AFF1 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFF1
Entrez Gene ID	100460235
Full Name	AF4/FMR2 family member 1
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024245831.1	XP_024101599.1	AF4/FMR2 family member 1 isoform X1
XM_024245833.1	XP_024101601.1	AF4/FMR2 family member 1 isoform X2
XM_024245835.1	XP_024101603.1	AF4/FMR2 family member 1 isoform X4
XM_009240181.1	XP_009238456.1	LOW QUALITY PROTEIN: AF4/FMR2 family member 1
XM_024245834.1	XP_024101602.1	AF4/FMR2 family member 1 isoform X3
XM_024245832.1	XP_024101600.1	AF4/FMR2 family member 1 isoform X1
XM_024245836.1	XP_024101604.1	AF4/FMR2 family member 1 isoform X5

Pathways from BioSystems
Gene Ontology

The following AFF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSa180854	XM_024245832.1 Latest version!	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa180854	XM_024245831.1 Latest version!	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa180855	XM_024245833.1 Latest version!	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa180856	XM_024245834.1 Latest version!	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa180857	XM_024245835.1 Latest version!	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa180858	XM_024245836.1 Latest version!	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OSa35184	XM_009240181.1 Latest version!	Pongo abelii AF4/FMR2 family, member 1 (AFF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFF1 ( XM_024245831.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024101599.1 Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSa180854
Clone ID Related Accession (Same CDS sequence)	XM_024245831.1 , XM_024245832.1
Accession Version	XM_024245831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	AF4/FMR2 family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGCAGACCTA CTCCAATGAA 
GTCCATTGTG TTGAAGAGAT TCTGAAGGAA ATGACCCATT CATGGCCTCC TCCTTTGACA 
GCAATACATA CGCCTAGTAC AGCTGAGCCA TCCAAGTTTC CTTTCCCCAC AAAGGACTCT 
CAGCACGTCA GTTCTGTAAC CCAAAACCAA AAACAATATG ATACATCTTC AAAAACTCAC 
TCAAATTCTC AGCAAGGAAC GTCATCCATG CTCGAAGATG ACCTTCAGCT CAGTGACAGT 
GAGGACAGTG ACAGTGAACA AACCCCAGAG AAGCCTCCCT CCTCATCTGC ACCTCCAAGT 
GCTCCACAGT CCCTTCCAGA ACCAGTGGCA TCAGCACATT CCAGCAGTGC AGAGTCAGAA 
AGCACCAGTG ACTCAGACAG TTCCTCAGAC TCGGAGAGCG AGAGCAGTTC AAGTGACAGC 
GAAGAAAATG AGCCCCTAGA AACCCCGGCT CCGGAGCCTG AGCCTCCAAC AACAAACAAA 
TGGCAGCTGG ACAACTGGCT GACCAAAGTC AGCCAGCCAG CTGCGCCACT GGAGGGCCCC 
GGAAGCACAG AGCCCCCACG GCGGCACCCA GAGAGTAAGG GCAGCAGTGA CAGTGCCACG 
AGTCAGGAGC ATTCTGAATC CAAAGATCCT CCCCCTAAAA GCTCCAGCAA AGCCCTCCGG 
GCCCCACCCG AAGCCCCCCA CCCCGGAAAG AGGAGCTGTC AGAAGTCTCC GGCACAGCAG 
GAGCCCCCAC AGAGGCAAAC CGTTGGAACC AAACAACCCA AAAAACCTGT CAAGGCCTCT 
GCCCGGGCAG GTTCACGGGC CAGCCTGCAG GGGGAAAGGG AGCCAGGGCT TCTTCCCTAC 
GGCTCCCGAG ACCAGACTTC CAAAGACAAG CCCAAGGTGA AGACGAAAGG ACGGCCCCGG 
GCCGCAGCAA GCAACGAGCC CAAGCCAGCA GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC 
AAGAGCTCCC TCCCTGCTCC CTCTAAGGCT CCCTCAGGCC CAGAACCCAC GAAGGACAAT 
GTGGAGGACA GGAGCCCTGA GCACTTTGCT CTTGTTCCCC TGACTCAGAG CCAGGGCCCA 
CCCCACAGTG GCAGCGGCAG CGGCAGCAGG ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC 
CAGGAGGACA GCCGCAAAGA CAGACTCCCA TTGCCTTTGA GAGACACCAA GCTGCTCTCA 
CCACTCAGGG ACACTCCTCC CCCACAAAGC TTGATGGTGA AGATCACCCT AGACCTGCTC 
TCTCGGATAC CCCAGCCTCC CGGGAAGAGG AGCCGCCAGA GGAAAGCAGA AGATAAACAG 
CCACCCGCAG GGAAGAAGCA CAGCTCTGAG AAGAGGAGCT CAGACAGCTC AAGCAAGTTG 
GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA GACTGTGATA ACAAGAAAAT CAGACTGGAG 
AAGGAAATCA AATCACAGTC ATCTTCATCT TCATCCTCCC ACAAAGAATC TTCTAAAACA 
AAGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC 
TCTCAGAAGC CAGCCAAGCC TGCACATAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC 
CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC 
AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC 
GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG 
AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG 
ATGAAGCAGA AAGCAGAGTT AATGACGGAC AAGGTTGGAA AGGCTTTTAA GTACCTGGAA 
GCCGTCTTGT CCTTCATCGA GTGTGGAATT GCTACAGAGT CTGAAAGCCA GGCATCCAAG 
TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA 
TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCCGTTTT ATGCATGCGT 
TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT 
TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA 
TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC 
TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC 
AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC GTCCCATGTT 
CTTACTGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CAAGGAAGAA TAAAGAATTC 
TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG 
GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024101599.1
CDS	290..3946
Translation	MAFTERVNSSGNSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDAPENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPEREPSPLISSLPSPVPPLSPIHSNQQTLPRTQGSSKVHGSNNNSKGCCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPLEGPGSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKALRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRASLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKAPSGPEPTKDNVEDRSPEHFALVPLTQSQGPPHSGSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKRSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSSQSSKKEMLPPPPVSSSSQKPAKPAHKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDKVGKAFKYLEAVLSFIECGIATESESQASKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIARSTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Target ORF information:

RefSeq Version	XM_024245831.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024245831.1

ORF Insert Sequence:

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGCAGACCTA CTCCAATGAA 
GTCCATTGTG TTGAAGAGAT TCTGAAGGAA ATGACCCATT CATGGCCTCC TCCTTTGACA 
GCAATACATA CGCCTAGTAC AGCTGAGCCA TCCAAGTTTC CTTTCCCCAC AAAGGACTCT 
CAGCACGTCA GTTCTGTAAC CCAAAACCAA AAACAATATG ATACATCTTC AAAAACTCAC 
TCAAATTCTC AGCAAGGAAC GTCATCCATG CTCGAAGATG ACCTTCAGCT CAGTGACAGT 
GAGGACAGTG ACAGTGAACA AACCCCAGAG AAGCCTCCCT CCTCATCTGC ACCTCCAAGT 
GCTCCACAGT CCCTTCCAGA ACCAGTGGCA TCAGCACATT CCAGCAGTGC AGAGTCAGAA 
AGCACCAGTG ACTCAGACAG TTCCTCAGAC TCGGAGAGCG AGAGCAGTTC AAGTGACAGC 
GAAGAAAATG AGCCCCTAGA AACCCCGGCT CCGGAGCCTG AGCCTCCAAC AACAAACAAA 
TGGCAGCTGG ACAACTGGCT GACCAAAGTC AGCCAGCCAG CTGCGCCACT GGAGGGCCCC 
GGAAGCACAG AGCCCCCACG GCGGCACCCA GAGAGTAAGG GCAGCAGTGA CAGTGCCACG 
AGTCAGGAGC ATTCTGAATC CAAAGATCCT CCCCCTAAAA GCTCCAGCAA AGCCCTCCGG 
GCCCCACCCG AAGCCCCCCA CCCCGGAAAG AGGAGCTGTC AGAAGTCTCC GGCACAGCAG 
GAGCCCCCAC AGAGGCAAAC CGTTGGAACC AAACAACCCA AAAAACCTGT CAAGGCCTCT 
GCCCGGGCAG GTTCACGGGC CAGCCTGCAG GGGGAAAGGG AGCCAGGGCT TCTTCCCTAC 
GGCTCCCGAG ACCAGACTTC CAAAGACAAG CCCAAGGTGA AGACGAAAGG ACGGCCCCGG 
GCCGCAGCAA GCAACGAGCC CAAGCCAGCA GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC 
AAGAGCTCCC TCCCTGCTCC CTCTAAGGCT CCCTCAGGCC CAGAACCCAC GAAGGACAAT 
GTGGAGGACA GGAGCCCTGA GCACTTTGCT CTTGTTCCCC TGACTCAGAG CCAGGGCCCA 
CCCCACAGTG GCAGCGGCAG CGGCAGCAGG ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC 
CAGGAGGACA GCCGCAAAGA CAGACTCCCA TTGCCTTTGA GAGACACCAA GCTGCTCTCA 
CCACTCAGGG ACACTCCTCC CCCACAAAGC TTGATGGTGA AGATCACCCT AGACCTGCTC 
TCTCGGATAC CCCAGCCTCC CGGGAAGAGG AGCCGCCAGA GGAAAGCAGA AGATAAACAG 
CCACCCGCAG GGAAGAAGCA CAGCTCTGAG AAGAGGAGCT CAGACAGCTC AAGCAAGTTG 
GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA GACTGTGATA ACAAGAAAAT CAGACTGGAG 
AAGGAAATCA AATCACAGTC ATCTTCATCT TCATCCTCCC ACAAAGAATC TTCTAAAACA 
AAGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC 
TCTCAGAAGC CAGCCAAGCC TGCACATAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC 
CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC 
AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC 
GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG 
AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG 
ATGAAGCAGA AAGCAGAGTT AATGACGGAC AAGGTTGGAA AGGCTTTTAA GTACCTGGAA 
GCCGTCTTGT CCTTCATCGA GTGTGGAATT GCTACAGAGT CTGAAAGCCA GGCATCCAAG 
TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA 
TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCCGTTTT ATGCATGCGT 
TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT 
TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA 
TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC 
TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC 
AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC GTCCCATGTT 
CTTACTGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CAAGGAAGAA TAAAGAATTC 
TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG 
GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_024245832.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024101600.1 Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSa180854
Clone ID Related Accession (Same CDS sequence)	XM_024245831.1 , XM_024245832.1
Accession Version	XM_024245832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	AF4/FMR2 family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGCAGACCTA CTCCAATGAA 
GTCCATTGTG TTGAAGAGAT TCTGAAGGAA ATGACCCATT CATGGCCTCC TCCTTTGACA 
GCAATACATA CGCCTAGTAC AGCTGAGCCA TCCAAGTTTC CTTTCCCCAC AAAGGACTCT 
CAGCACGTCA GTTCTGTAAC CCAAAACCAA AAACAATATG ATACATCTTC AAAAACTCAC 
TCAAATTCTC AGCAAGGAAC GTCATCCATG CTCGAAGATG ACCTTCAGCT CAGTGACAGT 
GAGGACAGTG ACAGTGAACA AACCCCAGAG AAGCCTCCCT CCTCATCTGC ACCTCCAAGT 
GCTCCACAGT CCCTTCCAGA ACCAGTGGCA TCAGCACATT CCAGCAGTGC AGAGTCAGAA 
AGCACCAGTG ACTCAGACAG TTCCTCAGAC TCGGAGAGCG AGAGCAGTTC AAGTGACAGC 
GAAGAAAATG AGCCCCTAGA AACCCCGGCT CCGGAGCCTG AGCCTCCAAC AACAAACAAA 
TGGCAGCTGG ACAACTGGCT GACCAAAGTC AGCCAGCCAG CTGCGCCACT GGAGGGCCCC 
GGAAGCACAG AGCCCCCACG GCGGCACCCA GAGAGTAAGG GCAGCAGTGA CAGTGCCACG 
AGTCAGGAGC ATTCTGAATC CAAAGATCCT CCCCCTAAAA GCTCCAGCAA AGCCCTCCGG 
GCCCCACCCG AAGCCCCCCA CCCCGGAAAG AGGAGCTGTC AGAAGTCTCC GGCACAGCAG 
GAGCCCCCAC AGAGGCAAAC CGTTGGAACC AAACAACCCA AAAAACCTGT CAAGGCCTCT 
GCCCGGGCAG GTTCACGGGC CAGCCTGCAG GGGGAAAGGG AGCCAGGGCT TCTTCCCTAC 
GGCTCCCGAG ACCAGACTTC CAAAGACAAG CCCAAGGTGA AGACGAAAGG ACGGCCCCGG 
GCCGCAGCAA GCAACGAGCC CAAGCCAGCA GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC 
AAGAGCTCCC TCCCTGCTCC CTCTAAGGCT CCCTCAGGCC CAGAACCCAC GAAGGACAAT 
GTGGAGGACA GGAGCCCTGA GCACTTTGCT CTTGTTCCCC TGACTCAGAG CCAGGGCCCA 
CCCCACAGTG GCAGCGGCAG CGGCAGCAGG ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC 
CAGGAGGACA GCCGCAAAGA CAGACTCCCA TTGCCTTTGA GAGACACCAA GCTGCTCTCA 
CCACTCAGGG ACACTCCTCC CCCACAAAGC TTGATGGTGA AGATCACCCT AGACCTGCTC 
TCTCGGATAC CCCAGCCTCC CGGGAAGAGG AGCCGCCAGA GGAAAGCAGA AGATAAACAG 
CCACCCGCAG GGAAGAAGCA CAGCTCTGAG AAGAGGAGCT CAGACAGCTC AAGCAAGTTG 
GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA GACTGTGATA ACAAGAAAAT CAGACTGGAG 
AAGGAAATCA AATCACAGTC ATCTTCATCT TCATCCTCCC ACAAAGAATC TTCTAAAACA 
AAGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC 
TCTCAGAAGC CAGCCAAGCC TGCACATAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC 
CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC 
AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC 
GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG 
AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG 
ATGAAGCAGA AAGCAGAGTT AATGACGGAC AAGGTTGGAA AGGCTTTTAA GTACCTGGAA 
GCCGTCTTGT CCTTCATCGA GTGTGGAATT GCTACAGAGT CTGAAAGCCA GGCATCCAAG 
TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA 
TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCCGTTTT ATGCATGCGT 
TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT 
TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA 
TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC 
TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC 
AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC GTCCCATGTT 
CTTACTGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CAAGGAAGAA TAAAGAATTC 
TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG 
GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024101600.1
CDS	260..3916
Translation	MAFTERVNSSGNSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDAPENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPEREPSPLISSLPSPVPPLSPIHSNQQTLPRTQGSSKVHGSNNNSKGCCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPLEGPGSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKALRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRASLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKAPSGPEPTKDNVEDRSPEHFALVPLTQSQGPPHSGSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKRSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSSQSSKKEMLPPPPVSSSSQKPAKPAHKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDKVGKAFKYLEAVLSFIECGIATESESQASKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIARSTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Target ORF information:

RefSeq Version	XM_024245832.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024245832.1

ORF Insert Sequence:

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGCAGACCTA CTCCAATGAA 
GTCCATTGTG TTGAAGAGAT TCTGAAGGAA ATGACCCATT CATGGCCTCC TCCTTTGACA 
GCAATACATA CGCCTAGTAC AGCTGAGCCA TCCAAGTTTC CTTTCCCCAC AAAGGACTCT 
CAGCACGTCA GTTCTGTAAC CCAAAACCAA AAACAATATG ATACATCTTC AAAAACTCAC 
TCAAATTCTC AGCAAGGAAC GTCATCCATG CTCGAAGATG ACCTTCAGCT CAGTGACAGT 
GAGGACAGTG ACAGTGAACA AACCCCAGAG AAGCCTCCCT CCTCATCTGC ACCTCCAAGT 
GCTCCACAGT CCCTTCCAGA ACCAGTGGCA TCAGCACATT CCAGCAGTGC AGAGTCAGAA 
AGCACCAGTG ACTCAGACAG TTCCTCAGAC TCGGAGAGCG AGAGCAGTTC AAGTGACAGC 
GAAGAAAATG AGCCCCTAGA AACCCCGGCT CCGGAGCCTG AGCCTCCAAC AACAAACAAA 
TGGCAGCTGG ACAACTGGCT GACCAAAGTC AGCCAGCCAG CTGCGCCACT GGAGGGCCCC 
GGAAGCACAG AGCCCCCACG GCGGCACCCA GAGAGTAAGG GCAGCAGTGA CAGTGCCACG 
AGTCAGGAGC ATTCTGAATC CAAAGATCCT CCCCCTAAAA GCTCCAGCAA AGCCCTCCGG 
GCCCCACCCG AAGCCCCCCA CCCCGGAAAG AGGAGCTGTC AGAAGTCTCC GGCACAGCAG 
GAGCCCCCAC AGAGGCAAAC CGTTGGAACC AAACAACCCA AAAAACCTGT CAAGGCCTCT 
GCCCGGGCAG GTTCACGGGC CAGCCTGCAG GGGGAAAGGG AGCCAGGGCT TCTTCCCTAC 
GGCTCCCGAG ACCAGACTTC CAAAGACAAG CCCAAGGTGA AGACGAAAGG ACGGCCCCGG 
GCCGCAGCAA GCAACGAGCC CAAGCCAGCA GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC 
AAGAGCTCCC TCCCTGCTCC CTCTAAGGCT CCCTCAGGCC CAGAACCCAC GAAGGACAAT 
GTGGAGGACA GGAGCCCTGA GCACTTTGCT CTTGTTCCCC TGACTCAGAG CCAGGGCCCA 
CCCCACAGTG GCAGCGGCAG CGGCAGCAGG ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC 
CAGGAGGACA GCCGCAAAGA CAGACTCCCA TTGCCTTTGA GAGACACCAA GCTGCTCTCA 
CCACTCAGGG ACACTCCTCC CCCACAAAGC TTGATGGTGA AGATCACCCT AGACCTGCTC 
TCTCGGATAC CCCAGCCTCC CGGGAAGAGG AGCCGCCAGA GGAAAGCAGA AGATAAACAG 
CCACCCGCAG GGAAGAAGCA CAGCTCTGAG AAGAGGAGCT CAGACAGCTC AAGCAAGTTG 
GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA GACTGTGATA ACAAGAAAAT CAGACTGGAG 
AAGGAAATCA AATCACAGTC ATCTTCATCT TCATCCTCCC ACAAAGAATC TTCTAAAACA 
AAGCCCTCCT CACAGTCCTC AAAGAAGGAA ATGCTCCCCC CGCCACCCGT GTCCTCGTCC 
TCTCAGAAGC CAGCCAAGCC TGCACATAAG AGGTCAAGGC GGGAAGCAGA CACCTGTGGC 
CAGGACCCTC CCAAAAGTGC CAGCAGTACC AAGAGCAACC ACAAAGACTC TTCCATTCCC 
AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC AGAAGCTCCT CGGAGCACAA GGGTTCTTCC 
GGAGATACTG CAAATCCTTT TCCAGTGCCT TCTTTGCCAA ATGGTAACTC TAAACCAGGG 
AAGCCTCAAG TGAAGTTTGA CAAACAACAA GCAGACCTTC ACATGAGGGA GGCAAAAAAG 
ATGAAGCAGA AAGCAGAGTT AATGACGGAC AAGGTTGGAA AGGCTTTTAA GTACCTGGAA 
GCCGTCTTGT CCTTCATCGA GTGTGGAATT GCTACAGAGT CTGAAAGCCA GGCATCCAAG 
TCAGCTTACT CTGTCTACTC AGAAACTGTA GATCTCATTA AATTCATAAT GTCATTAAAA 
TCCTTCTCAG ATGCCACAGC GCCAACACAA GAGAAAATAT TTGCCGTTTT ATGCATGCGT 
TGCCAGTCCA TTTTGAACAT GGCGATGTTT CGTTGTAAAA AAGACATAGC AATAAAGTAT 
TCTCGTACTC TTAATAAACA CTTCGAGAGT TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA 
TGCATTGCAA GAAGCACAGG CACACCATCC CCTCTTTCCC CAATGCCTTC TCCTGCCAGC 
TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT GTGGGGAGCA GTGGGGTGGC TGCCACTATC 
AGCACCCCAG TCACCATCCA GAATATGACA TCTTCCTATG TCACCATCAC GTCCCATGTT 
CTTACTGCCT TTGACCTTTG GGAACAGGCC GAGGCCCTCA CAAGGAAGAA TAAAGAATTC 
TTTGCTCGGC TCAGCACAAA TGTGTGCACC TTGGCCCTCA ACAGCAGTTT GGTGGACCTG 
GTGCACTATA CACGACAGGG TTTTCAGCAG CTACAAGAAT TAACCAAAAC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_024245833.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024101601.1 Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

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CloneID	OSa180855
Clone ID Related Accession (Same CDS sequence)	XM_024245833.1
Accession Version	XM_024245833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	AF4/FMR2 family member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGACCTACTC CAATGAAGTC 
CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCTCCTCC TTTGACAGCA 
ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCCACAAA GGACTCTCAG 
CACGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA 
AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGATGACC TTCAGCTCAG TGACAGTGAG 
GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT 
CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC 
ACCAGTGACT CAGACAGTTC CTCAGACTCG GAGAGCGAGA GCAGTTCAAG TGACAGCGAA 
GAAAATGAGC CCCTAGAAAC CCCGGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG 
CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACTGGA GGGCCCCGGA 
AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGTGACAG TGCCACGAGT 
CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCTCCGGGCC 
CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG 
CCCCCACAGA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC 
CGGGCAGGTT CACGGGCCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTACGGC 
TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC 
GCAGCAAGCA ACGAGCCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG 
AGCTCCCTCC CTGCTCCCTC TAAGGCTCCC TCAGGCCCAG AACCCACGAA GGACAATGTG 
GAGGACAGGA GCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTCAGAGCCA GGGCCCACCC 
CACAGTGGCA GCGGCAGCGG CAGCAGGACT AGTGGCTGCC GCCAAGCCGT GGTGGTCCAG 
GAGGACAGCC GCAAAGACAG ACTCCCATTG CCTTTGAGAG ACACCAAGCT GCTCTCACCA 
CTCAGGGACA CTCCTCCCCC ACAAAGCTTG ATGGTGAAGA TCACCCTAGA CCTGCTCTCT 
CGGATACCCC AGCCTCCCGG GAAGAGGAGC CGCCAGAGGA AAGCAGAAGA TAAACAGCCA 
CCCGCAGGGA AGAAGCACAG CTCTGAGAAG AGGAGCTCAG ACAGCTCAAG CAAGTTGGCC 
AAAAAGAGAA AGGGTGAAGC AGAAAGAGAC TGTGATAACA AGAAAATCAG ACTGGAGAAG 
GAAATCAAAT CACAGTCATC TTCATCTTCA TCCTCCCACA AAGAATCTTC TAAAACAAAG 
CCCTCCTCAC AGTCCTCAAA GAAGGAAATG CTCCCCCCGC CACCCGTGTC CTCGTCCTCT 
CAGAAGCCAG CCAAGCCTGC ACATAAGAGG TCAAGGCGGG AAGCAGACAC CTGTGGCCAG 
GACCCTCCCA AAAGTGCCAG CAGTACCAAG AGCAACCACA AAGACTCTTC CATTCCCAAG 
CAGAGAAGAG TAGAGGGGAA GGGCTCCAGA AGCTCCTCGG AGCACAAGGG TTCTTCCGGA 
GATACTGCAA ATCCTTTTCC AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG 
CCTCAAGTGA AGTTTGACAA ACAACAAGCA GACCTTCACA TGAGGGAGGC AAAAAAGATG 
AAGCAGAAAG CAGAGTTAAT GACGGACAAG GTTGGAAAGG CTTTTAAGTA CCTGGAAGCC 
GTCTTGTCCT TCATCGAGTG TGGAATTGCT ACAGAGTCTG AAAGCCAGGC ATCCAAGTCA 
GCTTACTCTG TCTACTCAGA AACTGTAGAT CTCATTAAAT TCATAATGTC ATTAAAATCC 
TTCTCAGATG CCACAGCGCC AACACAAGAG AAAATATTTG CCGTTTTATG CATGCGTTGC 
CAGTCCATTT TGAACATGGC GATGTTTCGT TGTAAAAAAG ACATAGCAAT AAAGTATTCT 
CGTACTCTTA ATAAACACTT CGAGAGTTCT TCCAAAGTCG CCCAGGCACC TTCTCCATGC 
ATTGCAAGAA GCACAGGCAC ACCATCCCCT CTTTCCCCAA TGCCTTCTCC TGCCAGCTCC 
GTAGGGTCCC AGTCAAGTGC TGGCAGTGTG GGGAGCAGTG GGGTGGCTGC CACTATCAGC 
ACCCCAGTCA CCATCCAGAA TATGACATCT TCCTATGTCA CCATCACGTC CCATGTTCTT 
ACTGCCTTTG ACCTTTGGGA ACAGGCCGAG GCCCTCACAA GGAAGAATAA AGAATTCTTT 
GCTCGGCTCA GCACAAATGT GTGCACCTTG GCCCTCAACA GCAGTTTGGT GGACCTGGTG 
CACTATACAC GACAGGGTTT TCAGCAGCTA CAAGAATTAA CCAAAACACC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024101601.1
CDS	290..3943
Translation	MAFTERVNSSGNSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDAPENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPEREPSPLISSLPSPVPPLSPIHSNQQTLPRTQGSSKVHGSNNNSKGCCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVETYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPLEGPGSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKALRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRASLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKAPSGPEPTKDNVEDRSPEHFALVPLTQSQGPPHSGSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKRSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSSQSSKKEMLPPPPVSSSSQKPAKPAHKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDKVGKAFKYLEAVLSFIECGIATESESQASKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIARSTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Target ORF information:

RefSeq Version	XM_024245833.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024245833.1

ORF Insert Sequence:

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGACCTACTC CAATGAAGTC 
CATTGTGTTG AAGAGATTCT GAAGGAAATG ACCCATTCAT GGCCTCCTCC TTTGACAGCA 
ATACATACGC CTAGTACAGC TGAGCCATCC AAGTTTCCTT TCCCCACAAA GGACTCTCAG 
CACGTCAGTT CTGTAACCCA AAACCAAAAA CAATATGATA CATCTTCAAA AACTCACTCA 
AATTCTCAGC AAGGAACGTC ATCCATGCTC GAAGATGACC TTCAGCTCAG TGACAGTGAG 
GACAGTGACA GTGAACAAAC CCCAGAGAAG CCTCCCTCCT CATCTGCACC TCCAAGTGCT 
CCACAGTCCC TTCCAGAACC AGTGGCATCA GCACATTCCA GCAGTGCAGA GTCAGAAAGC 
ACCAGTGACT CAGACAGTTC CTCAGACTCG GAGAGCGAGA GCAGTTCAAG TGACAGCGAA 
GAAAATGAGC CCCTAGAAAC CCCGGCTCCG GAGCCTGAGC CTCCAACAAC AAACAAATGG 
CAGCTGGACA ACTGGCTGAC CAAAGTCAGC CAGCCAGCTG CGCCACTGGA GGGCCCCGGA 
AGCACAGAGC CCCCACGGCG GCACCCAGAG AGTAAGGGCA GCAGTGACAG TGCCACGAGT 
CAGGAGCATT CTGAATCCAA AGATCCTCCC CCTAAAAGCT CCAGCAAAGC CCTCCGGGCC 
CCACCCGAAG CCCCCCACCC CGGAAAGAGG AGCTGTCAGA AGTCTCCGGC ACAGCAGGAG 
CCCCCACAGA GGCAAACCGT TGGAACCAAA CAACCCAAAA AACCTGTCAA GGCCTCTGCC 
CGGGCAGGTT CACGGGCCAG CCTGCAGGGG GAAAGGGAGC CAGGGCTTCT TCCCTACGGC 
TCCCGAGACC AGACTTCCAA AGACAAGCCC AAGGTGAAGA CGAAAGGACG GCCCCGGGCC 
GCAGCAAGCA ACGAGCCCAA GCCAGCAGTG CCCCCCTCCA GTGAGAAGAA GAAGCACAAG 
AGCTCCCTCC CTGCTCCCTC TAAGGCTCCC TCAGGCCCAG AACCCACGAA GGACAATGTG 
GAGGACAGGA GCCCTGAGCA CTTTGCTCTT GTTCCCCTGA CTCAGAGCCA GGGCCCACCC 
CACAGTGGCA GCGGCAGCGG CAGCAGGACT AGTGGCTGCC GCCAAGCCGT GGTGGTCCAG 
GAGGACAGCC GCAAAGACAG ACTCCCATTG CCTTTGAGAG ACACCAAGCT GCTCTCACCA 
CTCAGGGACA CTCCTCCCCC ACAAAGCTTG ATGGTGAAGA TCACCCTAGA CCTGCTCTCT 
CGGATACCCC AGCCTCCCGG GAAGAGGAGC CGCCAGAGGA AAGCAGAAGA TAAACAGCCA 
CCCGCAGGGA AGAAGCACAG CTCTGAGAAG AGGAGCTCAG ACAGCTCAAG CAAGTTGGCC 
AAAAAGAGAA AGGGTGAAGC AGAAAGAGAC TGTGATAACA AGAAAATCAG ACTGGAGAAG 
GAAATCAAAT CACAGTCATC TTCATCTTCA TCCTCCCACA AAGAATCTTC TAAAACAAAG 
CCCTCCTCAC AGTCCTCAAA GAAGGAAATG CTCCCCCCGC CACCCGTGTC CTCGTCCTCT 
CAGAAGCCAG CCAAGCCTGC ACATAAGAGG TCAAGGCGGG AAGCAGACAC CTGTGGCCAG 
GACCCTCCCA AAAGTGCCAG CAGTACCAAG AGCAACCACA AAGACTCTTC CATTCCCAAG 
CAGAGAAGAG TAGAGGGGAA GGGCTCCAGA AGCTCCTCGG AGCACAAGGG TTCTTCCGGA 
GATACTGCAA ATCCTTTTCC AGTGCCTTCT TTGCCAAATG GTAACTCTAA ACCAGGGAAG 
CCTCAAGTGA AGTTTGACAA ACAACAAGCA GACCTTCACA TGAGGGAGGC AAAAAAGATG 
AAGCAGAAAG CAGAGTTAAT GACGGACAAG GTTGGAAAGG CTTTTAAGTA CCTGGAAGCC 
GTCTTGTCCT TCATCGAGTG TGGAATTGCT ACAGAGTCTG AAAGCCAGGC ATCCAAGTCA 
GCTTACTCTG TCTACTCAGA AACTGTAGAT CTCATTAAAT TCATAATGTC ATTAAAATCC 
TTCTCAGATG CCACAGCGCC AACACAAGAG AAAATATTTG CCGTTTTATG CATGCGTTGC 
CAGTCCATTT TGAACATGGC GATGTTTCGT TGTAAAAAAG ACATAGCAAT AAAGTATTCT 
CGTACTCTTA ATAAACACTT CGAGAGTTCT TCCAAAGTCG CCCAGGCACC TTCTCCATGC 
ATTGCAAGAA GCACAGGCAC ACCATCCCCT CTTTCCCCAA TGCCTTCTCC TGCCAGCTCC 
GTAGGGTCCC AGTCAAGTGC TGGCAGTGTG GGGAGCAGTG GGGTGGCTGC CACTATCAGC 
ACCCCAGTCA CCATCCAGAA TATGACATCT TCCTATGTCA CCATCACGTC CCATGTTCTT 
ACTGCCTTTG ACCTTTGGGA ACAGGCCGAG GCCCTCACAA GGAAGAATAA AGAATTCTTT 
GCTCGGCTCA GCACAAATGT GTGCACCTTG GCCCTCAACA GCAGTTTGGT GGACCTGGTG 
CACTATACAC GACAGGGTTT TCAGCAGCTA CAAGAATTAA CCAAAACACC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_024245834.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024101602.1 Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OSa180856
Clone ID Related Accession (Same CDS sequence)	XM_024245834.1
Accession Version	XM_024245834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	AF4/FMR2 family member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCCC AGTCAAGTTT GTACAATGAC GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
GAAAGACGCA ACCAGGAAGC CCACCAAGAG AAAGAGGCAT TTCCTGAAAA GATTCCCCTT 
TTTGGAGAGC CCTACAAGAC AGCAAAAGGT GATGAGCTGT CTAGTCGAAT ACAGAACATG 
TTGGGAAACT ACGAAGAAGT GAAGGAGTTC CTTAGTACTA AGTCTCACAC TCATCGCTTG 
GATGCTCCTG AAAATAGGTT GGGAAAGCCA AAATATCCTT TAATTCCTGA CAAAGGGAGC 
AGCATTCCAT CCAGCTCCTT CCACACTAGT GTCCACCACC AGTCCATTCA CACTCCTGCG 
TCTGGACCAC TTTCTGTTGG CAACATTAGC CACAATCCAA AGATGGCGCA GCCAAGAACT 
GAACCAATGC CAAGTCTCCA TGCCAAAAGC TGTGGCCCAC CGGACAGCCA GCACCTGACC 
CAGGATCGCC TTGGTCAGGA GGGGTTCGGC TCTAGTCATC ACAAGAAAGG TGACCGAAGA 
GCTGACGGAG ACCACTGTGC TTCGGTGACA GACTCGGCTC CAGAGAGGGA GCCTTCTCCC 
TTAATCTCCT CTTTGCCTTC CCCAGTTCCC CCTTTGTCAC CTATACATTC CAACCAGCAA 
ACTCTTCCCC GGACGCAAGG AAGCAGCAAG GTTCATGGCA GCAACAATAA CAGTAAAGGC 
TGTTGCCCAG CCAAATCTCC CAAGGACCTA GCAGTGAAAG TCCATGATAA AGAGACCCCT 
CAAGACAGTT TGGTGGCCCC TGCCCAGCCG CCTTCTCAGA CATTTCCACC TCCCTCCCTC 
CCCTCAAAAA GTGTTGCAAT GCAGCAGAAG CCCACGGCTT ATGTCCGGCC CATGGATGGT 
CAAGATCAGG CCCCTAGTGA ATCCCCTGAA CTGAAACCAC TGCCGGAGGA CTATCGACAG 
CAGACCTTTG AAAAAACAGA CTTGAAAGTG CCTGCCAAAG CCAAGCTCAC CAAACTGAAG 
ATGCCTTCTC AGTCAGTTGA GCAGACCTAC TCCAATGAAG TCCATTGTGT TGAAGAGATT 
CTGAAGGAAA TGACCCATTC ATGGCCTCCT CCTTTGACAG CAATACATAC GCCTAGTACA 
GCTGAGCCAT CCAAGTTTCC TTTCCCCACA AAGGACTCTC AGCACGTCAG TTCTGTAACC 
CAAAACCAAA AACAATATGA TACATCTTCA AAAACTCACT CAAATTCTCA GCAAGGAACG 
TCATCCATGC TCGAAGATGA CCTTCAGCTC AGTGACAGTG AGGACAGTGA CAGTGAACAA 
ACCCCAGAGA AGCCTCCCTC CTCATCTGCA CCTCCAAGTG CTCCACAGTC CCTTCCAGAA 
CCAGTGGCAT CAGCACATTC CAGCAGTGCA GAGTCAGAAA GCACCAGTGA CTCAGACAGT 
TCCTCAGACT CGGAGAGCGA GAGCAGTTCA AGTGACAGCG AAGAAAATGA GCCCCTAGAA 
ACCCCGGCTC CGGAGCCTGA GCCTCCAACA ACAAACAAAT GGCAGCTGGA CAACTGGCTG 
ACCAAAGTCA GCCAGCCAGC TGCGCCACTG GAGGGCCCCG GAAGCACAGA GCCCCCACGG 
CGGCACCCAG AGAGTAAGGG CAGCAGTGAC AGTGCCACGA GTCAGGAGCA TTCTGAATCC 
AAAGATCCTC CCCCTAAAAG CTCCAGCAAA GCCCTCCGGG CCCCACCCGA AGCCCCCCAC 
CCCGGAAAGA GGAGCTGTCA GAAGTCTCCG GCACAGCAGG AGCCCCCACA GAGGCAAACC 
GTTGGAACCA AACAACCCAA AAAACCTGTC AAGGCCTCTG CCCGGGCAGG TTCACGGGCC 
AGCCTGCAGG GGGAAAGGGA GCCAGGGCTT CTTCCCTACG GCTCCCGAGA CCAGACTTCC 
AAAGACAAGC CCAAGGTGAA GACGAAAGGA CGGCCCCGGG CCGCAGCAAG CAACGAGCCC 
AAGCCAGCAG TGCCCCCCTC CAGTGAGAAG AAGAAGCACA AGAGCTCCCT CCCTGCTCCC 
TCTAAGGCTC CCTCAGGCCC AGAACCCACG AAGGACAATG TGGAGGACAG GAGCCCTGAG 
CACTTTGCTC TTGTTCCCCT GACTCAGAGC CAGGGCCCAC CCCACAGTGG CAGCGGCAGC 
GGCAGCAGGA CTAGTGGCTG CCGCCAAGCC GTGGTGGTCC AGGAGGACAG CCGCAAAGAC 
AGACTCCCAT TGCCTTTGAG AGACACCAAG CTGCTCTCAC CACTCAGGGA CACTCCTCCC 
CCACAAAGCT TGATGGTGAA GATCACCCTA GACCTGCTCT CTCGGATACC CCAGCCTCCC 
GGGAAGAGGA GCCGCCAGAG GAAAGCAGAA GATAAACAGC CACCCGCAGG GAAGAAGCAC 
AGCTCTGAGA AGAGGAGCTC AGACAGCTCA AGCAAGTTGG CCAAAAAGAG AAAGGGTGAA 
GCAGAAAGAG ACTGTGATAA CAAGAAAATC AGACTGGAGA AGGAAATCAA ATCACAGTCA 
TCTTCATCTT CATCCTCCCA CAAAGAATCT TCTAAAACAA AGCCCTCCTC ACAGTCCTCA 
AAGAAGGAAA TGCTCCCCCC GCCACCCGTG TCCTCGTCCT CTCAGAAGCC AGCCAAGCCT 
GCACATAAGA GGTCAAGGCG GGAAGCAGAC ACCTGTGGCC AGGACCCTCC CAAAAGTGCC 
AGCAGTACCA AGAGCAACCA CAAAGACTCT TCCATTCCCA AGCAGAGAAG AGTAGAGGGG 
AAGGGCTCCA GAAGCTCCTC GGAGCACAAG GGTTCTTCCG GAGATACTGC AAATCCTTTT 
CCAGTGCCTT CTTTGCCAAA TGGTAACTCT AAACCAGGGA AGCCTCAAGT GAAGTTTGAC 
AAACAACAAG CAGACCTTCA CATGAGGGAG GCAAAAAAGA TGAAGCAGAA AGCAGAGTTA 
ATGACGGACA AGGTTGGAAA GGCTTTTAAG TACCTGGAAG CCGTCTTGTC CTTCATCGAG 
TGTGGAATTG CTACAGAGTC TGAAAGCCAG GCATCCAAGT CAGCTTACTC TGTCTACTCA 
GAAACTGTAG ATCTCATTAA ATTCATAATG TCATTAAAAT CCTTCTCAGA TGCCACAGCG 
CCAACACAAG AGAAAATATT TGCCGTTTTA TGCATGCGTT GCCAGTCCAT TTTGAACATG 
GCGATGTTTC GTTGTAAAAA AGACATAGCA ATAAAGTATT CTCGTACTCT TAATAAACAC 
TTCGAGAGTT CTTCCAAAGT CGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAGCACAGGC 
ACACCATCCC CTCTTTCCCC AATGCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCAAGT 
GCTGGCAGTG TGGGGAGCAG TGGGGTGGCT GCCACTATCA GCACCCCAGT CACCATCCAG 
AATATGACAT CTTCCTATGT CACCATCACG TCCCATGTTC TTACTGCCTT TGACCTTTGG 
GAACAGGCCG AGGCCCTCAC AAGGAAGAAT AAAGAATTCT TTGCTCGGCT CAGCACAAAT 
GTGTGCACCT TGGCCCTCAA CAGCAGTTTG GTGGACCTGG TGCACTATAC ACGACAGGGT 
TTTCAGCAGC TACAAGAATT AACCAAAACA CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024101602.1
CDS	406..4041
Translation	MAAQSSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDAPENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPEREPSPLISSLPSPVPPLSPIHSNQQTLPRTQGSSKVHGSNNNSKGCCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPLEGPGSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKALRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRASLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKAPSGPEPTKDNVEDRSPEHFALVPLTQSQGPPHSGSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKRSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSSQSSKKEMLPPPPVSSSSQKPAKPAHKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDKVGKAFKYLEAVLSFIECGIATESESQASKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIARSTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Target ORF information:

RefSeq Version	XM_024245834.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024245834.1

ORF Insert Sequence:

ATGGCAGCCC AGTCAAGTTT GTACAATGAC GACAGAAACC TGCTTCGAAT TAGAGAGAAG 
GAAAGACGCA ACCAGGAAGC CCACCAAGAG AAAGAGGCAT TTCCTGAAAA GATTCCCCTT 
TTTGGAGAGC CCTACAAGAC AGCAAAAGGT GATGAGCTGT CTAGTCGAAT ACAGAACATG 
TTGGGAAACT ACGAAGAAGT GAAGGAGTTC CTTAGTACTA AGTCTCACAC TCATCGCTTG 
GATGCTCCTG AAAATAGGTT GGGAAAGCCA AAATATCCTT TAATTCCTGA CAAAGGGAGC 
AGCATTCCAT CCAGCTCCTT CCACACTAGT GTCCACCACC AGTCCATTCA CACTCCTGCG 
TCTGGACCAC TTTCTGTTGG CAACATTAGC CACAATCCAA AGATGGCGCA GCCAAGAACT 
GAACCAATGC CAAGTCTCCA TGCCAAAAGC TGTGGCCCAC CGGACAGCCA GCACCTGACC 
CAGGATCGCC TTGGTCAGGA GGGGTTCGGC TCTAGTCATC ACAAGAAAGG TGACCGAAGA 
GCTGACGGAG ACCACTGTGC TTCGGTGACA GACTCGGCTC CAGAGAGGGA GCCTTCTCCC 
TTAATCTCCT CTTTGCCTTC CCCAGTTCCC CCTTTGTCAC CTATACATTC CAACCAGCAA 
ACTCTTCCCC GGACGCAAGG AAGCAGCAAG GTTCATGGCA GCAACAATAA CAGTAAAGGC 
TGTTGCCCAG CCAAATCTCC CAAGGACCTA GCAGTGAAAG TCCATGATAA AGAGACCCCT 
CAAGACAGTT TGGTGGCCCC TGCCCAGCCG CCTTCTCAGA CATTTCCACC TCCCTCCCTC 
CCCTCAAAAA GTGTTGCAAT GCAGCAGAAG CCCACGGCTT ATGTCCGGCC CATGGATGGT 
CAAGATCAGG CCCCTAGTGA ATCCCCTGAA CTGAAACCAC TGCCGGAGGA CTATCGACAG 
CAGACCTTTG AAAAAACAGA CTTGAAAGTG CCTGCCAAAG CCAAGCTCAC CAAACTGAAG 
ATGCCTTCTC AGTCAGTTGA GCAGACCTAC TCCAATGAAG TCCATTGTGT TGAAGAGATT 
CTGAAGGAAA TGACCCATTC ATGGCCTCCT CCTTTGACAG CAATACATAC GCCTAGTACA 
GCTGAGCCAT CCAAGTTTCC TTTCCCCACA AAGGACTCTC AGCACGTCAG TTCTGTAACC 
CAAAACCAAA AACAATATGA TACATCTTCA AAAACTCACT CAAATTCTCA GCAAGGAACG 
TCATCCATGC TCGAAGATGA CCTTCAGCTC AGTGACAGTG AGGACAGTGA CAGTGAACAA 
ACCCCAGAGA AGCCTCCCTC CTCATCTGCA CCTCCAAGTG CTCCACAGTC CCTTCCAGAA 
CCAGTGGCAT CAGCACATTC CAGCAGTGCA GAGTCAGAAA GCACCAGTGA CTCAGACAGT 
TCCTCAGACT CGGAGAGCGA GAGCAGTTCA AGTGACAGCG AAGAAAATGA GCCCCTAGAA 
ACCCCGGCTC CGGAGCCTGA GCCTCCAACA ACAAACAAAT GGCAGCTGGA CAACTGGCTG 
ACCAAAGTCA GCCAGCCAGC TGCGCCACTG GAGGGCCCCG GAAGCACAGA GCCCCCACGG 
CGGCACCCAG AGAGTAAGGG CAGCAGTGAC AGTGCCACGA GTCAGGAGCA TTCTGAATCC 
AAAGATCCTC CCCCTAAAAG CTCCAGCAAA GCCCTCCGGG CCCCACCCGA AGCCCCCCAC 
CCCGGAAAGA GGAGCTGTCA GAAGTCTCCG GCACAGCAGG AGCCCCCACA GAGGCAAACC 
GTTGGAACCA AACAACCCAA AAAACCTGTC AAGGCCTCTG CCCGGGCAGG TTCACGGGCC 
AGCCTGCAGG GGGAAAGGGA GCCAGGGCTT CTTCCCTACG GCTCCCGAGA CCAGACTTCC 
AAAGACAAGC CCAAGGTGAA GACGAAAGGA CGGCCCCGGG CCGCAGCAAG CAACGAGCCC 
AAGCCAGCAG TGCCCCCCTC CAGTGAGAAG AAGAAGCACA AGAGCTCCCT CCCTGCTCCC 
TCTAAGGCTC CCTCAGGCCC AGAACCCACG AAGGACAATG TGGAGGACAG GAGCCCTGAG 
CACTTTGCTC TTGTTCCCCT GACTCAGAGC CAGGGCCCAC CCCACAGTGG CAGCGGCAGC 
GGCAGCAGGA CTAGTGGCTG CCGCCAAGCC GTGGTGGTCC AGGAGGACAG CCGCAAAGAC 
AGACTCCCAT TGCCTTTGAG AGACACCAAG CTGCTCTCAC CACTCAGGGA CACTCCTCCC 
CCACAAAGCT TGATGGTGAA GATCACCCTA GACCTGCTCT CTCGGATACC CCAGCCTCCC 
GGGAAGAGGA GCCGCCAGAG GAAAGCAGAA GATAAACAGC CACCCGCAGG GAAGAAGCAC 
AGCTCTGAGA AGAGGAGCTC AGACAGCTCA AGCAAGTTGG CCAAAAAGAG AAAGGGTGAA 
GCAGAAAGAG ACTGTGATAA CAAGAAAATC AGACTGGAGA AGGAAATCAA ATCACAGTCA 
TCTTCATCTT CATCCTCCCA CAAAGAATCT TCTAAAACAA AGCCCTCCTC ACAGTCCTCA 
AAGAAGGAAA TGCTCCCCCC GCCACCCGTG TCCTCGTCCT CTCAGAAGCC AGCCAAGCCT 
GCACATAAGA GGTCAAGGCG GGAAGCAGAC ACCTGTGGCC AGGACCCTCC CAAAAGTGCC 
AGCAGTACCA AGAGCAACCA CAAAGACTCT TCCATTCCCA AGCAGAGAAG AGTAGAGGGG 
AAGGGCTCCA GAAGCTCCTC GGAGCACAAG GGTTCTTCCG GAGATACTGC AAATCCTTTT 
CCAGTGCCTT CTTTGCCAAA TGGTAACTCT AAACCAGGGA AGCCTCAAGT GAAGTTTGAC 
AAACAACAAG CAGACCTTCA CATGAGGGAG GCAAAAAAGA TGAAGCAGAA AGCAGAGTTA 
ATGACGGACA AGGTTGGAAA GGCTTTTAAG TACCTGGAAG CCGTCTTGTC CTTCATCGAG 
TGTGGAATTG CTACAGAGTC TGAAAGCCAG GCATCCAAGT CAGCTTACTC TGTCTACTCA 
GAAACTGTAG ATCTCATTAA ATTCATAATG TCATTAAAAT CCTTCTCAGA TGCCACAGCG 
CCAACACAAG AGAAAATATT TGCCGTTTTA TGCATGCGTT GCCAGTCCAT TTTGAACATG 
GCGATGTTTC GTTGTAAAAA AGACATAGCA ATAAAGTATT CTCGTACTCT TAATAAACAC 
TTCGAGAGTT CTTCCAAAGT CGCCCAGGCA CCTTCTCCAT GCATTGCAAG AAGCACAGGC 
ACACCATCCC CTCTTTCCCC AATGCCTTCT CCTGCCAGCT CCGTAGGGTC CCAGTCAAGT 
GCTGGCAGTG TGGGGAGCAG TGGGGTGGCT GCCACTATCA GCACCCCAGT CACCATCCAG 
AATATGACAT CTTCCTATGT CACCATCACG TCCCATGTTC TTACTGCCTT TGACCTTTGG 
GAACAGGCCG AGGCCCTCAC AAGGAAGAAT AAAGAATTCT TTGCTCGGCT CAGCACAAAT 
GTGTGCACCT TGGCCCTCAA CAGCAGTTTG GTGGACCTGG TGCACTATAC ACGACAGGGT 
TTTCAGCAGC TACAAGAATT AACCAAAACA CCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_024245835.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024101603.1 Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OSa180857
Clone ID Related Accession (Same CDS sequence)	XM_024245835.1
Accession Version	XM_024245835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	AF4/FMR2 family member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGCAGACCTA CTCCAATGAA 
GTCCATTGTG TTGAAGAGAT TCTGAAGGAC TCTCAGCACG TCAGTTCTGT AACCCAAAAC 
CAAAAACAAT ATGATACATC TTCAAAAACT CACTCAAATT CTCAGCAAGG AACGTCATCC 
ATGCTCGAAG ATGACCTTCA GCTCAGTGAC AGTGAGGACA GTGACAGTGA ACAAACCCCA 
GAGAAGCCTC CCTCCTCATC TGCACCTCCA AGTGCTCCAC AGTCCCTTCC AGAACCAGTG 
GCATCAGCAC ATTCCAGCAG TGCAGAGTCA GAAAGCACCA GTGACTCAGA CAGTTCCTCA 
GACTCGGAGA GCGAGAGCAG TTCAAGTGAC AGCGAAGAAA ATGAGCCCCT AGAAACCCCG 
GCTCCGGAGC CTGAGCCTCC AACAACAAAC AAATGGCAGC TGGACAACTG GCTGACCAAA 
GTCAGCCAGC CAGCTGCGCC ACTGGAGGGC CCCGGAAGCA CAGAGCCCCC ACGGCGGCAC 
CCAGAGAGTA AGGGCAGCAG TGACAGTGCC ACGAGTCAGG AGCATTCTGA ATCCAAAGAT 
CCTCCCCCTA AAAGCTCCAG CAAAGCCCTC CGGGCCCCAC CCGAAGCCCC CCACCCCGGA 
AAGAGGAGCT GTCAGAAGTC TCCGGCACAG CAGGAGCCCC CACAGAGGCA AACCGTTGGA 
ACCAAACAAC CCAAAAAACC TGTCAAGGCC TCTGCCCGGG CAGGTTCACG GGCCAGCCTG 
CAGGGGGAAA GGGAGCCAGG GCTTCTTCCC TACGGCTCCC GAGACCAGAC TTCCAAAGAC 
AAGCCCAAGG TGAAGACGAA AGGACGGCCC CGGGCCGCAG CAAGCAACGA GCCCAAGCCA 
GCAGTGCCCC CCTCCAGTGA GAAGAAGAAG CACAAGAGCT CCCTCCCTGC TCCCTCTAAG 
GCTCCCTCAG GCCCAGAACC CACGAAGGAC AATGTGGAGG ACAGGAGCCC TGAGCACTTT 
GCTCTTGTTC CCCTGACTCA GAGCCAGGGC CCACCCCACA GTGGCAGCGG CAGCGGCAGC 
AGGACTAGTG GCTGCCGCCA AGCCGTGGTG GTCCAGGAGG ACAGCCGCAA AGACAGACTC 
CCATTGCCTT TGAGAGACAC CAAGCTGCTC TCACCACTCA GGGACACTCC TCCCCCACAA 
AGCTTGATGG TGAAGATCAC CCTAGACCTG CTCTCTCGGA TACCCCAGCC TCCCGGGAAG 
AGGAGCCGCC AGAGGAAAGC AGAAGATAAA CAGCCACCCG CAGGGAAGAA GCACAGCTCT 
GAGAAGAGGA GCTCAGACAG CTCAAGCAAG TTGGCCAAAA AGAGAAAGGG TGAAGCAGAA 
AGAGACTGTG ATAACAAGAA AATCAGACTG GAGAAGGAAA TCAAATCACA GTCATCTTCA 
TCTTCATCCT CCCACAAAGA ATCTTCTAAA ACAAAGCCCT CCTCACAGTC CTCAAAGAAG 
GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCTCAGA AGCCAGCCAA GCCTGCACAT 
AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT 
ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC 
TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG 
CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA 
CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG 
GACAAGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT CGAGTGTGGA 
ATTGCTACAG AGTCTGAAAG CCAGGCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT 
GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA 
CAAGAGAAAA TATTTGCCGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG 
TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG 
AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA 
TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC 
AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG 
ACATCTTCCT ATGTCACCAT CACGTCCCAT GTTCTTACTG CCTTTGACCT TTGGGAACAG 
GCCGAGGCCC TCACAAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC 
ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG 
CAGCTACAAG AATTAACCAA AACACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024101603.1
CDS	290..3859
Translation	MAFTERVNSSGNSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDAPENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPEREPSPLISSLPSPVPPLSPIHSNQQTLPRTQGSSKVHGSNNNSKGCCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYSNEVHCVEEILKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPLEGPGSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKALRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRASLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKAPSGPEPTKDNVEDRSPEHFALVPLTQSQGPPHSGSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKRSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSSQSSKKEMLPPPPVSSSSQKPAKPAHKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDKVGKAFKYLEAVLSFIECGIATESESQASKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIARSTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Target ORF information:

RefSeq Version	XM_024245835.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024245835.1

ORF Insert Sequence:

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGCAGACCTA CTCCAATGAA 
GTCCATTGTG TTGAAGAGAT TCTGAAGGAC TCTCAGCACG TCAGTTCTGT AACCCAAAAC 
CAAAAACAAT ATGATACATC TTCAAAAACT CACTCAAATT CTCAGCAAGG AACGTCATCC 
ATGCTCGAAG ATGACCTTCA GCTCAGTGAC AGTGAGGACA GTGACAGTGA ACAAACCCCA 
GAGAAGCCTC CCTCCTCATC TGCACCTCCA AGTGCTCCAC AGTCCCTTCC AGAACCAGTG 
GCATCAGCAC ATTCCAGCAG TGCAGAGTCA GAAAGCACCA GTGACTCAGA CAGTTCCTCA 
GACTCGGAGA GCGAGAGCAG TTCAAGTGAC AGCGAAGAAA ATGAGCCCCT AGAAACCCCG 
GCTCCGGAGC CTGAGCCTCC AACAACAAAC AAATGGCAGC TGGACAACTG GCTGACCAAA 
GTCAGCCAGC CAGCTGCGCC ACTGGAGGGC CCCGGAAGCA CAGAGCCCCC ACGGCGGCAC 
CCAGAGAGTA AGGGCAGCAG TGACAGTGCC ACGAGTCAGG AGCATTCTGA ATCCAAAGAT 
CCTCCCCCTA AAAGCTCCAG CAAAGCCCTC CGGGCCCCAC CCGAAGCCCC CCACCCCGGA 
AAGAGGAGCT GTCAGAAGTC TCCGGCACAG CAGGAGCCCC CACAGAGGCA AACCGTTGGA 
ACCAAACAAC CCAAAAAACC TGTCAAGGCC TCTGCCCGGG CAGGTTCACG GGCCAGCCTG 
CAGGGGGAAA GGGAGCCAGG GCTTCTTCCC TACGGCTCCC GAGACCAGAC TTCCAAAGAC 
AAGCCCAAGG TGAAGACGAA AGGACGGCCC CGGGCCGCAG CAAGCAACGA GCCCAAGCCA 
GCAGTGCCCC CCTCCAGTGA GAAGAAGAAG CACAAGAGCT CCCTCCCTGC TCCCTCTAAG 
GCTCCCTCAG GCCCAGAACC CACGAAGGAC AATGTGGAGG ACAGGAGCCC TGAGCACTTT 
GCTCTTGTTC CCCTGACTCA GAGCCAGGGC CCACCCCACA GTGGCAGCGG CAGCGGCAGC 
AGGACTAGTG GCTGCCGCCA AGCCGTGGTG GTCCAGGAGG ACAGCCGCAA AGACAGACTC 
CCATTGCCTT TGAGAGACAC CAAGCTGCTC TCACCACTCA GGGACACTCC TCCCCCACAA 
AGCTTGATGG TGAAGATCAC CCTAGACCTG CTCTCTCGGA TACCCCAGCC TCCCGGGAAG 
AGGAGCCGCC AGAGGAAAGC AGAAGATAAA CAGCCACCCG CAGGGAAGAA GCACAGCTCT 
GAGAAGAGGA GCTCAGACAG CTCAAGCAAG TTGGCCAAAA AGAGAAAGGG TGAAGCAGAA 
AGAGACTGTG ATAACAAGAA AATCAGACTG GAGAAGGAAA TCAAATCACA GTCATCTTCA 
TCTTCATCCT CCCACAAAGA ATCTTCTAAA ACAAAGCCCT CCTCACAGTC CTCAAAGAAG 
GAAATGCTCC CCCCGCCACC CGTGTCCTCG TCCTCTCAGA AGCCAGCCAA GCCTGCACAT 
AAGAGGTCAA GGCGGGAAGC AGACACCTGT GGCCAGGACC CTCCCAAAAG TGCCAGCAGT 
ACCAAGAGCA ACCACAAAGA CTCTTCCATT CCCAAGCAGA GAAGAGTAGA GGGGAAGGGC 
TCCAGAAGCT CCTCGGAGCA CAAGGGTTCT TCCGGAGATA CTGCAAATCC TTTTCCAGTG 
CCTTCTTTGC CAAATGGTAA CTCTAAACCA GGGAAGCCTC AAGTGAAGTT TGACAAACAA 
CAAGCAGACC TTCACATGAG GGAGGCAAAA AAGATGAAGC AGAAAGCAGA GTTAATGACG 
GACAAGGTTG GAAAGGCTTT TAAGTACCTG GAAGCCGTCT TGTCCTTCAT CGAGTGTGGA 
ATTGCTACAG AGTCTGAAAG CCAGGCATCC AAGTCAGCTT ACTCTGTCTA CTCAGAAACT 
GTAGATCTCA TTAAATTCAT AATGTCATTA AAATCCTTCT CAGATGCCAC AGCGCCAACA 
CAAGAGAAAA TATTTGCCGT TTTATGCATG CGTTGCCAGT CCATTTTGAA CATGGCGATG 
TTTCGTTGTA AAAAAGACAT AGCAATAAAG TATTCTCGTA CTCTTAATAA ACACTTCGAG 
AGTTCTTCCA AAGTCGCCCA GGCACCTTCT CCATGCATTG CAAGAAGCAC AGGCACACCA 
TCCCCTCTTT CCCCAATGCC TTCTCCTGCC AGCTCCGTAG GGTCCCAGTC AAGTGCTGGC 
AGTGTGGGGA GCAGTGGGGT GGCTGCCACT ATCAGCACCC CAGTCACCAT CCAGAATATG 
ACATCTTCCT ATGTCACCAT CACGTCCCAT GTTCTTACTG CCTTTGACCT TTGGGAACAG 
GCCGAGGCCC TCACAAGGAA GAATAAAGAA TTCTTTGCTC GGCTCAGCAC AAATGTGTGC 
ACCTTGGCCC TCAACAGCAG TTTGGTGGAC CTGGTGCACT ATACACGACA GGGTTTTCAG 
CAGCTACAAG AATTAACCAA AACACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_024245836.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_024101604.1 Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X6, mRNA.

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CloneID	OSa180858
Clone ID Related Accession (Same CDS sequence)	XM_024245836.1
Accession Version	XM_024245836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	AF4/FMR2 family member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036907.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGACCTACTC CAATGAAGTC 
CATTGTGTTG AAGAGATTCT GAAGGACTCT CAGCACGTCA GTTCTGTAAC CCAAAACCAA 
AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG 
CTCGAAGATG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG 
AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA 
TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC 
TCGGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCGGCT 
CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC 
AGCCAGCCAG CTGCGCCACT GGAGGGCCCC GGAAGCACAG AGCCCCCACG GCGGCACCCA 
GAGAGTAAGG GCAGCAGTGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT 
CCCCCTAAAA GCTCCAGCAA AGCCCTCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG 
AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AGAGGCAAAC CGTTGGAACC 
AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGGC CAGCCTGCAG 
GGGGAAAGGG AGCCAGGGCT TCTTCCCTAC GGCTCCCGAG ACCAGACTTC CAAAGACAAG 
CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAGCC CAAGCCAGCA 
GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCTCC CTCTAAGGCT 
CCCTCAGGCC CAGAACCCAC GAAGGACAAT GTGGAGGACA GGAGCCCTGA GCACTTTGCT 
CTTGTTCCCC TGACTCAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CGGCAGCAGG 
ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC CAGGAGGACA GCCGCAAAGA CAGACTCCCA 
TTGCCTTTGA GAGACACCAA GCTGCTCTCA CCACTCAGGG ACACTCCTCC CCCACAAAGC 
TTGATGGTGA AGATCACCCT AGACCTGCTC TCTCGGATAC CCCAGCCTCC CGGGAAGAGG 
AGCCGCCAGA GGAAAGCAGA AGATAAACAG CCACCCGCAG GGAAGAAGCA CAGCTCTGAG 
AAGAGGAGCT CAGACAGCTC AAGCAAGTTG GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA 
GACTGTGATA ACAAGAAAAT CAGACTGGAG AAGGAAATCA AATCACAGTC ATCTTCATCT 
TCATCCTCCC ACAAAGAATC TTCTAAAACA AAGCCCTCCT CACAGTCCTC AAAGAAGGAA 
ATGCTCCCCC CGCCACCCGT GTCCTCGTCC TCTCAGAAGC CAGCCAAGCC TGCACATAAG 
AGGTCAAGGC GGGAAGCAGA CACCTGTGGC CAGGACCCTC CCAAAAGTGC CAGCAGTACC 
AAGAGCAACC ACAAAGACTC TTCCATTCCC AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC 
AGAAGCTCCT CGGAGCACAA GGGTTCTTCC GGAGATACTG CAAATCCTTT TCCAGTGCCT 
TCTTTGCCAA ATGGTAACTC TAAACCAGGG AAGCCTCAAG TGAAGTTTGA CAAACAACAA 
GCAGACCTTC ACATGAGGGA GGCAAAAAAG ATGAAGCAGA AAGCAGAGTT AATGACGGAC 
AAGGTTGGAA AGGCTTTTAA GTACCTGGAA GCCGTCTTGT CCTTCATCGA GTGTGGAATT 
GCTACAGAGT CTGAAAGCCA GGCATCCAAG TCAGCTTACT CTGTCTACTC AGAAACTGTA 
GATCTCATTA AATTCATAAT GTCATTAAAA TCCTTCTCAG ATGCCACAGC GCCAACACAA 
GAGAAAATAT TTGCCGTTTT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCGATGTTT 
CGTTGTAAAA AAGACATAGC AATAAAGTAT TCTCGTACTC TTAATAAACA CTTCGAGAGT 
TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA TGCATTGCAA GAAGCACAGG CACACCATCC 
CCTCTTTCCC CAATGCCTTC TCCTGCCAGC TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT 
GTGGGGAGCA GTGGGGTGGC TGCCACTATC AGCACCCCAG TCACCATCCA GAATATGACA 
TCTTCCTATG TCACCATCAC GTCCCATGTT CTTACTGCCT TTGACCTTTG GGAACAGGCC 
GAGGCCCTCA CAAGGAAGAA TAAAGAATTC TTTGCTCGGC TCAGCACAAA TGTGTGCACC 
TTGGCCCTCA ACAGCAGTTT GGTGGACCTG GTGCACTATA CACGACAGGG TTTTCAGCAG 
CTACAAGAAT TAACCAAAAC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024101604.1
CDS	290..3856
Translation	MAFTERVNSSGNSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDAPENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPEREPSPLISSLPSPVPPLSPIHSNQQTLPRTQGSSKVHGSNNNSKGCCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVETYSNEVHCVEEILKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPLEGPGSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKALRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRASLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKAPSGPEPTKDNVEDRSPEHFALVPLTQSQGPPHSGSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKRSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSSQSSKKEMLPPPPVSSSSQKPAKPAHKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDKVGKAFKYLEAVLSFIECGIATESESQASKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIARSTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Target ORF information:

RefSeq Version	XM_024245836.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii AF4/FMR2 family member 1 (AFF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024245836.1

ORF Insert Sequence:

ATGGCATTTA CGGAAAGAGT CAACAGCAGT GGCAACAGTT TGTACAATGA CGACAGAAAC 
CTGCTTCGAA TTAGAGAGAA GGAAAGACGC AACCAGGAAG CCCACCAAGA GAAAGAGGCA 
TTTCCTGAAA AGATTCCCCT TTTTGGAGAG CCCTACAAGA CAGCAAAAGG TGATGAGCTG 
TCTAGTCGAA TACAGAACAT GTTGGGAAAC TACGAAGAAG TGAAGGAGTT CCTTAGTACT 
AAGTCTCACA CTCATCGCTT GGATGCTCCT GAAAATAGGT TGGGAAAGCC AAAATATCCT 
TTAATTCCTG ACAAAGGGAG CAGCATTCCA TCCAGCTCCT TCCACACTAG TGTCCACCAC 
CAGTCCATTC ACACTCCTGC GTCTGGACCA CTTTCTGTTG GCAACATTAG CCACAATCCA 
AAGATGGCGC AGCCAAGAAC TGAACCAATG CCAAGTCTCC ATGCCAAAAG CTGTGGCCCA 
CCGGACAGCC AGCACCTGAC CCAGGATCGC CTTGGTCAGG AGGGGTTCGG CTCTAGTCAT 
CACAAGAAAG GTGACCGAAG AGCTGACGGA GACCACTGTG CTTCGGTGAC AGACTCGGCT 
CCAGAGAGGG AGCCTTCTCC CTTAATCTCC TCTTTGCCTT CCCCAGTTCC CCCTTTGTCA 
CCTATACATT CCAACCAGCA AACTCTTCCC CGGACGCAAG GAAGCAGCAA GGTTCATGGC 
AGCAACAATA ACAGTAAAGG CTGTTGCCCA GCCAAATCTC CCAAGGACCT AGCAGTGAAA 
GTCCATGATA AAGAGACCCC TCAAGACAGT TTGGTGGCCC CTGCCCAGCC GCCTTCTCAG 
ACATTTCCAC CTCCCTCCCT CCCCTCAAAA AGTGTTGCAA TGCAGCAGAA GCCCACGGCT 
TATGTCCGGC CCATGGATGG TCAAGATCAG GCCCCTAGTG AATCCCCTGA ACTGAAACCA 
CTGCCGGAGG ACTATCGACA GCAGACCTTT GAAAAAACAG ACTTGAAAGT GCCTGCCAAA 
GCCAAGCTCA CCAAACTGAA GATGCCTTCT CAGTCAGTTG AGACCTACTC CAATGAAGTC 
CATTGTGTTG AAGAGATTCT GAAGGACTCT CAGCACGTCA GTTCTGTAAC CCAAAACCAA 
AAACAATATG ATACATCTTC AAAAACTCAC TCAAATTCTC AGCAAGGAAC GTCATCCATG 
CTCGAAGATG ACCTTCAGCT CAGTGACAGT GAGGACAGTG ACAGTGAACA AACCCCAGAG 
AAGCCTCCCT CCTCATCTGC ACCTCCAAGT GCTCCACAGT CCCTTCCAGA ACCAGTGGCA 
TCAGCACATT CCAGCAGTGC AGAGTCAGAA AGCACCAGTG ACTCAGACAG TTCCTCAGAC 
TCGGAGAGCG AGAGCAGTTC AAGTGACAGC GAAGAAAATG AGCCCCTAGA AACCCCGGCT 
CCGGAGCCTG AGCCTCCAAC AACAAACAAA TGGCAGCTGG ACAACTGGCT GACCAAAGTC 
AGCCAGCCAG CTGCGCCACT GGAGGGCCCC GGAAGCACAG AGCCCCCACG GCGGCACCCA 
GAGAGTAAGG GCAGCAGTGA CAGTGCCACG AGTCAGGAGC ATTCTGAATC CAAAGATCCT 
CCCCCTAAAA GCTCCAGCAA AGCCCTCCGG GCCCCACCCG AAGCCCCCCA CCCCGGAAAG 
AGGAGCTGTC AGAAGTCTCC GGCACAGCAG GAGCCCCCAC AGAGGCAAAC CGTTGGAACC 
AAACAACCCA AAAAACCTGT CAAGGCCTCT GCCCGGGCAG GTTCACGGGC CAGCCTGCAG 
GGGGAAAGGG AGCCAGGGCT TCTTCCCTAC GGCTCCCGAG ACCAGACTTC CAAAGACAAG 
CCCAAGGTGA AGACGAAAGG ACGGCCCCGG GCCGCAGCAA GCAACGAGCC CAAGCCAGCA 
GTGCCCCCCT CCAGTGAGAA GAAGAAGCAC AAGAGCTCCC TCCCTGCTCC CTCTAAGGCT 
CCCTCAGGCC CAGAACCCAC GAAGGACAAT GTGGAGGACA GGAGCCCTGA GCACTTTGCT 
CTTGTTCCCC TGACTCAGAG CCAGGGCCCA CCCCACAGTG GCAGCGGCAG CGGCAGCAGG 
ACTAGTGGCT GCCGCCAAGC CGTGGTGGTC CAGGAGGACA GCCGCAAAGA CAGACTCCCA 
TTGCCTTTGA GAGACACCAA GCTGCTCTCA CCACTCAGGG ACACTCCTCC CCCACAAAGC 
TTGATGGTGA AGATCACCCT AGACCTGCTC TCTCGGATAC CCCAGCCTCC CGGGAAGAGG 
AGCCGCCAGA GGAAAGCAGA AGATAAACAG CCACCCGCAG GGAAGAAGCA CAGCTCTGAG 
AAGAGGAGCT CAGACAGCTC AAGCAAGTTG GCCAAAAAGA GAAAGGGTGA AGCAGAAAGA 
GACTGTGATA ACAAGAAAAT CAGACTGGAG AAGGAAATCA AATCACAGTC ATCTTCATCT 
TCATCCTCCC ACAAAGAATC TTCTAAAACA AAGCCCTCCT CACAGTCCTC AAAGAAGGAA 
ATGCTCCCCC CGCCACCCGT GTCCTCGTCC TCTCAGAAGC CAGCCAAGCC TGCACATAAG 
AGGTCAAGGC GGGAAGCAGA CACCTGTGGC CAGGACCCTC CCAAAAGTGC CAGCAGTACC 
AAGAGCAACC ACAAAGACTC TTCCATTCCC AAGCAGAGAA GAGTAGAGGG GAAGGGCTCC 
AGAAGCTCCT CGGAGCACAA GGGTTCTTCC GGAGATACTG CAAATCCTTT TCCAGTGCCT 
TCTTTGCCAA ATGGTAACTC TAAACCAGGG AAGCCTCAAG TGAAGTTTGA CAAACAACAA 
GCAGACCTTC ACATGAGGGA GGCAAAAAAG ATGAAGCAGA AAGCAGAGTT AATGACGGAC 
AAGGTTGGAA AGGCTTTTAA GTACCTGGAA GCCGTCTTGT CCTTCATCGA GTGTGGAATT 
GCTACAGAGT CTGAAAGCCA GGCATCCAAG TCAGCTTACT CTGTCTACTC AGAAACTGTA 
GATCTCATTA AATTCATAAT GTCATTAAAA TCCTTCTCAG ATGCCACAGC GCCAACACAA 
GAGAAAATAT TTGCCGTTTT ATGCATGCGT TGCCAGTCCA TTTTGAACAT GGCGATGTTT 
CGTTGTAAAA AAGACATAGC AATAAAGTAT TCTCGTACTC TTAATAAACA CTTCGAGAGT 
TCTTCCAAAG TCGCCCAGGC ACCTTCTCCA TGCATTGCAA GAAGCACAGG CACACCATCC 
CCTCTTTCCC CAATGCCTTC TCCTGCCAGC TCCGTAGGGT CCCAGTCAAG TGCTGGCAGT 
GTGGGGAGCA GTGGGGTGGC TGCCACTATC AGCACCCCAG TCACCATCCA GAATATGACA 
TCTTCCTATG TCACCATCAC GTCCCATGTT CTTACTGCCT TTGACCTTTG GGAACAGGCC 
GAGGCCCTCA CAAGGAAGAA TAAAGAATTC TTTGCTCGGC TCAGCACAAA TGTGTGCACC 
TTGGCCCTCA ACAGCAGTTT GGTGGACCTG GTGCACTATA CACGACAGGG TTTTCAGCAG 
CTACAAGAAT TAACCAAAAC ACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFF1 ( XM_009240181.1 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_009238456.1 Pongo abelii AF4/FMR2 family, member 1 (AFF1), mRNA.

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CloneID	OSa35184
Clone ID Related Accession (Same CDS sequence)	XM_009240181.1
Accession Version	XM_009240181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	LOW QUALITY PROTEIN: AF4/FMR2 family member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002878734.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

ATGGCAGCCC AGTCAAGTTT GTACAATGAC GACAGAAACC TTGGCTTCCG AATTAGAGAG 
AAGGAAAGAC GCAACCAGGA AGCCCACCAA GAGAAAGAGG CATTTCCTGA AAAGATTCCC 
CTTTTTGGAG AGCCCTACAA GACAGCAAAA GGTGATGAGC TGTCTAGTCG AATACAGAAC 
ATGTTGGGAA ACTACGAAGA AGTGAAGGAG TTCCTTAGTA CTAAGTCTCA CACTCATCGC 
TTGGATGCTC CTGAAAATGC GTTGGGAAAG CCAAAATATC CTTTAATTCC TGACAAAGGG 
AGCAGCATTC CATCCAGCTC CTTCCACACT AGTGTCCACC ACCAGTCCAT TCACACTCCT 
GCGTCTGGAC CACTTTCTGT TGGCAGCATT AGCCACAATC CAAAGATGGC GCAGCCAAGA 
ACTGAACCAA TGCCAAGTCT CCATGCCAAA AGCTGTGGCC CACCGGACAG CCAGCACCTG 
ACCCAGGATC GCCTTGGTCA GGAGGGGTTC GGCTCTAGTC ATCACAAGAA AGGTGACCGA 
AGAGCTGACG GAGACCACTG TGCTTCGGTG ACAGACTCGG CTCGCAGAGA GGGAGCCTTC 
TCCCTTAATC TCCTCTTTGC CTTCCCCACA GTTCCCCCTT TGTCACCTAT ACATTCCAAC 
CAGCAAACTC TTCCCCGGAC GCAAGGAAGC AGCAAGGTTC ATGGCAGCAA CAATAACAGT 
AAAGGCTGTT GCCCAGCCAA ATCTCCCAAG GACCTAGCAG TGAAAGTCCA TGATAAAGAG 
ACCCCTCAAG ACAGTTTGGT GGCCCCTGCC CAGCCGCCTT CTCAGACATT TCCACCTCCC 
TCCCTCCCCT CAAAAAGTGT TGCAATGCAG CAGAAGCCCA CGGCTTATGT CCGGCCCATG 
GATGGTCAAG ATCAGGCCCC TAGTGAATCC CCTGAACTGA AACCACTGCC GGAGGACTAT 
CGACAGCAGA CCTTTGAAAA AACAGACTTG AAAGTGCCTG CCAAAGCCAA GCTCACCAAA 
CTGAAGATGC CTTCTCAGTC AGTTGAGACC TACTCCAATG AAGTCCATTG TGTTGAAGAG 
ATTCTGAAGG AAATGACCCA TTCATGGCCT CCTCCTTTGA CAGCAATACA TACGCCTAGT 
ACAGCTGAGC CATCCAAGTT TCCTTTCCCC ACAAAGGACT CTCAGCACGT CAGTTCTGTA 
ACCCAAAACC AAAAACAATA TGATACATCT TCAAAAACTC ACTCAAATTC TCAGCAAGGA 
ACGTCATCCA TGCTCGAAGA TGACCTTCAG CTCAGTGACA GTGAGGACAG TGACAGTGAA 
CAAACCCCAG AGAAGCCTCC CTCCTCATCT GCACCTCCAA GTGCTCCACA GTCCCTTCCA 
GAACCAGTGG CATCAGCACA TTCCAGCAGT GCAGAGTCAG AAAGCACCAG TGACTCAGAC 
AGTTCCTCAG ACTCGGAGAG CGAGAGCAGT TCAAGTGACA GCGAAGAAAA TGAGCCCCTA 
GAAACCCCGG CTCCGGAGCC TGAGCCTCCA ACAACAAACA AATGGCAGCT GGACAACTGG 
CTGACCAAAG TCAGCCAGCC AGCTGCGCCA CTGGAGGGCC CCGGAAGCAC AGAGCCCCCA 
CGGCGGCACC CAGAGAGTAA GGGCAGCAGT GACAGTGCCA CGAGTCAGGA GCATTCTGAA 
TCCAAAGATC CTCCCCCTAA AAGCTCCAGC AAAGCCCTCC GGGCCCCACC CGAAGCCCCC 
CACCCCGGAA AGAGGAGCTG TCAGAAGTCT CCGGCACAGC AGGAGCCCCC ACAGAGGCAA 
ACCGTTGGAA CCAAACAACC CAAAAAACCT GTCAAGGCCT CTGCCCGGGC AGGTTCACGG 
GCCAGCCTGC AGGGGGAAAG GGAGCCAGGG CTTCTTCCCT ACGGCTCCCG AGACCAGACT 
TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGACGGCCCC GGGCCGCAGC AAGCAACGAG 
CCCAAGCCAG CAGTGCCCCC CTCCAGTGAG AAGAAGAAGC ACAAGAGCTC CCTCCCTGCT 
CCCTCTAAGG CTCCCTCAGG CCCAGAACCC ACGAAGGACA ATGTGGAGGA CAGGAGCCCT 
GAGCACTTTG CTCTTGTTCC CCTGACTCAG AGCCAGGGCC CACCCCACAG TGGCAGCGGC 
AGCGGCAGCA GGACTAGTGG CTGCCGCCAA GCCGTGGTGG TCCAGGAGGA CAGCCGCAAA 
GACAGACTCC CATTGCCTTT GAGAGACACC AAGCTGCTCT CACCACTCAG GGACACTCCT 
CCCCCACAAA GCTTGATGGT GAAGATCACC CTAGACCTGC TCTCTCGGAT ACCCCAGCCT 
CCCGGGAAGA GGAGCCGCCA GAGGAAAGCA GAAGATAAAC AGCCACCCGC AGGGAAGAAG 
CACAGCTCTG AGAAGAGGAG CTCAGACAGC TCAAGCAAGT TGGCCAAAAA GAGAAAGGGT 
GAAGCAGAAA GAGACTGTGA TAACAAGAAA ATCAGACTGG AGAAGGAAAT CAAATCACAG 
TCATCTTCAT CTTCATCCTC CCACAAAGAA TCTTCTAAAA CAAAAGAAGC CAGCCCAAGC 
CCTGCACATA AGAGGTCAAG GCGGGAAGCA GACACCTGTT GCAGGACCCT CCCAAAAAGT 
GCCAGCAGTA CCAAGAGCAA CCACAAAGAC TCTTCCATTC CCAAGCAGAG AAGAGTAGAG 
GGGAAGGGCT CCAGAAGCTC CTCGGAGCAC AAGGGTTCTT CCGGAGATAC TGCAAATCCT 
TTTCCAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCA AGTGAAGTTT 
GACAAACAAC AAGCAGACCT TCACATGAGG GAGGCAAAAA AGATGAAGCA GAAAGCAGAG 
TTAATGACGG ACAAGGTTGG AAAGGCTTTT AAGTACCTGG AAGCCGTCTT GTCCTTCATC 
GAGTGTGGAA TTGCTACAGA GTCTGAAAGC CAGGCATCCA AGTCAGCTTA CTCTGTCTAC 
TCAGAAACTG TAGATCTCAT TAAATTCATA ATGTCATTAA AATCCTTCTC AGATGCCACA 
GCGCCAACAC AAGAGAAAAT ATTTGCCGTT TTATGCATGC GTTGCCAGTC CATTTTGAAC 
ATGGCGATGT TTCGTTGTAA AAAAGACATA GCAATAAAGT ATTCTCGTAC TCTTAATAAA 
CACTTCGAGA GTTCTTCCAA AGTCGCCCAG GCACCTTCTC CATGCATTGC AAGAAGCACA 
GGCACACCAT CCCCTCTTTC CCCAATGCCT TCTCCTGCCA GCTCCGTAGG GTCCCAGTCA 
AGTGCTGGCA GTGTGGGGAG CAGTGGGGTG GCTGCCACTA TCAGCACCCC AGTCACCATC 
CAGAATATGA CATCTTCCTA TGTCACCATC ACGTCCCATG TTCTTACTGC CTTTGACCTT 
TGGGAACAGG CCGAGGCCCT CACAAGGAAG AATAAAGAAT TCTTTGCTCG GCTCAGCACA 
AATGTGTGCA CCTTGGCCCT CAACAGCAGT TTGGTGGACC TGGTGCACTA TACACGACAG 
GGTTTTCAGC AGCTACAAGA ATTAACCAAA ACACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009238456.1
CDS	406..3984
Translation	MAAQSSLYNDDRNLGFRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDAPENALGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGSISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSARREGAFSLNLLFAFPTVPPLSPIHSNQQTLPRTQGSSKVHGSNNNSKGCCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVETYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPLEGPGSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKALRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRASLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKAPSGPEPTKDNVEDRSPEHFALVPLTQSQGPPHSGSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKRSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKEASPSPAHKRSRREADTCCRTLPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDKVGKAFKYLEAVLSFIECGIATESESQASKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIARSTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Target ORF information:

RefSeq Version	XM_009240181.1
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii AF4/FMR2 family, member 1 (AFF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009240181.1

ORF Insert Sequence:

ATGGCAGCCC AGTCAAGTTT GTACAATGAC GACAGAAACC TTGGCTTCCG AATTAGAGAG 
AAGGAAAGAC GCAACCAGGA AGCCCACCAA GAGAAAGAGG CATTTCCTGA AAAGATTCCC 
CTTTTTGGAG AGCCCTACAA GACAGCAAAA GGTGATGAGC TGTCTAGTCG AATACAGAAC 
ATGTTGGGAA ACTACGAAGA AGTGAAGGAG TTCCTTAGTA CTAAGTCTCA CACTCATCGC 
TTGGATGCTC CTGAAAATGC GTTGGGAAAG CCAAAATATC CTTTAATTCC TGACAAAGGG 
AGCAGCATTC CATCCAGCTC CTTCCACACT AGTGTCCACC ACCAGTCCAT TCACACTCCT 
GCGTCTGGAC CACTTTCTGT TGGCAGCATT AGCCACAATC CAAAGATGGC GCAGCCAAGA 
ACTGAACCAA TGCCAAGTCT CCATGCCAAA AGCTGTGGCC CACCGGACAG CCAGCACCTG 
ACCCAGGATC GCCTTGGTCA GGAGGGGTTC GGCTCTAGTC ATCACAAGAA AGGTGACCGA 
AGAGCTGACG GAGACCACTG TGCTTCGGTG ACAGACTCGG CTCGCAGAGA GGGAGCCTTC 
TCCCTTAATC TCCTCTTTGC CTTCCCCACA GTTCCCCCTT TGTCACCTAT ACATTCCAAC 
CAGCAAACTC TTCCCCGGAC GCAAGGAAGC AGCAAGGTTC ATGGCAGCAA CAATAACAGT 
AAAGGCTGTT GCCCAGCCAA ATCTCCCAAG GACCTAGCAG TGAAAGTCCA TGATAAAGAG 
ACCCCTCAAG ACAGTTTGGT GGCCCCTGCC CAGCCGCCTT CTCAGACATT TCCACCTCCC 
TCCCTCCCCT CAAAAAGTGT TGCAATGCAG CAGAAGCCCA CGGCTTATGT CCGGCCCATG 
GATGGTCAAG ATCAGGCCCC TAGTGAATCC CCTGAACTGA AACCACTGCC GGAGGACTAT 
CGACAGCAGA CCTTTGAAAA AACAGACTTG AAAGTGCCTG CCAAAGCCAA GCTCACCAAA 
CTGAAGATGC CTTCTCAGTC AGTTGAGACC TACTCCAATG AAGTCCATTG TGTTGAAGAG 
ATTCTGAAGG AAATGACCCA TTCATGGCCT CCTCCTTTGA CAGCAATACA TACGCCTAGT 
ACAGCTGAGC CATCCAAGTT TCCTTTCCCC ACAAAGGACT CTCAGCACGT CAGTTCTGTA 
ACCCAAAACC AAAAACAATA TGATACATCT TCAAAAACTC ACTCAAATTC TCAGCAAGGA 
ACGTCATCCA TGCTCGAAGA TGACCTTCAG CTCAGTGACA GTGAGGACAG TGACAGTGAA 
CAAACCCCAG AGAAGCCTCC CTCCTCATCT GCACCTCCAA GTGCTCCACA GTCCCTTCCA 
GAACCAGTGG CATCAGCACA TTCCAGCAGT GCAGAGTCAG AAAGCACCAG TGACTCAGAC 
AGTTCCTCAG ACTCGGAGAG CGAGAGCAGT TCAAGTGACA GCGAAGAAAA TGAGCCCCTA 
GAAACCCCGG CTCCGGAGCC TGAGCCTCCA ACAACAAACA AATGGCAGCT GGACAACTGG 
CTGACCAAAG TCAGCCAGCC AGCTGCGCCA CTGGAGGGCC CCGGAAGCAC AGAGCCCCCA 
CGGCGGCACC CAGAGAGTAA GGGCAGCAGT GACAGTGCCA CGAGTCAGGA GCATTCTGAA 
TCCAAAGATC CTCCCCCTAA AAGCTCCAGC AAAGCCCTCC GGGCCCCACC CGAAGCCCCC 
CACCCCGGAA AGAGGAGCTG TCAGAAGTCT CCGGCACAGC AGGAGCCCCC ACAGAGGCAA 
ACCGTTGGAA CCAAACAACC CAAAAAACCT GTCAAGGCCT CTGCCCGGGC AGGTTCACGG 
GCCAGCCTGC AGGGGGAAAG GGAGCCAGGG CTTCTTCCCT ACGGCTCCCG AGACCAGACT 
TCCAAAGACA AGCCCAAGGT GAAGACGAAA GGACGGCCCC GGGCCGCAGC AAGCAACGAG 
CCCAAGCCAG CAGTGCCCCC CTCCAGTGAG AAGAAGAAGC ACAAGAGCTC CCTCCCTGCT 
CCCTCTAAGG CTCCCTCAGG CCCAGAACCC ACGAAGGACA ATGTGGAGGA CAGGAGCCCT 
GAGCACTTTG CTCTTGTTCC CCTGACTCAG AGCCAGGGCC CACCCCACAG TGGCAGCGGC 
AGCGGCAGCA GGACTAGTGG CTGCCGCCAA GCCGTGGTGG TCCAGGAGGA CAGCCGCAAA 
GACAGACTCC CATTGCCTTT GAGAGACACC AAGCTGCTCT CACCACTCAG GGACACTCCT 
CCCCCACAAA GCTTGATGGT GAAGATCACC CTAGACCTGC TCTCTCGGAT ACCCCAGCCT 
CCCGGGAAGA GGAGCCGCCA GAGGAAAGCA GAAGATAAAC AGCCACCCGC AGGGAAGAAG 
CACAGCTCTG AGAAGAGGAG CTCAGACAGC TCAAGCAAGT TGGCCAAAAA GAGAAAGGGT 
GAAGCAGAAA GAGACTGTGA TAACAAGAAA ATCAGACTGG AGAAGGAAAT CAAATCACAG 
TCATCTTCAT CTTCATCCTC CCACAAAGAA TCTTCTAAAA CAAAAGAAGC CAGCCCAAGC 
CCTGCACATA AGAGGTCAAG GCGGGAAGCA GACACCTGTT GCAGGACCCT CCCAAAAAGT 
GCCAGCAGTA CCAAGAGCAA CCACAAAGAC TCTTCCATTC CCAAGCAGAG AAGAGTAGAG 
GGGAAGGGCT CCAGAAGCTC CTCGGAGCAC AAGGGTTCTT CCGGAGATAC TGCAAATCCT 
TTTCCAGTGC CTTCTTTGCC AAATGGTAAC TCTAAACCAG GGAAGCCTCA AGTGAAGTTT 
GACAAACAAC AAGCAGACCT TCACATGAGG GAGGCAAAAA AGATGAAGCA GAAAGCAGAG 
TTAATGACGG ACAAGGTTGG AAAGGCTTTT AAGTACCTGG AAGCCGTCTT GTCCTTCATC 
GAGTGTGGAA TTGCTACAGA GTCTGAAAGC CAGGCATCCA AGTCAGCTTA CTCTGTCTAC 
TCAGAAACTG TAGATCTCAT TAAATTCATA ATGTCATTAA AATCCTTCTC AGATGCCACA 
GCGCCAACAC AAGAGAAAAT ATTTGCCGTT TTATGCATGC GTTGCCAGTC CATTTTGAAC 
ATGGCGATGT TTCGTTGTAA AAAAGACATA GCAATAAAGT ATTCTCGTAC TCTTAATAAA 
CACTTCGAGA GTTCTTCCAA AGTCGCCCAG GCACCTTCTC CATGCATTGC AAGAAGCACA 
GGCACACCAT CCCCTCTTTC CCCAATGCCT TCTCCTGCCA GCTCCGTAGG GTCCCAGTCA 
AGTGCTGGCA GTGTGGGGAG CAGTGGGGTG GCTGCCACTA TCAGCACCCC AGTCACCATC 
CAGAATATGA CATCTTCCTA TGTCACCATC ACGTCCCATG TTCTTACTGC CTTTGACCTT 
TGGGAACAGG CCGAGGCCCT CACAAGGAAG AATAAAGAAT TCTTTGCTCG GCTCAGCACA 
AATGTGTGCA CCTTGGCCCT CAACAGCAGT TTGGTGGACC TGGTGCACTA TACACGACAG 
GGTTTTCAGC AGCTACAAGA ATTAACCAAA ACACCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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AFF1 cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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