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MAP2K1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MAP2K1
Entrez Gene ID 5604
Full Name mitogen-activated protein kinase kinase 1
Synonyms CFC3, MAPKK1, MEK1, MKK1, PRKMK1
General protein information
Preferred Names
dual specificity mitogen-activated protein kinase kinase 1
Names
dual specificity mitogen-activated protein kinase kinase 1
MEK 1
MAPKK 1
MAPK/ERK kinase 1
ERK activator kinase 1
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
NP_002746.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

15

15q22.1-q22.33

Summary The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

176872

Disorder Html: Cardiofaciocutaneous syndrome, 115150 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011521783 XP_011520085 dual specificity mitogen-activated protein kinase kinase 1 isoform X1
NM_002755 NP_002746 dual specificity mitogen-activated protein kinase kinase 1



Homo sapiens (human) MAP2K1 NP_002746.1
Pan troglodytes (chimpanzee) MAP2K1 NP_001009071.1
Macaca mulatta (Rhesus monkey) MAP2K1 XP_001110225.1
Canis lupus familiaris (dog) MAP2K1 NP_001041559.1
Bos taurus (cattle) MAP2K1 NP_001124224.1
Mus musculus (house mouse) Map2k1 NP_032953.1
Rattus norvegicus (Norway rat) Map2k1 NP_113831.1
Gallus gallus (chicken) MAP2K1 NP_001005830.1
Danio rerio (zebrafish) map2k1 NP_998584.2
Drosophila melanogaster (fruit fly) Dsor1 NP_511098.1
Caenorhabditis elegans mek-2 NP_491087.1
Arabidopsis thaliana (thale cress) MEK1 NP_974619.1
Arabidopsis thaliana (thale cress) MKK2 NP_194710.1
Xenopus (Silurana) tropicalis (western clawed frog) map2k1 NP_001008058.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MAP2K1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP2K1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu59946 XM_011521783 PREDICTED: Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu27418
NM_002755 Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu59946
Clone ID Related Accession (Same CDS sequence) XM_011521783
Accession Version XM_011521783.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1116bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product dual specificity mitogen-activated protein kinase kinase 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010194.18) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAAGCTGG AGAGGACCAA CTTGGAGGCC TTGCAGAAGA AGCTGGAGGA GCTAGAGCTT 
GATGAGCAGC AGCGAAAGCG CCTTGAGGCC TTTCTTACCC AGAAGCAGAA GGTGGGAGAA
CTGAAGGATG ACGACTTTGA GAAGATCAGT GAGCTGGGGG CTGGCAATGG CGGTGTGGTG
TTCAAGGTCT CCCACAAGCC TTCTGGCCTG GTCATGGCCA GAAAGCTAAT TCATCTGGAG
ATCAAACCCG CAATCCGGAA CCAGATCATA AGGGAGCTGC AGGTTCTGCA TGAGTGCAAC
TCTCCGTACA TCGTGGGCTT CTATGGTGCG TTCTACAGCG ATGGCGAGAT CAGTATCTGC
ATGGAGCACA TGGATGGAGG TTCTCTGGAT CAAGTCCTGA AGAAAGCTGG AAGAATTCCT
GAACAAATTT TAGGAAAAGT TAGCATTGCT GTAATAAAAG GCCTGACATA TCTGAGGGAG
AAGCACAAGA TCATGCACAG AGATGTCAAG CCCTCCAACA TCCTAGTCAA CTCCCGTGGG
GAGATCAAGC TCTGTGACTT TGGGGTCAGC GGGCAGCTCA TCGACTCCAT GGCCAACTCC
TTCGTGGGCA CAAGGTCCTA CATGTCGCCA GAAAGACTCC AGGGGACTCA TTACTCTGTG
CAGTCAGACA TCTGGAGCAT GGGACTGTCT CTGGTAGAGA TGGCGGTTGG GAGGTATCCC
ATCCCTCCTC CAGATGCCAA GGAGCTGGAG CTGATGTTTG GGTGCCAGGT GGAAGGAGAT
GCGGCTGAGA CCCCACCCAG GCCAAGGACC CCCGGGAGGC CCCTTAGCTC ATACGGAATG
GACAGCCGAC CTCCCATGGC AATTTTTGAG TTGTTGGATT ACATAGTCAA CGAGCCTCCT
CCAAAACTGC CCAGTGGAGT GTTCAGTCTG GAATTTCAAG ATTTTGTGAA TAAATGCTTA
ATAAAAAACC CCGCAGAGAG AGCAGATTTG AAGCAACTCA TGGTTCATGC TTTTATCAAG
AGATCTGATG CTGAGGAAGT GGATTTTGCA GGTTGGCTCT GCTCCACCAT CGGCCTTAAC
CAGCCCAGCA CACCAACCCA TGCTGCTGGC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011520085.1
CDS67..1182
Misc Feature(1)184..1140(+)
Misc Feature(2)202..1083(+)
Misc Feature(3)220..792(+)
Misc Feature(4)220..624(+)
Misc Feature(5)229..792(+)
Misc Feature(6)229..657(+)
Misc Feature(7)241..957(+)
Misc Feature(8)619..684(+)
Misc Feature(9)661..954(+)
Translation

Target ORF information:

RefSeq Version XM_011521783
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011521783

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGAAGCTGG AGAGGACCAA CTTGGAGGCC TTGCAGAAGA AGCTGGAGGA GCTAGAGCTT 
GATGAGCAGC AGCGAAAGCG CCTTGAGGCC TTTCTTACCC AGAAGCAGAA GGTGGGAGAA
CTGAAGGATG ACGACTTTGA GAAGATCAGT GAGCTGGGGG CTGGCAATGG CGGTGTGGTG
TTCAAGGTCT CCCACAAGCC TTCTGGCCTG GTCATGGCCA GAAAGCTAAT TCATCTGGAG
ATCAAACCCG CAATCCGGAA CCAGATCATA AGGGAGCTGC AGGTTCTGCA TGAGTGCAAC
TCTCCGTACA TCGTGGGCTT CTATGGTGCG TTCTACAGCG ATGGCGAGAT CAGTATCTGC
ATGGAGCACA TGGATGGAGG TTCTCTGGAT CAAGTCCTGA AGAAAGCTGG AAGAATTCCT
GAACAAATTT TAGGAAAAGT TAGCATTGCT GTAATAAAAG GCCTGACATA TCTGAGGGAG
AAGCACAAGA TCATGCACAG AGATGTCAAG CCCTCCAACA TCCTAGTCAA CTCCCGTGGG
GAGATCAAGC TCTGTGACTT TGGGGTCAGC GGGCAGCTCA TCGACTCCAT GGCCAACTCC
TTCGTGGGCA CAAGGTCCTA CATGTCGCCA GAAAGACTCC AGGGGACTCA TTACTCTGTG
CAGTCAGACA TCTGGAGCAT GGGACTGTCT CTGGTAGAGA TGGCGGTTGG GAGGTATCCC
ATCCCTCCTC CAGATGCCAA GGAGCTGGAG CTGATGTTTG GGTGCCAGGT GGAAGGAGAT
GCGGCTGAGA CCCCACCCAG GCCAAGGACC CCCGGGAGGC CCCTTAGCTC ATACGGAATG
GACAGCCGAC CTCCCATGGC AATTTTTGAG TTGTTGGATT ACATAGTCAA CGAGCCTCCT
CCAAAACTGC CCAGTGGAGT GTTCAGTCTG GAATTTCAAG ATTTTGTGAA TAAATGCTTA
ATAAAAAACC CCGCAGAGAG AGCAGATTTG AAGCAACTCA TGGTTCATGC TTTTATCAAG
AGATCTGATG CTGAGGAAGT GGATTTTGCA GGTTGGCTCT GCTCCACCAT CGGCCTTAAC
CAGCCCAGCA CACCAACCCA TGCTGCTGGC GTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27418
Clone ID Related Accession (Same CDS sequence) NM_002755
Accession Version NM_002755.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product dual specificity mitogen-activated protein kinase kinase 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA653983.1, AK291500.1, AK312356.1, L11284.1, BG709050.1 and CA419665.1. This sequence is a reference standard in the RefSeqGene project. On Mar 12, 2008 this sequence version replaced gi:14589898. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L11284.1, AK291500.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGACGG CTCTGCAGTT 
AACGGGACCA GCTCTGCGGA GACCAACTTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTA
GAGCTTGATG AGCAGCAGCG AAAGCGCCTT GAGGCCTTTC TTACCCAGAA GCAGAAGGTG
GGAGAACTGA AGGATGACGA CTTTGAGAAG ATCAGTGAGC TGGGGGCTGG CAATGGCGGT
GTGGTGTTCA AGGTCTCCCA CAAGCCTTCT GGCCTGGTCA TGGCCAGAAA GCTAATTCAT
CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATAAGGG AGCTGCAGGT TCTGCATGAG
TGCAACTCTC CGTACATCGT GGGCTTCTAT GGTGCGTTCT ACAGCGATGG CGAGATCAGT
ATCTGCATGG AGCACATGGA TGGAGGTTCT CTGGATCAAG TCCTGAAGAA AGCTGGAAGA
ATTCCTGAAC AAATTTTAGG AAAAGTTAGC ATTGCTGTAA TAAAAGGCCT GACATATCTG
AGGGAGAAGC ACAAGATCAT GCACAGAGAT GTCAAGCCCT CCAACATCCT AGTCAACTCC
CGTGGGGAGA TCAAGCTCTG TGACTTTGGG GTCAGCGGGC AGCTCATCGA CTCCATGGCC
AACTCCTTCG TGGGCACAAG GTCCTACATG TCGCCAGAAA GACTCCAGGG GACTCATTAC
TCTGTGCAGT CAGACATCTG GAGCATGGGA CTGTCTCTGG TAGAGATGGC GGTTGGGAGG
TATCCCATCC CTCCTCCAGA TGCCAAGGAG CTGGAGCTGA TGTTTGGGTG CCAGGTGGAA
GGAGATGCGG CTGAGACCCC ACCCAGGCCA AGGACCCCCG GGAGGCCCCT TAGCTCATAC
GGAATGGACA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT AGTCAACGAG
CCTCCTCCAA AACTGCCCAG TGGAGTGTTC AGTCTGGAAT TTCAAGATTT TGTGAATAAA
TGCTTAATAA AAAACCCCGC AGAGAGAGCA GATTTGAAGC AACTCATGGT TCATGCTTTT
ATCAAGAGAT CTGATGCTGA GGAAGTGGAT TTTGCAGGTT GGCTCTGCTC CACCATCGGC
CTTAACCAGC CCAGCACACC AACCCATGCT GCTGGCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002746.1
CDS476..1657
Misc Feature(1)350..352(+)
Misc Feature(2)497..502(+)
Misc Feature(3)659..1615(+)
Misc Feature(4)677..1558(+)
Misc Feature(5)695..1267(+)
Misc Feature(6)695..1099(+)
Misc Feature(7)704..1267(+)
Misc Feature(8)704..1132(+)
Misc Feature(9)704..709(+)
Misc Feature(10)716..1432(+)
Misc Feature(11)905..913(+)
Misc Feature(12)1094..1159(+)
Misc Feature(13)1097..1111(+)
Misc Feature(14)1127..1129(+)
Misc Feature(15)1127..1129(+)
Misc Feature(16)1127..1129(+)
Misc Feature(17)1127..1129(+)
Misc Feature(18)1127..1129(+)
Misc Feature(19)1136..1429(+)
Misc Feature(20)1139..1141(+)
Misc Feature(21)1139..1141(+)
Misc Feature(22)1139..1141(+)
Misc Feature(23)1139..1141(+)
Misc Feature(24)1139..1141(+)
Misc Feature(25)1283..1396(+)
Misc Feature(26)1331..1333(+)
Misc Feature(27)1331..1333(+)
Misc Feature(28)1331..1333(+)
Misc Feature(29)1349..1351(+)
Misc Feature(30)1349..1351(+)
Misc Feature(31)1367..1369(+)
Misc Feature(32)1367..1369(+)
Misc Feature(33)1385..1387(+)
Misc Feature(34)1631..1633(+)
Exon (1)1..555
Gene:MAP2K1
Gene Synonym:
Exon (2)556..766
Gene:MAP2K1
Gene Synonym:
Exon (3)767..913
Gene:MAP2K1
Gene Synonym:
Exon (4)914..991
Gene:MAP2K1
Gene Synonym:
Exon (5)992..1043
Gene:MAP2K1
Gene Synonym:
Exon (6)1044..1168
Gene:MAP2K1
Gene Synonym:
Exon (7)1169..1370
Gene:MAP2K1
Gene Synonym:
Exon (8)1371..1435
Gene:MAP2K1
Gene Synonym:
Exon (9)1436..1497
Gene:MAP2K1
Gene Synonym:
Exon (10)1498..1543
Gene:MAP2K1
Gene Synonym:
Exon (11)1544..2586
Gene:MAP2K1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002755
Organism Homo sapiens (human)
Definition Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002755

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCCAAGA AGAAGCCGAC GCCCATCCAG CTGAACCCGG CCCCCGACGG CTCTGCAGTT 
AACGGGACCA GCTCTGCGGA GACCAACTTG GAGGCCTTGC AGAAGAAGCT GGAGGAGCTA
GAGCTTGATG AGCAGCAGCG AAAGCGCCTT GAGGCCTTTC TTACCCAGAA GCAGAAGGTG
GGAGAACTGA AGGATGACGA CTTTGAGAAG ATCAGTGAGC TGGGGGCTGG CAATGGCGGT
GTGGTGTTCA AGGTCTCCCA CAAGCCTTCT GGCCTGGTCA TGGCCAGAAA GCTAATTCAT
CTGGAGATCA AACCCGCAAT CCGGAACCAG ATCATAAGGG AGCTGCAGGT TCTGCATGAG
TGCAACTCTC CGTACATCGT GGGCTTCTAT GGTGCGTTCT ACAGCGATGG CGAGATCAGT
ATCTGCATGG AGCACATGGA TGGAGGTTCT CTGGATCAAG TCCTGAAGAA AGCTGGAAGA
ATTCCTGAAC AAATTTTAGG AAAAGTTAGC ATTGCTGTAA TAAAAGGCCT GACATATCTG
AGGGAGAAGC ACAAGATCAT GCACAGAGAT GTCAAGCCCT CCAACATCCT AGTCAACTCC
CGTGGGGAGA TCAAGCTCTG TGACTTTGGG GTCAGCGGGC AGCTCATCGA CTCCATGGCC
AACTCCTTCG TGGGCACAAG GTCCTACATG TCGCCAGAAA GACTCCAGGG GACTCATTAC
TCTGTGCAGT CAGACATCTG GAGCATGGGA CTGTCTCTGG TAGAGATGGC GGTTGGGAGG
TATCCCATCC CTCCTCCAGA TGCCAAGGAG CTGGAGCTGA TGTTTGGGTG CCAGGTGGAA
GGAGATGCGG CTGAGACCCC ACCCAGGCCA AGGACCCCCG GGAGGCCCCT TAGCTCATAC
GGAATGGACA GCCGACCTCC CATGGCAATT TTTGAGTTGT TGGATTACAT AGTCAACGAG
CCTCCTCCAA AACTGCCCAG TGGAGTGTTC AGTCTGGAAT TTCAAGATTT TGTGAATAAA
TGCTTAATAA AAAACCCCGC AGAGAGAGCA GATTTGAAGC AACTCATGGT TCATGCTTTT
ATCAAGAGAT CTGATGCTGA GGAAGTGGAT TTTGCAGGTT GGCTCTGCTC CACCATCGGC
CTTAACCAGC CCAGCACACC AACCCATGCT GCTGGCGTCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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