC AGCCCCTCAC AAATAAGCTA AAATGTAAAA GGAGAGGTAC ATCAGGCCTT 
CAGCCTGAGG ATTTTATCAA GAAAGGTGAT TTGACGATTG TTCCAAAGAC TCCTGAAAAG 
ATGATTGAGA GAACTGACCA AACAGAGCAG AAACATCACG GGATAAATAT TACTAGTGAT 
GGTCATGAGA ATAAAACAAA ACGTGATGAT GTTCAGAAAG AGCAAAACGC TAACCCAACA 
GAATCATTGG AAAACGAATC TGTTTTCAGA ACTAAGGCTG AACCTATAAA CACCAGTATA 
AGCAATATGG AACTAGAATT GAATGTCCGC AGTTCAAAAG CACCTAAGAA TAGGCTGAAG 
AGGAAATCCT CTACCAGGAA AATTCCTGAA CTTGAACTAG TAGTCAGTAG AAATCCAAGT 
CCACCTAATC ATACTGAGCT ACTAATTGAT AGCAGTTCTA GCAATGAAGA GATGAAGAAA 
AAGCATTCTA GCCAAATGCC AGTCAGGCAC AGCCAAAAGC TTCAACTCAT GGGAGATAAA 
GAACCTACAG CTGGAGCCAA GAAGAGTAAC AAGACATATG AACAAATAAA TAAAAGACTT 
GCTAGTGATG CTTTTCCAGA ACTAAATTTT GCAAACACAC CTGGTTATTT TACTAACTGT 
TCTAGTAAAC CTGAAGAGTT TGTTCATCCT AGCCTTCAAA GAGAGGAGAA TCTAGGAACA 
ATTCAAGTGT TGAATAATAC CAAAGACCCC AAAGATCTGA TATTAAGAGG AGGAAAAGCT 
TTGCAAATGG AAAGATCTGT AGAGAATACC AATATTTCCT TGGTTCCTGA TACTGATTAT 
AGCACTCAGG ATAGTATCTC ATTACTAGAA GCTAAAACCC CAGAGAAGGC AAAGACTGCA 
CCAAATCCAT GTGTGAGTCT GTGTGCAGCA ATCGAAAACC CCAAGGAACT TATCCATAGG 
GATTTTAAAG ATAACAAAAA CAACACAGAG GGCTTTCAGG GTCTCCTGGG ACATGACGTT 
AACTATGTCA TTCAGGAGAC AAGAGGAGAA ATGGAAGACA GTGAACTTGA TACACAGTAT 
TTGCAGAATA CGTTCAAGGT TTCAAAGCGT CAGACATTTG CTCTGTTTTC CAATCCAGGA 
AATCCACAAA AGGAATGTGC CACAGTCTTT GCCCACTCGG GGTCCTTAAA GGATCAAAGT 
CCAAGAGACC CCCTCAAATG CAGACAAAAA GAAGACAGTC GGGGAAAGAG TGAGTCTAAA 
AGCCAGCATG TGCAGGCCGT TTGTACAACA GTGCACTTTC CTGTGGCTGG TCAGAAAGAT 
AAGACGCCGG GTGACGATGC CAAATGTAGT GCAAAAGAAG TCACTAGGGT TTGTCAGTCA 
TTACAATTGA GAGGCCACAA AACTGAACTT GTTTTTGCTA ATAAGCAAGG GGTTTCAGAA 
AAACCAAATC TAACACCATC ACTTTTTCCC ATCAAGTCAT CTGTTAAAAC CGTATGTAAG 
AAAAGCCCAT CAGAGAAATT TGAGGAACCT GTAATGTCAC CTGAAAGAAC ATTGGGAAGT 
GAGAGCATCA TTCAAAGTGC AGTGAGCACA ATCAGCCAAA ATGACATTCA AGAAAGCACT 
TTTAAAGAAG TCAACTCAAG CAGTGTTAAT GAAGTAGGTT CCAGTACTAA TGAAGTAGGC 
TCTAGTATTA ATGAAGTAGG TTCCAGTGGT GAAAACATTC AAGCAGAACC AGGTAGAAAC 
AGAGAACCTA AATTAAGAGC TTTACTCAGA TTAGGTCTTA TGCAACCTGA AGTCTATAAG 
CAAAGCCTTC CTGTAAGTAA CTGTCACCAT CCTGAAATAA AAAGGCAAGG AGAAAATGAG 
GACATGCTTC AGGCTGTTAA GGCAGATTTC TCCCCATGTC TAATTTCAGA TAACCTCGAA 
CAACCTATGG GAAGCTGTCA TGCTTCTCAG GTTTGTTCTG AGACACCTGA CAACTTGTTA 
AATGACGACG AAATAAAAGA AAATAGCCAC TTTGCTGAAA GTGACATTAA GGAAAGATCT 
GCTGTTTTTA GTGAAAGTGT CCAAAAAGGA GAATTCAGAG GGAGCCCTGG CCCTTTCACC 
CATACACATT TGGCTCAGGG TCACCAAAGA GGGGCTGGGA AACTAGAGTC AGAAGAGACT 
GCGTCTAGTG AGGATGAAGA GCTTCCCTGC TTCCAACAAT TACTGTTTGG TAAAGTAACC 
GATACACTGT CTCCATCTAC TGGATGGAAC ACTATTGCCA CTGAGGGTCT GTCTAAGGAA 
ACAGAGGAGA ACCTAGAATC ATTGAAGAGT GGCTTAAATG ACTGCAGTGG CCAGGGAACA 
TCAGCCAAGG TATCCCAGGA ACATCACCTT AATGAGGAAG TGAGATGTTC TGGTAGCTTA 
TTTTCCTCAC AGTACAGTGC AGTGGAAGAC TTGACCACTA ATACAAACAC CCAGGATCCT 
TTCTTGATGT TTGAGCGTCC TTCCAAACAA GTGTATCAGT CTGAAAGCGA GGAAGTTCTG 
AGTGACAAGG AATTTGTTTC AGATGATGAA GAAAGGGAAA CTGGCTTGGA AGAAGATAGT 
TGCCAAGAAG AGCAGAGTGT GGATTCAGAC TTAGGTGAAG CAGCATCCGA CCATGTGAGT 
GAAACAAGCC TCTCTGAAGA CTGTGTGGGG CTGTCCTCTC AGAGTGATAT CTTAACCACT 
CAGCAGAGGG ATACCATGCA AGATAACCTT CTAAAGCTCC AGCAGGAAAT GGCCGAGCTG 
GAAGCTGTGT TAGAGCAGCA CGGGAGCCAG CCTTCCCACA GCTCTGCTTC CCTCACAGCT 
GATTCTCGTG GCCCAGAGCA CCTGCTAAAT CTAGAGCAAG ACACATCAGA AAGAGCAATA 
TTAACTTCAG AGAAAAGTAG AGACTGTTCT AGAAGCCAGA CTCCTGAAAG CCTGTCTGCT 
GACAAGTTTC CAGTATCTCT GGATAGTTCT ACCAATAAAA ATAAAGAACC AGGAATGGAA 
AGATCTTCCC CTTCTAAATC CCAGTTGCCG GGTAATAGGT GGTATATGCA CAGCTCAAGG 
AGTCTTCAGG ACAGAAACTG CCCATCTCAA AAGAAGCGCA TTAATGTTGC TGACATGCAG 
GAGCAACAAC TGGCTAAGTG TGAGGCCCAA GATTTAATGG GGTCTTATTT GCCGAGACAA 
GATCAAGAGG GAACCCCTTA CCTGAAATCT GGAATCAGCC TCTTCTCTCA TGAACCCGAG 
TCTGATCCCT CTGAAGACAG AGCCCCAGAG CCAGCTCATG TTCACAGCAT GCCACCCACA 
GCCTCTGCAC TGAAATTATC CCAATTTCGA GTAGAAGAAT CTACCAAGAA TCCAGCTGCT 
GCTCACATTA CTAATACTAC AGGGTGTAAC CTGAGGGAAG AAAGTGTGAG CAAAGAGAAG 
CCAGAAGTGT TATCTTCAAC AGAAAGAAGC AACAAAAGAC TTTCTATGGT GGCATCAGGC 
TTGACCCCAA AAGAACTCAT GCTTGTGCAG AAATTTGCCA GAAAACATCA CGTCACTTTA 
ACTAATCTAA TTACTGAAGA GACTACTCAT GTCATTATGA AAACAGATCC TGAGTTTGTG 
TGTGAACGGA CACTGAAATA TTTTCTGGGA ATTGCAGGAG GAAAATGGGT AGTTAGCTAT 
TTTTGGGTCA TCCAGTCTAT TAAAGAAAGA AAGATGCTGG ATGAGCATGA TTTTGAAGTC 
AGAGGAGATG TTGTCAATGG AAGAAACCAT CAAGGCCCAA AACGAGCAAG AGAATCCCGG 
GACAAGAAGA TCTTCAAGGG CCTGGAAATC TGTTGCTATG GACCTTTTAC CAATATGCCC 
ACAGATCAAC TAGAATGGAT GGTGCAGCTG TGTGGGGCTT CTGTGGTGAA GGAGCCCTCA 
TCATTCACCC CAGGCCAGGG TACTCACCCA GTTGTGGTTG TGCAGCCGGA TGCCTGGACA 
GAGGATGCTG GCTTCCATGT GATCGGGCAG ATGTGTGAAG CACCTGTGGT AACCCGAGAG 
TGGGTACTGG ACAGTGTGGC CCTTTACCAG TGCCAGGAGC TGGACACCTA CCTGGTCCCC 
CAGGTCGCCC AGAGCTGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014965018.1
CDS	196..5799
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLNIKDLITTQEHAAEMHAAETHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQESTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESEETASSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQTPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015109532.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015109532.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAATATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATGCAG CTGAGACGCA TCCAGAAAAG TATCAGGGTA TTTCTGTTTC AGACTTTCAC 
GTGGAGCCAT GTGGCACAGA TACTCATGCC AGCTCATTAC AGCATGAGAA CAGCAGTTTA 
TTGCTCACTG AAAACAGACT GAATGTAGAA AAGGCTGAAT TCTGTAATAA AAGCAAACAG 
CCTGTCTTAG TAAAGAGCCA ACAGAGCAGA TGGGCTGAAA GTAAGGGCAC ATGTAAGGAT 
AGGCAGATTC CCAGCACTGG GAAAAAGATA GTTCTGAATA CTGATCCCCT GTATGGAAGA 
AAAGAACTGT GTAAGCAGAA ACCTGCATGC TCTGACAGTC CTGGAGATTC CCAAGATGTT 
CCTTGGGTAA CCCTGAATAA TAGCATACAG AAAGTTAATG ACTGGTTTTC CAGAAGTGAT 
GAAATATTAA CTTCTGACGA CTCGTGCGAT GGGGGGTCTG AATCAAATAA TGAAGTAGCT 
GGTGCAGTGG AAATTCCAAA TAACGTTGAT GGATATTCAG GTTCTTCAGA GAAAATTGAC 
TTAATGGCCA GTGATCCTCA TGGTACTTTA ATACATGAAA GAGTCCACTC CAAACCAGTA 
GAGAGTAATA TTGAAGATAA AATATTTGGG AAAACCTATC GGAGGAAGTC GAGTCTCCCT 
AACTTCAACC ACATAGCTGA AGATCTAATT ATAGGTGCAT TTACTGTAGA ACCTCAGAAA 
ACACAAGAGC AGCCCCTCAC AAATAAGCTA AAATGTAAAA GGAGAGGTAC ATCAGGCCTT 
CAGCCTGAGG ATTTTATCAA GAAAGGTGAT TTGACGATTG TTCCAAAGAC TCCTGAAAAG 
ATGATTGAGA GAACTGACCA AACAGAGCAG AAACATCACG GGATAAATAT TACTAGTGAT 
GGTCATGAGA ATAAAACAAA ACGTGATGAT GTTCAGAAAG AGCAAAACGC TAACCCAACA 
GAATCATTGG AAAACGAATC TGTTTTCAGA ACTAAGGCTG AACCTATAAA CACCAGTATA 
AGCAATATGG AACTAGAATT GAATGTCCGC AGTTCAAAAG CACCTAAGAA TAGGCTGAAG 
AGGAAATCCT CTACCAGGAA AATTCCTGAA CTTGAACTAG TAGTCAGTAG AAATCCAAGT 
CCACCTAATC ATACTGAGCT ACTAATTGAT AGCAGTTCTA GCAATGAAGA GATGAAGAAA 
AAGCATTCTA GCCAAATGCC AGTCAGGCAC AGCCAAAAGC TTCAACTCAT GGGAGATAAA 
GAACCTACAG CTGGAGCCAA GAAGAGTAAC AAGACATATG AACAAATAAA TAAAAGACTT 
GCTAGTGATG CTTTTCCAGA ACTAAATTTT GCAAACACAC CTGGTTATTT TACTAACTGT 
TCTAGTAAAC CTGAAGAGTT TGTTCATCCT AGCCTTCAAA GAGAGGAGAA TCTAGGAACA 
ATTCAAGTGT TGAATAATAC CAAAGACCCC AAAGATCTGA TATTAAGAGG AGGAAAAGCT 
TTGCAAATGG AAAGATCTGT AGAGAATACC AATATTTCCT TGGTTCCTGA TACTGATTAT 
AGCACTCAGG ATAGTATCTC ATTACTAGAA GCTAAAACCC CAGAGAAGGC AAAGACTGCA 
CCAAATCCAT GTGTGAGTCT GTGTGCAGCA ATCGAAAACC CCAAGGAACT TATCCATAGG 
GATTTTAAAG ATAACAAAAA CAACACAGAG GGCTTTCAGG GTCTCCTGGG ACATGACGTT 
AACTATGTCA TTCAGGAGAC AAGAGGAGAA ATGGAAGACA GTGAACTTGA TACACAGTAT 
TTGCAGAATA CGTTCAAGGT TTCAAAGCGT CAGACATTTG CTCTGTTTTC CAATCCAGGA 
AATCCACAAA AGGAATGTGC CACAGTCTTT GCCCACTCGG GGTCCTTAAA GGATCAAAGT 
CCAAGAGACC CCCTCAAATG CAGACAAAAA GAAGACAGTC GGGGAAAGAG TGAGTCTAAA 
AGCCAGCATG TGCAGGCCGT TTGTACAACA GTGCACTTTC CTGTGGCTGG TCAGAAAGAT 
AAGACGCCGG GTGACGATGC CAAATGTAGT GCAAAAGAAG TCACTAGGGT TTGTCAGTCA 
TTACAATTGA GAGGCCACAA AACTGAACTT GTTTTTGCTA ATAAGCAAGG GGTTTCAGAA 
AAACCAAATC TAACACCATC ACTTTTTCCC ATCAAGTCAT CTGTTAAAAC CGTATGTAAG 
AAAAGCCCAT CAGAGAAATT TGAGGAACCT GTAATGTCAC CTGAAAGAAC ATTGGGAAGT 
GAGAGCATCA TTCAAAGTGC AGTGAGCACA ATCAGCCAAA ATGACATTCA AGAAAGCACT 
TTTAAAGAAG TCAACTCAAG CAGTGTTAAT GAAGTAGGTT CCAGTACTAA TGAAGTAGGC 
TCTAGTATTA ATGAAGTAGG TTCCAGTGGT GAAAACATTC AAGCAGAACC AGGTAGAAAC 
AGAGAACCTA AATTAAGAGC TTTACTCAGA TTAGGTCTTA TGCAACCTGA AGTCTATAAG 
CAAAGCCTTC CTGTAAGTAA CTGTCACCAT CCTGAAATAA AAAGGCAAGG AGAAAATGAG 
GACATGCTTC AGGCTGTTAA GGCAGATTTC TCCCCATGTC TAATTTCAGA TAACCTCGAA 
CAACCTATGG GAAGCTGTCA TGCTTCTCAG GTTTGTTCTG AGACACCTGA CAACTTGTTA 
AATGACGACG AAATAAAAGA AAATAGCCAC TTTGCTGAAA GTGACATTAA GGAAAGATCT 
GCTGTTTTTA GTGAAAGTGT CCAAAAAGGA GAATTCAGAG GGAGCCCTGG CCCTTTCACC 
CATACACATT TGGCTCAGGG TCACCAAAGA GGGGCTGGGA AACTAGAGTC AGAAGAGACT 
GCGTCTAGTG AGGATGAAGA GCTTCCCTGC TTCCAACAAT TACTGTTTGG TAAAGTAACC 
GATACACTGT CTCCATCTAC TGGATGGAAC ACTATTGCCA CTGAGGGTCT GTCTAAGGAA 
ACAGAGGAGA ACCTAGAATC ATTGAAGAGT GGCTTAAATG ACTGCAGTGG CCAGGGAACA 
TCAGCCAAGG TATCCCAGGA ACATCACCTT AATGAGGAAG TGAGATGTTC TGGTAGCTTA 
TTTTCCTCAC AGTACAGTGC AGTGGAAGAC TTGACCACTA ATACAAACAC CCAGGATCCT 
TTCTTGATGT TTGAGCGTCC TTCCAAACAA GTGTATCAGT CTGAAAGCGA GGAAGTTCTG 
AGTGACAAGG AATTTGTTTC AGATGATGAA GAAAGGGAAA CTGGCTTGGA AGAAGATAGT 
TGCCAAGAAG AGCAGAGTGT GGATTCAGAC TTAGGTGAAG CAGCATCCGA CCATGTGAGT 
GAAACAAGCC TCTCTGAAGA CTGTGTGGGG CTGTCCTCTC AGAGTGATAT CTTAACCACT 
CAGCAGAGGG ATACCATGCA AGATAACCTT CTAAAGCTCC AGCAGGAAAT GGCCGAGCTG 
GAAGCTGTGT TAGAGCAGCA CGGGAGCCAG CCTTCCCACA GCTCTGCTTC CCTCACAGCT 
GATTCTCGTG GCCCAGAGCA CCTGCTAAAT CTAGAGCAAG ACACATCAGA AAGAGCAATA 
TTAACTTCAG AGAAAAGTAG AGACTGTTCT AGAAGCCAGA CTCCTGAAAG CCTGTCTGCT 
GACAAGTTTC CAGTATCTCT GGATAGTTCT ACCAATAAAA ATAAAGAACC AGGAATGGAA 
AGATCTTCCC CTTCTAAATC CCAGTTGCCG GGTAATAGGT GGTATATGCA CAGCTCAAGG 
AGTCTTCAGG ACAGAAACTG CCCATCTCAA AAGAAGCGCA TTAATGTTGC TGACATGCAG 
GAGCAACAAC TGGCTAAGTG TGAGGCCCAA GATTTAATGG GGTCTTATTT GCCGAGACAA 
GATCAAGAGG GAACCCCTTA CCTGAAATCT GGAATCAGCC TCTTCTCTCA TGAACCCGAG 
TCTGATCCCT CTGAAGACAG AGCCCCAGAG CCAGCTCATG TTCACAGCAT GCCACCCACA 
GCCTCTGCAC TGAAATTATC CCAATTTCGA GTAGAAGAAT CTACCAAGAA TCCAGCTGCT 
GCTCACATTA CTAATACTAC AGGGTGTAAC CTGAGGGAAG AAAGTGTGAG CAAAGAGAAG 
CCAGAAGTGT TATCTTCAAC AGAAAGAAGC AACAAAAGAC TTTCTATGGT GGCATCAGGC 
TTGACCCCAA AAGAACTCAT GCTTGTGCAG AAATTTGCCA GAAAACATCA CGTCACTTTA 
ACTAATCTAA TTACTGAAGA GACTACTCAT GTCATTATGA AAACAGATCC TGAGTTTGTG 
TGTGAACGGA CACTGAAATA TTTTCTGGGA ATTGCAGGAG GAAAATGGGT AGTTAGCTAT 
TTTTGGGTCA TCCAGTCTAT TAAAGAAAGA AAGATGCTGG ATGAGCATGA TTTTGAAGTC 
AGAGGAGATG TTGTCAATGG AAGAAACCAT CAAGGCCCAA AACGAGCAAG AGAATCCCGG 
GACAAGAAGA TCTTCAAGGG CCTGGAAATC TGTTGCTATG GACCTTTTAC CAATATGCCC 
ACAGATCAAC TAGAATGGAT GGTGCAGCTG TGTGGGGCTT CTGTGGTGAA GGAGCCCTCA 
TCATTCACCC CAGGCCAGGG TACTCACCCA GTTGTGGTTG TGCAGCCGGA TGCCTGGACA 
GAGGATGCTG GCTTCCATGT GATCGGGCAG ATGTGTGAAG CACCTGTGGT AACCCGAGAG 
TGGGTACTGG ACAGTGTGGC CCTTTACCAG TGCCAGGAGC TGGACACCTA CCTGGTCCCC 
CAGGTCGCCC AGAGCTGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015109530.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014965016.1 Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X1, mRNA.

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CloneID	OOv27528
Clone ID Related Accession (Same CDS sequence)	XM_015109531.1 , XM_015109532.1 , XM_015109530.1
Accession Version	XM_015109530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAATATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATGCAG CTGAGACGCA TCCAGAAAAG TATCAGGGTA TTTCTGTTTC AGACTTTCAC 
GTGGAGCCAT GTGGCACAGA TACTCATGCC AGCTCATTAC AGCATGAGAA CAGCAGTTTA 
TTGCTCACTG AAAACAGACT GAATGTAGAA AAGGCTGAAT TCTGTAATAA AAGCAAACAG 
CCTGTCTTAG TAAAGAGCCA ACAGAGCAGA TGGGCTGAAA GTAAGGGCAC ATGTAAGGAT 
AGGCAGATTC CCAGCACTGG GAAAAAGATA GTTCTGAATA CTGATCCCCT GTATGGAAGA 
AAAGAACTGT GTAAGCAGAA ACCTGCATGC TCTGACAGTC CTGGAGATTC CCAAGATGTT 
CCTTGGGTAA CCCTGAATAA TAGCATACAG AAAGTTAATG ACTGGTTTTC CAGAAGTGAT 
GAAATATTAA CTTCTGACGA CTCGTGCGAT GGGGGGTCTG AATCAAATAA TGAAGTAGCT 
GGTGCAGTGG AAATTCCAAA TAACGTTGAT GGATATTCAG GTTCTTCAGA GAAAATTGAC 
TTAATGGCCA GTGATCCTCA TGGTACTTTA ATACATGAAA GAGTCCACTC CAAACCAGTA 
GAGAGTAATA TTGAAGATAA AATATTTGGG AAAACCTATC GGAGGAAGTC GAGTCTCCCT 
AACTTCAACC ACATAGCTGA AGATCTAATT ATAGGTGCAT TTACTGTAGA ACCTCAGAAA 
ACACAAGAGC AGCCCCTCAC AAATAAGCTA AAATGTAAAA GGAGAGGTAC ATCAGGCCTT 
CAGCCTGAGG ATTTTATCAA GAAAGGTGAT TTGACGATTG TTCCAAAGAC TCCTGAAAAG 
ATGATTGAGA GAACTGACCA AACAGAGCAG AAACATCACG GGATAAATAT TACTAGTGAT 
GGTCATGAGA ATAAAACAAA ACGTGATGAT GTTCAGAAAG AGCAAAACGC TAACCCAACA 
GAATCATTGG AAAACGAATC TGTTTTCAGA ACTAAGGCTG AACCTATAAA CACCAGTATA 
AGCAATATGG AACTAGAATT GAATGTCCGC AGTTCAAAAG CACCTAAGAA TAGGCTGAAG 
AGGAAATCCT CTACCAGGAA AATTCCTGAA CTTGAACTAG TAGTCAGTAG AAATCCAAGT 
CCACCTAATC ATACTGAGCT ACTAATTGAT AGCAGTTCTA GCAATGAAGA GATGAAGAAA 
AAGCATTCTA GCCAAATGCC AGTCAGGCAC AGCCAAAAGC TTCAACTCAT GGGAGATAAA 
GAACCTACAG CTGGAGCCAA GAAGAGTAAC AAGACATATG AACAAATAAA TAAAAGACTT 
GCTAGTGATG CTTTTCCAGA ACTAAATTTT GCAAACACAC CTGGTTATTT TACTAACTGT 
TCTAGTAAAC CTGAAGAGTT TGTTCATCCT AGCCTTCAAA GAGAGGAGAA TCTAGGAACA 
ATTCAAGTGT TGAATAATAC CAAAGACCCC AAAGATCTGA TATTAAGAGG AGGAAAAGCT 
TTGCAAATGG AAAGATCTGT AGAGAATACC AATATTTCCT TGGTTCCTGA TACTGATTAT 
AGCACTCAGG ATAGTATCTC ATTACTAGAA GCTAAAACCC CAGAGAAGGC AAAGACTGCA 
CCAAATCCAT GTGTGAGTCT GTGTGCAGCA ATCGAAAACC CCAAGGAACT TATCCATAGG 
GATTTTAAAG ATAACAAAAA CAACACAGAG GGCTTTCAGG GTCTCCTGGG ACATGACGTT 
AACTATGTCA TTCAGGAGAC AAGAGGAGAA ATGGAAGACA GTGAACTTGA TACACAGTAT 
TTGCAGAATA CGTTCAAGGT TTCAAAGCGT CAGACATTTG CTCTGTTTTC CAATCCAGGA 
AATCCACAAA AGGAATGTGC CACAGTCTTT GCCCACTCGG GGTCCTTAAA GGATCAAAGT 
CCAAGAGACC CCCTCAAATG CAGACAAAAA GAAGACAGTC GGGGAAAGAG TGAGTCTAAA 
AGCCAGCATG TGCAGGCCGT TTGTACAACA GTGCACTTTC CTGTGGCTGG TCAGAAAGAT 
AAGACGCCGG GTGACGATGC CAAATGTAGT GCAAAAGAAG TCACTAGGGT TTGTCAGTCA 
TTACAATTGA GAGGCCACAA AACTGAACTT GTTTTTGCTA ATAAGCAAGG GGTTTCAGAA 
AAACCAAATC TAACACCATC ACTTTTTCCC ATCAAGTCAT CTGTTAAAAC CGTATGTAAG 
AAAAGCCCAT CAGAGAAATT TGAGGAACCT GTAATGTCAC CTGAAAGAAC ATTGGGAAGT 
GAGAGCATCA TTCAAAGTGC AGTGAGCACA ATCAGCCAAA ATGACATTCA AGAAAGCACT 
TTTAAAGAAG TCAACTCAAG CAGTGTTAAT GAAGTAGGTT CCAGTACTAA TGAAGTAGGC 
TCTAGTATTA ATGAAGTAGG TTCCAGTGGT GAAAACATTC AAGCAGAACC AGGTAGAAAC 
AGAGAACCTA AATTAAGAGC TTTACTCAGA TTAGGTCTTA TGCAACCTGA AGTCTATAAG 
CAAAGCCTTC CTGTAAGTAA CTGTCACCAT CCTGAAATAA AAAGGCAAGG AGAAAATGAG 
GACATGCTTC AGGCTGTTAA GGCAGATTTC TCCCCATGTC TAATTTCAGA TAACCTCGAA 
CAACCTATGG GAAGCTGTCA TGCTTCTCAG GTTTGTTCTG AGACACCTGA CAACTTGTTA 
AATGACGACG AAATAAAAGA AAATAGCCAC TTTGCTGAAA GTGACATTAA GGAAAGATCT 
GCTGTTTTTA GTGAAAGTGT CCAAAAAGGA GAATTCAGAG GGAGCCCTGG CCCTTTCACC 
CATACACATT TGGCTCAGGG TCACCAAAGA GGGGCTGGGA AACTAGAGTC AGAAGAGACT 
GCGTCTAGTG AGGATGAAGA GCTTCCCTGC TTCCAACAAT TACTGTTTGG TAAAGTAACC 
GATACACTGT CTCCATCTAC TGGATGGAAC ACTATTGCCA CTGAGGGTCT GTCTAAGGAA 
ACAGAGGAGA ACCTAGAATC ATTGAAGAGT GGCTTAAATG ACTGCAGTGG CCAGGGAACA 
TCAGCCAAGG TATCCCAGGA ACATCACCTT AATGAGGAAG TGAGATGTTC TGGTAGCTTA 
TTTTCCTCAC AGTACAGTGC AGTGGAAGAC TTGACCACTA ATACAAACAC CCAGGATCCT 
TTCTTGATGT TTGAGCGTCC TTCCAAACAA GTGTATCAGT CTGAAAGCGA GGAAGTTCTG 
AGTGACAAGG AATTTGTTTC AGATGATGAA GAAAGGGAAA CTGGCTTGGA AGAAGATAGT 
TGCCAAGAAG AGCAGAGTGT GGATTCAGAC TTAGGTGAAG CAGCATCCGA CCATGTGAGT 
GAAACAAGCC TCTCTGAAGA CTGTGTGGGG CTGTCCTCTC AGAGTGATAT CTTAACCACT 
CAGCAGAGGG ATACCATGCA AGATAACCTT CTAAAGCTCC AGCAGGAAAT GGCCGAGCTG 
GAAGCTGTGT TAGAGCAGCA CGGGAGCCAG CCTTCCCACA GCTCTGCTTC CCTCACAGCT 
GATTCTCGTG GCCCAGAGCA CCTGCTAAAT CTAGAGCAAG ACACATCAGA AAGAGCAATA 
TTAACTTCAG AGAAAAGTAG AGACTGTTCT AGAAGCCAGA CTCCTGAAAG CCTGTCTGCT 
GACAAGTTTC CAGTATCTCT GGATAGTTCT ACCAATAAAA ATAAAGAACC AGGAATGGAA 
AGATCTTCCC CTTCTAAATC CCAGTTGCCG GGTAATAGGT GGTATATGCA CAGCTCAAGG 
AGTCTTCAGG ACAGAAACTG CCCATCTCAA AAGAAGCGCA TTAATGTTGC TGACATGCAG 
GAGCAACAAC TGGCTAAGTG TGAGGCCCAA GATTTAATGG GGTCTTATTT GCCGAGACAA 
GATCAAGAGG GAACCCCTTA CCTGAAATCT GGAATCAGCC TCTTCTCTCA TGAACCCGAG 
TCTGATCCCT CTGAAGACAG AGCCCCAGAG CCAGCTCATG TTCACAGCAT GCCACCCACA 
GCCTCTGCAC TGAAATTATC CCAATTTCGA GTAGAAGAAT CTACCAAGAA TCCAGCTGCT 
GCTCACATTA CTAATACTAC AGGGTGTAAC CTGAGGGAAG AAAGTGTGAG CAAAGAGAAG 
CCAGAAGTGT TATCTTCAAC AGAAAGAAGC AACAAAAGAC TTTCTATGGT GGCATCAGGC 
TTGACCCCAA AAGAACTCAT GCTTGTGCAG AAATTTGCCA GAAAACATCA CGTCACTTTA 
ACTAATCTAA TTACTGAAGA GACTACTCAT GTCATTATGA AAACAGATCC TGAGTTTGTG 
TGTGAACGGA CACTGAAATA TTTTCTGGGA ATTGCAGGAG GAAAATGGGT AGTTAGCTAT 
TTTTGGGTCA TCCAGTCTAT TAAAGAAAGA AAGATGCTGG ATGAGCATGA TTTTGAAGTC 
AGAGGAGATG TTGTCAATGG AAGAAACCAT CAAGGCCCAA AACGAGCAAG AGAATCCCGG 
GACAAGAAGA TCTTCAAGGG CCTGGAAATC TGTTGCTATG GACCTTTTAC CAATATGCCC 
ACAGATCAAC TAGAATGGAT GGTGCAGCTG TGTGGGGCTT CTGTGGTGAA GGAGCCCTCA 
TCATTCACCC CAGGCCAGGG TACTCACCCA GTTGTGGTTG TGCAGCCGGA TGCCTGGACA 
GAGGATGCTG GCTTCCATGT GATCGGGCAG ATGTGTGAAG CACCTGTGGT AACCCGAGAG 
TGGGTACTGG ACAGTGTGGC CCTTTACCAG TGCCAGGAGC TGGACACCTA CCTGGTCCCC 
CAGGTCGCCC AGAGCTGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014965016.1
CDS	214..5817
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLNIKDLITTQEHAAEMHAAETHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQESTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESEETASSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQTPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015109530.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015109530.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAATATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATGCAG CTGAGACGCA TCCAGAAAAG TATCAGGGTA TTTCTGTTTC AGACTTTCAC 
GTGGAGCCAT GTGGCACAGA TACTCATGCC AGCTCATTAC AGCATGAGAA CAGCAGTTTA 
TTGCTCACTG AAAACAGACT GAATGTAGAA AAGGCTGAAT TCTGTAATAA AAGCAAACAG 
CCTGTCTTAG TAAAGAGCCA ACAGAGCAGA TGGGCTGAAA GTAAGGGCAC ATGTAAGGAT 
AGGCAGATTC CCAGCACTGG GAAAAAGATA GTTCTGAATA CTGATCCCCT GTATGGAAGA 
AAAGAACTGT GTAAGCAGAA ACCTGCATGC TCTGACAGTC CTGGAGATTC CCAAGATGTT 
CCTTGGGTAA CCCTGAATAA TAGCATACAG AAAGTTAATG ACTGGTTTTC CAGAAGTGAT 
GAAATATTAA CTTCTGACGA CTCGTGCGAT GGGGGGTCTG AATCAAATAA TGAAGTAGCT 
GGTGCAGTGG AAATTCCAAA TAACGTTGAT GGATATTCAG GTTCTTCAGA GAAAATTGAC 
TTAATGGCCA GTGATCCTCA TGGTACTTTA ATACATGAAA GAGTCCACTC CAAACCAGTA 
GAGAGTAATA TTGAAGATAA AATATTTGGG AAAACCTATC GGAGGAAGTC GAGTCTCCCT 
AACTTCAACC ACATAGCTGA AGATCTAATT ATAGGTGCAT TTACTGTAGA ACCTCAGAAA 
ACACAAGAGC AGCCCCTCAC AAATAAGCTA AAATGTAAAA GGAGAGGTAC ATCAGGCCTT 
CAGCCTGAGG ATTTTATCAA GAAAGGTGAT TTGACGATTG TTCCAAAGAC TCCTGAAAAG 
ATGATTGAGA GAACTGACCA AACAGAGCAG AAACATCACG GGATAAATAT TACTAGTGAT 
GGTCATGAGA ATAAAACAAA ACGTGATGAT GTTCAGAAAG AGCAAAACGC TAACCCAACA 
GAATCATTGG AAAACGAATC TGTTTTCAGA ACTAAGGCTG AACCTATAAA CACCAGTATA 
AGCAATATGG AACTAGAATT GAATGTCCGC AGTTCAAAAG CACCTAAGAA TAGGCTGAAG 
AGGAAATCCT CTACCAGGAA AATTCCTGAA CTTGAACTAG TAGTCAGTAG AAATCCAAGT 
CCACCTAATC ATACTGAGCT ACTAATTGAT AGCAGTTCTA GCAATGAAGA GATGAAGAAA 
AAGCATTCTA GCCAAATGCC AGTCAGGCAC AGCCAAAAGC TTCAACTCAT GGGAGATAAA 
GAACCTACAG CTGGAGCCAA GAAGAGTAAC AAGACATATG AACAAATAAA TAAAAGACTT 
GCTAGTGATG CTTTTCCAGA ACTAAATTTT GCAAACACAC CTGGTTATTT TACTAACTGT 
TCTAGTAAAC CTGAAGAGTT TGTTCATCCT AGCCTTCAAA GAGAGGAGAA TCTAGGAACA 
ATTCAAGTGT TGAATAATAC CAAAGACCCC AAAGATCTGA TATTAAGAGG AGGAAAAGCT 
TTGCAAATGG AAAGATCTGT AGAGAATACC AATATTTCCT TGGTTCCTGA TACTGATTAT 
AGCACTCAGG ATAGTATCTC ATTACTAGAA GCTAAAACCC CAGAGAAGGC AAAGACTGCA 
CCAAATCCAT GTGTGAGTCT GTGTGCAGCA ATCGAAAACC CCAAGGAACT TATCCATAGG 
GATTTTAAAG ATAACAAAAA CAACACAGAG GGCTTTCAGG GTCTCCTGGG ACATGACGTT 
AACTATGTCA TTCAGGAGAC AAGAGGAGAA ATGGAAGACA GTGAACTTGA TACACAGTAT 
TTGCAGAATA CGTTCAAGGT TTCAAAGCGT CAGACATTTG CTCTGTTTTC CAATCCAGGA 
AATCCACAAA AGGAATGTGC CACAGTCTTT GCCCACTCGG GGTCCTTAAA GGATCAAAGT 
CCAAGAGACC CCCTCAAATG CAGACAAAAA GAAGACAGTC GGGGAAAGAG TGAGTCTAAA 
AGCCAGCATG TGCAGGCCGT TTGTACAACA GTGCACTTTC CTGTGGCTGG TCAGAAAGAT 
AAGACGCCGG GTGACGATGC CAAATGTAGT GCAAAAGAAG TCACTAGGGT TTGTCAGTCA 
TTACAATTGA GAGGCCACAA AACTGAACTT GTTTTTGCTA ATAAGCAAGG GGTTTCAGAA 
AAACCAAATC TAACACCATC ACTTTTTCCC ATCAAGTCAT CTGTTAAAAC CGTATGTAAG 
AAAAGCCCAT CAGAGAAATT TGAGGAACCT GTAATGTCAC CTGAAAGAAC ATTGGGAAGT 
GAGAGCATCA TTCAAAGTGC AGTGAGCACA ATCAGCCAAA ATGACATTCA AGAAAGCACT 
TTTAAAGAAG TCAACTCAAG CAGTGTTAAT GAAGTAGGTT CCAGTACTAA TGAAGTAGGC 
TCTAGTATTA ATGAAGTAGG TTCCAGTGGT GAAAACATTC AAGCAGAACC AGGTAGAAAC 
AGAGAACCTA AATTAAGAGC TTTACTCAGA TTAGGTCTTA TGCAACCTGA AGTCTATAAG 
CAAAGCCTTC CTGTAAGTAA CTGTCACCAT CCTGAAATAA AAAGGCAAGG AGAAAATGAG 
GACATGCTTC AGGCTGTTAA GGCAGATTTC TCCCCATGTC TAATTTCAGA TAACCTCGAA 
CAACCTATGG GAAGCTGTCA TGCTTCTCAG GTTTGTTCTG AGACACCTGA CAACTTGTTA 
AATGACGACG AAATAAAAGA AAATAGCCAC TTTGCTGAAA GTGACATTAA GGAAAGATCT 
GCTGTTTTTA GTGAAAGTGT CCAAAAAGGA GAATTCAGAG GGAGCCCTGG CCCTTTCACC 
CATACACATT TGGCTCAGGG TCACCAAAGA GGGGCTGGGA AACTAGAGTC AGAAGAGACT 
GCGTCTAGTG AGGATGAAGA GCTTCCCTGC TTCCAACAAT TACTGTTTGG TAAAGTAACC 
GATACACTGT CTCCATCTAC TGGATGGAAC ACTATTGCCA CTGAGGGTCT GTCTAAGGAA 
ACAGAGGAGA ACCTAGAATC ATTGAAGAGT GGCTTAAATG ACTGCAGTGG CCAGGGAACA 
TCAGCCAAGG TATCCCAGGA ACATCACCTT AATGAGGAAG TGAGATGTTC TGGTAGCTTA 
TTTTCCTCAC AGTACAGTGC AGTGGAAGAC TTGACCACTA ATACAAACAC CCAGGATCCT 
TTCTTGATGT TTGAGCGTCC TTCCAAACAA GTGTATCAGT CTGAAAGCGA GGAAGTTCTG 
AGTGACAAGG AATTTGTTTC AGATGATGAA GAAAGGGAAA CTGGCTTGGA AGAAGATAGT 
TGCCAAGAAG AGCAGAGTGT GGATTCAGAC TTAGGTGAAG CAGCATCCGA CCATGTGAGT 
GAAACAAGCC TCTCTGAAGA CTGTGTGGGG CTGTCCTCTC AGAGTGATAT CTTAACCACT 
CAGCAGAGGG ATACCATGCA AGATAACCTT CTAAAGCTCC AGCAGGAAAT GGCCGAGCTG 
GAAGCTGTGT TAGAGCAGCA CGGGAGCCAG CCTTCCCACA GCTCTGCTTC CCTCACAGCT 
GATTCTCGTG GCCCAGAGCA CCTGCTAAAT CTAGAGCAAG ACACATCAGA AAGAGCAATA 
TTAACTTCAG AGAAAAGTAG AGACTGTTCT AGAAGCCAGA CTCCTGAAAG CCTGTCTGCT 
GACAAGTTTC CAGTATCTCT GGATAGTTCT ACCAATAAAA ATAAAGAACC AGGAATGGAA 
AGATCTTCCC CTTCTAAATC CCAGTTGCCG GGTAATAGGT GGTATATGCA CAGCTCAAGG 
AGTCTTCAGG ACAGAAACTG CCCATCTCAA AAGAAGCGCA TTAATGTTGC TGACATGCAG 
GAGCAACAAC TGGCTAAGTG TGAGGCCCAA GATTTAATGG GGTCTTATTT GCCGAGACAA 
GATCAAGAGG GAACCCCTTA CCTGAAATCT GGAATCAGCC TCTTCTCTCA TGAACCCGAG 
TCTGATCCCT CTGAAGACAG AGCCCCAGAG CCAGCTCATG TTCACAGCAT GCCACCCACA 
GCCTCTGCAC TGAAATTATC CCAATTTCGA GTAGAAGAAT CTACCAAGAA TCCAGCTGCT 
GCTCACATTA CTAATACTAC AGGGTGTAAC CTGAGGGAAG AAAGTGTGAG CAAAGAGAAG 
CCAGAAGTGT TATCTTCAAC AGAAAGAAGC AACAAAAGAC TTTCTATGGT GGCATCAGGC 
TTGACCCCAA AAGAACTCAT GCTTGTGCAG AAATTTGCCA GAAAACATCA CGTCACTTTA 
ACTAATCTAA TTACTGAAGA GACTACTCAT GTCATTATGA AAACAGATCC TGAGTTTGTG 
TGTGAACGGA CACTGAAATA TTTTCTGGGA ATTGCAGGAG GAAAATGGGT AGTTAGCTAT 
TTTTGGGTCA TCCAGTCTAT TAAAGAAAGA AAGATGCTGG ATGAGCATGA TTTTGAAGTC 
AGAGGAGATG TTGTCAATGG AAGAAACCAT CAAGGCCCAA AACGAGCAAG AGAATCCCGG 
GACAAGAAGA TCTTCAAGGG CCTGGAAATC TGTTGCTATG GACCTTTTAC CAATATGCCC 
ACAGATCAAC TAGAATGGAT GGTGCAGCTG TGTGGGGCTT CTGTGGTGAA GGAGCCCTCA 
TCATTCACCC CAGGCCAGGG TACTCACCCA GTTGTGGTTG TGCAGCCGGA TGCCTGGACA 
GAGGATGCTG GCTTCCATGT GATCGGGCAG ATGTGTGAAG CACCTGTGGT AACCCGAGAG 
TGGGTACTGG ACAGTGTGGC CCTTTACCAG TGCCAGGAGC TGGACACCTA CCTGGTCCCC 
CAGGTCGCCC AGAGCTGCTG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015109533.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014965019.1 Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X4, mRNA.

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CloneID	OOv27529
Clone ID Related Accession (Same CDS sequence)	XM_015109533.1
Accession Version	XM_015109533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	breast cancer type 1 susceptibility protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GGAAACCAGT CTTACTGTCA AACTCTCTAA CCTTGGAATT 
GTGAGATCTC TGAGGACAAA GCAGCAGACA CAATCTCAGA ATAAGTCTGT CTACATTGAA 
TTGGGATCTG ATTCTTCTGA AGACACAGTT AATAAGGCCA GTTATCTCAG TGTGGGAGAC 
CATGAATTGT TAGAAATCAC CCCTCAAGGA GCCAAGGCCA AGACCAATTT GAATCCTGCA 
AAAAAGGCTG CTTGTGAGTT TTCTGAGAAG GACATAACAA ATACTGAACA TCATCAACTC 
AATATTAAAG ATCTGATCAC CACCCAGGAG CATGCAGCTG AGATGCATGC AGCTGAGACG 
CATGCAGCTG AGACGCATCC AGAAAAGTAT CAGGGTATTT CTGTTTCAGA CTTTCACGTG 
GAGCCATGTG GCACAGATAC TCATGCCAGC TCATTACAGC ATGAGAACAG CAGTTTATTG 
CTCACTGAAA ACAGACTGAA TGTAGAAAAG GCTGAATTCT GTAATAAAAG CAAACAGCCT 
GTCTTAGTAA AGAGCCAACA GAGCAGATGG GCTGAAAGTA AGGGCACATG TAAGGATAGG 
CAGATTCCCA GCACTGGGAA AAAGATAGTT CTGAATACTG ATCCCCTGTA TGGAAGAAAA 
GAACTGTGTA AGCAGAAACC TGCATGCTCT GACAGTCCTG GAGATTCCCA AGATGTTCCT 
TGGGTAACCC TGAATAATAG CATACAGAAA GTTAATGACT GGTTTTCCAG AAGTGATGAA 
ATATTAACTT CTGACGACTC GTGCGATGGG GGGTCTGAAT CAAATAATGA AGTAGCTGGT 
GCAGTGGAAA TTCCAAATAA CGTTGATGGA TATTCAGGTT CTTCAGAGAA AATTGACTTA 
ATGGCCAGTG ATCCTCATGG TACTTTAATA CATGAAAGAG TCCACTCCAA ACCAGTAGAG 
AGTAATATTG AAGATAAAAT ATTTGGGAAA ACCTATCGGA GGAAGTCGAG TCTCCCTAAC 
TTCAACCACA TAGCTGAAGA TCTAATTATA GGTGCATTTA CTGTAGAACC TCAGAAAACA 
CAAGAGCAGC CCCTCACAAA TAAGCTAAAA TGTAAAAGGA GAGGTACATC AGGCCTTCAG 
CCTGAGGATT TTATCAAGAA AGGTGATTTG ACGATTGTTC CAAAGACTCC TGAAAAGATG 
ATTGAGAGAA CTGACCAAAC AGAGCAGAAA CATCACGGGA TAAATATTAC TAGTGATGGT 
CATGAGAATA AAACAAAACG TGATGATGTT CAGAAAGAGC AAAACGCTAA CCCAACAGAA 
TCATTGGAAA ACGAATCTGT TTTCAGAACT AAGGCTGAAC CTATAAACAC CAGTATAAGC 
AATATGGAAC TAGAATTGAA TGTCCGCAGT TCAAAAGCAC CTAAGAATAG GCTGAAGAGG 
AAATCCTCTA CCAGGAAAAT TCCTGAACTT GAACTAGTAG TCAGTAGAAA TCCAAGTCCA 
CCTAATCATA CTGAGCTACT AATTGATAGC AGTTCTAGCA ATGAAGAGAT GAAGAAAAAG 
CATTCTAGCC AAATGCCAGT CAGGCACAGC CAAAAGCTTC AACTCATGGG AGATAAAGAA 
CCTACAGCTG GAGCCAAGAA GAGTAACAAG ACATATGAAC AAATAAATAA AAGACTTGCT 
AGTGATGCTT TTCCAGAACT AAATTTTGCA AACACACCTG GTTATTTTAC TAACTGTTCT 
AGTAAACCTG AAGAGTTTGT TCATCCTAGC CTTCAAAGAG AGGAGAATCT AGGAACAATT 
CAAGTGTTGA ATAATACCAA AGACCCCAAA GATCTGATAT TAAGAGGAGG AAAAGCTTTG 
CAAATGGAAA GATCTGTAGA GAATACCAAT ATTTCCTTGG TTCCTGATAC TGATTATAGC 
ACTCAGGATA GTATCTCATT ACTAGAAGCT AAAACCCCAG AGAAGGCAAA GACTGCACCA 
AATCCATGTG TGAGTCTGTG TGCAGCAATC GAAAACCCCA AGGAACTTAT CCATAGGGAT 
TTTAAAGATA ACAAAAACAA CACAGAGGGC TTTCAGGGTC TCCTGGGACA TGACGTTAAC 
TATGTCATTC AGGAGACAAG AGGAGAAATG GAAGACAGTG AACTTGATAC ACAGTATTTG 
CAGAATACGT TCAAGGTTTC AAAGCGTCAG ACATTTGCTC TGTTTTCCAA TCCAGGAAAT 
CCACAAAAGG AATGTGCCAC AGTCTTTGCC CACTCGGGGT CCTTAAAGGA TCAAAGTCCA 
AGAGACCCCC TCAAATGCAG ACAAAAAGAA GACAGTCGGG GAAAGAGTGA GTCTAAAAGC 
CAGCATGTGC AGGCCGTTTG TACAACAGTG CACTTTCCTG TGGCTGGTCA GAAAGATAAG 
ACGCCGGGTG ACGATGCCAA ATGTAGTGCA AAAGAAGTCA CTAGGGTTTG TCAGTCATTA 
CAATTGAGAG GCCACAAAAC TGAACTTGTT TTTGCTAATA AGCAAGGGGT TTCAGAAAAA 
CCAAATCTAA CACCATCACT TTTTCCCATC AAGTCATCTG TTAAAACCGT ATGTAAGAAA 
AGCCCATCAG AGAAATTTGA GGAACCTGTA ATGTCACCTG AAAGAACATT GGGAAGTGAG 
AGCATCATTC AAAGTGCAGT GAGCACAATC AGCCAAAATG ACATTCAAGA AAGCACTTTT 
AAAGAAGTCA ACTCAAGCAG TGTTAATGAA GTAGGTTCCA GTACTAATGA AGTAGGCTCT 
AGTATTAATG AAGTAGGTTC CAGTGGTGAA AACATTCAAG CAGAACCAGG TAGAAACAGA 
GAACCTAAAT TAAGAGCTTT ACTCAGATTA GGTCTTATGC AACCTGAAGT CTATAAGCAA 
AGCCTTCCTG TAAGTAACTG TCACCATCCT GAAATAAAAA GGCAAGGAGA AAATGAGGAC 
ATGCTTCAGG CTGTTAAGGC AGATTTCTCC CCATGTCTAA TTTCAGATAA CCTCGAACAA 
CCTATGGGAA GCTGTCATGC TTCTCAGGTT TGTTCTGAGA CACCTGACAA CTTGTTAAAT 
GACGACGAAA TAAAAGAAAA TAGCCACTTT GCTGAAAGTG ACATTAAGGA AAGATCTGCT 
GTTTTTAGTG AAAGTGTCCA AAAAGGAGAA TTCAGAGGGA GCCCTGGCCC TTTCACCCAT 
ACACATTTGG CTCAGGGTCA CCAAAGAGGG GCTGGGAAAC TAGAGTCAGA AGAGACTGCG 
TCTAGTGAGG ATGAAGAGCT TCCCTGCTTC CAACAATTAC TGTTTGGTAA AGTAACCGAT 
ACACTGTCTC CATCTACTGG ATGGAACACT ATTGCCACTG AGGGTCTGTC TAAGGAAACA 
GAGGAGAACC TAGAATCATT GAAGAGTGGC TTAAATGACT GCAGTGGCCA GGGAACATCA 
GCCAAGGTAT CCCAGGAACA TCACCTTAAT GAGGAAGTGA GATGTTCTGG TAGCTTATTT 
TCCTCACAGT ACAGTGCAGT GGAAGACTTG ACCACTAATA CAAACACCCA GGATCCTTTC 
TTGATGTTTG AGCGTCCTTC CAAACAAGTG TATCAGTCTG AAAGCGAGGA AGTTCTGAGT 
GACAAGGAAT TTGTTTCAGA TGATGAAGAA AGGGAAACTG GCTTGGAAGA AGATAGTTGC 
CAAGAAGAGC AGAGTGTGGA TTCAGACTTA GGTGAAGCAG CATCCGACCA TGTGAGTGAA 
ACAAGCCTCT CTGAAGACTG TGTGGGGCTG TCCTCTCAGA GTGATATCTT AACCACTCAG 
CAGAGGGATA CCATGCAAGA TAACCTTCTA AAGCTCCAGC AGGAAATGGC CGAGCTGGAA 
GCTGTGTTAG AGCAGCACGG GAGCCAGCCT TCCCACAGCT CTGCTTCCCT CACAGCTGAT 
TCTCGTGGCC CAGAGCACCT GCTAAATCTA GAGCAAGACA CATCAGAAAG AGCAATATTA 
ACTTCAGAGA AAAGTAGAGA CTGTTCTAGA AGCCAGACTC CTGAAAGCCT GTCTGCTGAC 
AAGTTTCCAG TATCTCTGGA TAGTTCTACC AATAAAAATA AAGAACCAGG AATGGAAAGA 
TCTTCCCCTT CTAAATCCCA GTTGCCGGGT AATAGGTGGT ATATGCACAG CTCAAGGAGT 
CTTCAGGACA GAAACTGCCC ATCTCAAAAG AAGCGCATTA ATGTTGCTGA CATGCAGGAG 
CAACAACTGG CTAAGTGTGA GGCCCAAGAT TTAATGGGGT CTTATTTGCC GAGACAAGAT 
CAAGAGGGAA CCCCTTACCT GAAATCTGGA ATCAGCCTCT TCTCTCATGA ACCCGAGTCT 
GATCCCTCTG AAGACAGAGC CCCAGAGCCA GCTCATGTTC ACAGCATGCC ACCCACAGCC 
TCTGCACTGA AATTATCCCA ATTTCGAGTA GAAGAATCTA CCAAGAATCC AGCTGCTGCT 
CACATTACTA ATACTACAGG GTGTAACCTG AGGGAAGAAA GTGTGAGCAA AGAGAAGCCA 
GAAGTGTTAT CTTCAACAGA AAGAAGCAAC AAAAGACTTT CTATGGTGGC ATCAGGCTTG 
ACCCCAAAAG AACTCATGCT TGTGCAGAAA TTTGCCAGAA AACATCACGT CACTTTAACT 
AATCTAATTA CTGAAGAGAC TACTCATGTC ATTATGAAAA CAGATCCTGA GTTTGTGTGT 
GAACGGACAC TGAAATATTT TCTGGGAATT GCAGGAGGAA AATGGGTAGT TAGCTATTTT 
TGGGTCATCC AGTCTATTAA AGAAAGAAAG ATGCTGGATG AGCATGATTT TGAAGTCAGA 
GGAGATGTTG TCAATGGAAG AAACCATCAA GGCCCAAAAC GAGCAAGAGA ATCCCGGGAC 
AAGAAGATCT TCAAGGGCCT GGAAATCTGT TGCTATGGAC CTTTTACCAA TATGCCCACA 
GATCAACTAG AATGGATGGT GCAGCTGTGT GGGGCTTCTG TGGTGAAGGA GCCCTCATCA 
TTCACCCCAG GCCAGGGTAC TCACCCAGTT GTGGTTGTGC AGCCGGATGC CTGGACAGAG 
GATGCTGGCT TCCATGTGAT CGGGCAGATG TGTGAAGCAC CTGTGGTAAC CCGAGAGTGG 
GTACTGGACA GTGTGGCCCT TTACCAGTGC CAGGAGCTGG ACACCTACCT GGTCCCCCAG 
GTCGCCCAGA GCTGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014965019.1
CDS	215..5815
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLNIKDLITTQEHAAEMHAAETHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQESTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESEETASSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQTPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015109533.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015109533.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GGAAACCAGT CTTACTGTCA AACTCTCTAA CCTTGGAATT 
GTGAGATCTC TGAGGACAAA GCAGCAGACA CAATCTCAGA ATAAGTCTGT CTACATTGAA 
TTGGGATCTG ATTCTTCTGA AGACACAGTT AATAAGGCCA GTTATCTCAG TGTGGGAGAC 
CATGAATTGT TAGAAATCAC CCCTCAAGGA GCCAAGGCCA AGACCAATTT GAATCCTGCA 
AAAAAGGCTG CTTGTGAGTT TTCTGAGAAG GACATAACAA ATACTGAACA TCATCAACTC 
AATATTAAAG ATCTGATCAC CACCCAGGAG CATGCAGCTG AGATGCATGC AGCTGAGACG 
CATGCAGCTG AGACGCATCC AGAAAAGTAT CAGGGTATTT CTGTTTCAGA CTTTCACGTG 
GAGCCATGTG GCACAGATAC TCATGCCAGC TCATTACAGC ATGAGAACAG CAGTTTATTG 
CTCACTGAAA ACAGACTGAA TGTAGAAAAG GCTGAATTCT GTAATAAAAG CAAACAGCCT 
GTCTTAGTAA AGAGCCAACA GAGCAGATGG GCTGAAAGTA AGGGCACATG TAAGGATAGG 
CAGATTCCCA GCACTGGGAA AAAGATAGTT CTGAATACTG ATCCCCTGTA TGGAAGAAAA 
GAACTGTGTA AGCAGAAACC TGCATGCTCT GACAGTCCTG GAGATTCCCA AGATGTTCCT 
TGGGTAACCC TGAATAATAG CATACAGAAA GTTAATGACT GGTTTTCCAG AAGTGATGAA 
ATATTAACTT CTGACGACTC GTGCGATGGG GGGTCTGAAT CAAATAATGA AGTAGCTGGT 
GCAGTGGAAA TTCCAAATAA CGTTGATGGA TATTCAGGTT CTTCAGAGAA AATTGACTTA 
ATGGCCAGTG ATCCTCATGG TACTTTAATA CATGAAAGAG TCCACTCCAA ACCAGTAGAG 
AGTAATATTG AAGATAAAAT ATTTGGGAAA ACCTATCGGA GGAAGTCGAG TCTCCCTAAC 
TTCAACCACA TAGCTGAAGA TCTAATTATA GGTGCATTTA CTGTAGAACC TCAGAAAACA 
CAAGAGCAGC CCCTCACAAA TAAGCTAAAA TGTAAAAGGA GAGGTACATC AGGCCTTCAG 
CCTGAGGATT TTATCAAGAA AGGTGATTTG ACGATTGTTC CAAAGACTCC TGAAAAGATG 
ATTGAGAGAA CTGACCAAAC AGAGCAGAAA CATCACGGGA TAAATATTAC TAGTGATGGT 
CATGAGAATA AAACAAAACG TGATGATGTT CAGAAAGAGC AAAACGCTAA CCCAACAGAA 
TCATTGGAAA ACGAATCTGT TTTCAGAACT AAGGCTGAAC CTATAAACAC CAGTATAAGC 
AATATGGAAC TAGAATTGAA TGTCCGCAGT TCAAAAGCAC CTAAGAATAG GCTGAAGAGG 
AAATCCTCTA CCAGGAAAAT TCCTGAACTT GAACTAGTAG TCAGTAGAAA TCCAAGTCCA 
CCTAATCATA CTGAGCTACT AATTGATAGC AGTTCTAGCA ATGAAGAGAT GAAGAAAAAG 
CATTCTAGCC AAATGCCAGT CAGGCACAGC CAAAAGCTTC AACTCATGGG AGATAAAGAA 
CCTACAGCTG GAGCCAAGAA GAGTAACAAG ACATATGAAC AAATAAATAA AAGACTTGCT 
AGTGATGCTT TTCCAGAACT AAATTTTGCA AACACACCTG GTTATTTTAC TAACTGTTCT 
AGTAAACCTG AAGAGTTTGT TCATCCTAGC CTTCAAAGAG AGGAGAATCT AGGAACAATT 
CAAGTGTTGA ATAATACCAA AGACCCCAAA GATCTGATAT TAAGAGGAGG AAAAGCTTTG 
CAAATGGAAA GATCTGTAGA GAATACCAAT ATTTCCTTGG TTCCTGATAC TGATTATAGC 
ACTCAGGATA GTATCTCATT ACTAGAAGCT AAAACCCCAG AGAAGGCAAA GACTGCACCA 
AATCCATGTG TGAGTCTGTG TGCAGCAATC GAAAACCCCA AGGAACTTAT CCATAGGGAT 
TTTAAAGATA ACAAAAACAA CACAGAGGGC TTTCAGGGTC TCCTGGGACA TGACGTTAAC 
TATGTCATTC AGGAGACAAG AGGAGAAATG GAAGACAGTG AACTTGATAC ACAGTATTTG 
CAGAATACGT TCAAGGTTTC AAAGCGTCAG ACATTTGCTC TGTTTTCCAA TCCAGGAAAT 
CCACAAAAGG AATGTGCCAC AGTCTTTGCC CACTCGGGGT CCTTAAAGGA TCAAAGTCCA 
AGAGACCCCC TCAAATGCAG ACAAAAAGAA GACAGTCGGG GAAAGAGTGA GTCTAAAAGC 
CAGCATGTGC AGGCCGTTTG TACAACAGTG CACTTTCCTG TGGCTGGTCA GAAAGATAAG 
ACGCCGGGTG ACGATGCCAA ATGTAGTGCA AAAGAAGTCA CTAGGGTTTG TCAGTCATTA 
CAATTGAGAG GCCACAAAAC TGAACTTGTT TTTGCTAATA AGCAAGGGGT TTCAGAAAAA 
CCAAATCTAA CACCATCACT TTTTCCCATC AAGTCATCTG TTAAAACCGT ATGTAAGAAA 
AGCCCATCAG AGAAATTTGA GGAACCTGTA ATGTCACCTG AAAGAACATT GGGAAGTGAG 
AGCATCATTC AAAGTGCAGT GAGCACAATC AGCCAAAATG ACATTCAAGA AAGCACTTTT 
AAAGAAGTCA ACTCAAGCAG TGTTAATGAA GTAGGTTCCA GTACTAATGA AGTAGGCTCT 
AGTATTAATG AAGTAGGTTC CAGTGGTGAA AACATTCAAG CAGAACCAGG TAGAAACAGA 
GAACCTAAAT TAAGAGCTTT ACTCAGATTA GGTCTTATGC AACCTGAAGT CTATAAGCAA 
AGCCTTCCTG TAAGTAACTG TCACCATCCT GAAATAAAAA GGCAAGGAGA AAATGAGGAC 
ATGCTTCAGG CTGTTAAGGC AGATTTCTCC CCATGTCTAA TTTCAGATAA CCTCGAACAA 
CCTATGGGAA GCTGTCATGC TTCTCAGGTT TGTTCTGAGA CACCTGACAA CTTGTTAAAT 
GACGACGAAA TAAAAGAAAA TAGCCACTTT GCTGAAAGTG ACATTAAGGA AAGATCTGCT 
GTTTTTAGTG AAAGTGTCCA AAAAGGAGAA TTCAGAGGGA GCCCTGGCCC TTTCACCCAT 
ACACATTTGG CTCAGGGTCA CCAAAGAGGG GCTGGGAAAC TAGAGTCAGA AGAGACTGCG 
TCTAGTGAGG ATGAAGAGCT TCCCTGCTTC CAACAATTAC TGTTTGGTAA AGTAACCGAT 
ACACTGTCTC CATCTACTGG ATGGAACACT ATTGCCACTG AGGGTCTGTC TAAGGAAACA 
GAGGAGAACC TAGAATCATT GAAGAGTGGC TTAAATGACT GCAGTGGCCA GGGAACATCA 
GCCAAGGTAT CCCAGGAACA TCACCTTAAT GAGGAAGTGA GATGTTCTGG TAGCTTATTT 
TCCTCACAGT ACAGTGCAGT GGAAGACTTG ACCACTAATA CAAACACCCA GGATCCTTTC 
TTGATGTTTG AGCGTCCTTC CAAACAAGTG TATCAGTCTG AAAGCGAGGA AGTTCTGAGT 
GACAAGGAAT TTGTTTCAGA TGATGAAGAA AGGGAAACTG GCTTGGAAGA AGATAGTTGC 
CAAGAAGAGC AGAGTGTGGA TTCAGACTTA GGTGAAGCAG CATCCGACCA TGTGAGTGAA 
ACAAGCCTCT CTGAAGACTG TGTGGGGCTG TCCTCTCAGA GTGATATCTT AACCACTCAG 
CAGAGGGATA CCATGCAAGA TAACCTTCTA AAGCTCCAGC AGGAAATGGC CGAGCTGGAA 
GCTGTGTTAG AGCAGCACGG GAGCCAGCCT TCCCACAGCT CTGCTTCCCT CACAGCTGAT 
TCTCGTGGCC CAGAGCACCT GCTAAATCTA GAGCAAGACA CATCAGAAAG AGCAATATTA 
ACTTCAGAGA AAAGTAGAGA CTGTTCTAGA AGCCAGACTC CTGAAAGCCT GTCTGCTGAC 
AAGTTTCCAG TATCTCTGGA TAGTTCTACC AATAAAAATA AAGAACCAGG AATGGAAAGA 
TCTTCCCCTT CTAAATCCCA GTTGCCGGGT AATAGGTGGT ATATGCACAG CTCAAGGAGT 
CTTCAGGACA GAAACTGCCC ATCTCAAAAG AAGCGCATTA ATGTTGCTGA CATGCAGGAG 
CAACAACTGG CTAAGTGTGA GGCCCAAGAT TTAATGGGGT CTTATTTGCC GAGACAAGAT 
CAAGAGGGAA CCCCTTACCT GAAATCTGGA ATCAGCCTCT TCTCTCATGA ACCCGAGTCT 
GATCCCTCTG AAGACAGAGC CCCAGAGCCA GCTCATGTTC ACAGCATGCC ACCCACAGCC 
TCTGCACTGA AATTATCCCA ATTTCGAGTA GAAGAATCTA CCAAGAATCC AGCTGCTGCT 
CACATTACTA ATACTACAGG GTGTAACCTG AGGGAAGAAA GTGTGAGCAA AGAGAAGCCA 
GAAGTGTTAT CTTCAACAGA AAGAAGCAAC AAAAGACTTT CTATGGTGGC ATCAGGCTTG 
ACCCCAAAAG AACTCATGCT TGTGCAGAAA TTTGCCAGAA AACATCACGT CACTTTAACT 
AATCTAATTA CTGAAGAGAC TACTCATGTC ATTATGAAAA CAGATCCTGA GTTTGTGTGT 
GAACGGACAC TGAAATATTT TCTGGGAATT GCAGGAGGAA AATGGGTAGT TAGCTATTTT 
TGGGTCATCC AGTCTATTAA AGAAAGAAAG ATGCTGGATG AGCATGATTT TGAAGTCAGA 
GGAGATGTTG TCAATGGAAG AAACCATCAA GGCCCAAAAC GAGCAAGAGA ATCCCGGGAC 
AAGAAGATCT TCAAGGGCCT GGAAATCTGT TGCTATGGAC CTTTTACCAA TATGCCCACA 
GATCAACTAG AATGGATGGT GCAGCTGTGT GGGGCTTCTG TGGTGAAGGA GCCCTCATCA 
TTCACCCCAG GCCAGGGTAC TCACCCAGTT GTGGTTGTGC AGCCGGATGC CTGGACAGAG 
GATGCTGGCT TCCATGTGAT CGGGCAGATG TGTGAAGCAC CTGTGGTAAC CCGAGAGTGG 
GTACTGGACA GTGTGGCCCT TTACCAGTGC CAGGAGCTGG ACACCTACCT GGTCCCCCAG 
GTCGCCCAGA GCTGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_012152391.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012007781.1 Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X5, mRNA.

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CloneID	OOv27530
Clone ID Related Accession (Same CDS sequence)	XM_012152391.2 , XM_012152392.1
Accession Version	XM_012152391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	breast cancer type 1 susceptibility protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012152391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAAT GTCCATTGTG TAAGAATGAT 
ATAACCAAAA GAAGCCTACA AGAAAGTACA AGATTTAGTC AACTTGTTGA AGAGCTGTTG 
AAGATCATTC ATGCTTTTGA GCTTGACACA GGATTGCAGT TTGCAAACAG CTATAACTTT 
TCAAGAAAGG AAAATAACTC TCCTGAGCAT CTGAAGGAGG AAGTTTCTAT AATCCAAAGT 
ATGGGCTACC GGAACCGGGC CAAAAGACTT TGGCAGAGTG AACCTGAAAA TCCTACCTTG 
CAGGAAACCA GTCTTACTGT CAAACTCTCT AACCTTGGAA TTGTGAGATC TCTGAGGACA 
AAGCAGCAGA CACAATCTCA GAATAAGTCT GTCTACATTG AATTGGGATC TGATTCTTCT 
GAAGACACAG TTAATAAGGC CAGTTATCTC AGTGTGGGAG ACCATGAATT GTTAGAAATC 
ACCCCTCAAG GAGCCAAGGC CAAGACCAAT TTGAATCCTG CAAAAAAGGC TGCTTGTGAG 
TTTTCTGAGA AGGACATAAC AAATACTGAA CATCATCAAC TCAATATTAA AGATCTGATC 
ACCACCCAGG AGCATGCAGC TGAGATGCAT GCAGCTGAGA CGCATGCAGC TGAGACGCAT 
CCAGAAAAGT ATCAGGGTAT TTCTGTTTCA GACTTTCACG TGGAGCCATG TGGCACAGAT 
ACTCATGCCA GCTCATTACA GCATGAGAAC AGCAGTTTAT TGCTCACTGA AAACAGACTG 
AATGTAGAAA AGGCTGAATT CTGTAATAAA AGCAAACAGC CTGTCTTAGT AAAGAGCCAA 
CAGAGCAGAT GGGCTGAAAG TAAGGGCACA TGTAAGGATA GGCAGATTCC CAGCACTGGG 
AAAAAGATAG TTCTGAATAC TGATCCCCTG TATGGAAGAA AAGAACTGTG TAAGCAGAAA 
CCTGCATGCT CTGACAGTCC TGGAGATTCC CAAGATGTTC CTTGGGTAAC CCTGAATAAT 
AGCATACAGA AAGTTAATGA CTGGTTTTCC AGAAGTGATG AAATATTAAC TTCTGACGAC 
TCGTGCGATG GGGGGTCTGA ATCAAATAAT GAAGTAGCTG GTGCAGTGGA AATTCCAAAT 
AACGTTGATG GATATTCAGG TTCTTCAGAG AAAATTGACT TAATGGCCAG TGATCCTCAT 
GGTACTTTAA TACATGAAAG AGTCCACTCC AAACCAGTAG AGAGTAATAT TGAAGATAAA 
ATATTTGGGA AAACCTATCG GAGGAAGTCG AGTCTCCCTA ACTTCAACCA CATAGCTGAA 
GATCTAATTA TAGGTGCATT TACTGTAGAA CCTCAGAAAA CACAAGAGCA GCCCCTCACA 
AATAAGCTAA AATGTAAAAG GAGAGGTACA TCAGGCCTTC AGCCTGAGGA TTTTATCAAG 
AAAGGTGATT TGACGATTGT TCCAAAGACT CCTGAAAAGA TGATTGAGAG AACTGACCAA 
ACAGAGCAGA AACATCACGG GATAAATATT ACTAGTGATG GTCATGAGAA TAAAACAAAA 
CGTGATGATG TTCAGAAAGA GCAAAACGCT AACCCAACAG AATCATTGGA AAACGAATCT 
GTTTTCAGAA CTAAGGCTGA ACCTATAAAC ACCAGTATAA GCAATATGGA ACTAGAATTG 
AATGTCCGCA GTTCAAAAGC ACCTAAGAAT AGGCTGAAGA GGAAATCCTC TACCAGGAAA 
ATTCCTGAAC TTGAACTAGT AGTCAGTAGA AATCCAAGTC CACCTAATCA TACTGAGCTA 
CTAATTGATA GCAGTTCTAG CAATGAAGAG ATGAAGAAAA AGCATTCTAG CCAAATGCCA 
GTCAGGCACA GCCAAAAGCT TCAACTCATG GGAGATAAAG AACCTACAGC TGGAGCCAAG 
AAGAGTAACA AGACATATGA ACAAATAAAT AAAAGACTTG CTAGTGATGC TTTTCCAGAA 
CTAAATTTTG CAAACACACC TGGTTATTTT ACTAACTGTT CTAGTAAACC TGAAGAGTTT 
GTTCATCCTA GCCTTCAAAG AGAGGAGAAT CTAGGAACAA TTCAAGTGTT GAATAATACC 
AAAGACCCCA AAGATCTGAT ATTAAGAGGA GGAAAAGCTT TGCAAATGGA AAGATCTGTA 
GAGAATACCA ATATTTCCTT GGTTCCTGAT ACTGATTATA GCACTCAGGA TAGTATCTCA 
TTACTAGAAG CTAAAACCCC AGAGAAGGCA AAGACTGCAC CAAATCCATG TGTGAGTCTG 
TGTGCAGCAA TCGAAAACCC CAAGGAACTT ATCCATAGGG ATTTTAAAGA TAACAAAAAC 
AACACAGAGG GCTTTCAGGG TCTCCTGGGA CATGACGTTA ACTATGTCAT TCAGGAGACA 
AGAGGAGAAA TGGAAGACAG TGAACTTGAT ACACAGTATT TGCAGAATAC GTTCAAGGTT 
TCAAAGCGTC AGACATTTGC TCTGTTTTCC AATCCAGGAA ATCCACAAAA GGAATGTGCC 
ACAGTCTTTG CCCACTCGGG GTCCTTAAAG GATCAAAGTC CAAGAGACCC CCTCAAATGC 
AGACAAAAAG AAGACAGTCG GGGAAAGAGT GAGTCTAAAA GCCAGCATGT GCAGGCCGTT 
TGTACAACAG TGCACTTTCC TGTGGCTGGT CAGAAAGATA AGACGCCGGG TGACGATGCC 
AAATGTAGTG CAAAAGAAGT CACTAGGGTT TGTCAGTCAT TACAATTGAG AGGCCACAAA 
ACTGAACTTG TTTTTGCTAA TAAGCAAGGG GTTTCAGAAA AACCAAATCT AACACCATCA 
CTTTTTCCCA TCAAGTCATC TGTTAAAACC GTATGTAAGA AAAGCCCATC AGAGAAATTT 
GAGGAACCTG TAATGTCACC TGAAAGAACA TTGGGAAGTG AGAGCATCAT TCAAAGTGCA 
GTGAGCACAA TCAGCCAAAA TGACATTCAA GAAAGCACTT TTAAAGAAGT CAACTCAAGC 
AGTGTTAATG AAGTAGGTTC CAGTACTAAT GAAGTAGGCT CTAGTATTAA TGAAGTAGGT 
TCCAGTGGTG AAAACATTCA AGCAGAACCA GGTAGAAACA GAGAACCTAA ATTAAGAGCT 
TTACTCAGAT TAGGTCTTAT GCAACCTGAA GTCTATAAGC AAAGCCTTCC TGTAAGTAAC 
TGTCACCATC CTGAAATAAA AAGGCAAGGA GAAAATGAGG ACATGCTTCA GGCTGTTAAG 
GCAGATTTCT CCCCATGTCT AATTTCAGAT AACCTCGAAC AACCTATGGG AAGCTGTCAT 
GCTTCTCAGG TTTGTTCTGA GACACCTGAC AACTTGTTAA ATGACGACGA AATAAAAGAA 
AATAGCCACT TTGCTGAAAG TGACATTAAG GAAAGATCTG CTGTTTTTAG TGAAAGTGTC 
CAAAAAGGAG AATTCAGAGG GAGCCCTGGC CCTTTCACCC ATACACATTT GGCTCAGGGT 
CACCAAAGAG GGGCTGGGAA ACTAGAGTCA GAAGAGACTG CGTCTAGTGA GGATGAAGAG 
CTTCCCTGCT TCCAACAATT ACTGTTTGGT AAAGTAACCG ATACACTGTC TCCATCTACT 
GGATGGAACA CTATTGCCAC TGAGGGTCTG TCTAAGGAAA CAGAGGAGAA CCTAGAATCA 
TTGAAGAGTG GCTTAAATGA CTGCAGTGGC CAGGGAACAT CAGCCAAGGT ATCCCAGGAA 
CATCACCTTA ATGAGGAAGT GAGATGTTCT GGTAGCTTAT TTTCCTCACA GTACAGTGCA 
GTGGAAGACT TGACCACTAA TACAAACACC CAGGATCCTT TCTTGATGTT TGAGCGTCCT 
TCCAAACAAG TGTATCAGTC TGAAAGCGAG GAAGTTCTGA GTGACAAGGA ATTTGTTTCA 
GATGATGAAG AAAGGGAAAC TGGCTTGGAA GAAGATAGTT GCCAAGAAGA GCAGAGTGTG 
GATTCAGACT TAGGTGAAGC AGCATCCGAC CATGTGAGTG AAACAAGCCT CTCTGAAGAC 
TGTGTGGGGC TGTCCTCTCA GAGTGATATC TTAACCACTC AGCAGAGGGA TACCATGCAA 
GATAACCTTC TAAAGCTCCA GCAGGAAATG GCCGAGCTGG AAGCTGTGTT AGAGCAGCAC 
GGGAGCCAGC CTTCCCACAG CTCTGCTTCC CTCACAGCTG ATTCTCGTGG CCCAGAGCAC 
CTGCTAAATC TAGAGCAAGA CACATCAGAA AGAGCAATAT TAACTTCAGA GAAAAGTAGA 
GACTGTTCTA GAAGCCAGAC TCCTGAAAGC CTGTCTGCTG ACAAGTTTCC AGTATCTCTG 
GATAGTTCTA CCAATAAAAA TAAAGAACCA GGAATGGAAA GATCTTCCCC TTCTAAATCC 
CAGTTGCCGG GTAATAGGTG GTATATGCAC AGCTCAAGGA GTCTTCAGGA CAGAAACTGC 
CCATCTCAAA AGAAGCGCAT TAATGTTGCT GACATGCAGG AGCAACAACT GGCTAAGTGT 
GAGGCCCAAG ATTTAATGGG GTCTTATTTG CCGAGACAAG ATCAAGAGGG AACCCCTTAC 
CTGAAATCTG GAATCAGCCT CTTCTCTCAT GAACCCGAGT CTGATCCCTC TGAAGACAGA 
GCCCCAGAGC CAGCTCATGT TCACAGCATG CCACCCACAG CCTCTGCACT GAAATTATCC 
CAATTTCGAG TAGAAGAATC TACCAAGAAT CCAGCTGCTG CTCACATTAC TAATACTACA 
GGGTGTAACC TGAGGGAAGA AAGTGTGAGC AAAGAGAAGC CAGAAGTGTT ATCTTCAACA 
GAAAGAAGCA ACAAAAGACT TTCTATGGTG GCATCAGGCT TGACCCCAAA AGAACTCATG 
CTTGTGCAGA AATTTGCCAG AAAACATCAC GTCACTTTAA CTAATCTAAT TACTGAAGAG 
ACTACTCATG TCATTATGAA AACAGATCCT GAGTTTGTGT GTGAACGGAC ACTGAAATAT 
TTTCTGGGAA TTGCAGGAGG AAAATGGGTA GTTAGCTATT TTTGGGTCAT CCAGTCTATT 
AAAGAAAGAA AGATGCTGGA TGAGCATGAT TTTGAAGTCA GAGGAGATGT TGTCAATGGA 
AGAAACCATC AAGGCCCAAA ACGAGCAAGA GAATCCCGGG ACAAGAAGAT CTTCAAGGGC 
CTGGAAATCT GTTGCTATGG ACCTTTTACC AATATGCCCA CAGATCAACT AGAATGGATG 
GTGCAGCTGT GTGGGGCTTC TGTGGTGAAG GAGCCCTCAT CATTCACCCC AGGCCAGGGT 
ACTCACCCAG TTGTGGTTGT GCAGCCGGAT GCCTGGACAG AGGATGCTGG CTTCCATGTG 
ATCGGGCAGA TGTGTGAAGC ACCTGTGGTA ACCCGAGAGT GGGTACTGGA CAGTGTGGCC 
CTTTACCAGT GCCAGGAGCT GGACACCTAC CTGGTCCCCC AGGTCGCCCA GAGCTGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012007781.1
CDS	210..5672
Translation	MLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLNIKDLITTQEHAAEMHAAETHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQESTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESEETASSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQTPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_012152391.2
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012152391.2

ORF Insert Sequence:

ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAAT GTCCATTGTG TAAGAATGAT 
ATAACCAAAA GAAGCCTACA AGAAAGTACA AGATTTAGTC AACTTGTTGA AGAGCTGTTG 
AAGATCATTC ATGCTTTTGA GCTTGACACA GGATTGCAGT TTGCAAACAG CTATAACTTT 
TCAAGAAAGG AAAATAACTC TCCTGAGCAT CTGAAGGAGG AAGTTTCTAT AATCCAAAGT 
ATGGGCTACC GGAACCGGGC CAAAAGACTT TGGCAGAGTG AACCTGAAAA TCCTACCTTG 
CAGGAAACCA GTCTTACTGT CAAACTCTCT AACCTTGGAA TTGTGAGATC TCTGAGGACA 
AAGCAGCAGA CACAATCTCA GAATAAGTCT GTCTACATTG AATTGGGATC TGATTCTTCT 
GAAGACACAG TTAATAAGGC CAGTTATCTC AGTGTGGGAG ACCATGAATT GTTAGAAATC 
ACCCCTCAAG GAGCCAAGGC CAAGACCAAT TTGAATCCTG CAAAAAAGGC TGCTTGTGAG 
TTTTCTGAGA AGGACATAAC AAATACTGAA CATCATCAAC TCAATATTAA AGATCTGATC 
ACCACCCAGG AGCATGCAGC TGAGATGCAT GCAGCTGAGA CGCATGCAGC TGAGACGCAT 
CCAGAAAAGT ATCAGGGTAT TTCTGTTTCA GACTTTCACG TGGAGCCATG TGGCACAGAT 
ACTCATGCCA GCTCATTACA GCATGAGAAC AGCAGTTTAT TGCTCACTGA AAACAGACTG 
AATGTAGAAA AGGCTGAATT CTGTAATAAA AGCAAACAGC CTGTCTTAGT AAAGAGCCAA 
CAGAGCAGAT GGGCTGAAAG TAAGGGCACA TGTAAGGATA GGCAGATTCC CAGCACTGGG 
AAAAAGATAG TTCTGAATAC TGATCCCCTG TATGGAAGAA AAGAACTGTG TAAGCAGAAA 
CCTGCATGCT CTGACAGTCC TGGAGATTCC CAAGATGTTC CTTGGGTAAC CCTGAATAAT 
AGCATACAGA AAGTTAATGA CTGGTTTTCC AGAAGTGATG AAATATTAAC TTCTGACGAC 
TCGTGCGATG GGGGGTCTGA ATCAAATAAT GAAGTAGCTG GTGCAGTGGA AATTCCAAAT 
AACGTTGATG GATATTCAGG TTCTTCAGAG AAAATTGACT TAATGGCCAG TGATCCTCAT 
GGTACTTTAA TACATGAAAG AGTCCACTCC AAACCAGTAG AGAGTAATAT TGAAGATAAA 
ATATTTGGGA AAACCTATCG GAGGAAGTCG AGTCTCCCTA ACTTCAACCA CATAGCTGAA 
GATCTAATTA TAGGTGCATT TACTGTAGAA CCTCAGAAAA CACAAGAGCA GCCCCTCACA 
AATAAGCTAA AATGTAAAAG GAGAGGTACA TCAGGCCTTC AGCCTGAGGA TTTTATCAAG 
AAAGGTGATT TGACGATTGT TCCAAAGACT CCTGAAAAGA TGATTGAGAG AACTGACCAA 
ACAGAGCAGA AACATCACGG GATAAATATT ACTAGTGATG GTCATGAGAA TAAAACAAAA 
CGTGATGATG TTCAGAAAGA GCAAAACGCT AACCCAACAG AATCATTGGA AAACGAATCT 
GTTTTCAGAA CTAAGGCTGA ACCTATAAAC ACCAGTATAA GCAATATGGA ACTAGAATTG 
AATGTCCGCA GTTCAAAAGC ACCTAAGAAT AGGCTGAAGA GGAAATCCTC TACCAGGAAA 
ATTCCTGAAC TTGAACTAGT AGTCAGTAGA AATCCAAGTC CACCTAATCA TACTGAGCTA 
CTAATTGATA GCAGTTCTAG CAATGAAGAG ATGAAGAAAA AGCATTCTAG CCAAATGCCA 
GTCAGGCACA GCCAAAAGCT TCAACTCATG GGAGATAAAG AACCTACAGC TGGAGCCAAG 
AAGAGTAACA AGACATATGA ACAAATAAAT AAAAGACTTG CTAGTGATGC TTTTCCAGAA 
CTAAATTTTG CAAACACACC TGGTTATTTT ACTAACTGTT CTAGTAAACC TGAAGAGTTT 
GTTCATCCTA GCCTTCAAAG AGAGGAGAAT CTAGGAACAA TTCAAGTGTT GAATAATACC 
AAAGACCCCA AAGATCTGAT ATTAAGAGGA GGAAAAGCTT TGCAAATGGA AAGATCTGTA 
GAGAATACCA ATATTTCCTT GGTTCCTGAT ACTGATTATA GCACTCAGGA TAGTATCTCA 
TTACTAGAAG CTAAAACCCC AGAGAAGGCA AAGACTGCAC CAAATCCATG TGTGAGTCTG 
TGTGCAGCAA TCGAAAACCC CAAGGAACTT ATCCATAGGG ATTTTAAAGA TAACAAAAAC 
AACACAGAGG GCTTTCAGGG TCTCCTGGGA CATGACGTTA ACTATGTCAT TCAGGAGACA 
AGAGGAGAAA TGGAAGACAG TGAACTTGAT ACACAGTATT TGCAGAATAC GTTCAAGGTT 
TCAAAGCGTC AGACATTTGC TCTGTTTTCC AATCCAGGAA ATCCACAAAA GGAATGTGCC 
ACAGTCTTTG CCCACTCGGG GTCCTTAAAG GATCAAAGTC CAAGAGACCC CCTCAAATGC 
AGACAAAAAG AAGACAGTCG GGGAAAGAGT GAGTCTAAAA GCCAGCATGT GCAGGCCGTT 
TGTACAACAG TGCACTTTCC TGTGGCTGGT CAGAAAGATA AGACGCCGGG TGACGATGCC 
AAATGTAGTG CAAAAGAAGT CACTAGGGTT TGTCAGTCAT TACAATTGAG AGGCCACAAA 
ACTGAACTTG TTTTTGCTAA TAAGCAAGGG GTTTCAGAAA AACCAAATCT AACACCATCA 
CTTTTTCCCA TCAAGTCATC TGTTAAAACC GTATGTAAGA AAAGCCCATC AGAGAAATTT 
GAGGAACCTG TAATGTCACC TGAAAGAACA TTGGGAAGTG AGAGCATCAT TCAAAGTGCA 
GTGAGCACAA TCAGCCAAAA TGACATTCAA GAAAGCACTT TTAAAGAAGT CAACTCAAGC 
AGTGTTAATG AAGTAGGTTC CAGTACTAAT GAAGTAGGCT CTAGTATTAA TGAAGTAGGT 
TCCAGTGGTG AAAACATTCA AGCAGAACCA GGTAGAAACA GAGAACCTAA ATTAAGAGCT 
TTACTCAGAT TAGGTCTTAT GCAACCTGAA GTCTATAAGC AAAGCCTTCC TGTAAGTAAC 
TGTCACCATC CTGAAATAAA AAGGCAAGGA GAAAATGAGG ACATGCTTCA GGCTGTTAAG 
GCAGATTTCT CCCCATGTCT AATTTCAGAT AACCTCGAAC AACCTATGGG AAGCTGTCAT 
GCTTCTCAGG TTTGTTCTGA GACACCTGAC AACTTGTTAA ATGACGACGA AATAAAAGAA 
AATAGCCACT TTGCTGAAAG TGACATTAAG GAAAGATCTG CTGTTTTTAG TGAAAGTGTC 
CAAAAAGGAG AATTCAGAGG GAGCCCTGGC CCTTTCACCC ATACACATTT GGCTCAGGGT 
CACCAAAGAG GGGCTGGGAA ACTAGAGTCA GAAGAGACTG CGTCTAGTGA GGATGAAGAG 
CTTCCCTGCT TCCAACAATT ACTGTTTGGT AAAGTAACCG ATACACTGTC TCCATCTACT 
GGATGGAACA CTATTGCCAC TGAGGGTCTG TCTAAGGAAA CAGAGGAGAA CCTAGAATCA 
TTGAAGAGTG GCTTAAATGA CTGCAGTGGC CAGGGAACAT CAGCCAAGGT ATCCCAGGAA 
CATCACCTTA ATGAGGAAGT GAGATGTTCT GGTAGCTTAT TTTCCTCACA GTACAGTGCA 
GTGGAAGACT TGACCACTAA TACAAACACC CAGGATCCTT TCTTGATGTT TGAGCGTCCT 
TCCAAACAAG TGTATCAGTC TGAAAGCGAG GAAGTTCTGA GTGACAAGGA ATTTGTTTCA 
GATGATGAAG AAAGGGAAAC TGGCTTGGAA GAAGATAGTT GCCAAGAAGA GCAGAGTGTG 
GATTCAGACT TAGGTGAAGC AGCATCCGAC CATGTGAGTG AAACAAGCCT CTCTGAAGAC 
TGTGTGGGGC TGTCCTCTCA GAGTGATATC TTAACCACTC AGCAGAGGGA TACCATGCAA 
GATAACCTTC TAAAGCTCCA GCAGGAAATG GCCGAGCTGG AAGCTGTGTT AGAGCAGCAC 
GGGAGCCAGC CTTCCCACAG CTCTGCTTCC CTCACAGCTG ATTCTCGTGG CCCAGAGCAC 
CTGCTAAATC TAGAGCAAGA CACATCAGAA AGAGCAATAT TAACTTCAGA GAAAAGTAGA 
GACTGTTCTA GAAGCCAGAC TCCTGAAAGC CTGTCTGCTG ACAAGTTTCC AGTATCTCTG 
GATAGTTCTA CCAATAAAAA TAAAGAACCA GGAATGGAAA GATCTTCCCC TTCTAAATCC 
CAGTTGCCGG GTAATAGGTG GTATATGCAC AGCTCAAGGA GTCTTCAGGA CAGAAACTGC 
CCATCTCAAA AGAAGCGCAT TAATGTTGCT GACATGCAGG AGCAACAACT GGCTAAGTGT 
GAGGCCCAAG ATTTAATGGG GTCTTATTTG CCGAGACAAG ATCAAGAGGG AACCCCTTAC 
CTGAAATCTG GAATCAGCCT CTTCTCTCAT GAACCCGAGT CTGATCCCTC TGAAGACAGA 
GCCCCAGAGC CAGCTCATGT TCACAGCATG CCACCCACAG CCTCTGCACT GAAATTATCC 
CAATTTCGAG TAGAAGAATC TACCAAGAAT CCAGCTGCTG CTCACATTAC TAATACTACA 
GGGTGTAACC TGAGGGAAGA AAGTGTGAGC AAAGAGAAGC CAGAAGTGTT ATCTTCAACA 
GAAAGAAGCA ACAAAAGACT TTCTATGGTG GCATCAGGCT TGACCCCAAA AGAACTCATG 
CTTGTGCAGA AATTTGCCAG AAAACATCAC GTCACTTTAA CTAATCTAAT TACTGAAGAG 
ACTACTCATG TCATTATGAA AACAGATCCT GAGTTTGTGT GTGAACGGAC ACTGAAATAT 
TTTCTGGGAA TTGCAGGAGG AAAATGGGTA GTTAGCTATT TTTGGGTCAT CCAGTCTATT 
AAAGAAAGAA AGATGCTGGA TGAGCATGAT TTTGAAGTCA GAGGAGATGT TGTCAATGGA 
AGAAACCATC AAGGCCCAAA ACGAGCAAGA GAATCCCGGG ACAAGAAGAT CTTCAAGGGC 
CTGGAAATCT GTTGCTATGG ACCTTTTACC AATATGCCCA CAGATCAACT AGAATGGATG 
GTGCAGCTGT GTGGGGCTTC TGTGGTGAAG GAGCCCTCAT CATTCACCCC AGGCCAGGGT 
ACTCACCCAG TTGTGGTTGT GCAGCCGGAT GCCTGGACAG AGGATGCTGG CTTCCATGTG 
ATCGGGCAGA TGTGTGAAGC ACCTGTGGTA ACCCGAGAGT GGGTACTGGA CAGTGTGGCC 
CTTTACCAGT GCCAGGAGCT GGACACCTAC CTGGTCCCCC AGGTCGCCCA GAGCTGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_012152392.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012007782.1 Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X6, mRNA.

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CloneID	OOv27530
Clone ID Related Accession (Same CDS sequence)	XM_012152391.2 , XM_012152392.1
Accession Version	XM_012152392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries musimon(Ovis orientalis musimon)
Product	breast cancer type 1 susceptibility protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011942684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAAT GTCCATTGTG TAAGAATGAT 
ATAACCAAAA GAAGCCTACA AGAAAGTACA AGATTTAGTC AACTTGTTGA AGAGCTGTTG 
AAGATCATTC ATGCTTTTGA GCTTGACACA GGATTGCAGT TTGCAAACAG CTATAACTTT 
TCAAGAAAGG AAAATAACTC TCCTGAGCAT CTGAAGGAGG AAGTTTCTAT AATCCAAAGT 
ATGGGCTACC GGAACCGGGC CAAAAGACTT TGGCAGAGTG AACCTGAAAA TCCTACCTTG 
CAGGAAACCA GTCTTACTGT CAAACTCTCT AACCTTGGAA TTGTGAGATC TCTGAGGACA 
AAGCAGCAGA CACAATCTCA GAATAAGTCT GTCTACATTG AATTGGGATC TGATTCTTCT 
GAAGACACAG TTAATAAGGC CAGTTATCTC AGTGTGGGAG ACCATGAATT GTTAGAAATC 
ACCCCTCAAG GAGCCAAGGC CAAGACCAAT TTGAATCCTG CAAAAAAGGC TGCTTGTGAG 
TTTTCTGAGA AGGACATAAC AAATACTGAA CATCATCAAC TCAATATTAA AGATCTGATC 
ACCACCCAGG AGCATGCAGC TGAGATGCAT GCAGCTGAGA CGCATGCAGC TGAGACGCAT 
CCAGAAAAGT ATCAGGGTAT TTCTGTTTCA GACTTTCACG TGGAGCCATG TGGCACAGAT 
ACTCATGCCA GCTCATTACA GCATGAGAAC AGCAGTTTAT TGCTCACTGA AAACAGACTG 
AATGTAGAAA AGGCTGAATT CTGTAATAAA AGCAAACAGC CTGTCTTAGT AAAGAGCCAA 
CAGAGCAGAT GGGCTGAAAG TAAGGGCACA TGTAAGGATA GGCAGATTCC CAGCACTGGG 
AAAAAGATAG TTCTGAATAC TGATCCCCTG TATGGAAGAA AAGAACTGTG TAAGCAGAAA 
CCTGCATGCT CTGACAGTCC TGGAGATTCC CAAGATGTTC CTTGGGTAAC CCTGAATAAT 
AGCATACAGA AAGTTAATGA CTGGTTTTCC AGAAGTGATG AAATATTAAC TTCTGACGAC 
TCGTGCGATG GGGGGTCTGA ATCAAATAAT GAAGTAGCTG GTGCAGTGGA AATTCCAAAT 
AACGTTGATG GATATTCAGG TTCTTCAGAG AAAATTGACT TAATGGCCAG TGATCCTCAT 
GGTACTTTAA TACATGAAAG AGTCCACTCC AAACCAGTAG AGAGTAATAT TGAAGATAAA 
ATATTTGGGA AAACCTATCG GAGGAAGTCG AGTCTCCCTA ACTTCAACCA CATAGCTGAA 
GATCTAATTA TAGGTGCATT TACTGTAGAA CCTCAGAAAA CACAAGAGCA GCCCCTCACA 
AATAAGCTAA AATGTAAAAG GAGAGGTACA TCAGGCCTTC AGCCTGAGGA TTTTATCAAG 
AAAGGTGATT TGACGATTGT TCCAAAGACT CCTGAAAAGA TGATTGAGAG AACTGACCAA 
ACAGAGCAGA AACATCACGG GATAAATATT ACTAGTGATG GTCATGAGAA TAAAACAAAA 
CGTGATGATG TTCAGAAAGA GCAAAACGCT AACCCAACAG AATCATTGGA AAACGAATCT 
GTTTTCAGAA CTAAGGCTGA ACCTATAAAC ACCAGTATAA GCAATATGGA ACTAGAATTG 
AATGTCCGCA GTTCAAAAGC ACCTAAGAAT AGGCTGAAGA GGAAATCCTC TACCAGGAAA 
ATTCCTGAAC TTGAACTAGT AGTCAGTAGA AATCCAAGTC CACCTAATCA TACTGAGCTA 
CTAATTGATA GCAGTTCTAG CAATGAAGAG ATGAAGAAAA AGCATTCTAG CCAAATGCCA 
GTCAGGCACA GCCAAAAGCT TCAACTCATG GGAGATAAAG AACCTACAGC TGGAGCCAAG 
AAGAGTAACA AGACATATGA ACAAATAAAT AAAAGACTTG CTAGTGATGC TTTTCCAGAA 
CTAAATTTTG CAAACACACC TGGTTATTTT ACTAACTGTT CTAGTAAACC TGAAGAGTTT 
GTTCATCCTA GCCTTCAAAG AGAGGAGAAT CTAGGAACAA TTCAAGTGTT GAATAATACC 
AAAGACCCCA AAGATCTGAT ATTAAGAGGA GGAAAAGCTT TGCAAATGGA AAGATCTGTA 
GAGAATACCA ATATTTCCTT GGTTCCTGAT ACTGATTATA GCACTCAGGA TAGTATCTCA 
TTACTAGAAG CTAAAACCCC AGAGAAGGCA AAGACTGCAC CAAATCCATG TGTGAGTCTG 
TGTGCAGCAA TCGAAAACCC CAAGGAACTT ATCCATAGGG ATTTTAAAGA TAACAAAAAC 
AACACAGAGG GCTTTCAGGG TCTCCTGGGA CATGACGTTA ACTATGTCAT TCAGGAGACA 
AGAGGAGAAA TGGAAGACAG TGAACTTGAT ACACAGTATT TGCAGAATAC GTTCAAGGTT 
TCAAAGCGTC AGACATTTGC TCTGTTTTCC AATCCAGGAA ATCCACAAAA GGAATGTGCC 
ACAGTCTTTG CCCACTCGGG GTCCTTAAAG GATCAAAGTC CAAGAGACCC CCTCAAATGC 
AGACAAAAAG AAGACAGTCG GGGAAAGAGT GAGTCTAAAA GCCAGCATGT GCAGGCCGTT 
TGTACAACAG TGCACTTTCC TGTGGCTGGT CAGAAAGATA AGACGCCGGG TGACGATGCC 
AAATGTAGTG CAAAAGAAGT CACTAGGGTT TGTCAGTCAT TACAATTGAG AGGCCACAAA 
ACTGAACTTG TTTTTGCTAA TAAGCAAGGG GTTTCAGAAA AACCAAATCT AACACCATCA 
CTTTTTCCCA TCAAGTCATC TGTTAAAACC GTATGTAAGA AAAGCCCATC AGAGAAATTT 
GAGGAACCTG TAATGTCACC TGAAAGAACA TTGGGAAGTG AGAGCATCAT TCAAAGTGCA 
GTGAGCACAA TCAGCCAAAA TGACATTCAA GAAAGCACTT TTAAAGAAGT CAACTCAAGC 
AGTGTTAATG AAGTAGGTTC CAGTACTAAT GAAGTAGGCT CTAGTATTAA TGAAGTAGGT 
TCCAGTGGTG AAAACATTCA AGCAGAACCA GGTAGAAACA GAGAACCTAA ATTAAGAGCT 
TTACTCAGAT TAGGTCTTAT GCAACCTGAA GTCTATAAGC AAAGCCTTCC TGTAAGTAAC 
TGTCACCATC CTGAAATAAA AAGGCAAGGA GAAAATGAGG ACATGCTTCA GGCTGTTAAG 
GCAGATTTCT CCCCATGTCT AATTTCAGAT AACCTCGAAC AACCTATGGG AAGCTGTCAT 
GCTTCTCAGG TTTGTTCTGA GACACCTGAC AACTTGTTAA ATGACGACGA AATAAAAGAA 
AATAGCCACT TTGCTGAAAG TGACATTAAG GAAAGATCTG CTGTTTTTAG TGAAAGTGTC 
CAAAAAGGAG AATTCAGAGG GAGCCCTGGC CCTTTCACCC ATACACATTT GGCTCAGGGT 
CACCAAAGAG GGGCTGGGAA ACTAGAGTCA GAAGAGACTG CGTCTAGTGA GGATGAAGAG 
CTTCCCTGCT TCCAACAATT ACTGTTTGGT AAAGTAACCG ATACACTGTC TCCATCTACT 
GGATGGAACA CTATTGCCAC TGAGGGTCTG TCTAAGGAAA CAGAGGAGAA CCTAGAATCA 
TTGAAGAGTG GCTTAAATGA CTGCAGTGGC CAGGGAACAT CAGCCAAGGT ATCCCAGGAA 
CATCACCTTA ATGAGGAAGT GAGATGTTCT GGTAGCTTAT TTTCCTCACA GTACAGTGCA 
GTGGAAGACT TGACCACTAA TACAAACACC CAGGATCCTT TCTTGATGTT TGAGCGTCCT 
TCCAAACAAG TGTATCAGTC TGAAAGCGAG GAAGTTCTGA GTGACAAGGA ATTTGTTTCA 
GATGATGAAG AAAGGGAAAC TGGCTTGGAA GAAGATAGTT GCCAAGAAGA GCAGAGTGTG 
GATTCAGACT TAGGTGAAGC AGCATCCGAC CATGTGAGTG AAACAAGCCT CTCTGAAGAC 
TGTGTGGGGC TGTCCTCTCA GAGTGATATC TTAACCACTC AGCAGAGGGA TACCATGCAA 
GATAACCTTC TAAAGCTCCA GCAGGAAATG GCCGAGCTGG AAGCTGTGTT AGAGCAGCAC 
GGGAGCCAGC CTTCCCACAG CTCTGCTTCC CTCACAGCTG ATTCTCGTGG CCCAGAGCAC 
CTGCTAAATC TAGAGCAAGA CACATCAGAA AGAGCAATAT TAACTTCAGA GAAAAGTAGA 
GACTGTTCTA GAAGCCAGAC TCCTGAAAGC CTGTCTGCTG ACAAGTTTCC AGTATCTCTG 
GATAGTTCTA CCAATAAAAA TAAAGAACCA GGAATGGAAA GATCTTCCCC TTCTAAATCC 
CAGTTGCCGG GTAATAGGTG GTATATGCAC AGCTCAAGGA GTCTTCAGGA CAGAAACTGC 
CCATCTCAAA AGAAGCGCAT TAATGTTGCT GACATGCAGG AGCAACAACT GGCTAAGTGT 
GAGGCCCAAG ATTTAATGGG GTCTTATTTG CCGAGACAAG ATCAAGAGGG AACCCCTTAC 
CTGAAATCTG GAATCAGCCT CTTCTCTCAT GAACCCGAGT CTGATCCCTC TGAAGACAGA 
GCCCCAGAGC CAGCTCATGT TCACAGCATG CCACCCACAG CCTCTGCACT GAAATTATCC 
CAATTTCGAG TAGAAGAATC TACCAAGAAT CCAGCTGCTG CTCACATTAC TAATACTACA 
GGGTGTAACC TGAGGGAAGA AAGTGTGAGC AAAGAGAAGC CAGAAGTGTT ATCTTCAACA 
GAAAGAAGCA ACAAAAGACT TTCTATGGTG GCATCAGGCT TGACCCCAAA AGAACTCATG 
CTTGTGCAGA AATTTGCCAG AAAACATCAC GTCACTTTAA CTAATCTAAT TACTGAAGAG 
ACTACTCATG TCATTATGAA AACAGATCCT GAGTTTGTGT GTGAACGGAC ACTGAAATAT 
TTTCTGGGAA TTGCAGGAGG AAAATGGGTA GTTAGCTATT TTTGGGTCAT CCAGTCTATT 
AAAGAAAGAA AGATGCTGGA TGAGCATGAT TTTGAAGTCA GAGGAGATGT TGTCAATGGA 
AGAAACCATC AAGGCCCAAA ACGAGCAAGA GAATCCCGGG ACAAGAAGAT CTTCAAGGGC 
CTGGAAATCT GTTGCTATGG ACCTTTTACC AATATGCCCA CAGATCAACT AGAATGGATG 
GTGCAGCTGT GTGGGGCTTC TGTGGTGAAG GAGCCCTCAT CATTCACCCC AGGCCAGGGT 
ACTCACCCAG TTGTGGTTGT GCAGCCGGAT GCCTGGACAG AGGATGCTGG CTTCCATGTG 
ATCGGGCAGA TGTGTGAAGC ACCTGTGGTA ACCCGAGAGT GGGTACTGGA CAGTGTGGCC 
CTTTACCAGT GCCAGGAGCT GGACACCTAC CTGGTCCCCC AGGTCGCCCA GAGCTGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012007782.1
CDS	119..5581
Translation	MLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLNIKDLITTQEHAAEMHAAETHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQESTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESEETASSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQTPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_012152392.1
Organism	Ovis aries musimon(Ovis orientalis musimon)
Definition	Ovis aries musimon breast cancer 1 (BRCA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012152392.1

ORF Insert Sequence:

ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAAT GTCCATTGTG TAAGAATGAT 
ATAACCAAAA GAAGCCTACA AGAAAGTACA AGATTTAGTC AACTTGTTGA AGAGCTGTTG 
AAGATCATTC ATGCTTTTGA GCTTGACACA GGATTGCAGT TTGCAAACAG CTATAACTTT 
TCAAGAAAGG AAAATAACTC TCCTGAGCAT CTGAAGGAGG AAGTTTCTAT AATCCAAAGT 
ATGGGCTACC GGAACCGGGC CAAAAGACTT TGGCAGAGTG AACCTGAAAA TCCTACCTTG 
CAGGAAACCA GTCTTACTGT CAAACTCTCT AACCTTGGAA TTGTGAGATC TCTGAGGACA 
AAGCAGCAGA CACAATCTCA GAATAAGTCT GTCTACATTG AATTGGGATC TGATTCTTCT 
GAAGACACAG TTAATAAGGC CAGTTATCTC AGTGTGGGAG ACCATGAATT GTTAGAAATC 
ACCCCTCAAG GAGCCAAGGC CAAGACCAAT TTGAATCCTG CAAAAAAGGC TGCTTGTGAG 
TTTTCTGAGA AGGACATAAC AAATACTGAA CATCATCAAC TCAATATTAA AGATCTGATC 
ACCACCCAGG AGCATGCAGC TGAGATGCAT GCAGCTGAGA CGCATGCAGC TGAGACGCAT 
CCAGAAAAGT ATCAGGGTAT TTCTGTTTCA GACTTTCACG TGGAGCCATG TGGCACAGAT 
ACTCATGCCA GCTCATTACA GCATGAGAAC AGCAGTTTAT TGCTCACTGA AAACAGACTG 
AATGTAGAAA AGGCTGAATT CTGTAATAAA AGCAAACAGC CTGTCTTAGT AAAGAGCCAA 
CAGAGCAGAT GGGCTGAAAG TAAGGGCACA TGTAAGGATA GGCAGATTCC CAGCACTGGG 
AAAAAGATAG TTCTGAATAC TGATCCCCTG TATGGAAGAA AAGAACTGTG TAAGCAGAAA 
CCTGCATGCT CTGACAGTCC TGGAGATTCC CAAGATGTTC CTTGGGTAAC CCTGAATAAT 
AGCATACAGA AAGTTAATGA CTGGTTTTCC AGAAGTGATG AAATATTAAC TTCTGACGAC 
TCGTGCGATG GGGGGTCTGA ATCAAATAAT GAAGTAGCTG GTGCAGTGGA AATTCCAAAT 
AACGTTGATG GATATTCAGG TTCTTCAGAG AAAATTGACT TAATGGCCAG TGATCCTCAT 
GGTACTTTAA TACATGAAAG AGTCCACTCC AAACCAGTAG AGAGTAATAT TGAAGATAAA 
ATATTTGGGA AAACCTATCG GAGGAAGTCG AGTCTCCCTA ACTTCAACCA CATAGCTGAA 
GATCTAATTA TAGGTGCATT TACTGTAGAA CCTCAGAAAA CACAAGAGCA GCCCCTCACA 
AATAAGCTAA AATGTAAAAG GAGAGGTACA TCAGGCCTTC AGCCTGAGGA TTTTATCAAG 
AAAGGTGATT TGACGATTGT TCCAAAGACT CCTGAAAAGA TGATTGAGAG AACTGACCAA 
ACAGAGCAGA AACATCACGG GATAAATATT ACTAGTGATG GTCATGAGAA TAAAACAAAA 
CGTGATGATG TTCAGAAAGA GCAAAACGCT AACCCAACAG AATCATTGGA AAACGAATCT 
GTTTTCAGAA CTAAGGCTGA ACCTATAAAC ACCAGTATAA GCAATATGGA ACTAGAATTG 
AATGTCCGCA GTTCAAAAGC ACCTAAGAAT AGGCTGAAGA GGAAATCCTC TACCAGGAAA 
ATTCCTGAAC TTGAACTAGT AGTCAGTAGA AATCCAAGTC CACCTAATCA TACTGAGCTA 
CTAATTGATA GCAGTTCTAG CAATGAAGAG ATGAAGAAAA AGCATTCTAG CCAAATGCCA 
GTCAGGCACA GCCAAAAGCT TCAACTCATG GGAGATAAAG AACCTACAGC TGGAGCCAAG 
AAGAGTAACA AGACATATGA ACAAATAAAT AAAAGACTTG CTAGTGATGC TTTTCCAGAA 
CTAAATTTTG CAAACACACC TGGTTATTTT ACTAACTGTT CTAGTAAACC TGAAGAGTTT 
GTTCATCCTA GCCTTCAAAG AGAGGAGAAT CTAGGAACAA TTCAAGTGTT GAATAATACC 
AAAGACCCCA AAGATCTGAT ATTAAGAGGA GGAAAAGCTT TGCAAATGGA AAGATCTGTA 
GAGAATACCA ATATTTCCTT GGTTCCTGAT ACTGATTATA GCACTCAGGA TAGTATCTCA 
TTACTAGAAG CTAAAACCCC AGAGAAGGCA AAGACTGCAC CAAATCCATG TGTGAGTCTG 
TGTGCAGCAA TCGAAAACCC CAAGGAACTT ATCCATAGGG ATTTTAAAGA TAACAAAAAC 
AACACAGAGG GCTTTCAGGG TCTCCTGGGA CATGACGTTA ACTATGTCAT TCAGGAGACA 
AGAGGAGAAA TGGAAGACAG TGAACTTGAT ACACAGTATT TGCAGAATAC GTTCAAGGTT 
TCAAAGCGTC AGACATTTGC TCTGTTTTCC AATCCAGGAA ATCCACAAAA GGAATGTGCC 
ACAGTCTTTG CCCACTCGGG GTCCTTAAAG GATCAAAGTC CAAGAGACCC CCTCAAATGC 
AGACAAAAAG AAGACAGTCG GGGAAAGAGT GAGTCTAAAA GCCAGCATGT GCAGGCCGTT 
TGTACAACAG TGCACTTTCC TGTGGCTGGT CAGAAAGATA AGACGCCGGG TGACGATGCC 
AAATGTAGTG CAAAAGAAGT CACTAGGGTT TGTCAGTCAT TACAATTGAG AGGCCACAAA 
ACTGAACTTG TTTTTGCTAA TAAGCAAGGG GTTTCAGAAA AACCAAATCT AACACCATCA 
CTTTTTCCCA TCAAGTCATC TGTTAAAACC GTATGTAAGA AAAGCCCATC AGAGAAATTT 
GAGGAACCTG TAATGTCACC TGAAAGAACA TTGGGAAGTG AGAGCATCAT TCAAAGTGCA 
GTGAGCACAA TCAGCCAAAA TGACATTCAA GAAAGCACTT TTAAAGAAGT CAACTCAAGC 
AGTGTTAATG AAGTAGGTTC CAGTACTAAT GAAGTAGGCT CTAGTATTAA TGAAGTAGGT 
TCCAGTGGTG AAAACATTCA AGCAGAACCA GGTAGAAACA GAGAACCTAA ATTAAGAGCT 
TTACTCAGAT TAGGTCTTAT GCAACCTGAA GTCTATAAGC AAAGCCTTCC TGTAAGTAAC 
TGTCACCATC CTGAAATAAA AAGGCAAGGA GAAAATGAGG ACATGCTTCA GGCTGTTAAG 
GCAGATTTCT CCCCATGTCT AATTTCAGAT AACCTCGAAC AACCTATGGG AAGCTGTCAT 
GCTTCTCAGG TTTGTTCTGA GACACCTGAC AACTTGTTAA ATGACGACGA AATAAAAGAA 
AATAGCCACT TTGCTGAAAG TGACATTAAG GAAAGATCTG CTGTTTTTAG TGAAAGTGTC 
CAAAAAGGAG AATTCAGAGG GAGCCCTGGC CCTTTCACCC ATACACATTT GGCTCAGGGT 
CACCAAAGAG GGGCTGGGAA ACTAGAGTCA GAAGAGACTG CGTCTAGTGA GGATGAAGAG 
CTTCCCTGCT TCCAACAATT ACTGTTTGGT AAAGTAACCG ATACACTGTC TCCATCTACT 
GGATGGAACA CTATTGCCAC TGAGGGTCTG TCTAAGGAAA CAGAGGAGAA CCTAGAATCA 
TTGAAGAGTG GCTTAAATGA CTGCAGTGGC CAGGGAACAT CAGCCAAGGT ATCCCAGGAA 
CATCACCTTA ATGAGGAAGT GAGATGTTCT GGTAGCTTAT TTTCCTCACA GTACAGTGCA 
GTGGAAGACT TGACCACTAA TACAAACACC CAGGATCCTT TCTTGATGTT TGAGCGTCCT 
TCCAAACAAG TGTATCAGTC TGAAAGCGAG GAAGTTCTGA GTGACAAGGA ATTTGTTTCA 
GATGATGAAG AAAGGGAAAC TGGCTTGGAA GAAGATAGTT GCCAAGAAGA GCAGAGTGTG 
GATTCAGACT TAGGTGAAGC AGCATCCGAC CATGTGAGTG AAACAAGCCT CTCTGAAGAC 
TGTGTGGGGC TGTCCTCTCA GAGTGATATC TTAACCACTC AGCAGAGGGA TACCATGCAA 
GATAACCTTC TAAAGCTCCA GCAGGAAATG GCCGAGCTGG AAGCTGTGTT AGAGCAGCAC 
GGGAGCCAGC CTTCCCACAG CTCTGCTTCC CTCACAGCTG ATTCTCGTGG CCCAGAGCAC 
CTGCTAAATC TAGAGCAAGA CACATCAGAA AGAGCAATAT TAACTTCAGA GAAAAGTAGA 
GACTGTTCTA GAAGCCAGAC TCCTGAAAGC CTGTCTGCTG ACAAGTTTCC AGTATCTCTG 
GATAGTTCTA CCAATAAAAA TAAAGAACCA GGAATGGAAA GATCTTCCCC TTCTAAATCC 
CAGTTGCCGG GTAATAGGTG GTATATGCAC AGCTCAAGGA GTCTTCAGGA CAGAAACTGC 
CCATCTCAAA AGAAGCGCAT TAATGTTGCT GACATGCAGG AGCAACAACT GGCTAAGTGT 
GAGGCCCAAG ATTTAATGGG GTCTTATTTG CCGAGACAAG ATCAAGAGGG AACCCCTTAC 
CTGAAATCTG GAATCAGCCT CTTCTCTCAT GAACCCGAGT CTGATCCCTC TGAAGACAGA 
GCCCCAGAGC CAGCTCATGT TCACAGCATG CCACCCACAG CCTCTGCACT GAAATTATCC 
CAATTTCGAG TAGAAGAATC TACCAAGAAT CCAGCTGCTG CTCACATTAC TAATACTACA 
GGGTGTAACC TGAGGGAAGA AAGTGTGAGC AAAGAGAAGC CAGAAGTGTT ATCTTCAACA 
GAAAGAAGCA ACAAAAGACT TTCTATGGTG GCATCAGGCT TGACCCCAAA AGAACTCATG 
CTTGTGCAGA AATTTGCCAG AAAACATCAC GTCACTTTAA CTAATCTAAT TACTGAAGAG 
ACTACTCATG TCATTATGAA AACAGATCCT GAGTTTGTGT GTGAACGGAC ACTGAAATAT 
TTTCTGGGAA TTGCAGGAGG AAAATGGGTA GTTAGCTATT TTTGGGTCAT CCAGTCTATT 
AAAGAAAGAA AGATGCTGGA TGAGCATGAT TTTGAAGTCA GAGGAGATGT TGTCAATGGA 
AGAAACCATC AAGGCCCAAA ACGAGCAAGA GAATCCCGGG ACAAGAAGAT CTTCAAGGGC 
CTGGAAATCT GTTGCTATGG ACCTTTTACC AATATGCCCA CAGATCAACT AGAATGGATG 
GTGCAGCTGT GTGGGGCTTC TGTGGTGAAG GAGCCCTCAT CATTCACCCC AGGCCAGGGT 
ACTCACCCAG TTGTGGTTGT GCAGCCGGAT GCCTGGACAG AGGATGCTGG CTTCCATGTG 
ATCGGGCAGA TGTGTGAAGC ACCTGTGGTA ACCCGAGAGT GGGTACTGGA CAGTGTGGCC 
CTTTACCAGT GCCAGGAGCT GGACACCTAC CTGGTCCCCC AGGTCGCCCA GAGCTGCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015098800.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014954286.1 Ovis aries breast cancer 1 (BRCA1), transcript variant X1, mRNA.

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CloneID	OSm10053
Clone ID Related Accession (Same CDS sequence)	XM_015098800.1 , XM_015098805.1 , XM_015098801.1 , XM_015098802.1 , XM_015098804.1
Accession Version	XM_015098800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014954286.1
CDS	213..5801
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESAETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015098800.1
Organism	Ovis aries(sheep)
Definition	Ovis aries breast cancer 1 (BRCA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015098800.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015098805.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014954291.1 Ovis aries breast cancer 1 (BRCA1), transcript variant X8, mRNA.

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CloneID	OSm10053
Clone ID Related Accession (Same CDS sequence)	XM_015098800.1 , XM_015098805.1 , XM_015098801.1 , XM_015098802.1 , XM_015098804.1
Accession Version	XM_015098805.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014954291.1
CDS	188..5776
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESAETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015098805.1
Organism	Ovis aries(sheep)
Definition	Ovis aries breast cancer 1 (BRCA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015098805.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015098801.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014954287.1 Ovis aries breast cancer 1 (BRCA1), transcript variant X2, mRNA.

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CloneID	OSm10053
Clone ID Related Accession (Same CDS sequence)	XM_015098800.1 , XM_015098805.1 , XM_015098801.1 , XM_015098802.1 , XM_015098804.1
Accession Version	XM_015098801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014954287.1
CDS	206..5794
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESAETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015098801.1
Organism	Ovis aries(sheep)
Definition	Ovis aries breast cancer 1 (BRCA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015098801.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015098802.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014954288.1 Ovis aries breast cancer 1 (BRCA1), transcript variant X7, mRNA.

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CloneID	OSm10053
Clone ID Related Accession (Same CDS sequence)	XM_015098800.1 , XM_015098805.1 , XM_015098801.1 , XM_015098802.1 , XM_015098804.1
Accession Version	XM_015098802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014954288.1
CDS	201..5789
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESAETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015098802.1
Organism	Ovis aries(sheep)
Definition	Ovis aries breast cancer 1 (BRCA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015098802.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015098804.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014954290.1 Ovis aries breast cancer 1 (BRCA1), transcript variant X3, mRNA.

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CloneID	OSm10053
Clone ID Related Accession (Same CDS sequence)	XM_015098800.1 , XM_015098805.1 , XM_015098801.1 , XM_015098802.1 , XM_015098804.1
Accession Version	XM_015098804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014954290.1
CDS	194..5782
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESAETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015098804.1
Organism	Ovis aries(sheep)
Definition	Ovis aries breast cancer 1 (BRCA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015098804.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GCAGGAAACC AGTCTTACTG TCAAACTCTC TAACCTTGGA 
ATTGTGAGAT CTCTGAGGAC AAAGCAGCAG ACACAATCTC AGAATAAGTC TGTCTACATT 
GAATTGGGAT CTGATTCTTC TGAAGACACA GTTAATAAGG CCAGTTATCT CAGTGTGGGA 
GACCATGAAT TGTTAGAAAT CACCCCTCAA GGAGCCAAGG CCAAGACCAA TTTGAATCCT 
GCAAAAAAGG CTGCTTGTGA GTTTTCTGAG AAGGACATAA CAAATACTGA ACATCATCAA 
CTCAGTATTA AAGATCTGAT CACCACCCAG GAGCATGCAG CTGAGATGCA TGCAGCTGAG 
ACGCATCCAG AAAAGTATCA GGGTATTTCT GTTTCAGACT TTCACGTGGA GCCATGTGGC 
ACAGATACTC ATGCCAGCTC ATTACAGCAT GAGAACAGCA GTTTATTGCT CACTGAAAAC 
AGACTGAATG TAGAAAAGGC TGAATTCTGT AATAAAAGCA AACAGCCTGT CTTAGTAAAG 
AGCCAACAGA GCAGATGGGC TGAAAGTAAG GGCACATGTA AGGATAGGCA GATTCCCAGC 
ACTGGGAAAA AGATAGTTCT GAATACTGAT CCCCTGTATG GAAGAAAAGA ACTGTGTAAG 
CAGAAACCTG CATGCTCTGA CAGTCCTGGA GATTCCCAAG ATGTTCCTTG GGTAACCCTG 
AATAATAGCA TACAGAAAGT TAATGACTGG TTTTCCAGAA GTGATGAAAT ATTAACTTCT 
GACGACTCGT GCGATGGGGG GTCTGAATCA AATAATGAAG TAGCTGGTGC AGTGGAAATT 
CCAAATAACG TTGATGGATA TTCAGGTTCT TCAGAGAAAA TCGACTTAAT GGCCAGTGAT 
CCTCATGGTA CTTTAATACA TGAAAGAGTC CACTCCAAAC CAGTAGAGAG TAATATTGAA 
GATAAAATAT TTGGGAAAAC CTATCGGAGG AAGTCGAGTC TCCCTAACTT CAACCACATA 
GCTGAAGATC TAATTATAGG TGCATTTACT GTAGAACCTC AGAAAACACA AGAGCAGCCC 
CTCACAAATA AGCTAAAATG TAAAAGGAGA GGTACATCAG GCCTTCAGCC TGAGGATTTT 
ATCAAGAAAG GTGATTTGAC GATTGTTCCA AAGACTCCTG AAAAGATGAT TGAGAGAACT 
GACCAAACAG AGCAGAAACA TCACGGGATA AATATTACTA GTGATGGTCA TGAGAATAAA 
ACAAAACGTG ATGATGTTCA GAAAGAGCAA AACGCTAACC CAACAGAATC ATTGGAAAAC 
GAATCTGTTT TCAGAACTAA GGCTGAACCT ATAAACACCA GTATAAGCAA TATGGAACTA 
GAATTGAATG TCCGCAGTTC AAAAGCACCT AAGAATAGGC TGAAGAGGAA ATCCTCTACC 
AGGAAAATTC CTGAACTTGA ACTAGTAGTC AGTAGAAATC CAAGTCCACC TAATCATACT 
GAGCTACTAA TTGATAGCAG TTCTAGCAAT GAAGAGATGA AGAAAAAGCA TTCTAGCCAA 
ATGCCAGTCA GGCACAGCCA AAAGCTTCAA CTCATGGGAG ATAAAGAACC TACAGCTGGA 
GCCAAGAAGA GTAACAAAAC ATATGAACAA ATAAATAAAA GACTTGCTAG TGATGCTTTT 
CCAGAACTAA ATTTTGCAAA CACACCTGGT TATTTTACTA ACTGTTCTAG TAAACCTGAA 
GAGTTTGTTC ATCCTAGCCT TCAAAGAGAG GAGAATCTAG GAACAATTCA AGTGTTGAAT 
AATACCAAAG ACCCCAAAGA TCTGATATTA AGAGGAGGAA AAGCTTTGCA AATGGAAAGA 
TCTGTAGAGA ATACCAATAT TTCCTTGGTT CCTGATACTG ATTATAGCAC TCAGGATAGT 
ATCTCATTAC TAGAAGCTAA AACCCCAGAG AAGGCAAAGA CTGCACCAAA TCCATGTGTG 
AGTCTGTGTG CAGCAATCGA AAACCCCAAG GAACTTATCC ATAGGGATTT TAAAGATAAC 
AAAAACAACA CAGAGGGCTT TCAGGGTCTC CTGGGACATG ACGTTAACTA TGTCATTCAG 
GAGACAAGAG GAGAAATGGA AGACAGTGAA CTTGATACAC AGTATTTGCA GAATACGTTC 
AAGGTTTCAA AGCGTCAGAC ATTTGCTCTG TTTTCCAATC CAGGAAATCC ACAAAAGGAA 
TGTGCCACAG TCTTTGCCCA CTCGGGGTCC TTAAAGGATC AAAGTCCAAG AGACCCCCTC 
AAATGCAGAC AAAAAGAAGA CAGTCGGGGA AAGAGTGAGT CTAAAAGCCA GCATGTGCAG 
GCCGTTTGTA CAACAGTGCA CTTTCCTGTG GCTGGTCAGA AAGATAAGAC GCCGGGTGAC 
GATGCCAAAT GTAGTGCAAA AGAAGTCACT AGGGTTTGTC AGTCGTTACA ATTGAGAGGC 
CACAAAACTG AACTTGTTTT TGCTAATAAG CAAGGGGTTT CAGAAAAACC AAATCTAACA 
CCATCACTTT TTCCCATCAA GTCATCTGTT AAAACCGTAT GTAAGAAAAG CCCATCAGAG 
AAATTTGAGG AACCTGTAAT GTCACCTGAA AGAACATTGG GAAGTGAGAG CATCATTCAA 
AGTGCAGTGA GCACAATCAG CCAAAATGAC ATTCAAGGAA GCACTTTTAA AGAAGTCAAC 
TCAAGCAGTG TTAATGAAGT AGGTTCCAGT ACTAATGAAG TAGGCTCTAG TATTAATGAA 
GTAGGTTCCA GTGGTGAAAA CATTCAAGCA GAACCAGGTA GAAACAGAGA ACCTAAATTA 
AGAGCTTTAC TCAGATTAGG TCTTATGCAA CCTGAAGTCT ATAAGCAAAG CCTTCCTGTA 
AGTAACTGTC ACCATCCTGA AATAAAAAGG CAAGGAGAAA ATGAGGACAT GCTTCAGGCT 
GTTAAGGCAG ATTTCTCCCC ATGTCTAATT TCAGATAACC TCGAACAACC TATGGGAAGC 
TGTCATGCTT CTCAGGTTTG TTCTGAGACA CCTGACAACT TGTTAAATGA CGACGAAATA 
AAAGAAAATA GCCACTTTGC TGAAAGTGAC ATTAAGGAAA GATCTGCTGT TTTTAGTGAA 
AGTGTCCAAA AAGGAGAATT CAGAGGGAGC CCTGGCCCTT TCACCCATAC ACATTTGGCT 
CAGGGTCACC AAAGAGGGGC TGGGAAACTA GAGTCAGCAG AGACTGTGTC TAGTGAGGAT 
GAAGAGCTTC CCTGCTTCCA ACAATTACTG TTTGGTAAAG TAACCGATAC ACTGTCTCCA 
TCTACTGGAT GGAACACTAT TGCCACTGAG GGTCTGTCTA AGGAAACAGA GGAGAACCTA 
GAATCATTGA AGAGTGGCTT AAATGACTGC AGTGGCCAGG GAACATCAGC CAAGGTATCC 
CAGGAACATC ACCTTAATGA GGAAGTGAGA TGTTCTGGTA GCTTATTTTC CTCACAGTAC 
AGTGCAGTGG AAGACTTGAC CACTAATACA AACACCCAGG ATCCTTTCTT GATGTTTGAG 
CGTCCTTCCA AACAAGTGTA TCAGTCTGAA AGCGAGGAAG TTCTGAGTGA CAAGGAATTT 
GTTTCAGATG ATGAAGAAAG GGAAACTGGC TTGGAAGAAG ATAGTTGCCA AGAAGAGCAG 
AGTGTGGATT CAGACTTAGG TGAAGCAGCA TCCGACCATG TGAGTGAAAC AAGCCTCTCT 
GAAGACTGTG TGGGGCTGTC CTCTCAGAGT GATATCTTAA CCACTCAGCA GAGGGATACC 
ATGCAAGATA ACCTTCTAAA GCTCCAGCAG GAAATGGCCG AGCTGGAAGC TGTGTTAGAG 
CAGCACGGGA GCCAGCCTTC CCACAGCTCT GCTTCCCTCA CAGCTGATTC TCGTGGCCCA 
GAGCACCTGC TAAATCTAGA GCAAGACACA TCAGAAAGAG CAATATTAAC TTCAGAGAAA 
AGTAGAGACT GTTCTAGAAG CCAGAATCCT GAAAGCCTGT CGGCTGACAA GTTTCCAGTA 
TCTCTGGATA GTTCTACCAA TAAAAATAAA GAACCAGGAA TGGAAAGATC TTCCCCTTCT 
AAATCCCAGT TGCCGGGTAA TAGGTGGTAT ATGCACAGCT CAAGGAGTCT TCAGGACAGA 
AACTGCCCAT CTCAAAAGAA GCGCATTAAT GTTGCTGACA TGCAGGAGCA ACAACTGGCT 
AAGTGTGAGG CCCAAGATTT AATGGGGTCT TATTTGCCGA GACAAGATCA AGAGGGAACC 
CCTTACCTGA AATCTGGAAT CAGCCTCTTC TCTCATGAAC CCGAGTCTGA TCCCTCTGAA 
GACAGAGCCC CAGAGCCAGC TCATGTTCAC AGCATGCCAC CCACAGCCTC TGCACTGAAA 
TTATCCCAAT TTCGAGTAGA AGAATCTACC AAGAATCCAG CTGCTGCTCA CATTACTAAT 
ACTACAGGGT GTAACCTGAG GGAAGAAAGT GTGAGCAAAG AGAAGCCAGA AGTGTTATCT 
TCAACAGAAA GAAGCAACAA AAGACTTTCT ATGGTGGCAT CAGGCTTGAC CCCAAAAGAA 
CTCATGCTTG TGCAGAAATT TGCCAGAAAA CATCACGTCA CTTTAACTAA TCTAATTACT 
GAAGAGACTA CTCATGTCAT TATGAAAACA GATCCTGAGT TTGTGTGTGA ACGGACACTG 
AAATATTTTC TGGGAATTGC AGGAGGAAAA TGGGTAGTTA GCTATTTTTG GGTCATCCAG 
TCTATTAAAG AAAGAAAGAT GCTGGATGAG CATGATTTTG AAGTCAGAGG AGATGTTGTC 
AATGGAAGAA ACCATCAAGG CCCAAAACGA GCAAGAGAAT CCCGGGACAA GAAGATCTTC 
AAGGGCCTGG AAATCTGTTG CTATGGACCT TTTACCAATA TGCCCACAGA TCAACTAGAA 
TGGATGGTGC AGCTGTGTGG GGCTTCTGTG GTGAAGGAGC CCTCATCATT CACCCCAGGC 
CAGGGTACTC ACCCAGTTGT GGTTGTGCAG CCGGATGCCT GGACAGAGGA TGCTGGCTTC 
CATGTGATCG GGCAGATGTG TGAAGCACCT GTGGTAACCC GAGAGTGGGT ACTGGACAGT 
GTGGCCCTTT ACCAGTGCCA GGAGCTGGAC ACCTACCTGG TCCCCCAGGT CGCCCAGAGC 
TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_012186166.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012041556.1 Ovis aries breast cancer 1 (BRCA1), transcript variant X6, mRNA.

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CloneID	OSm29450
Clone ID Related Accession (Same CDS sequence)	XM_012186166.1 , XM_012186165.2
Accession Version	XM_012186166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAAT GTCCATTGTG TAAGAATGAT 
ATAACCAAAA GAAGCCTACA AGAAAGTACA AGATTTAGTC AACTTGTTGA AGAGCTGTTG 
AAGATCATTC ATGCTTTTGA GCTTGACACA GGATTGCAGT TTGCAAACAG CTATAACTTT 
TCAAGAAAGG AAAATAACTC TCCTGAGCAT CTGAAGGAGG AAGTTTCTAT AATCCAAAGT 
ATGGGCTACC GGAACCGGGC CAAAAGACTT TGGCAGAGTG AACCTGAAAA TCCTACCTTG 
CAGGAAACCA GTCTTACTGT CAAACTCTCT AACCTTGGAA TTGTGAGATC TCTGAGGACA 
AAGCAGCAGA CACAATCTCA GAATAAGTCT GTCTACATTG AATTGGGATC TGATTCTTCT 
GAAGACACAG TTAATAAGGC CAGTTATCTC AGTGTGGGAG ACCATGAATT GTTAGAAATC 
ACCCCTCAAG GAGCCAAGGC CAAGACCAAT TTGAATCCTG CAAAAAAGGC TGCTTGTGAG 
TTTTCTGAGA AGGACATAAC AAATACTGAA CATCATCAAC TCAGTATTAA AGATCTGATC 
ACCACCCAGG AGCATGCAGC TGAGATGCAT GCAGCTGAGA CGCATCCAGA AAAGTATCAG 
GGTATTTCTG TTTCAGACTT TCACGTGGAG CCATGTGGCA CAGATACTCA TGCCAGCTCA 
TTACAGCATG AGAACAGCAG TTTATTGCTC ACTGAAAACA GACTGAATGT AGAAAAGGCT 
GAATTCTGTA ATAAAAGCAA ACAGCCTGTC TTAGTAAAGA GCCAACAGAG CAGATGGGCT 
GAAAGTAAGG GCACATGTAA GGATAGGCAG ATTCCCAGCA CTGGGAAAAA GATAGTTCTG 
AATACTGATC CCCTGTATGG AAGAAAAGAA CTGTGTAAGC AGAAACCTGC ATGCTCTGAC 
AGTCCTGGAG ATTCCCAAGA TGTTCCTTGG GTAACCCTGA ATAATAGCAT ACAGAAAGTT 
AATGACTGGT TTTCCAGAAG TGATGAAATA TTAACTTCTG ACGACTCGTG CGATGGGGGG 
TCTGAATCAA ATAATGAAGT AGCTGGTGCA GTGGAAATTC CAAATAACGT TGATGGATAT 
TCAGGTTCTT CAGAGAAAAT CGACTTAATG GCCAGTGATC CTCATGGTAC TTTAATACAT 
GAAAGAGTCC ACTCCAAACC AGTAGAGAGT AATATTGAAG ATAAAATATT TGGGAAAACC 
TATCGGAGGA AGTCGAGTCT CCCTAACTTC AACCACATAG CTGAAGATCT AATTATAGGT 
GCATTTACTG TAGAACCTCA GAAAACACAA GAGCAGCCCC TCACAAATAA GCTAAAATGT 
AAAAGGAGAG GTACATCAGG CCTTCAGCCT GAGGATTTTA TCAAGAAAGG TGATTTGACG 
ATTGTTCCAA AGACTCCTGA AAAGATGATT GAGAGAACTG ACCAAACAGA GCAGAAACAT 
CACGGGATAA ATATTACTAG TGATGGTCAT GAGAATAAAA CAAAACGTGA TGATGTTCAG 
AAAGAGCAAA ACGCTAACCC AACAGAATCA TTGGAAAACG AATCTGTTTT CAGAACTAAG 
GCTGAACCTA TAAACACCAG TATAAGCAAT ATGGAACTAG AATTGAATGT CCGCAGTTCA 
AAAGCACCTA AGAATAGGCT GAAGAGGAAA TCCTCTACCA GGAAAATTCC TGAACTTGAA 
CTAGTAGTCA GTAGAAATCC AAGTCCACCT AATCATACTG AGCTACTAAT TGATAGCAGT 
TCTAGCAATG AAGAGATGAA GAAAAAGCAT TCTAGCCAAA TGCCAGTCAG GCACAGCCAA 
AAGCTTCAAC TCATGGGAGA TAAAGAACCT ACAGCTGGAG CCAAGAAGAG TAACAAAACA 
TATGAACAAA TAAATAAAAG ACTTGCTAGT GATGCTTTTC CAGAACTAAA TTTTGCAAAC 
ACACCTGGTT ATTTTACTAA CTGTTCTAGT AAACCTGAAG AGTTTGTTCA TCCTAGCCTT 
CAAAGAGAGG AGAATCTAGG AACAATTCAA GTGTTGAATA ATACCAAAGA CCCCAAAGAT 
CTGATATTAA GAGGAGGAAA AGCTTTGCAA ATGGAAAGAT CTGTAGAGAA TACCAATATT 
TCCTTGGTTC CTGATACTGA TTATAGCACT CAGGATAGTA TCTCATTACT AGAAGCTAAA 
ACCCCAGAGA AGGCAAAGAC TGCACCAAAT CCATGTGTGA GTCTGTGTGC AGCAATCGAA 
AACCCCAAGG AACTTATCCA TAGGGATTTT AAAGATAACA AAAACAACAC AGAGGGCTTT 
CAGGGTCTCC TGGGACATGA CGTTAACTAT GTCATTCAGG AGACAAGAGG AGAAATGGAA 
GACAGTGAAC TTGATACACA GTATTTGCAG AATACGTTCA AGGTTTCAAA GCGTCAGACA 
TTTGCTCTGT TTTCCAATCC AGGAAATCCA CAAAAGGAAT GTGCCACAGT CTTTGCCCAC 
TCGGGGTCCT TAAAGGATCA AAGTCCAAGA GACCCCCTCA AATGCAGACA AAAAGAAGAC 
AGTCGGGGAA AGAGTGAGTC TAAAAGCCAG CATGTGCAGG CCGTTTGTAC AACAGTGCAC 
TTTCCTGTGG CTGGTCAGAA AGATAAGACG CCGGGTGACG ATGCCAAATG TAGTGCAAAA 
GAAGTCACTA GGGTTTGTCA GTCGTTACAA TTGAGAGGCC ACAAAACTGA ACTTGTTTTT 
GCTAATAAGC AAGGGGTTTC AGAAAAACCA AATCTAACAC CATCACTTTT TCCCATCAAG 
TCATCTGTTA AAACCGTATG TAAGAAAAGC CCATCAGAGA AATTTGAGGA ACCTGTAATG 
TCACCTGAAA GAACATTGGG AAGTGAGAGC ATCATTCAAA GTGCAGTGAG CACAATCAGC 
CAAAATGACA TTCAAGGAAG CACTTTTAAA GAAGTCAACT CAAGCAGTGT TAATGAAGTA 
GGTTCCAGTA CTAATGAAGT AGGCTCTAGT ATTAATGAAG TAGGTTCCAG TGGTGAAAAC 
ATTCAAGCAG AACCAGGTAG AAACAGAGAA CCTAAATTAA GAGCTTTACT CAGATTAGGT 
CTTATGCAAC CTGAAGTCTA TAAGCAAAGC CTTCCTGTAA GTAACTGTCA CCATCCTGAA 
ATAAAAAGGC AAGGAGAAAA TGAGGACATG CTTCAGGCTG TTAAGGCAGA TTTCTCCCCA 
TGTCTAATTT CAGATAACCT CGAACAACCT ATGGGAAGCT GTCATGCTTC TCAGGTTTGT 
TCTGAGACAC CTGACAACTT GTTAAATGAC GACGAAATAA AAGAAAATAG CCACTTTGCT 
GAAAGTGACA TTAAGGAAAG ATCTGCTGTT TTTAGTGAAA GTGTCCAAAA AGGAGAATTC 
AGAGGGAGCC CTGGCCCTTT CACCCATACA CATTTGGCTC AGGGTCACCA AAGAGGGGCT 
GGGAAACTAG AGTCAGCAGA GACTGTGTCT AGTGAGGATG AAGAGCTTCC CTGCTTCCAA 
CAATTACTGT TTGGTAAAGT AACCGATACA CTGTCTCCAT CTACTGGATG GAACACTATT 
GCCACTGAGG GTCTGTCTAA GGAAACAGAG GAGAACCTAG AATCATTGAA GAGTGGCTTA 
AATGACTGCA GTGGCCAGGG AACATCAGCC AAGGTATCCC AGGAACATCA CCTTAATGAG 
GAAGTGAGAT GTTCTGGTAG CTTATTTTCC TCACAGTACA GTGCAGTGGA AGACTTGACC 
ACTAATACAA ACACCCAGGA TCCTTTCTTG ATGTTTGAGC GTCCTTCCAA ACAAGTGTAT 
CAGTCTGAAA GCGAGGAAGT TCTGAGTGAC AAGGAATTTG TTTCAGATGA TGAAGAAAGG 
GAAACTGGCT TGGAAGAAGA TAGTTGCCAA GAAGAGCAGA GTGTGGATTC AGACTTAGGT 
GAAGCAGCAT CCGACCATGT GAGTGAAACA AGCCTCTCTG AAGACTGTGT GGGGCTGTCC 
TCTCAGAGTG ATATCTTAAC CACTCAGCAG AGGGATACCA TGCAAGATAA CCTTCTAAAG 
CTCCAGCAGG AAATGGCCGA GCTGGAAGCT GTGTTAGAGC AGCACGGGAG CCAGCCTTCC 
CACAGCTCTG CTTCCCTCAC AGCTGATTCT CGTGGCCCAG AGCACCTGCT AAATCTAGAG 
CAAGACACAT CAGAAAGAGC AATATTAACT TCAGAGAAAA GTAGAGACTG TTCTAGAAGC 
CAGAATCCTG AAAGCCTGTC GGCTGACAAG TTTCCAGTAT CTCTGGATAG TTCTACCAAT 
AAAAATAAAG AACCAGGAAT GGAAAGATCT TCCCCTTCTA AATCCCAGTT GCCGGGTAAT 
AGGTGGTATA TGCACAGCTC AAGGAGTCTT CAGGACAGAA ACTGCCCATC TCAAAAGAAG 
CGCATTAATG TTGCTGACAT GCAGGAGCAA CAACTGGCTA AGTGTGAGGC CCAAGATTTA 
ATGGGGTCTT ATTTGCCGAG ACAAGATCAA GAGGGAACCC CTTACCTGAA ATCTGGAATC 
AGCCTCTTCT CTCATGAACC CGAGTCTGAT CCCTCTGAAG ACAGAGCCCC AGAGCCAGCT 
CATGTTCACA GCATGCCACC CACAGCCTCT GCACTGAAAT TATCCCAATT TCGAGTAGAA 
GAATCTACCA AGAATCCAGC TGCTGCTCAC ATTACTAATA CTACAGGGTG TAACCTGAGG 
GAAGAAAGTG TGAGCAAAGA GAAGCCAGAA GTGTTATCTT CAACAGAAAG AAGCAACAAA 
AGACTTTCTA TGGTGGCATC AGGCTTGACC CCAAAAGAAC TCATGCTTGT GCAGAAATTT 
GCCAGAAAAC ATCACGTCAC TTTAACTAAT CTAATTACTG AAGAGACTAC TCATGTCATT 
ATGAAAACAG ATCCTGAGTT TGTGTGTGAA CGGACACTGA AATATTTTCT GGGAATTGCA 
GGAGGAAAAT GGGTAGTTAG CTATTTTTGG GTCATCCAGT CTATTAAAGA AAGAAAGATG 
CTGGATGAGC ATGATTTTGA AGTCAGAGGA GATGTTGTCA ATGGAAGAAA CCATCAAGGC 
CCAAAACGAG CAAGAGAATC CCGGGACAAG AAGATCTTCA AGGGCCTGGA AATCTGTTGC 
TATGGACCTT TTACCAATAT GCCCACAGAT CAACTAGAAT GGATGGTGCA GCTGTGTGGG 
GCTTCTGTGG TGAAGGAGCC CTCATCATTC ACCCCAGGCC AGGGTACTCA CCCAGTTGTG 
GTTGTGCAGC CGGATGCCTG GACAGAGGAT GCTGGCTTCC ATGTGATCGG GCAGATGTGT 
GAAGCACCTG TGGTAACCCG AGAGTGGGTA CTGGACAGTG TGGCCCTTTA CCAGTGCCAG 
GAGCTGGACA CCTACCTGGT CCCCCAGGTC GCCCAGAGCT GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012041556.1
CDS	119..5566
Translation	MLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESAETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_012186166.1
Organism	Ovis aries(sheep)
Definition	Ovis aries breast cancer 1 (BRCA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012186166.1

ORF Insert Sequence:

ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAAT GTCCATTGTG TAAGAATGAT 
ATAACCAAAA GAAGCCTACA AGAAAGTACA AGATTTAGTC AACTTGTTGA AGAGCTGTTG 
AAGATCATTC ATGCTTTTGA GCTTGACACA GGATTGCAGT TTGCAAACAG CTATAACTTT 
TCAAGAAAGG AAAATAACTC TCCTGAGCAT CTGAAGGAGG AAGTTTCTAT AATCCAAAGT 
ATGGGCTACC GGAACCGGGC CAAAAGACTT TGGCAGAGTG AACCTGAAAA TCCTACCTTG 
CAGGAAACCA GTCTTACTGT CAAACTCTCT AACCTTGGAA TTGTGAGATC TCTGAGGACA 
AAGCAGCAGA CACAATCTCA GAATAAGTCT GTCTACATTG AATTGGGATC TGATTCTTCT 
GAAGACACAG TTAATAAGGC CAGTTATCTC AGTGTGGGAG ACCATGAATT GTTAGAAATC 
ACCCCTCAAG GAGCCAAGGC CAAGACCAAT TTGAATCCTG CAAAAAAGGC TGCTTGTGAG 
TTTTCTGAGA AGGACATAAC AAATACTGAA CATCATCAAC TCAGTATTAA AGATCTGATC 
ACCACCCAGG AGCATGCAGC TGAGATGCAT GCAGCTGAGA CGCATCCAGA AAAGTATCAG 
GGTATTTCTG TTTCAGACTT TCACGTGGAG CCATGTGGCA CAGATACTCA TGCCAGCTCA 
TTACAGCATG AGAACAGCAG TTTATTGCTC ACTGAAAACA GACTGAATGT AGAAAAGGCT 
GAATTCTGTA ATAAAAGCAA ACAGCCTGTC TTAGTAAAGA GCCAACAGAG CAGATGGGCT 
GAAAGTAAGG GCACATGTAA GGATAGGCAG ATTCCCAGCA CTGGGAAAAA GATAGTTCTG 
AATACTGATC CCCTGTATGG AAGAAAAGAA CTGTGTAAGC AGAAACCTGC ATGCTCTGAC 
AGTCCTGGAG ATTCCCAAGA TGTTCCTTGG GTAACCCTGA ATAATAGCAT ACAGAAAGTT 
AATGACTGGT TTTCCAGAAG TGATGAAATA TTAACTTCTG ACGACTCGTG CGATGGGGGG 
TCTGAATCAA ATAATGAAGT AGCTGGTGCA GTGGAAATTC CAAATAACGT TGATGGATAT 
TCAGGTTCTT CAGAGAAAAT CGACTTAATG GCCAGTGATC CTCATGGTAC TTTAATACAT 
GAAAGAGTCC ACTCCAAACC AGTAGAGAGT AATATTGAAG ATAAAATATT TGGGAAAACC 
TATCGGAGGA AGTCGAGTCT CCCTAACTTC AACCACATAG CTGAAGATCT AATTATAGGT 
GCATTTACTG TAGAACCTCA GAAAACACAA GAGCAGCCCC TCACAAATAA GCTAAAATGT 
AAAAGGAGAG GTACATCAGG CCTTCAGCCT GAGGATTTTA TCAAGAAAGG TGATTTGACG 
ATTGTTCCAA AGACTCCTGA AAAGATGATT GAGAGAACTG ACCAAACAGA GCAGAAACAT 
CACGGGATAA ATATTACTAG TGATGGTCAT GAGAATAAAA CAAAACGTGA TGATGTTCAG 
AAAGAGCAAA ACGCTAACCC AACAGAATCA TTGGAAAACG AATCTGTTTT CAGAACTAAG 
GCTGAACCTA TAAACACCAG TATAAGCAAT ATGGAACTAG AATTGAATGT CCGCAGTTCA 
AAAGCACCTA AGAATAGGCT GAAGAGGAAA TCCTCTACCA GGAAAATTCC TGAACTTGAA 
CTAGTAGTCA GTAGAAATCC AAGTCCACCT AATCATACTG AGCTACTAAT TGATAGCAGT 
TCTAGCAATG AAGAGATGAA GAAAAAGCAT TCTAGCCAAA TGCCAGTCAG GCACAGCCAA 
AAGCTTCAAC TCATGGGAGA TAAAGAACCT ACAGCTGGAG CCAAGAAGAG TAACAAAACA 
TATGAACAAA TAAATAAAAG ACTTGCTAGT GATGCTTTTC CAGAACTAAA TTTTGCAAAC 
ACACCTGGTT ATTTTACTAA CTGTTCTAGT AAACCTGAAG AGTTTGTTCA TCCTAGCCTT 
CAAAGAGAGG AGAATCTAGG AACAATTCAA GTGTTGAATA ATACCAAAGA CCCCAAAGAT 
CTGATATTAA GAGGAGGAAA AGCTTTGCAA ATGGAAAGAT CTGTAGAGAA TACCAATATT 
TCCTTGGTTC CTGATACTGA TTATAGCACT CAGGATAGTA TCTCATTACT AGAAGCTAAA 
ACCCCAGAGA AGGCAAAGAC TGCACCAAAT CCATGTGTGA GTCTGTGTGC AGCAATCGAA 
AACCCCAAGG AACTTATCCA TAGGGATTTT AAAGATAACA AAAACAACAC AGAGGGCTTT 
CAGGGTCTCC TGGGACATGA CGTTAACTAT GTCATTCAGG AGACAAGAGG AGAAATGGAA 
GACAGTGAAC TTGATACACA GTATTTGCAG AATACGTTCA AGGTTTCAAA GCGTCAGACA 
TTTGCTCTGT TTTCCAATCC AGGAAATCCA CAAAAGGAAT GTGCCACAGT CTTTGCCCAC 
TCGGGGTCCT TAAAGGATCA AAGTCCAAGA GACCCCCTCA AATGCAGACA AAAAGAAGAC 
AGTCGGGGAA AGAGTGAGTC TAAAAGCCAG CATGTGCAGG CCGTTTGTAC AACAGTGCAC 
TTTCCTGTGG CTGGTCAGAA AGATAAGACG CCGGGTGACG ATGCCAAATG TAGTGCAAAA 
GAAGTCACTA GGGTTTGTCA GTCGTTACAA TTGAGAGGCC ACAAAACTGA ACTTGTTTTT 
GCTAATAAGC AAGGGGTTTC AGAAAAACCA AATCTAACAC CATCACTTTT TCCCATCAAG 
TCATCTGTTA AAACCGTATG TAAGAAAAGC CCATCAGAGA AATTTGAGGA ACCTGTAATG 
TCACCTGAAA GAACATTGGG AAGTGAGAGC ATCATTCAAA GTGCAGTGAG CACAATCAGC 
CAAAATGACA TTCAAGGAAG CACTTTTAAA GAAGTCAACT CAAGCAGTGT TAATGAAGTA 
GGTTCCAGTA CTAATGAAGT AGGCTCTAGT ATTAATGAAG TAGGTTCCAG TGGTGAAAAC 
ATTCAAGCAG AACCAGGTAG AAACAGAGAA CCTAAATTAA GAGCTTTACT CAGATTAGGT 
CTTATGCAAC CTGAAGTCTA TAAGCAAAGC CTTCCTGTAA GTAACTGTCA CCATCCTGAA 
ATAAAAAGGC AAGGAGAAAA TGAGGACATG CTTCAGGCTG TTAAGGCAGA TTTCTCCCCA 
TGTCTAATTT CAGATAACCT CGAACAACCT ATGGGAAGCT GTCATGCTTC TCAGGTTTGT 
TCTGAGACAC CTGACAACTT GTTAAATGAC GACGAAATAA AAGAAAATAG CCACTTTGCT 
GAAAGTGACA TTAAGGAAAG ATCTGCTGTT TTTAGTGAAA GTGTCCAAAA AGGAGAATTC 
AGAGGGAGCC CTGGCCCTTT CACCCATACA CATTTGGCTC AGGGTCACCA AAGAGGGGCT 
GGGAAACTAG AGTCAGCAGA GACTGTGTCT AGTGAGGATG AAGAGCTTCC CTGCTTCCAA 
CAATTACTGT TTGGTAAAGT AACCGATACA CTGTCTCCAT CTACTGGATG GAACACTATT 
GCCACTGAGG GTCTGTCTAA GGAAACAGAG GAGAACCTAG AATCATTGAA GAGTGGCTTA 
AATGACTGCA GTGGCCAGGG AACATCAGCC AAGGTATCCC AGGAACATCA CCTTAATGAG 
GAAGTGAGAT GTTCTGGTAG CTTATTTTCC TCACAGTACA GTGCAGTGGA AGACTTGACC 
ACTAATACAA ACACCCAGGA TCCTTTCTTG ATGTTTGAGC GTCCTTCCAA ACAAGTGTAT 
CAGTCTGAAA GCGAGGAAGT TCTGAGTGAC AAGGAATTTG TTTCAGATGA TGAAGAAAGG 
GAAACTGGCT TGGAAGAAGA TAGTTGCCAA GAAGAGCAGA GTGTGGATTC AGACTTAGGT 
GAAGCAGCAT CCGACCATGT GAGTGAAACA AGCCTCTCTG AAGACTGTGT GGGGCTGTCC 
TCTCAGAGTG ATATCTTAAC CACTCAGCAG AGGGATACCA TGCAAGATAA CCTTCTAAAG 
CTCCAGCAGG AAATGGCCGA GCTGGAAGCT GTGTTAGAGC AGCACGGGAG CCAGCCTTCC 
CACAGCTCTG CTTCCCTCAC AGCTGATTCT CGTGGCCCAG AGCACCTGCT AAATCTAGAG 
CAAGACACAT CAGAAAGAGC AATATTAACT TCAGAGAAAA GTAGAGACTG TTCTAGAAGC 
CAGAATCCTG AAAGCCTGTC GGCTGACAAG TTTCCAGTAT CTCTGGATAG TTCTACCAAT 
AAAAATAAAG AACCAGGAAT GGAAAGATCT TCCCCTTCTA AATCCCAGTT GCCGGGTAAT 
AGGTGGTATA TGCACAGCTC AAGGAGTCTT CAGGACAGAA ACTGCCCATC TCAAAAGAAG 
CGCATTAATG TTGCTGACAT GCAGGAGCAA CAACTGGCTA AGTGTGAGGC CCAAGATTTA 
ATGGGGTCTT ATTTGCCGAG ACAAGATCAA GAGGGAACCC CTTACCTGAA ATCTGGAATC 
AGCCTCTTCT CTCATGAACC CGAGTCTGAT CCCTCTGAAG ACAGAGCCCC AGAGCCAGCT 
CATGTTCACA GCATGCCACC CACAGCCTCT GCACTGAAAT TATCCCAATT TCGAGTAGAA 
GAATCTACCA AGAATCCAGC TGCTGCTCAC ATTACTAATA CTACAGGGTG TAACCTGAGG 
GAAGAAAGTG TGAGCAAAGA GAAGCCAGAA GTGTTATCTT CAACAGAAAG AAGCAACAAA 
AGACTTTCTA TGGTGGCATC AGGCTTGACC CCAAAAGAAC TCATGCTTGT GCAGAAATTT 
GCCAGAAAAC ATCACGTCAC TTTAACTAAT CTAATTACTG AAGAGACTAC TCATGTCATT 
ATGAAAACAG ATCCTGAGTT TGTGTGTGAA CGGACACTGA AATATTTTCT GGGAATTGCA 
GGAGGAAAAT GGGTAGTTAG CTATTTTTGG GTCATCCAGT CTATTAAAGA AAGAAAGATG 
CTGGATGAGC ATGATTTTGA AGTCAGAGGA GATGTTGTCA ATGGAAGAAA CCATCAAGGC 
CCAAAACGAG CAAGAGAATC CCGGGACAAG AAGATCTTCA AGGGCCTGGA AATCTGTTGC 
TATGGACCTT TTACCAATAT GCCCACAGAT CAACTAGAAT GGATGGTGCA GCTGTGTGGG 
GCTTCTGTGG TGAAGGAGCC CTCATCATTC ACCCCAGGCC AGGGTACTCA CCCAGTTGTG 
GTTGTGCAGC CGGATGCCTG GACAGAGGAT GCTGGCTTCC ATGTGATCGG GCAGATGTGT 
GAAGCACCTG TGGTAACCCG AGAGTGGGTA CTGGACAGTG TGGCCCTTTA CCAGTGCCAG 
GAGCTGGACA CCTACCTGGT CCCCCAGGTC GCCCAGAGCT GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_012186165.2 ) cDNA ORF clone, Ovis aries(sheep) -> XP_012041555.1 Ovis aries breast cancer 1 (BRCA1), transcript variant X5, mRNA.

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CloneID	OSm29450
Clone ID Related Accession (Same CDS sequence)	XM_012186166.1 , XM_012186165.2
Accession Version	XM_012186165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2015 this sequence version replaced XM_012186165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAAT GTCCATTGTG TAAGAATGAT 
ATAACCAAAA GAAGCCTACA AGAAAGTACA AGATTTAGTC AACTTGTTGA AGAGCTGTTG 
AAGATCATTC ATGCTTTTGA GCTTGACACA GGATTGCAGT TTGCAAACAG CTATAACTTT 
TCAAGAAAGG AAAATAACTC TCCTGAGCAT CTGAAGGAGG AAGTTTCTAT AATCCAAAGT 
ATGGGCTACC GGAACCGGGC CAAAAGACTT TGGCAGAGTG AACCTGAAAA TCCTACCTTG 
CAGGAAACCA GTCTTACTGT CAAACTCTCT AACCTTGGAA TTGTGAGATC TCTGAGGACA 
AAGCAGCAGA CACAATCTCA GAATAAGTCT GTCTACATTG AATTGGGATC TGATTCTTCT 
GAAGACACAG TTAATAAGGC CAGTTATCTC AGTGTGGGAG ACCATGAATT GTTAGAAATC 
ACCCCTCAAG GAGCCAAGGC CAAGACCAAT TTGAATCCTG CAAAAAAGGC TGCTTGTGAG 
TTTTCTGAGA AGGACATAAC AAATACTGAA CATCATCAAC TCAGTATTAA AGATCTGATC 
ACCACCCAGG AGCATGCAGC TGAGATGCAT GCAGCTGAGA CGCATCCAGA AAAGTATCAG 
GGTATTTCTG TTTCAGACTT TCACGTGGAG CCATGTGGCA CAGATACTCA TGCCAGCTCA 
TTACAGCATG AGAACAGCAG TTTATTGCTC ACTGAAAACA GACTGAATGT AGAAAAGGCT 
GAATTCTGTA ATAAAAGCAA ACAGCCTGTC TTAGTAAAGA GCCAACAGAG CAGATGGGCT 
GAAAGTAAGG GCACATGTAA GGATAGGCAG ATTCCCAGCA CTGGGAAAAA GATAGTTCTG 
AATACTGATC CCCTGTATGG AAGAAAAGAA CTGTGTAAGC AGAAACCTGC ATGCTCTGAC 
AGTCCTGGAG ATTCCCAAGA TGTTCCTTGG GTAACCCTGA ATAATAGCAT ACAGAAAGTT 
AATGACTGGT TTTCCAGAAG TGATGAAATA TTAACTTCTG ACGACTCGTG CGATGGGGGG 
TCTGAATCAA ATAATGAAGT AGCTGGTGCA GTGGAAATTC CAAATAACGT TGATGGATAT 
TCAGGTTCTT CAGAGAAAAT CGACTTAATG GCCAGTGATC CTCATGGTAC TTTAATACAT 
GAAAGAGTCC ACTCCAAACC AGTAGAGAGT AATATTGAAG ATAAAATATT TGGGAAAACC 
TATCGGAGGA AGTCGAGTCT CCCTAACTTC AACCACATAG CTGAAGATCT AATTATAGGT 
GCATTTACTG TAGAACCTCA GAAAACACAA GAGCAGCCCC TCACAAATAA GCTAAAATGT 
AAAAGGAGAG GTACATCAGG CCTTCAGCCT GAGGATTTTA TCAAGAAAGG TGATTTGACG 
ATTGTTCCAA AGACTCCTGA AAAGATGATT GAGAGAACTG ACCAAACAGA GCAGAAACAT 
CACGGGATAA ATATTACTAG TGATGGTCAT GAGAATAAAA CAAAACGTGA TGATGTTCAG 
AAAGAGCAAA ACGCTAACCC AACAGAATCA TTGGAAAACG AATCTGTTTT CAGAACTAAG 
GCTGAACCTA TAAACACCAG TATAAGCAAT ATGGAACTAG AATTGAATGT CCGCAGTTCA 
AAAGCACCTA AGAATAGGCT GAAGAGGAAA TCCTCTACCA GGAAAATTCC TGAACTTGAA 
CTAGTAGTCA GTAGAAATCC AAGTCCACCT AATCATACTG AGCTACTAAT TGATAGCAGT 
TCTAGCAATG AAGAGATGAA GAAAAAGCAT TCTAGCCAAA TGCCAGTCAG GCACAGCCAA 
AAGCTTCAAC TCATGGGAGA TAAAGAACCT ACAGCTGGAG CCAAGAAGAG TAACAAAACA 
TATGAACAAA TAAATAAAAG ACTTGCTAGT GATGCTTTTC CAGAACTAAA TTTTGCAAAC 
ACACCTGGTT ATTTTACTAA CTGTTCTAGT AAACCTGAAG AGTTTGTTCA TCCTAGCCTT 
CAAAGAGAGG AGAATCTAGG AACAATTCAA GTGTTGAATA ATACCAAAGA CCCCAAAGAT 
CTGATATTAA GAGGAGGAAA AGCTTTGCAA ATGGAAAGAT CTGTAGAGAA TACCAATATT 
TCCTTGGTTC CTGATACTGA TTATAGCACT CAGGATAGTA TCTCATTACT AGAAGCTAAA 
ACCCCAGAGA AGGCAAAGAC TGCACCAAAT CCATGTGTGA GTCTGTGTGC AGCAATCGAA 
AACCCCAAGG AACTTATCCA TAGGGATTTT AAAGATAACA AAAACAACAC AGAGGGCTTT 
CAGGGTCTCC TGGGACATGA CGTTAACTAT GTCATTCAGG AGACAAGAGG AGAAATGGAA 
GACAGTGAAC TTGATACACA GTATTTGCAG AATACGTTCA AGGTTTCAAA GCGTCAGACA 
TTTGCTCTGT TTTCCAATCC AGGAAATCCA CAAAAGGAAT GTGCCACAGT CTTTGCCCAC 
TCGGGGTCCT TAAAGGATCA AAGTCCAAGA GACCCCCTCA AATGCAGACA AAAAGAAGAC 
AGTCGGGGAA AGAGTGAGTC TAAAAGCCAG CATGTGCAGG CCGTTTGTAC AACAGTGCAC 
TTTCCTGTGG CTGGTCAGAA AGATAAGACG CCGGGTGACG ATGCCAAATG TAGTGCAAAA 
GAAGTCACTA GGGTTTGTCA GTCGTTACAA TTGAGAGGCC ACAAAACTGA ACTTGTTTTT 
GCTAATAAGC AAGGGGTTTC AGAAAAACCA AATCTAACAC CATCACTTTT TCCCATCAAG 
TCATCTGTTA AAACCGTATG TAAGAAAAGC CCATCAGAGA AATTTGAGGA ACCTGTAATG 
TCACCTGAAA GAACATTGGG AAGTGAGAGC ATCATTCAAA GTGCAGTGAG CACAATCAGC 
CAAAATGACA TTCAAGGAAG CACTTTTAAA GAAGTCAACT CAAGCAGTGT TAATGAAGTA 
GGTTCCAGTA CTAATGAAGT AGGCTCTAGT ATTAATGAAG TAGGTTCCAG TGGTGAAAAC 
ATTCAAGCAG AACCAGGTAG AAACAGAGAA CCTAAATTAA GAGCTTTACT CAGATTAGGT 
CTTATGCAAC CTGAAGTCTA TAAGCAAAGC CTTCCTGTAA GTAACTGTCA CCATCCTGAA 
ATAAAAAGGC AAGGAGAAAA TGAGGACATG CTTCAGGCTG TTAAGGCAGA TTTCTCCCCA 
TGTCTAATTT CAGATAACCT CGAACAACCT ATGGGAAGCT GTCATGCTTC TCAGGTTTGT 
TCTGAGACAC CTGACAACTT GTTAAATGAC GACGAAATAA AAGAAAATAG CCACTTTGCT 
GAAAGTGACA TTAAGGAAAG ATCTGCTGTT TTTAGTGAAA GTGTCCAAAA AGGAGAATTC 
AGAGGGAGCC CTGGCCCTTT CACCCATACA CATTTGGCTC AGGGTCACCA AAGAGGGGCT 
GGGAAACTAG AGTCAGCAGA GACTGTGTCT AGTGAGGATG AAGAGCTTCC CTGCTTCCAA 
CAATTACTGT TTGGTAAAGT AACCGATACA CTGTCTCCAT CTACTGGATG GAACACTATT 
GCCACTGAGG GTCTGTCTAA GGAAACAGAG GAGAACCTAG AATCATTGAA GAGTGGCTTA 
AATGACTGCA GTGGCCAGGG AACATCAGCC AAGGTATCCC AGGAACATCA CCTTAATGAG 
GAAGTGAGAT GTTCTGGTAG CTTATTTTCC TCACAGTACA GTGCAGTGGA AGACTTGACC 
ACTAATACAA ACACCCAGGA TCCTTTCTTG ATGTTTGAGC GTCCTTCCAA ACAAGTGTAT 
CAGTCTGAAA GCGAGGAAGT TCTGAGTGAC AAGGAATTTG TTTCAGATGA TGAAGAAAGG 
GAAACTGGCT TGGAAGAAGA TAGTTGCCAA GAAGAGCAGA GTGTGGATTC AGACTTAGGT 
GAAGCAGCAT CCGACCATGT GAGTGAAACA AGCCTCTCTG AAGACTGTGT GGGGCTGTCC 
TCTCAGAGTG ATATCTTAAC CACTCAGCAG AGGGATACCA TGCAAGATAA CCTTCTAAAG 
CTCCAGCAGG AAATGGCCGA GCTGGAAGCT GTGTTAGAGC AGCACGGGAG CCAGCCTTCC 
CACAGCTCTG CTTCCCTCAC AGCTGATTCT CGTGGCCCAG AGCACCTGCT AAATCTAGAG 
CAAGACACAT CAGAAAGAGC AATATTAACT TCAGAGAAAA GTAGAGACTG TTCTAGAAGC 
CAGAATCCTG AAAGCCTGTC GGCTGACAAG TTTCCAGTAT CTCTGGATAG TTCTACCAAT 
AAAAATAAAG AACCAGGAAT GGAAAGATCT TCCCCTTCTA AATCCCAGTT GCCGGGTAAT 
AGGTGGTATA TGCACAGCTC AAGGAGTCTT CAGGACAGAA ACTGCCCATC TCAAAAGAAG 
CGCATTAATG TTGCTGACAT GCAGGAGCAA CAACTGGCTA AGTGTGAGGC CCAAGATTTA 
ATGGGGTCTT ATTTGCCGAG ACAAGATCAA GAGGGAACCC CTTACCTGAA ATCTGGAATC 
AGCCTCTTCT CTCATGAACC CGAGTCTGAT CCCTCTGAAG ACAGAGCCCC AGAGCCAGCT 
CATGTTCACA GCATGCCACC CACAGCCTCT GCACTGAAAT TATCCCAATT TCGAGTAGAA 
GAATCTACCA AGAATCCAGC TGCTGCTCAC ATTACTAATA CTACAGGGTG TAACCTGAGG 
GAAGAAAGTG TGAGCAAAGA GAAGCCAGAA GTGTTATCTT CAACAGAAAG AAGCAACAAA 
AGACTTTCTA TGGTGGCATC AGGCTTGACC CCAAAAGAAC TCATGCTTGT GCAGAAATTT 
GCCAGAAAAC ATCACGTCAC TTTAACTAAT CTAATTACTG AAGAGACTAC TCATGTCATT 
ATGAAAACAG ATCCTGAGTT TGTGTGTGAA CGGACACTGA AATATTTTCT GGGAATTGCA 
GGAGGAAAAT GGGTAGTTAG CTATTTTTGG GTCATCCAGT CTATTAAAGA AAGAAAGATG 
CTGGATGAGC ATGATTTTGA AGTCAGAGGA GATGTTGTCA ATGGAAGAAA CCATCAAGGC 
CCAAAACGAG CAAGAGAATC CCGGGACAAG AAGATCTTCA AGGGCCTGGA AATCTGTTGC 
TATGGACCTT TTACCAATAT GCCCACAGAT CAACTAGAAT GGATGGTGCA GCTGTGTGGG 
GCTTCTGTGG TGAAGGAGCC CTCATCATTC ACCCCAGGCC AGGGTACTCA CCCAGTTGTG 
GTTGTGCAGC CGGATGCCTG GACAGAGGAT GCTGGCTTCC ATGTGATCGG GCAGATGTGT 
GAAGCACCTG TGGTAACCCG AGAGTGGGTA CTGGACAGTG TGGCCCTTTA CCAGTGCCAG 
GAGCTGGACA CCTACCTGGT CCCCCAGGTC GCCCAGAGCT GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012041555.1
CDS	210..5657
Translation	MLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLQETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESAETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_012186165.2
Organism	Ovis aries(sheep)
Definition	Ovis aries breast cancer 1 (BRCA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012186165.2

ORF Insert Sequence:

ATGCTGAAAC TTCTCAACCA GAAGAAAGGG CCTTCACAAT GTCCATTGTG TAAGAATGAT 
ATAACCAAAA GAAGCCTACA AGAAAGTACA AGATTTAGTC AACTTGTTGA AGAGCTGTTG 
AAGATCATTC ATGCTTTTGA GCTTGACACA GGATTGCAGT TTGCAAACAG CTATAACTTT 
TCAAGAAAGG AAAATAACTC TCCTGAGCAT CTGAAGGAGG AAGTTTCTAT AATCCAAAGT 
ATGGGCTACC GGAACCGGGC CAAAAGACTT TGGCAGAGTG AACCTGAAAA TCCTACCTTG 
CAGGAAACCA GTCTTACTGT CAAACTCTCT AACCTTGGAA TTGTGAGATC TCTGAGGACA 
AAGCAGCAGA CACAATCTCA GAATAAGTCT GTCTACATTG AATTGGGATC TGATTCTTCT 
GAAGACACAG TTAATAAGGC CAGTTATCTC AGTGTGGGAG ACCATGAATT GTTAGAAATC 
ACCCCTCAAG GAGCCAAGGC CAAGACCAAT TTGAATCCTG CAAAAAAGGC TGCTTGTGAG 
TTTTCTGAGA AGGACATAAC AAATACTGAA CATCATCAAC TCAGTATTAA AGATCTGATC 
ACCACCCAGG AGCATGCAGC TGAGATGCAT GCAGCTGAGA CGCATCCAGA AAAGTATCAG 
GGTATTTCTG TTTCAGACTT TCACGTGGAG CCATGTGGCA CAGATACTCA TGCCAGCTCA 
TTACAGCATG AGAACAGCAG TTTATTGCTC ACTGAAAACA GACTGAATGT AGAAAAGGCT 
GAATTCTGTA ATAAAAGCAA ACAGCCTGTC TTAGTAAAGA GCCAACAGAG CAGATGGGCT 
GAAAGTAAGG GCACATGTAA GGATAGGCAG ATTCCCAGCA CTGGGAAAAA GATAGTTCTG 
AATACTGATC CCCTGTATGG AAGAAAAGAA CTGTGTAAGC AGAAACCTGC ATGCTCTGAC 
AGTCCTGGAG ATTCCCAAGA TGTTCCTTGG GTAACCCTGA ATAATAGCAT ACAGAAAGTT 
AATGACTGGT TTTCCAGAAG TGATGAAATA TTAACTTCTG ACGACTCGTG CGATGGGGGG 
TCTGAATCAA ATAATGAAGT AGCTGGTGCA GTGGAAATTC CAAATAACGT TGATGGATAT 
TCAGGTTCTT CAGAGAAAAT CGACTTAATG GCCAGTGATC CTCATGGTAC TTTAATACAT 
GAAAGAGTCC ACTCCAAACC AGTAGAGAGT AATATTGAAG ATAAAATATT TGGGAAAACC 
TATCGGAGGA AGTCGAGTCT CCCTAACTTC AACCACATAG CTGAAGATCT AATTATAGGT 
GCATTTACTG TAGAACCTCA GAAAACACAA GAGCAGCCCC TCACAAATAA GCTAAAATGT 
AAAAGGAGAG GTACATCAGG CCTTCAGCCT GAGGATTTTA TCAAGAAAGG TGATTTGACG 
ATTGTTCCAA AGACTCCTGA AAAGATGATT GAGAGAACTG ACCAAACAGA GCAGAAACAT 
CACGGGATAA ATATTACTAG TGATGGTCAT GAGAATAAAA CAAAACGTGA TGATGTTCAG 
AAAGAGCAAA ACGCTAACCC AACAGAATCA TTGGAAAACG AATCTGTTTT CAGAACTAAG 
GCTGAACCTA TAAACACCAG TATAAGCAAT ATGGAACTAG AATTGAATGT CCGCAGTTCA 
AAAGCACCTA AGAATAGGCT GAAGAGGAAA TCCTCTACCA GGAAAATTCC TGAACTTGAA 
CTAGTAGTCA GTAGAAATCC AAGTCCACCT AATCATACTG AGCTACTAAT TGATAGCAGT 
TCTAGCAATG AAGAGATGAA GAAAAAGCAT TCTAGCCAAA TGCCAGTCAG GCACAGCCAA 
AAGCTTCAAC TCATGGGAGA TAAAGAACCT ACAGCTGGAG CCAAGAAGAG TAACAAAACA 
TATGAACAAA TAAATAAAAG ACTTGCTAGT GATGCTTTTC CAGAACTAAA TTTTGCAAAC 
ACACCTGGTT ATTTTACTAA CTGTTCTAGT AAACCTGAAG AGTTTGTTCA TCCTAGCCTT 
CAAAGAGAGG AGAATCTAGG AACAATTCAA GTGTTGAATA ATACCAAAGA CCCCAAAGAT 
CTGATATTAA GAGGAGGAAA AGCTTTGCAA ATGGAAAGAT CTGTAGAGAA TACCAATATT 
TCCTTGGTTC CTGATACTGA TTATAGCACT CAGGATAGTA TCTCATTACT AGAAGCTAAA 
ACCCCAGAGA AGGCAAAGAC TGCACCAAAT CCATGTGTGA GTCTGTGTGC AGCAATCGAA 
AACCCCAAGG AACTTATCCA TAGGGATTTT AAAGATAACA AAAACAACAC AGAGGGCTTT 
CAGGGTCTCC TGGGACATGA CGTTAACTAT GTCATTCAGG AGACAAGAGG AGAAATGGAA 
GACAGTGAAC TTGATACACA GTATTTGCAG AATACGTTCA AGGTTTCAAA GCGTCAGACA 
TTTGCTCTGT TTTCCAATCC AGGAAATCCA CAAAAGGAAT GTGCCACAGT CTTTGCCCAC 
TCGGGGTCCT TAAAGGATCA AAGTCCAAGA GACCCCCTCA AATGCAGACA AAAAGAAGAC 
AGTCGGGGAA AGAGTGAGTC TAAAAGCCAG CATGTGCAGG CCGTTTGTAC AACAGTGCAC 
TTTCCTGTGG CTGGTCAGAA AGATAAGACG CCGGGTGACG ATGCCAAATG TAGTGCAAAA 
GAAGTCACTA GGGTTTGTCA GTCGTTACAA TTGAGAGGCC ACAAAACTGA ACTTGTTTTT 
GCTAATAAGC AAGGGGTTTC AGAAAAACCA AATCTAACAC CATCACTTTT TCCCATCAAG 
TCATCTGTTA AAACCGTATG TAAGAAAAGC CCATCAGAGA AATTTGAGGA ACCTGTAATG 
TCACCTGAAA GAACATTGGG AAGTGAGAGC ATCATTCAAA GTGCAGTGAG CACAATCAGC 
CAAAATGACA TTCAAGGAAG CACTTTTAAA GAAGTCAACT CAAGCAGTGT TAATGAAGTA 
GGTTCCAGTA CTAATGAAGT AGGCTCTAGT ATTAATGAAG TAGGTTCCAG TGGTGAAAAC 
ATTCAAGCAG AACCAGGTAG AAACAGAGAA CCTAAATTAA GAGCTTTACT CAGATTAGGT 
CTTATGCAAC CTGAAGTCTA TAAGCAAAGC CTTCCTGTAA GTAACTGTCA CCATCCTGAA 
ATAAAAAGGC AAGGAGAAAA TGAGGACATG CTTCAGGCTG TTAAGGCAGA TTTCTCCCCA 
TGTCTAATTT CAGATAACCT CGAACAACCT ATGGGAAGCT GTCATGCTTC TCAGGTTTGT 
TCTGAGACAC CTGACAACTT GTTAAATGAC GACGAAATAA AAGAAAATAG CCACTTTGCT 
GAAAGTGACA TTAAGGAAAG ATCTGCTGTT TTTAGTGAAA GTGTCCAAAA AGGAGAATTC 
AGAGGGAGCC CTGGCCCTTT CACCCATACA CATTTGGCTC AGGGTCACCA AAGAGGGGCT 
GGGAAACTAG AGTCAGCAGA GACTGTGTCT AGTGAGGATG AAGAGCTTCC CTGCTTCCAA 
CAATTACTGT TTGGTAAAGT AACCGATACA CTGTCTCCAT CTACTGGATG GAACACTATT 
GCCACTGAGG GTCTGTCTAA GGAAACAGAG GAGAACCTAG AATCATTGAA GAGTGGCTTA 
AATGACTGCA GTGGCCAGGG AACATCAGCC AAGGTATCCC AGGAACATCA CCTTAATGAG 
GAAGTGAGAT GTTCTGGTAG CTTATTTTCC TCACAGTACA GTGCAGTGGA AGACTTGACC 
ACTAATACAA ACACCCAGGA TCCTTTCTTG ATGTTTGAGC GTCCTTCCAA ACAAGTGTAT 
CAGTCTGAAA GCGAGGAAGT TCTGAGTGAC AAGGAATTTG TTTCAGATGA TGAAGAAAGG 
GAAACTGGCT TGGAAGAAGA TAGTTGCCAA GAAGAGCAGA GTGTGGATTC AGACTTAGGT 
GAAGCAGCAT CCGACCATGT GAGTGAAACA AGCCTCTCTG AAGACTGTGT GGGGCTGTCC 
TCTCAGAGTG ATATCTTAAC CACTCAGCAG AGGGATACCA TGCAAGATAA CCTTCTAAAG 
CTCCAGCAGG AAATGGCCGA GCTGGAAGCT GTGTTAGAGC AGCACGGGAG CCAGCCTTCC 
CACAGCTCTG CTTCCCTCAC AGCTGATTCT CGTGGCCCAG AGCACCTGCT AAATCTAGAG 
CAAGACACAT CAGAAAGAGC AATATTAACT TCAGAGAAAA GTAGAGACTG TTCTAGAAGC 
CAGAATCCTG AAAGCCTGTC GGCTGACAAG TTTCCAGTAT CTCTGGATAG TTCTACCAAT 
AAAAATAAAG AACCAGGAAT GGAAAGATCT TCCCCTTCTA AATCCCAGTT GCCGGGTAAT 
AGGTGGTATA TGCACAGCTC AAGGAGTCTT CAGGACAGAA ACTGCCCATC TCAAAAGAAG 
CGCATTAATG TTGCTGACAT GCAGGAGCAA CAACTGGCTA AGTGTGAGGC CCAAGATTTA 
ATGGGGTCTT ATTTGCCGAG ACAAGATCAA GAGGGAACCC CTTACCTGAA ATCTGGAATC 
AGCCTCTTCT CTCATGAACC CGAGTCTGAT CCCTCTGAAG ACAGAGCCCC AGAGCCAGCT 
CATGTTCACA GCATGCCACC CACAGCCTCT GCACTGAAAT TATCCCAATT TCGAGTAGAA 
GAATCTACCA AGAATCCAGC TGCTGCTCAC ATTACTAATA CTACAGGGTG TAACCTGAGG 
GAAGAAAGTG TGAGCAAAGA GAAGCCAGAA GTGTTATCTT CAACAGAAAG AAGCAACAAA 
AGACTTTCTA TGGTGGCATC AGGCTTGACC CCAAAAGAAC TCATGCTTGT GCAGAAATTT 
GCCAGAAAAC ATCACGTCAC TTTAACTAAT CTAATTACTG AAGAGACTAC TCATGTCATT 
ATGAAAACAG ATCCTGAGTT TGTGTGTGAA CGGACACTGA AATATTTTCT GGGAATTGCA 
GGAGGAAAAT GGGTAGTTAG CTATTTTTGG GTCATCCAGT CTATTAAAGA AAGAAAGATG 
CTGGATGAGC ATGATTTTGA AGTCAGAGGA GATGTTGTCA ATGGAAGAAA CCATCAAGGC 
CCAAAACGAG CAAGAGAATC CCGGGACAAG AAGATCTTCA AGGGCCTGGA AATCTGTTGC 
TATGGACCTT TTACCAATAT GCCCACAGAT CAACTAGAAT GGATGGTGCA GCTGTGTGGG 
GCTTCTGTGG TGAAGGAGCC CTCATCATTC ACCCCAGGCC AGGGTACTCA CCCAGTTGTG 
GTTGTGCAGC CGGATGCCTG GACAGAGGAT GCTGGCTTCC ATGTGATCGG GCAGATGTGT 
GAAGCACCTG TGGTAACCCG AGAGTGGGTA CTGGACAGTG TGGCCCTTTA CCAGTGCCAG 
GAGCTGGACA CCTACCTGGT CCCCCAGGTC GCCCAGAGCT GCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRCA1 ( XM_015098806.1 ) cDNA ORF clone, Ovis aries(sheep) -> XP_014954292.1 Ovis aries breast cancer 1 (BRCA1), transcript variant X4, mRNA.

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CloneID	OSm51575
Clone ID Related Accession (Same CDS sequence)	XM_015098806.1
Accession Version	XM_015098806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-16
Organism	Ovis aries(sheep)
Product	breast cancer type 1 susceptibility protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014639020.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ovis aries Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GGAAACCAGT CTTACTGTCA AACTCTCTAA CCTTGGAATT 
GTGAGATCTC TGAGGACAAA GCAGCAGACA CAATCTCAGA ATAAGTCTGT CTACATTGAA 
TTGGGATCTG ATTCTTCTGA AGACACAGTT AATAAGGCCA GTTATCTCAG TGTGGGAGAC 
CATGAATTGT TAGAAATCAC CCCTCAAGGA GCCAAGGCCA AGACCAATTT GAATCCTGCA 
AAAAAGGCTG CTTGTGAGTT TTCTGAGAAG GACATAACAA ATACTGAACA TCATCAACTC 
AGTATTAAAG ATCTGATCAC CACCCAGGAG CATGCAGCTG AGATGCATGC AGCTGAGACG 
CATCCAGAAA AGTATCAGGG TATTTCTGTT TCAGACTTTC ACGTGGAGCC ATGTGGCACA 
GATACTCATG CCAGCTCATT ACAGCATGAG AACAGCAGTT TATTGCTCAC TGAAAACAGA 
CTGAATGTAG AAAAGGCTGA ATTCTGTAAT AAAAGCAAAC AGCCTGTCTT AGTAAAGAGC 
CAACAGAGCA GATGGGCTGA AAGTAAGGGC ACATGTAAGG ATAGGCAGAT TCCCAGCACT 
GGGAAAAAGA TAGTTCTGAA TACTGATCCC CTGTATGGAA GAAAAGAACT GTGTAAGCAG 
AAACCTGCAT GCTCTGACAG TCCTGGAGAT TCCCAAGATG TTCCTTGGGT AACCCTGAAT 
AATAGCATAC AGAAAGTTAA TGACTGGTTT TCCAGAAGTG ATGAAATATT AACTTCTGAC 
GACTCGTGCG ATGGGGGGTC TGAATCAAAT AATGAAGTAG CTGGTGCAGT GGAAATTCCA 
AATAACGTTG ATGGATATTC AGGTTCTTCA GAGAAAATCG ACTTAATGGC CAGTGATCCT 
CATGGTACTT TAATACATGA AAGAGTCCAC TCCAAACCAG TAGAGAGTAA TATTGAAGAT 
AAAATATTTG GGAAAACCTA TCGGAGGAAG TCGAGTCTCC CTAACTTCAA CCACATAGCT 
GAAGATCTAA TTATAGGTGC ATTTACTGTA GAACCTCAGA AAACACAAGA GCAGCCCCTC 
ACAAATAAGC TAAAATGTAA AAGGAGAGGT ACATCAGGCC TTCAGCCTGA GGATTTTATC 
AAGAAAGGTG ATTTGACGAT TGTTCCAAAG ACTCCTGAAA AGATGATTGA GAGAACTGAC 
CAAACAGAGC AGAAACATCA CGGGATAAAT ATTACTAGTG ATGGTCATGA GAATAAAACA 
AAACGTGATG ATGTTCAGAA AGAGCAAAAC GCTAACCCAA CAGAATCATT GGAAAACGAA 
TCTGTTTTCA GAACTAAGGC TGAACCTATA AACACCAGTA TAAGCAATAT GGAACTAGAA 
TTGAATGTCC GCAGTTCAAA AGCACCTAAG AATAGGCTGA AGAGGAAATC CTCTACCAGG 
AAAATTCCTG AACTTGAACT AGTAGTCAGT AGAAATCCAA GTCCACCTAA TCATACTGAG 
CTACTAATTG ATAGCAGTTC TAGCAATGAA GAGATGAAGA AAAAGCATTC TAGCCAAATG 
CCAGTCAGGC ACAGCCAAAA GCTTCAACTC ATGGGAGATA AAGAACCTAC AGCTGGAGCC 
AAGAAGAGTA ACAAAACATA TGAACAAATA AATAAAAGAC TTGCTAGTGA TGCTTTTCCA 
GAACTAAATT TTGCAAACAC ACCTGGTTAT TTTACTAACT GTTCTAGTAA ACCTGAAGAG 
TTTGTTCATC CTAGCCTTCA AAGAGAGGAG AATCTAGGAA CAATTCAAGT GTTGAATAAT 
ACCAAAGACC CCAAAGATCT GATATTAAGA GGAGGAAAAG CTTTGCAAAT GGAAAGATCT 
GTAGAGAATA CCAATATTTC CTTGGTTCCT GATACTGATT ATAGCACTCA GGATAGTATC 
TCATTACTAG AAGCTAAAAC CCCAGAGAAG GCAAAGACTG CACCAAATCC ATGTGTGAGT 
CTGTGTGCAG CAATCGAAAA CCCCAAGGAA CTTATCCATA GGGATTTTAA AGATAACAAA 
AACAACACAG AGGGCTTTCA GGGTCTCCTG GGACATGACG TTAACTATGT CATTCAGGAG 
ACAAGAGGAG AAATGGAAGA CAGTGAACTT GATACACAGT ATTTGCAGAA TACGTTCAAG 
GTTTCAAAGC GTCAGACATT TGCTCTGTTT TCCAATCCAG GAAATCCACA AAAGGAATGT 
GCCACAGTCT TTGCCCACTC GGGGTCCTTA AAGGATCAAA GTCCAAGAGA CCCCCTCAAA 
TGCAGACAAA AAGAAGACAG TCGGGGAAAG AGTGAGTCTA AAAGCCAGCA TGTGCAGGCC 
GTTTGTACAA CAGTGCACTT TCCTGTGGCT GGTCAGAAAG ATAAGACGCC GGGTGACGAT 
GCCAAATGTA GTGCAAAAGA AGTCACTAGG GTTTGTCAGT CGTTACAATT GAGAGGCCAC 
AAAACTGAAC TTGTTTTTGC TAATAAGCAA GGGGTTTCAG AAAAACCAAA TCTAACACCA 
TCACTTTTTC CCATCAAGTC ATCTGTTAAA ACCGTATGTA AGAAAAGCCC ATCAGAGAAA 
TTTGAGGAAC CTGTAATGTC ACCTGAAAGA ACATTGGGAA GTGAGAGCAT CATTCAAAGT 
GCAGTGAGCA CAATCAGCCA AAATGACATT CAAGGAAGCA CTTTTAAAGA AGTCAACTCA 
AGCAGTGTTA ATGAAGTAGG TTCCAGTACT AATGAAGTAG GCTCTAGTAT TAATGAAGTA 
GGTTCCAGTG GTGAAAACAT TCAAGCAGAA CCAGGTAGAA ACAGAGAACC TAAATTAAGA 
GCTTTACTCA GATTAGGTCT TATGCAACCT GAAGTCTATA AGCAAAGCCT TCCTGTAAGT 
AACTGTCACC ATCCTGAAAT AAAAAGGCAA GGAGAAAATG AGGACATGCT TCAGGCTGTT 
AAGGCAGATT TCTCCCCATG TCTAATTTCA GATAACCTCG AACAACCTAT GGGAAGCTGT 
CATGCTTCTC AGGTTTGTTC TGAGACACCT GACAACTTGT TAAATGACGA CGAAATAAAA 
GAAAATAGCC ACTTTGCTGA AAGTGACATT AAGGAAAGAT CTGCTGTTTT TAGTGAAAGT 
GTCCAAAAAG GAGAATTCAG AGGGAGCCCT GGCCCTTTCA CCCATACACA TTTGGCTCAG 
GGTCACCAAA GAGGGGCTGG GAAACTAGAG TCAGCAGAGA CTGTGTCTAG TGAGGATGAA 
GAGCTTCCCT GCTTCCAACA ATTACTGTTT GGTAAAGTAA CCGATACACT GTCTCCATCT 
ACTGGATGGA ACACTATTGC CACTGAGGGT CTGTCTAAGG AAACAGAGGA GAACCTAGAA 
TCATTGAAGA GTGGCTTAAA TGACTGCAGT GGCCAGGGAA CATCAGCCAA GGTATCCCAG 
GAACATCACC TTAATGAGGA AGTGAGATGT TCTGGTAGCT TATTTTCCTC ACAGTACAGT 
GCAGTGGAAG ACTTGACCAC TAATACAAAC ACCCAGGATC CTTTCTTGAT GTTTGAGCGT 
CCTTCCAAAC AAGTGTATCA GTCTGAAAGC GAGGAAGTTC TGAGTGACAA GGAATTTGTT 
TCAGATGATG AAGAAAGGGA AACTGGCTTG GAAGAAGATA GTTGCCAAGA AGAGCAGAGT 
GTGGATTCAG ACTTAGGTGA AGCAGCATCC GACCATGTGA GTGAAACAAG CCTCTCTGAA 
GACTGTGTGG GGCTGTCCTC TCAGAGTGAT ATCTTAACCA CTCAGCAGAG GGATACCATG 
CAAGATAACC TTCTAAAGCT CCAGCAGGAA ATGGCCGAGC TGGAAGCTGT GTTAGAGCAG 
CACGGGAGCC AGCCTTCCCA CAGCTCTGCT TCCCTCACAG CTGATTCTCG TGGCCCAGAG 
CACCTGCTAA ATCTAGAGCA AGACACATCA GAAAGAGCAA TATTAACTTC AGAGAAAAGT 
AGAGACTGTT CTAGAAGCCA GAATCCTGAA AGCCTGTCGG CTGACAAGTT TCCAGTATCT 
CTGGATAGTT CTACCAATAA AAATAAAGAA CCAGGAATGG AAAGATCTTC CCCTTCTAAA 
TCCCAGTTGC CGGGTAATAG GTGGTATATG CACAGCTCAA GGAGTCTTCA GGACAGAAAC 
TGCCCATCTC AAAAGAAGCG CATTAATGTT GCTGACATGC AGGAGCAACA ACTGGCTAAG 
TGTGAGGCCC AAGATTTAAT GGGGTCTTAT TTGCCGAGAC AAGATCAAGA GGGAACCCCT 
TACCTGAAAT CTGGAATCAG CCTCTTCTCT CATGAACCCG AGTCTGATCC CTCTGAAGAC 
AGAGCCCCAG AGCCAGCTCA TGTTCACAGC ATGCCACCCA CAGCCTCTGC ACTGAAATTA 
TCCCAATTTC GAGTAGAAGA ATCTACCAAG AATCCAGCTG CTGCTCACAT TACTAATACT 
ACAGGGTGTA ACCTGAGGGA AGAAAGTGTG AGCAAAGAGA AGCCAGAAGT GTTATCTTCA 
ACAGAAAGAA GCAACAAAAG ACTTTCTATG GTGGCATCAG GCTTGACCCC AAAAGAACTC 
ATGCTTGTGC AGAAATTTGC CAGAAAACAT CACGTCACTT TAACTAATCT AATTACTGAA 
GAGACTACTC ATGTCATTAT GAAAACAGAT CCTGAGTTTG TGTGTGAACG GACACTGAAA 
TATTTTCTGG GAATTGCAGG AGGAAAATGG GTAGTTAGCT ATTTTTGGGT CATCCAGTCT 
ATTAAAGAAA GAAAGATGCT GGATGAGCAT GATTTTGAAG TCAGAGGAGA TGTTGTCAAT 
GGAAGAAACC ATCAAGGCCC AAAACGAGCA AGAGAATCCC GGGACAAGAA GATCTTCAAG 
GGCCTGGAAA TCTGTTGCTA TGGACCTTTT ACCAATATGC CCACAGATCA ACTAGAATGG 
ATGGTGCAGC TGTGTGGGGC TTCTGTGGTG AAGGAGCCCT CATCATTCAC CCCAGGCCAG 
GGTACTCACC CAGTTGTGGT TGTGCAGCCG GATGCCTGGA CAGAGGATGC TGGCTTCCAT 
GTGATCGGGC AGATGTGTGA AGCACCTGTG GTAACCCGAG AGTGGGTACT GGACAGTGTG 
GCCCTTTACC AGTGCCAGGA GCTGGACACC TACCTGGTCC CCCAGGTCGC CCAGAGCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014954292.1
CDS	213..5798
Translation	MDLSADHVEEVQNVLNAMQKILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNDITKRSLQESTRFSQLVEELLKIIHAFELDTGLQFANSYNFSRKENNSPEHLKEEVSIIQSMGYRNRAKRLWQSEPENPTLETSLTVKLSNLGIVRSLRTKQQTQSQNKSVYIELGSDSSEDTVNKASYLSVGDHELLEITPQGAKAKTNLNPAKKAACEFSEKDITNTEHHQLSIKDLITTQEHAAEMHAAETHPEKYQGISVSDFHVEPCGTDTHASSLQHENSSLLLTENRLNVEKAEFCNKSKQPVLVKSQQSRWAESKGTCKDRQIPSTGKKIVLNTDPLYGRKELCKQKPACSDSPGDSQDVPWVTLNNSIQKVNDWFSRSDEILTSDDSCDGGSESNNEVAGAVEIPNNVDGYSGSSEKIDLMASDPHGTLIHERVHSKPVESNIEDKIFGKTYRRKSSLPNFNHIAEDLIIGAFTVEPQKTQEQPLTNKLKCKRRGTSGLQPEDFIKKGDLTIVPKTPEKMIERTDQTEQKHHGINITSDGHENKTKRDDVQKEQNANPTESLENESVFRTKAEPINTSISNMELELNVRSSKAPKNRLKRKSSTRKIPELELVVSRNPSPPNHTELLIDSSSSNEEMKKKHSSQMPVRHSQKLQLMGDKEPTAGAKKSNKTYEQINKRLASDAFPELNFANTPGYFTNCSSKPEEFVHPSLQREENLGTIQVLNNTKDPKDLILRGGKALQMERSVENTNISLVPDTDYSTQDSISLLEAKTPEKAKTAPNPCVSLCAAIENPKELIHRDFKDNKNNTEGFQGLLGHDVNYVIQETRGEMEDSELDTQYLQNTFKVSKRQTFALFSNPGNPQKECATVFAHSGSLKDQSPRDPLKCRQKEDSRGKSESKSQHVQAVCTTVHFPVAGQKDKTPGDDAKCSAKEVTRVCQSLQLRGHKTELVFANKQGVSEKPNLTPSLFPIKSSVKTVCKKSPSEKFEEPVMSPERTLGSESIIQSAVSTISQNDIQGSTFKEVNSSSVNEVGSSTNEVGSSINEVGSSGENIQAEPGRNREPKLRALLRLGLMQPEVYKQSLPVSNCHHPEIKRQGENEDMLQAVKADFSPCLISDNLEQPMGSCHASQVCSETPDNLLNDDEIKENSHFAESDIKERSAVFSESVQKGEFRGSPGPFTHTHLAQGHQRGAGKLESAETVSSEDEELPCFQQLLFGKVTDTLSPSTGWNTIATEGLSKETEENLESLKSGLNDCSGQGTSAKVSQEHHLNEEVRCSGSLFSSQYSAVEDLTTNTNTQDPFLMFERPSKQVYQSESEEVLSDKEFVSDDEERETGLEEDSCQEEQSVDSDLGEAASDHVSETSLSEDCVGLSSQSDILTTQQRDTMQDNLLKLQQEMAELEAVLEQHGSQPSHSSASLTADSRGPEHLLNLEQDTSERAILTSEKSRDCSRSQNPESLSADKFPVSLDSSTNKNKEPGMERSSPSKSQLPGNRWYMHSSRSLQDRNCPSQKKRINVADMQEQQLAKCEAQDLMGSYLPRQDQEGTPYLKSGISLFSHEPESDPSEDRAPEPAHVHSMPPTASALKLSQFRVEESTKNPAAAHITNTTGCNLREESVSKEKPEVLSSTERSNKRLSMVASGLTPKELMLVQKFARKHHVTLTNLITEETTHVIMKTDPEFVCERTLKYFLGIAGGKWVVSYFWVIQSIKERKMLDEHDFEVRGDVVNGRNHQGPKRARESRDKKIFKGLEICCYGPFTNMPTDQLEWMVQLCGASVVKEPSSFTPGQGTHPVVVVQPDAWTEDAGFHVIGQMCEAPVVTREWVLDSVALYQCQELDTYLVPQVAQSCC

Target ORF information:

RefSeq Version	XM_015098806.1
Organism	Ovis aries(sheep)
Definition	Ovis aries breast cancer 1 (BRCA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015098806.1

ORF Insert Sequence:

ATGGATTTAT CTGCGGATCA TGTTGAAGAA GTACAAAATG TCCTTAATGC TATGCAGAAA 
ATCTTAGAGT GTCCAATATG TCTGGAGTTG ATCAAAGAGC CTGTCTCTAC AAAGTGTGAC 
CACATATTTT GCAAATTTTG TATGCTGAAA CTTCTCAACC AGAAGAAAGG GCCTTCACAA 
TGTCCATTGT GTAAGAATGA TATAACCAAA AGAAGCCTAC AAGAAAGTAC AAGATTTAGT 
CAACTTGTTG AAGAGCTGTT GAAGATCATT CATGCTTTTG AGCTTGACAC AGGATTGCAG 
TTTGCAAACA GCTATAACTT TTCAAGAAAG GAAAATAACT CTCCTGAGCA TCTGAAGGAG 
GAAGTTTCTA TAATCCAAAG TATGGGCTAC CGGAACCGGG CCAAAAGACT TTGGCAGAGT 
GAACCTGAAA ATCCTACCTT GGAAACCAGT CTTACTGTCA AACTCTCTAA CCTTGGAATT 
GTGAGATCTC TGAGGACAAA GCAGCAGACA CAATCTCAGA ATAAGTCTGT CTACATTGAA 
TTGGGATCTG ATTCTTCTGA AGACACAGTT AATAAGGCCA GTTATCTCAG TGTGGGAGAC 
CATGAATTGT TAGAAATCAC CCCTCAAGGA GCCAAGGCCA AGACCAATTT GAATCCTGCA 
AAAAAGGCTG CTTGTGAGTT TTCTGAGAAG GACATAACAA ATACTGAACA TCATCAACTC 
AGTATTAAAG ATCTGATCAC CACCCAGGAG CATGCAGCTG AGATGCATGC AGCTGAGACG 
CATCCAGAAA AGTATCAGGG TATTTCTGTT TCAGACTTTC ACGTGGAGCC ATGTGGCACA 
GATACTCATG CCAGCTCATT ACAGCATGAG AACAGCAGTT TATTGCTCAC TGAAAACAGA 
CTGAATGTAG AAAAGGCTGA ATTCTGTAAT AAAAGCAAAC AGCCTGTCTT AGTAAAGAGC 
CAACAGAGCA GATGGGCTGA AAGTAAGGGC ACATGTAAGG ATAGGCAGAT TCCCAGCACT 
GGGAAAAAGA TAGTTCTGAA TACTGATCCC CTGTATGGAA GAAAAGAACT GTGTAAGCAG 
AAACCTGCAT GCTCTGACAG TCCTGGAGAT TCCCAAGATG TTCCTTGGGT AACCCTGAAT 
AATAGCATAC AGAAAGTTAA TGACTGGTTT TCCAGAAGTG ATGAAATATT AACTTCTGAC 
GACTCGTGCG ATGGGGGGTC TGAATCAAAT AATGAAGTAG CTGGTGCAGT GGAAATTCCA 
AATAACGTTG ATGGATATTC AGGTTCTTCA GAGAAAATCG ACTTAATGGC CAGTGATCCT 
CATGGTACTT TAATACATGA AAGAGTCCAC TCCAAACCAG TAGAGAGTAA TATTGAAGAT 
AAAATATTTG GGAAAACCTA TCGGAGGAAG TCGAGTCTCC CTAACTTCAA CCACATAGCT 
GAAGATCTAA TTATAGGTGC ATTTACTGTA GAACCTCAGA AAACACAAGA GCAGCCCCTC 
ACAAATAAGC TAAAATGTAA AAGGAGAGGT ACATCAGGCC TTCAGCCTGA GGATTTTATC 
AAGAAAGGTG ATTTGACGAT TGTTCCAAAG ACTCCTGAAA AGATGATTGA GAGAACTGAC 
CAAACAGAGC AGAAACATCA CGGGATAAAT ATTACTAGTG ATGGTCATGA GAATAAAACA 
AAACGTGATG ATGTTCAGAA AGAGCAAAAC GCTAACCCAA CAGAATCATT GGAAAACGAA 
TCTGTTTTCA GAACTAAGGC TGAACCTATA AACACCAGTA TAAGCAATAT GGAACTAGAA 
TTGAATGTCC GCAGTTCAAA AGCACCTAAG AATAGGCTGA AGAGGAAATC CTCTACCAGG 
AAAATTCCTG AACTTGAACT AGTAGTCAGT AGAAATCCAA GTCCACCTAA TCATACTGAG 
CTACTAATTG ATAGCAGTTC TAGCAATGAA GAGATGAAGA AAAAGCATTC TAGCCAAATG 
CCAGTCAGGC ACAGCCAAAA GCTTCAACTC ATGGGAGATA AAGAACCTAC AGCTGGAGCC 
AAGAAGAGTA ACAAAACATA TGAACAAATA AATAAAAGAC TTGCTAGTGA TGCTTTTCCA 
GAACTAAATT TTGCAAACAC ACCTGGTTAT TTTACTAACT GTTCTAGTAA ACCTGAAGAG 
TTTGTTCATC CTAGCCTTCA AAGAGAGGAG AATCTAGGAA CAATTCAAGT GTTGAATAAT 
ACCAAAGACC CCAAAGATCT GATATTAAGA GGAGGAAAAG CTTTGCAAAT GGAAAGATCT 
GTAGAGAATA CCAATATTTC CTTGGTTCCT GATACTGATT ATAGCACTCA GGATAGTATC 
TCATTACTAG AAGCTAAAAC CCCAGAGAAG GCAAAGACTG CACCAAATCC ATGTGTGAGT 
CTGTGTGCAG CAATCGAAAA CCCCAAGGAA CTTATCCATA GGGATTTTAA AGATAACAAA 
AACAACACAG AGGGCTTTCA GGGTCTCCTG GGACATGACG TTAACTATGT CATTCAGGAG 
ACAAGAGGAG AAATGGAAGA CAGTGAACTT GATACACAGT ATTTGCAGAA TACGTTCAAG 
GTTTCAAAGC GTCAGACATT TGCTCTGTTT TCCAATCCAG GAAATCCACA AAAGGAATGT 
GCCACAGTCT TTGCCCACTC GGGGTCCTTA AAGGATCAAA GTCCAAGAGA CCCCCTCAAA 
TGCAGACAAA AAGAAGACAG TCGGGGAAAG AGTGAGTCTA AAAGCCAGCA TGTGCAGGCC 
GTTTGTACAA CAGTGCACTT TCCTGTGGCT GGTCAGAAAG ATAAGACGCC GGGTGACGAT 
GCCAAATGTA GTGCAAAAGA AGTCACTAGG GTTTGTCAGT CGTTACAATT GAGAGGCCAC 
AAAACTGAAC TTGTTTTTGC TAATAAGCAA GGGGTTTCAG AAAAACCAAA TCTAACACCA 
TCACTTTTTC CCATCAAGTC ATCTGTTAAA ACCGTATGTA AGAAAAGCCC ATCAGAGAAA 
TTTGAGGAAC CTGTAATGTC ACCTGAAAGA ACATTGGGAA GTGAGAGCAT CATTCAAAGT 
GCAGTGAGCA CAATCAGCCA AAATGACATT CAAGGAAGCA CTTTTAAAGA AGTCAACTCA 
AGCAGTGTTA ATGAAGTAGG TTCCAGTACT AATGAAGTAG GCTCTAGTAT TAATGAAGTA 
GGTTCCAGTG GTGAAAACAT TCAAGCAGAA CCAGGTAGAA ACAGAGAACC TAAATTAAGA 
GCTTTACTCA GATTAGGTCT TATGCAACCT GAAGTCTATA AGCAAAGCCT TCCTGTAAGT 
AACTGTCACC ATCCTGAAAT AAAAAGGCAA GGAGAAAATG AGGACATGCT TCAGGCTGTT 
AAGGCAGATT TCTCCCCATG TCTAATTTCA GATAACCTCG AACAACCTAT GGGAAGCTGT 
CATGCTTCTC AGGTTTGTTC TGAGACACCT GACAACTTGT TAAATGACGA CGAAATAAAA 
GAAAATAGCC ACTTTGCTGA AAGTGACATT AAGGAAAGAT CTGCTGTTTT TAGTGAAAGT 
GTCCAAAAAG GAGAATTCAG AGGGAGCCCT GGCCCTTTCA CCCATACACA TTTGGCTCAG 
GGTCACCAAA GAGGGGCTGG GAAACTAGAG TCAGCAGAGA CTGTGTCTAG TGAGGATGAA 
GAGCTTCCCT GCTTCCAACA ATTACTGTTT GGTAAAGTAA CCGATACACT GTCTCCATCT 
ACTGGATGGA ACACTATTGC CACTGAGGGT CTGTCTAAGG AAACAGAGGA GAACCTAGAA 
TCATTGAAGA GTGGCTTAAA TGACTGCAGT GGCCAGGGAA CATCAGCCAA GGTATCCCAG 
GAACATCACC TTAATGAGGA AGTGAGATGT TCTGGTAGCT TATTTTCCTC ACAGTACAGT 
GCAGTGGAAG ACTTGACCAC TAATACAAAC ACCCAGGATC CTTTCTTGAT GTTTGAGCGT 
CCTTCCAAAC AAGTGTATCA GTCTGAAAGC GAGGAAGTTC TGAGTGACAA GGAATTTGTT 
TCAGATGATG AAGAAAGGGA AACTGGCTTG GAAGAAGATA GTTGCCAAGA AGAGCAGAGT 
GTGGATTCAG ACTTAGGTGA AGCAGCATCC GACCATGTGA GTGAAACAAG CCTCTCTGAA 
GACTGTGTGG GGCTGTCCTC TCAGAGTGAT ATCTTAACCA CTCAGCAGAG GGATACCATG 
CAAGATAACC TTCTAAAGCT CCAGCAGGAA ATGGCCGAGC TGGAAGCTGT GTTAGAGCAG 
CACGGGAGCC AGCCTTCCCA CAGCTCTGCT TCCCTCACAG CTGATTCTCG TGGCCCAGAG 
CACCTGCTAA ATCTAGAGCA AGACACATCA GAAAGAGCAA TATTAACTTC AGAGAAAAGT 
AGAGACTGTT CTAGAAGCCA GAATCCTGAA AGCCTGTCGG CTGACAAGTT TCCAGTATCT 
CTGGATAGTT CTACCAATAA AAATAAAGAA CCAGGAATGG AAAGATCTTC CCCTTCTAAA 
TCCCAGTTGC CGGGTAATAG GTGGTATATG CACAGCTCAA GGAGTCTTCA GGACAGAAAC 
TGCCCATCTC AAAAGAAGCG CATTAATGTT GCTGACATGC AGGAGCAACA ACTGGCTAAG 
TGTGAGGCCC AAGATTTAAT GGGGTCTTAT TTGCCGAGAC AAGATCAAGA GGGAACCCCT 
TACCTGAAAT CTGGAATCAG CCTCTTCTCT CATGAACCCG AGTCTGATCC CTCTGAAGAC 
AGAGCCCCAG AGCCAGCTCA TGTTCACAGC ATGCCACCCA CAGCCTCTGC ACTGAAATTA 
TCCCAATTTC GAGTAGAAGA ATCTACCAAG AATCCAGCTG CTGCTCACAT TACTAATACT 
ACAGGGTGTA ACCTGAGGGA AGAAAGTGTG AGCAAAGAGA AGCCAGAAGT GTTATCTTCA 
ACAGAAAGAA GCAACAAAAG ACTTTCTATG GTGGCATCAG GCTTGACCCC AAAAGAACTC 
ATGCTTGTGC AGAAATTTGC CAGAAAACAT CACGTCACTT TAACTAATCT AATTACTGAA 
GAGACTACTC ATGTCATTAT GAAAACAGAT CCTGAGTTTG TGTGTGAACG GACACTGAAA 
TATTTTCTGG GAATTGCAGG AGGAAAATGG GTAGTTAGCT ATTTTTGGGT CATCCAGTCT 
ATTAAAGAAA GAAAGATGCT GGATGAGCAT GATTTTGAAG TCAGAGGAGA TGTTGTCAAT 
GGAAGAAACC ATCAAGGCCC AAAACGAGCA AGAGAATCCC GGGACAAGAA GATCTTCAAG 
GGCCTGGAAA TCTGTTGCTA TGGACCTTTT ACCAATATGC CCACAGATCA ACTAGAATGG 
ATGGTGCAGC TGTGTGGGGC TTCTGTGGTG AAGGAGCCCT CATCATTCAC CCCAGGCCAG 
GGTACTCACC CAGTTGTGGT TGTGCAGCCG GATGCCTGGA CAGAGGATGC TGGCTTCCAT 
GTGATCGGGC AGATGTGTGA AGCACCTGTG GTAACCCGAG AGTGGGTACT GGACAGTGTG 
GCCCTTTACC AGTGCCAGGA GCTGGACACC TACCTGGTCC CCCAGGTCGC CCAGAGCTGC 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	BRCA1
Entrez Gene ID	101108584
Full Name	BRCA1, DNA repair associated
Gene Type	protein-coding
Organism	Ovis aries(sheep)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

BRCA1 cDNA ORF clone, Ovis aries(sheep)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

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