POPTR_0001s35240g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene
Clones
gRNAs

Summary

Gene Symbol	POPTR_0001s35240g
Entrez Gene ID	7490707
Full Name	hypothetical protein
Synonyms	POPTRDRAFT_815510
Gene Type	protein-coding
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024609538.1	XP_024465306.1	protein RST1 isoform X1
XM_002300472.2	XP_002300508.2	hypothetical protein
XM_024609545.1	XP_024465313.1	protein RST1 isoform X2
XM_002300012.2	XP_002300048.2	hypothetical protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, D?jardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasj?rvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Lepl? JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouz? P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POPTR_0001s35240g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POPTR_0001s35240g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPa02203	XM_002300472.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0001s35240g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa02204	XM_002300012.2 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0001s35240g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa421716	XM_024609538.1 Latest version!	Populus trichocarpa protein RST1 (LOC7490707), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPa421717	XM_024609545.1 Latest version!	Populus trichocarpa protein RST1 (LOC7490707), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POPTR_0001s35240g ( XM_002300472.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002300508.2 Populus trichocarpa hypothetical protein (POPTR_0001s35240g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa02203
Clone ID Related Accession (Same CDS sequence)	XM_002300472.2
Accession Version	XM_002300472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008467). On Dec 16, 2013 this sequence version replaced XM_002300472.1. COMPLETENESS: incomplete on the 5' end.

ATGGACTCTT ACACTCCTTT ACTCCAGAAA ACACGGGTTC CACAACCCTC GCTCCAAAAA 
TTCGCGGTGA TTTCAATCTT CTCGAAGCTA CGATCCGCGC CGAGTTACCT CGACCCGGAT 
TCCGAACCCG GTAGAGAAGC TATCTCTCAA TGCCTCCGCT CCGCTTCTCC ATCCGTCGTC 
GACCAGTCTG TACGCGAGCT CTGTCGCCTC CTATTGGACT CCAGATTGGA CCTCTCCCGA 
ACTTTGCTGG AGCTCCAGTC TGCACTCGAA GGGTCCGACC CAAAATTTGT TGGTTTATTT 
GTTAAAGCTC TTGGATTTGT CGTTCGGGTC GGGTTTCAAA GGAATCATGG GTCGTGGCGT 
TTTTCTTCTA TTGAGAATCA CCCCTTTGTT ATGATTTTGT CATCAAGAAC TGAGGTTCAG 
TCTGAATTGG TTCAGCAAGT CTTACTATTT TTCGGACAGA ATAGAAGATC GGGAATGGTG 
GAAATTTGTG AATTCTTGAG ACCCTTTTTG AATTTTTGGA TTCTACGCGT GCCCTTTTCG 
AATTCTTCGT CATCTTTGTT TGCAAGGCAG TTGATATCAT CAATGGCTTC GTTTTGTTGT 
TCTTTCCCTG ATGAGGCGAT TCCGGTTTTA AAGCTACTGA TAGGTTGCCT TAAGCATGCG 
TCACATAAGA ATTCAGATGT CATCGATGCA TATACGGTCG TTTTGAGACA CTTGGTTGGA 
ACCGGACTGC TGGTTACTGA GGCTCAACTG TTTGGTGTGG AACTGTCAGA TGCTATTCTT 
TCATTATTGA CATGTCATCA CCGGCATGCT GGTAGTAGTG AACCAATTGT TGAACTAGTG 
AAGCGGCTGT TTGTTGCCCA GAAAAATCTT TCTTTGCAAT ATATGCCTGA AATATCATCA 
ACACTGCTAT CCTTATTTGT TGTCCTGATT CAATCTGATC TTGAATACGA ACAACTCTCT 
TTATTGAAAC TCCTGAACTT CCTTTTAAAA TGGAAAAGTG AAAAGGAATA TGAGGTTGAT 
AGAGTCAAAT GTGCTACGAG CGAAGAGCTT TTGTTCACAT TTCCTGTCAT CAACCTATTA 
TCATCTACAT CTAGATCTAT TAAAGGAGAA GCAGCAGAAT TGCTTGTGAC GCTGGAAAAG 
GTCCTAGTAG AACTGTCAAA AGCACCAAAG GCTGGACTAG CCAAAGAAGG GGGATTTCCC 
CCTATCAGCT CTCTAGGATC GATTGCTTAT AGGCTTTTGC GATGCTTGTG GTTTCAGGAC 
CAGTTTTTAC TGCCCACTTC CTTCCTGAAT TTTGCTTCCA GTGGCAAAAC GGATGTCAAA 
GTAATGCACC AGAAACCAAG ACATTGGGCT TCTCAGTTAA GAGAATACAT TTTGTCGATT 
GTAGACAGAA GAAAATCCTC CCTATCTGTT TCTCAGTCTC AAGAACGTTT CACAAGGGAA 
CTGCCCCCTT TACTCGGTGC CATTACTGGT GTTTTGGTGA TGCACCGGTC ATTTGGAGAT 
ACGGCAATTG ATTTGCTGGG TGCTATTGGC ATTGTGGACC CCAAACAGGG AGTGCCACTG 
CTGCTAGCAA TTCTATTTTA TAGTAACATC TTCACCAGTA AAGACATTAG TTACCAGAAT 
ATGTTGCCAA AACTTCTGGC ATTACTTCCA TCACTTGCTT CACATTCTGT GATGATTCCC 
CTCATAATTC AGACAATCTT GCCGATGCTT CAGAAAGATG GAAAACCTGT GTTGTATGCC 
ACCGGCGCTC GTTTGCTTTG TCAGACCTGG GCAATCAATG ACCGTGCCTT TGGGAGTTTG 
CAAGCAATAT TGCTTCCGAA GGGGCTCACT GAATTCAAGC ATGAGAGAAA CATCTTAATA 
AGTTTGGCTG CCTCCATTCG GGACATTTGC AGAAAGAACC CTGATAGGGG GGTGGATCTT 
ATCTTATCTG TTTCGGCTTG CATTGAGAGC CAAGATCATA TTATTAAAGC TCTTGGATTT 
CAGAGCCTTG CACATCTATG TGAAGCTGAT GTAATTGATT TTTATACTGC CTGGGATGTC 
ATTGGTAAGC ATGCGGTAGA CTACACCACA GACCCGGCTC TTGCACAAAG CATATGCCTC 
CTTCTGAGAT GGGGTGCAAT GGATGCTGAA GCATATTCAG AAGCTTCAAG GAATGTCTTG 
CAAATTTTAT GGGGTATTGG AACTGCTGTA CATGTCAGTC ATGCACTTGA GTGGGCAAGG 
GCAAGGATCT TTGCCTTTGA GGCCCTGAGC CAATATGAGA CAGTGAACAC GGATTTGCTT 
TTACGTGAGA CGAATCTTGA TGTGCTCACG GCTATGGAAG GATTTCAAGT CAAGATCATA 
ACTCATGAAC ATGTGAATCG TCGAAGGTTG GTGAAGGAGA AAAAAATTGC TGGAAGTAAG 
ATTGAGAAGT TGCTAAATGT ATTTCCACAG GTTCTCGTCT CAGGGATAAA AGGTAGTGCT 
GGGCAATTAC CTGGGGCAGC TCTGTTATGC CTTTCCTTTA CCCCTAAAGA TGTGAACAGC 
CAATGCTTGT CAAGAGTATC TGTAGATTTC CATGCTGGAT ATGAGAGTGC ACTGGTGGAA 
ATAGCTGCTT CTCTTCAATT ATCAAGAAAT ATTTTCACTG CCCTTCTTTC ATTGCAATCT 
TGGAAGTCCT TCATGCGGCG TTGGATAAGG GCAAATATTT CATCCCTTGA TGCTAAAGCA 
CCATCTGTTT CATTAGATAA AACTTCTAAA GCTGCTACCG ATATCCTGAA GCGTGTGATG 
CGACTTGCTG AAGAGTCTAT CCCAAGTTCT GCTGAAAATA TTGCTCTGGC TATTGGTGCA 
CTCTGTGTGG TGTTGGCTCC ATCTACCCAT ACTGTCAAAT CAACTGCTTC AAAGTTTCTG 
CTAAACTGGT TGTTCCAGAA TGAGCATGAT CATCGCCAAT GGTCAGCCGC AATATCTCTT 
GGATTGGTTT CAAGTTGTCT TCATGTAACT GATCACAAAC AGAAATTTGA GAATATCACT 
GGACTTATAA AGGTTTTGCA TGGCAGCAAA AGCATCCTCG TCAAAGGAGC TTGTGGGCTT 
GGCTTGGGCT TTGCATGCCA AGACCTTCTT ACCAGGTTTG AAGCTGCTGA TAATGTTGAT 
TTGGATAAAG AAAAGTACAA GGCACAGGAG GTTGACTTAT TAGGGAAGAT TCTTAGGACA 
CTACTTCTGA TGACAAGCCA GCTCAGTAAT GCTTCATATG ATATTCTAGA AAGTCTTCCT 
CCATTTTTCA GCATGGGTGC TAATGATATG GAAATCAATT TGACATCTGA CCAGTTGCTT 
GAGAAGTGTG ATGACTTGGA GGAAGATCCT TGGGGTGTTG CTGGTCTTGT CCTTGGCTTG 
GGGATCTCTT TCAGTGCAAT ATATAGAGCT GGGGCACATG ATGCTATGCT GAAGATAAAG 
GACTTGATTA TATCATGGAT CCCACATGTA AACTCTCTAG TAACGAACTC ATCTTTTTCC 
AGTGAAGGAA GGGAGAAAGC TTTATCAGTA GGATCCTGTC TTGCACTTCC CAGTGTTGTG 
GCATTCTGCC GGAGAGTGGA AATGATCAAT GATAATGAGC TGGATCAACT ATTAAAGGGG 
TATCATGAGC TCATATCTGA GTTATTATCT GTTAAAAAAT CTGGTACTTA CCATCAGAGC 
TTGATGTTGG CATCATGCAT TGGTGCTGGA AGTCTTATCG CTTGCATTTT GAATGAAGGT 
GTGCACCCTC TTGAAGCTGA ATTTGTAAAA GGTTTACTGG AGATGTTTAG AAAATGCTAC 
TGTAGTTCTT TCCCTCCTAT CATCCATTTG GGTGGGATGC TTGGAGTTGT TAATGCAATG 
GGAGCTGGTG CAGGGATTTT GGTTCATGCC CATCACTTCT CAGCCTCAAT CAAGACTGCT 
TGTGAACAAA AGGAATCTTC TCATATTTTG GGCCCTTTAC TTTCAAGCCC TTTTTGTGAG 
CCACATTTGA CAACATTGGT GCAAGAGATA TTTCTCATTG CGCAAAATTC TGATGATCTT 
AAAATGCAAC AAAATGCAGC TTGGGCCGTT TCATTCCTTC GAAATGGTTT GTGGTCAAAG 
GAACTTCTAA ATGCTGAAAG CAATGATCAG ACTGATGTTG TTGATAGCAA AACAATTTCT 
CACAATTTTC CTGAGGACAA TTTAGTTATG AAGCTGACTA TATGGTTAAT GCATCTAAAT 
AACTCCGGGG CAGGGGCTAT TGCACATGTG GGCACTGTTG TAACAGTTTT AAGATGCCTT 
TCCCGAGCTC CTAGATTGCC AACAGTGGAT TGGGGATTGA TCATTAGACG CTGCATGAGA 
TATGAGGCTC AAGTGTCTGA GGTGTTGTTG CCAGATTCAG CTCTTAAGAG GGGAGCCCTT 
CGGGAGGAAT GTGTACAATT CTCAATAGCT CATGCGAATC AATTTGATCC CCTCCTGACT 
TTCCTTGACG AGCTATCTGA TTTGACTAGG TTCAGGACAC TAGAGCTGAA TCTACAGTCA 
TGTCTGCTCT TTCATCTTGC AGGCCTGATA AAAGTATTCT CAGGTTCCAG GCTAGAGAAA 
CTGCTTGATG ATATTGCTGA ATACTTTTGC TCAGATATTT TATACCAAGG GTATAGCTCT 
GATCAGAAGA GCTCATTGAG AATCTCATGC TGGGTGGGTC TCTATCAGTG TCTAGAAGAA 
GCTGTCCTTA GCTCTGTAGA GTACATATCA AACCTTGAAA AGTGCATAGA GGTGCTGTTT 
CACTTGTTGC CTGCATCAGA ATCTGCTGCC TTCACAGGAG TGGATCTGCC AAACGCTGCA 
GAGGAATGGC GTGTGGCAGT TCAATGCTTG GCAAAGGCTC AAGGGGATTG GTTATTGGAT 
TTCCTGCAGG TTCCATTAGG GGATCTTGTG CAAGGAGGTA GCCAGTCAAA TGAAGTCCTA 
AAGAAGATTC TAGCGAAAGT GAAGCTAGTT AGGATGGGTT CCATCCCATT AACTGAGCTT 
GGAAGATTAA AAGCTTATAT GCTGAACTCT AAATCAAAGG ATATTTGGAA TTTGCATGCT 
GAAGTGGTAG CAGCTTTGCA ATATGCAGAT GGTAGTGTCA AAAGACAGTG GCTTGTTGAT 
GCAGTGGAGA TTAGCCTTAA AGTTTCTCGG TCTGCTATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002300508.2
CDS	1..5145
Translation	MDSYTPLLQKTRVPQPSLQKFAVISIFSKLRSAPSYLDPDSEPGREAISQCLRSASPSVVDQSVRELCRLLLDSRLDLSRTLLELQSALEGSDPKFVGLFVKALGFVVRVGFQRNHGSWRFSSIENHPFVMILSSRTEVQSELVQQVLLFFGQNRRSGMVEICEFLRPFLNFWILRVPFSNSSSSLFARQLISSMASFCCSFPDEAIPVLKLLIGCLKHASHKNSDVIDAYTVVLRHLVGTGLLVTEAQLFGVELSDAILSLLTCHHRHAGSSEPIVELVKRLFVAQKNLSLQYMPEISSTLLSLFVVLIQSDLEYEQLSLLKLLNFLLKWKSEKEYEVDRVKCATSEELLFTFPVINLLSSTSRSIKGEAAELLVTLEKVLVELSKAPKAGLAKEGGFPPISSLGSIAYRLLRCLWFQDQFLLPTSFLNFASSGKTDVKVMHQKPRHWASQLREYILSIVDRRKSSLSVSQSQERFTRELPPLLGAITGVLVMHRSFGDTAIDLLGAIGIVDPKQGVPLLLAILFYSNIFTSKDISYQNMLPKLLALLPSLASHSVMIPLIIQTILPMLQKDGKPVLYATGARLLCQTWAINDRAFGSLQAILLPKGLTEFKHERNILISLAASIRDICRKNPDRGVDLILSVSACIESQDHIIKALGFQSLAHLCEADVIDFYTAWDVIGKHAVDYTTDPALAQSICLLLRWGAMDAEAYSEASRNVLQILWGIGTAVHVSHALEWARARIFAFEALSQYETVNTDLLLRETNLDVLTAMEGFQVKIITHEHVNRRRLVKEKKIAGSKIEKLLNVFPQVLVSGIKGSAGQLPGAALLCLSFTPKDVNSQCLSRVSVDFHAGYESALVEIAASLQLSRNIFTALLSLQSWKSFMRRWIRANISSLDAKAPSVSLDKTSKAATDILKRVMRLAEESIPSSAENIALAIGALCVVLAPSTHTVKSTASKFLLNWLFQNEHDHRQWSAAISLGLVSSCLHVTDHKQKFENITGLIKVLHGSKSILVKGACGLGLGFACQDLLTRFEAADNVDLDKEKYKAQEVDLLGKILRTLLLMTSQLSNASYDILESLPPFFSMGANDMEINLTSDQLLEKCDDLEEDPWGVAGLVLGLGISFSAIYRAGAHDAMLKIKDLIISWIPHVNSLVTNSSFSSEGREKALSVGSCLALPSVVAFCRRVEMINDNELDQLLKGYHELISELLSVKKSGTYHQSLMLASCIGAGSLIACILNEGVHPLEAEFVKGLLEMFRKCYCSSFPPIIHLGGMLGVVNAMGAGAGILVHAHHFSASIKTACEQKESSHILGPLLSSPFCEPHLTTLVQEIFLIAQNSDDLKMQQNAAWAVSFLRNGLWSKELLNAESNDQTDVVDSKTISHNFPEDNLVMKLTIWLMHLNNSGAGAIAHVGTVVTVLRCLSRAPRLPTVDWGLIIRRCMRYEAQVSEVLLPDSALKRGALREECVQFSIAHANQFDPLLTFLDELSDLTRFRTLELNLQSCLLFHLAGLIKVFSGSRLEKLLDDIAEYFCSDILYQGYSSDQKSSLRISCWVGLYQCLEEAVLSSVEYISNLEKCIEVLFHLLPASESAAFTGVDLPNAAEEWRVAVQCLAKAQGDWLLDFLQVPLGDLVQGGSQSNEVLKKILAKVKLVRMGSIPLTELGRLKAYMLNSKSKDIWNLHAEVVAALQYADGSVKRQWLVDAVEISLKVSRSAIW

Target ORF information:

RefSeq Version	XM_002300472.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0001s35240g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002300472.2

ORF Insert Sequence:

ATGGACTCTT ACACTCCTTT ACTCCAGAAA ACACGGGTTC CACAACCCTC GCTCCAAAAA 
TTCGCGGTGA TTTCAATCTT CTCGAAGCTA CGATCCGCGC CGAGTTACCT CGACCCGGAT 
TCCGAACCCG GTAGAGAAGC TATCTCTCAA TGCCTCCGCT CCGCTTCTCC ATCCGTCGTC 
GACCAGTCTG TACGCGAGCT CTGTCGCCTC CTATTGGACT CCAGATTGGA CCTCTCCCGA 
ACTTTGCTGG AGCTCCAGTC TGCACTCGAA GGGTCCGACC CAAAATTTGT TGGTTTATTT 
GTTAAAGCTC TTGGATTTGT CGTTCGGGTC GGGTTTCAAA GGAATCATGG GTCGTGGCGT 
TTTTCTTCTA TTGAGAATCA CCCCTTTGTT ATGATTTTGT CATCAAGAAC TGAGGTTCAG 
TCTGAATTGG TTCAGCAAGT CTTACTATTT TTCGGACAGA ATAGAAGATC GGGAATGGTG 
GAAATTTGTG AATTCTTGAG ACCCTTTTTG AATTTTTGGA TTCTACGCGT GCCCTTTTCG 
AATTCTTCGT CATCTTTGTT TGCAAGGCAG TTGATATCAT CAATGGCTTC GTTTTGTTGT 
TCTTTCCCTG ATGAGGCGAT TCCGGTTTTA AAGCTACTGA TAGGTTGCCT TAAGCATGCG 
TCACATAAGA ATTCAGATGT CATCGATGCA TATACGGTCG TTTTGAGACA CTTGGTTGGA 
ACCGGACTGC TGGTTACTGA GGCTCAACTG TTTGGTGTGG AACTGTCAGA TGCTATTCTT 
TCATTATTGA CATGTCATCA CCGGCATGCT GGTAGTAGTG AACCAATTGT TGAACTAGTG 
AAGCGGCTGT TTGTTGCCCA GAAAAATCTT TCTTTGCAAT ATATGCCTGA AATATCATCA 
ACACTGCTAT CCTTATTTGT TGTCCTGATT CAATCTGATC TTGAATACGA ACAACTCTCT 
TTATTGAAAC TCCTGAACTT CCTTTTAAAA TGGAAAAGTG AAAAGGAATA TGAGGTTGAT 
AGAGTCAAAT GTGCTACGAG CGAAGAGCTT TTGTTCACAT TTCCTGTCAT CAACCTATTA 
TCATCTACAT CTAGATCTAT TAAAGGAGAA GCAGCAGAAT TGCTTGTGAC GCTGGAAAAG 
GTCCTAGTAG AACTGTCAAA AGCACCAAAG GCTGGACTAG CCAAAGAAGG GGGATTTCCC 
CCTATCAGCT CTCTAGGATC GATTGCTTAT AGGCTTTTGC GATGCTTGTG GTTTCAGGAC 
CAGTTTTTAC TGCCCACTTC CTTCCTGAAT TTTGCTTCCA GTGGCAAAAC GGATGTCAAA 
GTAATGCACC AGAAACCAAG ACATTGGGCT TCTCAGTTAA GAGAATACAT TTTGTCGATT 
GTAGACAGAA GAAAATCCTC CCTATCTGTT TCTCAGTCTC AAGAACGTTT CACAAGGGAA 
CTGCCCCCTT TACTCGGTGC CATTACTGGT GTTTTGGTGA TGCACCGGTC ATTTGGAGAT 
ACGGCAATTG ATTTGCTGGG TGCTATTGGC ATTGTGGACC CCAAACAGGG AGTGCCACTG 
CTGCTAGCAA TTCTATTTTA TAGTAACATC TTCACCAGTA AAGACATTAG TTACCAGAAT 
ATGTTGCCAA AACTTCTGGC ATTACTTCCA TCACTTGCTT CACATTCTGT GATGATTCCC 
CTCATAATTC AGACAATCTT GCCGATGCTT CAGAAAGATG GAAAACCTGT GTTGTATGCC 
ACCGGCGCTC GTTTGCTTTG TCAGACCTGG GCAATCAATG ACCGTGCCTT TGGGAGTTTG 
CAAGCAATAT TGCTTCCGAA GGGGCTCACT GAATTCAAGC ATGAGAGAAA CATCTTAATA 
AGTTTGGCTG CCTCCATTCG GGACATTTGC AGAAAGAACC CTGATAGGGG GGTGGATCTT 
ATCTTATCTG TTTCGGCTTG CATTGAGAGC CAAGATCATA TTATTAAAGC TCTTGGATTT 
CAGAGCCTTG CACATCTATG TGAAGCTGAT GTAATTGATT TTTATACTGC CTGGGATGTC 
ATTGGTAAGC ATGCGGTAGA CTACACCACA GACCCGGCTC TTGCACAAAG CATATGCCTC 
CTTCTGAGAT GGGGTGCAAT GGATGCTGAA GCATATTCAG AAGCTTCAAG GAATGTCTTG 
CAAATTTTAT GGGGTATTGG AACTGCTGTA CATGTCAGTC ATGCACTTGA GTGGGCAAGG 
GCAAGGATCT TTGCCTTTGA GGCCCTGAGC CAATATGAGA CAGTGAACAC GGATTTGCTT 
TTACGTGAGA CGAATCTTGA TGTGCTCACG GCTATGGAAG GATTTCAAGT CAAGATCATA 
ACTCATGAAC ATGTGAATCG TCGAAGGTTG GTGAAGGAGA AAAAAATTGC TGGAAGTAAG 
ATTGAGAAGT TGCTAAATGT ATTTCCACAG GTTCTCGTCT CAGGGATAAA AGGTAGTGCT 
GGGCAATTAC CTGGGGCAGC TCTGTTATGC CTTTCCTTTA CCCCTAAAGA TGTGAACAGC 
CAATGCTTGT CAAGAGTATC TGTAGATTTC CATGCTGGAT ATGAGAGTGC ACTGGTGGAA 
ATAGCTGCTT CTCTTCAATT ATCAAGAAAT ATTTTCACTG CCCTTCTTTC ATTGCAATCT 
TGGAAGTCCT TCATGCGGCG TTGGATAAGG GCAAATATTT CATCCCTTGA TGCTAAAGCA 
CCATCTGTTT CATTAGATAA AACTTCTAAA GCTGCTACCG ATATCCTGAA GCGTGTGATG 
CGACTTGCTG AAGAGTCTAT CCCAAGTTCT GCTGAAAATA TTGCTCTGGC TATTGGTGCA 
CTCTGTGTGG TGTTGGCTCC ATCTACCCAT ACTGTCAAAT CAACTGCTTC AAAGTTTCTG 
CTAAACTGGT TGTTCCAGAA TGAGCATGAT CATCGCCAAT GGTCAGCCGC AATATCTCTT 
GGATTGGTTT CAAGTTGTCT TCATGTAACT GATCACAAAC AGAAATTTGA GAATATCACT 
GGACTTATAA AGGTTTTGCA TGGCAGCAAA AGCATCCTCG TCAAAGGAGC TTGTGGGCTT 
GGCTTGGGCT TTGCATGCCA AGACCTTCTT ACCAGGTTTG AAGCTGCTGA TAATGTTGAT 
TTGGATAAAG AAAAGTACAA GGCACAGGAG GTTGACTTAT TAGGGAAGAT TCTTAGGACA 
CTACTTCTGA TGACAAGCCA GCTCAGTAAT GCTTCATATG ATATTCTAGA AAGTCTTCCT 
CCATTTTTCA GCATGGGTGC TAATGATATG GAAATCAATT TGACATCTGA CCAGTTGCTT 
GAGAAGTGTG ATGACTTGGA GGAAGATCCT TGGGGTGTTG CTGGTCTTGT CCTTGGCTTG 
GGGATCTCTT TCAGTGCAAT ATATAGAGCT GGGGCACATG ATGCTATGCT GAAGATAAAG 
GACTTGATTA TATCATGGAT CCCACATGTA AACTCTCTAG TAACGAACTC ATCTTTTTCC 
AGTGAAGGAA GGGAGAAAGC TTTATCAGTA GGATCCTGTC TTGCACTTCC CAGTGTTGTG 
GCATTCTGCC GGAGAGTGGA AATGATCAAT GATAATGAGC TGGATCAACT ATTAAAGGGG 
TATCATGAGC TCATATCTGA GTTATTATCT GTTAAAAAAT CTGGTACTTA CCATCAGAGC 
TTGATGTTGG CATCATGCAT TGGTGCTGGA AGTCTTATCG CTTGCATTTT GAATGAAGGT 
GTGCACCCTC TTGAAGCTGA ATTTGTAAAA GGTTTACTGG AGATGTTTAG AAAATGCTAC 
TGTAGTTCTT TCCCTCCTAT CATCCATTTG GGTGGGATGC TTGGAGTTGT TAATGCAATG 
GGAGCTGGTG CAGGGATTTT GGTTCATGCC CATCACTTCT CAGCCTCAAT CAAGACTGCT 
TGTGAACAAA AGGAATCTTC TCATATTTTG GGCCCTTTAC TTTCAAGCCC TTTTTGTGAG 
CCACATTTGA CAACATTGGT GCAAGAGATA TTTCTCATTG CGCAAAATTC TGATGATCTT 
AAAATGCAAC AAAATGCAGC TTGGGCCGTT TCATTCCTTC GAAATGGTTT GTGGTCAAAG 
GAACTTCTAA ATGCTGAAAG CAATGATCAG ACTGATGTTG TTGATAGCAA AACAATTTCT 
CACAATTTTC CTGAGGACAA TTTAGTTATG AAGCTGACTA TATGGTTAAT GCATCTAAAT 
AACTCCGGGG CAGGGGCTAT TGCACATGTG GGCACTGTTG TAACAGTTTT AAGATGCCTT 
TCCCGAGCTC CTAGATTGCC AACAGTGGAT TGGGGATTGA TCATTAGACG CTGCATGAGA 
TATGAGGCTC AAGTGTCTGA GGTGTTGTTG CCAGATTCAG CTCTTAAGAG GGGAGCCCTT 
CGGGAGGAAT GTGTACAATT CTCAATAGCT CATGCGAATC AATTTGATCC CCTCCTGACT 
TTCCTTGACG AGCTATCTGA TTTGACTAGG TTCAGGACAC TAGAGCTGAA TCTACAGTCA 
TGTCTGCTCT TTCATCTTGC AGGCCTGATA AAAGTATTCT CAGGTTCCAG GCTAGAGAAA 
CTGCTTGATG ATATTGCTGA ATACTTTTGC TCAGATATTT TATACCAAGG GTATAGCTCT 
GATCAGAAGA GCTCATTGAG AATCTCATGC TGGGTGGGTC TCTATCAGTG TCTAGAAGAA 
GCTGTCCTTA GCTCTGTAGA GTACATATCA AACCTTGAAA AGTGCATAGA GGTGCTGTTT 
CACTTGTTGC CTGCATCAGA ATCTGCTGCC TTCACAGGAG TGGATCTGCC AAACGCTGCA 
GAGGAATGGC GTGTGGCAGT TCAATGCTTG GCAAAGGCTC AAGGGGATTG GTTATTGGAT 
TTCCTGCAGG TTCCATTAGG GGATCTTGTG CAAGGAGGTA GCCAGTCAAA TGAAGTCCTA 
AAGAAGATTC TAGCGAAAGT GAAGCTAGTT AGGATGGGTT CCATCCCATT AACTGAGCTT 
GGAAGATTAA AAGCTTATAT GCTGAACTCT AAATCAAAGG ATATTTGGAA TTTGCATGCT 
GAAGTGGTAG CAGCTTTGCA ATATGCAGAT GGTAGTGTCA AAAGACAGTG GCTTGTTGAT 
GCAGTGGAGA TTAGCCTTAA AGTTTCTCGG TCTGCTATCT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0001s35240g ( XM_002300012.2 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_002300048.2 Populus trichocarpa hypothetical protein (POPTR_0001s35240g) mRNA, complete cds.

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CloneID	OPa02204
Clone ID Related Accession (Same CDS sequence)	XM_002300012.2
Accession Version	XM_002300012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008467). On Dec 16, 2013 this sequence version replaced XM_002300012.1. COMPLETENESS: incomplete on the 5' end.

ATGGACTCTT ACACTCCTTT ACTCCAGAAA ACACGGGTTC CACAACCCTC GCTCCAAAAA 
TTCGCGGTGA TTTCAATCTT CTCGAAGCTA CGATCCGCGC CGAGTTACCT CGACCCGGAT 
TCCGAACCCG GTAGAGAAGC TATCTCTCAA TGCCTCCGCT CCGCTTCTCC ATCCGTCGTC 
GACCAGTCTG TACGCGAGCT CTGTCGCCTC CTATTGGACT CCAGATTGGA CCTCTCCCGA 
ACTTTGCTGG AGCTCCAGTC TGCACTCGAA GGGTCCGACC CAAAATTTGT TGGTTTATTT 
GTTAAAGCTC TTGGATTTGT CGTTCGGGTC GGGTTTCAAA GGAATCATGG GTCGTGGCGT 
TTTTCTTCTA TTGAGAATCA CCCCTTTGTT ATGATTTTGT CATCAAGAAC TGAGGTTCAG 
TCTGAATTGG TTCAGCAAGT CTTACTATTT TTCGGACAGA ATAGAAGATC GGGAATGGTG 
GAAATTTGTG AATTCTTGAG ACCCTTTTTG AATTTTTGGA TTCTACGCGT GCCCTTTTCG 
AATTCTTCGT CATCTTTGTT TGCAAGGCAG TTGATATCAT CAATGGCTTC GTTTTGTTGT 
TCTTTCCCTG ATGAGGCGAT TCCGGTTTTA AAGCTACTGA TAGGTTGCCT TAAGCATGCG 
TCACATAAGA ATTCAGATGT CATCGATGCA TATACGGTCG TTTTGAGACA CTTGGTTGGA 
ACCGGACTGC TGGTTACTGA GGCTCAACTG TTTGGTGTGG AACTGTCAGA TGCTATTCTT 
TCATTATTGA CATGTCATCA CCGGCATGCT GGTAGTAGTG AACCAATTGT TGAACTAGTG 
AAGCGGCTGT TTGTTGCCCA GAAAAATCTT TCTTTGCAAT ATATGCCTGA AATATCATCA 
ACACTGCTAT CCTTATTTGT TGTCCTGATT CAATCTGATC TTGAATACGA ACAACTCTCT 
TTATTGAAAC TCCTGAACTT CCTTTTAAAA TGGAAAAGTG AAAAGGAATA TGAGGTTGAT 
AGAGTCAAAT GTGCTACGAG CGAAGAGCTT TTGTTCACAT TTCCTGTCAT CAACCTATTA 
TCATCTACAT CTAGATCTAT TAAAGGAGAA GCAGCAGAAT TGCTTGTGAC GCTGGAAAAG 
GTCCTAGTAG AACTGTCAAA AGCACCAAAG GCTGGACTAG CCAAAGAAGG GGGATTTCCC 
CCTATCAGCT CTCTAGGATC GATTGCTTAT AGGCTTTTGC GATGCTTGTG GTTTCAGGAC 
CAGTTTTTAC TGCCCACTTC CTTCCTGAAT TTTGCTTCCA GTGGCAAAAC GGATGTCAAA 
GTAATGCACC AGAAACCAAG ACATTGGGCT TCTCAGTTAA GAGAATACAT TTTGTCGATT 
GTAGACAGAA GAAAATCCTC CCTATCTGTT TCTCAGTCTC AAGAACGTTT CACAAGGGAA 
CTGCCCCCTT TACTCGGTGC CATTACTGGT GTTTTGGTGA TGCACCGGTC ATTTGGAGAT 
ACGGCAATTG ATTTGCTGGG TGCTATTGGC ATTGTGGACC CCAAACAGGG AGTGCCACTG 
CTGCTAGCAA TTCTATTTTA TAGTAACATC TTCACCAGTA AAGACATTAG TTACCAGAAT 
ATGTTGCCAA AACTTCTGGC ATTACTTCCA TCACTTGCTT CACATTCTGT GATGATTCCC 
CTCATAATTC AGACAATCTT GCCGATGCTT CAGAAAGATG GAAAACCTGT GTTGTATGCC 
ACCGGCGCTC GTTTGCTTTG TCAGACCTGG GCAATCAATG ACCGTGCCTT TGGGAGTTTG 
CAAGCAATAT TGCTTCCGAA GGGGCTCACT GAATTCAAGC ATGAGAGAAA CATCTTAATA 
AGTTTGGCTG CCTCCATTCG GGACATTTGC AGAAAGAACC CTGATAGGGG GGTGGATCTT 
ATCTTATCTG TTTCGGCTTG CATTGAGAGC CAAGATCATA TTATTAAAGC TCTTGGATTT 
CAGAGCCTTG CACATCTATG TGAAGCTGAT GTAATTGATT TTTATACTGC CTGGGATGTC 
ATTGGTAAGC ATGCGGTAGA CTACACCACA GACCCGGCTC TTGCACAAAG CATATGCCTC 
CTTCTGAGAT GGGGTGCAAT GGATGCTGAA GCATATTCAG AAGCTTCAAG GAATGTCTTG 
CAAATTTTAT GGGGTATTGG AACTGCTGTA CATGTCAGTC ATGCACTTGA GTGGGCAAGG 
GCAAGGATCT TTGCCTTTGA GGCCCTGAGC CAATATGAGA CAGTGAACAC GGATTTGCTT 
TTACGTGAGA CGAATCTTGA TGTGCTCACG GCTATGGAAG GATTTCAAGT CAAGATCATA 
ACTCATGAAC ATGTGAATCG TCGAAGGTTG GTGAAGGAGA AAAAAATTGC TGGAAGTAAG 
ATTGAGAAGT TGCTAAATGT ATTTCCACAG GTTCTCGTCT CAGGGATAAA AGGTAGTGCT 
GGGCAATTAC CTGGGGCAGC TCTGTTATGC CTTTCCTTTA CCCCTAAAGA TGTGAACAGC 
CAATGCTTGT CAAGAGTATC TGTAGATTTC CATGCTGGAT ATGAGAGTGC ACTGGTGGAA 
ATAGCTGCTT CTCTTCAATT ATCAAGAAAT ATTTTCACTG CCCTTCTTTC ATTGCAATCT 
TGGAAGTCCT TCATGCGGCG TTGGATAAGG GCAAATATTT CATCCCTTGA TGCTAAAGCA 
CCATCTGTTT CATTAGATAA AACTTCTAAA GCTGCTACCG ATATCCTGAA GCGTGTGATG 
CGACTTGCTG AAGAGTCTAT CCCAAGTTCT GCTGAAAATA TTGCTCTGGC TATTGGTGCA 
CTCTGTGTGG TGTTGGCTCC ATCTACCCAT ACTGTCAAAT CAACTGCTTC AAAGTTTCTG 
CTAAACTGGT TGTTCCAGAA TGAGCATGAT CATCGCCAAT GGTCAGCCGC AATATCTCTT 
GGATTGGTTT CAAGTTGTCT TCATGTAACT GATCACAAAC AGAAATTTGA GAATATCACT 
GGACTTATAA AGGTTTTGCA TGGCAGCAAA AGCATCCTCG TCAAAGGAGC TTGTGGGCTT 
GGCTTGGGCT TTGCATGCCA AGACCTTCTT ACCAGGTTTG AAGCTGCTGA TAATGTTGAT 
TTGGATAAAG AAAAGTACAA GGCACAGGAG GTTGACTTAT TAGGGAAGAT TCTTAGGACA 
CTACTTCTGA TGACAAGCCA GCTCAGTAAT GCTTCATATG ATATTCTAGA AAGTCTTCCT 
CCATTTTTCA GCATGGGTGC TAATGATATG GAAATCAATT TGACATCTGA CCAGTTGCTT 
GAGAAGTGTG ATGACTTGGA GGAAGATCCT TGGGGTGTTG CTGGTCTTGT CCTTGGCTTG 
GGGATCTCTT TCAGTGCAAT ATATAGAGCT GGGGCACATG ATGCTATGCT GAAGATAAAG 
GACTTGATTA TATCATGGAT CCCACATGTA AACTCTCTAG TAACGAACTC ATCTTTTTCC 
AGTGAAGGAA GGGAGAAAGC TTTATCAGTA GGATCCTGTC TTGCACTTCC CAGTGTTGTG 
GCATTCTGCC GGAGAGTGGA AATGATCAAT GATAATGAGC TGGATCAACT ATTAAAGGGG 
TATCATGAGC TCATATCTGA GTTATTATCT GTTAAAAAAT CTGGTACTTA CCATCAGAGC 
TTGATGTTGG CATCATGCAT TGGTGCTGGA AGTCTTATCG CTTGCATTTT GAATGAAGGT 
GTGCACCCTC TTGAAGCTGA ATTTGTAAAA GGTTTACTGG AGATGTTTAG AAAATGCTAC 
TGTAGTTCTT TCCCTCCTAT CATCCATTTG GGTGGGATGC TTGGAGTTGT TAATGCAATG 
GGAGCTGGTG CAGGGATTTT GGTTCATGCC CATCACTTCT CAGCCTCAAT CAAGACTGCT 
TGTGAACAAA AGGAATCTTC TCATATTTTG GGCCCTTTAC TTTCAAGCCC TTTTTGTGAG 
CCACATTTGA CAACATTGGT GCAAGAGATA TTTCTCATTG CGCAAAATTC TGATGATCTT 
AAAATGCAAC AAAATGCAGC TTGGGCCGTT TCATTCCTTC GAAATGGTTT GTGGTCAAAG 
GAACTTCTAA ATGCTGAAAG CAATGATCAG ACTGATGTTG TTGATAGCAA AACAATTTCT 
CACAATTTTC CTGAGGACAA TTTAGTTATG AAGCTGACTA TATGGTTAAT GCATCTAAAT 
AACTCCGGGG CAGGGGCTAT TGCACATGTG GGCACTGTTG TAACAGTTTT AAGATGCCTT 
TCCCGAGCTC CTAGATTGCC AACAGTGGAT TGGGGATTGA TCATTAGACG CTGCATGAGA 
TATGAGGCTC AAGTGTCTGA GGTGTTGTTG CCAGATTCAG CTCTTAAGAG GGGAGCCCTT 
CGGGAGGAAT GTGTACAATT CTCAATAGCT CATGCGAATC AATTTGATCC CCTCCTGACT 
TTCCTTGACG AGCTATCTGA TTTGACTAGG TTCAGGACAC TAGAGCTGAA TCTACAGTCA 
TGTCTGCTCT TTCATCTTGC AGGCCTGATA AAAGTATTCT CAGGTTCCAG GCTAGAGAAA 
CTGCTTGATG ATATTGCTGA ATACTTTTGC TCAGATATTT TATACCAAGG GTATAGCTCT 
GATCAGAAGA GCTCATTGAG AATCTCATGC TGGGTGGGTC TCTATCAGTG TCTAGAAGAA 
GCTGTCCTTA GCTCTGTAGA GTACATATCA AACCTTGAAA AGTGCATAGA GGTGCTGTTT 
CACTTGTTGC CTGCATCAGA ATCTGCTGCC TTCACAGGAG TGGATCTGCC AAACGCTGCA 
GAGGAATGGC GTGTGGCAGT TCAATGCTTG GCAAAGGCTC AAGGGGATTG GTTATTGGAT 
TTCCTGCAGG TTCCATTAGG GGATCTTGTG CAAGGAGGTA GCCAGTCAAA TGAAGTCCTA 
AAGAAGATTC TAGCGAAAGT GAAGCTAGTT AGGATGGGTT CCATCCCATT AACTGAGCTT 
GGAAGATTAA AAGCTTATAT GCTGAACTCT AAATCAAAGG ATATTTGGAA TTTGCATGCT 
GAAGTGGTAG CAGCTTTGCA ATATGCAGAT GGTAGTGTCA AAAGACAGTG GCTTGTTGAT 
GCAGTGGAGA TTAGTTGTGT CTCGAGCTAT CCTTCCATAG CCTTAAAGTT TCTCGGTCTG 
CTATCTGGTA GCTGTTGCAA ATATGGGTCT CTTCTGACTT TGGACCAGCT ATCCGTGTTG 
AGTGATCTAC CAGTTACCCT CCCTTCCCTT GTGACCGAAC CTAGTTGGGA AGTTGTTGCG 
GAATCCATTG TTTCTACCCT TTGGACATCA ACCGAACGCA TATACTACTT GGTTACTGAC 
AAAGGACCCC CTGACAATAC AAATAGCACG CAACCTATTG ACGGAAGTGA AAAGGATATT 
GCCTCCTTTC TATTGCATGT GATGTATCAT ACCTGTACAT GTTTAAAAGA GTATTTGCCT 
TTGGAGAAGC AGCTTAGGCT TGCCAATATG CTGGTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002300048.2
CDS	1..5502
Translation	MDSYTPLLQKTRVPQPSLQKFAVISIFSKLRSAPSYLDPDSEPGREAISQCLRSASPSVVDQSVRELCRLLLDSRLDLSRTLLELQSALEGSDPKFVGLFVKALGFVVRVGFQRNHGSWRFSSIENHPFVMILSSRTEVQSELVQQVLLFFGQNRRSGMVEICEFLRPFLNFWILRVPFSNSSSSLFARQLISSMASFCCSFPDEAIPVLKLLIGCLKHASHKNSDVIDAYTVVLRHLVGTGLLVTEAQLFGVELSDAILSLLTCHHRHAGSSEPIVELVKRLFVAQKNLSLQYMPEISSTLLSLFVVLIQSDLEYEQLSLLKLLNFLLKWKSEKEYEVDRVKCATSEELLFTFPVINLLSSTSRSIKGEAAELLVTLEKVLVELSKAPKAGLAKEGGFPPISSLGSIAYRLLRCLWFQDQFLLPTSFLNFASSGKTDVKVMHQKPRHWASQLREYILSIVDRRKSSLSVSQSQERFTRELPPLLGAITGVLVMHRSFGDTAIDLLGAIGIVDPKQGVPLLLAILFYSNIFTSKDISYQNMLPKLLALLPSLASHSVMIPLIIQTILPMLQKDGKPVLYATGARLLCQTWAINDRAFGSLQAILLPKGLTEFKHERNILISLAASIRDICRKNPDRGVDLILSVSACIESQDHIIKALGFQSLAHLCEADVIDFYTAWDVIGKHAVDYTTDPALAQSICLLLRWGAMDAEAYSEASRNVLQILWGIGTAVHVSHALEWARARIFAFEALSQYETVNTDLLLRETNLDVLTAMEGFQVKIITHEHVNRRRLVKEKKIAGSKIEKLLNVFPQVLVSGIKGSAGQLPGAALLCLSFTPKDVNSQCLSRVSVDFHAGYESALVEIAASLQLSRNIFTALLSLQSWKSFMRRWIRANISSLDAKAPSVSLDKTSKAATDILKRVMRLAEESIPSSAENIALAIGALCVVLAPSTHTVKSTASKFLLNWLFQNEHDHRQWSAAISLGLVSSCLHVTDHKQKFENITGLIKVLHGSKSILVKGACGLGLGFACQDLLTRFEAADNVDLDKEKYKAQEVDLLGKILRTLLLMTSQLSNASYDILESLPPFFSMGANDMEINLTSDQLLEKCDDLEEDPWGVAGLVLGLGISFSAIYRAGAHDAMLKIKDLIISWIPHVNSLVTNSSFSSEGREKALSVGSCLALPSVVAFCRRVEMINDNELDQLLKGYHELISELLSVKKSGTYHQSLMLASCIGAGSLIACILNEGVHPLEAEFVKGLLEMFRKCYCSSFPPIIHLGGMLGVVNAMGAGAGILVHAHHFSASIKTACEQKESSHILGPLLSSPFCEPHLTTLVQEIFLIAQNSDDLKMQQNAAWAVSFLRNGLWSKELLNAESNDQTDVVDSKTISHNFPEDNLVMKLTIWLMHLNNSGAGAIAHVGTVVTVLRCLSRAPRLPTVDWGLIIRRCMRYEAQVSEVLLPDSALKRGALREECVQFSIAHANQFDPLLTFLDELSDLTRFRTLELNLQSCLLFHLAGLIKVFSGSRLEKLLDDIAEYFCSDILYQGYSSDQKSSLRISCWVGLYQCLEEAVLSSVEYISNLEKCIEVLFHLLPASESAAFTGVDLPNAAEEWRVAVQCLAKAQGDWLLDFLQVPLGDLVQGGSQSNEVLKKILAKVKLVRMGSIPLTELGRLKAYMLNSKSKDIWNLHAEVVAALQYADGSVKRQWLVDAVEISCVSSYPSIALKFLGLLSGSCCKYGSLLTLDQLSVLSDLPVTLPSLVTEPSWEVVAESIVSTLWTSTERIYYLVTDKGPPDNTNSTQPIDGSEKDIASFLLHVMYHTCTCLKEYLPLEKQLRLANMLVT

Target ORF information:

RefSeq Version	XM_002300012.2
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0001s35240g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002300012.2

ORF Insert Sequence:

ATGGACTCTT ACACTCCTTT ACTCCAGAAA ACACGGGTTC CACAACCCTC GCTCCAAAAA 
TTCGCGGTGA TTTCAATCTT CTCGAAGCTA CGATCCGCGC CGAGTTACCT CGACCCGGAT 
TCCGAACCCG GTAGAGAAGC TATCTCTCAA TGCCTCCGCT CCGCTTCTCC ATCCGTCGTC 
GACCAGTCTG TACGCGAGCT CTGTCGCCTC CTATTGGACT CCAGATTGGA CCTCTCCCGA 
ACTTTGCTGG AGCTCCAGTC TGCACTCGAA GGGTCCGACC CAAAATTTGT TGGTTTATTT 
GTTAAAGCTC TTGGATTTGT CGTTCGGGTC GGGTTTCAAA GGAATCATGG GTCGTGGCGT 
TTTTCTTCTA TTGAGAATCA CCCCTTTGTT ATGATTTTGT CATCAAGAAC TGAGGTTCAG 
TCTGAATTGG TTCAGCAAGT CTTACTATTT TTCGGACAGA ATAGAAGATC GGGAATGGTG 
GAAATTTGTG AATTCTTGAG ACCCTTTTTG AATTTTTGGA TTCTACGCGT GCCCTTTTCG 
AATTCTTCGT CATCTTTGTT TGCAAGGCAG TTGATATCAT CAATGGCTTC GTTTTGTTGT 
TCTTTCCCTG ATGAGGCGAT TCCGGTTTTA AAGCTACTGA TAGGTTGCCT TAAGCATGCG 
TCACATAAGA ATTCAGATGT CATCGATGCA TATACGGTCG TTTTGAGACA CTTGGTTGGA 
ACCGGACTGC TGGTTACTGA GGCTCAACTG TTTGGTGTGG AACTGTCAGA TGCTATTCTT 
TCATTATTGA CATGTCATCA CCGGCATGCT GGTAGTAGTG AACCAATTGT TGAACTAGTG 
AAGCGGCTGT TTGTTGCCCA GAAAAATCTT TCTTTGCAAT ATATGCCTGA AATATCATCA 
ACACTGCTAT CCTTATTTGT TGTCCTGATT CAATCTGATC TTGAATACGA ACAACTCTCT 
TTATTGAAAC TCCTGAACTT CCTTTTAAAA TGGAAAAGTG AAAAGGAATA TGAGGTTGAT 
AGAGTCAAAT GTGCTACGAG CGAAGAGCTT TTGTTCACAT TTCCTGTCAT CAACCTATTA 
TCATCTACAT CTAGATCTAT TAAAGGAGAA GCAGCAGAAT TGCTTGTGAC GCTGGAAAAG 
GTCCTAGTAG AACTGTCAAA AGCACCAAAG GCTGGACTAG CCAAAGAAGG GGGATTTCCC 
CCTATCAGCT CTCTAGGATC GATTGCTTAT AGGCTTTTGC GATGCTTGTG GTTTCAGGAC 
CAGTTTTTAC TGCCCACTTC CTTCCTGAAT TTTGCTTCCA GTGGCAAAAC GGATGTCAAA 
GTAATGCACC AGAAACCAAG ACATTGGGCT TCTCAGTTAA GAGAATACAT TTTGTCGATT 
GTAGACAGAA GAAAATCCTC CCTATCTGTT TCTCAGTCTC AAGAACGTTT CACAAGGGAA 
CTGCCCCCTT TACTCGGTGC CATTACTGGT GTTTTGGTGA TGCACCGGTC ATTTGGAGAT 
ACGGCAATTG ATTTGCTGGG TGCTATTGGC ATTGTGGACC CCAAACAGGG AGTGCCACTG 
CTGCTAGCAA TTCTATTTTA TAGTAACATC TTCACCAGTA AAGACATTAG TTACCAGAAT 
ATGTTGCCAA AACTTCTGGC ATTACTTCCA TCACTTGCTT CACATTCTGT GATGATTCCC 
CTCATAATTC AGACAATCTT GCCGATGCTT CAGAAAGATG GAAAACCTGT GTTGTATGCC 
ACCGGCGCTC GTTTGCTTTG TCAGACCTGG GCAATCAATG ACCGTGCCTT TGGGAGTTTG 
CAAGCAATAT TGCTTCCGAA GGGGCTCACT GAATTCAAGC ATGAGAGAAA CATCTTAATA 
AGTTTGGCTG CCTCCATTCG GGACATTTGC AGAAAGAACC CTGATAGGGG GGTGGATCTT 
ATCTTATCTG TTTCGGCTTG CATTGAGAGC CAAGATCATA TTATTAAAGC TCTTGGATTT 
CAGAGCCTTG CACATCTATG TGAAGCTGAT GTAATTGATT TTTATACTGC CTGGGATGTC 
ATTGGTAAGC ATGCGGTAGA CTACACCACA GACCCGGCTC TTGCACAAAG CATATGCCTC 
CTTCTGAGAT GGGGTGCAAT GGATGCTGAA GCATATTCAG AAGCTTCAAG GAATGTCTTG 
CAAATTTTAT GGGGTATTGG AACTGCTGTA CATGTCAGTC ATGCACTTGA GTGGGCAAGG 
GCAAGGATCT TTGCCTTTGA GGCCCTGAGC CAATATGAGA CAGTGAACAC GGATTTGCTT 
TTACGTGAGA CGAATCTTGA TGTGCTCACG GCTATGGAAG GATTTCAAGT CAAGATCATA 
ACTCATGAAC ATGTGAATCG TCGAAGGTTG GTGAAGGAGA AAAAAATTGC TGGAAGTAAG 
ATTGAGAAGT TGCTAAATGT ATTTCCACAG GTTCTCGTCT CAGGGATAAA AGGTAGTGCT 
GGGCAATTAC CTGGGGCAGC TCTGTTATGC CTTTCCTTTA CCCCTAAAGA TGTGAACAGC 
CAATGCTTGT CAAGAGTATC TGTAGATTTC CATGCTGGAT ATGAGAGTGC ACTGGTGGAA 
ATAGCTGCTT CTCTTCAATT ATCAAGAAAT ATTTTCACTG CCCTTCTTTC ATTGCAATCT 
TGGAAGTCCT TCATGCGGCG TTGGATAAGG GCAAATATTT CATCCCTTGA TGCTAAAGCA 
CCATCTGTTT CATTAGATAA AACTTCTAAA GCTGCTACCG ATATCCTGAA GCGTGTGATG 
CGACTTGCTG AAGAGTCTAT CCCAAGTTCT GCTGAAAATA TTGCTCTGGC TATTGGTGCA 
CTCTGTGTGG TGTTGGCTCC ATCTACCCAT ACTGTCAAAT CAACTGCTTC AAAGTTTCTG 
CTAAACTGGT TGTTCCAGAA TGAGCATGAT CATCGCCAAT GGTCAGCCGC AATATCTCTT 
GGATTGGTTT CAAGTTGTCT TCATGTAACT GATCACAAAC AGAAATTTGA GAATATCACT 
GGACTTATAA AGGTTTTGCA TGGCAGCAAA AGCATCCTCG TCAAAGGAGC TTGTGGGCTT 
GGCTTGGGCT TTGCATGCCA AGACCTTCTT ACCAGGTTTG AAGCTGCTGA TAATGTTGAT 
TTGGATAAAG AAAAGTACAA GGCACAGGAG GTTGACTTAT TAGGGAAGAT TCTTAGGACA 
CTACTTCTGA TGACAAGCCA GCTCAGTAAT GCTTCATATG ATATTCTAGA AAGTCTTCCT 
CCATTTTTCA GCATGGGTGC TAATGATATG GAAATCAATT TGACATCTGA CCAGTTGCTT 
GAGAAGTGTG ATGACTTGGA GGAAGATCCT TGGGGTGTTG CTGGTCTTGT CCTTGGCTTG 
GGGATCTCTT TCAGTGCAAT ATATAGAGCT GGGGCACATG ATGCTATGCT GAAGATAAAG 
GACTTGATTA TATCATGGAT CCCACATGTA AACTCTCTAG TAACGAACTC ATCTTTTTCC 
AGTGAAGGAA GGGAGAAAGC TTTATCAGTA GGATCCTGTC TTGCACTTCC CAGTGTTGTG 
GCATTCTGCC GGAGAGTGGA AATGATCAAT GATAATGAGC TGGATCAACT ATTAAAGGGG 
TATCATGAGC TCATATCTGA GTTATTATCT GTTAAAAAAT CTGGTACTTA CCATCAGAGC 
TTGATGTTGG CATCATGCAT TGGTGCTGGA AGTCTTATCG CTTGCATTTT GAATGAAGGT 
GTGCACCCTC TTGAAGCTGA ATTTGTAAAA GGTTTACTGG AGATGTTTAG AAAATGCTAC 
TGTAGTTCTT TCCCTCCTAT CATCCATTTG GGTGGGATGC TTGGAGTTGT TAATGCAATG 
GGAGCTGGTG CAGGGATTTT GGTTCATGCC CATCACTTCT CAGCCTCAAT CAAGACTGCT 
TGTGAACAAA AGGAATCTTC TCATATTTTG GGCCCTTTAC TTTCAAGCCC TTTTTGTGAG 
CCACATTTGA CAACATTGGT GCAAGAGATA TTTCTCATTG CGCAAAATTC TGATGATCTT 
AAAATGCAAC AAAATGCAGC TTGGGCCGTT TCATTCCTTC GAAATGGTTT GTGGTCAAAG 
GAACTTCTAA ATGCTGAAAG CAATGATCAG ACTGATGTTG TTGATAGCAA AACAATTTCT 
CACAATTTTC CTGAGGACAA TTTAGTTATG AAGCTGACTA TATGGTTAAT GCATCTAAAT 
AACTCCGGGG CAGGGGCTAT TGCACATGTG GGCACTGTTG TAACAGTTTT AAGATGCCTT 
TCCCGAGCTC CTAGATTGCC AACAGTGGAT TGGGGATTGA TCATTAGACG CTGCATGAGA 
TATGAGGCTC AAGTGTCTGA GGTGTTGTTG CCAGATTCAG CTCTTAAGAG GGGAGCCCTT 
CGGGAGGAAT GTGTACAATT CTCAATAGCT CATGCGAATC AATTTGATCC CCTCCTGACT 
TTCCTTGACG AGCTATCTGA TTTGACTAGG TTCAGGACAC TAGAGCTGAA TCTACAGTCA 
TGTCTGCTCT TTCATCTTGC AGGCCTGATA AAAGTATTCT CAGGTTCCAG GCTAGAGAAA 
CTGCTTGATG ATATTGCTGA ATACTTTTGC TCAGATATTT TATACCAAGG GTATAGCTCT 
GATCAGAAGA GCTCATTGAG AATCTCATGC TGGGTGGGTC TCTATCAGTG TCTAGAAGAA 
GCTGTCCTTA GCTCTGTAGA GTACATATCA AACCTTGAAA AGTGCATAGA GGTGCTGTTT 
CACTTGTTGC CTGCATCAGA ATCTGCTGCC TTCACAGGAG TGGATCTGCC AAACGCTGCA 
GAGGAATGGC GTGTGGCAGT TCAATGCTTG GCAAAGGCTC AAGGGGATTG GTTATTGGAT 
TTCCTGCAGG TTCCATTAGG GGATCTTGTG CAAGGAGGTA GCCAGTCAAA TGAAGTCCTA 
AAGAAGATTC TAGCGAAAGT GAAGCTAGTT AGGATGGGTT CCATCCCATT AACTGAGCTT 
GGAAGATTAA AAGCTTATAT GCTGAACTCT AAATCAAAGG ATATTTGGAA TTTGCATGCT 
GAAGTGGTAG CAGCTTTGCA ATATGCAGAT GGTAGTGTCA AAAGACAGTG GCTTGTTGAT 
GCAGTGGAGA TTAGTTGTGT CTCGAGCTAT CCTTCCATAG CCTTAAAGTT TCTCGGTCTG 
CTATCTGGTA GCTGTTGCAA ATATGGGTCT CTTCTGACTT TGGACCAGCT ATCCGTGTTG 
AGTGATCTAC CAGTTACCCT CCCTTCCCTT GTGACCGAAC CTAGTTGGGA AGTTGTTGCG 
GAATCCATTG TTTCTACCCT TTGGACATCA ACCGAACGCA TATACTACTT GGTTACTGAC 
AAAGGACCCC CTGACAATAC AAATAGCACG CAACCTATTG ACGGAAGTGA AAAGGATATT 
GCCTCCTTTC TATTGCATGT GATGTATCAT ACCTGTACAT GTTTAAAAGA GTATTTGCCT 
TTGGAGAAGC AGCTTAGGCT TGCCAATATG CTGGTCACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0001s35240g ( XM_024609538.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024465306.1 Populus trichocarpa protein RST1 (LOC7490707), transcript variant X1, mRNA.

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CloneID	OPa421716
Clone ID Related Accession (Same CDS sequence)	XM_024609538.1
Accession Version	XM_024609538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	protein RST1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTT ACACTCCTTT ACTCCAGAAA ACACGGGTTC CACAACCCTC GCTCCAAAAA 
TTCGCGGTGA TTTCAATCTT CTCGAAGCTA CGATCCGCGC CGAGTTACCT CGACCCGGAT 
TCCGAACCCG GTAGAGAAGC TATCTCTCAA TGCCTCCGCT CCGCTTCTCC ATCCGTCGTC 
GACCAGTCTG TACGCGAGCT CTGTCGCCTC CTATTGGACT CCAGATTGGA CCTCTCCCGA 
ACTTTGCTGG AGCTCCAGTC TGCACTCGAA GGGTCCGACC CAAAATTTGT TGGTTTATTT 
GTTAAAGCTC TTGGATTTGT CGTTCGGGTC GGGTTTCAAA GGAATCATGG GTCGTGGCGT 
TTTTCTTCTA TTGAGAATCA CCCCTTTGTT ATGATTTTGT CATCAAGAAC TGAGGTTCAG 
TCTGAATTGG TTCAGCAAGT CTTACTATTT TTCGGACAGA ATAGAAGATC GGGAATGGTG 
GAAATTTGTG AATTCTTGAG ACCCTTTTTG AATTTTTGGA TTCTACGCGT GCCCTTTTCG 
AATTCTTCGT CATCTTTGTT TGCAAGGCAG TTGATATCAT CAATGGCTTC GTTTTGTTGT 
TCTTTCCCTG ATGAGGCGAT TCCGGTTTTA AAGCTACTGA TAGGTTGCCT TAAGCATGCG 
TCACATAAGA ATTCAGATGA GCTTAAGAAC TCATATTATT TTCTGGAATC CATAGTTGAT 
GCATATACGG TCGTTTTGAG ACACTTGGTT GGAACCGGAC TGCTGGTTAC TGAGGCTCAA 
CTGTTTGGTG TGGAACTGTC AGATGCTATT CTTTCATTAT TGACATGTCA TCACCGGCAT 
GCTGGTAGTA GTGAACCAAT TGTTGAACTA GTGAAGCGGC TGTTTGTTGC CCAGAAAAAT 
CTTTCTTTGC AATATATGCC TGAAATATCA TCAACACTGC TATCCTTATT TGTTGTCCTG 
ATTCAATCTG ATCTTGAATA CGAACAACTC TCTTTATTGA AACTCCTGAA CTTCCTTTTA 
AAATGGAAAA GTGAAAAGGA ATATGAGGTT GATAGAGTCA AATGTGCTAC GAGCGAAGAG 
CTTTTGTTCA CATTTCCTGT CATCAACCTA TTATCATCTA CATCTAGATC TATTAAAGGA 
GAAGCAGCAG AATTGCTTGT GACGCTGGAA AAGGTCCTAG TAGAACTGTC AAAAGCACCA 
AAGGCTGGAC TAGCCAAAGA AGGGGGATGC CCCCCTATCA GCTCTCTAGG ATCGATTGCT 
TATAGGCTTT TGCGATGCTT GTGGTTTCAG GACCAGTTTT TACTGCCCAC TTCCTTCCTG 
AATTTTGCTT CCAGTGGCAA AACGGATGTC AAAGTAATGC ACCAGAAACC GAGACATTGG 
GCTTCTCAGT TAAGAGAATA CATTTTGTCG ATTGTAGACA GAAGAAAATC CTCCCTATCT 
GTTTCTCAGT CTCAAGAACG TTTCACAAGG GAACTGCCCC CTTTACTCGG TGCCATTACT 
GGTGTTTTGG TGATGCACCG GTCATTTGGA GATACGGCAA TTGATTTGCT GGGTGCTATT 
GGCATTGTGG ACCCCAAACA GGGAGTGCCA CTGCTGCTAG CAATTCTATT TTATAGTAAC 
ATCTTCACCA GTAAAGACAT TAGTTACCAG AATATGTTGC CAAAACTTCT GGCATTACTT 
CCATCACTTG CTTCACATTC TGTGATGATT CCCCTCATAA TTCAGACAAT CTTGCCGATG 
CTTCAGAAAG ATGGAAAACC TGTGTTGTAT GCCACCGGCG CTCGTTTGCT TTGTCAGACC 
TGGGCAATCA ATGACCGTGC CTTTGGGAGT TTGCAAGCAA TATTGCTTCC GAAGGGGCTC 
ACTGAATTCA AGCATGAGAG AAACATCTTA ATAAGTTTGG CTGCCTCCAT TCGGGACATT 
TGCAGAAAGA ACCCTGATAG GGGGGTGGAT CTTATCTTAT CTGTTTCGGC TTGCATTGAG 
AGCCAAGATC ATATTATTAA AGCTCTTGGA TTTCAGAGCC TTGCACATCT ATGTGAAGCT 
GATGTAATTG ATTTTTATAC TGCCTGGGAT GTCATTGGTA AGCATGCGGT AGACTACACC 
ACAGACCCGG CTCTTGCACA AAGCATATGC CTCCTTCTGA GATGGGGTGC AATGGATGCT 
GAAGCATATT CAGAAGCTTC AAGGAATGTC TTGCAAATTT TATGGGGTAT TGGAACTGCT 
GTACATGTCA GTCATGCACT TGAGTGGGCA AGGGCAAGGA TCTTTGCCTT TGAGGCCCTG 
AGCCAATATG AGGTTACACA TATACAAATA GGAATTCCAG ATTTTAAGAC AGTGAACACG 
GATTTGCTTT TACGTGAGAC GAATCTTGAT GTGCTCACGG CTATGGAAGG ATTTCAAGTC 
AAGATCATAA CTCATGAACA TGTGAATCGT CGAAGGTTGG TGAAGGAGAA AAAAATTGCT 
GGAAGTAAGA TTGAGAAGTT GCTAAATGTA TTTCCACAGG TTCTCGTCTC AGGGATAAAA 
GGTAGTGCTG GGCAATTACC TGGGGCAGCT CTGTTATGCC TTTCCTTTAC CCCTAAAGAT 
GTGAACAGCC AATGCTTGTC AAGAGTATCT GTAGATTTCC ATGCTGGATA TGAGAGTGCA 
CTGGTGGAAA TAGCTGCTTC TCTTCAATTA TCAAGAAATA TTTTCACTGC CCTTCTTTCA 
TTGCAATCTT GGAAGTCCTT CATGCGGCGT TGGATAAGGG CAAATATTTC ATCCCTTGAT 
GCTAAAGCAC CATCTGTTTC ATTAGATAAA ACTTCTAAAG CTGCTACCGA TATCCTGAAG 
CGTGTGATGC GACTTGCTGA AGAGTCTATC CCAAGTTCTG CTGAAAATAT TGCTCTGGCT 
ATTGGTGCAC TCTGTGTGGT GTTGGCTCCA TCTACCCATA CTGTCAAATC AACTGCTTCA 
AAGTTTCTGC TAAACTGGTT GTTCCAGAAT GAGCATGATC ATCGCCAATG GTCAGCCGCA 
ATATCTCTTG GATTGGTTTC AAGTTGTCTT CATGTAACTG ATCACAAACA GAAATTTGAG 
AATATCACTG GACTTATAAA GGTTTTGCAT GGCAGCAAAA GCATCCTTGT CAAAGGAGCT 
TGTGGGCTTG GCTTGGGCTT TGCATGCCAA GACCTTCTTA CCAGGTTTGA AGCTGCTGAT 
AATGTTGATT TGGATAAAGA AAAGTACAAG GCACAGGAGG TTGACTTATT AGGGAAGATT 
CTTAGGACAC TACTTCTGAT GACAAGCCAG CTCAGTAATG CTTCATATGA TATTCTAGAA 
AGTCTTCCTC CATTTTTCAG CATGGGTGCT AATGATATGG AAATCAATTT GACATCTGAC 
CAGTTGCTTG AGAAGTGTGA TGACTTGGAG GAAGATCCTT GGGGTGTTGC TGGTCTTGTC 
CTTGGCTTGG GGATCTCTTT CAGTGCAATA TATAGAGCTG GGGCACATGA TGCTATGCTG 
AAGATAAAGG ACTTGATTAT ATCATGGATC CCATATGTAA ACTCTCTAGT AACGAACTCA 
TCTTTTTCCA GTGAAGGAAG GGAGAAAGCT TTATCAGTAG GATCCTGTCT TGCACTTCCC 
AGTGTTGTGG CATTCTGCCG GAGAGTGGAA ATGATCAATG ATAATGAGCT GGATCAACTA 
TTAAAGGGGT ATCATGAGCT CATATCTGAG TTATTATCTG TTAAAAAATC TGGTACTTAC 
CATCAGAGCT TGATGTTGGC ATCATGCATT GGTGCTGGAA GTCTTATCGC TTGCATTTTG 
AATGAAGGTG TGCACCCTCT TGAAGCTGAA TTTGTAAAAG GTTTACTGGA GATGTTTAGA 
AAATGCTACT GTAGTTCTTT CCCTCCTATC ATCCATTTGG GTGGGATGCT TGGAGTTGTT 
AATGCAATGG GAGCTGGTGC AGGGATTTTG GTTCATGCCC ATCACTTCTC AGCCTCAATC 
AAGACTGCTT GTGAACAAAA GGAATCTTCT CATATTTTGG GCCCTTTACT TTCAAGCCCT 
TTTTGTGAGC CACATTTGAC AACATTGGTG CAAGAGATAT TTCTCATTGC GCAAAATTCT 
GATGATCTTA AAATGCAACA AAATGCAGCT TGGGCCGTTT CATTCCTTCG AAATGGTTTG 
TGGTCAAAGG AACTTCTAAA TGCTGAAAGC AATGATCAGA CTGATGTTGT TGATAGCAAA 
ACAATTTCTC ACAATTTTCC TGAGGACAAT TTAGTTATGA AGCTGACTAT ATGGTTAATG 
CATTTAAATA ACTCCGGGGC AGGGGCTATT GCACATGTGG GCACTGTTGT AACAGTTTTA 
AGATGCCTTT CCCGAGCTCC TAGATTGCCA ACAGTGGATT GGGGATTGAT CATTAGACGC 
TGCATGAGAT ATGAGGCTCA AGTGTCTGAG GTGTTGTTGC CAGATTCAGC TCTTAAGAGG 
GGAGCCCTTC GGGAGGAATG TGTACAATTC TCAATAGCTC ATGCGAATCA ATTTGATCCC 
CTCCTGACTT TCCTTGACGA GCTATCTGAT TTGACTAGGT TCAGGACACT AGAGCTGAAT 
CTACAGTCAT GTCTGCTCTT TCATCTTGCA GGCCTGATAA AAGTATTCTC AGGTTCCAGG 
CTAGAGAAAC TGCTTGATGA TATTGCTGAA TACTTTTGCT CAGATATTTT ATACCAAGGG 
TATAGCTCTG ATCAGAAGAG CTCATTGAGA ATCTCATGCT GGGTGGGTCT CTATCAGTGT 
CTAGAAGAAG CTGTCCTTAG CTCTGTAGAG TACATATCAA ACCTTGAAAA GTGCATAGAG 
GTGCTGTTTC ACTTGTTGCC TGCATCAGAA TCTGCTGCCT TCACAGGAGT GGATCTGCCA 
AACGCTGCAG AGGAATGGCG TGTGGCAGTT CAATGCTTGG CAAAGGCTCA AGGGGATTGG 
TTATTGGATT TCCTGCAGGT TCCATTAGGG GATCTTGTGC AAGGAGGTAG CCAGTCAAAT 
GAAGTCCTAA AGAAGATTCT AGCGAAAGTG AAGCTAGTTA GGATGGGTTC CATCCCATTA 
ACTGAGCTTG GAAGATTAAA AGCTTATATG CTGAACTCTA AATCAAAGGA TATTTGGAAT 
TTGCATGCTG AAGTGGTAGC AGCTTTGCAA TATGCAGATG GTAGTGTCAA AAGACAGTGG 
CTTGTTGATG CAGTGGAGAT TAGTTGTGTC TCGAGCTATC CTTCCATAGC CTTAAAGTTT 
CTCGGTCTGC TATCTGGTAG CTGTTGCAAA TATGGGTCTC TTCTGACTTT GGACCAGCTA 
TCCGTGTTGA GTGATCTACC AGTTACCCTC CCTTCCCTTG TGACCGAACC TAGTTGGGAA 
GTTGTTGCGG AATCCATTGT TTCTACCCTT TGGACATCAA CCGAACGCAT ATACTACTTG 
GTTACTGACA AAGGACCCCC TGACAATACA AATAGCACGC AACCTATTGA CGGAAGTGAA 
AAGGATATTG CCTCCTTTCT ATTGCATGTG ATGTATCATA CCTGTACATG TTTAAAAGAG 
TATTTGCCTT TGGAGAAGCA GCTTAGGCTT GCCAATATGC TGGTCACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024465306.1
CDS	33..5603
Translation	MDSYTPLLQKTRVPQPSLQKFAVISIFSKLRSAPSYLDPDSEPGREAISQCLRSASPSVVDQSVRELCRLLLDSRLDLSRTLLELQSALEGSDPKFVGLFVKALGFVVRVGFQRNHGSWRFSSIENHPFVMILSSRTEVQSELVQQVLLFFGQNRRSGMVEICEFLRPFLNFWILRVPFSNSSSSLFARQLISSMASFCCSFPDEAIPVLKLLIGCLKHASHKNSDELKNSYYFLESIVDAYTVVLRHLVGTGLLVTEAQLFGVELSDAILSLLTCHHRHAGSSEPIVELVKRLFVAQKNLSLQYMPEISSTLLSLFVVLIQSDLEYEQLSLLKLLNFLLKWKSEKEYEVDRVKCATSEELLFTFPVINLLSSTSRSIKGEAAELLVTLEKVLVELSKAPKAGLAKEGGCPPISSLGSIAYRLLRCLWFQDQFLLPTSFLNFASSGKTDVKVMHQKPRHWASQLREYILSIVDRRKSSLSVSQSQERFTRELPPLLGAITGVLVMHRSFGDTAIDLLGAIGIVDPKQGVPLLLAILFYSNIFTSKDISYQNMLPKLLALLPSLASHSVMIPLIIQTILPMLQKDGKPVLYATGARLLCQTWAINDRAFGSLQAILLPKGLTEFKHERNILISLAASIRDICRKNPDRGVDLILSVSACIESQDHIIKALGFQSLAHLCEADVIDFYTAWDVIGKHAVDYTTDPALAQSICLLLRWGAMDAEAYSEASRNVLQILWGIGTAVHVSHALEWARARIFAFEALSQYEVTHIQIGIPDFKTVNTDLLLRETNLDVLTAMEGFQVKIITHEHVNRRRLVKEKKIAGSKIEKLLNVFPQVLVSGIKGSAGQLPGAALLCLSFTPKDVNSQCLSRVSVDFHAGYESALVEIAASLQLSRNIFTALLSLQSWKSFMRRWIRANISSLDAKAPSVSLDKTSKAATDILKRVMRLAEESIPSSAENIALAIGALCVVLAPSTHTVKSTASKFLLNWLFQNEHDHRQWSAAISLGLVSSCLHVTDHKQKFENITGLIKVLHGSKSILVKGACGLGLGFACQDLLTRFEAADNVDLDKEKYKAQEVDLLGKILRTLLLMTSQLSNASYDILESLPPFFSMGANDMEINLTSDQLLEKCDDLEEDPWGVAGLVLGLGISFSAIYRAGAHDAMLKIKDLIISWIPYVNSLVTNSSFSSEGREKALSVGSCLALPSVVAFCRRVEMINDNELDQLLKGYHELISELLSVKKSGTYHQSLMLASCIGAGSLIACILNEGVHPLEAEFVKGLLEMFRKCYCSSFPPIIHLGGMLGVVNAMGAGAGILVHAHHFSASIKTACEQKESSHILGPLLSSPFCEPHLTTLVQEIFLIAQNSDDLKMQQNAAWAVSFLRNGLWSKELLNAESNDQTDVVDSKTISHNFPEDNLVMKLTIWLMHLNNSGAGAIAHVGTVVTVLRCLSRAPRLPTVDWGLIIRRCMRYEAQVSEVLLPDSALKRGALREECVQFSIAHANQFDPLLTFLDELSDLTRFRTLELNLQSCLLFHLAGLIKVFSGSRLEKLLDDIAEYFCSDILYQGYSSDQKSSLRISCWVGLYQCLEEAVLSSVEYISNLEKCIEVLFHLLPASESAAFTGVDLPNAAEEWRVAVQCLAKAQGDWLLDFLQVPLGDLVQGGSQSNEVLKKILAKVKLVRMGSIPLTELGRLKAYMLNSKSKDIWNLHAEVVAALQYADGSVKRQWLVDAVEISCVSSYPSIALKFLGLLSGSCCKYGSLLTLDQLSVLSDLPVTLPSLVTEPSWEVVAESIVSTLWTSTERIYYLVTDKGPPDNTNSTQPIDGSEKDIASFLLHVMYHTCTCLKEYLPLEKQLRLANMLVT

Target ORF information:

RefSeq Version	XM_024609538.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa protein RST1 (LOC7490707), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024609538.1

ORF Insert Sequence:

ATGGACTCTT ACACTCCTTT ACTCCAGAAA ACACGGGTTC CACAACCCTC GCTCCAAAAA 
TTCGCGGTGA TTTCAATCTT CTCGAAGCTA CGATCCGCGC CGAGTTACCT CGACCCGGAT 
TCCGAACCCG GTAGAGAAGC TATCTCTCAA TGCCTCCGCT CCGCTTCTCC ATCCGTCGTC 
GACCAGTCTG TACGCGAGCT CTGTCGCCTC CTATTGGACT CCAGATTGGA CCTCTCCCGA 
ACTTTGCTGG AGCTCCAGTC TGCACTCGAA GGGTCCGACC CAAAATTTGT TGGTTTATTT 
GTTAAAGCTC TTGGATTTGT CGTTCGGGTC GGGTTTCAAA GGAATCATGG GTCGTGGCGT 
TTTTCTTCTA TTGAGAATCA CCCCTTTGTT ATGATTTTGT CATCAAGAAC TGAGGTTCAG 
TCTGAATTGG TTCAGCAAGT CTTACTATTT TTCGGACAGA ATAGAAGATC GGGAATGGTG 
GAAATTTGTG AATTCTTGAG ACCCTTTTTG AATTTTTGGA TTCTACGCGT GCCCTTTTCG 
AATTCTTCGT CATCTTTGTT TGCAAGGCAG TTGATATCAT CAATGGCTTC GTTTTGTTGT 
TCTTTCCCTG ATGAGGCGAT TCCGGTTTTA AAGCTACTGA TAGGTTGCCT TAAGCATGCG 
TCACATAAGA ATTCAGATGA GCTTAAGAAC TCATATTATT TTCTGGAATC CATAGTTGAT 
GCATATACGG TCGTTTTGAG ACACTTGGTT GGAACCGGAC TGCTGGTTAC TGAGGCTCAA 
CTGTTTGGTG TGGAACTGTC AGATGCTATT CTTTCATTAT TGACATGTCA TCACCGGCAT 
GCTGGTAGTA GTGAACCAAT TGTTGAACTA GTGAAGCGGC TGTTTGTTGC CCAGAAAAAT 
CTTTCTTTGC AATATATGCC TGAAATATCA TCAACACTGC TATCCTTATT TGTTGTCCTG 
ATTCAATCTG ATCTTGAATA CGAACAACTC TCTTTATTGA AACTCCTGAA CTTCCTTTTA 
AAATGGAAAA GTGAAAAGGA ATATGAGGTT GATAGAGTCA AATGTGCTAC GAGCGAAGAG 
CTTTTGTTCA CATTTCCTGT CATCAACCTA TTATCATCTA CATCTAGATC TATTAAAGGA 
GAAGCAGCAG AATTGCTTGT GACGCTGGAA AAGGTCCTAG TAGAACTGTC AAAAGCACCA 
AAGGCTGGAC TAGCCAAAGA AGGGGGATGC CCCCCTATCA GCTCTCTAGG ATCGATTGCT 
TATAGGCTTT TGCGATGCTT GTGGTTTCAG GACCAGTTTT TACTGCCCAC TTCCTTCCTG 
AATTTTGCTT CCAGTGGCAA AACGGATGTC AAAGTAATGC ACCAGAAACC GAGACATTGG 
GCTTCTCAGT TAAGAGAATA CATTTTGTCG ATTGTAGACA GAAGAAAATC CTCCCTATCT 
GTTTCTCAGT CTCAAGAACG TTTCACAAGG GAACTGCCCC CTTTACTCGG TGCCATTACT 
GGTGTTTTGG TGATGCACCG GTCATTTGGA GATACGGCAA TTGATTTGCT GGGTGCTATT 
GGCATTGTGG ACCCCAAACA GGGAGTGCCA CTGCTGCTAG CAATTCTATT TTATAGTAAC 
ATCTTCACCA GTAAAGACAT TAGTTACCAG AATATGTTGC CAAAACTTCT GGCATTACTT 
CCATCACTTG CTTCACATTC TGTGATGATT CCCCTCATAA TTCAGACAAT CTTGCCGATG 
CTTCAGAAAG ATGGAAAACC TGTGTTGTAT GCCACCGGCG CTCGTTTGCT TTGTCAGACC 
TGGGCAATCA ATGACCGTGC CTTTGGGAGT TTGCAAGCAA TATTGCTTCC GAAGGGGCTC 
ACTGAATTCA AGCATGAGAG AAACATCTTA ATAAGTTTGG CTGCCTCCAT TCGGGACATT 
TGCAGAAAGA ACCCTGATAG GGGGGTGGAT CTTATCTTAT CTGTTTCGGC TTGCATTGAG 
AGCCAAGATC ATATTATTAA AGCTCTTGGA TTTCAGAGCC TTGCACATCT ATGTGAAGCT 
GATGTAATTG ATTTTTATAC TGCCTGGGAT GTCATTGGTA AGCATGCGGT AGACTACACC 
ACAGACCCGG CTCTTGCACA AAGCATATGC CTCCTTCTGA GATGGGGTGC AATGGATGCT 
GAAGCATATT CAGAAGCTTC AAGGAATGTC TTGCAAATTT TATGGGGTAT TGGAACTGCT 
GTACATGTCA GTCATGCACT TGAGTGGGCA AGGGCAAGGA TCTTTGCCTT TGAGGCCCTG 
AGCCAATATG AGGTTACACA TATACAAATA GGAATTCCAG ATTTTAAGAC AGTGAACACG 
GATTTGCTTT TACGTGAGAC GAATCTTGAT GTGCTCACGG CTATGGAAGG ATTTCAAGTC 
AAGATCATAA CTCATGAACA TGTGAATCGT CGAAGGTTGG TGAAGGAGAA AAAAATTGCT 
GGAAGTAAGA TTGAGAAGTT GCTAAATGTA TTTCCACAGG TTCTCGTCTC AGGGATAAAA 
GGTAGTGCTG GGCAATTACC TGGGGCAGCT CTGTTATGCC TTTCCTTTAC CCCTAAAGAT 
GTGAACAGCC AATGCTTGTC AAGAGTATCT GTAGATTTCC ATGCTGGATA TGAGAGTGCA 
CTGGTGGAAA TAGCTGCTTC TCTTCAATTA TCAAGAAATA TTTTCACTGC CCTTCTTTCA 
TTGCAATCTT GGAAGTCCTT CATGCGGCGT TGGATAAGGG CAAATATTTC ATCCCTTGAT 
GCTAAAGCAC CATCTGTTTC ATTAGATAAA ACTTCTAAAG CTGCTACCGA TATCCTGAAG 
CGTGTGATGC GACTTGCTGA AGAGTCTATC CCAAGTTCTG CTGAAAATAT TGCTCTGGCT 
ATTGGTGCAC TCTGTGTGGT GTTGGCTCCA TCTACCCATA CTGTCAAATC AACTGCTTCA 
AAGTTTCTGC TAAACTGGTT GTTCCAGAAT GAGCATGATC ATCGCCAATG GTCAGCCGCA 
ATATCTCTTG GATTGGTTTC AAGTTGTCTT CATGTAACTG ATCACAAACA GAAATTTGAG 
AATATCACTG GACTTATAAA GGTTTTGCAT GGCAGCAAAA GCATCCTTGT CAAAGGAGCT 
TGTGGGCTTG GCTTGGGCTT TGCATGCCAA GACCTTCTTA CCAGGTTTGA AGCTGCTGAT 
AATGTTGATT TGGATAAAGA AAAGTACAAG GCACAGGAGG TTGACTTATT AGGGAAGATT 
CTTAGGACAC TACTTCTGAT GACAAGCCAG CTCAGTAATG CTTCATATGA TATTCTAGAA 
AGTCTTCCTC CATTTTTCAG CATGGGTGCT AATGATATGG AAATCAATTT GACATCTGAC 
CAGTTGCTTG AGAAGTGTGA TGACTTGGAG GAAGATCCTT GGGGTGTTGC TGGTCTTGTC 
CTTGGCTTGG GGATCTCTTT CAGTGCAATA TATAGAGCTG GGGCACATGA TGCTATGCTG 
AAGATAAAGG ACTTGATTAT ATCATGGATC CCATATGTAA ACTCTCTAGT AACGAACTCA 
TCTTTTTCCA GTGAAGGAAG GGAGAAAGCT TTATCAGTAG GATCCTGTCT TGCACTTCCC 
AGTGTTGTGG CATTCTGCCG GAGAGTGGAA ATGATCAATG ATAATGAGCT GGATCAACTA 
TTAAAGGGGT ATCATGAGCT CATATCTGAG TTATTATCTG TTAAAAAATC TGGTACTTAC 
CATCAGAGCT TGATGTTGGC ATCATGCATT GGTGCTGGAA GTCTTATCGC TTGCATTTTG 
AATGAAGGTG TGCACCCTCT TGAAGCTGAA TTTGTAAAAG GTTTACTGGA GATGTTTAGA 
AAATGCTACT GTAGTTCTTT CCCTCCTATC ATCCATTTGG GTGGGATGCT TGGAGTTGTT 
AATGCAATGG GAGCTGGTGC AGGGATTTTG GTTCATGCCC ATCACTTCTC AGCCTCAATC 
AAGACTGCTT GTGAACAAAA GGAATCTTCT CATATTTTGG GCCCTTTACT TTCAAGCCCT 
TTTTGTGAGC CACATTTGAC AACATTGGTG CAAGAGATAT TTCTCATTGC GCAAAATTCT 
GATGATCTTA AAATGCAACA AAATGCAGCT TGGGCCGTTT CATTCCTTCG AAATGGTTTG 
TGGTCAAAGG AACTTCTAAA TGCTGAAAGC AATGATCAGA CTGATGTTGT TGATAGCAAA 
ACAATTTCTC ACAATTTTCC TGAGGACAAT TTAGTTATGA AGCTGACTAT ATGGTTAATG 
CATTTAAATA ACTCCGGGGC AGGGGCTATT GCACATGTGG GCACTGTTGT AACAGTTTTA 
AGATGCCTTT CCCGAGCTCC TAGATTGCCA ACAGTGGATT GGGGATTGAT CATTAGACGC 
TGCATGAGAT ATGAGGCTCA AGTGTCTGAG GTGTTGTTGC CAGATTCAGC TCTTAAGAGG 
GGAGCCCTTC GGGAGGAATG TGTACAATTC TCAATAGCTC ATGCGAATCA ATTTGATCCC 
CTCCTGACTT TCCTTGACGA GCTATCTGAT TTGACTAGGT TCAGGACACT AGAGCTGAAT 
CTACAGTCAT GTCTGCTCTT TCATCTTGCA GGCCTGATAA AAGTATTCTC AGGTTCCAGG 
CTAGAGAAAC TGCTTGATGA TATTGCTGAA TACTTTTGCT CAGATATTTT ATACCAAGGG 
TATAGCTCTG ATCAGAAGAG CTCATTGAGA ATCTCATGCT GGGTGGGTCT CTATCAGTGT 
CTAGAAGAAG CTGTCCTTAG CTCTGTAGAG TACATATCAA ACCTTGAAAA GTGCATAGAG 
GTGCTGTTTC ACTTGTTGCC TGCATCAGAA TCTGCTGCCT TCACAGGAGT GGATCTGCCA 
AACGCTGCAG AGGAATGGCG TGTGGCAGTT CAATGCTTGG CAAAGGCTCA AGGGGATTGG 
TTATTGGATT TCCTGCAGGT TCCATTAGGG GATCTTGTGC AAGGAGGTAG CCAGTCAAAT 
GAAGTCCTAA AGAAGATTCT AGCGAAAGTG AAGCTAGTTA GGATGGGTTC CATCCCATTA 
ACTGAGCTTG GAAGATTAAA AGCTTATATG CTGAACTCTA AATCAAAGGA TATTTGGAAT 
TTGCATGCTG AAGTGGTAGC AGCTTTGCAA TATGCAGATG GTAGTGTCAA AAGACAGTGG 
CTTGTTGATG CAGTGGAGAT TAGTTGTGTC TCGAGCTATC CTTCCATAGC CTTAAAGTTT 
CTCGGTCTGC TATCTGGTAG CTGTTGCAAA TATGGGTCTC TTCTGACTTT GGACCAGCTA 
TCCGTGTTGA GTGATCTACC AGTTACCCTC CCTTCCCTTG TGACCGAACC TAGTTGGGAA 
GTTGTTGCGG AATCCATTGT TTCTACCCTT TGGACATCAA CCGAACGCAT ATACTACTTG 
GTTACTGACA AAGGACCCCC TGACAATACA AATAGCACGC AACCTATTGA CGGAAGTGAA 
AAGGATATTG CCTCCTTTCT ATTGCATGTG ATGTATCATA CCTGTACATG TTTAAAAGAG 
TATTTGCCTT TGGAGAAGCA GCTTAGGCTT GCCAATATGC TGGTCACTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POPTR_0001s35240g ( XM_024609545.1 ) cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa) -> XP_024465313.1 Populus trichocarpa protein RST1 (LOC7490707), transcript variant X2, mRNA.

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CloneID	OPa421717
Clone ID Related Accession (Same CDS sequence)	XM_024609545.1
Accession Version	XM_024609545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	protein RST1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037285.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTT ACACTCCTTT ACTCCAGAAA ACACGGGTTC CACAACCCTC GCTCCAAAAA 
TTCGCGGTGA TTTCAATCTT CTCGAAGCTA CGATCCGCGC CGAGTTACCT CGACCCGGAT 
TCCGAACCCG GTAGAGAAGC TATCTCTCAA TGCCTCCGCT CCGCTTCTCC ATCCGTCGTC 
GACCAGTCTG TACGCGAGCT CTGTCGCCTC CTATTGGACT CCAGATTGGA CCTCTCCCGA 
ACTTTGCTGG AGCTCCAGTC TGCACTCGAA GGGTCCGACC CAAAATTTGT TGGTTTATTT 
GTTAAAGCTC TTGGATTTGT CGTTCGGGTC GGGTTTCAAA GGAATCATGG GTCGTGGCGT 
TTTTCTTCTA TTGAGAATCA CCCCTTTGTT ATGATTTTGT CATCAAGAAC TGAGGTTCAG 
TCTGAATTGG TTCAGCAAGT CTTACTATTT TTCGGACAGA ATAGAAGATC GGGAATGGTG 
GAAATTTGTG AATTCTTGAG ACCCTTTTTG AATTTTTGGA TTCTACGCGT GCCCTTTTCG 
AATTCTTCGT CATCTTTGTT TGCAAGGCAG TTGATATCAT CAATGGCTTC GTTTTGTTGT 
TCTTTCCCTG ATGAGGCGAT TCCGGTTTTA AAGCTACTGA TAGGTTGCCT TAAGCATGCG 
TCACATAAGA ATTCAGATGA GCTTAAGAAC TCATATTATT TTCTGGAATC CATAGTTGAT 
GCATATACGG TCGTTTTGAG ACACTTGGTT GGAACCGGAC TGCTGGTTAC TGAGGCTCAA 
CTGTTTGGTG TGGAACTGTC AGATGCTATT CTTTCATTAT TGACATGTCA TCACCGGCAT 
GCTGGTAGTA GTGAACCAAT TGTTGAACTA GTGAAGCGGC TGTTTGTTGC CCAGAAAAAT 
CTTTCTTTGC AATATATGCC TGAAATATCA TCAACACTGC TATCCTTATT TGTTGTCCTG 
ATTCAATCTG ATCTTGAATA CGAACAACTC TCTTTATTGA AACTCCTGAA CTTCCTTTTA 
AAATGGAAAA GTGAAAAGGA ATATGAGGTT GATAGAGTCA AATGTGCTAC GAGCGAAGAG 
CTTTTGTTCA CATTTCCTGT CATCAACCTA TTATCATCTA CATCTAGATC TATTAAAGGA 
GAAGCAGCAG AATTGCTTGT GACGCTGGAA AAGGTCCTAG TAGAACTGTC AAAAGCACCA 
AAGGCTGGAC TAGCCAAAGA AGGGGGATGC CCCCCTATCA GCTCTCTAGG ATCGATTGCT 
TATAGGCTTT TGCGATGCTT GTGGTTTCAG GACCAGTTTT TACTGCCCAC TTCCTTCCTG 
AATTTTGCTT CCAGTGGCAA AACGGATGTC AAAGTAATGC ACCAGAAACC GAGACATTGG 
GCTTCTCAGT TAAGAGAATA CATTTTGTCG ATTGTAGACA GAAGAAAATC CTCCCTATCT 
GTTTCTCAGT CTCAAGAACG TTTCACAAGG GAACTGCCCC CTTTACTCGG TGCCATTACT 
GGTGTTTTGG TGATGCACCG GTCATTTGGA GATACGGCAA TTGATTTGCT GGGTGCTATT 
GGCATTGTGG ACCCCAAACA GGGAGTGCCA CTGCTGCTAG CAATTCTATT TTATAGTAAC 
ATCTTCACCA GTAAAGACAT TAGTTACCAG AATATGTTGC CAAAACTTCT GGCATTACTT 
CCATCACTTG CTTCACATTC TGTGATGATT CCCCTCATAA TTCAGACAAT CTTGCCGATG 
CTTCAGAAAG ATGGAAAACC TGTGTTGTAT GCCACCGGCG CTCGTTTGCT TTGTCAGACC 
TGGGCAATCA ATGACCGTGC CTTTGGGAGT TTGCAAGCAA TATTGCTTCC GAAGGGGCTC 
ACTGAATTCA AGCATGAGAG AAACATCTTA ATAAGTTTGG CTGCCTCCAT TCGGGACATT 
TGCAGAAAGA ACCCTGATAG GGGGGTGGAT CTTATCTTAT CTGTTTCGGC TTGCATTGAG 
AGCCAAGATC ATATTATTAA AGCTCTTGGA TTTCAGAGCC TTGCACATCT ATGTGAAGCT 
GATGTAATTG ATTTTTATAC TGCCTGGGAT GTCATTGGTA AGCATGCGGT AGACTACACC 
ACAGACCCGG CTCTTGCACA AAGCATATGC CTCCTTCTGA GATGGGGTGC AATGGATGCT 
GAAGCATATT CAGAAGCTTC AAGGAATGTC TTGCAAATTT TATGGGGTAT TGGAACTGCT 
GTACATGTCA GTCATGCACT TGAGTGGGCA AGGGCAAGGA TCTTTGCCTT TGAGGCCCTG 
AGCCAATATG AGGTTACACA TATACAAATA GGAATTCCAG ATTTTAAGAC AGTGAACACG 
GATTTGCTTT TACGTGAGAC GAATCTTGAT GTGCTCACGG CTATGGAAGG ATTTCAAGTC 
AAGATCATAA CTCATGAACA TGTGAATCGT CGAAGGTTGG TGAAGGAGAA AAAAATTGCT 
GGAAGTAAGA TTGAGAAGTT GCTAAATGTA TTTCCACAGG TTCTCGTCTC AGGGATAAAA 
GGTAGTGCTG GGCAATTACC TGGGGCAGCT CTGTTATGCC TTTCCTTTAC CCCTAAAGAT 
GTGAACAGCC AATGCTTGTC AAGAGTATCT GTAGATTTCC ATGCTGGATA TGAGAGTGCA 
CTGGTGGAAA TAGCTGCTTC TCTTCAATTA TCAAGAAATA TTTTCACTGC CCTTCTTTCA 
TTGCAATCTT GGAAGTCCTT CATGCGGCGT TGGATAAGGG CAAATATTTC ATCCCTTGAT 
GCTAAAGCAC CATCTGTTTC ATTAGATAAA ACTTCTAAAG CTGCTACCGA TATCCTGAAG 
CGTGTGATGC GACTTGCTGA AGAGTCTATC CCAAGTTCTG CTGAAAATAT TGCTCTGGCT 
ATTGGTGCAC TCTGTGTGGT GTTGGCTCCA TCTACCCATA CTGTCAAATC AACTGCTTCA 
AAGTTTCTGC TAAACTGGTT GTTCCAGAAT GAGCATGATC ATCGCCAATG GTCAGCCGCA 
ATATCTCTTG GATTGGTTTC AAGTTGTCTT CATGTAACTG ATCACAAACA GAAATTTGAG 
AATATCACTG GACTTATAAA GGTTTTGCAT GGCAGCAAAA GCATCCTTGT CAAAGGAGCT 
TGTGGGCTTG GCTTGGGCTT TGCATGCCAA GACCTTCTTA CCAGGTTTGA AGCTGCTGAT 
AATGTTGATT TGGATAAAGA AAAGTACAAG GCACAGGAGG TTGACTTATT AGGGAAGATT 
CTTAGGACAC TACTTCTGAT GACAAGCCAG CTCAGTAATG CTTCATATGA TATTCTAGAA 
AGTCTTCCTC CATTTTTCAG CATGGGTGCT AATGATATGG AAATCAATTT GACATCTGAC 
CAGTTGCTTG AGAAGTGTGA TGACTTGGAG GAAGATCCTT GGGGTGTTGC TGGTCTTGTC 
CTTGGCTTGG GGATCTCTTT CAGTGCAATA TATAGAGCTG GGGCACATGA TGCTATGCTG 
AAGATAAAGG ACTTGATTAT ATCATGGATC CCATATGTAA ACTCTCTAGT AACGAACTCA 
TCTTTTTCCA GTGAAGGAAG GGAGAAAGCT TTATCAGTAG GATCCTGTCT TGCACTTCCC 
AGTGTTGTGG CATTCTGCCG GAGAGTGGAA ATGATCAATG ATAATGAGCT GGATCAACTA 
TTAAAGGGGT ATCATGAGCT CATATCTGAG TTATTATCTG TTAAAAAATC TGGTACTTAC 
CATCAGAGCT TGATGTTGGC ATCATGCATT GGTGCTGGAA GTCTTATCGC TTGCATTTTG 
AATGAAGGTG TGCACCCTCT TGAAGCTGAA TTTGTAAAAG GTTTACTGGA GATGTTTAGA 
AAATGCTACT GTAGTTCTTT CCCTCCTATC ATCCATTTGG GTGGGATGCT TGGAGTTGTT 
AATGCAATGG GAGCTGGTGC AGGGATTTTG GTTCATGCCC ATCACTTCTC AGCCTCAATC 
AAGACTGCTT GTGAACAAAA GGAATCTTCT CATATTTTGG GCCCTTTACT TTCAAGCCCT 
TTTTGTGAGC CACATTTGAC AACATTGGTG CAAGAGATAT TTCTCATTGC GCAAAATTCT 
GATGATCTTA AAATGCAACA AAATGCAGCT TGGGCCGTTT CATTCCTTCG AAATGGTTTG 
TGGTCAAAGG AACTTCTAAA TGCTGAAAGC AATGATCAGA CTGATGTTGT TGATAGCAAA 
ACAATTTCTC ACAATTTTCC TGAGGACAAT TTAGTTATGA AGCTGACTAT ATGGTTAATG 
CATTTAAATA ACTCCGGGGC AGGGGCTATT GCACATGTGG GCACTGTTGT AACAGTTTTA 
AGATGCCTTT CCCGAGCTCC TAGATTGCCA ACAGTGGATT GGGGATTGAT CATTAGACGC 
TGCATGAGAT ATGAGGCTCA AGTGTCTGAG GTGTTGTTGC CAGATTCAGC TCTTAAGAGG 
GGAGCCCTTC GGGAGGAATG TGTACAATTC TCAATAGCTC ATGCGAATCA ATTTGATCCC 
CTCCTGACTT TCCTTGACGA GCTATCTGAT TTGACTAGGT TCAGGACACT AGAGCTGAAT 
CTACAGTCAT GTCTGCTCTT TCATCTTGCA GGCCTGATAA AAGTATTCTC AGGTTCCAGG 
CTAGAGAAAC TGCTTGATGA TATTGCTGAA TACTTTTGCT CAGATATTTT ATACCAAGGG 
TATAGCTCTG ATCAGAAGAG CTCATTGAGA ATCTCATGCT GGGTGGGTCT CTATCAGTGT 
CTAGAAGAAG CTGTCCTTAG CTCTGTAGAG TACATATCAA ACCTTGAAAA GTGCATAGAG 
GTGCTGTTTC ACTTGTTGCC TGCATCAGAA TCTGCTGCCT TCACAGGAGT GGATCTGCCA 
AACGCTGCAG AGGAATGGCG TGTGGCAGTT CAATGCTTGG CAAAGGCTCA AGGGGATTGG 
TTATTGGATT TCCTGCAGGT TCCATTAGGG GATCTTGTGC AAGGAGGTAG CCAGTCAAAT 
GAAGTCCTAA AGAAGATTCT AGCGAAAGTG AAGCTAGTTA GGATGGGTTC CATCCCATTA 
ACTGAGCTTG GAAGATTAAA AGCTTATATG CTGAACTCTA AATCAAAGGA TGGTAGTGTC 
AAAAGACAGT GGCTTGTTGA TGCAGTGGAG ATTAGTTGTG TCTCGAGCTA TCCTTCCATA 
GCCTTAAAGT TTCTCGGTCT GCTATCTGGT AGCTGTTGCA AATATGGGTC TCTTCTGACT 
TTGGACCAGC TATCCGTGTT GAGTGATCTA CCAGTTACCC TCCCTTCCCT TGTGACCGAA 
CCTAGTTGGG AAGTTGTTGC GGAATCCATT GTTTCTACCC TTTGGACATC AACCGAACGC 
ATATACTACT TGGTTACTGA CAAAGGACCC CCTGACAATA CAAATAGCAC GCAACCTATT 
GACGGAAGTG AAAAGGATAT TGCCTCCTTT CTATTGCATG TGATGTATCA TACCTGTACA 
TGTTTAAAAG AGTATTTGCC TTTGGAGAAG CAGCTTAGGC TTGCCAATAT GCTGGTCACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024465313.1
CDS	33..5555
Translation	MDSYTPLLQKTRVPQPSLQKFAVISIFSKLRSAPSYLDPDSEPGREAISQCLRSASPSVVDQSVRELCRLLLDSRLDLSRTLLELQSALEGSDPKFVGLFVKALGFVVRVGFQRNHGSWRFSSIENHPFVMILSSRTEVQSELVQQVLLFFGQNRRSGMVEICEFLRPFLNFWILRVPFSNSSSSLFARQLISSMASFCCSFPDEAIPVLKLLIGCLKHASHKNSDELKNSYYFLESIVDAYTVVLRHLVGTGLLVTEAQLFGVELSDAILSLLTCHHRHAGSSEPIVELVKRLFVAQKNLSLQYMPEISSTLLSLFVVLIQSDLEYEQLSLLKLLNFLLKWKSEKEYEVDRVKCATSEELLFTFPVINLLSSTSRSIKGEAAELLVTLEKVLVELSKAPKAGLAKEGGCPPISSLGSIAYRLLRCLWFQDQFLLPTSFLNFASSGKTDVKVMHQKPRHWASQLREYILSIVDRRKSSLSVSQSQERFTRELPPLLGAITGVLVMHRSFGDTAIDLLGAIGIVDPKQGVPLLLAILFYSNIFTSKDISYQNMLPKLLALLPSLASHSVMIPLIIQTILPMLQKDGKPVLYATGARLLCQTWAINDRAFGSLQAILLPKGLTEFKHERNILISLAASIRDICRKNPDRGVDLILSVSACIESQDHIIKALGFQSLAHLCEADVIDFYTAWDVIGKHAVDYTTDPALAQSICLLLRWGAMDAEAYSEASRNVLQILWGIGTAVHVSHALEWARARIFAFEALSQYEVTHIQIGIPDFKTVNTDLLLRETNLDVLTAMEGFQVKIITHEHVNRRRLVKEKKIAGSKIEKLLNVFPQVLVSGIKGSAGQLPGAALLCLSFTPKDVNSQCLSRVSVDFHAGYESALVEIAASLQLSRNIFTALLSLQSWKSFMRRWIRANISSLDAKAPSVSLDKTSKAATDILKRVMRLAEESIPSSAENIALAIGALCVVLAPSTHTVKSTASKFLLNWLFQNEHDHRQWSAAISLGLVSSCLHVTDHKQKFENITGLIKVLHGSKSILVKGACGLGLGFACQDLLTRFEAADNVDLDKEKYKAQEVDLLGKILRTLLLMTSQLSNASYDILESLPPFFSMGANDMEINLTSDQLLEKCDDLEEDPWGVAGLVLGLGISFSAIYRAGAHDAMLKIKDLIISWIPYVNSLVTNSSFSSEGREKALSVGSCLALPSVVAFCRRVEMINDNELDQLLKGYHELISELLSVKKSGTYHQSLMLASCIGAGSLIACILNEGVHPLEAEFVKGLLEMFRKCYCSSFPPIIHLGGMLGVVNAMGAGAGILVHAHHFSASIKTACEQKESSHILGPLLSSPFCEPHLTTLVQEIFLIAQNSDDLKMQQNAAWAVSFLRNGLWSKELLNAESNDQTDVVDSKTISHNFPEDNLVMKLTIWLMHLNNSGAGAIAHVGTVVTVLRCLSRAPRLPTVDWGLIIRRCMRYEAQVSEVLLPDSALKRGALREECVQFSIAHANQFDPLLTFLDELSDLTRFRTLELNLQSCLLFHLAGLIKVFSGSRLEKLLDDIAEYFCSDILYQGYSSDQKSSLRISCWVGLYQCLEEAVLSSVEYISNLEKCIEVLFHLLPASESAAFTGVDLPNAAEEWRVAVQCLAKAQGDWLLDFLQVPLGDLVQGGSQSNEVLKKILAKVKLVRMGSIPLTELGRLKAYMLNSKSKDGSVKRQWLVDAVEISCVSSYPSIALKFLGLLSGSCCKYGSLLTLDQLSVLSDLPVTLPSLVTEPSWEVVAESIVSTLWTSTERIYYLVTDKGPPDNTNSTQPIDGSEKDIASFLLHVMYHTCTCLKEYLPLEKQLRLANMLVT

Target ORF information:

RefSeq Version	XM_024609545.1
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa protein RST1 (LOC7490707), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024609545.1

ORF Insert Sequence:

ATGGACTCTT ACACTCCTTT ACTCCAGAAA ACACGGGTTC CACAACCCTC GCTCCAAAAA 
TTCGCGGTGA TTTCAATCTT CTCGAAGCTA CGATCCGCGC CGAGTTACCT CGACCCGGAT 
TCCGAACCCG GTAGAGAAGC TATCTCTCAA TGCCTCCGCT CCGCTTCTCC ATCCGTCGTC 
GACCAGTCTG TACGCGAGCT CTGTCGCCTC CTATTGGACT CCAGATTGGA CCTCTCCCGA 
ACTTTGCTGG AGCTCCAGTC TGCACTCGAA GGGTCCGACC CAAAATTTGT TGGTTTATTT 
GTTAAAGCTC TTGGATTTGT CGTTCGGGTC GGGTTTCAAA GGAATCATGG GTCGTGGCGT 
TTTTCTTCTA TTGAGAATCA CCCCTTTGTT ATGATTTTGT CATCAAGAAC TGAGGTTCAG 
TCTGAATTGG TTCAGCAAGT CTTACTATTT TTCGGACAGA ATAGAAGATC GGGAATGGTG 
GAAATTTGTG AATTCTTGAG ACCCTTTTTG AATTTTTGGA TTCTACGCGT GCCCTTTTCG 
AATTCTTCGT CATCTTTGTT TGCAAGGCAG TTGATATCAT CAATGGCTTC GTTTTGTTGT 
TCTTTCCCTG ATGAGGCGAT TCCGGTTTTA AAGCTACTGA TAGGTTGCCT TAAGCATGCG 
TCACATAAGA ATTCAGATGA GCTTAAGAAC TCATATTATT TTCTGGAATC CATAGTTGAT 
GCATATACGG TCGTTTTGAG ACACTTGGTT GGAACCGGAC TGCTGGTTAC TGAGGCTCAA 
CTGTTTGGTG TGGAACTGTC AGATGCTATT CTTTCATTAT TGACATGTCA TCACCGGCAT 
GCTGGTAGTA GTGAACCAAT TGTTGAACTA GTGAAGCGGC TGTTTGTTGC CCAGAAAAAT 
CTTTCTTTGC AATATATGCC TGAAATATCA TCAACACTGC TATCCTTATT TGTTGTCCTG 
ATTCAATCTG ATCTTGAATA CGAACAACTC TCTTTATTGA AACTCCTGAA CTTCCTTTTA 
AAATGGAAAA GTGAAAAGGA ATATGAGGTT GATAGAGTCA AATGTGCTAC GAGCGAAGAG 
CTTTTGTTCA CATTTCCTGT CATCAACCTA TTATCATCTA CATCTAGATC TATTAAAGGA 
GAAGCAGCAG AATTGCTTGT GACGCTGGAA AAGGTCCTAG TAGAACTGTC AAAAGCACCA 
AAGGCTGGAC TAGCCAAAGA AGGGGGATGC CCCCCTATCA GCTCTCTAGG ATCGATTGCT 
TATAGGCTTT TGCGATGCTT GTGGTTTCAG GACCAGTTTT TACTGCCCAC TTCCTTCCTG 
AATTTTGCTT CCAGTGGCAA AACGGATGTC AAAGTAATGC ACCAGAAACC GAGACATTGG 
GCTTCTCAGT TAAGAGAATA CATTTTGTCG ATTGTAGACA GAAGAAAATC CTCCCTATCT 
GTTTCTCAGT CTCAAGAACG TTTCACAAGG GAACTGCCCC CTTTACTCGG TGCCATTACT 
GGTGTTTTGG TGATGCACCG GTCATTTGGA GATACGGCAA TTGATTTGCT GGGTGCTATT 
GGCATTGTGG ACCCCAAACA GGGAGTGCCA CTGCTGCTAG CAATTCTATT TTATAGTAAC 
ATCTTCACCA GTAAAGACAT TAGTTACCAG AATATGTTGC CAAAACTTCT GGCATTACTT 
CCATCACTTG CTTCACATTC TGTGATGATT CCCCTCATAA TTCAGACAAT CTTGCCGATG 
CTTCAGAAAG ATGGAAAACC TGTGTTGTAT GCCACCGGCG CTCGTTTGCT TTGTCAGACC 
TGGGCAATCA ATGACCGTGC CTTTGGGAGT TTGCAAGCAA TATTGCTTCC GAAGGGGCTC 
ACTGAATTCA AGCATGAGAG AAACATCTTA ATAAGTTTGG CTGCCTCCAT TCGGGACATT 
TGCAGAAAGA ACCCTGATAG GGGGGTGGAT CTTATCTTAT CTGTTTCGGC TTGCATTGAG 
AGCCAAGATC ATATTATTAA AGCTCTTGGA TTTCAGAGCC TTGCACATCT ATGTGAAGCT 
GATGTAATTG ATTTTTATAC TGCCTGGGAT GTCATTGGTA AGCATGCGGT AGACTACACC 
ACAGACCCGG CTCTTGCACA AAGCATATGC CTCCTTCTGA GATGGGGTGC AATGGATGCT 
GAAGCATATT CAGAAGCTTC AAGGAATGTC TTGCAAATTT TATGGGGTAT TGGAACTGCT 
GTACATGTCA GTCATGCACT TGAGTGGGCA AGGGCAAGGA TCTTTGCCTT TGAGGCCCTG 
AGCCAATATG AGGTTACACA TATACAAATA GGAATTCCAG ATTTTAAGAC AGTGAACACG 
GATTTGCTTT TACGTGAGAC GAATCTTGAT GTGCTCACGG CTATGGAAGG ATTTCAAGTC 
AAGATCATAA CTCATGAACA TGTGAATCGT CGAAGGTTGG TGAAGGAGAA AAAAATTGCT 
GGAAGTAAGA TTGAGAAGTT GCTAAATGTA TTTCCACAGG TTCTCGTCTC AGGGATAAAA 
GGTAGTGCTG GGCAATTACC TGGGGCAGCT CTGTTATGCC TTTCCTTTAC CCCTAAAGAT 
GTGAACAGCC AATGCTTGTC AAGAGTATCT GTAGATTTCC ATGCTGGATA TGAGAGTGCA 
CTGGTGGAAA TAGCTGCTTC TCTTCAATTA TCAAGAAATA TTTTCACTGC CCTTCTTTCA 
TTGCAATCTT GGAAGTCCTT CATGCGGCGT TGGATAAGGG CAAATATTTC ATCCCTTGAT 
GCTAAAGCAC CATCTGTTTC ATTAGATAAA ACTTCTAAAG CTGCTACCGA TATCCTGAAG 
CGTGTGATGC GACTTGCTGA AGAGTCTATC CCAAGTTCTG CTGAAAATAT TGCTCTGGCT 
ATTGGTGCAC TCTGTGTGGT GTTGGCTCCA TCTACCCATA CTGTCAAATC AACTGCTTCA 
AAGTTTCTGC TAAACTGGTT GTTCCAGAAT GAGCATGATC ATCGCCAATG GTCAGCCGCA 
ATATCTCTTG GATTGGTTTC AAGTTGTCTT CATGTAACTG ATCACAAACA GAAATTTGAG 
AATATCACTG GACTTATAAA GGTTTTGCAT GGCAGCAAAA GCATCCTTGT CAAAGGAGCT 
TGTGGGCTTG GCTTGGGCTT TGCATGCCAA GACCTTCTTA CCAGGTTTGA AGCTGCTGAT 
AATGTTGATT TGGATAAAGA AAAGTACAAG GCACAGGAGG TTGACTTATT AGGGAAGATT 
CTTAGGACAC TACTTCTGAT GACAAGCCAG CTCAGTAATG CTTCATATGA TATTCTAGAA 
AGTCTTCCTC CATTTTTCAG CATGGGTGCT AATGATATGG AAATCAATTT GACATCTGAC 
CAGTTGCTTG AGAAGTGTGA TGACTTGGAG GAAGATCCTT GGGGTGTTGC TGGTCTTGTC 
CTTGGCTTGG GGATCTCTTT CAGTGCAATA TATAGAGCTG GGGCACATGA TGCTATGCTG 
AAGATAAAGG ACTTGATTAT ATCATGGATC CCATATGTAA ACTCTCTAGT AACGAACTCA 
TCTTTTTCCA GTGAAGGAAG GGAGAAAGCT TTATCAGTAG GATCCTGTCT TGCACTTCCC 
AGTGTTGTGG CATTCTGCCG GAGAGTGGAA ATGATCAATG ATAATGAGCT GGATCAACTA 
TTAAAGGGGT ATCATGAGCT CATATCTGAG TTATTATCTG TTAAAAAATC TGGTACTTAC 
CATCAGAGCT TGATGTTGGC ATCATGCATT GGTGCTGGAA GTCTTATCGC TTGCATTTTG 
AATGAAGGTG TGCACCCTCT TGAAGCTGAA TTTGTAAAAG GTTTACTGGA GATGTTTAGA 
AAATGCTACT GTAGTTCTTT CCCTCCTATC ATCCATTTGG GTGGGATGCT TGGAGTTGTT 
AATGCAATGG GAGCTGGTGC AGGGATTTTG GTTCATGCCC ATCACTTCTC AGCCTCAATC 
AAGACTGCTT GTGAACAAAA GGAATCTTCT CATATTTTGG GCCCTTTACT TTCAAGCCCT 
TTTTGTGAGC CACATTTGAC AACATTGGTG CAAGAGATAT TTCTCATTGC GCAAAATTCT 
GATGATCTTA AAATGCAACA AAATGCAGCT TGGGCCGTTT CATTCCTTCG AAATGGTTTG 
TGGTCAAAGG AACTTCTAAA TGCTGAAAGC AATGATCAGA CTGATGTTGT TGATAGCAAA 
ACAATTTCTC ACAATTTTCC TGAGGACAAT TTAGTTATGA AGCTGACTAT ATGGTTAATG 
CATTTAAATA ACTCCGGGGC AGGGGCTATT GCACATGTGG GCACTGTTGT AACAGTTTTA 
AGATGCCTTT CCCGAGCTCC TAGATTGCCA ACAGTGGATT GGGGATTGAT CATTAGACGC 
TGCATGAGAT ATGAGGCTCA AGTGTCTGAG GTGTTGTTGC CAGATTCAGC TCTTAAGAGG 
GGAGCCCTTC GGGAGGAATG TGTACAATTC TCAATAGCTC ATGCGAATCA ATTTGATCCC 
CTCCTGACTT TCCTTGACGA GCTATCTGAT TTGACTAGGT TCAGGACACT AGAGCTGAAT 
CTACAGTCAT GTCTGCTCTT TCATCTTGCA GGCCTGATAA AAGTATTCTC AGGTTCCAGG 
CTAGAGAAAC TGCTTGATGA TATTGCTGAA TACTTTTGCT CAGATATTTT ATACCAAGGG 
TATAGCTCTG ATCAGAAGAG CTCATTGAGA ATCTCATGCT GGGTGGGTCT CTATCAGTGT 
CTAGAAGAAG CTGTCCTTAG CTCTGTAGAG TACATATCAA ACCTTGAAAA GTGCATAGAG 
GTGCTGTTTC ACTTGTTGCC TGCATCAGAA TCTGCTGCCT TCACAGGAGT GGATCTGCCA 
AACGCTGCAG AGGAATGGCG TGTGGCAGTT CAATGCTTGG CAAAGGCTCA AGGGGATTGG 
TTATTGGATT TCCTGCAGGT TCCATTAGGG GATCTTGTGC AAGGAGGTAG CCAGTCAAAT 
GAAGTCCTAA AGAAGATTCT AGCGAAAGTG AAGCTAGTTA GGATGGGTTC CATCCCATTA 
ACTGAGCTTG GAAGATTAAA AGCTTATATG CTGAACTCTA AATCAAAGGA TGGTAGTGTC 
AAAAGACAGT GGCTTGTTGA TGCAGTGGAG ATTAGTTGTG TCTCGAGCTA TCCTTCCATA 
GCCTTAAAGT TTCTCGGTCT GCTATCTGGT AGCTGTTGCA AATATGGGTC TCTTCTGACT 
TTGGACCAGC TATCCGTGTT GAGTGATCTA CCAGTTACCC TCCCTTCCCT TGTGACCGAA 
CCTAGTTGGG AAGTTGTTGC GGAATCCATT GTTTCTACCC TTTGGACATC AACCGAACGC 
ATATACTACT TGGTTACTGA CAAAGGACCC CCTGACAATA CAAATAGCAC GCAACCTATT 
GACGGAAGTG AAAAGGATAT TGCCTCCTTT CTATTGCATG TGATGTATCA TACCTGTACA 
TGTTTAAAAG AGTATTTGCC TTTGGAGAAG CAGCTTAGGC TTGCCAATAT GCTGGTCACT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,D?jardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasj?rvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Lepl? JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouz? P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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POPTR_0001s35240g cDNA ORF clone, Populus trichocarpa(Populus balsamifera subsp. trichocarpa)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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