Map2k1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Map2k1
Entrez Gene ID	170851
Full Name	mitogen activated protein kinase kinase 1
Synonyms	Mek1
General protein information	Preferred Names mitogen activated protein kinase kinase 1 Names dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAP kinase kinase 1 MAP kinase/Erk kinase 1 MAPK/ERK kinase 1 MAPKK 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	kinase that activates Mapk3 (Erk1) and Mapk1 (Erk2) kinases [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017595427.1	XP_017450916.1	dual specificity mitogen-activated protein kinase kinase 1 isoform X1
NM_031643.4	NP_113831.1	dual specificity mitogen-activated protein kinase kinase 1

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	byr1	NP_593026.1
Neurospora crassa	NCU04612	XP_958855.1
Xenopus tropicalis (tropical clawed frog)	map2k1	NP_001008058.1
Homo sapiens (human)	MAP2K1	NP_002746.1
	MAP2K1	XP_001110225.1
Mus musculus (house mouse)	Map2k1	NP_032953.1
Danio rerio (zebrafish)	map2k1	NP_998584.2
Oryza sativa (rice)	Os06g0147800	NP_001056806.1
Pan troglodytes (chimpanzee)	MAP2K1	NP_001009071.1
Caenorhabditis elegans (roundworm)	mek-2	NP_491087.1
Magnaporthe oryzae (rice blast fungus)	MGG_00800	XP_003718185.1
Bos taurus (cattle)	MAP2K1	NP_001124224.1
Rattus norvegicus (Norway rat)	Map2k1	NP_113831.1
Drosophila melanogaster (fruit fly)	Dsor1	NP_511098.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001103	XP_322064.4
Arabidopsis thaliana (thale cress)	MEK1	NP_974619.1
Arabidopsis thaliana (thale cress)	MKK2	NP_194710.1
Canis lupus familiaris (dog)	MAP2K1	NP_001041559.1
Gallus gallus (chicken)	MAP2K1	NP_001005830.1

Gene Ontology
Bibliography

Related articles in PubMed

Tumor necrosis factor alpha-stimulated gene-6 (TSG-6) inhibits the inflammatory response by inhibiting the activation of P38 and JNK signaling pathway and decreases the restenosis of vein grafts in rats.
Zhang C, Zhang B, Wang H, Tao Q, Ge S, Zhai Z
Heart and vessels32(12)1536-1545(2017 Dec)

Individualized drug screening based on next generation sequencing and patient derived xenograft model for pancreatic cancer with bone metastasis.
Guan Z, Lan H, Chen X, Jiang X, Wang X, Jin K
Molecular medicine reports16(4)4784-4790(2017 Oct)

MiR-339 inhibits proliferation of pulmonary artery smooth muscle cell by targeting FGF signaling.
Chen J, Cui X, Li L, Qu J, Raj JU, Gou D
Physiological reports5(18)(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Formalin injection with and without short-term (<10 min) general isoflurane anesthesia induced the same level of phosphorylated ERK expression in spinal cord.
Title: Short-term anesthesia inhibits formalin-induced extracellular signal-regulated kinase (ERK) activation in the rostral anterior cingulate cortex but not in the spinal cord.

TGF-beta1 and FGF2 induce the epithelial-mesenchymal transition of Hertwig's epithelial root sheath through a MAPK/ERK-dependent signaling pathway.
Title: TGF-?1 and FGF2 stimulate the epithelial-mesenchymal transition of HERS cells through a MEK-dependent mechanism.

OPG regulated the proliferation of chondrocytes via MEK/ERK signaling, and directly affected chondrocytes by influencing the expression profile of ADAMTS-5 and TIMP-4.
Title: Osteoprotegerin promotes the proliferation of chondrocytes and affects the expression of ADAMTS-5 and TIMP-4 through MEK/ERK signaling.

Constitutive activation of the mitogen-activated protein kinase kinase 1 can promote ERK1/2 expression.
Title: Activation of the ERK1/2 pathway by the CaMEK gene via adeno-associated virus serotype 9 in cardiomyocytes.

nocodazole increases the ERK(Map2k1) activity to enhance Dusp1(MKP-1) expression which inhibits p38 activation induced by TNF-alpha
Title: Nocodazole increases the ERK activity to enhance MKP-1expression which inhibits p38 activation induced by TNF-?.

The following Map2k1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Map2k1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08352	NM_031643.4 Latest version!	Rattus norvegicus mitogen activated protein kinase kinase 1 (Map2k1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa62284	XM_017595427.1 Latest version!	Rattus norvegicus mitogen activated protein kinase kinase 1 (Map2k1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Map2k1 ( NM_031643.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113831.1 Rattus norvegicus mitogen activated protein kinase kinase 1 (Map2k1), mRNA.

Price & Availability↑

CloneID	ORa08352
Clone ID Related Accession (Same CDS sequence)	NM_031643.4
Accession Version	NM_031643.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Rattus norvegicus(Norway rat)
Product	dual specificity mitogen-activated protein kinase kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AI603089.1 and BC089772.1. On Mar 23, 2010 this sequence version replaced NM_031643.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC089772.1, D13341.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGATGG CTCCGCGGTT 
AACGGGACCA GCTCGGCCGA GACCAACCTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTG 
GAGCTGGACG AGCAGCAGCG GAAGCGCCTT GAGGCCTTTC TGACGCAGAA GCAGAAGGTG 
GGAGAGTTGA AGGATGATGA CTTTGAGAAG ATCAGTGAAC TGGGGGCTGG CAATGGTGGA 
GTGGTGTTCA AGGTCTCCCA CAAGCCATCT GGCCTGGTTA TGGCTAGGAA GCTAATTCAC 
CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATCCGGG AGCTGCAGGT GCTGCATGAG 
TGCAACTCCC CGTACATAGT GGGCTTCTAC GGGGCCTTCT ACAGTGACGG CGAGATCAGC 
ATCTGCATGG AGCACATGGA TGGTGGGTCC TTGGATCAAG TGCTGAAGAA AGCTGGAAGA 
ATTCCTGAGC AAATTTTAGG AAAAGTCAGC ATCGCTGTGA TAAAAGGCCT GACATATCTA 
CGAGAGAAGC ACAAGATTAT GCACAGAGAT GTCAAGCCTT CCAACATTCT AGTGAACTCA 
CGTGGGGAGA TCAAACTCTG CGATTTTGGG GTCAGCGGGC AGCTAATTGA CTCCATGGCC 
AACTCCTTCG TGGGAACAAG GTCCTACATG TCGCCTGAGA GACTCCAGGG GACTCACTAC 
TCTGTGCAGT CGGACATCTG GAGCATGGGG CTCTCTCTGG TGGAGATGGC AGTTGGAAGA 
TACCCCATTC CTCCTCCTGA TGCCAAGGAG CTGGAGCTGC TGTTTGGATG CCAGGTGGAA 
GGAGACGCGG CCGAAACGCC ACCCAGGCCA AGGACCCCTG GGAGGCCCCT CAGCTCATAT 
GGAATGGATA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT CGTCAATGAG 
CCTCCTCCAA AACTGCCCAG TGGAGTATTC AGTCTGGAAT TTCAGGATTT TGTGAATAAG 
TGCTTAATAA AGAACCCTGC AGAGAGAGCA GATCTGAAGC AGCTCATGGT ACATGCTTTC 
ATCAAGAGAT CTGATGCCGA GGAGGTAGAC TTCGCAGGCT GGCTCTGCTC CACCATTGGG 
CTTAACCAGC CCAGCACACC AACCCACGCT GCCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113831.1
CDS	48..1229
Translation	MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAASI

Target ORF information:

RefSeq Version	NM_031643.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen activated protein kinase kinase 1 (Map2k1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031643.4

ORF Insert Sequence:

ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGATGG CTCCGCGGTT 
AACGGGACCA GCTCGGCCGA GACCAACCTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTG 
GAGCTGGACG AGCAGCAGCG GAAGCGCCTT GAGGCCTTTC TGACGCAGAA GCAGAAGGTG 
GGAGAGTTGA AGGATGATGA CTTTGAGAAG ATCAGTGAAC TGGGGGCTGG CAATGGTGGA 
GTGGTGTTCA AGGTCTCCCA CAAGCCATCT GGCCTGGTTA TGGCTAGGAA GCTAATTCAC 
CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATCCGGG AGCTGCAGGT GCTGCATGAG 
TGCAACTCCC CGTACATAGT GGGCTTCTAC GGGGCCTTCT ACAGTGACGG CGAGATCAGC 
ATCTGCATGG AGCACATGGA TGGTGGGTCC TTGGATCAAG TGCTGAAGAA AGCTGGAAGA 
ATTCCTGAGC AAATTTTAGG AAAAGTCAGC ATCGCTGTGA TAAAAGGCCT GACATATCTA 
CGAGAGAAGC ACAAGATTAT GCACAGAGAT GTCAAGCCTT CCAACATTCT AGTGAACTCA 
CGTGGGGAGA TCAAACTCTG CGATTTTGGG GTCAGCGGGC AGCTAATTGA CTCCATGGCC 
AACTCCTTCG TGGGAACAAG GTCCTACATG TCGCCTGAGA GACTCCAGGG GACTCACTAC 
TCTGTGCAGT CGGACATCTG GAGCATGGGG CTCTCTCTGG TGGAGATGGC AGTTGGAAGA 
TACCCCATTC CTCCTCCTGA TGCCAAGGAG CTGGAGCTGC TGTTTGGATG CCAGGTGGAA 
GGAGACGCGG CCGAAACGCC ACCCAGGCCA AGGACCCCTG GGAGGCCCCT CAGCTCATAT 
GGAATGGATA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT CGTCAATGAG 
CCTCCTCCAA AACTGCCCAG TGGAGTATTC AGTCTGGAAT TTCAGGATTT TGTGAATAAG 
TGCTTAATAA AGAACCCTGC AGAGAGAGCA GATCTGAAGC AGCTCATGGT ACATGCTTTC 
ATCAAGAGAT CTGATGCCGA GGAGGTAGAC TTCGCAGGCT GGCTCTGCTC CACCATTGGG 
CTTAACCAGC CCAGCACACC AACCCACGCT GCCAGCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Map2k1 ( XM_017595427.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450916.1 Rattus norvegicus mitogen activated protein kinase kinase 1 (Map2k1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62284
Clone ID Related Accession (Same CDS sequence)	XM_017595427.1
Accession Version	XM_017595427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	dual specificity mitogen-activated protein kinase kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGATGG CTCCGCGGTT 
AACGGGACCA GCTCGGCCGA GACCAACCTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTG 
GAGCTGGACG AGCAGCAGCG GAAGCGCCTT GAGGCCTTTC TGACGCAGAA GCAGAAGGTG 
GGAGAGTTGA AGGATGATGA CTTTGAGAAG ATCAGTGAAC TGGGGGCTGG CAATGGTGGA 
GTGGTGTTCA AGGTCTCCCA CAAGCCATCT GGCCTGGTTA TGGCTAGGAA GCTAATTCAC 
CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATCCGGG AGCTGCAGGT GCTGCATGAG 
TGCAACTCCC CGTACATAGT GGGCTTCTAC GGGGCCTTCT ACAGTGACGG CGAGATCAGC 
ATCTGCATGG AGCACATGGA TGGTGGGTCC TTGGATCAAG TGCTGAAGAA AGCTGGAAGA 
ATTCCTGAGC AAATTTTAGG AAAAGTCAGC ATCGCTGTGA TAAAAGGCCT GACATATCTA 
CGAGAGAAGC ACAAGATTAT GCACAGAGAT GTCAAGCCTT CCAACATTCT AGTGAACTCA 
CGTGGGGAGA TCAAACTCTG CGATTTTGGG GTCAGCGGGC AGCTAATTGA CTCCATGGCC 
AACTCCTTCG TGGGAACAAG GTCCTACATG TCGCCTGAGA GACTCCAGGG GACTCACTAC 
TCTGTGCAGT CGGACATCTG GAGCATGGGG CTCTCTCTGG TGGAGATGGC AGTTGGAAGA 
TACCCCATTC CTCCTCCTGA TGCCAAGGAG CTGGAGCTGC TGTTTGGATG CCAGGTGGAA 
GGAGACGCGG CCGAAACGCC ACCCAGGCCA AGGACCCCTG GGAGGCCCCT CAGCTCATAT 
GGAATGGATA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT CGTCAATGAG 
CCAGCTGAGG AGGGTGAAAC TGTCTTTGCC CTCTTTACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450916.1
CDS	369..1370
Translation	MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPAEEGETVFALFT

Target ORF information:

RefSeq Version	XM_017595427.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitogen activated protein kinase kinase 1 (Map2k1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595427.1

ORF Insert Sequence:

ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGATGG CTCCGCGGTT 
AACGGGACCA GCTCGGCCGA GACCAACCTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTG 
GAGCTGGACG AGCAGCAGCG GAAGCGCCTT GAGGCCTTTC TGACGCAGAA GCAGAAGGTG 
GGAGAGTTGA AGGATGATGA CTTTGAGAAG ATCAGTGAAC TGGGGGCTGG CAATGGTGGA 
GTGGTGTTCA AGGTCTCCCA CAAGCCATCT GGCCTGGTTA TGGCTAGGAA GCTAATTCAC 
CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATCCGGG AGCTGCAGGT GCTGCATGAG 
TGCAACTCCC CGTACATAGT GGGCTTCTAC GGGGCCTTCT ACAGTGACGG CGAGATCAGC 
ATCTGCATGG AGCACATGGA TGGTGGGTCC TTGGATCAAG TGCTGAAGAA AGCTGGAAGA 
ATTCCTGAGC AAATTTTAGG AAAAGTCAGC ATCGCTGTGA TAAAAGGCCT GACATATCTA 
CGAGAGAAGC ACAAGATTAT GCACAGAGAT GTCAAGCCTT CCAACATTCT AGTGAACTCA 
CGTGGGGAGA TCAAACTCTG CGATTTTGGG GTCAGCGGGC AGCTAATTGA CTCCATGGCC 
AACTCCTTCG TGGGAACAAG GTCCTACATG TCGCCTGAGA GACTCCAGGG GACTCACTAC 
TCTGTGCAGT CGGACATCTG GAGCATGGGG CTCTCTCTGG TGGAGATGGC AGTTGGAAGA 
TACCCCATTC CTCCTCCTGA TGCCAAGGAG CTGGAGCTGC TGTTTGGATG CCAGGTGGAA 
GGAGACGCGG CCGAAACGCC ACCCAGGCCA AGGACCCCTG GGAGGCCCCT CAGCTCATAT 
GGAATGGATA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT CGTCAATGAG 
CCAGCTGAGG AGGGTGAAAC TGTCTTTGCC CTCTTTACAT AG