Pde3b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde3b
Entrez Gene ID	18576
Full Name	phosphodiesterase 3B, cGMP-inhibited
Synonyms	9830102A01Rik,AI847709
General protein information	Preferred Names phosphodiesterase 3B, cGMP-inhibited Names cGMP-inhibited 3',5'-cyclic phosphodiesterase B CGI-PDE B CGIPDE1 cyclic GMP-inhibited phosphodiesterase B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 F1\|7 59.46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011055.2	NP_035185.2	cGMP-inhibited 3',5'-cyclic phosphodiesterase B
XM_006507438.4	XP_006507501.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X1
XM_006507438.3	XP_006507501.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDE3B	NP_000913.2
Pan troglodytes (chimpanzee)	PDE3B	XP_003312976.1
	PDE3B	XP_001092891.1
Canis lupus familiaris (dog)	PDE3B	XP_854529.2
Bos taurus (cattle)	PDE3B	XP_002693091.1
Mus musculus (house mouse)	Pde3b	NP_035185.2
Rattus norvegicus (Norway rat)	Pde3b	NP_058925.1
Gallus gallus (chicken)	PDE3B	NP_001026353.1
Danio rerio (zebrafish)	pde3b	XP_691883.4

Gene Ontology
Bibliography

Related articles in PubMed

White to beige conversion in PDE3B KO adipose tissue through activation of AMPK signaling and mitochondrial function.
Chung YW, Ahmad F, Tang Y, Hockman SC, Kee HJ, Berger K, Guirguis E, Choi YH, Schimel DM, Aponte AM, Park S, Degerman E, Manganiello VC
Scientific reports740445(2017 Jan)

Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels.
Ru? L, Ba?ez-Coronel M, Creus-Muncunill J, Giralt A, Alcal?-Vida R, Mentxaka G, Kagerbauer B, Zome?o-Abell?n MT, Aranda Z, Venturi V, P?rez-Navarro E, Estivill X, Mart? E
The Journal of clinical investigation126(11)4319-4330(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Insulin induction of hypothalamic phosphodiesterase 3B activity and the reversal of the anorectic and body weight-reducing effects and stimulatory effect of insulin on hypothalamic proopiomelanocortin gene expression by cilostamide suggest that activation of phosphodiesterase 3B is a novel mechanism of insulin signaling in the hypothalamus.
Title: Hypothalamic Phosphodiesterase 3B Pathway Mediates Anorectic and Body Weight-Reducing Effects of Insulin in Male Mice.

Study concludes that CUGBP1 is a critical regulator of insulin secretion via activating PDE3B.
Title: RNA-binding protein CUGBP1 regulates insulin secretion via activation of phosphodiesterase 3B in mice.

These results suggest the possibility for a direct role for the PDE3B pathway in mediating leptin action in the hypothalamus.
Title: Leptin receptor expressing neurons express phosphodiesterase-3B (PDE3B) and leptin induces STAT3 activation in PDE3B neurons in the mouse hypothalamus.

Lack of PDE3B in brown adipocytes impairs the action of insulin on lipolysis.
Title: The Role of PDE3B Phosphorylation in the Inhibition of Lipolysis by Insulin.

these findings indicate that PDE3B deletion confers cardioprotective effects because of cAMP/PKA-induced preconditioning, which is associated with the accumulation of proteins with cardioprotective function in ICEFs.
Title: Targeted disruption of PDE3B, but not PDE3A, protects murine heart from ischemia/reperfusion injury.

The following Pde3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu39362	XM_006507438.4 Latest version!	Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu07381	NM_011055.2 Latest version!	Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OMu39362	XM_006507438.3	Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde3b ( XM_006507438.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507501.1 Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39362
Clone ID Related Accession (Same CDS sequence)	XM_006507438.4 , XM_006507438.3
Accession Version	XM_006507438.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507438.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAG ACGAGCGCGA GCGGGACGCG CCAGCCATGA GGTCCCCGCC GCCGCCGCCA 
GCCTCGGCCG CCTCGCCCCC CGAGAGCCTG CGCAACGGCT ACGTGAAGAG CTGCGTGAGC 
CCGCTGCGGC AGGACCCTCC GCGCAGCTTC TTCTTCCACC TCTGCCGCTT CTGCAACGTG 
GAGCCGCCGG CGGCCTCGCT CCGCGCCGGG GCACGCCTCT CGCTCGGCGT CCTGGCCGCC 
TTTGTCCTGG CCGCGCTGCT GGGCGCGAGG CCCGAGCGCT GGGCGGCCGC GGCAGCCGGG 
CTCCGGACGC TGCTGAGCGC CTGCTCGCTC AGCCTCAGCC CGCTCTTCAG CATCGCCTGT 
GCCTTCTTCT TCCTCACCTG TTTCCTGACG CGCGCGCAGC GCGGCCCGGG CCGCGGCGCC 
GGCTCCTGGT GGCTGCTGGC GCTGCCCGCC TGCTGCTACC TGGGCGACTT CGCGGCGTGG 
CAGTGGTGGT CGTGGCTGCG TGGGGAGCCG GCGGCGGCGG GCCGGCTCTG CCTGGTGCTG 
AGCTGCGTGG GGCTGCTGAC GCTCGCGCCC CGCGTGAGGC TGCGGCACGG CGTCCTGGTG 
CTGCTCTTCG CCGGCCTGGT GTGGTGGGTG TCCTTCTCCG GCCTCGGGGC TCTGCCGCCC 
GCGCTCAGGC CTCTGCTGTC GTGCCTGGTC GGGGGCGCGG GATGCCTGCT AGCCCTGGGC 
TTGGACCACT TCTTTCACGT CCGGGGAGCC TCCCCTCCGC CGCGCTCGGC GAGTACCGCG 
GAGGAAAAAG TGCCTGTGAT CAGACCCCGG AGGAGGTCCA GCTGCGTGTC GCTGGGAGAA 
AGCGCAGCCG GTTACTATGG CAGTGGCAAG ATGTTCAGGA GACCGTCGTT GCCTTGTATT 
TCCCGAGAAC AGATGATCCT CTGGGACTGG GACTTGAAGC AGTGGTGTAA ACCTCATTAC 
CAGAATTCTG GAGGTGGAAA TGGAGTTGAT CTTTCAGTGC TAAATGAAGC TCGCAATATG 
GTGTCAGACC TGCTGATTGA CCCAAGCCTT CCCCCACAAG TCATTTCTTC TCTGCGGAGT 
ATCAGTAGCT TGATGGGTGC TTTCTCAGGT TCCTGTAGGC CAAAGATTAA TTCTTTTACA 
CCATTTCCTG GATTTTATCC CTGCTCTGAA GTAGAAGATC CAGTTGAGAA AGGAGATCGA 
AAACTTCACA AGGGATTGAG TGGCAGAACC AGTTTCCCAA CTCCACAGCT GAGGAGGAGC 
TCTGGAGCCT CATCGTTGCT GACTAATGAG CACTGTTCAA GTCATGGATG CCACCTAAAT 
GGGCCTTTTA GTTCAAATCT TTTTACAATT CCAAAGCAGA GGTCATCGTC TGTGTCACTG 
ACGCACCATG CAGGTCTGAG AAGAGCTGGT GCTTTGCCCA GCCACAGTCT TCTGAATTCT 
TCAAGCCATG TACCAGTGTC TGCTGGCTCT CTAACTAATC GATCACCCAT AGGATTTCCT 
GATACCACTG ATTTTCTTAC TAAGCCAAAT ATTATTTTAC ATAGATCACT GGGCAGTGTA 
TCAAGTGCAG CAGATTTCCA TCAGTACCTT AGGAACTCTG ACAGCAATCT GTGTAGCAGC 
TGTGGACACC AAATACTCAA ATATGTTTCA ACATGTGAAC CCGATGGTAC AGACCACCCC 
AGTGAAAAAT CAGGTGAAGA AGACAGCAGT GTTTTCTCAA AAGAACCATT GAACATTGTG 
GAAACCCAAG AAGAAGAGAC CATGAAGAAA GCCTGCAGGG AGTTATTTTT GGAAGGTGAT 
AGTCACCTGA TGGAAGAGGC ACAGCAACCA AATATCGATC AGGAAGTGTC ACTGGATCCA 
ATGTTAGTAG AAGATTATGA TTCATTAATA GAAAAGATGA ACAACTGGAA TTTTCAGATT 
TTTGAGCTTG TAGAAAAGAT GGGAGAAAAA TCAGGAAGGA TTCTCAGTCA GGTTATGTAT 
ACTTTATTTC AAGATACTGG TTTATTGGAA ACATTTAAAA TTCCCACTCA AGAATTTATG 
AATTATTTTC GTGCCTTAGA AAATGGCTAC CGGGACATTC CATATCACAA TCGTGTGCAT 
GCCACAGATG TCCTACATGC TGTTTGGTAT TTGACAACCC GACCAATTCC TGGCTTACCT 
CAGATCCATA ATAACCATGA AACGGAAACC AAAGCAGATT CAGATGGTAG ACTTGGTTCT 
GGACAGATTG CTTACATTTC TTCGAAGAGT TGCTGTATTC CAGATATGAG TTATGGCTGC 
CTATCTTCAA ACATCCCTGC ATTAGAATTG ATGGCTTTGT ATGTGGCAGC TGCCATGCAC 
GATTACGATC ACCCAGGAAG AACAAATGCG TTTCTAGTGG CTACAAATGC ACCTCAGGCA 
GTTTTATACA ATGACAGATC TGTTCTAGAG AATCATCATG CTGCATCAGC TTGGAATCTG 
TATCTTTCTC GCCCAGAGTA CAACTTCCTC CTTAACCTTG ATCACATGGA ATTCAAGCGG 
TTTCGATTTT TAGTTATAGA AGCAATCCTT GCTACAGATC TCAAAAAACA TTTTGATTTC 
CTTGCAGAAT TCAATGCCAA GGCCAATGAT GTAAATAGTA ACGGTATAGA ATGGAGCAGT 
GAAAACGATC GCCTCTTGGT CTGCCAGGTG TGCATCAAAT TAGCAGATAT CAATGGCCCA 
GCAAAAGATC GGGACCTACA TTTGAGATGG ACAGAAGGCA TTGTGAATGA ATTTTATGAG 
CAGGGAGATG AAGAAGCAAC CCTGGGTCTA CCTATTAGTC CCTTCATGGA TCGTTCTTCT 
CCTCAACTAG CAAAACTCCA AGAATCTTTT ATCACCCACA TTGTGGGCCC CCTGTGCAAC 
TCCTATGATG CTGCTGGCTT GCTGCCGGGT CAGTGGATAG AAACAGAAGA GGGTGATGAT 
ACAGAAAGTG ATGATGATGA TGATGATGAT GATGGTGGTG GTGAAGAATT AGATTCAGAT 
GATGAAGAAA CAGAAGACAA TCTAAATCCT AAACCACAAA GAAGGAAAGG CAGGCGGCGA 
ATATTTTGCC AACTAATGCA CCACCTCACT GAAAACCACA AGATATGGAA GGAAATCATA 
GAAGAAGAAG AAGAAAAATG TAAAGCTGAG GGGAACAAAC TGCAGGTGGA TAATGCCTCC 
TTACCTCAGG CAGATGAGAT TCAGGTTATT GAAGAAGCAG ATGAAGAGGA AGAACAAATG 
TTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507501.1
CDS	273..3521
Translation	MRKDERERDAPAMRSPPPPPASAASPPESLRNGYVKSCVSPLRQDPPRSFFFHLCRFCNVEPPAASLRAGARLSLGVLAAFVLAALLGARPERWAAAAAGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQRGPGRGAGSWWLLALPACCYLGDFAAWQWWSWLRGEPAAAGRLCLVLSCVGLLTLAPRVRLRHGVLVLLFAGLVWWVSFSGLGALPPALRPLLSCLVGGAGCLLALGLDHFFHVRGASPPPRSASTAEEKVPVIRPRRRSSCVSLGESAAGYYGSGKMFRRPSLPCISREQMILWDWDLKQWCKPHYQNSGGGNGVDLSVLNEARNMVSDLLIDPSLPPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFYPCSEVEDPVEKGDRKLHKGLSGRTSFPTPQLRRSSGASSLLTNEHCSSHGCHLNGPFSSNLFTIPKQRSSSVSLTHHAGLRRAGALPSHSLLNSSSHVPVSAGSLTNRSPIGFPDTTDFLTKPNIILHRSLGSVSSAADFHQYLRNSDSNLCSSCGHQILKYVSTCEPDGTDHPSEKSGEEDSSVFSKEPLNIVETQEEETMKKACRELFLEGDSHLMEEAQQPNIDQEVSLDPMLVEDYDSLIEKMNNWNFQIFELVEKMGEKSGRILSQVMYTLFQDTGLLETFKIPTQEFMNYFRALENGYRDIPYHNRVHATDVLHAVWYLTTRPIPGLPQIHNNHETETKADSDGRLGSGQIAYISSKSCCIPDMSYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLNLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSSENDRLLVCQVCIKLADINGPAKDRDLHLRWTEGIVNEFYEQGDEEATLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWIETEEGDDTESDDDDDDDDGGGEELDSDDEETEDNLNPKPQRRKGRRRIFCQLMHHLTENHKIWKEIIEEEEEKCKAEGNKLQVDNASLPQADEIQVIEEADEEEEQMFE

Target ORF information:

RefSeq Version	XM_006507438.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507438.4

ORF Insert Sequence:

ATGAGGAAAG ACGAGCGCGA GCGGGACGCG CCAGCCATGA GGTCCCCGCC GCCGCCGCCA 
GCCTCGGCCG CCTCGCCCCC CGAGAGCCTG CGCAACGGCT ACGTGAAGAG CTGCGTGAGC 
CCGCTGCGGC AGGACCCTCC GCGCAGCTTC TTCTTCCACC TCTGCCGCTT CTGCAACGTG 
GAGCCGCCGG CGGCCTCGCT CCGCGCCGGG GCACGCCTCT CGCTCGGCGT CCTGGCCGCC 
TTTGTCCTGG CCGCGCTGCT GGGCGCGAGG CCCGAGCGCT GGGCGGCCGC GGCAGCCGGG 
CTCCGGACGC TGCTGAGCGC CTGCTCGCTC AGCCTCAGCC CGCTCTTCAG CATCGCCTGT 
GCCTTCTTCT TCCTCACCTG TTTCCTGACG CGCGCGCAGC GCGGCCCGGG CCGCGGCGCC 
GGCTCCTGGT GGCTGCTGGC GCTGCCCGCC TGCTGCTACC TGGGCGACTT CGCGGCGTGG 
CAGTGGTGGT CGTGGCTGCG TGGGGAGCCG GCGGCGGCGG GCCGGCTCTG CCTGGTGCTG 
AGCTGCGTGG GGCTGCTGAC GCTCGCGCCC CGCGTGAGGC TGCGGCACGG CGTCCTGGTG 
CTGCTCTTCG CCGGCCTGGT GTGGTGGGTG TCCTTCTCCG GCCTCGGGGC TCTGCCGCCC 
GCGCTCAGGC CTCTGCTGTC GTGCCTGGTC GGGGGCGCGG GATGCCTGCT AGCCCTGGGC 
TTGGACCACT TCTTTCACGT CCGGGGAGCC TCCCCTCCGC CGCGCTCGGC GAGTACCGCG 
GAGGAAAAAG TGCCTGTGAT CAGACCCCGG AGGAGGTCCA GCTGCGTGTC GCTGGGAGAA 
AGCGCAGCCG GTTACTATGG CAGTGGCAAG ATGTTCAGGA GACCGTCGTT GCCTTGTATT 
TCCCGAGAAC AGATGATCCT CTGGGACTGG GACTTGAAGC AGTGGTGTAA ACCTCATTAC 
CAGAATTCTG GAGGTGGAAA TGGAGTTGAT CTTTCAGTGC TAAATGAAGC TCGCAATATG 
GTGTCAGACC TGCTGATTGA CCCAAGCCTT CCCCCACAAG TCATTTCTTC TCTGCGGAGT 
ATCAGTAGCT TGATGGGTGC TTTCTCAGGT TCCTGTAGGC CAAAGATTAA TTCTTTTACA 
CCATTTCCTG GATTTTATCC CTGCTCTGAA GTAGAAGATC CAGTTGAGAA AGGAGATCGA 
AAACTTCACA AGGGATTGAG TGGCAGAACC AGTTTCCCAA CTCCACAGCT GAGGAGGAGC 
TCTGGAGCCT CATCGTTGCT GACTAATGAG CACTGTTCAA GTCATGGATG CCACCTAAAT 
GGGCCTTTTA GTTCAAATCT TTTTACAATT CCAAAGCAGA GGTCATCGTC TGTGTCACTG 
ACGCACCATG CAGGTCTGAG AAGAGCTGGT GCTTTGCCCA GCCACAGTCT TCTGAATTCT 
TCAAGCCATG TACCAGTGTC TGCTGGCTCT CTAACTAATC GATCACCCAT AGGATTTCCT 
GATACCACTG ATTTTCTTAC TAAGCCAAAT ATTATTTTAC ATAGATCACT GGGCAGTGTA 
TCAAGTGCAG CAGATTTCCA TCAGTACCTT AGGAACTCTG ACAGCAATCT GTGTAGCAGC 
TGTGGACACC AAATACTCAA ATATGTTTCA ACATGTGAAC CCGATGGTAC AGACCACCCC 
AGTGAAAAAT CAGGTGAAGA AGACAGCAGT GTTTTCTCAA AAGAACCATT GAACATTGTG 
GAAACCCAAG AAGAAGAGAC CATGAAGAAA GCCTGCAGGG AGTTATTTTT GGAAGGTGAT 
AGTCACCTGA TGGAAGAGGC ACAGCAACCA AATATCGATC AGGAAGTGTC ACTGGATCCA 
ATGTTAGTAG AAGATTATGA TTCATTAATA GAAAAGATGA ACAACTGGAA TTTTCAGATT 
TTTGAGCTTG TAGAAAAGAT GGGAGAAAAA TCAGGAAGGA TTCTCAGTCA GGTTATGTAT 
ACTTTATTTC AAGATACTGG TTTATTGGAA ACATTTAAAA TTCCCACTCA AGAATTTATG 
AATTATTTTC GTGCCTTAGA AAATGGCTAC CGGGACATTC CATATCACAA TCGTGTGCAT 
GCCACAGATG TCCTACATGC TGTTTGGTAT TTGACAACCC GACCAATTCC TGGCTTACCT 
CAGATCCATA ATAACCATGA AACGGAAACC AAAGCAGATT CAGATGGTAG ACTTGGTTCT 
GGACAGATTG CTTACATTTC TTCGAAGAGT TGCTGTATTC CAGATATGAG TTATGGCTGC 
CTATCTTCAA ACATCCCTGC ATTAGAATTG ATGGCTTTGT ATGTGGCAGC TGCCATGCAC 
GATTACGATC ACCCAGGAAG AACAAATGCG TTTCTAGTGG CTACAAATGC ACCTCAGGCA 
GTTTTATACA ATGACAGATC TGTTCTAGAG AATCATCATG CTGCATCAGC TTGGAATCTG 
TATCTTTCTC GCCCAGAGTA CAACTTCCTC CTTAACCTTG ATCACATGGA ATTCAAGCGG 
TTTCGATTTT TAGTTATAGA AGCAATCCTT GCTACAGATC TCAAAAAACA TTTTGATTTC 
CTTGCAGAAT TCAATGCCAA GGCCAATGAT GTAAATAGTA ACGGTATAGA ATGGAGCAGT 
GAAAACGATC GCCTCTTGGT CTGCCAGGTG TGCATCAAAT TAGCAGATAT CAATGGCCCA 
GCAAAAGATC GGGACCTACA TTTGAGATGG ACAGAAGGCA TTGTGAATGA ATTTTATGAG 
CAGGGAGATG AAGAAGCAAC CCTGGGTCTA CCTATTAGTC CCTTCATGGA TCGTTCTTCT 
CCTCAACTAG CAAAACTCCA AGAATCTTTT ATCACCCACA TTGTGGGCCC CCTGTGCAAC 
TCCTATGATG CTGCTGGCTT GCTGCCGGGT CAGTGGATAG AAACAGAAGA GGGTGATGAT 
ACAGAAAGTG ATGATGATGA TGATGATGAT GATGGTGGTG GTGAAGAATT AGATTCAGAT 
GATGAAGAAA CAGAAGACAA TCTAAATCCT AAACCACAAA GAAGGAAAGG CAGGCGGCGA 
ATATTTTGCC AACTAATGCA CCACCTCACT GAAAACCACA AGATATGGAA GGAAATCATA 
GAAGAAGAAG AAGAAAAATG TAAAGCTGAG GGGAACAAAC TGCAGGTGGA TAATGCCTCC 
TTACCTCAGG CAGATGAGAT TCAGGTTATT GAAGAAGCAG ATGAAGAGGA AGAACAAATG 
TTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde3b ( NM_011055.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035185.2 Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), mRNA.

Price & Availability↑

CloneID	OMu07381
Clone ID Related Accession (Same CDS sequence)	NM_011055.2
Accession Version	NM_011055.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB627812.1, AJ132271.1, CJ058099.1, BY745829.1, BU613166.1, BB663310.1, AK142889.1, AV270888.2 and CJ299201.1. On Aug 23, 2006 this sequence version replaced NM_011055.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF547435.1, AJ132271.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAAAG ACGAGCGCGA GCGGGACGCG CCAGCCATGA GGTCCCCGCC GCCGCCGCCA 
GCCTCGGCCG CCTCGCCCCC CGAGAGCCTG CGCAACGGCT ACGTGAAGAG CTGCGTGAGC 
CCGCTGCGGC AGGACCCTCC GCGCAGCTTC TTCTTCCACC TCTGCCGCTT CTGCAACGTG 
GAGCCGCCGG CGGCCTCGCT CCGCGCCGGG GCACGCCTCT CGCTCGGCGT CCTGGCCGCC 
TTTGTCCTGG CCGCGCTGCT GGGCGCGAGG CCCGAGCGCT GGGCGGCCGC GGCAGCCGGG 
CTCCGGACGC TGCTGAGCGC CTGCTCGCTC AGCCTCAGCC CGCTCTTCAG CATCGCCTGT 
GCCTTCTTCT TCCTCACCTG TTTCCTGACG CGCGCGCAGC GCGGCCCGGG CCGCGGCGCC 
GGCTCCTGGT GGCTGCTGGC GCTGCCCGCC TGCTGCTACC TGGGCGACTT CGCGGCGTGG 
CAGTGGTGGT CGTGGCTGCG TGGGGAGCCG GCGGCGGCGG GCCGGCTCTG CCTGGTGCTG 
AGCTGCGTGG GGCTGCTGAC GCTCGCGCCC CGCGTGAGGC TGCGGCACGG CGTCCTGGTG 
CTGCTCTTCG CCGGCCTGGT GTGGTGGGTG TCCTTCTCCG GCCTCGGGGC TCTGCCGCCC 
GCGCTCAGGC CTCTGCTGTC GTGCCTGGTC GGGGGCGCGG GATGCCTGCT AGCCCTGGGC 
TTGGACCACT TCTTTCACGT CCGGGGAGCC TCCCCTCCGC CGCGCTCGGC GAGTACCGCG 
GAGGAAAAAG TGCCTGTGAT CAGACCCCGG AGGAGGTCCA GCTGCGTGTC GCTGGGAGAA 
AGCGCAGCCG GTTACTATGG CAGTGGCAAG ATGTTCAGGA GACCGTCGTT GCCTTGTATT 
TCCCGAGAAC AGATGATCCT CTGGGACTGG GACTTGAAGC AGTGGTGTAA ACCTCATTAC 
CAGAATTCTG GAGGTGGAAA TGGAGTTGAT CTTTCAGTGC TAAATGAAGC TCGCAATATG 
GTGTCAGACC TGCTGATTGA CCCAAGCCTT CCCCCACAAG TCATTTCTTC TCTGCGGAGT 
ATCAGTAGCT TGATGGGTGC TTTCTCAGGT TCCTGTAGGC CAAAGATTAA TTCTTTTACA 
CCATTTCCTG GATTTTATCC CTGCTCTGAA GTAGAAGATC CAGTTGAGAA AGGAGATCGA 
AAACTTCACA AGGGATTGAG TGGCAGAACC AGTTTCCCAA CTCCACAGCT GAGGAGGAGC 
TCTGGAGCCT CATCGTTGCT GACTAATGAG CACTGTTCAA GGTGGGATCG CAGCAGTGGT 
AAGAGGTCTT ACCAAGAACT CAGTGTTTCA AGTCATGGAT GCCACCTAAA TGGGCCTTTT 
AGTTCAAATC TTTTTACAAT TCCAAAGCAG AGGTCATCGT CTGTGTCACT GACGCACCAT 
GCAGGTCTGA GAAGAGCTGG TGCTTTGCCC AGCCACAGTC TTCTGAATTC TTCAAGCCAT 
GTACCAGTGT CTGCTGGCTC TCTAACTAAT CGATCACCCA TAGGATTTCC TGATACCACT 
GATTTTCTTA CTAAGCCAAA TATTATTTTA CATAGATCAC TGGGCAGTGT ATCAAGTGCA 
GCAGATTTCC ATCAGTACCT TAGGAACTCT GACAGCAATC TGTGTAGCAG CTGTGGACAC 
CAAATACTCA AATATGTTTC AACATGTGAA CCCGATGGTA CAGACCACCC CAGTGAAAAA 
TCAGGTGAAG AAGACAGCAG TGTTTTCTCA AAAGAACCAT TGAACATTGT GGAAACCCAA 
GAAGAAGAGA CCATGAAGAA AGCCTGCAGG GAGTTATTTT TGGAAGGTGA TAGTCACCTG 
ATGGAAGAGG CACAGCAACC AAATATCGAT CAGGAAGTGT CACTGGATCC AATGTTAGTA 
GAAGATTATG ATTCATTAAT AGAAAAGATG AACAACTGGA ATTTTCAGAT TTTTGAGCTT 
GTAGAAAAGA TGGGAGAAAA ATCAGGAAGG ATTCTCAGTC AGGTTATGTA TACTTTATTT 
CAAGATACTG GTTTATTGGA AACATTTAAA ATTCCCACTC AAGAATTTAT GAATTATTTT 
CGTGCCTTAG AAAATGGCTA CCGGGACATT CCATATCACA ATCGTGTGCA TGCCACAGAT 
GTCCTACATG CTGTTTGGTA TTTGACAACC CGACCAATTC CTGGCTTACC TCAGATCCAT 
AATAACCATG AAACGGAAAC CAAAGCAGAT TCAGATGGTA GACTTGGTTC TGGACAGATT 
GCTTACATTT CTTCGAAGAG TTGCTGTATT CCAGATATGA GTTATGGCTG CCTATCTTCA 
AACATCCCTG CATTAGAATT GATGGCTTTG TATGTGGCAG CTGCCATGCA CGATTACGAT 
CACCCAGGAA GAACAAATGC GTTTCTAGTG GCTACAAATG CACCTCAGGC AGTTTTATAC 
AATGACAGAT CTGTTCTAGA GAATCATCAT GCTGCATCAG CTTGGAATCT GTATCTTTCT 
CGCCCAGAGT ACAACTTCCT CCTTAACCTT GATCACATGG AATTCAAGCG GTTTCGATTT 
TTAGTTATAG AAGCAATCCT TGCTACAGAT CTCAAAAAAC ATTTTGATTT CCTTGCAGAA 
TTCAATGCCA AGGCCAATGA TGTAAATAGT AACGGTATAG AATGGAGCAG TGAAAACGAT 
CGCCTCTTGG TCTGCCAGGT GTGCATCAAA TTAGCAGATA TCAATGGCCC AGCAAAAGAT 
CGGGACCTAC ATTTGAGATG GACAGAAGGC ATTGTGAATG AATTTTATGA GCAGGGAGAT 
GAAGAAGCAA CCCTGGGTCT ACCTATTAGT CCCTTCATGG ATCGTTCTTC TCCTCAACTA 
GCAAAACTCC AAGAATCTTT TATCACCCAC ATTGTGGGCC CCCTGTGCAA CTCCTATGAT 
GCTGCTGGCT TGCTGCCGGG TCAGTGGATA GAAACAGAAG AGGGTGATGA TACAGAAAGT 
GATGATGATG ATGATGATGA TGATGGTGGT GGTGAAGAAT TAGATTCAGA TGATGAAGAA 
ACAGAAGACA ATCTAAATCC TAAACCACAA AGAAGGAAAG GCAGGCGGCG AATATTTTGC 
CAACTAATGC ACCACCTCAC TGAAAACCAC AAGATATGGA AGGAAATCAT AGAAGAAGAA 
GAAGAAAAAT GTAAAGCTGA GGGGAACAAA CTGCAGGTGG ATAATGCCTC CTTACCTCAG 
GCAGATGAGA TTCAGGTTAT TGAAGAAGCA GATGAAGAGG AAGAACAAAT GTTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035185.2
CDS	298..3597
Translation	MRKDERERDAPAMRSPPPPPASAASPPESLRNGYVKSCVSPLRQDPPRSFFFHLCRFCNVEPPAASLRAGARLSLGVLAAFVLAALLGARPERWAAAAAGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQRGPGRGAGSWWLLALPACCYLGDFAAWQWWSWLRGEPAAAGRLCLVLSCVGLLTLAPRVRLRHGVLVLLFAGLVWWVSFSGLGALPPALRPLLSCLVGGAGCLLALGLDHFFHVRGASPPPRSASTAEEKVPVIRPRRRSSCVSLGESAAGYYGSGKMFRRPSLPCISREQMILWDWDLKQWCKPHYQNSGGGNGVDLSVLNEARNMVSDLLIDPSLPPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFYPCSEVEDPVEKGDRKLHKGLSGRTSFPTPQLRRSSGASSLLTNEHCSRWDRSSGKRSYQELSVSSHGCHLNGPFSSNLFTIPKQRSSSVSLTHHAGLRRAGALPSHSLLNSSSHVPVSAGSLTNRSPIGFPDTTDFLTKPNIILHRSLGSVSSAADFHQYLRNSDSNLCSSCGHQILKYVSTCEPDGTDHPSEKSGEEDSSVFSKEPLNIVETQEEETMKKACRELFLEGDSHLMEEAQQPNIDQEVSLDPMLVEDYDSLIEKMNNWNFQIFELVEKMGEKSGRILSQVMYTLFQDTGLLETFKIPTQEFMNYFRALENGYRDIPYHNRVHATDVLHAVWYLTTRPIPGLPQIHNNHETETKADSDGRLGSGQIAYISSKSCCIPDMSYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLNLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSSENDRLLVCQVCIKLADINGPAKDRDLHLRWTEGIVNEFYEQGDEEATLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWIETEEGDDTESDDDDDDDDGGGEELDSDDEETEDNLNPKPQRRKGRRRIFCQLMHHLTENHKIWKEIIEEEEEKCKAEGNKLQVDNASLPQADEIQVIEEADEEEEQMFE

Target ORF information:

RefSeq Version	NM_011055.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011055.2

ORF Insert Sequence:

ATGAGGAAAG ACGAGCGCGA GCGGGACGCG CCAGCCATGA GGTCCCCGCC GCCGCCGCCA 
GCCTCGGCCG CCTCGCCCCC CGAGAGCCTG CGCAACGGCT ACGTGAAGAG CTGCGTGAGC 
CCGCTGCGGC AGGACCCTCC GCGCAGCTTC TTCTTCCACC TCTGCCGCTT CTGCAACGTG 
GAGCCGCCGG CGGCCTCGCT CCGCGCCGGG GCACGCCTCT CGCTCGGCGT CCTGGCCGCC 
TTTGTCCTGG CCGCGCTGCT GGGCGCGAGG CCCGAGCGCT GGGCGGCCGC GGCAGCCGGG 
CTCCGGACGC TGCTGAGCGC CTGCTCGCTC AGCCTCAGCC CGCTCTTCAG CATCGCCTGT 
GCCTTCTTCT TCCTCACCTG TTTCCTGACG CGCGCGCAGC GCGGCCCGGG CCGCGGCGCC 
GGCTCCTGGT GGCTGCTGGC GCTGCCCGCC TGCTGCTACC TGGGCGACTT CGCGGCGTGG 
CAGTGGTGGT CGTGGCTGCG TGGGGAGCCG GCGGCGGCGG GCCGGCTCTG CCTGGTGCTG 
AGCTGCGTGG GGCTGCTGAC GCTCGCGCCC CGCGTGAGGC TGCGGCACGG CGTCCTGGTG 
CTGCTCTTCG CCGGCCTGGT GTGGTGGGTG TCCTTCTCCG GCCTCGGGGC TCTGCCGCCC 
GCGCTCAGGC CTCTGCTGTC GTGCCTGGTC GGGGGCGCGG GATGCCTGCT AGCCCTGGGC 
TTGGACCACT TCTTTCACGT CCGGGGAGCC TCCCCTCCGC CGCGCTCGGC GAGTACCGCG 
GAGGAAAAAG TGCCTGTGAT CAGACCCCGG AGGAGGTCCA GCTGCGTGTC GCTGGGAGAA 
AGCGCAGCCG GTTACTATGG CAGTGGCAAG ATGTTCAGGA GACCGTCGTT GCCTTGTATT 
TCCCGAGAAC AGATGATCCT CTGGGACTGG GACTTGAAGC AGTGGTGTAA ACCTCATTAC 
CAGAATTCTG GAGGTGGAAA TGGAGTTGAT CTTTCAGTGC TAAATGAAGC TCGCAATATG 
GTGTCAGACC TGCTGATTGA CCCAAGCCTT CCCCCACAAG TCATTTCTTC TCTGCGGAGT 
ATCAGTAGCT TGATGGGTGC TTTCTCAGGT TCCTGTAGGC CAAAGATTAA TTCTTTTACA 
CCATTTCCTG GATTTTATCC CTGCTCTGAA GTAGAAGATC CAGTTGAGAA AGGAGATCGA 
AAACTTCACA AGGGATTGAG TGGCAGAACC AGTTTCCCAA CTCCACAGCT GAGGAGGAGC 
TCTGGAGCCT CATCGTTGCT GACTAATGAG CACTGTTCAA GGTGGGATCG CAGCAGTGGT 
AAGAGGTCTT ACCAAGAACT CAGTGTTTCA AGTCATGGAT GCCACCTAAA TGGGCCTTTT 
AGTTCAAATC TTTTTACAAT TCCAAAGCAG AGGTCATCGT CTGTGTCACT GACGCACCAT 
GCAGGTCTGA GAAGAGCTGG TGCTTTGCCC AGCCACAGTC TTCTGAATTC TTCAAGCCAT 
GTACCAGTGT CTGCTGGCTC TCTAACTAAT CGATCACCCA TAGGATTTCC TGATACCACT 
GATTTTCTTA CTAAGCCAAA TATTATTTTA CATAGATCAC TGGGCAGTGT ATCAAGTGCA 
GCAGATTTCC ATCAGTACCT TAGGAACTCT GACAGCAATC TGTGTAGCAG CTGTGGACAC 
CAAATACTCA AATATGTTTC AACATGTGAA CCCGATGGTA CAGACCACCC CAGTGAAAAA 
TCAGGTGAAG AAGACAGCAG TGTTTTCTCA AAAGAACCAT TGAACATTGT GGAAACCCAA 
GAAGAAGAGA CCATGAAGAA AGCCTGCAGG GAGTTATTTT TGGAAGGTGA TAGTCACCTG 
ATGGAAGAGG CACAGCAACC AAATATCGAT CAGGAAGTGT CACTGGATCC AATGTTAGTA 
GAAGATTATG ATTCATTAAT AGAAAAGATG AACAACTGGA ATTTTCAGAT TTTTGAGCTT 
GTAGAAAAGA TGGGAGAAAA ATCAGGAAGG ATTCTCAGTC AGGTTATGTA TACTTTATTT 
CAAGATACTG GTTTATTGGA AACATTTAAA ATTCCCACTC AAGAATTTAT GAATTATTTT 
CGTGCCTTAG AAAATGGCTA CCGGGACATT CCATATCACA ATCGTGTGCA TGCCACAGAT 
GTCCTACATG CTGTTTGGTA TTTGACAACC CGACCAATTC CTGGCTTACC TCAGATCCAT 
AATAACCATG AAACGGAAAC CAAAGCAGAT TCAGATGGTA GACTTGGTTC TGGACAGATT 
GCTTACATTT CTTCGAAGAG TTGCTGTATT CCAGATATGA GTTATGGCTG CCTATCTTCA 
AACATCCCTG CATTAGAATT GATGGCTTTG TATGTGGCAG CTGCCATGCA CGATTACGAT 
CACCCAGGAA GAACAAATGC GTTTCTAGTG GCTACAAATG CACCTCAGGC AGTTTTATAC 
AATGACAGAT CTGTTCTAGA GAATCATCAT GCTGCATCAG CTTGGAATCT GTATCTTTCT 
CGCCCAGAGT ACAACTTCCT CCTTAACCTT GATCACATGG AATTCAAGCG GTTTCGATTT 
TTAGTTATAG AAGCAATCCT TGCTACAGAT CTCAAAAAAC ATTTTGATTT CCTTGCAGAA 
TTCAATGCCA AGGCCAATGA TGTAAATAGT AACGGTATAG AATGGAGCAG TGAAAACGAT 
CGCCTCTTGG TCTGCCAGGT GTGCATCAAA TTAGCAGATA TCAATGGCCC AGCAAAAGAT 
CGGGACCTAC ATTTGAGATG GACAGAAGGC ATTGTGAATG AATTTTATGA GCAGGGAGAT 
GAAGAAGCAA CCCTGGGTCT ACCTATTAGT CCCTTCATGG ATCGTTCTTC TCCTCAACTA 
GCAAAACTCC AAGAATCTTT TATCACCCAC ATTGTGGGCC CCCTGTGCAA CTCCTATGAT 
GCTGCTGGCT TGCTGCCGGG TCAGTGGATA GAAACAGAAG AGGGTGATGA TACAGAAAGT 
GATGATGATG ATGATGATGA TGATGGTGGT GGTGAAGAAT TAGATTCAGA TGATGAAGAA 
ACAGAAGACA ATCTAAATCC TAAACCACAA AGAAGGAAAG GCAGGCGGCG AATATTTTGC 
CAACTAATGC ACCACCTCAC TGAAAACCAC AAGATATGGA AGGAAATCAT AGAAGAAGAA 
GAAGAAAAAT GTAAAGCTGA GGGGAACAAA CTGCAGGTGG ATAATGCCTC CTTACCTCAG 
GCAGATGAGA TTCAGGTTAT TGAAGAAGCA GATGAAGAGG AAGAACAAAT GTTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde3b ( XM_006507438.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507501.1 Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), transcript variant X1, mRNA.

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CloneID	OMu39362
Clone ID Related Accession (Same CDS sequence)	XM_006507438.4 , XM_006507438.3
Accession Version	XM_006507438.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAAAG ACGAGCGCGA GCGGGACGCG CCAGCCATGA GGTCCCCGCC GCCGCCGCCA 
GCCTCGGCCG CCTCGCCCCC CGAGAGCCTG CGCAACGGCT ACGTGAAGAG CTGCGTGAGC 
CCGCTGCGGC AGGACCCTCC GCGCAGCTTC TTCTTCCACC TCTGCCGCTT CTGCAACGTG 
GAGCCGCCGG CGGCCTCGCT CCGCGCCGGG GCACGCCTCT CGCTCGGCGT CCTGGCCGCC 
TTTGTCCTGG CCGCGCTGCT GGGCGCGAGG CCCGAGCGCT GGGCGGCCGC GGCAGCCGGG 
CTCCGGACGC TGCTGAGCGC CTGCTCGCTC AGCCTCAGCC CGCTCTTCAG CATCGCCTGT 
GCCTTCTTCT TCCTCACCTG TTTCCTGACG CGCGCGCAGC GCGGCCCGGG CCGCGGCGCC 
GGCTCCTGGT GGCTGCTGGC GCTGCCCGCC TGCTGCTACC TGGGCGACTT CGCGGCGTGG 
CAGTGGTGGT CGTGGCTGCG TGGGGAGCCG GCGGCGGCGG GCCGGCTCTG CCTGGTGCTG 
AGCTGCGTGG GGCTGCTGAC GCTCGCGCCC CGCGTGAGGC TGCGGCACGG CGTCCTGGTG 
CTGCTCTTCG CCGGCCTGGT GTGGTGGGTG TCCTTCTCCG GCCTCGGGGC TCTGCCGCCC 
GCGCTCAGGC CTCTGCTGTC GTGCCTGGTC GGGGGCGCGG GATGCCTGCT AGCCCTGGGC 
TTGGACCACT TCTTTCACGT CCGGGGAGCC TCCCCTCCGC CGCGCTCGGC GAGTACCGCG 
GAGGAAAAAG TGCCTGTGAT CAGACCCCGG AGGAGGTCCA GCTGCGTGTC GCTGGGAGAA 
AGCGCAGCCG GTTACTATGG CAGTGGCAAG ATGTTCAGGA GACCGTCGTT GCCTTGTATT 
TCCCGAGAAC AGATGATCCT CTGGGACTGG GACTTGAAGC AGTGGTGTAA ACCTCATTAC 
CAGAATTCTG GAGGTGGAAA TGGAGTTGAT CTTTCAGTGC TAAATGAAGC TCGCAATATG 
GTGTCAGACC TGCTGATTGA CCCAAGCCTT CCCCCACAAG TCATTTCTTC TCTGCGGAGT 
ATCAGTAGCT TGATGGGTGC TTTCTCAGGT TCCTGTAGGC CAAAGATTAA TTCTTTTACA 
CCATTTCCTG GATTTTATCC CTGCTCTGAA GTAGAAGATC CAGTTGAGAA AGGAGATCGA 
AAACTTCACA AGGGATTGAG TGGCAGAACC AGTTTCCCAA CTCCACAGCT GAGGAGGAGC 
TCTGGAGCCT CATCGTTGCT GACTAATGAG CACTGTTCAA GTCATGGATG CCACCTAAAT 
GGGCCTTTTA GTTCAAATCT TTTTACAATT CCAAAGCAGA GGTCATCGTC TGTGTCACTG 
ACGCACCATG CAGGTCTGAG AAGAGCTGGT GCTTTGCCCA GCCACAGTCT TCTGAATTCT 
TCAAGCCATG TACCAGTGTC TGCTGGCTCT CTAACTAATC GATCACCCAT AGGATTTCCT 
GATACCACTG ATTTTCTTAC TAAGCCAAAT ATTATTTTAC ATAGATCACT GGGCAGTGTA 
TCAAGTGCAG CAGATTTCCA TCAGTACCTT AGGAACTCTG ACAGCAATCT GTGTAGCAGC 
TGTGGACACC AAATACTCAA ATATGTTTCA ACATGTGAAC CCGATGGTAC AGACCACCCC 
AGTGAAAAAT CAGGTGAAGA AGACAGCAGT GTTTTCTCAA AAGAACCATT GAACATTGTG 
GAAACCCAAG AAGAAGAGAC CATGAAGAAA GCCTGCAGGG AGTTATTTTT GGAAGGTGAT 
AGTCACCTGA TGGAAGAGGC ACAGCAACCA AATATCGATC AGGAAGTGTC ACTGGATCCA 
ATGTTAGTAG AAGATTATGA TTCATTAATA GAAAAGATGA ACAACTGGAA TTTTCAGATT 
TTTGAGCTTG TAGAAAAGAT GGGAGAAAAA TCAGGAAGGA TTCTCAGTCA GGTTATGTAT 
ACTTTATTTC AAGATACTGG TTTATTGGAA ACATTTAAAA TTCCCACTCA AGAATTTATG 
AATTATTTTC GTGCCTTAGA AAATGGCTAC CGGGACATTC CATATCACAA TCGTGTGCAT 
GCCACAGATG TCCTACATGC TGTTTGGTAT TTGACAACCC GACCAATTCC TGGCTTACCT 
CAGATCCATA ATAACCATGA AACGGAAACC AAAGCAGATT CAGATGGTAG ACTTGGTTCT 
GGACAGATTG CTTACATTTC TTCGAAGAGT TGCTGTATTC CAGATATGAG TTATGGCTGC 
CTATCTTCAA ACATCCCTGC ATTAGAATTG ATGGCTTTGT ATGTGGCAGC TGCCATGCAC 
GATTACGATC ACCCAGGAAG AACAAATGCG TTTCTAGTGG CTACAAATGC ACCTCAGGCA 
GTTTTATACA ATGACAGATC TGTTCTAGAG AATCATCATG CTGCATCAGC TTGGAATCTG 
TATCTTTCTC GCCCAGAGTA CAACTTCCTC CTTAACCTTG ATCACATGGA ATTCAAGCGG 
TTTCGATTTT TAGTTATAGA AGCAATCCTT GCTACAGATC TCAAAAAACA TTTTGATTTC 
CTTGCAGAAT TCAATGCCAA GGCCAATGAT GTAAATAGTA ACGGTATAGA ATGGAGCAGT 
GAAAACGATC GCCTCTTGGT CTGCCAGGTG TGCATCAAAT TAGCAGATAT CAATGGCCCA 
GCAAAAGATC GGGACCTACA TTTGAGATGG ACAGAAGGCA TTGTGAATGA ATTTTATGAG 
CAGGGAGATG AAGAAGCAAC CCTGGGTCTA CCTATTAGTC CCTTCATGGA TCGTTCTTCT 
CCTCAACTAG CAAAACTCCA AGAATCTTTT ATCACCCACA TTGTGGGCCC CCTGTGCAAC 
TCCTATGATG CTGCTGGCTT GCTGCCGGGT CAGTGGATAG AAACAGAAGA GGGTGATGAT 
ACAGAAAGTG ATGATGATGA TGATGATGAT GATGGTGGTG GTGAAGAATT AGATTCAGAT 
GATGAAGAAA CAGAAGACAA TCTAAATCCT AAACCACAAA GAAGGAAAGG CAGGCGGCGA 
ATATTTTGCC AACTAATGCA CCACCTCACT GAAAACCACA AGATATGGAA GGAAATCATA 
GAAGAAGAAG AAGAAAAATG TAAAGCTGAG GGGAACAAAC TGCAGGTGGA TAATGCCTCC 
TTACCTCAGG CAGATGAGAT TCAGGTTATT GAAGAAGCAG ATGAAGAGGA AGAACAAATG 
TTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507501.1
CDS	3660..6908
Translation	MRKDERERDAPAMRSPPPPPASAASPPESLRNGYVKSCVSPLRQDPPRSFFFHLCRFCNVEPPAASLRAGARLSLGVLAAFVLAALLGARPERWAAAAAGLRTLLSACSLSLSPLFSIACAFFFLTCFLTRAQRGPGRGAGSWWLLALPACCYLGDFAAWQWWSWLRGEPAAAGRLCLVLSCVGLLTLAPRVRLRHGVLVLLFAGLVWWVSFSGLGALPPALRPLLSCLVGGAGCLLALGLDHFFHVRGASPPPRSASTAEEKVPVIRPRRRSSCVSLGESAAGYYGSGKMFRRPSLPCISREQMILWDWDLKQWCKPHYQNSGGGNGVDLSVLNEARNMVSDLLIDPSLPPQVISSLRSISSLMGAFSGSCRPKINSFTPFPGFYPCSEVEDPVEKGDRKLHKGLSGRTSFPTPQLRRSSGASSLLTNEHCSSHGCHLNGPFSSNLFTIPKQRSSSVSLTHHAGLRRAGALPSHSLLNSSSHVPVSAGSLTNRSPIGFPDTTDFLTKPNIILHRSLGSVSSAADFHQYLRNSDSNLCSSCGHQILKYVSTCEPDGTDHPSEKSGEEDSSVFSKEPLNIVETQEEETMKKACRELFLEGDSHLMEEAQQPNIDQEVSLDPMLVEDYDSLIEKMNNWNFQIFELVEKMGEKSGRILSQVMYTLFQDTGLLETFKIPTQEFMNYFRALENGYRDIPYHNRVHATDVLHAVWYLTTRPIPGLPQIHNNHETETKADSDGRLGSGQIAYISSKSCCIPDMSYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLNLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSSENDRLLVCQVCIKLADINGPAKDRDLHLRWTEGIVNEFYEQGDEEATLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWIETEEGDDTESDDDDDDDDGGGEELDSDDEETEDNLNPKPQRRKGRRRIFCQLMHHLTENHKIWKEIIEEEEEKCKAEGNKLQVDNASLPQADEIQVIEEADEEEEQMFE

Target ORF information:

RefSeq Version	XM_006507438.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphodiesterase 3B, cGMP-inhibited (Pde3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507438.3

ORF Insert Sequence:

ATGAGGAAAG ACGAGCGCGA GCGGGACGCG CCAGCCATGA GGTCCCCGCC GCCGCCGCCA 
GCCTCGGCCG CCTCGCCCCC CGAGAGCCTG CGCAACGGCT ACGTGAAGAG CTGCGTGAGC 
CCGCTGCGGC AGGACCCTCC GCGCAGCTTC TTCTTCCACC TCTGCCGCTT CTGCAACGTG 
GAGCCGCCGG CGGCCTCGCT CCGCGCCGGG GCACGCCTCT CGCTCGGCGT CCTGGCCGCC 
TTTGTCCTGG CCGCGCTGCT GGGCGCGAGG CCCGAGCGCT GGGCGGCCGC GGCAGCCGGG 
CTCCGGACGC TGCTGAGCGC CTGCTCGCTC AGCCTCAGCC CGCTCTTCAG CATCGCCTGT 
GCCTTCTTCT TCCTCACCTG TTTCCTGACG CGCGCGCAGC GCGGCCCGGG CCGCGGCGCC 
GGCTCCTGGT GGCTGCTGGC GCTGCCCGCC TGCTGCTACC TGGGCGACTT CGCGGCGTGG 
CAGTGGTGGT CGTGGCTGCG TGGGGAGCCG GCGGCGGCGG GCCGGCTCTG CCTGGTGCTG 
AGCTGCGTGG GGCTGCTGAC GCTCGCGCCC CGCGTGAGGC TGCGGCACGG CGTCCTGGTG 
CTGCTCTTCG CCGGCCTGGT GTGGTGGGTG TCCTTCTCCG GCCTCGGGGC TCTGCCGCCC 
GCGCTCAGGC CTCTGCTGTC GTGCCTGGTC GGGGGCGCGG GATGCCTGCT AGCCCTGGGC 
TTGGACCACT TCTTTCACGT CCGGGGAGCC TCCCCTCCGC CGCGCTCGGC GAGTACCGCG 
GAGGAAAAAG TGCCTGTGAT CAGACCCCGG AGGAGGTCCA GCTGCGTGTC GCTGGGAGAA 
AGCGCAGCCG GTTACTATGG CAGTGGCAAG ATGTTCAGGA GACCGTCGTT GCCTTGTATT 
TCCCGAGAAC AGATGATCCT CTGGGACTGG GACTTGAAGC AGTGGTGTAA ACCTCATTAC 
CAGAATTCTG GAGGTGGAAA TGGAGTTGAT CTTTCAGTGC TAAATGAAGC TCGCAATATG 
GTGTCAGACC TGCTGATTGA CCCAAGCCTT CCCCCACAAG TCATTTCTTC TCTGCGGAGT 
ATCAGTAGCT TGATGGGTGC TTTCTCAGGT TCCTGTAGGC CAAAGATTAA TTCTTTTACA 
CCATTTCCTG GATTTTATCC CTGCTCTGAA GTAGAAGATC CAGTTGAGAA AGGAGATCGA 
AAACTTCACA AGGGATTGAG TGGCAGAACC AGTTTCCCAA CTCCACAGCT GAGGAGGAGC 
TCTGGAGCCT CATCGTTGCT GACTAATGAG CACTGTTCAA GTCATGGATG CCACCTAAAT 
GGGCCTTTTA GTTCAAATCT TTTTACAATT CCAAAGCAGA GGTCATCGTC TGTGTCACTG 
ACGCACCATG CAGGTCTGAG AAGAGCTGGT GCTTTGCCCA GCCACAGTCT TCTGAATTCT 
TCAAGCCATG TACCAGTGTC TGCTGGCTCT CTAACTAATC GATCACCCAT AGGATTTCCT 
GATACCACTG ATTTTCTTAC TAAGCCAAAT ATTATTTTAC ATAGATCACT GGGCAGTGTA 
TCAAGTGCAG CAGATTTCCA TCAGTACCTT AGGAACTCTG ACAGCAATCT GTGTAGCAGC 
TGTGGACACC AAATACTCAA ATATGTTTCA ACATGTGAAC CCGATGGTAC AGACCACCCC 
AGTGAAAAAT CAGGTGAAGA AGACAGCAGT GTTTTCTCAA AAGAACCATT GAACATTGTG 
GAAACCCAAG AAGAAGAGAC CATGAAGAAA GCCTGCAGGG AGTTATTTTT GGAAGGTGAT 
AGTCACCTGA TGGAAGAGGC ACAGCAACCA AATATCGATC AGGAAGTGTC ACTGGATCCA 
ATGTTAGTAG AAGATTATGA TTCATTAATA GAAAAGATGA ACAACTGGAA TTTTCAGATT 
TTTGAGCTTG TAGAAAAGAT GGGAGAAAAA TCAGGAAGGA TTCTCAGTCA GGTTATGTAT 
ACTTTATTTC AAGATACTGG TTTATTGGAA ACATTTAAAA TTCCCACTCA AGAATTTATG 
AATTATTTTC GTGCCTTAGA AAATGGCTAC CGGGACATTC CATATCACAA TCGTGTGCAT 
GCCACAGATG TCCTACATGC TGTTTGGTAT TTGACAACCC GACCAATTCC TGGCTTACCT 
CAGATCCATA ATAACCATGA AACGGAAACC AAAGCAGATT CAGATGGTAG ACTTGGTTCT 
GGACAGATTG CTTACATTTC TTCGAAGAGT TGCTGTATTC CAGATATGAG TTATGGCTGC 
CTATCTTCAA ACATCCCTGC ATTAGAATTG ATGGCTTTGT ATGTGGCAGC TGCCATGCAC 
GATTACGATC ACCCAGGAAG AACAAATGCG TTTCTAGTGG CTACAAATGC ACCTCAGGCA 
GTTTTATACA ATGACAGATC TGTTCTAGAG AATCATCATG CTGCATCAGC TTGGAATCTG 
TATCTTTCTC GCCCAGAGTA CAACTTCCTC CTTAACCTTG ATCACATGGA ATTCAAGCGG 
TTTCGATTTT TAGTTATAGA AGCAATCCTT GCTACAGATC TCAAAAAACA TTTTGATTTC 
CTTGCAGAAT TCAATGCCAA GGCCAATGAT GTAAATAGTA ACGGTATAGA ATGGAGCAGT 
GAAAACGATC GCCTCTTGGT CTGCCAGGTG TGCATCAAAT TAGCAGATAT CAATGGCCCA 
GCAAAAGATC GGGACCTACA TTTGAGATGG ACAGAAGGCA TTGTGAATGA ATTTTATGAG 
CAGGGAGATG AAGAAGCAAC CCTGGGTCTA CCTATTAGTC CCTTCATGGA TCGTTCTTCT 
CCTCAACTAG CAAAACTCCA AGAATCTTTT ATCACCCACA TTGTGGGCCC CCTGTGCAAC 
TCCTATGATG CTGCTGGCTT GCTGCCGGGT CAGTGGATAG AAACAGAAGA GGGTGATGAT 
ACAGAAAGTG ATGATGATGA TGATGATGAT GATGGTGGTG GTGAAGAATT AGATTCAGAT 
GATGAAGAAA CAGAAGACAA TCTAAATCCT AAACCACAAA GAAGGAAAGG CAGGCGGCGA 
ATATTTTGCC AACTAATGCA CCACCTCACT GAAAACCACA AGATATGGAA GGAAATCATA 
GAAGAAGAAG AAGAAAAATG TAAAGCTGAG GGGAACAAAC TGCAGGTGGA TAATGCCTCC 
TTACCTCAGG CAGATGAGAT TCAGGTTATT GAAGAAGCAG ATGAAGAGGA AGAACAAATG 
TTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

White to beige conversion in PDE3B KO adipose tissue through activation of AMPK signaling and mitochondrial function.
Scientific reports740445(2017 Jan)
Chung YW,Ahmad F,Tang Y,Hockman SC,Kee HJ,Berger K,Guirguis E,Choi YH,Schimel DM,Aponte AM,Park S,Degerman E,Manganiello VC

Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels.
The Journal of clinical investigation126(11)4319-4330(2016 Nov)
Ru? L,Ba?ez-Coronel M,Creus-Muncunill J,Giralt A,Alcal?-Vida R,Mentxaka G,Kagerbauer B,Zome?o-Abell?n MT,Aranda Z,Venturi V,P?rez-Navarro E,Estivill X,Mart? E

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Pde3b cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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