Pde3b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde3b
Entrez Gene ID	29516
Full Name	phosphodiesterase 3B
General protein information	Preferred Names phosphodiesterase 3B Names cGMP-inhibited 3',5'-cyclic phosphodiesterase B CGI-PDE B CGIPDE1 cyclic GMP-inhibited phosphodiesterase B phosphodiesterase 3B, cGMP-inhibited
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q34
Summary	catalyzes the hydrolysis of cAMP and cGMP; regulates insulin secretory processes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017229.1	NP_058925.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE3B	NP_000913.2
Pan troglodytes (chimpanzee)	PDE3B	XP_003312976.1
	PDE3B	XP_001092891.1
Canis lupus familiaris (dog)	PDE3B	XP_854529.2
Bos taurus (cattle)	PDE3B	XP_002693091.1
Mus musculus (house mouse)	Pde3b	NP_035185.2
Rattus norvegicus (Norway rat)	Pde3b	NP_058925.1
Gallus gallus (chicken)	PDE3B	NP_001026353.1
Danio rerio (zebrafish)	pde3b	XP_691883.4

Gene Ontology
Bibliography

Related articles in PubMed

Proinsulin C-peptide inhibits lipolysis in diabetic rat adipose tissue through phosphodiestrase-3B enzyme.
Ghorbani A, Omrani GR, Hadjzadeh MA, Varedi M
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(3)221-5(2013 Mar)

Phosphatidylinositol 3-kinase is an upstream regulator of the phosphodiesterase 3B pathway of leptin signalling that may not involve activation of Akt in the rat hypothalamus.
Sahu A, Koshinaka K, Sahu M
Journal of neuroendocrinology25(2)168-79(2013 Feb)

Acylated and unacylated ghrelin attenuate isoproterenol-induced lipolysis in isolated rat visceral adipocytes through activation of phosphoinositide 3-kinase ? and phosphodiesterase 3B.
Baragli A, Gh? C, Arnoletti E, Granata R, Ghigo E, Muccioli G
Biochimica et biophysica acta1811(6)386-96(2011 Jun)

A phosphodiesterase 3B-based signaling complex integrates exchange protein activated by cAMP 1 and phosphatidylinositol 3-kinase signals in human arterial endothelial cells.
Wilson LS, Baillie GS, Pritchard LM, Umana B, Terrin A, Zaccolo M, Houslay MD, Maurice DH
The Journal of biological chemistry286(18)16285-96(2011 May)

Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets.
Heimann E, Jones HA, Resj? S, Manganiello VC, Stenson L, Degerman E
PloS one5(12)e14191(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C-peptide has the potential to act, conditionally, as an antilipolytic hormone by activating phosphodiesterase-3B.
Title: Proinsulin C-peptide inhibits lipolysis in diabetic rat adipose tissue through phosphodiestrase-3B enzyme.

PI3K but not Akt is an upstream regulator of the PDE3B pathway of leptin signalling in the rat hypothalamus.
Title: Phosphatidylinositol 3-kinase is an upstream regulator of the phosphodiesterase 3B pathway of leptin signalling that may not involve activation of Akt in the rat hypothalamus.

Data demonstrate that AG/UAG attenuation of ISO-induced lipolysis involves PI3Kgamma/AKT and PDE3B.
Title: Acylated and unacylated ghrelin attenuate isoproterenol-induced lipolysis in isolated rat visceral adipocytes through activation of phosphoinositide 3-kinase ? and phosphodiesterase 3B.

PDE3B is activated in response to agents relevant for beta-cell function and activation is linked to increased as well as decreased phosphorylation of the enzyme
Title: Expression and regulation of cyclic nucleotide phosphodiesterases in human and rat pancreatic islets.

PDE3 inhibition reverses leptin-induced stimulation of proopiomelanocortin and neurotensin gene expression; the PDE3B pathway plays an important role in mediating leptin action in the hypothalamus
Title: A role of phosphodiesterase-3B pathway in mediating leptin action on proopiomelanocortin and neurotensin neurons in the hypothalamus.

The following Pde3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03320	NM_017229.1 Latest version!	Rattus norvegicus phosphodiesterase 3B (Pde3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde3b ( NM_017229.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058925.1 Rattus norvegicus phosphodiesterase 3B (Pde3b), mRNA.

Price & Availability↑

CloneID	ORa03320
Clone ID Related Accession (Same CDS sequence)	NM_017229.1
Accession Version	NM_017229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Z22867.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z22867.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGGAAGG ACGAGCGCGA GCGGGACACG CCAGCCATGA GGTCCCCGCC GCCGCCGCCG 
CCGCCGGCCA CGGCCACGGC CGCCTCGCCC CCCGAGAGCC TGCGCAACGG CTACGTGAAG 
AGCTGCGTGA GCCCGCTGCG GCAGGACCCT CCGCGCAGCT TCTTCTTCCA CCTCTGCCGC 
TTCTGCAACG TGGAGCCGCC GGCGGCCTCG CTCCGCGCCG GGGCACGCCT CTCGCTCGCC 
GCTCTGGCCG CCTTTGTCCT GGCCGCGCTG CTGGGCGCGG GGCCCGAGCG CTGGGCGGCC 
GCGGCGACCG GGCTTCGGAC GCTGCTGAGC GCTTGCTCGC TCAGCCTCAG CCCGCTCTTC 
AGCATCGCCT GTGCCTTCTT CTTCCTCACC TGCTTCCTGA CGCGCGCGCA GCGCGGCCCG 
GACCGCGGCG CCGGCTCCTG GTGGCTGCTG GCGCTGCCTG CCTGCTGCTA CCTGGGCGAC 
TTCGCGGCGT GGCAGTGGTG GTCGTGGCTG CGCGGGGAGC CGGCGGCGGC GGCGGCCGGC 
CGGCTCTGCC TGGTGCTGAG CTGCGTGGGG CTGCTGACGC TCGCGCCTCG CGTGAGGCTG 
CGGCACGGAG TCCTGGTGCT GCTCTTCGCC GGCCTGGTGT GGTGGGTGTC CTTCTCCGGC 
CTCGGGGCTC TGCCGCCCGC GCTCAGGCCT CTGCTGTCGT GCCTGGTCGG GGGCGCGGGA 
TGCCTGCTAG CCCTGGGCTT GGACCACTTC TTTCACGTCC GGGGAGCCTC ACCTCCCCCG 
CGCTCGGCGA GTACCGCGGA TGAAAAAGTG CCTGTGATCA GACCCCGGAG GAGGTCCAGC 
TGCGTGTCGC TGGGAGAAAG CGCGGCCGGT TACTATGGCA GTGGCAAGAT GTTCAGGAGA 
CCGTCGTTGC CTTGTATTTC TCGAGAACAG ATGATTCTCT GGGACTGGGA CTTGAAGCAG 
TGGTGTAAAC CTCATTACCA GAATTCTGGA GGTGGAAATG GAGTTGATCT TTCAGTGCTA 
AATGAAGCTC GCAATATGGT GTCAGACCTT CTGATTGACC CAAGCCTTCC CCCACAAGTC 
ATTTCTTCTT TGCGGAGTAT CAGTAGCTTG ATGGGTGCTT TCTCAGGTTC CTGTAGGCCA 
AAGATTAATT CTTTCACACC ATTTCCTGGA TTTTATCCCT GTTCTGAAGT AGAAGATCCA 
GTAGAGAAAG GCGATCGAAA ACTTCACAAG GGGTTGAGTA GCAAACCCAG TTTCCCGACT 
GCACAGCTGA GGAGGAGCTC AGGAGCGTCA GGATTGCTGA CCAGTGAGCA CCACTCACGG 
TGGGATCGCA GCGGTGGCAA AAGGCCTTAC CAAGAACTCA GTGTTTCAAG TCATGGATGC 
CACCTAAATG GGCCTTTTAG TTCAAATCTT ATGACAATTC CAAAGCAGAG GTCATCATCT 
GTGTCGCTGA CGCACCATGC AGGTCTGAGA AGAGCTGGTG CTTTGCCCAG CCCCAGTCTT 
CTGAATTCTT CAAGCCATGT ACCCGTGTCT GCTGGCTGTC TAACTAATCG ATCACCCGTA 
GGATTTCTTG ATACCAGTGA TTTTCTTACT AAGCCAAGTG TTACTTTACA TAGATCACTG 
GGCAGTGTAT CCAGTGCAGC AGATTTCCAT CAGTACCTTA GGAACTCTGA CAGCAGTCTG 
TGTAGCAGCT GTGGACACCA AATACTCAAG TATGTTTCGA CATGTGAACC TGATGGTACA 
GACCACCACA ATGAAAAATC AGGTGAAGAA GACAGCACTG TTTTCTCAAA AGAACGATTG 
AACATTGTGG AAACTCAAGA AGAAGAGACA GTGAAGGAAG ACTGCAGGGA ATTATTTTTG 
GAAGGTGATG ATCACCTAAT GGAAGAGGCC CAGCAACCGA ATATTGACCA GGAAGTGTTA 
TTGGACCCGA TGTTAGTGGA AGATTATGAT TCATTAATAG AAAAGATGAG CAACTGGAAC 
TTTCAGATTT TTGAGCTTGT AGAAAAAATG GGAGAAAAAT CAGGAAGGAT TCTCAGTCAG 
GTTATGTATA CTTTATTTCA AGATACTGGT TTATTGGAAA CATTTAAAAT TCCCACTCAA 
GAATTTATGA ATTATTTTCG TGCACTAGAA AATGGCTACC GAGATATTCC ATATCACAAT 
CGTGTGCATG CCACAGATGT CCTACATGCT GTTTGGTATT TGACAACACG ACCAATTCCT 
GGCTTACAGC AGCTCCATAA TAACCATGAA ACAGAAACCA AAGCAGATTC AGATGCTAGA 
CTTAGTTCTG GACAGATTGC TTACCTTTCT TCGAAGAGTT GCTGTATTCC AGATAAGAGT 
TATGGCTGCC TGTCTTCAAA CATTCCTGCG TTAGAACTGA TGGCTTTATA TGTGGCAGCT 
GCCATGCACG ATTATGATCA CCCAGGAAGA ACAAATGCAT TCCTAGTGGC TACAAATGCA 
CCTCAGGCAG TTTTATACAA TGACAGATCT GTTCTAGAAA ATCATCATGC CGCATCAGCG 
TGGAATCTGT ATCTTTCTCG CCCAGAATAC AACTTCCTCC TTAACCTTGA TCACATGGAA 
TTCAAGCGTT TTCGATTTTT AGTCATAGAA GCAATCCTTG CTACAGATCT CAAAAAACAT 
TTTGATTTCC TTGCTGAATT CAATGCCAAG GCCAATGATG TAAATAGTAA CGGTATAGAA 
TGGAGCAGTG AAAACGATCG ACTCTTAGTC TGCCAGGTGT GCATCAAATT AGCAGACATC 
AACGGCCCAG CAAAAGATCG GGATCTTCAT TTGAGATGGA CAGAAGGCAT TGTGAATGAG 
TTTTATGAGC AGGGAGATGA AGAAGCAACC CTGGGTCTAC CTATTAGTCC CTTCATGGAT 
CGTTCTTCTC CTCAACTAGC AAAACTCCAA GAGTCATTTA TCACCCACAT TGTGGGCCCC 
CTGTGCAACT CCTATGATGC TGCTGGCTTG CTGCCTGGTC AGTGGATAGA AGCAGAAGAG 
GGTGACGATA CAGAAAGTGA TGATGATGAT GATGATGATG ATGATGATGA TGATGATGAT 
GATGAAGAGT TAGATTCAGA TGATGAAGAA ACAGAAGACA ATCTAAATCC TAAACCACAA 
AGAAGGAAAG GCAGGCGGCG AATATTTTGC CAACTAATGC ACCACCTCAC TGAAAACCAC 
AAGATCTGGA AGGAAATCAT AGAAGAGGAA GAAAAATGTA AAGCTGAGGG GAACAAACTG 
CAGGTGGATA ATGCCTCCTT ACCTCAGGCA GATGAGATTC AGGTCATTGA AGAAGCAGAT 
GAAGAGGAAG AACAAATGTT TGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058925.1
CDS	65..3391
Translation	MRKDERERDTPAMRSPPPPPPPATATAASPPESLRNGYVKSCVSPLRQDPPRSFFFHLCRFCNVEPPAASLRAGARLSLAALAAFVLAALLGAGPERWAAAATGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQRGPDRGAGSWWLLALPACCYLGDFAAWQWWSWLRGEPAAAAAGRLCLVLSCVGLLTLAPRVRLRHGVLVLLFAGLVWWVSFSGLGALPPALRPLLSCLVGGAGCLLALGLDHFFHVRGASPPPRSASTADEKVPVIRPRRRSSCVSLGESAAGYYGSGKMFRRPSLPCISREQMILWDWDLKQWCKPHYQNSGGGNGVDLSVLNEARNMVSDLLIDPSLPPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFYPCSEVEDPVEKGDRKLHKGLSSKPSFPTAQLRRSSGASGLLTSEHHSRWDRSGGKRPYQELSVSSHGCHLNGPFSSNLMTIPKQRSSSVSLTHHAGLRRAGALPSPSLLNSSSHVPVSAGCLTNRSPVGFLDTSDFLTKPSVTLHRSLGSVSSAADFHQYLRNSDSSLCSSCGHQILKYVSTCEPDGTDHHNEKSGEEDSTVFSKERLNIVETQEEETVKEDCRELFLEGDDHLMEEAQQPNIDQEVLLDPMLVEDYDSLIEKMSNWNFQIFELVEKMGEKSGRILSQVMYTLFQDTGLLETFKIPTQEFMNYFRALENGYRDIPYHNRVHATDVLHAVWYLTTRPIPGLQQLHNNHETETKADSDARLSSGQIAYLSSKSCCIPDKSYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLNLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSSENDRLLVCQVCIKLADINGPAKDRDLHLRWTEGIVNEFYEQGDEEATLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWIEAEEGDDTESDDDDDDDDDDDDDDDEELDSDDEETEDNLNPKPQRRKGRRRIFCQLMHHLTENHKIWKEIIEEEEKCKAEGNKLQVDNASLPQADEIQVIEEADEEEEQMFE

Target ORF information:

RefSeq Version	NM_017229.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 3B (Pde3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017229.1

ORF Insert Sequence:

ATGAGGAAGG ACGAGCGCGA GCGGGACACG CCAGCCATGA GGTCCCCGCC GCCGCCGCCG 
CCGCCGGCCA CGGCCACGGC CGCCTCGCCC CCCGAGAGCC TGCGCAACGG CTACGTGAAG 
AGCTGCGTGA GCCCGCTGCG GCAGGACCCT CCGCGCAGCT TCTTCTTCCA CCTCTGCCGC 
TTCTGCAACG TGGAGCCGCC GGCGGCCTCG CTCCGCGCCG GGGCACGCCT CTCGCTCGCC 
GCTCTGGCCG CCTTTGTCCT GGCCGCGCTG CTGGGCGCGG GGCCCGAGCG CTGGGCGGCC 
GCGGCGACCG GGCTTCGGAC GCTGCTGAGC GCTTGCTCGC TCAGCCTCAG CCCGCTCTTC 
AGCATCGCCT GTGCCTTCTT CTTCCTCACC TGCTTCCTGA CGCGCGCGCA GCGCGGCCCG 
GACCGCGGCG CCGGCTCCTG GTGGCTGCTG GCGCTGCCTG CCTGCTGCTA CCTGGGCGAC 
TTCGCGGCGT GGCAGTGGTG GTCGTGGCTG CGCGGGGAGC CGGCGGCGGC GGCGGCCGGC 
CGGCTCTGCC TGGTGCTGAG CTGCGTGGGG CTGCTGACGC TCGCGCCTCG CGTGAGGCTG 
CGGCACGGAG TCCTGGTGCT GCTCTTCGCC GGCCTGGTGT GGTGGGTGTC CTTCTCCGGC 
CTCGGGGCTC TGCCGCCCGC GCTCAGGCCT CTGCTGTCGT GCCTGGTCGG GGGCGCGGGA 
TGCCTGCTAG CCCTGGGCTT GGACCACTTC TTTCACGTCC GGGGAGCCTC ACCTCCCCCG 
CGCTCGGCGA GTACCGCGGA TGAAAAAGTG CCTGTGATCA GACCCCGGAG GAGGTCCAGC 
TGCGTGTCGC TGGGAGAAAG CGCGGCCGGT TACTATGGCA GTGGCAAGAT GTTCAGGAGA 
CCGTCGTTGC CTTGTATTTC TCGAGAACAG ATGATTCTCT GGGACTGGGA CTTGAAGCAG 
TGGTGTAAAC CTCATTACCA GAATTCTGGA GGTGGAAATG GAGTTGATCT TTCAGTGCTA 
AATGAAGCTC GCAATATGGT GTCAGACCTT CTGATTGACC CAAGCCTTCC CCCACAAGTC 
ATTTCTTCTT TGCGGAGTAT CAGTAGCTTG ATGGGTGCTT TCTCAGGTTC CTGTAGGCCA 
AAGATTAATT CTTTCACACC ATTTCCTGGA TTTTATCCCT GTTCTGAAGT AGAAGATCCA 
GTAGAGAAAG GCGATCGAAA ACTTCACAAG GGGTTGAGTA GCAAACCCAG TTTCCCGACT 
GCACAGCTGA GGAGGAGCTC AGGAGCGTCA GGATTGCTGA CCAGTGAGCA CCACTCACGG 
TGGGATCGCA GCGGTGGCAA AAGGCCTTAC CAAGAACTCA GTGTTTCAAG TCATGGATGC 
CACCTAAATG GGCCTTTTAG TTCAAATCTT ATGACAATTC CAAAGCAGAG GTCATCATCT 
GTGTCGCTGA CGCACCATGC AGGTCTGAGA AGAGCTGGTG CTTTGCCCAG CCCCAGTCTT 
CTGAATTCTT CAAGCCATGT ACCCGTGTCT GCTGGCTGTC TAACTAATCG ATCACCCGTA 
GGATTTCTTG ATACCAGTGA TTTTCTTACT AAGCCAAGTG TTACTTTACA TAGATCACTG 
GGCAGTGTAT CCAGTGCAGC AGATTTCCAT CAGTACCTTA GGAACTCTGA CAGCAGTCTG 
TGTAGCAGCT GTGGACACCA AATACTCAAG TATGTTTCGA CATGTGAACC TGATGGTACA 
GACCACCACA ATGAAAAATC AGGTGAAGAA GACAGCACTG TTTTCTCAAA AGAACGATTG 
AACATTGTGG AAACTCAAGA AGAAGAGACA GTGAAGGAAG ACTGCAGGGA ATTATTTTTG 
GAAGGTGATG ATCACCTAAT GGAAGAGGCC CAGCAACCGA ATATTGACCA GGAAGTGTTA 
TTGGACCCGA TGTTAGTGGA AGATTATGAT TCATTAATAG AAAAGATGAG CAACTGGAAC 
TTTCAGATTT TTGAGCTTGT AGAAAAAATG GGAGAAAAAT CAGGAAGGAT TCTCAGTCAG 
GTTATGTATA CTTTATTTCA AGATACTGGT TTATTGGAAA CATTTAAAAT TCCCACTCAA 
GAATTTATGA ATTATTTTCG TGCACTAGAA AATGGCTACC GAGATATTCC ATATCACAAT 
CGTGTGCATG CCACAGATGT CCTACATGCT GTTTGGTATT TGACAACACG ACCAATTCCT 
GGCTTACAGC AGCTCCATAA TAACCATGAA ACAGAAACCA AAGCAGATTC AGATGCTAGA 
CTTAGTTCTG GACAGATTGC TTACCTTTCT TCGAAGAGTT GCTGTATTCC AGATAAGAGT 
TATGGCTGCC TGTCTTCAAA CATTCCTGCG TTAGAACTGA TGGCTTTATA TGTGGCAGCT 
GCCATGCACG ATTATGATCA CCCAGGAAGA ACAAATGCAT TCCTAGTGGC TACAAATGCA 
CCTCAGGCAG TTTTATACAA TGACAGATCT GTTCTAGAAA ATCATCATGC CGCATCAGCG 
TGGAATCTGT ATCTTTCTCG CCCAGAATAC AACTTCCTCC TTAACCTTGA TCACATGGAA 
TTCAAGCGTT TTCGATTTTT AGTCATAGAA GCAATCCTTG CTACAGATCT CAAAAAACAT 
TTTGATTTCC TTGCTGAATT CAATGCCAAG GCCAATGATG TAAATAGTAA CGGTATAGAA 
TGGAGCAGTG AAAACGATCG ACTCTTAGTC TGCCAGGTGT GCATCAAATT AGCAGACATC 
AACGGCCCAG CAAAAGATCG GGATCTTCAT TTGAGATGGA CAGAAGGCAT TGTGAATGAG 
TTTTATGAGC AGGGAGATGA AGAAGCAACC CTGGGTCTAC CTATTAGTCC CTTCATGGAT 
CGTTCTTCTC CTCAACTAGC AAAACTCCAA GAGTCATTTA TCACCCACAT TGTGGGCCCC 
CTGTGCAACT CCTATGATGC TGCTGGCTTG CTGCCTGGTC AGTGGATAGA AGCAGAAGAG 
GGTGACGATA CAGAAAGTGA TGATGATGAT GATGATGATG ATGATGATGA TGATGATGAT 
GATGAAGAGT TAGATTCAGA TGATGAAGAA ACAGAAGACA ATCTAAATCC TAAACCACAA 
AGAAGGAAAG GCAGGCGGCG AATATTTTGC CAACTAATGC ACCACCTCAC TGAAAACCAC 
AAGATCTGGA AGGAAATCAT AGAAGAGGAA GAAAAATGTA AAGCTGAGGG GAACAAACTG 
CAGGTGGATA ATGCCTCCTT ACCTCAGGCA GATGAGATTC AGGTCATTGA AGAAGCAGAT 
GAAGAGGAAG AACAAATGTT TGAATGA