PBRM1 gene cDNA ORF clone, Pongo abelii(Sumatran orangutan)

mRNA	Protein	Name
XM_024244386.1	XP_024100154.1	protein polybromo-1 isoform X1
XM_024244390.1	XP_024100158.1	protein polybromo-1 isoform X3
XM_024244396.1	XP_024100164.1	protein polybromo-1 isoform X8
XM_024244400.1	XP_024100168.1	protein polybromo-1 isoform X12
XM_024244403.1	XP_024100171.1	protein polybromo-1 isoform X13
XM_009238921.1	XP_009237196.1	protein polybromo-1
XM_024244388.1	XP_024100156.1	protein polybromo-1 isoform X1
XM_009238922.2	XP_009237197.2	protein polybromo-1 isoform X1
XM_009238923.2	XP_009237198.2	protein polybromo-1 isoform X1
XM_024244397.1	XP_024100165.1	protein polybromo-1 isoform X9
XM_009238923.1	XP_009237198.1	protein polybromo-1
XM_024244394.1	XP_024100162.1	protein polybromo-1 isoform X7
XM_024244398.1	XP_024100166.1	protein polybromo-1 isoform X10
XM_024244404.1	XP_024100172.1	protein polybromo-1 isoform X14
XM_009238922.1	XP_009237197.1	protein polybromo-1
XM_009238924.1	XP_009237199.1	protein polybromo-1
XM_009238921.2	XP_009237196.2	protein polybromo-1 isoform X1
XM_024244391.1	XP_024100159.1	protein polybromo-1 isoform X4
XM_024244395.1	XP_024100163.1	protein polybromo-1 isoform X8
XM_024244399.1	XP_024100167.1	protein polybromo-1 isoform X11
XM_024244401.1	XP_024100169.1	protein polybromo-1 isoform X12
XM_024244389.1	XP_024100157.1	protein polybromo-1 isoform X2
XM_024244392.1	XP_024100160.1	protein polybromo-1 isoform X5
XM_024244393.1	XP_024100161.1	protein polybromo-1 isoform X6
XM_024244402.1	XP_024100170.1	protein polybromo-1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa179447	XM_024244402.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa179447	XM_009238921.2 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa179447	XM_024244386.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa179447	XM_009238923.2 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa179447	XM_024244388.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa179447	XM_009238922.2 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa179448	XM_024244389.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa179449	XM_024244390.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa179450	XM_024244391.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSa179451	XM_024244392.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179452	XM_024244393.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179453	XM_024244394.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179454	XM_024244396.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179454	XM_024244395.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179455	XM_024244397.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179456	XM_024244398.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179457	XM_024244399.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179458	XM_024244401.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179458	XM_024244400.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179459	XM_024244403.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa179460	XM_024244404.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OSa34489	XM_009238921.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSa34489	XM_009238922.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSa34489	XM_009238923.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OSa34489	XM_009238924.1 Latest version!	Pongo abelii polybromo 1 (PBRM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

PBRM1 ( XM_024244386.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100154.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa179447
Clone ID Related Accession (Same CDS sequence)	XM_024244386.1 , XM_024244388.1 , XM_009238922.2 , XM_009238923.2 , XM_009238921.2 , XM_024244402.1
Accession Version	XM_024244386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100154.1
CDS	245..5359
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244386.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244386.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244388.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100156.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X2, mRNA.

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CloneID	OSa179447
Clone ID Related Accession (Same CDS sequence)	XM_024244386.1 , XM_024244388.1 , XM_009238922.2 , XM_009238923.2 , XM_009238921.2 , XM_024244402.1
Accession Version	XM_024244388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100156.1
CDS	327..5441
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244388.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244388.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_009238922.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009237197.2 Pongo abelii polybromo 1 (PBRM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSa179447
Clone ID Related Accession (Same CDS sequence)	XM_024244386.1 , XM_024244388.1 , XM_009238922.2 , XM_009238923.2 , XM_009238921.2 , XM_024244402.1
Accession Version	XM_009238922.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009238922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009237197.2
CDS	348..5462
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_009238922.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009238922.2

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_009238923.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009237198.2 Pongo abelii polybromo 1 (PBRM1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSa179447
Clone ID Related Accession (Same CDS sequence)	XM_024244386.1 , XM_024244388.1 , XM_009238922.2 , XM_009238923.2 , XM_009238921.2 , XM_024244402.1
Accession Version	XM_009238923.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009238923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009237198.2
CDS	109..5223
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_009238923.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009238923.2

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_009238921.2 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009237196.2 Pongo abelii polybromo 1 (PBRM1), transcript variant X3, mRNA.

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CloneID	OSa179447
Clone ID Related Accession (Same CDS sequence)	XM_024244386.1 , XM_024244388.1 , XM_009238922.2 , XM_009238923.2 , XM_009238921.2 , XM_024244402.1
Accession Version	XM_009238921.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_009238921.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009237196.2
CDS	245..5359
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_009238921.2
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009238921.2

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244402.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100170.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X19, mRNA.

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CloneID	OSa179447
Clone ID Related Accession (Same CDS sequence)	XM_024244386.1 , XM_024244388.1 , XM_009238922.2 , XM_009238923.2 , XM_009238921.2 , XM_024244402.1
Accession Version	XM_024244402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100170.1
CDS	210..5324
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244402.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244402.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244389.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100157.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X6, mRNA.

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CloneID	OSa179448
Clone ID Related Accession (Same CDS sequence)	XM_024244389.1
Accession Version	XM_024244389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGCTGAAA AGAGTGAAGA TTCCTCTGGT GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGTAGCTT TAAAAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAACCTGCAG AGGCCAACCT ACAACCACAT ATTGTCTGTA TTGAAAGACT GTGGGAGGAT 
TCAGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTACC GACCAAATGA AACATTCCAC 
CTGGCTACAC GAAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAACAAAGTT 
CCAGTTAGTA AAATTCTAGG CAAGTGTGTG GTCATGTTTG TCAAGGAATA CTTTAAGTTA 
TGCCCAGAAA ACTTCCGAGA TGAGGATGTT TTTGTCTGTG AATCACGGTA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCA TCAGCTCAGT TAGGTTTGTC 
CCTCGGGATG TGCCTCTGCC TGTGGTTCGC GTGGCCTCTG TATTTGCAAA TGCAGATAAA 
GGTGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAGCTGAAGA CAATTTTAAC 
TTGGAAAAGG AAAAAGAAGA TGTCCCTGTG GAAATGTCCA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCATTA CAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GCCTGGTGCG TCCTCGTGTG GGCAGAATTG AAAAAGTATG GGTTCGAGAT 
GGAGCTGCAT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAAACGGA GCATGAGCCC 
ACAAAAATGT TCTACAAAAA AGAAGTATTT CTGAGTAATC TGGAAGAAAC CTGCCCCATG 
ACATGTATTC TCGGAAAGTG TGCTGTGTTG TCATTCAAAG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATAC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAATGA AAGTGACAAG 
CAGATGAAGA AATTCAAAGG ATTGAAGAGG TTTTCACTCT CTGCTAAAGT GGTAGATGAT 
GAAATTTACT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCATCACC TTTGTTGGAA 
AAGAAGATCC AGTTGCTGGA AGCTAAATTT GCCGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAAGA TAGTGAGGTC ATTGAACCTC CTTCTCTACC TCAGCTTCAG 
ACCCCCCTGG CCAGTGAGCT GGACCTCATG CCCTACACAC CCCCACAGTC TACCCCAAAG 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AAATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGGGCTGTG ATTAAGGCCC AACACCCAGA TTACTCTTTC 
GGGGAGCTCA GCCGCCTGGT GGGGACAGAA TGGAGAAATC TTGAGACAGC CAAGAAAGCG 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCTGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCA TATGGACAAC AGGTGGGAGT TTTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCC GGCCCACATC CACCTGGACC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTAGCT CCCCCACCAA AGACCCAGCG GCTTCTTCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCCG AGTCCAACAG CATTAGCAAG 
TGGGATCAGA CACTGGCAGC TCGAAGACGT GACGTCCATT TGTCAAAAGA ACAGGAGAGC 
CGCCTACCCT CTCACTGGCT GAAAAGCAAA GGGGCCCACA CCACCATGGC AGATGCCCTC 
TGGCGCCTTC GAGATTTGAT GCTGCGGGAC ACTCTCAACA TTCGCCAAGC ATACAACCTA 
GAAAACATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100157.1
CDS	327..5438
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKRAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244389.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244389.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGCTGAAA AGAGTGAAGA TTCCTCTGGT GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGTAGCTT TAAAAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAACCTGCAG AGGCCAACCT ACAACCACAT ATTGTCTGTA TTGAAAGACT GTGGGAGGAT 
TCAGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTACC GACCAAATGA AACATTCCAC 
CTGGCTACAC GAAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAACAAAGTT 
CCAGTTAGTA AAATTCTAGG CAAGTGTGTG GTCATGTTTG TCAAGGAATA CTTTAAGTTA 
TGCCCAGAAA ACTTCCGAGA TGAGGATGTT TTTGTCTGTG AATCACGGTA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCA TCAGCTCAGT TAGGTTTGTC 
CCTCGGGATG TGCCTCTGCC TGTGGTTCGC GTGGCCTCTG TATTTGCAAA TGCAGATAAA 
GGTGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAGCTGAAGA CAATTTTAAC 
TTGGAAAAGG AAAAAGAAGA TGTCCCTGTG GAAATGTCCA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCATTA CAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GCCTGGTGCG TCCTCGTGTG GGCAGAATTG AAAAAGTATG GGTTCGAGAT 
GGAGCTGCAT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAAACGGA GCATGAGCCC 
ACAAAAATGT TCTACAAAAA AGAAGTATTT CTGAGTAATC TGGAAGAAAC CTGCCCCATG 
ACATGTATTC TCGGAAAGTG TGCTGTGTTG TCATTCAAAG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATAC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAATGA AAGTGACAAG 
CAGATGAAGA AATTCAAAGG ATTGAAGAGG TTTTCACTCT CTGCTAAAGT GGTAGATGAT 
GAAATTTACT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCATCACC TTTGTTGGAA 
AAGAAGATCC AGTTGCTGGA AGCTAAATTT GCCGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAAGA TAGTGAGGTC ATTGAACCTC CTTCTCTACC TCAGCTTCAG 
ACCCCCCTGG CCAGTGAGCT GGACCTCATG CCCTACACAC CCCCACAGTC TACCCCAAAG 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AAATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGGGCTGTG ATTAAGGCCC AACACCCAGA TTACTCTTTC 
GGGGAGCTCA GCCGCCTGGT GGGGACAGAA TGGAGAAATC TTGAGACAGC CAAGAAAGCG 
GAATATGAAG AGCGGGCAGC TAAAGTTGCT GAGCAGCAGG AGAGAGAGCG AGCAGCACAG 
CAACAGCAGC CGAGTGCTTC TCCCCGAGCA GGCACCCCTG TGGGGGCTCT CATGGGGGTG 
GTGCCACCAC CAACACCAAT GGGGATGCTC AATCAGCAGT TGACACCTGT TGCAGGCATG 
ATGGGTGGCT ATCCGCCAGG CCTTCCACCT TTGCAGGGCC CAGTTGATGG CCTTGTTAGC 
ATGGGCAGCA TGCAGCCACT TCACCCTGGG GGGCCTCCAC CCCACCATCT TCCGCCAGGT 
GTGCCTGGCC TCCCGGGCAT CCCACCACCG GGTGTGATGA ACCAAGGAGT GGCCCCTATG 
GTAGGGACTC CAGCACCAGG TGGAAGTCCA TATGGACAAC AGGTGGGAGT TTTGGGGCCT 
CCAGGGCAGC AGGCACCACC TCCATATCCC GGCCCACATC CACCTGGACC CCCTGTCATA 
CAGCAGCCAA CAACACCCAT GTTTGTAGCT CCCCCACCAA AGACCCAGCG GCTTCTTCAC 
TCAGAGGCCT ACCTGAAATA CATTGAAGGA CTCAGTGCCG AGTCCAACAG CATTAGCAAG 
TGGGATCAGA CACTGGCAGC TCGAAGACGT GACGTCCATT TGTCAAAAGA ACAGGAGAGC 
CGCCTACCCT CTCACTGGCT GAAAAGCAAA GGGGCCCACA CCACCATGGC AGATGCCCTC 
TGGCGCCTTC GAGATTTGAT GCTGCGGGAC ACTCTCAACA TTCGCCAAGC ATACAACCTA 
GAAAACATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244390.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100158.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X7, mRNA.

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CloneID	OSa179449
Clone ID Related Accession (Same CDS sequence)	XM_024244390.1
Accession Version	XM_024244390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCATA TGGACAACAG 
GTGGGAGTTT TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCCGG CCCACATCCA 
CCTGGACCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTAGCTCC CCCACCAAAG 
ACCCAGCGGC TTCTTCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCCGAG 
TCCAACAGCA TTAGCAAGTG GGATCAGACA CTGGCAGCTC GAAGACGTGA CGTCCATTTG 
TCAAAAGAAC AGGAGAGCCG CCTACCCTCT CACTGGCTGA AAAGCAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTTCGA GATTTGATGC TGCGGGACAC TCTCAACATT 
CGCCAAGCAT ACAACCTAGA AAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100158.1
CDS	327..5396
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244390.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244390.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGAG CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG 
AGAGAGCGAG CAGCACAGCA ACAGCAGCCG AGTGCTTCTC CCCGAGCAGG CACCCCTGTG 
GGGGCTCTCA TGGGGGTGGT GCCACCACCA ACACCAATGG GGATGCTCAA TCAGCAGTTG 
ACACCTGTTG CAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCATA TGGACAACAG 
GTGGGAGTTT TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCCGG CCCACATCCA 
CCTGGACCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTAGCTCC CCCACCAAAG 
ACCCAGCGGC TTCTTCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCCGAG 
TCCAACAGCA TTAGCAAGTG GGATCAGACA CTGGCAGCTC GAAGACGTGA CGTCCATTTG 
TCAAAAGAAC AGGAGAGCCG CCTACCCTCT CACTGGCTGA AAAGCAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTTCGA GATTTGATGC TGCGGGACAC TCTCAACATT 
CGCCAAGCAT ACAACCTAGA AAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244391.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100159.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X8, mRNA.

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CloneID	OSa179450
Clone ID Related Accession (Same CDS sequence)	XM_024244391.1
Accession Version	XM_024244391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG AAAAAGAAGT TTTTAAGAGT GACTATTACA ACAAAGTTCC AGTTAGTAAA 
ATTCTAGGCA AGTGTGTGGT CATGTTTGTC AAGGAATACT TTAAGTTATG CCCAGAAAAC 
TTCCGAGATG AGGATGTTTT TGTCTGTGAA TCACGGTATT CTGCCAAAAC CAAATCTTTT 
AAGAAAATTA AACTGTGGAC CATGCCCATC AGCTCAGTTA GGTTTGTCCC TCGGGATGTG 
CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA TTTGCAAATG CAGATAAAGG TGATGATGAG 
AAGAATACAG ACAACTCAGA GGACAGTCGA GCTGAAGACA ATTTTAACTT GGAAAAGGAA 
AAAGAAGATG TCCCTGTGGA AATGTCCAAT GGTGAACCAG GTTGCCACTA CTTTGAGCAG 
CTCCATTACA ATGACATGTG GCTGAAGGTT GGCGACTGTG TCTTCATCAA GTCCCATGGC 
CTGGTGCGTC CTCGTGTGGG CAGAATTGAA AAAGTATGGG TTCGAGATGG AGCTGCATAT 
TTTTATGGCC CCATCTTCAT TCACCCAGAA GAAACGGAGC ATGAGCCCAC AAAAATGTTC 
TACAAAAAAG AAGTATTTCT GAGTAATCTG GAAGAAACCT GCCCCATGAC ATGTATTCTC 
GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC TTCCTCTCCT GCAGGCCAAC TGAAATACCA 
GAAAATGACA TTCTGCTTTG TGAGAGCCGC TACAATGAAA GTGACAAGCA GATGAAGAAA 
TTCAAAGGAT TGAAGAGGTT TTCACTCTCT GCTAAAGTGG TAGATGATGA AATTTACTAC 
TTCAGAAAAC CAATTGTTCC TCAGAAGGAG CCATCACCTT TGTTGGAAAA GAAGATCCAG 
TTGCTGGAAG CTAAATTTGC CGAGTTAGAA GGTGGAGATG ATGATATTGA AGAGATGGGA 
GAAGAAGATA GTGAGGTCAT TGAACCTCCT TCTCTACCTC AGCTTCAGAC CCCCCTGGCC 
AGTGAGCTGG ACCTCATGCC CTACACACCC CCACAGTCTA CCCCAAAGTC TGCCAAAGGC 
AGTGCAAAGA AGGAAGGCTC CAAACGGAAA ATCAACATGA GTGGCTACAT CCTGTTCAGC 
AGTGAGATGA GGGCTGTGAT TAAGGCCCAA CACCCAGATT ACTCTTTCGG GGAGCTCAGC 
CGCCTGGTGG GGACAGAATG GAGAAATCTT GAGACAGCCA AGAAAGCGGA ATATGAAGAG 
CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG AGAGAGCGAG CAGCACAGCA ACAGCAGCCG 
AGTGCTTCTC CCCGAGCAGG CACCCCTGTG GGGGCTCTCA TGGGGGTGGT GCCACCACCA 
ACACCAATGG GGATGCTCAA TCAGCAGTTG ACACCTGTTG CAGGCATGAT GGGTGGCTAT 
CCGCCAGGCC TTCCACCTTT GCAGGGCCCA GTTGATGGCC TTGTTAGCAT GGGCAGCATG 
CAGCCACTTC ACCCTGGGGG GCCTCCACCC CACCATCTTC CGCCAGGTGT GCCTGGCCTC 
CCGGGCATCC CACCACCGGG TGTGATGAAC CAAGGAGTGG CCCCTATGGT AGGGACTCCA 
GCACCAGGTG GAAGTCCATA TGGACAACAG GTGGGAGTTT TGGGGCCTCC AGGGCAGCAG 
GCACCACCTC CATATCCCGG CCCACATCCA CCTGGACCCC CTGTCATACA GCAGCCAACA 
ACACCCATGT TTGTAGCTCC CCCACCAAAG ACCCAGCGGC TTCTTCACTC AGAGGCCTAC 
CTGAAATACA TTGAAGGACT CAGTGCCGAG TCCAACAGCA TTAGCAAGTG GGATCAGACA 
CTGGCAGCTC GAAGACGTGA CGTCCATTTG TCAAAAGAAC AGGAGAGCCG CCTACCCTCT 
CACTGGCTGA AAAGCAAAGG GGCCCACACC ACCATGGCAG ATGCCCTCTG GCGCCTTCGA 
GATTTGATGC TGCGGGACAC TCTCAACATT CGCCAAGCAT ACAACCTAGA AAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100159.1
CDS	327..5366
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244391.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244391.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG AAAAAGAAGT TTTTAAGAGT GACTATTACA ACAAAGTTCC AGTTAGTAAA 
ATTCTAGGCA AGTGTGTGGT CATGTTTGTC AAGGAATACT TTAAGTTATG CCCAGAAAAC 
TTCCGAGATG AGGATGTTTT TGTCTGTGAA TCACGGTATT CTGCCAAAAC CAAATCTTTT 
AAGAAAATTA AACTGTGGAC CATGCCCATC AGCTCAGTTA GGTTTGTCCC TCGGGATGTG 
CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA TTTGCAAATG CAGATAAAGG TGATGATGAG 
AAGAATACAG ACAACTCAGA GGACAGTCGA GCTGAAGACA ATTTTAACTT GGAAAAGGAA 
AAAGAAGATG TCCCTGTGGA AATGTCCAAT GGTGAACCAG GTTGCCACTA CTTTGAGCAG 
CTCCATTACA ATGACATGTG GCTGAAGGTT GGCGACTGTG TCTTCATCAA GTCCCATGGC 
CTGGTGCGTC CTCGTGTGGG CAGAATTGAA AAAGTATGGG TTCGAGATGG AGCTGCATAT 
TTTTATGGCC CCATCTTCAT TCACCCAGAA GAAACGGAGC ATGAGCCCAC AAAAATGTTC 
TACAAAAAAG AAGTATTTCT GAGTAATCTG GAAGAAACCT GCCCCATGAC ATGTATTCTC 
GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC TTCCTCTCCT GCAGGCCAAC TGAAATACCA 
GAAAATGACA TTCTGCTTTG TGAGAGCCGC TACAATGAAA GTGACAAGCA GATGAAGAAA 
TTCAAAGGAT TGAAGAGGTT TTCACTCTCT GCTAAAGTGG TAGATGATGA AATTTACTAC 
TTCAGAAAAC CAATTGTTCC TCAGAAGGAG CCATCACCTT TGTTGGAAAA GAAGATCCAG 
TTGCTGGAAG CTAAATTTGC CGAGTTAGAA GGTGGAGATG ATGATATTGA AGAGATGGGA 
GAAGAAGATA GTGAGGTCAT TGAACCTCCT TCTCTACCTC AGCTTCAGAC CCCCCTGGCC 
AGTGAGCTGG ACCTCATGCC CTACACACCC CCACAGTCTA CCCCAAAGTC TGCCAAAGGC 
AGTGCAAAGA AGGAAGGCTC CAAACGGAAA ATCAACATGA GTGGCTACAT CCTGTTCAGC 
AGTGAGATGA GGGCTGTGAT TAAGGCCCAA CACCCAGATT ACTCTTTCGG GGAGCTCAGC 
CGCCTGGTGG GGACAGAATG GAGAAATCTT GAGACAGCCA AGAAAGCGGA ATATGAAGAG 
CGGGCAGCTA AAGTTGCTGA GCAGCAGGAG AGAGAGCGAG CAGCACAGCA ACAGCAGCCG 
AGTGCTTCTC CCCGAGCAGG CACCCCTGTG GGGGCTCTCA TGGGGGTGGT GCCACCACCA 
ACACCAATGG GGATGCTCAA TCAGCAGTTG ACACCTGTTG CAGGCATGAT GGGTGGCTAT 
CCGCCAGGCC TTCCACCTTT GCAGGGCCCA GTTGATGGCC TTGTTAGCAT GGGCAGCATG 
CAGCCACTTC ACCCTGGGGG GCCTCCACCC CACCATCTTC CGCCAGGTGT GCCTGGCCTC 
CCGGGCATCC CACCACCGGG TGTGATGAAC CAAGGAGTGG CCCCTATGGT AGGGACTCCA 
GCACCAGGTG GAAGTCCATA TGGACAACAG GTGGGAGTTT TGGGGCCTCC AGGGCAGCAG 
GCACCACCTC CATATCCCGG CCCACATCCA CCTGGACCCC CTGTCATACA GCAGCCAACA 
ACACCCATGT TTGTAGCTCC CCCACCAAAG ACCCAGCGGC TTCTTCACTC AGAGGCCTAC 
CTGAAATACA TTGAAGGACT CAGTGCCGAG TCCAACAGCA TTAGCAAGTG GGATCAGACA 
CTGGCAGCTC GAAGACGTGA CGTCCATTTG TCAAAAGAAC AGGAGAGCCG CCTACCCTCT 
CACTGGCTGA AAAGCAAAGG GGCCCACACC ACCATGGCAG ATGCCCTCTG GCGCCTTCGA 
GATTTGATGC TGCGGGACAC TCTCAACATT CGCCAAGCAT ACAACCTAGA AAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244392.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100160.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OSa179451
Clone ID Related Accession (Same CDS sequence)	XM_024244392.1
Accession Version	XM_024244392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100160.1
CDS	327..5321
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244392.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244392.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244393.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100161.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X10, mRNA.

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CloneID	OSa179452
Clone ID Related Accession (Same CDS sequence)	XM_024244393.1
Accession Version	XM_024244393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGT 
GTGATGAACC AAGGAGTGGC CCCTATGGTA GGGACTCCAG CACCAGGTGG AAGTCCATAT 
GGACAACAGG TGGGAGTTTT GGGGCCTCCA GGGCAGCAGG CACCACCTCC ATATCCCGGC 
CCACATCCAC CTGGACCCCC TGTCATACAG CAGCCAACAA CACCCATGTT TGTAGCTCCC 
CCACCAAAGA CCCAGCGGCT TCTTCACTCA GAGGCCTACC TGAAATACAT TGAAGGACTC 
AGTGCCGAGT CCAACAGCAT TAGCAAGTGG GATCAGACAC TGGCAGCTCG AAGACGTGAC 
GTCCATTTGT CAAAAGAACA GGAGAGCCGC CTACCCTCTC ACTGGCTGAA AAGCAAAGGG 
GCCCACACCA CCATGGCAGA TGCCCTCTGG CGCCTTCGAG ATTTGATGCT GCGGGACACT 
CTCAACATTC GCCAAGCATA CAACCTAGAA AACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100161.1
CDS	327..5285
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244393.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244393.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGT 
GTGATGAACC AAGGAGTGGC CCCTATGGTA GGGACTCCAG CACCAGGTGG AAGTCCATAT 
GGACAACAGG TGGGAGTTTT GGGGCCTCCA GGGCAGCAGG CACCACCTCC ATATCCCGGC 
CCACATCCAC CTGGACCCCC TGTCATACAG CAGCCAACAA CACCCATGTT TGTAGCTCCC 
CCACCAAAGA CCCAGCGGCT TCTTCACTCA GAGGCCTACC TGAAATACAT TGAAGGACTC 
AGTGCCGAGT CCAACAGCAT TAGCAAGTGG GATCAGACAC TGGCAGCTCG AAGACGTGAC 
GTCCATTTGT CAAAAGAACA GGAGAGCCGC CTACCCTCTC ACTGGCTGAA AAGCAAAGGG 
GCCCACACCA CCATGGCAGA TGCCCTCTGG CGCCTTCGAG ATTTGATGCT GCGGGACACT 
CTCAACATTC GCCAAGCATA CAACCTAGAA AACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244394.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100162.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X11, mRNA.

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CloneID	OSa179453
Clone ID Related Accession (Same CDS sequence)	XM_024244394.1
Accession Version	XM_024244394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCATA TGGACAACAG 
GTGGGAGTTT TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCCGG CCCACATCCA 
CCTGGACCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTAGCTCC CCCACCAAAG 
ACCCAGCGGC TTCTTCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCCGAG 
TCCAACAGCA TTAGCAAGTG GGATCAGACA CTGGCAGCTC GAAGACGTGA CGTCCATTTG 
TCAAAAGAAC AGGAGAGCCG CCTACCCTCT CACTGGCTGA AAAGCAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTTCGA GATTTGATGC TGCGGGACAC TCTCAACATT 
CGCCAAGCAT ACAACCTAGA AAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100162.1
CDS	327..5276
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244394.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244394.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCATA TGGACAACAG 
GTGGGAGTTT TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCCGG CCCACATCCA 
CCTGGACCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTAGCTCC CCCACCAAAG 
ACCCAGCGGC TTCTTCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCCGAG 
TCCAACAGCA TTAGCAAGTG GGATCAGACA CTGGCAGCTC GAAGACGTGA CGTCCATTTG 
TCAAAAGAAC AGGAGAGCCG CCTACCCTCT CACTGGCTGA AAAGCAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTTCGA GATTTGATGC TGCGGGACAC TCTCAACATT 
CGCCAAGCAT ACAACCTAGA AAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244396.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100164.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X13, mRNA.

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CloneID	OSa179454
Clone ID Related Accession (Same CDS sequence)	XM_024244396.1 , XM_024244395.1
Accession Version	XM_024244396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCATATGGAC AACAGGTGGG AGTTTTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCCGGCCCAC ATCCACCTGG ACCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTA 
GCTCCCCCAC CAAAGACCCA GCGGCTTCTT CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CCGAGTCCAA CAGCATTAGC AAGTGGGATC AGACACTGGC AGCTCGAAGA 
CGTGACGTCC ATTTGTCAAA AGAACAGGAG AGCCGCCTAC CCTCTCACTG GCTGAAAAGC 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTCTGGCGCC TTCGAGATTT GATGCTGCGG 
GACACTCTCA ACATTCGCCA AGCATACAAC CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100164.1
CDS	112..5016
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244396.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244396.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCATATGGAC AACAGGTGGG AGTTTTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCCGGCCCAC ATCCACCTGG ACCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTA 
GCTCCCCCAC CAAAGACCCA GCGGCTTCTT CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CCGAGTCCAA CAGCATTAGC AAGTGGGATC AGACACTGGC AGCTCGAAGA 
CGTGACGTCC ATTTGTCAAA AGAACAGGAG AGCCGCCTAC CCTCTCACTG GCTGAAAAGC 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTCTGGCGCC TTCGAGATTT GATGCTGCGG 
GACACTCTCA ACATTCGCCA AGCATACAAC CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244395.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100163.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X12, mRNA.

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CloneID	OSa179454
Clone ID Related Accession (Same CDS sequence)	XM_024244396.1 , XM_024244395.1
Accession Version	XM_024244395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCATATGGAC AACAGGTGGG AGTTTTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCCGGCCCAC ATCCACCTGG ACCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTA 
GCTCCCCCAC CAAAGACCCA GCGGCTTCTT CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CCGAGTCCAA CAGCATTAGC AAGTGGGATC AGACACTGGC AGCTCGAAGA 
CGTGACGTCC ATTTGTCAAA AGAACAGGAG AGCCGCCTAC CCTCTCACTG GCTGAAAAGC 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTCTGGCGCC TTCGAGATTT GATGCTGCGG 
GACACTCTCA ACATTCGCCA AGCATACAAC CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100163.1
CDS	327..5231
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244395.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244395.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGGC ATGATGGGTG GCTATCCGCC AGGCCTTCCA 
CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT AGCATGGGCA GCATGCAGCC ACTTCACCCT 
GGGGGGCCTC CACCCCACCA TCTTCCGCCA GGTGTGCCTG GCCTCCCGGG CATCCCACCA 
CCGGGTGTGA TGAACCAAGG AGTGGCCCCT ATGGTAGGGA CTCCAGCACC AGGTGGAAGT 
CCATATGGAC AACAGGTGGG AGTTTTGGGG CCTCCAGGGC AGCAGGCACC ACCTCCATAT 
CCCGGCCCAC ATCCACCTGG ACCCCCTGTC ATACAGCAGC CAACAACACC CATGTTTGTA 
GCTCCCCCAC CAAAGACCCA GCGGCTTCTT CACTCAGAGG CCTACCTGAA ATACATTGAA 
GGACTCAGTG CCGAGTCCAA CAGCATTAGC AAGTGGGATC AGACACTGGC AGCTCGAAGA 
CGTGACGTCC ATTTGTCAAA AGAACAGGAG AGCCGCCTAC CCTCTCACTG GCTGAAAAGC 
AAAGGGGCCC ACACCACCAT GGCAGATGCC CTCTGGCGCC TTCGAGATTT GATGCTGCGG 
GACACTCTCA ACATTCGCCA AGCATACAAC CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244397.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100165.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OSa179455
Clone ID Related Accession (Same CDS sequence)	XM_024244397.1
Accession Version	XM_024244397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGC TGAAAAGAGT GAAGATTCCT CTGGTGCTGC AGGCCTCTCA 
GGCTTACATC GCACATACAG CCAGGACTGT AGCTTTAAAA ACAGCATGTA CCATGTTGGA 
GATTACGTCT ATGTGGAACC TGCAGAGGCC AACCTACAAC CACATATTGT CTGTATTGAA 
AGACTGTGGG AGGATTCAGC TGGTGAAAAA TGGTTGTATG GCTGTTGGTT TTACCGACCA 
AATGAAACAT TCCACCTGGC TACACGAAAA TTTCTAGAAA AAGAAGTTTT TAAGAGTGAC 
TATTACAACA AAGTTCCAGT TAGTAAAATT CTAGGCAAGT GTGTGGTCAT GTTTGTCAAG 
GAATACTTTA AGTTATGCCC AGAAAACTTC CGAGATGAGG ATGTTTTTGT CTGTGAATCA 
CGGTATTCTG CCAAAACCAA ATCTTTTAAG AAAATTAAAC TGTGGACCAT GCCCATCAGC 
TCAGTTAGGT TTGTCCCTCG GGATGTGCCT CTGCCTGTGG TTCGCGTGGC CTCTGTATTT 
GCAAATGCAG ATAAAGGTGA TGATGAGAAG AATACAGACA ACTCAGAGGA CAGTCGAGCT 
GAAGACAATT TTAACTTGGA AAAGGAAAAA GAAGATGTCC CTGTGGAAAT GTCCAATGGT 
GAACCAGGTT GCCACTACTT TGAGCAGCTC CATTACAATG ACATGTGGCT GAAGGTTGGC 
GACTGTGTCT TCATCAAGTC CCATGGCCTG GTGCGTCCTC GTGTGGGCAG AATTGAAAAA 
GTATGGGTTC GAGATGGAGC TGCATATTTT TATGGCCCCA TCTTCATTCA CCCAGAAGAA 
ACGGAGCATG AGCCCACAAA AATGTTCTAC AAAAAAGAAG TATTTCTGAG TAATCTGGAA 
GAAACCTGCC CCATGACATG TATTCTCGGA AAGTGTGCTG TGTTGTCATT CAAAGACTTC 
CTCTCCTGCA GGCCAACTGA AATACCAGAA AATGACATTC TGCTTTGTGA GAGCCGCTAC 
AATGAAAGTG ACAAGCAGAT GAAGAAATTC AAAGGATTGA AGAGGTTTTC ACTCTCTGCT 
AAAGTGGTAG ATGATGAAAT TTACTACTTC AGAAAACCAA TTGTTCCTCA GAAGGAGCCA 
TCACCTTTGT TGGAAAAGAA GATCCAGTTG CTGGAAGCTA AATTTGCCGA GTTAGAAGGT 
GGAGATGATG ATATTGAAGA GATGGGAGAA GAAGATAGTG AGGTCATTGA ACCTCCTTCT 
CTACCTCAGC TTCAGACCCC CCTGGCCAGT GAGCTGGACC TCATGCCCTA CACACCCCCA 
CAGTCTACCC CAAAGTCTGC CAAAGGCAGT GCAAAGAAGG AAGGCTCCAA ACGGAAAATC 
AACATGAGTG GCTACATCCT GTTCAGCAGT GAGATGAGGG CTGTGATTAA GGCCCAACAC 
CCAGATTACT CTTTCGGGGA GCTCAGCCGC CTGGTGGGGA CAGAATGGAG AAATCTTGAG 
ACAGCCAAGA AAGCGGAATA TGAAGGCATG ATGGGTGGCT ATCCGCCAGG CCTTCCACCT 
TTGCAGGGCC CAGTTGATGG CCTTGTTAGC ATGGGCAGCA TGCAGCCACT TCACCCTGGG 
GGGCCTCCAC CCCACCATCT TCCGCCAGGT GTGCCTGGCC TCCCGGGCAT CCCACCACCG 
GGTGTGATGA ACCAAGGAGT GGCCCCTATG GTAGGGACTC CAGCACCAGG TGGAAGTCCA 
TATGGACAAC AGGTGGGAGT TTTGGGGCCT CCAGGGCAGC AGGCACCACC TCCATATCCC 
GGCCCACATC CACCTGGACC CCCTGTCATA CAGCAGCCAA CAACACCCAT GTTTGTAGCT 
CCCCCACCAA AGACCCAGCG GCTTCTTCAC TCAGAGGCCT ACCTGAAATA CATTGAAGGA 
CTCAGTGCCG AGTCCAACAG CATTAGCAAG TGGGATCAGA CACTGGCAGC TCGAAGACGT 
GACGTCCATT TGTCAAAAGA ACAGGAGAGC CGCCTACCCT CTCACTGGCT GAAAAGCAAA 
GGGGCCCACA CCACCATGGC AGATGCCCTC TGGCGCCTTC GAGATTTGAT GCTGCGGGAC 
ACTCTCAACA TTCGCCAAGC ATACAACCTA GAAAACATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100165.1
CDS	327..5228
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKRAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244397.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244397.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGC TGAAAAGAGT GAAGATTCCT CTGGTGCTGC AGGCCTCTCA 
GGCTTACATC GCACATACAG CCAGGACTGT AGCTTTAAAA ACAGCATGTA CCATGTTGGA 
GATTACGTCT ATGTGGAACC TGCAGAGGCC AACCTACAAC CACATATTGT CTGTATTGAA 
AGACTGTGGG AGGATTCAGC TGGTGAAAAA TGGTTGTATG GCTGTTGGTT TTACCGACCA 
AATGAAACAT TCCACCTGGC TACACGAAAA TTTCTAGAAA AAGAAGTTTT TAAGAGTGAC 
TATTACAACA AAGTTCCAGT TAGTAAAATT CTAGGCAAGT GTGTGGTCAT GTTTGTCAAG 
GAATACTTTA AGTTATGCCC AGAAAACTTC CGAGATGAGG ATGTTTTTGT CTGTGAATCA 
CGGTATTCTG CCAAAACCAA ATCTTTTAAG AAAATTAAAC TGTGGACCAT GCCCATCAGC 
TCAGTTAGGT TTGTCCCTCG GGATGTGCCT CTGCCTGTGG TTCGCGTGGC CTCTGTATTT 
GCAAATGCAG ATAAAGGTGA TGATGAGAAG AATACAGACA ACTCAGAGGA CAGTCGAGCT 
GAAGACAATT TTAACTTGGA AAAGGAAAAA GAAGATGTCC CTGTGGAAAT GTCCAATGGT 
GAACCAGGTT GCCACTACTT TGAGCAGCTC CATTACAATG ACATGTGGCT GAAGGTTGGC 
GACTGTGTCT TCATCAAGTC CCATGGCCTG GTGCGTCCTC GTGTGGGCAG AATTGAAAAA 
GTATGGGTTC GAGATGGAGC TGCATATTTT TATGGCCCCA TCTTCATTCA CCCAGAAGAA 
ACGGAGCATG AGCCCACAAA AATGTTCTAC AAAAAAGAAG TATTTCTGAG TAATCTGGAA 
GAAACCTGCC CCATGACATG TATTCTCGGA AAGTGTGCTG TGTTGTCATT CAAAGACTTC 
CTCTCCTGCA GGCCAACTGA AATACCAGAA AATGACATTC TGCTTTGTGA GAGCCGCTAC 
AATGAAAGTG ACAAGCAGAT GAAGAAATTC AAAGGATTGA AGAGGTTTTC ACTCTCTGCT 
AAAGTGGTAG ATGATGAAAT TTACTACTTC AGAAAACCAA TTGTTCCTCA GAAGGAGCCA 
TCACCTTTGT TGGAAAAGAA GATCCAGTTG CTGGAAGCTA AATTTGCCGA GTTAGAAGGT 
GGAGATGATG ATATTGAAGA GATGGGAGAA GAAGATAGTG AGGTCATTGA ACCTCCTTCT 
CTACCTCAGC TTCAGACCCC CCTGGCCAGT GAGCTGGACC TCATGCCCTA CACACCCCCA 
CAGTCTACCC CAAAGTCTGC CAAAGGCAGT GCAAAGAAGG AAGGCTCCAA ACGGAAAATC 
AACATGAGTG GCTACATCCT GTTCAGCAGT GAGATGAGGG CTGTGATTAA GGCCCAACAC 
CCAGATTACT CTTTCGGGGA GCTCAGCCGC CTGGTGGGGA CAGAATGGAG AAATCTTGAG 
ACAGCCAAGA AAGCGGAATA TGAAGGCATG ATGGGTGGCT ATCCGCCAGG CCTTCCACCT 
TTGCAGGGCC CAGTTGATGG CCTTGTTAGC ATGGGCAGCA TGCAGCCACT TCACCCTGGG 
GGGCCTCCAC CCCACCATCT TCCGCCAGGT GTGCCTGGCC TCCCGGGCAT CCCACCACCG 
GGTGTGATGA ACCAAGGAGT GGCCCCTATG GTAGGGACTC CAGCACCAGG TGGAAGTCCA 
TATGGACAAC AGGTGGGAGT TTTGGGGCCT CCAGGGCAGC AGGCACCACC TCCATATCCC 
GGCCCACATC CACCTGGACC CCCTGTCATA CAGCAGCCAA CAACACCCAT GTTTGTAGCT 
CCCCCACCAA AGACCCAGCG GCTTCTTCAC TCAGAGGCCT ACCTGAAATA CATTGAAGGA 
CTCAGTGCCG AGTCCAACAG CATTAGCAAG TGGGATCAGA CACTGGCAGC TCGAAGACGT 
GACGTCCATT TGTCAAAAGA ACAGGAGAGC CGCCTACCCT CTCACTGGCT GAAAAGCAAA 
GGGGCCCACA CCACCATGGC AGATGCCCTC TGGCGCCTTC GAGATTTGAT GCTGCGGGAC 
ACTCTCAACA TTCGCCAAGC ATACAACCTA GAAAACATTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244398.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100166.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OSa179456
Clone ID Related Accession (Same CDS sequence)	XM_024244398.1
Accession Version	XM_024244398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGGTGTGAT GAACCAAGGA GTGGCCCCTA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CATATGGACA ACAGGTGGGA GTTTTGGGGC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CCGGCCCACA TCCACCTGGA CCCCCTGTCA TACAGCAGCC AACAACACCC 
ATGTTTGTAG CTCCCCCACC AAAGACCCAG CGGCTTCTTC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC CGAGTCCAAC AGCATTAGCA AGTGGGATCA GACACTGGCA 
GCTCGAAGAC GTGACGTCCA TTTGTCAAAA GAACAGGAGA GCCGCCTACC CTCTCACTGG 
CTGAAAAGCA AAGGGGCCCA CACCACCATG GCAGATGCCC TCTGGCGCCT TCGAGATTTG 
ATGCTGCGGG ACACTCTCAA CATTCGCCAA GCATACAACC TAGAAAACAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100166.1
CDS	327..5120
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244398.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244398.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGGTGTGAT GAACCAAGGA GTGGCCCCTA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CATATGGACA ACAGGTGGGA GTTTTGGGGC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CCGGCCCACA TCCACCTGGA CCCCCTGTCA TACAGCAGCC AACAACACCC 
ATGTTTGTAG CTCCCCCACC AAAGACCCAG CGGCTTCTTC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC CGAGTCCAAC AGCATTAGCA AGTGGGATCA GACACTGGCA 
GCTCGAAGAC GTGACGTCCA TTTGTCAAAA GAACAGGAGA GCCGCCTACC CTCTCACTGG 
CTGAAAAGCA AAGGGGCCCA CACCACCATG GCAGATGCCC TCTGGCGCCT TCGAGATTTG 
ATGCTGCGGG ACACTCTCAA CATTCGCCAA GCATACAACC TAGAAAACAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244399.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100167.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X16, mRNA.

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CloneID	OSa179457
Clone ID Related Accession (Same CDS sequence)	XM_024244399.1
Accession Version	XM_024244399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGCTGAAA AGAGTGAAGA TTCCTCTGGT GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGTAGCTT TAAAAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAACCTGCAG AGGCCAACCT ACAACCACAT ATTGTCTGTA TTGAAAGACT GTGGGAGGAT 
TCAGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTACC GACCAAATGA AACATTCCAC 
CTGGCTACAC GAAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAACAAAGTT 
CCAGTTAGTA AAATTCTAGG CAAGTGTGTG GTCATGTTTG TCAAGGAATA CTTTAAGTTA 
TGCCCAGAAA ACTTCCGAGA TGAGGATGTT TTTGTCTGTG AATCACGGTA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCA TCAGCTCAGT TAGGTTTGTC 
CCTCGGGATG TGCCTCTGCC TGTGGTTCGC GTGGCCTCTG TATTTGCAAA TGCAGATAAA 
GGTGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAGCTGAAGA CAATTTTAAC 
TTGGAAAAGG AAAAAGAAGA TGTCCCTGTG GAAATGTCCA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCATTA CAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GCCTGGTGCG TCCTCGTGTG GGCAGAATTG AAAAAGTATG GGTTCGAGAT 
GGAGCTGCAT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAAACGGA GCATGAGCCC 
ACAAAAATGT TCTACAAAAA AGAAGTATTT CTGAGTAATC TGGAAGAAAC CTGCCCCATG 
ACATGTATTC TCGGAAAGTG TGCTGTGTTG TCATTCAAAG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATAC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAATGA AAGTGACAAG 
CAGATGAAGA AATTCAAAGG ATTGAAGAGG TTTTCACTCT CTGCTAAAGT GGTAGATGAT 
GAAATTTACT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCATCACC TTTGTTGGAA 
AAGAAGATCC AGTTGCTGGA AGCTAAATTT GCCGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAAGA TAGTGAGGTC ATTGAACCTC CTTCTCTACC TCAGCTTCAG 
ACCCCCCTGG CCAGTGAGCT GGACCTCATG CCCTACACAC CCCCACAGTC TACCCCAAAG 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AAATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGGGCTGTG ATTAAGGCCC AACACCCAGA TTACTCTTTC 
GGGGAGCTCA GCCGCCTGGT GGGGACAGAA TGGAGAAATC TTGAGACAGC CAAGAAAGCG 
GAATATGAAG GTGTGATGAA CCAAGGAGTG GCCCCTATGG TAGGGACTCC AGCACCAGGT 
GGAAGTCCAT ATGGACAACA GGTGGGAGTT TTGGGGCCTC CAGGGCAGCA GGCACCACCT 
CCATATCCCG GCCCACATCC ACCTGGACCC CCTGTCATAC AGCAGCCAAC AACACCCATG 
TTTGTAGCTC CCCCACCAAA GACCCAGCGG CTTCTTCACT CAGAGGCCTA CCTGAAATAC 
ATTGAAGGAC TCAGTGCCGA GTCCAACAGC ATTAGCAAGT GGGATCAGAC ACTGGCAGCT 
CGAAGACGTG ACGTCCATTT GTCAAAAGAA CAGGAGAGCC GCCTACCCTC TCACTGGCTG 
AAAAGCAAAG GGGCCCACAC CACCATGGCA GATGCCCTCT GGCGCCTTCG AGATTTGATG 
CTGCGGGACA CTCTCAACAT TCGCCAAGCA TACAACCTAG AAAACATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100167.1
CDS	327..5117
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKRAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244399.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244399.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGCTGAAA AGAGTGAAGA TTCCTCTGGT GCTGCAGGCC TCTCAGGCTT ACATCGCACA 
TACAGCCAGG ACTGTAGCTT TAAAAACAGC ATGTACCATG TTGGAGATTA CGTCTATGTG 
GAACCTGCAG AGGCCAACCT ACAACCACAT ATTGTCTGTA TTGAAAGACT GTGGGAGGAT 
TCAGCTGGTG AAAAATGGTT GTATGGCTGT TGGTTTTACC GACCAAATGA AACATTCCAC 
CTGGCTACAC GAAAATTTCT AGAAAAAGAA GTTTTTAAGA GTGACTATTA CAACAAAGTT 
CCAGTTAGTA AAATTCTAGG CAAGTGTGTG GTCATGTTTG TCAAGGAATA CTTTAAGTTA 
TGCCCAGAAA ACTTCCGAGA TGAGGATGTT TTTGTCTGTG AATCACGGTA TTCTGCCAAA 
ACCAAATCTT TTAAGAAAAT TAAACTGTGG ACCATGCCCA TCAGCTCAGT TAGGTTTGTC 
CCTCGGGATG TGCCTCTGCC TGTGGTTCGC GTGGCCTCTG TATTTGCAAA TGCAGATAAA 
GGTGATGATG AGAAGAATAC AGACAACTCA GAGGACAGTC GAGCTGAAGA CAATTTTAAC 
TTGGAAAAGG AAAAAGAAGA TGTCCCTGTG GAAATGTCCA ATGGTGAACC AGGTTGCCAC 
TACTTTGAGC AGCTCCATTA CAATGACATG TGGCTGAAGG TTGGCGACTG TGTCTTCATC 
AAGTCCCATG GCCTGGTGCG TCCTCGTGTG GGCAGAATTG AAAAAGTATG GGTTCGAGAT 
GGAGCTGCAT ATTTTTATGG CCCCATCTTC ATTCACCCAG AAGAAACGGA GCATGAGCCC 
ACAAAAATGT TCTACAAAAA AGAAGTATTT CTGAGTAATC TGGAAGAAAC CTGCCCCATG 
ACATGTATTC TCGGAAAGTG TGCTGTGTTG TCATTCAAAG ACTTCCTCTC CTGCAGGCCA 
ACTGAAATAC CAGAAAATGA CATTCTGCTT TGTGAGAGCC GCTACAATGA AAGTGACAAG 
CAGATGAAGA AATTCAAAGG ATTGAAGAGG TTTTCACTCT CTGCTAAAGT GGTAGATGAT 
GAAATTTACT ACTTCAGAAA ACCAATTGTT CCTCAGAAGG AGCCATCACC TTTGTTGGAA 
AAGAAGATCC AGTTGCTGGA AGCTAAATTT GCCGAGTTAG AAGGTGGAGA TGATGATATT 
GAAGAGATGG GAGAAGAAGA TAGTGAGGTC ATTGAACCTC CTTCTCTACC TCAGCTTCAG 
ACCCCCCTGG CCAGTGAGCT GGACCTCATG CCCTACACAC CCCCACAGTC TACCCCAAAG 
TCTGCCAAAG GCAGTGCAAA GAAGGAAGGC TCCAAACGGA AAATCAACAT GAGTGGCTAC 
ATCCTGTTCA GCAGTGAGAT GAGGGCTGTG ATTAAGGCCC AACACCCAGA TTACTCTTTC 
GGGGAGCTCA GCCGCCTGGT GGGGACAGAA TGGAGAAATC TTGAGACAGC CAAGAAAGCG 
GAATATGAAG GTGTGATGAA CCAAGGAGTG GCCCCTATGG TAGGGACTCC AGCACCAGGT 
GGAAGTCCAT ATGGACAACA GGTGGGAGTT TTGGGGCCTC CAGGGCAGCA GGCACCACCT 
CCATATCCCG GCCCACATCC ACCTGGACCC CCTGTCATAC AGCAGCCAAC AACACCCATG 
TTTGTAGCTC CCCCACCAAA GACCCAGCGG CTTCTTCACT CAGAGGCCTA CCTGAAATAC 
ATTGAAGGAC TCAGTGCCGA GTCCAACAGC ATTAGCAAGT GGGATCAGAC ACTGGCAGCT 
CGAAGACGTG ACGTCCATTT GTCAAAAGAA CAGGAGAGCC GCCTACCCTC TCACTGGCTG 
AAAAGCAAAG GGGCCCACAC CACCATGGCA GATGCCCTCT GGCGCCTTCG AGATTTGATG 
CTGCGGGACA CTCTCAACAT TCGCCAAGCA TACAACCTAG AAAACATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244400.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100168.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X17, mRNA.

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CloneID	OSa179458
Clone ID Related Accession (Same CDS sequence)	XM_024244400.1 , XM_024244401.1
Accession Version	XM_024244400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCATAT GGACAACAGG TGGGAGTTTT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCCGGC CCACATCCAC CTGGACCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTAGCTCCC CCACCAAAGA CCCAGCGGCT TCTTCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCCGAGT CCAACAGCAT TAGCAAGTGG 
GATCAGACAC TGGCAGCTCG AAGACGTGAC GTCCATTTGT CAAAAGAACA GGAGAGCCGC 
CTACCCTCTC ACTGGCTGAA AAGCAAAGGG GCCCACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTTCGAG ATTTGATGCT GCGGGACACT CTCAACATTC GCCAAGCATA CAACCTAGAA 
AACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100168.1
CDS	327..5075
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244400.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244400.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCATAT GGACAACAGG TGGGAGTTTT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCCGGC CCACATCCAC CTGGACCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTAGCTCCC CCACCAAAGA CCCAGCGGCT TCTTCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCCGAGT CCAACAGCAT TAGCAAGTGG 
GATCAGACAC TGGCAGCTCG AAGACGTGAC GTCCATTTGT CAAAAGAACA GGAGAGCCGC 
CTACCCTCTC ACTGGCTGAA AAGCAAAGGG GCCCACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTTCGAG ATTTGATGCT GCGGGACACT CTCAACATTC GCCAAGCATA CAACCTAGAA 
AACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244401.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100169.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X18, mRNA.

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CloneID	OSa179458
Clone ID Related Accession (Same CDS sequence)	XM_024244400.1 , XM_024244401.1
Accession Version	XM_024244401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCATAT GGACAACAGG TGGGAGTTTT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCCGGC CCACATCCAC CTGGACCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTAGCTCCC CCACCAAAGA CCCAGCGGCT TCTTCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCCGAGT CCAACAGCAT TAGCAAGTGG 
GATCAGACAC TGGCAGCTCG AAGACGTGAC GTCCATTTGT CAAAAGAACA GGAGAGCCGC 
CTACCCTCTC ACTGGCTGAA AAGCAAAGGG GCCCACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTTCGAG ATTTGATGCT GCGGGACACT CTCAACATTC GCCAAGCATA CAACCTAGAA 
AACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100169.1
CDS	187..4935
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244401.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244401.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGGTGAA AAATGGTTGT ATGGCTGTTG GTTTTACCGA 
CCAAATGAAA CATTCCACCT GGCTACACGA AAATTTCTAG AAAAAGAAGT TTTTAAGAGT 
GACTATTACA ACAAAGTTCC AGTTAGTAAA ATTCTAGGCA AGTGTGTGGT CATGTTTGTC 
AAGGAATACT TTAAGTTATG CCCAGAAAAC TTCCGAGATG AGGATGTTTT TGTCTGTGAA 
TCACGGTATT CTGCCAAAAC CAAATCTTTT AAGAAAATTA AACTGTGGAC CATGCCCATC 
AGCTCAGTTA GGTTTGTCCC TCGGGATGTG CCTCTGCCTG TGGTTCGCGT GGCCTCTGTA 
TTTGCAAATG CAGATAAAGG TGATGATGAG AAGAATACAG ACAACTCAGA GGACAGTCGA 
GCTGAAGACA ATTTTAACTT GGAAAAGGAA AAAGAAGATG TCCCTGTGGA AATGTCCAAT 
GGTGAACCAG GTTGCCACTA CTTTGAGCAG CTCCATTACA ATGACATGTG GCTGAAGGTT 
GGCGACTGTG TCTTCATCAA GTCCCATGGC CTGGTGCGTC CTCGTGTGGG CAGAATTGAA 
AAAGTATGGG TTCGAGATGG AGCTGCATAT TTTTATGGCC CCATCTTCAT TCACCCAGAA 
GAAACGGAGC ATGAGCCCAC AAAAATGTTC TACAAAAAAG AAGTATTTCT GAGTAATCTG 
GAAGAAACCT GCCCCATGAC ATGTATTCTC GGAAAGTGTG CTGTGTTGTC ATTCAAAGAC 
TTCCTCTCCT GCAGGCCAAC TGAAATACCA GAAAATGACA TTCTGCTTTG TGAGAGCCGC 
TACAATGAAA GTGACAAGCA GATGAAGAAA TTCAAAGGAT TGAAGAGGTT TTCACTCTCT 
GCTAAAGTGG TAGATGATGA AATTTACTAC TTCAGAAAAC CAATTGTTCC TCAGAAGGAG 
CCATCACCTT TGTTGGAAAA GAAGATCCAG TTGCTGGAAG CTAAATTTGC CGAGTTAGAA 
GGTGGAGATG ATGATATTGA AGAGATGGGA GAAGAAGATA GTGAGGTCAT TGAACCTCCT 
TCTCTACCTC AGCTTCAGAC CCCCCTGGCC AGTGAGCTGG ACCTCATGCC CTACACACCC 
CCACAGTCTA CCCCAAAGTC TGCCAAAGGC AGTGCAAAGA AGGAAGGCTC CAAACGGAAA 
ATCAACATGA GTGGCTACAT CCTGTTCAGC AGTGAGATGA GGGCTGTGAT TAAGGCCCAA 
CACCCAGATT ACTCTTTCGG GGAGCTCAGC CGCCTGGTGG GGACAGAATG GAGAAATCTT 
GAGACAGCCA AGAAAGCGGA ATATGAAGGT GTGATGAACC AAGGAGTGGC CCCTATGGTA 
GGGACTCCAG CACCAGGTGG AAGTCCATAT GGACAACAGG TGGGAGTTTT GGGGCCTCCA 
GGGCAGCAGG CACCACCTCC ATATCCCGGC CCACATCCAC CTGGACCCCC TGTCATACAG 
CAGCCAACAA CACCCATGTT TGTAGCTCCC CCACCAAAGA CCCAGCGGCT TCTTCACTCA 
GAGGCCTACC TGAAATACAT TGAAGGACTC AGTGCCGAGT CCAACAGCAT TAGCAAGTGG 
GATCAGACAC TGGCAGCTCG AAGACGTGAC GTCCATTTGT CAAAAGAACA GGAGAGCCGC 
CTACCCTCTC ACTGGCTGAA AAGCAAAGGG GCCCACACCA CCATGGCAGA TGCCCTCTGG 
CGCCTTCGAG ATTTGATGCT GCGGGACACT CTCAACATTC GCCAAGCATA CAACCTAGAA 
AACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244403.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100171.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OSa179459
Clone ID Related Accession (Same CDS sequence)	XM_024244403.1
Accession Version	XM_024244403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGGTGTGAT GAACCAAGGA GTGGCCCCTA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CATATGGACA ACAGGTGGGA GTTTTGGGGC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CCGGCCCACA TCCACCTGGA CCCCCTGTCA TACAGCAGCC AACAACACCC 
ATGTTTGTAG CTCCCCCACC AAAGACCCAG CGGCTTCTTC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC CGAGTCCAAC AGCATTAGCA AGTGGGATCA GACACTGGCA 
GCTCGAAGAC GTGACGTCCA TTTGTCAAAA GAACAGGAGA GCCGCCTACC CTCTCACTGG 
CTGAAAAGCA AAGGGGCCCA CACCACCATG GCAGATGCCC TCTGGCGCCT TCGAGATTTG 
ATGCTGCGGG ACACTCTCAA CATTCGCCAA GCATACAACC TAGAAAACAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100171.1
CDS	327..5000
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244403.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244403.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGGTGTGAT GAACCAAGGA GTGGCCCCTA TGGTAGGGAC TCCAGCACCA 
GGTGGAAGTC CATATGGACA ACAGGTGGGA GTTTTGGGGC CTCCAGGGCA GCAGGCACCA 
CCTCCATATC CCGGCCCACA TCCACCTGGA CCCCCTGTCA TACAGCAGCC AACAACACCC 
ATGTTTGTAG CTCCCCCACC AAAGACCCAG CGGCTTCTTC ACTCAGAGGC CTACCTGAAA 
TACATTGAAG GACTCAGTGC CGAGTCCAAC AGCATTAGCA AGTGGGATCA GACACTGGCA 
GCTCGAAGAC GTGACGTCCA TTTGTCAAAA GAACAGGAGA GCCGCCTACC CTCTCACTGG 
CTGAAAAGCA AAGGGGCCCA CACCACCATG GCAGATGCCC TCTGGCGCCT TCGAGATTTG 
ATGCTGCGGG ACACTCTCAA CATTCGCCAA GCATACAACC TAGAAAACAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_024244404.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024100172.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OSa179460
Clone ID Related Accession (Same CDS sequence)	XM_024244404.1
Accession Version	XM_024244404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036906.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCATA TGGACAACAG 
GTGGGAGTTT TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCCGG CCCACATCCA 
CCTGGACCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTAGCTCC CCCACCAAAG 
ACCCAGCGGC TTCTTCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCCGAG 
TCCAACAGCA TTAGCAAGTG GGATCAGACA CTGGCAGCTC GAAGACGTGA CGTCCATTTG 
TCAAAAGAAC AGGAGAGCCG CCTACCCTCT CACTGGCTGA AAAGCAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTTCGA GATTTGATGC TGCGGGACAC TCTCAACATT 
CGCCAAGCAT ACAACCTAGA AAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024100172.1
CDS	210..5039
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIVPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_024244404.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024244404.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAACGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GTAAAAAGAA CATAAGAAAG 
CAGCGAATGA AAATCTTATT CAATGTTGTT CTTGAAGCTC GAGAGCCAGG TTCAGGCAGA 
AGACTTTGTG ACCTATTTAT GGTTAAACCA TCCAAAAAGG ACTATCCTGA TTATTATAAA 
ATCATCTTGG AGCCAATGGA CTTGAAAATA ATTGAGCATA ACATCCGCAA TGACAAATAT 
GCCGGTGAAG AGGGAATGAT AGAAGACATG AAGCTGATGT TCCGGAATGC CAGGCACTAT 
AACGAGGAGG GCTCCCAGGT ATACAATGAT GCACATATCC TGGAGAAGTT ACTCAAGGAG 
AAAAGGAAAG AGCTGGGCCC ACTGCCTGAT GATGATGACA TGGCTTCTCC CAAACTCAAG 
CTGAGTAGGA AGAGTGGCAT TTCTCCTAAA AAATCAAAAT ACATGACTCC AATGCAGCAG 
AAACTAAATG AGGTCTATGA AGCTGTAAAG AACTATACTG ATAAGAGGGG TCGCCGCCTC 
AGTGCCATAT TTCTGAGGCT TCCCTCTAGA TCTGAGTTGC CTGACTACTA TCTGACTATT 
AAAAAGCCCA TGGACATGGA AAAAATTCGA AGTCACATGA TGGCCAACAA GTACCAAGAT 
ATTGACTCTA TGGTTGAGGA CTTTGTCATG ATGTTTAATA ATGCCTGTAC ATACAATGAG 
CCTGAGTCTT TGATCTACAA AGATGCTCTT GTTCTACACA AAGTCCTGCT TGAAACACGC 
AGAGACCTGG AGGGAGATGA GGACTCTCAT GTCCCAAATG TGACTTTGCT GATTCAAGAG 
CTTATCCACA ATCTTTTTGT GTCAGTCATG AGTCATCAGG ATGATGAGGG AAGATGCTAC 
AGTGATTCTT TAGCAGAAAT TCCTGCTGTG GATCCCAACT TTCCTAACAA ACCACCCCTT 
ACATTTGACA TAATTAGGAA GAATGTTGAA AATAATCGCT ACCGGCGGCT TGATTTATTT 
CAAGAGCATA TGTTTGAAGT ATTGGAACGA GCAAGAAGGA TGAATCGGAC AGATTCAGAA 
ATATATGAAG ATGCGGTAGA ACTTCAGCAG TTTTTTATTA AAATTCGTGA TGAACTCTGC 
AAAAATGGAG AGATTCTTCT TTCACCGGCA CTCAGCTATA CCACAAAACA TTTGCATAAT 
GATGTGGAGA AAGAGAAAAA GGAAAAATTG CCAAAAGAAA TAGAGGAAGA TAAACTAAAA 
CGAGAAGAAG AAAAAAGAGA AGCTGAAAAG AGTGAAGATT CCTCTGGTGC TGCAGGCCTC 
TCAGGCTTAC ATCGCACATA CAGCCAGGAC TGTAGCTTTA AAAACAGCAT GTACCATGTT 
GGAGATTACG TCTATGTGGA ACCTGCAGAG GCCAACCTAC AACCACATAT TGTCTGTATT 
GAAAGACTGT GGGAGGATTC AGCTGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT GTTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGGCATGAT GGGTGGCTAT CCGCCAGGCC TTCCACCTTT GCAGGGCCCA 
GTTGATGGCC TTGTTAGCAT GGGCAGCATG CAGCCACTTC ACCCTGGGGG GCCTCCACCC 
CACCATCTTC CGCCAGGTGT GCCTGGCCTC CCGGGCATCC CACCACCGGG TGTGATGAAC 
CAAGGAGTGG CCCCTATGGT AGGGACTCCA GCACCAGGTG GAAGTCCATA TGGACAACAG 
GTGGGAGTTT TGGGGCCTCC AGGGCAGCAG GCACCACCTC CATATCCCGG CCCACATCCA 
CCTGGACCCC CTGTCATACA GCAGCCAACA ACACCCATGT TTGTAGCTCC CCCACCAAAG 
ACCCAGCGGC TTCTTCACTC AGAGGCCTAC CTGAAATACA TTGAAGGACT CAGTGCCGAG 
TCCAACAGCA TTAGCAAGTG GGATCAGACA CTGGCAGCTC GAAGACGTGA CGTCCATTTG 
TCAAAAGAAC AGGAGAGCCG CCTACCCTCT CACTGGCTGA AAAGCAAAGG GGCCCACACC 
ACCATGGCAG ATGCCCTCTG GCGCCTTCGA GATTTGATGC TGCGGGACAC TCTCAACATT 
CGCCAAGCAT ACAACCTAGA AAACATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_009238921.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009237196.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X1, mRNA.

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CloneID	OSa34489
Clone ID Related Accession (Same CDS sequence)	XM_009238921.1 , XM_009238923.1 , XM_009238922.1 , XM_009238924.1
Accession Version	XM_009238921.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002877572.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT ATTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009237196.1
CDS	239..5353
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_009238921.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009238921.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT ATTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_009238923.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009237198.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X3, mRNA.

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CloneID	OSa34489
Clone ID Related Accession (Same CDS sequence)	XM_009238921.1 , XM_009238923.1 , XM_009238922.1 , XM_009238924.1
Accession Version	XM_009238923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002877572.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT ATTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009237198.1
CDS	95..5209
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_009238923.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009238923.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT ATTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_009238922.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009237197.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X2, mRNA.

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CloneID	OSa34489
Clone ID Related Accession (Same CDS sequence)	XM_009238921.1 , XM_009238923.1 , XM_009238922.1 , XM_009238924.1
Accession Version	XM_009238922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002877572.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT ATTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009237197.1
CDS	297..5411
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_009238922.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009238922.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT ATTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PBRM1 ( XM_009238924.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009237199.1 Pongo abelii polybromo 1 (PBRM1), transcript variant X4, mRNA.

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CloneID	OSa34489
Clone ID Related Accession (Same CDS sequence)	XM_009238921.1 , XM_009238923.1 , XM_009238922.1 , XM_009238924.1
Accession Version	XM_009238924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	protein polybromo-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002877572.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT ATTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009237199.1
CDS	181..5295
Translation	MGSKRRRATSPSSSVSGDFDDGHHSVSTPGPSRKRRRLSNLPTVDPIAVCHELYNTIRDYKDEQGRLLCELFIRAPKRRNQPDYYEVVSQPIDLMKIQQKLKMEEYDDVNLLTADFQLLFNNAKAYYKPDSPEYKAACKLWDLYLRTRNEFVQKGEADDEDDDEDGQDNQGTVTEGSSPTYLKEILEQLLEAIVVATNPSGRLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHAMAKDIDLLAKNAKTYNEPGSQVFKDANSIKKIFYMKKAEIEHHEMAKSSLRMRTPSNLAAARLTGPSHSKGSLGEERNPTSKYYRNKRAVQGGRLSAITMALQYGSESEEDAALAAARYEEGESEAESITSFMDVSNPFYQLYDTVRSCRNNQGQLIAEPFFHLPSKKKYPDYYQQIKMPISLQQIRTKLKNQEYETLDHLECDLNLMFENAKRYNVPNSAIYKRVLKLQQVMQAKKKELARRDDIEDGDSMISSATSDTGSAKRKRNTHDSEMLGLRRLSSKKNIRKQRMKILFNVVLEAREPGSGRRLCDLFMVKPSKKDYPDYYKIILEPMDLKIIEHNIRNDKYAGEEGMIEDMKLMFRNARHYNEEGSQVYNDAHILEKLLKEKRKELGPLPDDDDMASPKLKLSRKSGISPKKSKYMTPMQQKLNEVYEAVKNYTDKRGRRLSAIFLRLPSRSELPDYYLTIKKPMDMEKIRSHMMANKYQDIDSMVEDFVMMFNNACTYNEPESLIYKDALVLHKVLLETRRDLEGDEDSHVPNVTLLIQELIHNLFVSVMSHQDDEGRCYSDSLAEIPAVDPNFPNKPPLTFDIIRKNVENNRYRRLDLFQEHMFEVLERARRMNRTDSEIYEDAVELQQFFIKIRDELCKNGEILLSPALSYTTKHLHNDVEKEKKEKLPKEIEEDKLKREEEKREAEKSEDSSGAAGLSGLHRTYSQDCSFKNSMYHVGDYVYVEPAEANLQPHIVCIERLWEDSAGEKWLYGCWFYRPNETFHLATRKFLEKEVFKSDYYNKVPVSKILGKCVVMFVKEYFKLCPENFRDEDVFVCESRYSAKTKSFKKIKLWTMPISSVRFVPRDVPLPVVRVASVFANADKGDDEKNTDNSEDSRAEDNFNLEKEKEDVPVEMSNGEPGCHYFEQLHYNDMWLKVGDCVFIKSHGLVRPRVGRIEKVWVRDGAAYFYGPIFIHPEETEHEPTKMFYKKEVFLSNLEETCPMTCILGKCAVLSFKDFLSCRPTEIPENDILLCESRYNESDKQMKKFKGLKRFSLSAKVVDDEIYYFRKPIIPQKEPSPLLEKKIQLLEAKFAELEGGDDDIEEMGEEDSEVIEPPSLPQLQTPLASELDLMPYTPPQSTPKSAKGSAKKEGSKRKINMSGYILFSSEMRAVIKAQHPDYSFGELSRLVGTEWRNLETAKKAEYEERAAKVAEQQERERAAQQQQPSASPRAGTPVGALMGVVPPPTPMGMLNQQLTPVAGMMGGYPPGLPPLQGPVDGLVSMGSMQPLHPGGPPPHHLPPGVPGLPGIPPPGVMNQGVAPMVGTPAPGGSPYGQQVGVLGPPGQQAPPPYPGPHPPGPPVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAARRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIRQAYNLENI

Target ORF information:

RefSeq Version	XM_009238924.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii polybromo 1 (PBRM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009238924.1

ORF Insert Sequence:

ATGGGTTCCA AGAGAAGAAG AGCTACCTCC CCTTCCAGCA GTGTCAGCGG GGACTTTGAT 
GATGGGCACC ATTCTGTGTC AACACCAGGC CCAAGCAGGA AAAGGAGGAG ACTTTCCAAT 
CTTCCAACCG TAGATCCTAT TGCCGTGTGC CATGAACTCT ATAATACCAT CCGAGACTAT 
AAGGATGAAC AGGGCAGACT TCTCTGTGAG CTCTTCATTA GGGCACCAAA GCGAAGAAAT 
CAACCAGACT ATTATGAAGT GGTTTCTCAG CCCATTGACT TGATGAAAAT CCAACAGAAA 
CTAAAAATGG AAGAGTATGA TGATGTTAAT TTGCTGACTG CTGACTTCCA GCTTCTTTTT 
AACAATGCAA AGGCCTATTA TAAGCCAGAT TCTCCTGAAT ATAAAGCCGC TTGCAAACTC 
TGGGATTTGT ACCTTCGAAC AAGAAATGAG TTTGTTCAGA AAGGAGAAGC AGATGACGAA 
GATGATGATG AAGATGGGCA AGACAATCAG GGCACAGTGA CTGAAGGATC TTCTCCAACT 
TACTTGAAGG AGATCCTGGA GCAGCTTCTT GAAGCCATAG TTGTAGCTAC AAATCCATCA 
GGACGTCTCA TTAGCGAACT TTTTCAGAAA CTGCCTTCTA AAGTGCAATA TCCAGATTAT 
TATGCAATAA TTAAGGAGCC TATAGATCTC AAGACCATTG CCCAGAGGAT ACAGAATGGA 
AGCTACAAAA GTATTCATGC AATGGCCAAA GATATAGATC TCCTCGCAAA AAATGCCAAA 
ACTTATAATG AGCCTGGCTC TCAAGTATTC AAGGATGCAA ATTCAATTAA AAAAATATTT 
TATATGAAAA AGGCTGAAAT TGAACATCAT GAAATGGCTA AGTCAAGTCT TCGAATGAGG 
ACTCCATCCA ACTTGGCTGC AGCCAGACTG ACAGGTCCTT CACACAGTAA AGGCAGCCTT 
GGTGAAGAGA GAAATCCCAC TAGCAAGTAT TACCGTAATA AAAGAGCAGT ACAAGGAGGT 
CGTTTATCAG CAATTACAAT GGCACTTCAA TATGGCTCAG AAAGTGAAGA AGATGCTGCT 
TTAGCTGCTG CACGTTATGA AGAGGGAGAG TCAGAAGCAG AAAGCATCAC TTCCTTTATG 
GATGTTTCAA ATCCTTTTTA TCAGCTTTAT GACACAGTTA GGAGTTGTCG GAATAACCAA 
GGGCAGCTAA TAGCTGAACC TTTTTTCCAT TTGCCTTCAA AGAAAAAATA CCCTGATTAT 
TACCAGCAAA TTAAAATGCC CATATCACTA CAACAGATCC GAACAAAGCT GAAGAATCAA 
GAATATGAAA CTTTAGATCA TTTGGAGTGT GATCTGAATT TAATGTTTGA AAATGCCAAA 
CGCTATAATG TGCCCAATTC AGCCATCTAC AAGCGAGTTC TAAAATTGCA GCAAGTTATG 
CAGGCAAAGA AGAAAGAGCT TGCCAGGAGA GACGATATCG AGGACGGAGA CAGCATGATC 
TCTTCAGCCA CCTCTGATAC TGGTAGTGCC AAAAGAAAAA GGAACACTCA TGACAGTGAG 
ATGTTGGGTC TCAGGAGGCT ATCCAGTAAA AAGAACATAA GAAAGCAGCG AATGAAAATC 
TTATTCAATG TTGTTCTTGA AGCTCGAGAG CCAGGTTCAG GCAGAAGACT TTGTGACCTA 
TTTATGGTTA AACCATCCAA AAAGGACTAT CCTGATTATT ATAAAATCAT CTTGGAGCCA 
ATGGACTTGA AAATAATTGA GCATAACATC CGCAATGACA AATATGCCGG TGAAGAGGGA 
ATGATAGAAG ACATGAAGCT GATGTTCCGG AATGCCAGGC ACTATAACGA GGAGGGCTCC 
CAGGTATACA ATGATGCACA TATCCTGGAG AAGTTACTCA AGGAGAAAAG GAAAGAGCTG 
GGCCCACTGC CTGATGATGA TGACATGGCT TCTCCCAAAC TCAAGCTGAG TAGGAAGAGT 
GGCATTTCTC CTAAAAAATC AAAATACATG ACTCCAATGC AGCAGAAACT AAATGAGGTC 
TATGAAGCTG TAAAGAACTA TACTGATAAG AGGGGTCGCC GCCTCAGTGC CATATTTCTG 
AGGCTTCCCT CTAGATCTGA GTTGCCTGAC TACTATCTGA CTATTAAAAA GCCCATGGAC 
ATGGAAAAAA TTCGAAGTCA CATGATGGCC AACAAGTACC AAGATATTGA CTCTATGGTT 
GAGGACTTTG TCATGATGTT TAATAATGCC TGTACATACA ATGAGCCTGA GTCTTTGATC 
TACAAAGATG CTCTTGTTCT ACACAAAGTC CTGCTTGAAA CACGCAGAGA CCTGGAGGGA 
GATGAGGACT CTCATGTCCC AAATGTGACT TTGCTGATTC AAGAGCTTAT CCACAATCTT 
TTTGTGTCAG TCATGAGTCA TCAGGATGAT GAGGGAAGAT GCTACAGTGA TTCTTTAGCA 
GAAATTCCTG CTGTGGATCC CAACTTTCCT AACAAACCAC CCCTTACATT TGACATAATT 
AGGAAGAATG TTGAAAATAA TCGCTACCGG CGGCTTGATT TATTTCAAGA GCATATGTTT 
GAAGTATTGG AACGAGCAAG AAGGATGAAT CGGACAGATT CAGAAATATA TGAAGATGCG 
GTAGAACTTC AGCAGTTTTT TATTAAAATT CGTGATGAAC TCTGCAAAAA TGGAGAGATT 
CTTCTTTCAC CGGCACTCAG CTATACCACA AAACATTTGC ATAATGATGT GGAGAAAGAG 
AAAAAGGAAA AATTGCCAAA AGAAATAGAG GAAGATAAAC TAAAACGAGA AGAAGAAAAA 
AGAGAAGCTG AAAAGAGTGA AGATTCCTCT GGTGCTGCAG GCCTCTCAGG CTTACATCGC 
ACATACAGCC AGGACTGTAG CTTTAAAAAC AGCATGTACC ATGTTGGAGA TTACGTCTAT 
GTGGAACCTG CAGAGGCCAA CCTACAACCA CATATTGTCT GTATTGAAAG ACTGTGGGAG 
GATTCAGCTG GTGAAAAATG GTTGTATGGC TGTTGGTTTT ACCGACCAAA TGAAACATTC 
CACCTGGCTA CACGAAAATT TCTAGAAAAA GAAGTTTTTA AGAGTGACTA TTACAACAAA 
GTTCCAGTTA GTAAAATTCT AGGCAAGTGT GTGGTCATGT TTGTCAAGGA ATACTTTAAG 
TTATGCCCAG AAAACTTCCG AGATGAGGAT GTTTTTGTCT GTGAATCACG GTATTCTGCC 
AAAACCAAAT CTTTTAAGAA AATTAAACTG TGGACCATGC CCATCAGCTC AGTTAGGTTT 
GTCCCTCGGG ATGTGCCTCT GCCTGTGGTT CGCGTGGCCT CTGTATTTGC AAATGCAGAT 
AAAGGTGATG ATGAGAAGAA TACAGACAAC TCAGAGGACA GTCGAGCTGA AGACAATTTT 
AACTTGGAAA AGGAAAAAGA AGATGTCCCT GTGGAAATGT CCAATGGTGA ACCAGGTTGC 
CACTACTTTG AGCAGCTCCA TTACAATGAC ATGTGGCTGA AGGTTGGCGA CTGTGTCTTC 
ATCAAGTCCC ATGGCCTGGT GCGTCCTCGT GTGGGCAGAA TTGAAAAAGT ATGGGTTCGA 
GATGGAGCTG CATATTTTTA TGGCCCCATC TTCATTCACC CAGAAGAAAC GGAGCATGAG 
CCCACAAAAA TGTTCTACAA AAAAGAAGTA TTTCTGAGTA ATCTGGAAGA AACCTGCCCC 
ATGACATGTA TTCTCGGAAA GTGTGCTGTG TTGTCATTCA AAGACTTCCT CTCCTGCAGG 
CCAACTGAAA TACCAGAAAA TGACATTCTG CTTTGTGAGA GCCGCTACAA TGAAAGTGAC 
AAGCAGATGA AGAAATTCAA AGGATTGAAG AGGTTTTCAC TCTCTGCTAA AGTGGTAGAT 
GATGAAATTT ACTACTTCAG AAAACCAATT ATTCCTCAGA AGGAGCCATC ACCTTTGTTG 
GAAAAGAAGA TCCAGTTGCT GGAAGCTAAA TTTGCCGAGT TAGAAGGTGG AGATGATGAT 
ATTGAAGAGA TGGGAGAAGA AGATAGTGAG GTCATTGAAC CTCCTTCTCT ACCTCAGCTT 
CAGACCCCCC TGGCCAGTGA GCTGGACCTC ATGCCCTACA CACCCCCACA GTCTACCCCA 
AAGTCTGCCA AAGGCAGTGC AAAGAAGGAA GGCTCCAAAC GGAAAATCAA CATGAGTGGC 
TACATCCTGT TCAGCAGTGA GATGAGGGCT GTGATTAAGG CCCAACACCC AGATTACTCT 
TTCGGGGAGC TCAGCCGCCT GGTGGGGACA GAATGGAGAA ATCTTGAGAC AGCCAAGAAA 
GCGGAATATG AAGAGCGGGC AGCTAAAGTT GCTGAGCAGC AGGAGAGAGA GCGAGCAGCA 
CAGCAACAGC AGCCGAGTGC TTCTCCCCGA GCAGGCACCC CTGTGGGGGC TCTCATGGGG 
GTGGTGCCAC CACCAACACC AATGGGGATG CTCAATCAGC AGTTGACACC TGTTGCAGGC 
ATGATGGGTG GCTATCCGCC AGGCCTTCCA CCTTTGCAGG GCCCAGTTGA TGGCCTTGTT 
AGCATGGGCA GCATGCAGCC ACTTCACCCT GGGGGGCCTC CACCCCACCA TCTTCCGCCA 
GGTGTGCCTG GCCTCCCGGG CATCCCACCA CCGGGTGTGA TGAACCAAGG AGTGGCCCCT 
ATGGTAGGGA CTCCAGCACC AGGTGGAAGT CCATATGGAC AACAGGTGGG AGTTTTGGGG 
CCTCCAGGGC AGCAGGCACC ACCTCCATAT CCCGGCCCAC ATCCACCTGG ACCCCCTGTC 
ATACAGCAGC CAACAACACC CATGTTTGTA GCTCCCCCAC CAAAGACCCA GCGGCTTCTT 
CACTCAGAGG CCTACCTGAA ATACATTGAA GGACTCAGTG CCGAGTCCAA CAGCATTAGC 
AAGTGGGATC AGACACTGGC AGCTCGAAGA CGTGACGTCC ATTTGTCAAA AGAACAGGAG 
AGCCGCCTAC CCTCTCACTG GCTGAAAAGC AAAGGGGCCC ACACCACCAT GGCAGATGCC 
CTCTGGCGCC TTCGAGATTT GATGCTGCGG GACACTCTCA ACATTCGCCA AGCATACAAC 
CTAGAAAACA TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PBRM1
Entrez Gene ID	100458266
Full Name	polybromo 1
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PBRM1 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

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