Htr1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Htr1a
Entrez Gene ID	24473
Full Name	5-hydroxytryptamine receptor 1A
Synonyms	5HT1A,RAT5HT1A
General protein information	Preferred Names 5-hydroxytryptamine receptor 1A Names 5-hydroxytryptamine receptor 1A 5-HT-1A 5-HT1A 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled serotonin 1A receptor serotonin 5-HT1A receptor serotonin receptor 1A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q13
Summary	regulates AMPA receptor channels in the prefrontal cortex; regulation is mediated via inhibition of Ca2+/calmodulin-dependent kinase II [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012585.1	NP_036717.1	5-hydroxytryptamine receptor 1A

Pathways from BioSystems

Homologies

Homo sapiens (human)	HTR1A	NP_000515.2
Mus musculus (house mouse)	Htr1a	NP_032334.2
Gallus gallus (chicken)	HTR1A	NP_001163999.1
Pan troglodytes (chimpanzee)	HTR1A	NP_001129094.1
Canis lupus familiaris (dog)	HTR1A	NP_001012397.1
Anopheles gambiae (African malaria mosquito)	GPR5HT1A	XP_308623.4
Xenopus tropicalis (tropical clawed frog)	htr1a	XP_004910432.1
Bos taurus (cattle)	HTR1A	XP_002696350.1
Danio rerio (zebrafish)	htr1aa	NP_001116793.1
Drosophila melanogaster (fruit fly)	5-HT1A	NP_725849.1
Caenorhabditis elegans (roundworm)	ser-4	NP_497452.1
Rattus norvegicus (Norway rat)	Htr1a	NP_036717.1
	HTR1A	XP_001083407.1

Gene Ontology
Bibliography

Related articles in PubMed

Existence of Brain 5-HT1A-5-HT2A Isoreceptor Complexes with Antagonistic Allosteric Receptor-Receptor Interactions Regulating 5-HT1A Receptor Recognition.
Borroto-Escuela DO, Li X, Tarakanov AO, Savelli D, Narv?ez M, Shumilov K, Andrade-Talavera Y, Jimenez-Beristain A, Pomierny B, D?az-Cabiale Z, Cuppini R, Ambrogini P, Lindskog M, Fuxe K
ACS omega2(8)4779-4789(2017 Aug)

TRPA1, substance P, histamine and 5-hydroxytryptamine interact in an interdependent way to induce nociception.
Fischer L, Lavoranti MI, de Oliveira Borges M, Miksza AF, Sardi NF, Martynhak BJ, Tambeli CH, Parada CA
Inflammation research : official journal of the European Histamine Research Society ... [et al.]66(4)311-322(2017 Apr)

Prenatal stress alters diazepam withdrawal syndrome and 5HT1A receptor expression in the raphe nuclei of adult rats.
Lakehayli S, Said N, El Khachibi M, El Ouahli M, Nadifi S, Hakkou F, Tazi A
Neuroscience33050-6(2016 Aug)

The novel 5-HT1A receptor agonist, NLX-112 reduces l-DOPA-induced abnormal involuntary movements in rat: A chronic administration study with microdialysis measurements.
McCreary AC, Varney MA, Newman-Tancredi A
Neuropharmacology105651-660(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study demonstrates that AITC-induced nociception in vivo depends on TRPA1 and is decreased by the blockade of the NK1 receptor for SP, the H1 receptor for histamine and the 5-HT1A and 3 receptors for 5-HT
Title: TRPA1, substance P, histamine and 5-hydroxytryptamine interact in an interdependent way to induce nociception.

Results reveal a somatosensory/viscerosensory mechanism which exerts tonic inhibitory influence on homeostatic regulation of breathing independent from the classical chemoreflex excitatory pathways.
Title: A latent serotonin-1A receptor-gated spinal afferent pathway inhibiting breathing.

Study showed that maternal exposure to chronic footshock stress increases the intensity of diazepam withdrawal symptoms and significantly reduce levels of 5HT1A receptors in the raphe nuclei of adult offspring, suggesting that prenatal stress may increase vulnerability to drugs
Title: Prenatal stress alters diazepam withdrawal syndrome and 5HT1A receptor expression in the raphe nuclei of adult rats.

Microdialysis data support the interpretation that NLX-112 reduces l-DOPA-induced abnormal involuntary movements via serotonin 5-HT1A receptor activation leading to blunting of the l-DOPA-induced elevation in striatal dopamine levels
Title: The novel 5-HT1A receptor agonist, NLX-112 reduces l-DOPA-induced abnormal involuntary movements in rat: A chronic administration study with microdialysis measurements.

inwardly rectifying K+ channels coupled to 5-HT1A autoreceptors display pharmacological properties generally expected for neuronal GIRK channels, but different from GIRK1-GIRK2 heteromers
Title: Pharmacological Characterization of 5-HT1A Autoreceptor-Coupled GIRK Channels in Rat Dorsal Raphe 5-HT Neurons.

The following Htr1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Htr1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12684	NM_012585.1 Latest version!	Rattus norvegicus 5-hydroxytryptamine receptor 1A (Htr1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Htr1a ( NM_012585.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036717.1 Rattus norvegicus 5-hydroxytryptamine receptor 1A (Htr1a), mRNA.

Price & Availability↑

CloneID	ORa12684
Clone ID Related Accession (Same CDS sequence)	NM_012585.1
Accession Version	NM_012585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	5-hydroxytryptamine receptor 1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05276.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGATGTGT TCAGTTTTGG CCAGGGCAAC AACACCACAG CGTCCCAGGA GCCCTTTGGG 
ACCGGCGGCA ACGTTACTAG CATCTCCGAC GTGACCTTCA GCTACCAAGT GATCACCTCT 
CTGTTGCTGG GTACTCTCAT TTTCTGCGCG GTGCTCGGCA ATGCCTGCGT GGTTGCTGCC 
ATCGCCCTGG AGCGCTCCCT CCAGAATGTG GCCAACTATC TCATCGGCTC CTTGGCGGTT 
ACTGATCTCA TGGTATCAGT GCTGGTGCTG CCCATGGCTG CTCTGTACCA GGTGCTCAAC 
AAGTGGACCC TGGGCCAGGT CACCTGCGAC CTGTTTATCG CCCTGGATGT ACTGTGCTGC 
ACCTCGTCCA TCCTGCACCT GTGCGCCATC GCGCTAGACA GGTACTGGGC TATCACCGAC 
CCTATAGACT ATGTGAACAA AAGGACGCCC CGGCGCGCCG CTGCGCTGAT CTCGCTCACT 
TGGCTCATTG GCTTTCTCAT CTCCATCCCG CCCATGCTGG GCTGGCGCAC CCCGGAAGAC 
CGATCGGACC CCGACGCGTG CACCATCAGC AAGGACCACG GCTACACCAT CTACTCCACT 
TTCGGCGCTT TCTATATCCC GCTGTTGCTC ATGCTGGTTC TCTACGGGCG CATCTTCAGA 
GCCGCACGCT TCCGAATCCG CAAGACTGTC AGGAAGGTGG AAAAGAAGGG AGCAGGCACC 
AGCTTAGGAA CTTCGTCGGC ACCGCCCCCC AAGAAGAGCC TGAACGGACA GCCAGGTAGT 
GGGGACTGGA GGCGCTGTGC TGAGAACAGG GCGGTGGGGA CTCCGTGCAC TAATGGGGCA 
GTGAGGCAGG GTGACGACGA GGCCACCCTG GAGGTGATCG AGGTGCACCG AGTGGGCAAC 
TCCAAAGAGC ACCTTCCTCT GCCCAGCGAG TCAGGATCTA ACTCCTATGC CCCCGCCTGC 
TTGGAGAGAA AAAATGAGCG CAATGCTGAA GCAAAGCGCA AGATGGCCTT GGCCCGTGAA 
AGGAAGACGG TGAAGACTCT GGGCATCATC ATGGGCACCT TCATCCTCTG CTGGCTGCCG 
TTTTTCATTG TGGCTCTGGT CCTGCCTTTC TGTGAAAGCA GCTGCCACAT GCCTGCGTTG 
TTGGGTGCCA TAATTAACTG GCTAGGCTAC TCCAACTCCC TGCTCAATCC GGTTATTTAT 
GCTTATTTCA ACAAAGACTT TCAAAACGCT TTTAAGAAGA TAATCAAGTG CAAGTTCTGC 
CGCCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036717.1
CDS	1..1269
Translation	MDVFSFGQGNNTTASQEPFGTGGNVTSISDVTFSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVRKVEKKGAGTSLGTSSAPPPKKSLNGQPGSGDWRRCAENRAVGTPCTNGAVRQGDDEATLEVIEVHRVGNSKEHLPLPSESGSNSYAPACLERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPALLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRR

Target ORF information:

RefSeq Version	NM_012585.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 5-hydroxytryptamine receptor 1A (Htr1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012585.1

ORF Insert Sequence:

ATGGATGTGT TCAGTTTTGG CCAGGGCAAC AACACCACAG CGTCCCAGGA GCCCTTTGGG 
ACCGGCGGCA ACGTTACTAG CATCTCCGAC GTGACCTTCA GCTACCAAGT GATCACCTCT 
CTGTTGCTGG GTACTCTCAT TTTCTGCGCG GTGCTCGGCA ATGCCTGCGT GGTTGCTGCC 
ATCGCCCTGG AGCGCTCCCT CCAGAATGTG GCCAACTATC TCATCGGCTC CTTGGCGGTT 
ACTGATCTCA TGGTATCAGT GCTGGTGCTG CCCATGGCTG CTCTGTACCA GGTGCTCAAC 
AAGTGGACCC TGGGCCAGGT CACCTGCGAC CTGTTTATCG CCCTGGATGT ACTGTGCTGC 
ACCTCGTCCA TCCTGCACCT GTGCGCCATC GCGCTAGACA GGTACTGGGC TATCACCGAC 
CCTATAGACT ATGTGAACAA AAGGACGCCC CGGCGCGCCG CTGCGCTGAT CTCGCTCACT 
TGGCTCATTG GCTTTCTCAT CTCCATCCCG CCCATGCTGG GCTGGCGCAC CCCGGAAGAC 
CGATCGGACC CCGACGCGTG CACCATCAGC AAGGACCACG GCTACACCAT CTACTCCACT 
TTCGGCGCTT TCTATATCCC GCTGTTGCTC ATGCTGGTTC TCTACGGGCG CATCTTCAGA 
GCCGCACGCT TCCGAATCCG CAAGACTGTC AGGAAGGTGG AAAAGAAGGG AGCAGGCACC 
AGCTTAGGAA CTTCGTCGGC ACCGCCCCCC AAGAAGAGCC TGAACGGACA GCCAGGTAGT 
GGGGACTGGA GGCGCTGTGC TGAGAACAGG GCGGTGGGGA CTCCGTGCAC TAATGGGGCA 
GTGAGGCAGG GTGACGACGA GGCCACCCTG GAGGTGATCG AGGTGCACCG AGTGGGCAAC 
TCCAAAGAGC ACCTTCCTCT GCCCAGCGAG TCAGGATCTA ACTCCTATGC CCCCGCCTGC 
TTGGAGAGAA AAAATGAGCG CAATGCTGAA GCAAAGCGCA AGATGGCCTT GGCCCGTGAA 
AGGAAGACGG TGAAGACTCT GGGCATCATC ATGGGCACCT TCATCCTCTG CTGGCTGCCG 
TTTTTCATTG TGGCTCTGGT CCTGCCTTTC TGTGAAAGCA GCTGCCACAT GCCTGCGTTG 
TTGGGTGCCA TAATTAACTG GCTAGGCTAC TCCAACTCCC TGCTCAATCC GGTTATTTAT 
GCTTATTTCA ACAAAGACTT TCAAAACGCT TTTAAGAAGA TAATCAAGTG CAAGTTCTGC 
CGCCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Existence of Brain 5-HT1A-5-HT2A Isoreceptor Complexes with Antagonistic Allosteric Receptor-Receptor Interactions Regulating 5-HT1A Receptor Recognition.
ACS omega2(8)4779-4789(2017 Aug)
Borroto-Escuela DO,Li X,Tarakanov AO,Savelli D,Narv?ez M,Shumilov K,Andrade-Talavera Y,Jimenez-Beristain A,Pomierny B,D?az-Cabiale Z,Cuppini R,Ambrogini P,Lindskog M,Fuxe K

TRPA1, substance P, histamine and 5-hydroxytryptamine interact in an interdependent way to induce nociception.
Inflammation research : official journal of the European Histamine Research Society ... [et al.]66(4)311-322(2017 Apr)
Fischer L,Lavoranti MI,de Oliveira Borges M,Miksza AF,Sardi NF,Martynhak BJ,Tambeli CH,Parada CA

Prenatal stress alters diazepam withdrawal syndrome and 5HT1A receptor expression in the raphe nuclei of adult rats.
Neuroscience33050-6(2016 Aug)
Lakehayli S,Said N,El Khachibi M,El Ouahli M,Nadifi S,Hakkou F,Tazi A

The novel 5-HT1A receptor agonist, NLX-112 reduces l-DOPA-induced abnormal involuntary movements in rat: A chronic administration study with microdialysis measurements.
Neuropharmacology105651-660(2016 06)
McCreary AC,Varney MA,Newman-Tancredi A