Dnase1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dnase1
Entrez Gene ID	25633
Full Name	deoxyribonuclease 1
General protein information	Preferred Names deoxyribonuclease 1 Names deoxyribonuclease-1 DNase I deoxyribonuclease I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	glycoprotein that cleaves DNA endonucleolytically to yield 5(alpha)-phosphodinucleotide and oligonucleotide end products; facilitates DNA hydrolysis in ailementary duct and other digestive tissues [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006245829.3	XP_006245891.1	deoxyribonuclease-1 isoform X1
NM_013097.2	NP_037229.1	deoxyribonuclease-1 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DNASE1	NP_776959.1
Mus musculus (house mouse)	Dnase1	NP_034191.3
Danio rerio (zebrafish)	dnase1	NP_001002674.1
Xenopus tropicalis (tropical clawed frog)	LOC100493098	XP_002932509.2
Pan troglodytes (chimpanzee)	DNASE1	XP_510773.3
Canis lupus familiaris (dog)	DNASE1	NP_001002946.1
Rattus norvegicus (Norway rat)	Dnase1	NP_037229.1
Homo sapiens (human)	DNASE1	NP_005214.2
	DNASE1	XP_001095110.1
Gallus gallus (chicken)	DNASE1	NP_996840.1

Gene Ontology
Bibliography

Related articles in PubMed

Host DNases prevent vascular occlusion by neutrophil extracellular traps.
Jim?nez-Alc?zar M, Rangaswamy C, Panda R, Bitterling J, Simsek YJ, Long AT, Bilyy R, Krenn V, Renn? C, Renn? T, Kluge S, Panzer U, Mizuta R, Mannherz HG, Kitamura D, Herrmann M, Napirei M, Fuchs TA
Science (New York, N.Y.)358(6367)1202-1206(2017 12)

Comparative characterization of rat deoxyribonuclease 1 (Dnase1) and murine deoxyribonuclease 1-like 3 (Dnase1l3).
Napirei M, Wulf S, Eulitz D, Mannherz HG, Kloeckl T
The Biochemical journal389(Pt 2)355-64(2005 Jul)

Expression pattern of the deoxyribonuclease 1 gene: lessons from the Dnase1 knockout mouse.
Napirei M, Ricken A, Eulitz D, Knoop H, Mannherz HG
The Biochemical journal380(Pt 3)929-37(2004 Jun)

DNase I primary transcript is alternatively spliced in both normal and apoptotic cells: no evidence of up-regulation in apoptosis.
Liu QY, Ribecco M, Hou Y, Walker PR, Sikorska M
DNA and cell biology16(8)911-8(1997 Aug)

Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death).
Peitsch MC, Polzar B, Stephan H, Crompton T, MacDonald HR, Mannherz HG, Tschopp J
The EMBO journal12(1)371-7(1993 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A comparison of the expression and transport of DNAse1 from rats and DNAse1L3 from mice is reported.
Title: Comparative characterization of rat deoxyribonuclease 1 (Dnase1) and murine deoxyribonuclease 1-like 3 (Dnase1l3).

results show that the chief cells of the stomach produce DNase I
Title: DNase I is present in the chief cells of human and rat stomachs.

The following Dnase1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dnase1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01974	NM_013097.2 Latest version!	Rattus norvegicus deoxyribonuclease 1 (Dnase1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa01974	XM_006245829.3 Latest version!	Rattus norvegicus deoxyribonuclease I (Dnase1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dnase1 ( NM_013097.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037229.1 Rattus norvegicus deoxyribonuclease 1 (Dnase1), mRNA.

Price & Availability↑

CloneID	ORa01974
Clone ID Related Accession (Same CDS sequence)	XM_006245829.3 , NM_013097.2
Accession Version	NM_013097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	deoxyribonuclease-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF397150.1. On Jun 9, 2003 this sequence version replaced NM_013097.1. ##Evidence-Data-START## Transcript exon combination :: BC089764.1, AF397150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMN02642745 [ECO:0000348] ##Evidence-Data-END##

ATGAGGTACA CAGGGCTGAT GGGAATACTG CTCACCCTGG TCAACCTGCT GCAGCTGGCT 
GCGACTCTGA GAATTGCAGC CTTCAACATC CGGACTTTTG GGGATACTAA GATGTCTAAT 
GCCACCCTCT CTAGCTACAT TGTGAAAATC CTGAGTCGCT ATGACATTGC TGTGGTCCAA 
GAGGTCAGAG ACACTCACCT GGTTGCTGTT GGGAAGCTAC TGGATGAACT CAATCGGGAC 
ATCCCTGACA ACTATCGCTA TATAATCAGT GAGCCGCTGG GCCGCAAAAG CTACAAGGAA 
CAGTACCTTT TTGTGTACAG GCCCAGCCAG GTGTCTGTTT TGGATAGCTA TCATTATGAC 
GATGGCTGTG AACCCTGTGG AAATGACACC TTCAGCCGAG AGCCAGCCAT TGTCAAGTTC 
TTTTCCCCAT ACACTGAGGT CCGAGAGTTT GCGATTGTGC CCTTGCACTC AGCCCCAACA 
GAGGCTGTGA GTGAGATCGA TGCCCTCTAC GATGTTTATC TAGATGTCCG GCAAAAGTGG 
GGCCTGGAGG ACATCATGTT CATGGGAGAT TTCAATGCTG GCTGCAGCTA CGTCACTTCC 
TCCCAATGGT CTTCCATTCG CCTTCGGACA AGCCCCATCT TCCAGTGGCT GATCCCTGAC 
AGTGCGGACA CCACAGCCAC ATCCACACAC TGTGCTTATG ACAGGATTGT GGTTGCTGGA 
GCTCTGCTCC AGGCTGCTGT TGTTCCCAGC TCGGCTGTCC CCTTTGACTT CCAAGCAGAA 
TACAGACTTA CCAACCAGAT GGCTGAAGCC ATCAGTGACC ATTACCCAGT GGAGGTGACA 
CTCAGAAAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037229.1
CDS	279..1133
Translation	MRYTGLMGILLTLVNLLQLAATLRIAAFNIRTFGDTKMSNATLSSYIVKILSRYDIAVVQEVRDTHLVAVGKLLDELNRDIPDNYRYIISEPLGRKSYKEQYLFVYRPSQVSVLDSYHYDDGCEPCGNDTFSREPAIVKFFSPYTEVREFAIVPLHSAPTEAVSEIDALYDVYLDVRQKWGLEDIMFMGDFNAGCSYVTSSQWSSIRLRTSPIFQWLIPDSADTTATSTHCAYDRIVVAGALLQAAVVPSSAVPFDFQAEYRLTNQMAEAISDHYPVEVTLRKT

Target ORF information:

RefSeq Version	NM_013097.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus deoxyribonuclease 1 (Dnase1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013097.2

ORF Insert Sequence:

ATGAGGTACA CAGGGCTGAT GGGAATACTG CTCACCCTGG TCAACCTGCT GCAGCTGGCT 
GCGACTCTGA GAATTGCAGC CTTCAACATC CGGACTTTTG GGGATACTAA GATGTCTAAT 
GCCACCCTCT CTAGCTACAT TGTGAAAATC CTGAGTCGCT ATGACATTGC TGTGGTCCAA 
GAGGTCAGAG ACACTCACCT GGTTGCTGTT GGGAAGCTAC TGGATGAACT CAATCGGGAC 
ATCCCTGACA ACTATCGCTA TATAATCAGT GAGCCGCTGG GCCGCAAAAG CTACAAGGAA 
CAGTACCTTT TTGTGTACAG GCCCAGCCAG GTGTCTGTTT TGGATAGCTA TCATTATGAC 
GATGGCTGTG AACCCTGTGG AAATGACACC TTCAGCCGAG AGCCAGCCAT TGTCAAGTTC 
TTTTCCCCAT ACACTGAGGT CCGAGAGTTT GCGATTGTGC CCTTGCACTC AGCCCCAACA 
GAGGCTGTGA GTGAGATCGA TGCCCTCTAC GATGTTTATC TAGATGTCCG GCAAAAGTGG 
GGCCTGGAGG ACATCATGTT CATGGGAGAT TTCAATGCTG GCTGCAGCTA CGTCACTTCC 
TCCCAATGGT CTTCCATTCG CCTTCGGACA AGCCCCATCT TCCAGTGGCT GATCCCTGAC 
AGTGCGGACA CCACAGCCAC ATCCACACAC TGTGCTTATG ACAGGATTGT GGTTGCTGGA 
GCTCTGCTCC AGGCTGCTGT TGTTCCCAGC TCGGCTGTCC CCTTTGACTT CCAAGCAGAA 
TACAGACTTA CCAACCAGAT GGCTGAAGCC ATCAGTGACC ATTACCCAGT GGAGGTGACA 
CTCAGAAAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dnase1 ( XM_006245829.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245891.1 Rattus norvegicus deoxyribonuclease I (Dnase1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01974
Clone ID Related Accession (Same CDS sequence)	XM_006245829.3 , NM_013097.2
Accession Version	XM_006245829.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deoxyribonuclease-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245829.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTACA CAGGGCTGAT GGGAATACTG CTCACCCTGG TCAACCTGCT GCAGCTGGCT 
GCGACTCTGA GAATTGCAGC CTTCAACATC CGGACTTTTG GGGATACTAA GATGTCTAAT 
GCCACCCTCT CTAGCTACAT TGTGAAAATC CTGAGTCGCT ATGACATTGC TGTGGTCCAA 
GAGGTCAGAG ACACTCACCT GGTTGCTGTT GGGAAGCTAC TGGATGAACT CAATCGGGAC 
ATCCCTGACA ACTATCGCTA TATAATCAGT GAGCCGCTGG GCCGCAAAAG CTACAAGGAA 
CAGTACCTTT TTGTGTACAG GCCCAGCCAG GTGTCTGTTT TGGATAGCTA TCATTATGAC 
GATGGCTGTG AACCCTGTGG AAATGACACC TTCAGCCGAG AGCCAGCCAT TGTCAAGTTC 
TTTTCCCCAT ACACTGAGGT CCGAGAGTTT GCGATTGTGC CCTTGCACTC AGCCCCAACA 
GAGGCTGTGA GTGAGATCGA TGCCCTCTAC GATGTTTATC TAGATGTCCG GCAAAAGTGG 
GGCCTGGAGG ACATCATGTT CATGGGAGAT TTCAATGCTG GCTGCAGCTA CGTCACTTCC 
TCCCAATGGT CTTCCATTCG CCTTCGGACA AGCCCCATCT TCCAGTGGCT GATCCCTGAC 
AGTGCGGACA CCACAGCCAC ATCCACACAC TGTGCTTATG ACAGGATTGT GGTTGCTGGA 
GCTCTGCTCC AGGCTGCTGT TGTTCCCAGC TCGGCTGTCC CCTTTGACTT CCAAGCAGAA 
TACAGACTTA CCAACCAGAT GGCTGAAGCC ATCAGTGACC ATTACCCAGT GGAGGTGACA 
CTCAGAAAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245891.1
CDS	199..1053
Translation	MRYTGLMGILLTLVNLLQLAATLRIAAFNIRTFGDTKMSNATLSSYIVKILSRYDIAVVQEVRDTHLVAVGKLLDELNRDIPDNYRYIISEPLGRKSYKEQYLFVYRPSQVSVLDSYHYDDGCEPCGNDTFSREPAIVKFFSPYTEVREFAIVPLHSAPTEAVSEIDALYDVYLDVRQKWGLEDIMFMGDFNAGCSYVTSSQWSSIRLRTSPIFQWLIPDSADTTATSTHCAYDRIVVAGALLQAAVVPSSAVPFDFQAEYRLTNQMAEAISDHYPVEVTLRKT

Target ORF information:

RefSeq Version	XM_006245829.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus deoxyribonuclease I (Dnase1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245829.3

ORF Insert Sequence:

ATGAGGTACA CAGGGCTGAT GGGAATACTG CTCACCCTGG TCAACCTGCT GCAGCTGGCT 
GCGACTCTGA GAATTGCAGC CTTCAACATC CGGACTTTTG GGGATACTAA GATGTCTAAT 
GCCACCCTCT CTAGCTACAT TGTGAAAATC CTGAGTCGCT ATGACATTGC TGTGGTCCAA 
GAGGTCAGAG ACACTCACCT GGTTGCTGTT GGGAAGCTAC TGGATGAACT CAATCGGGAC 
ATCCCTGACA ACTATCGCTA TATAATCAGT GAGCCGCTGG GCCGCAAAAG CTACAAGGAA 
CAGTACCTTT TTGTGTACAG GCCCAGCCAG GTGTCTGTTT TGGATAGCTA TCATTATGAC 
GATGGCTGTG AACCCTGTGG AAATGACACC TTCAGCCGAG AGCCAGCCAT TGTCAAGTTC 
TTTTCCCCAT ACACTGAGGT CCGAGAGTTT GCGATTGTGC CCTTGCACTC AGCCCCAACA 
GAGGCTGTGA GTGAGATCGA TGCCCTCTAC GATGTTTATC TAGATGTCCG GCAAAAGTGG 
GGCCTGGAGG ACATCATGTT CATGGGAGAT TTCAATGCTG GCTGCAGCTA CGTCACTTCC 
TCCCAATGGT CTTCCATTCG CCTTCGGACA AGCCCCATCT TCCAGTGGCT GATCCCTGAC 
AGTGCGGACA CCACAGCCAC ATCCACACAC TGTGCTTATG ACAGGATTGT GGTTGCTGGA 
GCTCTGCTCC AGGCTGCTGT TGTTCCCAGC TCGGCTGTCC CCTTTGACTT CCAAGCAGAA 
TACAGACTTA CCAACCAGAT GGCTGAAGCC ATCAGTGACC ATTACCCAGT GGAGGTGACA 
CTCAGAAAGA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Host DNases prevent vascular occlusion by neutrophil extracellular traps.
Science (New York, N.Y.)358(6367)1202-1206(2017 12)
Jim?nez-Alc?zar M,Rangaswamy C,Panda R,Bitterling J,Simsek YJ,Long AT,Bilyy R,Krenn V,Renn? C,Renn? T,Kluge S,Panzer U,Mizuta R,Mannherz HG,Kitamura D,Herrmann M,Napirei M,Fuchs TA

Comparative characterization of rat deoxyribonuclease 1 (Dnase1) and murine deoxyribonuclease 1-like 3 (Dnase1l3).
The Biochemical journal389(Pt 2)355-64(2005 Jul)
Napirei M,Wulf S,Eulitz D,Mannherz HG,Kloeckl T

Expression pattern of the deoxyribonuclease 1 gene: lessons from the Dnase1 knockout mouse.
The Biochemical journal380(Pt 3)929-37(2004 Jun)
Napirei M,Ricken A,Eulitz D,Knoop H,Mannherz HG

DNase I primary transcript is alternatively spliced in both normal and apoptotic cells: no evidence of up-regulation in apoptosis.
DNA and cell biology16(8)911-8(1997 Aug)
Liu QY,Ribecco M,Hou Y,Walker PR,Sikorska M

Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death).
The EMBO journal12(1)371-7(1993 Jan)
Peitsch MC,Polzar B,Stephan H,Crompton T,MacDonald HR,Mannherz HG,Tschopp J