BOH78_2869 cDNA ORF clone, Pichia kudriavzevii(Candida krusei)

Gene
Clones
gRNAs

Summary

Gene Symbol	BOH78_2869
Entrez Gene ID	31693465
Full Name	[Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial
Gene Type	protein-coding
Organism	Pichia kudriavzevii(Candida krusei)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020688667.1	XP_020544079.1	[Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following BOH78_2869 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BOH78_2869 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPi15765	XM_020688667.1 Latest version!	Pichia kudriavzevii [Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial (BOH78_2869), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BOH78_2869 ( XM_020688667.1 ) cDNA ORF clone, Pichia kudriavzevii(Candida krusei) -> XP_020544079.1 Pichia kudriavzevii [Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial (BOH78_2869), partial mRNA.

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CloneID	OPi15765
Clone ID Related Accession (Same CDS sequence)	XM_020688667.1
Accession Version	XM_020688667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-27
Organism	Pichia kudriavzevii(Candida krusei)
Product	[Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_018150445). COMPLETENESS: incomplete on both ends.

ATGACATTGC AAGGAAGTGG CAACGAATAT TACAGTGGGG AGACGTCTAG AGGTCCATCT 
TCCAGGTGGA GCGGTGACCA CAAAGGGGGG TTGCCGGGCA CAGACGCGGA TACGGACATG 
GACACTGCGA TGGATACCGG TCTGGGGGGT TTGGGCGACA GTCTGGGAAG CAGTAGATAC 
GACGACTTAA ATAGAAGTAG AGATAGAAGT AGAGATAGAA GCAGAAGCAG AAGCAGAAGC 
GCCAGTGTTG ATAGAAGTCT CCATGAGGGA AGAAGTATGG GATACGGAGG CGAGCGGTCA 
TACCGATCCA GAGACGAAAC TCCGAGGGCT ACATTGTACG TTAGAGGCTT CGTTACGGGG 
GTTAGGGCCA AGACGCTTGC CGATGTCTTT GAGAGGTACG GTCCAGTTGC ACAGGTGGAT 
GTGTTACCGC CACGAAGGTC AGACGGTGAG CAGTATGCGT TTGTTGCCTT TAGAAGTATT 
TCTGCCATGA ATGCAGTATT GGACGATATT GAGAGCGGGA GGCCGATCGA TGGCGGGGTG 
GCCATTGACC CCAAAGTTGG TCTTTTATGC GAGAGGGCCA GGAGTATGGA TACCTTTATG 
ACGTTTATCA ATGGGTCCCA CTCTTCAAAG AAGTTTGGAA CGGCTACCGA AGACCCAATC 
ATTGCCGGGA TGGCCAAATA CAACAACACC AATGATAAGA CGCCTCTGAG TAGTACCAGT 
GTCTCCATCT GTGGGCAGCT TTTCGAGACA GAGGCAGACG AAGTACTCAG TGAGTTTTTA 
AACCCACAGT TCTTAAAACA AGAAGACCAG GGCGACTATG AAACGCCCAG CGATCCCAAG 
CTAAGTACCA GCGGAACTAA GATGGAAACA AGTGGCACTC CCGACTCTGT TTGCACGCAG 
ACTACTGAAA CTATGAGGGT TTTGCCCAAT ACTCCCACAC CTAGTGCACC GTCCCCAACC 
ACTGTTGCCT TCAATGAGCA GCTCAAGATG TTTGAAGACT CAAAGGAGGA ATTGATGAAT 
ATTAAGTTTG GGCTTAATAA CGAGGCCAAA TACATTCACC ACTCCAATGT CGGGCTCCAA 
TACACAACCT TACCTGTAAC AGAACACCCT GAAGACCCTG ATGTAACTGC CACATATTTG 
CCCATGGAAT ATACGGAAGC GTTCCTAAGG GACTCTTCAG AGTTTCCATA CAAAATGTCC 
ATTGCTGACC TACCTGCACA ATCCAGAGTT GAAACCCAGA TAAAGCTCGA GCTTTCTGTC 
TCTCCTCCAC CTAAACAGTT TTTGTTGCAT CTGCCGAGGG ACACCATCAC AAAGCCAAAA 
TTCACATTGG CGGAAAATAC GCTTCCTGAA TCTATCAAAA AACACCTCCT TTATTTGGAG 
ATGTTTGTGA TTGGATCCGA AAGTATATCT TCCAAGGATG GCAGACTGAA GAGTTGCAAT 
GTTTGTAAAC GGTGTATGAG AAGAGAATTG AAACGGGCAT CTAGGAGGAA AGCCGGTTTG 
GTTGATGATT CTTCAAACTG GGATCTTAAC TTGCCCAAGA GAGCATTGAT AATAAACAGT 
AAAGAAATTG TTTCTTTCCC CTCCCCCAGT GGTAATGAGG TTGCTCGAAA GTTGGAGCTG 
CTATCTAGAA TTGTCTGTTA TTGCAGGCAC CATCAAGAGT TAGATGGCTT TAGGATTGCG 
ATTTTTTTGA AAAATGAACA AGATGAAATC ATAGGAAAAT GTCATACAAC ACCTATTTTG 
ATTATGGATA GGAAGAAATC TTCAAAATCT AGCAACTCAA ACAATACCGG TGCTCTCGTC 
TCCAACAAAG AACATCTTTC AGCTTCAAAG AACTCAATCA CCATTTCACA GGAGTCGTCA 
AGTGAATTTA GTATGGCAAC GTCCATATCT TCTACGAAAT TGAATCCTAA ACTAGTGACA 
AAACCTTTGG AAACTGATAT AATGATGGCG CGTGATAGCA GCAACAGCTC AATGGGTGTC 
CCTAAAACCA ACATTATGAG TATTTTGTCT TTAAAGGATG GGAAGAATAA CACCCATGGA 
AATAAGCCTC CAAAAACTGG AAGGGGAATG TTTTCTTCCA TCTTCTCCTT TGACCCTTCC 
AACTTATTGG GCGATGAATC TTCAAGGCAA ACTAAAAGAC AAAAGAGATT GTGGTCACAG 
TCCGATTCTG GAAGCTATCA CGATTCTATT AACCAACAAA GGTATCACTC GCAATCTGAA 
GCAAACAAAG GCAATGTACA AGATACCCCG GCTATGAATA GCGGGCATAT CATGCAAATC 
AAAAAAGAGG CCATTTCGCC TCTTAGCTCA GATGCTGTAT CACCGCATAA CCACATGCTG 
TCATCGGCAA CTAGCATATT TTCAGCTACG CCTGAAAGGA GGATATATGA GGGGCAGAAG 
ATGAACCTAA CATCGTCTTT CAACGATGAG TTAGCAATGA ATGTTGAAAA TATTGATAAT 
ATTGGTTCCA ATAGTGGTGT TTTAGACTTG AATGGTTTGG ATCTTGACGT TGACATGGAA 
ATGAATGTGA ATCTTAATCT TGGTGAACCA GTTCCAACAA TTCGAAAAAT TATACCTGCA 
CAGGGTTCCA TCAGAGGTGG TATTGAAGTC ACGCTTTTGG GTTCCAACTT TAAGCCGGGA 
TTAGAAGTGA AATTTGGTAG TAACATTTCT CTAGCTACGC AATGTTGGTC TGATTCCACT 
ATTGTTACGT ATTTACCTCC TGCACCACAA GCAGGGCCAG TACTAGTGAC TTTTGATTCT 
TCTGACATTG CTGCCAATAA TTCAGTTCCG CATCAAATAT TTACCTACAC CGATGATACT 
GATAAGCAAC TAATCGAGTT GGCACTCCAG ATCGTCGGTC TTAAGATGAA TGGAAAATTA 
GAGGATGCCA AGAATATTGC TAAAAGAATT GTTGGCAATA ATCAGATGGA CGATCCATCA 
TCAACCTCAT TTGGTCAAGG TGACCAATCC AATTCTTCAT CTACGTCCAA CCAATTGAAC 
CAACAACTAC AGTTGAATTG GATGGTGGTT GCTTCGAACA AAATTAAAGA ATTATCAACG 
TCTACGTTAA ACCATGAGGA CATTCTAATC AAATTCTTGA ATATCCTTCA GATCCCAAAT 
TACATTATAA CGAGTCCTAA CTGGGGAGTC TGCAATTCTG AGGGGCAAAC TATGCTCCAT 
TTGGCATGTT TGAAAAATTA TTCCAAGCTA ACTAATTTTC TTTTGAAAAG TGGCTCTAGA 
GTTGATTATA GGGACAAGAA TGGGTTTACG CCTGTGCACT TTGCCTTTAT TCCAGGAAAC 
AGAAAGATCA TCACGATGCT GATGAAATAC AACGCCAATG TCTCCAATAA ACTTGATAAT 
GGTATTTTGT TGAGTAGCAT TGCCGACTCT AATGTTCTTG ATTTGATTGA CGGACACGTT 
GACGTGAGTA GGAGGTCGTC AGACTCGACC TCTTACGAAG ACCTTGAAAT CGATGATACG 
TTTGATGACG AAATGGAGGC TTGTGGTATG GATGAAATTG GGTTCAATAG TGCATACAAA 
TCGAGCTCTA AAACGCATTA TACATCTTTG TATCGTAACA GGAAATCACA GTATGCAAGA 
AAGAAACGTG TGGATGATTC TGAAGCAGAT GATGAGGAAG AAGATGTTGA TCTGGACATG 
GATTTGGACG GTTCCGACGC TAGTACCGAC AAATCATCGT CAGGCACTAG CTCTATTAAA 
TCATTGGATC GAAAGAAAAA CACCAACTCT TTTGGTTTAA ACTTATGGAT TGCAATGAGG 
GAAGCAATAA CTAGTAAGGT CATTGAGTTG AGTGATGGTC AACATAAACA TGGTGGCAGG 
AAACATTCAC GTCCAATTCA TGGTACAAAT GTTGAAACGA GCCAACATCT CGACAAAACT 
AATGAAGCAA ATGATAATGA TGGTGAGGAG GGAGATGCAT TACCGACGTA CGACGATTTG 
TTTCCTAACG GTGCTTCTCT AAGAGCATTT ATCAATTTCC GGGGACACAC TGATGGTGAA 
GATGATGAAA AGCTATCCAA AACCAACAGA AGTGCAGAAC TAGAGGAAGA TGTTGGAGAT 
AGTGATGTGG ATATAATCGC AAAAAAGATG CACACATCCA TCACCTCAGA CACCAAGCTT 
ATGTTCTTTT GGTTGCCATT TATGCTTTTA CTATTATTGG TTGTTATTGG TGTGAAGATG 
AATATGATTT CTTTGAACAG TTTACAGTTG ATAAAGATTA GAGAAAGTGT CGGATCCTTT 
ATGCTTGGTA AGGATCGGTT CACCAACTTA CTAAACGAAA ACATCAACTA CGGACGTGAA 
CGGATGGAGA ATTTACTGCA CGATGTCAAT GGTGTATCAA CTCAATTGGC AATGTCTCAG 
TGGAAGATAA GCGAGGCACT TAGACAACAG ATATACAAAT ATGCAAAGCT GCCGCAGACA 
GGAGTCTCAC TTCGACAAAT GGTCCAATTT GGACCAAGAC CCAATCAAGG AACACTCTTT 
CACGCTTCAT ATTTTGCAGT ACATGAACTC CCCATTAGAC TAAGTCATCG TGTTCGGGAA 
TTGGAGAATT TACCGCATGG GTTGAGTGAA GAACCTTCGA TTAAATTAGT CAGAGACTGG 
TATGCAATCA GTTTTGAAGA ATTGATGGCT GTTAAGGAAC CCAATTTAAC TGAGGAAATG 
AAGGAGATTT TGTTGAAAGA CAGGAAAGAT GAATTTGGCT GGGAGAAATC ACCTGCTGAC 
CATGAGAGGC GACGCGCAGC GAAAAGGCGC AAAGAGTTCA TATTTGAAGA TGACGGTATT 
GTCATTGATA GAAGAAATAA GGATTCAGCG CTTGATAAGG ACGATAGTGC ATCCATTATT 
GAAGTAGAAG ATACGAAAAG CCATGAAGAA ACCGAATTCG AGAAGCCTAT TGGACTAAAA 
ACATATTTCC AAAGGTGTCC TAATGACATT GTTTATCCAA AAGAAGTTAT AGATTACAAT 
ATCAACGTCA CAAAGGTATT AACGAAAATC AAGAGAAGAC ATGACGCCAC TGTTTCTACA 
TTGGCTACCG GTGTCCAAAA TTGGAAACAA AAGCAAAATC ATAAATACTT GAACAGTCCG 
ATAAATCAGT TTTTAGATAG ATTTTATATG AGCAGAATCG GTATTAGAAT GTTAATTGGC 
CAAACGATAG CACTTAATCA ACAAGCACAA AGAAACATGC AAAATGACGA CTATGTCGGT 
ATAATTTGTC TGCACTGTAA CATTAAAGAA ATTATTGAAG ATGCTGTTGA CGCAGCACGC 
TTTGCATGCG AAGAACATTT TAATACAATG GAAGTTCCCA AAGTCCAACT ATATTGTCCT 
TCAGATTTGA CCTTCAGATA TGTTCCTGGA CATTTGGTTC ACATGCTATT CGAGACTCTC 
AAGAATAGTC TAAGAGCAAC AATTGAACAT CATTCCAAAT TGAATCCAGG CGTACCTCTT 
GATGATATCG AGTATCCACC AATCAAAATC ATTGTTGCCA AGGGGGATGA AGATATAACC 
ATCAAGATTT CCGATGAAGG TGGAGGAATT CCAAGATCTG CAATCCCACT AGTTTGGACT 
TATTTCTATA CCTCGGCAGA GAAACAAATA AAAGAAGACG ATATGCTCGA CGTTACCAAT 
AACTATAGAG CTCCATTTAT GGGGCTAGGC GTTGGGTTAC CACATTCTCG TTTGTATGCT 
AGATACTTCT CTGGTGACTT AAAATTGATT AGTATGGAGG GTTACGGAAC AGACTCAGAG 
AAGTATTTTG GTAAAATGAT ACTCTGTGCA AGGGAGCTAT CCTCCAGACG ACTTCCATTG 
CGGTTTGGGC TATGTTATTT GCAGAAGAGA GAACAATCGA GACTTCCTGC CATATCGAAA 
TTCGAAAATG ATTTGTTCAA TACATTCACA AAAGAACATC ACCATGGAAT GGCGCAGTCT 
TGTGACGACC AGCAATTATT TGCAGATTCA CAATTTCAGA TGTTGAAAAT ATCCGAACAT 
GGATCATTAA TTCAGCATGA AGAAAATACA CATGATTATC TGTTCAATGA TCAATGTATG 
TTTGACGAGG AGGAATTAAA TTTACGAAAT ACGTTTCAAG AAAAGGATAC AGAGATAAAT 
TTCATTAGAA ATATGCCAAA ATATTCACGG TCGAAAAACC CAAACGAAAC CAAACCCTTT 
AGGTTAATTA AACTGTCGAA TGATGCTAAA ATAGAAAGCA GTTCAATTGA CGATTTTTTT 
TGTCGAGTTA TAGGAAATGA AGCCGCTGTA AAGGATTACC ATTACCAGAT GCCTGTTTTC 
CCCACTGAAT TGACCAACTA TTCTTTAAAA CATTACTTGC GACAAGTTAA TGTCATCGAT 
TTAAATTATG ATATACATGG GTTTGAGAAA CTGATCTTTT TTCAGATACT GAAAATCTGT 
CGAAATATCA GCCGGTTTGA TGGAATACTG GATATTGTGG TACTTCATGA ACTGATTAAA 
TTTCTAGGTA AATATGGCAT GATACGATCT ATATTCAAAG TCATTGATGC TTTTGAACAT 
TTGGGGATTC ATCCAAATAA AGACACTCTT CATTTGCTGA TATACAAACT AAATGACATT 
AAAGGTCTAC GATGGAGAAA GGATCTTCTC AATTTCTATG TTCTTCTTGG TACCCGAAAA 
TGGGGGGTAT CGCCGGACAA AACGACAGAG GTACTTTCGG CAATACAAAG CCCTCCTGGA 
AGACCCCGGC TGGCTATTAC CAAACGACTA ATCAATGAAG GAATTGATGA CTCGTATTTC 
CAAAACACTA TGTTTCACGA TTATGTCACT ATAGAAATTG AAAGCCGTGA ACTCCCGTAT 
TTCGAGAGAT TCTACCATTT TTTACTTAAA AGAAAAGCAA TCACAGATAC CCATGAAAAC 
AAAATAATAT TATTCAACAT GTACATAGAA AAACTAGCAT CTTGCTGCAA ATTGAGGCAT 
GCAATTGAGG AAATGAAGAC GCATGAAGAG CTAACCACGC CGGATACATA CACTGCATTA 
ATTGACCAAA CAATCAAGAA TAATGTTTGG ACAGGGTTTG CCCTTCTCAA TCATTTAATG 
AGCCAAGGAA ACGTCCGTGT TTCCGATATA GGCATGGCGA TAATCGGCAA CTACCGTACT 
ATTCAGGAAA ACTTTACTAA GCAAGCGCAA AATGTTAATA GACTACACCA GCACAACATG 
CCACAAATAT ACAACATTGT CATTAAGATG TTACTGGCTA AATGTGGATA TCAAATAAGC 
GGCAACAACA AGTTCCTTCT ACCGGTGGAG AGAGACCTAC CACTAGATGA GGAGACGGTT 
ATATCTGACT TCCAAAAGAT TCAGTTTTGG AACTTAGATA CGGAATACTC ACACTACAAT 
GGCCTCTTGA CGTCTCGCTG TGGCGGATCA TGGATATTCA AACTCTGGGA TGTTATGGAT 
ATCGAGTGTG CACTTAGAGC CTACAGGTTG CGTTTATTGT TCCATCGAGT AGAGATGTTG 
AGACAAAGTG TGAGATTCTT TGGTAGCACC CGTGCAGTAC TTCAGTCTTC CTGTAAAGAA 
GGCACCCCAA TCAACTTGAA TATCTACAAG GCGGGCAAGC CAATTGTTGC AAAGAAGGAC 
GAGGAATACC CCGACTGGCT GTGGGGTTTA CTGGACAATG ACCTGCAAAT GGAAAACTTG 
AAGAAGGAAG ACTGGTTCAG ATATAATAGG AAACTCATCA AGAGACAAAA TGTCCAACGT 
ATAAAGATGA ACAACTTCAT GAACAACATG AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020544079.1
CDS	1..7776
Translation	MTLQGSGNEYYSGETSRGPSSRWSGDHKGGLPGTDADTDMDTAMDTGLGGLGDSLGSSRYDDLNRSRDRSRDRSRSRSRSASVDRSLHEGRSMGYGGERSYRSRDETPRATLYVRGFVTGVRAKTLADVFERYGPVAQVDVLPPRRSDGEQYAFVAFRSISAMNAVLDDIESGRPIDGGVAIDPKVGLLCERARSMDTFMTFINGSHSSKKFGTATEDPIIAGMAKYNNTNDKTPLSSTSVSICGQLFETEADEVLSEFLNPQFLKQEDQGDYETPSDPKLSTSGTKMETSGTPDSVCTQTTETMRVLPNTPTPSAPSPTTVAFNEQLKMFEDSKEELMNIKFGLNNEAKYIHHSNVGLQYTTLPVTEHPEDPDVTATYLPMEYTEAFLRDSSEFPYKMSIADLPAQSRVETQIKLELSVSPPPKQFLLHLPRDTITKPKFTLAENTLPESIKKHLLYLEMFVIGSESISSKDGRLKSCNVCKRCMRRELKRASRRKAGLVDDSSNWDLNLPKRALIINSKEIVSFPSPSGNEVARKLELLSRIVCYCRHHQELDGFRIAIFLKNEQDEIIGKCHTTPILIMDRKKSSKSSNSNNTGALVSNKEHLSASKNSITISQESSSEFSMATSISSTKLNPKLVTKPLETDIMMARDSSNSSMGVPKTNIMSILSLKDGKNNTHGNKPPKTGRGMFSSIFSFDPSNLLGDESSRQTKRQKRLWSQSDSGSYHDSINQQRYHSQSEANKGNVQDTPAMNSGHIMQIKKEAISPLSSDAVSPHNHMLSSATSIFSATPERRIYEGQKMNLTSSFNDELAMNVENIDNIGSNSGVLDLNGLDLDVDMEMNVNLNLGEPVPTIRKIIPAQGSIRGGIEVTLLGSNFKPGLEVKFGSNISLATQCWSDSTIVTYLPPAPQAGPVLVTFDSSDIAANNSVPHQIFTYTDDTDKQLIELALQIVGLKMNGKLEDAKNIAKRIVGNNQMDDPSSTSFGQGDQSNSSSTSNQLNQQLQLNWMVVASNKIKELSTSTLNHEDILIKFLNILQIPNYIITSPNWGVCNSEGQTMLHLACLKNYSKLTNFLLKSGSRVDYRDKNGFTPVHFAFIPGNRKIITMLMKYNANVSNKLDNGILLSSIADSNVLDLIDGHVDVSRRSSDSTSYEDLEIDDTFDDEMEACGMDEIGFNSAYKSSSKTHYTSLYRNRKSQYARKKRVDDSEADDEEEDVDLDMDLDGSDASTDKSSSGTSSIKSLDRKKNTNSFGLNLWIAMREAITSKVIELSDGQHKHGGRKHSRPIHGTNVETSQHLDKTNEANDNDGEEGDALPTYDDLFPNGASLRAFINFRGHTDGEDDEKLSKTNRSAELEEDVGDSDVDIIAKKMHTSITSDTKLMFFWLPFMLLLLLVVIGVKMNMISLNSLQLIKIRESVGSFMLGKDRFTNLLNENINYGRERMENLLHDVNGVSTQLAMSQWKISEALRQQIYKYAKLPQTGVSLRQMVQFGPRPNQGTLFHASYFAVHELPIRLSHRVRELENLPHGLSEEPSIKLVRDWYAISFEELMAVKEPNLTEEMKEILLKDRKDEFGWEKSPADHERRRAAKRRKEFIFEDDGIVIDRRNKDSALDKDDSASIIEVEDTKSHEETEFEKPIGLKTYFQRCPNDIVYPKEVIDYNINVTKVLTKIKRRHDATVSTLATGVQNWKQKQNHKYLNSPINQFLDRFYMSRIGIRMLIGQTIALNQQAQRNMQNDDYVGIICLHCNIKEIIEDAVDAARFACEEHFNTMEVPKVQLYCPSDLTFRYVPGHLVHMLFETLKNSLRATIEHHSKLNPGVPLDDIEYPPIKIIVAKGDEDITIKISDEGGGIPRSAIPLVWTYFYTSAEKQIKEDDMLDVTNNYRAPFMGLGVGLPHSRLYARYFSGDLKLISMEGYGTDSEKYFGKMILCARELSSRRLPLRFGLCYLQKREQSRLPAISKFENDLFNTFTKEHHHGMAQSCDDQQLFADSQFQMLKISEHGSLIQHEENTHDYLFNDQCMFDEEELNLRNTFQEKDTEINFIRNMPKYSRSKNPNETKPFRLIKLSNDAKIESSSIDDFFCRVIGNEAAVKDYHYQMPVFPTELTNYSLKHYLRQVNVIDLNYDIHGFEKLIFFQILKICRNISRFDGILDIVVLHELIKFLGKYGMIRSIFKVIDAFEHLGIHPNKDTLHLLIYKLNDIKGLRWRKDLLNFYVLLGTRKWGVSPDKTTEVLSAIQSPPGRPRLAITKRLINEGIDDSYFQNTMFHDYVTIEIESRELPYFERFYHFLLKRKAITDTHENKIILFNMYIEKLASCCKLRHAIEEMKTHEELTTPDTYTALIDQTIKNNVWTGFALLNHLMSQGNVRVSDIGMAIIGNYRTIQENFTKQAQNVNRLHQHNMPQIYNIVIKMLLAKCGYQISGNNKFLLPVERDLPLDEETVISDFQKIQFWNLDTEYSHYNGLLTSRCGGSWIFKLWDVMDIECALRAYRLRLLFHRVEMLRQSVRFFGSTRAVLQSSCKEGTPINLNIYKAGKPIVAKKDEEYPDWLWGLLDNDLQMENLKKEDWFRYNRKLIKRQNVQRIKMNNFMNNMK

Target ORF information:

RefSeq Version	XM_020688667.1
Organism	Pichia kudriavzevii(Candida krusei)
Definition	Pichia kudriavzevii [Pyruvate dehydrogenase (acetyl-transferring)] kinase, mitochondrial (BOH78_2869), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020688667.1

ORF Insert Sequence:

ATGACATTGC AAGGAAGTGG CAACGAATAT TACAGTGGGG AGACGTCTAG AGGTCCATCT 
TCCAGGTGGA GCGGTGACCA CAAAGGGGGG TTGCCGGGCA CAGACGCGGA TACGGACATG 
GACACTGCGA TGGATACCGG TCTGGGGGGT TTGGGCGACA GTCTGGGAAG CAGTAGATAC 
GACGACTTAA ATAGAAGTAG AGATAGAAGT AGAGATAGAA GCAGAAGCAG AAGCAGAAGC 
GCCAGTGTTG ATAGAAGTCT CCATGAGGGA AGAAGTATGG GATACGGAGG CGAGCGGTCA 
TACCGATCCA GAGACGAAAC TCCGAGGGCT ACATTGTACG TTAGAGGCTT CGTTACGGGG 
GTTAGGGCCA AGACGCTTGC CGATGTCTTT GAGAGGTACG GTCCAGTTGC ACAGGTGGAT 
GTGTTACCGC CACGAAGGTC AGACGGTGAG CAGTATGCGT TTGTTGCCTT TAGAAGTATT 
TCTGCCATGA ATGCAGTATT GGACGATATT GAGAGCGGGA GGCCGATCGA TGGCGGGGTG 
GCCATTGACC CCAAAGTTGG TCTTTTATGC GAGAGGGCCA GGAGTATGGA TACCTTTATG 
ACGTTTATCA ATGGGTCCCA CTCTTCAAAG AAGTTTGGAA CGGCTACCGA AGACCCAATC 
ATTGCCGGGA TGGCCAAATA CAACAACACC AATGATAAGA CGCCTCTGAG TAGTACCAGT 
GTCTCCATCT GTGGGCAGCT TTTCGAGACA GAGGCAGACG AAGTACTCAG TGAGTTTTTA 
AACCCACAGT TCTTAAAACA AGAAGACCAG GGCGACTATG AAACGCCCAG CGATCCCAAG 
CTAAGTACCA GCGGAACTAA GATGGAAACA AGTGGCACTC CCGACTCTGT TTGCACGCAG 
ACTACTGAAA CTATGAGGGT TTTGCCCAAT ACTCCCACAC CTAGTGCACC GTCCCCAACC 
ACTGTTGCCT TCAATGAGCA GCTCAAGATG TTTGAAGACT CAAAGGAGGA ATTGATGAAT 
ATTAAGTTTG GGCTTAATAA CGAGGCCAAA TACATTCACC ACTCCAATGT CGGGCTCCAA 
TACACAACCT TACCTGTAAC AGAACACCCT GAAGACCCTG ATGTAACTGC CACATATTTG 
CCCATGGAAT ATACGGAAGC GTTCCTAAGG GACTCTTCAG AGTTTCCATA CAAAATGTCC 
ATTGCTGACC TACCTGCACA ATCCAGAGTT GAAACCCAGA TAAAGCTCGA GCTTTCTGTC 
TCTCCTCCAC CTAAACAGTT TTTGTTGCAT CTGCCGAGGG ACACCATCAC AAAGCCAAAA 
TTCACATTGG CGGAAAATAC GCTTCCTGAA TCTATCAAAA AACACCTCCT TTATTTGGAG 
ATGTTTGTGA TTGGATCCGA AAGTATATCT TCCAAGGATG GCAGACTGAA GAGTTGCAAT 
GTTTGTAAAC GGTGTATGAG AAGAGAATTG AAACGGGCAT CTAGGAGGAA AGCCGGTTTG 
GTTGATGATT CTTCAAACTG GGATCTTAAC TTGCCCAAGA GAGCATTGAT AATAAACAGT 
AAAGAAATTG TTTCTTTCCC CTCCCCCAGT GGTAATGAGG TTGCTCGAAA GTTGGAGCTG 
CTATCTAGAA TTGTCTGTTA TTGCAGGCAC CATCAAGAGT TAGATGGCTT TAGGATTGCG 
ATTTTTTTGA AAAATGAACA AGATGAAATC ATAGGAAAAT GTCATACAAC ACCTATTTTG 
ATTATGGATA GGAAGAAATC TTCAAAATCT AGCAACTCAA ACAATACCGG TGCTCTCGTC 
TCCAACAAAG AACATCTTTC AGCTTCAAAG AACTCAATCA CCATTTCACA GGAGTCGTCA 
AGTGAATTTA GTATGGCAAC GTCCATATCT TCTACGAAAT TGAATCCTAA ACTAGTGACA 
AAACCTTTGG AAACTGATAT AATGATGGCG CGTGATAGCA GCAACAGCTC AATGGGTGTC 
CCTAAAACCA ACATTATGAG TATTTTGTCT TTAAAGGATG GGAAGAATAA CACCCATGGA 
AATAAGCCTC CAAAAACTGG AAGGGGAATG TTTTCTTCCA TCTTCTCCTT TGACCCTTCC 
AACTTATTGG GCGATGAATC TTCAAGGCAA ACTAAAAGAC AAAAGAGATT GTGGTCACAG 
TCCGATTCTG GAAGCTATCA CGATTCTATT AACCAACAAA GGTATCACTC GCAATCTGAA 
GCAAACAAAG GCAATGTACA AGATACCCCG GCTATGAATA GCGGGCATAT CATGCAAATC 
AAAAAAGAGG CCATTTCGCC TCTTAGCTCA GATGCTGTAT CACCGCATAA CCACATGCTG 
TCATCGGCAA CTAGCATATT TTCAGCTACG CCTGAAAGGA GGATATATGA GGGGCAGAAG 
ATGAACCTAA CATCGTCTTT CAACGATGAG TTAGCAATGA ATGTTGAAAA TATTGATAAT 
ATTGGTTCCA ATAGTGGTGT TTTAGACTTG AATGGTTTGG ATCTTGACGT TGACATGGAA 
ATGAATGTGA ATCTTAATCT TGGTGAACCA GTTCCAACAA TTCGAAAAAT TATACCTGCA 
CAGGGTTCCA TCAGAGGTGG TATTGAAGTC ACGCTTTTGG GTTCCAACTT TAAGCCGGGA 
TTAGAAGTGA AATTTGGTAG TAACATTTCT CTAGCTACGC AATGTTGGTC TGATTCCACT 
ATTGTTACGT ATTTACCTCC TGCACCACAA GCAGGGCCAG TACTAGTGAC TTTTGATTCT 
TCTGACATTG CTGCCAATAA TTCAGTTCCG CATCAAATAT TTACCTACAC CGATGATACT 
GATAAGCAAC TAATCGAGTT GGCACTCCAG ATCGTCGGTC TTAAGATGAA TGGAAAATTA 
GAGGATGCCA AGAATATTGC TAAAAGAATT GTTGGCAATA ATCAGATGGA CGATCCATCA 
TCAACCTCAT TTGGTCAAGG TGACCAATCC AATTCTTCAT CTACGTCCAA CCAATTGAAC 
CAACAACTAC AGTTGAATTG GATGGTGGTT GCTTCGAACA AAATTAAAGA ATTATCAACG 
TCTACGTTAA ACCATGAGGA CATTCTAATC AAATTCTTGA ATATCCTTCA GATCCCAAAT 
TACATTATAA CGAGTCCTAA CTGGGGAGTC TGCAATTCTG AGGGGCAAAC TATGCTCCAT 
TTGGCATGTT TGAAAAATTA TTCCAAGCTA ACTAATTTTC TTTTGAAAAG TGGCTCTAGA 
GTTGATTATA GGGACAAGAA TGGGTTTACG CCTGTGCACT TTGCCTTTAT TCCAGGAAAC 
AGAAAGATCA TCACGATGCT GATGAAATAC AACGCCAATG TCTCCAATAA ACTTGATAAT 
GGTATTTTGT TGAGTAGCAT TGCCGACTCT AATGTTCTTG ATTTGATTGA CGGACACGTT 
GACGTGAGTA GGAGGTCGTC AGACTCGACC TCTTACGAAG ACCTTGAAAT CGATGATACG 
TTTGATGACG AAATGGAGGC TTGTGGTATG GATGAAATTG GGTTCAATAG TGCATACAAA 
TCGAGCTCTA AAACGCATTA TACATCTTTG TATCGTAACA GGAAATCACA GTATGCAAGA 
AAGAAACGTG TGGATGATTC TGAAGCAGAT GATGAGGAAG AAGATGTTGA TCTGGACATG 
GATTTGGACG GTTCCGACGC TAGTACCGAC AAATCATCGT CAGGCACTAG CTCTATTAAA 
TCATTGGATC GAAAGAAAAA CACCAACTCT TTTGGTTTAA ACTTATGGAT TGCAATGAGG 
GAAGCAATAA CTAGTAAGGT CATTGAGTTG AGTGATGGTC AACATAAACA TGGTGGCAGG 
AAACATTCAC GTCCAATTCA TGGTACAAAT GTTGAAACGA GCCAACATCT CGACAAAACT 
AATGAAGCAA ATGATAATGA TGGTGAGGAG GGAGATGCAT TACCGACGTA CGACGATTTG 
TTTCCTAACG GTGCTTCTCT AAGAGCATTT ATCAATTTCC GGGGACACAC TGATGGTGAA 
GATGATGAAA AGCTATCCAA AACCAACAGA AGTGCAGAAC TAGAGGAAGA TGTTGGAGAT 
AGTGATGTGG ATATAATCGC AAAAAAGATG CACACATCCA TCACCTCAGA CACCAAGCTT 
ATGTTCTTTT GGTTGCCATT TATGCTTTTA CTATTATTGG TTGTTATTGG TGTGAAGATG 
AATATGATTT CTTTGAACAG TTTACAGTTG ATAAAGATTA GAGAAAGTGT CGGATCCTTT 
ATGCTTGGTA AGGATCGGTT CACCAACTTA CTAAACGAAA ACATCAACTA CGGACGTGAA 
CGGATGGAGA ATTTACTGCA CGATGTCAAT GGTGTATCAA CTCAATTGGC AATGTCTCAG 
TGGAAGATAA GCGAGGCACT TAGACAACAG ATATACAAAT ATGCAAAGCT GCCGCAGACA 
GGAGTCTCAC TTCGACAAAT GGTCCAATTT GGACCAAGAC CCAATCAAGG AACACTCTTT 
CACGCTTCAT ATTTTGCAGT ACATGAACTC CCCATTAGAC TAAGTCATCG TGTTCGGGAA 
TTGGAGAATT TACCGCATGG GTTGAGTGAA GAACCTTCGA TTAAATTAGT CAGAGACTGG 
TATGCAATCA GTTTTGAAGA ATTGATGGCT GTTAAGGAAC CCAATTTAAC TGAGGAAATG 
AAGGAGATTT TGTTGAAAGA CAGGAAAGAT GAATTTGGCT GGGAGAAATC ACCTGCTGAC 
CATGAGAGGC GACGCGCAGC GAAAAGGCGC AAAGAGTTCA TATTTGAAGA TGACGGTATT 
GTCATTGATA GAAGAAATAA GGATTCAGCG CTTGATAAGG ACGATAGTGC ATCCATTATT 
GAAGTAGAAG ATACGAAAAG CCATGAAGAA ACCGAATTCG AGAAGCCTAT TGGACTAAAA 
ACATATTTCC AAAGGTGTCC TAATGACATT GTTTATCCAA AAGAAGTTAT AGATTACAAT 
ATCAACGTCA CAAAGGTATT AACGAAAATC AAGAGAAGAC ATGACGCCAC TGTTTCTACA 
TTGGCTACCG GTGTCCAAAA TTGGAAACAA AAGCAAAATC ATAAATACTT GAACAGTCCG 
ATAAATCAGT TTTTAGATAG ATTTTATATG AGCAGAATCG GTATTAGAAT GTTAATTGGC 
CAAACGATAG CACTTAATCA ACAAGCACAA AGAAACATGC AAAATGACGA CTATGTCGGT 
ATAATTTGTC TGCACTGTAA CATTAAAGAA ATTATTGAAG ATGCTGTTGA CGCAGCACGC 
TTTGCATGCG AAGAACATTT TAATACAATG GAAGTTCCCA AAGTCCAACT ATATTGTCCT 
TCAGATTTGA CCTTCAGATA TGTTCCTGGA CATTTGGTTC ACATGCTATT CGAGACTCTC 
AAGAATAGTC TAAGAGCAAC AATTGAACAT CATTCCAAAT TGAATCCAGG CGTACCTCTT 
GATGATATCG AGTATCCACC AATCAAAATC ATTGTTGCCA AGGGGGATGA AGATATAACC 
ATCAAGATTT CCGATGAAGG TGGAGGAATT CCAAGATCTG CAATCCCACT AGTTTGGACT 
TATTTCTATA CCTCGGCAGA GAAACAAATA AAAGAAGACG ATATGCTCGA CGTTACCAAT 
AACTATAGAG CTCCATTTAT GGGGCTAGGC GTTGGGTTAC CACATTCTCG TTTGTATGCT 
AGATACTTCT CTGGTGACTT AAAATTGATT AGTATGGAGG GTTACGGAAC AGACTCAGAG 
AAGTATTTTG GTAAAATGAT ACTCTGTGCA AGGGAGCTAT CCTCCAGACG ACTTCCATTG 
CGGTTTGGGC TATGTTATTT GCAGAAGAGA GAACAATCGA GACTTCCTGC CATATCGAAA 
TTCGAAAATG ATTTGTTCAA TACATTCACA AAAGAACATC ACCATGGAAT GGCGCAGTCT 
TGTGACGACC AGCAATTATT TGCAGATTCA CAATTTCAGA TGTTGAAAAT ATCCGAACAT 
GGATCATTAA TTCAGCATGA AGAAAATACA CATGATTATC TGTTCAATGA TCAATGTATG 
TTTGACGAGG AGGAATTAAA TTTACGAAAT ACGTTTCAAG AAAAGGATAC AGAGATAAAT 
TTCATTAGAA ATATGCCAAA ATATTCACGG TCGAAAAACC CAAACGAAAC CAAACCCTTT 
AGGTTAATTA AACTGTCGAA TGATGCTAAA ATAGAAAGCA GTTCAATTGA CGATTTTTTT 
TGTCGAGTTA TAGGAAATGA AGCCGCTGTA AAGGATTACC ATTACCAGAT GCCTGTTTTC 
CCCACTGAAT TGACCAACTA TTCTTTAAAA CATTACTTGC GACAAGTTAA TGTCATCGAT 
TTAAATTATG ATATACATGG GTTTGAGAAA CTGATCTTTT TTCAGATACT GAAAATCTGT 
CGAAATATCA GCCGGTTTGA TGGAATACTG GATATTGTGG TACTTCATGA ACTGATTAAA 
TTTCTAGGTA AATATGGCAT GATACGATCT ATATTCAAAG TCATTGATGC TTTTGAACAT 
TTGGGGATTC ATCCAAATAA AGACACTCTT CATTTGCTGA TATACAAACT AAATGACATT 
AAAGGTCTAC GATGGAGAAA GGATCTTCTC AATTTCTATG TTCTTCTTGG TACCCGAAAA 
TGGGGGGTAT CGCCGGACAA AACGACAGAG GTACTTTCGG CAATACAAAG CCCTCCTGGA 
AGACCCCGGC TGGCTATTAC CAAACGACTA ATCAATGAAG GAATTGATGA CTCGTATTTC 
CAAAACACTA TGTTTCACGA TTATGTCACT ATAGAAATTG AAAGCCGTGA ACTCCCGTAT 
TTCGAGAGAT TCTACCATTT TTTACTTAAA AGAAAAGCAA TCACAGATAC CCATGAAAAC 
AAAATAATAT TATTCAACAT GTACATAGAA AAACTAGCAT CTTGCTGCAA ATTGAGGCAT 
GCAATTGAGG AAATGAAGAC GCATGAAGAG CTAACCACGC CGGATACATA CACTGCATTA 
ATTGACCAAA CAATCAAGAA TAATGTTTGG ACAGGGTTTG CCCTTCTCAA TCATTTAATG 
AGCCAAGGAA ACGTCCGTGT TTCCGATATA GGCATGGCGA TAATCGGCAA CTACCGTACT 
ATTCAGGAAA ACTTTACTAA GCAAGCGCAA AATGTTAATA GACTACACCA GCACAACATG 
CCACAAATAT ACAACATTGT CATTAAGATG TTACTGGCTA AATGTGGATA TCAAATAAGC 
GGCAACAACA AGTTCCTTCT ACCGGTGGAG AGAGACCTAC CACTAGATGA GGAGACGGTT 
ATATCTGACT TCCAAAAGAT TCAGTTTTGG AACTTAGATA CGGAATACTC ACACTACAAT 
GGCCTCTTGA CGTCTCGCTG TGGCGGATCA TGGATATTCA AACTCTGGGA TGTTATGGAT 
ATCGAGTGTG CACTTAGAGC CTACAGGTTG CGTTTATTGT TCCATCGAGT AGAGATGTTG 
AGACAAAGTG TGAGATTCTT TGGTAGCACC CGTGCAGTAC TTCAGTCTTC CTGTAAAGAA 
GGCACCCCAA TCAACTTGAA TATCTACAAG GCGGGCAAGC CAATTGTTGC AAAGAAGGAC 
GAGGAATACC CCGACTGGCT GTGGGGTTTA CTGGACAATG ACCTGCAAAT GGAAAACTTG 
AAGAAGGAAG ACTGGTTCAG ATATAATAGG AAACTCATCA AGAGACAAAA TGTCCAACGT 
ATAAAGATGA ACAACTTCAT GAACAACATG AAATAA