Kcnj16 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnj16
Entrez Gene ID	29719
Full Name	potassium voltage-gated channel subfamily J member 16
Synonyms	Kir5.1
General protein information	Preferred Names potassium voltage-gated channel subfamily J member 16 Names inward rectifier potassium channel 16 BIR9 inward rectifier K(+) channel Kir5.1 potassium channel, inwardly rectifying subfamily J member 16 potassium inwardly-rectifying channel, subfamily J, member 16
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	inward rectifying potassium channel; may be involved in renal pH regulation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006247568.2	XP_006247630.1	inward rectifier potassium channel 16 isoform X1
XM_006247569.2	XP_006247631.1	inward rectifier potassium channel 16 isoform X1
XM_006247570.2	XP_006247632.1	inward rectifier potassium channel 16 isoform X1
NM_053314.2	NP_445766.2	inward rectifier potassium channel 16

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KCNJ16	NP_001069762.1
Homo sapiens (human)	KCNJ16	NP_733937.1
	KCNJ16	XP_001083706.1
Canis lupus familiaris (dog)	KCNJ16	XP_005624352.1
Danio rerio (zebrafish)	LOC100001432	XP_001341428.4
Pan troglodytes (chimpanzee)	KCNJ16	XP_523700.2
Rattus norvegicus (Norway rat)	Kcnj16	NP_445766.2
Mus musculus (house mouse)	Kcnj16	NP_001239136.1
Gallus gallus (chicken)	KCNJ16	XP_004946298.1
Xenopus tropicalis (tropical clawed frog)	kcnj16	NP_001120576.1

Gene Ontology
Bibliography

Related articles in PubMed

Essential role of Kir5.1 channels in renal salt handling and blood pressure control.
Palygin O, Levchenko V, Ilatovskaya DV, Pavlov TS, Pochynyuk OM, Jacob HJ, Geurts AM, Hodges MR, Staruschenko A
JCI insight2(18)(2017 Sep)

Astrocytes in the retrotrapezoid nucleus sense H+ by inhibition of a Kir4.1-Kir5.1-like current and may contribute to chemoreception by a purinergic mechanism.
Wenker IC, Kr?neisz O, Nishiyama A, Mulkey DK
Journal of neurophysiology104(6)3042-52(2010 Dec)

Kir5.1 underlies long-lived subconductance levels in heteromeric Kir4.1/Kir5.1 channels from Xenopus tropicalis.
Shang L, Ranson SV, Tucker SJ
Biochemical and biophysical research communications388(3)501-5(2009 Oct)

Novel nucleotide-binding sites in ATP-sensitive potassium channels formed at gating interfaces.
Dong K, Tang LQ, MacGregor GG, Leng Q, Hebert SC
The EMBO journal24(7)1318-29(2005 Apr)

Differential assembly of inwardly rectifying K+ channel subunits, Kir4.1 and Kir5.1, in brain astrocytes.
Hibino H, Fujita A, Iwai K, Yamada M, Kurachi Y
The Journal of biological chemistry279(42)44065-73(2004 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Heteromeric Kir4.1-Kir5.1 channels confer pH sensitivity to retrotrapezoid nucleus astrocytes.
Title: Astrocytes in the retrotrapezoid nucleus sense H+ by inhibition of a Kir4.1-Kir5.1-like current and may contribute to chemoreception by a purinergic mechanism.

data suggest the signals on the COOH terminus of Kir4.1 potassium channel determine the intracellular localization of Kir5.1 potassium channel/Kir4.1 heteromer in distal tubules
Title: PDZ binding motif-dependent localization of K+ channel on the basolateral side in distal tubules.

Specific immunoreactivity for Kir5.1 was detected in fibrocytes of the cochlear spiral ligament and spiral limbus, all of which are directly involved in K+ circulation.
Title: Expression of an inwardly rectifying K+ channel, Kir5.1, in specific types of fibrocytes in the cochlear lateral wall suggests its functional importance in the establishment of endocochlear potential.

examined the heteromeric assembly of Kir5.1 with other Kir subunits and have shown that this subunit exhibits a highly selective interaction with members of the Kir4.0 subfamily and does not physically associate with other Kir subunits
Title: Identification of domains that control the heteromeric assembly of Kir5.1/Kir4.0 potassium channels.

The following Kcnj16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnj16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa33844	NM_053314.2 Latest version!	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 16 (Kcnj16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa33844	XM_006247568.2 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily J member 16 (Kcnj16), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa33844	XM_006247570.2 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily J member 16 (Kcnj16), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa33844	XM_006247569.2 Latest version!	Rattus norvegicus potassium voltage-gated channel subfamily J member 16 (Kcnj16), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnj16 ( NM_053314.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445766.2 Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 16 (Kcnj16), mRNA.

Price & Availability↑

CloneID	ORa33844
Clone ID Related Accession (Same CDS sequence)	XM_006247568.2 , XM_006247569.2 , XM_006247570.2 , NM_053314.2
Accession Version	NM_053314.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	inward rectifier potassium channel 16
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078776.1. On Aug 29, 2004 this sequence version replaced NM_053314.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078776.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCTATT ACGGAAGTAG CTACCGGATT GTCAACGTGG ACTCCAAATA TCCAGGCTAT 
CCTCCAGAGC ATGCCATAGC CGAGAAGAGA AGAGCGAGGA GGCGCCTGCT CCACAAAGAT 
GGCAGCTGTA ACGTGTACTT TAAACACATT TTTGGAGAAT GGGGGAGCTA CATGGTTGAT 
ATATTCACCA CTCTTGTGGA TACCAAGTGG CGCCATATGT TTGTAATATT TTCTTTGTCT 
TACATTCTCT CCTGGTTGAT ATTCGGCTCC ATATTTTGGC TCATAGCCCT TCATCACGGA 
GACCTATTAA GCGATCCAGA CATCACCCCG TGCGTGGACA ACGTGCATTC ATTTACGGCG 
GCGTTTTTAT TCTCCCTTGA GACCCAAACC ACCATCGGGT ACGGTTACCG TTGTGTCACG 
GAAGAATGCT CTGTGGCCGT GCTGACGGTC ATCCTTCAGT CCATCCTGAG CTGCATCATA 
AACACCTTCA TCATTGGGGC AGCCTTGGCA AAGATGGCGA CCGCCCGGAA GCGAGCCCAG 
ACCATCCGCT TCAGCTATTT CGCACTCATC GGCATGAGAG ACGGGAAGCT TTGCCTCATG 
TGGCGCATAG GTGACTTCCG ACCCAACCAT GTGGTGGAGG GCACGGTGAG AGCCCAACTT 
CTGCGCTATT CAGAAGACAG CGAAGGGCGG ATGACGATGG CGTTTAAAGA CCTCAAACTC 
GTCAATGACC AGATAATCCT GGTAACGCCA GTGACCATTG TCCATGAAAT TGACCACGAG 
AGTCCTCTGT ATGCCCTTGA CCGCAAGGCA GTGGCCAAAG ATAATTTCGA GATTCTGGTG 
ACATTTATTT ATACTGGTGA CTCTACTGGG ACATCCCACC AGTCCAGAAG TTCCTACGTC 
CCCAGAGAAA TTCTCTGGGG ACACAGGTTT CATGATGTAT TGGAAGTGAA GAGAAAGTAC 
TACAAGGTAA ACTGCTTGCA GTTTGAGGGA AGCGTGGAAG TCTACGCCCC CTTTTGCAGT 
GCCAAACAAC TGGACTGGAA GGACCAACAG CTCAACAACT TGGAGAAAAC GTCCCCTGCC 
CGAGGATCCT GCACCTCGGA CACCAACACC AGGAGGAGAT CCTTCAGCGC AGTTGCCATG 
GTGAGCAGCT GTGAGAACCC AGAGGAGACC AGCCTGTCCC CACAAGATGA GTGTAAGGAG 
GTCCCCTATC AGAAAGCCCT CCTGACTTTA AACAGGATCT CCATGGAATC CCAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445766.2
CDS	44..1303
Translation	MSYYGSSYRIVNVDSKYPGYPPEHAIAEKRRARRRLLHKDGSCNVYFKHIFGEWGSYMVDIFTTLVDTKWRHMFVIFSLSYILSWLIFGSIFWLIALHHGDLLSDPDITPCVDNVHSFTAAFLFSLETQTTIGYGYRCVTEECSVAVLTVILQSILSCIINTFIIGAALAKMATARKRAQTIRFSYFALIGMRDGKLCLMWRIGDFRPNHVVEGTVRAQLLRYSEDSEGRMTMAFKDLKLVNDQIILVTPVTIVHEIDHESPLYALDRKAVAKDNFEILVTFIYTGDSTGTSHQSRSSYVPREILWGHRFHDVLEVKRKYYKVNCLQFEGSVEVYAPFCSAKQLDWKDQQLNNLEKTSPARGSCTSDTNTRRRSFSAVAMVSSCENPEETSLSPQDECKEVPYQKALLTLNRISMESQM

Target ORF information:

RefSeq Version	NM_053314.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium inwardly-rectifying channel, subfamily J, member 16 (Kcnj16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053314.2

ORF Insert Sequence:

ATGAGCTATT ACGGAAGTAG CTACCGGATT GTCAACGTGG ACTCCAAATA TCCAGGCTAT 
CCTCCAGAGC ATGCCATAGC CGAGAAGAGA AGAGCGAGGA GGCGCCTGCT CCACAAAGAT 
GGCAGCTGTA ACGTGTACTT TAAACACATT TTTGGAGAAT GGGGGAGCTA CATGGTTGAT 
ATATTCACCA CTCTTGTGGA TACCAAGTGG CGCCATATGT TTGTAATATT TTCTTTGTCT 
TACATTCTCT CCTGGTTGAT ATTCGGCTCC ATATTTTGGC TCATAGCCCT TCATCACGGA 
GACCTATTAA GCGATCCAGA CATCACCCCG TGCGTGGACA ACGTGCATTC ATTTACGGCG 
GCGTTTTTAT TCTCCCTTGA GACCCAAACC ACCATCGGGT ACGGTTACCG TTGTGTCACG 
GAAGAATGCT CTGTGGCCGT GCTGACGGTC ATCCTTCAGT CCATCCTGAG CTGCATCATA 
AACACCTTCA TCATTGGGGC AGCCTTGGCA AAGATGGCGA CCGCCCGGAA GCGAGCCCAG 
ACCATCCGCT TCAGCTATTT CGCACTCATC GGCATGAGAG ACGGGAAGCT TTGCCTCATG 
TGGCGCATAG GTGACTTCCG ACCCAACCAT GTGGTGGAGG GCACGGTGAG AGCCCAACTT 
CTGCGCTATT CAGAAGACAG CGAAGGGCGG ATGACGATGG CGTTTAAAGA CCTCAAACTC 
GTCAATGACC AGATAATCCT GGTAACGCCA GTGACCATTG TCCATGAAAT TGACCACGAG 
AGTCCTCTGT ATGCCCTTGA CCGCAAGGCA GTGGCCAAAG ATAATTTCGA GATTCTGGTG 
ACATTTATTT ATACTGGTGA CTCTACTGGG ACATCCCACC AGTCCAGAAG TTCCTACGTC 
CCCAGAGAAA TTCTCTGGGG ACACAGGTTT CATGATGTAT TGGAAGTGAA GAGAAAGTAC 
TACAAGGTAA ACTGCTTGCA GTTTGAGGGA AGCGTGGAAG TCTACGCCCC CTTTTGCAGT 
GCCAAACAAC TGGACTGGAA GGACCAACAG CTCAACAACT TGGAGAAAAC GTCCCCTGCC 
CGAGGATCCT GCACCTCGGA CACCAACACC AGGAGGAGAT CCTTCAGCGC AGTTGCCATG 
GTGAGCAGCT GTGAGAACCC AGAGGAGACC AGCCTGTCCC CACAAGATGA GTGTAAGGAG 
GTCCCCTATC AGAAAGCCCT CCTGACTTTA AACAGGATCT CCATGGAATC CCAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj16 ( XM_006247568.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247630.1 Rattus norvegicus potassium voltage-gated channel subfamily J member 16 (Kcnj16), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33844
Clone ID Related Accession (Same CDS sequence)	XM_006247568.2 , XM_006247569.2 , XM_006247570.2 , NM_053314.2
Accession Version	XM_006247568.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inward rectifier potassium channel 16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006247568.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTATT ACGGAAGTAG CTACCGGATT GTCAACGTGG ACTCCAAATA TCCAGGCTAT 
CCTCCAGAGC ATGCCATAGC CGAGAAGAGA AGAGCGAGGA GGCGCCTGCT CCACAAAGAT 
GGCAGCTGTA ACGTGTACTT TAAACACATT TTTGGAGAAT GGGGGAGCTA CATGGTTGAT 
ATATTCACCA CTCTTGTGGA TACCAAGTGG CGCCATATGT TTGTAATATT TTCTTTGTCT 
TACATTCTCT CCTGGTTGAT ATTCGGCTCC ATATTTTGGC TCATAGCCCT TCATCACGGA 
GACCTATTAA GCGATCCAGA CATCACCCCG TGCGTGGACA ACGTGCATTC ATTTACGGCG 
GCGTTTTTAT TCTCCCTTGA GACCCAAACC ACCATCGGGT ACGGTTACCG TTGTGTCACG 
GAAGAATGCT CTGTGGCCGT GCTGACGGTC ATCCTTCAGT CCATCCTGAG CTGCATCATA 
AACACCTTCA TCATTGGGGC AGCCTTGGCA AAGATGGCGA CCGCCCGGAA GCGAGCCCAG 
ACCATCCGCT TCAGCTATTT CGCACTCATC GGCATGAGAG ACGGGAAGCT TTGCCTCATG 
TGGCGCATAG GTGACTTCCG ACCCAACCAT GTGGTGGAGG GCACGGTGAG AGCCCAACTT 
CTGCGCTATT CAGAAGACAG CGAAGGGCGG ATGACGATGG CGTTTAAAGA CCTCAAACTC 
GTCAATGACC AGATAATCCT GGTAACGCCA GTGACCATTG TCCATGAAAT TGACCACGAG 
AGTCCTCTGT ATGCCCTTGA CCGCAAGGCA GTGGCCAAAG ATAATTTCGA GATTCTGGTG 
ACATTTATTT ATACTGGTGA CTCTACTGGG ACATCCCACC AGTCCAGAAG TTCCTACGTC 
CCCAGAGAAA TTCTCTGGGG ACACAGGTTT CATGATGTAT TGGAAGTGAA GAGAAAGTAC 
TACAAGGTAA ACTGCTTGCA GTTTGAGGGA AGCGTGGAAG TCTACGCCCC CTTTTGCAGT 
GCCAAACAAC TGGACTGGAA GGACCAACAG CTCAACAACT TGGAGAAAAC GTCCCCTGCC 
CGAGGATCCT GCACCTCGGA CACCAACACC AGGAGGAGAT CCTTCAGCGC AGTTGCCATG 
GTGAGCAGCT GTGAGAACCC AGAGGAGACC AGCCTGTCCC CACAAGATGA GTGTAAGGAG 
GTCCCCTATC AGAAAGCCCT CCTGACTTTA AACAGGATCT CCATGGAATC CCAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247630.1
CDS	197..1456
Translation	MSYYGSSYRIVNVDSKYPGYPPEHAIAEKRRARRRLLHKDGSCNVYFKHIFGEWGSYMVDIFTTLVDTKWRHMFVIFSLSYILSWLIFGSIFWLIALHHGDLLSDPDITPCVDNVHSFTAAFLFSLETQTTIGYGYRCVTEECSVAVLTVILQSILSCIINTFIIGAALAKMATARKRAQTIRFSYFALIGMRDGKLCLMWRIGDFRPNHVVEGTVRAQLLRYSEDSEGRMTMAFKDLKLVNDQIILVTPVTIVHEIDHESPLYALDRKAVAKDNFEILVTFIYTGDSTGTSHQSRSSYVPREILWGHRFHDVLEVKRKYYKVNCLQFEGSVEVYAPFCSAKQLDWKDQQLNNLEKTSPARGSCTSDTNTRRRSFSAVAMVSSCENPEETSLSPQDECKEVPYQKALLTLNRISMESQM

Target ORF information:

RefSeq Version	XM_006247568.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily J member 16 (Kcnj16), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247568.2

ORF Insert Sequence:

ATGAGCTATT ACGGAAGTAG CTACCGGATT GTCAACGTGG ACTCCAAATA TCCAGGCTAT 
CCTCCAGAGC ATGCCATAGC CGAGAAGAGA AGAGCGAGGA GGCGCCTGCT CCACAAAGAT 
GGCAGCTGTA ACGTGTACTT TAAACACATT TTTGGAGAAT GGGGGAGCTA CATGGTTGAT 
ATATTCACCA CTCTTGTGGA TACCAAGTGG CGCCATATGT TTGTAATATT TTCTTTGTCT 
TACATTCTCT CCTGGTTGAT ATTCGGCTCC ATATTTTGGC TCATAGCCCT TCATCACGGA 
GACCTATTAA GCGATCCAGA CATCACCCCG TGCGTGGACA ACGTGCATTC ATTTACGGCG 
GCGTTTTTAT TCTCCCTTGA GACCCAAACC ACCATCGGGT ACGGTTACCG TTGTGTCACG 
GAAGAATGCT CTGTGGCCGT GCTGACGGTC ATCCTTCAGT CCATCCTGAG CTGCATCATA 
AACACCTTCA TCATTGGGGC AGCCTTGGCA AAGATGGCGA CCGCCCGGAA GCGAGCCCAG 
ACCATCCGCT TCAGCTATTT CGCACTCATC GGCATGAGAG ACGGGAAGCT TTGCCTCATG 
TGGCGCATAG GTGACTTCCG ACCCAACCAT GTGGTGGAGG GCACGGTGAG AGCCCAACTT 
CTGCGCTATT CAGAAGACAG CGAAGGGCGG ATGACGATGG CGTTTAAAGA CCTCAAACTC 
GTCAATGACC AGATAATCCT GGTAACGCCA GTGACCATTG TCCATGAAAT TGACCACGAG 
AGTCCTCTGT ATGCCCTTGA CCGCAAGGCA GTGGCCAAAG ATAATTTCGA GATTCTGGTG 
ACATTTATTT ATACTGGTGA CTCTACTGGG ACATCCCACC AGTCCAGAAG TTCCTACGTC 
CCCAGAGAAA TTCTCTGGGG ACACAGGTTT CATGATGTAT TGGAAGTGAA GAGAAAGTAC 
TACAAGGTAA ACTGCTTGCA GTTTGAGGGA AGCGTGGAAG TCTACGCCCC CTTTTGCAGT 
GCCAAACAAC TGGACTGGAA GGACCAACAG CTCAACAACT TGGAGAAAAC GTCCCCTGCC 
CGAGGATCCT GCACCTCGGA CACCAACACC AGGAGGAGAT CCTTCAGCGC AGTTGCCATG 
GTGAGCAGCT GTGAGAACCC AGAGGAGACC AGCCTGTCCC CACAAGATGA GTGTAAGGAG 
GTCCCCTATC AGAAAGCCCT CCTGACTTTA AACAGGATCT CCATGGAATC CCAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj16 ( XM_006247569.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247631.1 Rattus norvegicus potassium voltage-gated channel subfamily J member 16 (Kcnj16), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa33844
Clone ID Related Accession (Same CDS sequence)	XM_006247568.2 , XM_006247569.2 , XM_006247570.2 , NM_053314.2
Accession Version	XM_006247569.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inward rectifier potassium channel 16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006247569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTATT ACGGAAGTAG CTACCGGATT GTCAACGTGG ACTCCAAATA TCCAGGCTAT 
CCTCCAGAGC ATGCCATAGC CGAGAAGAGA AGAGCGAGGA GGCGCCTGCT CCACAAAGAT 
GGCAGCTGTA ACGTGTACTT TAAACACATT TTTGGAGAAT GGGGGAGCTA CATGGTTGAT 
ATATTCACCA CTCTTGTGGA TACCAAGTGG CGCCATATGT TTGTAATATT TTCTTTGTCT 
TACATTCTCT CCTGGTTGAT ATTCGGCTCC ATATTTTGGC TCATAGCCCT TCATCACGGA 
GACCTATTAA GCGATCCAGA CATCACCCCG TGCGTGGACA ACGTGCATTC ATTTACGGCG 
GCGTTTTTAT TCTCCCTTGA GACCCAAACC ACCATCGGGT ACGGTTACCG TTGTGTCACG 
GAAGAATGCT CTGTGGCCGT GCTGACGGTC ATCCTTCAGT CCATCCTGAG CTGCATCATA 
AACACCTTCA TCATTGGGGC AGCCTTGGCA AAGATGGCGA CCGCCCGGAA GCGAGCCCAG 
ACCATCCGCT TCAGCTATTT CGCACTCATC GGCATGAGAG ACGGGAAGCT TTGCCTCATG 
TGGCGCATAG GTGACTTCCG ACCCAACCAT GTGGTGGAGG GCACGGTGAG AGCCCAACTT 
CTGCGCTATT CAGAAGACAG CGAAGGGCGG ATGACGATGG CGTTTAAAGA CCTCAAACTC 
GTCAATGACC AGATAATCCT GGTAACGCCA GTGACCATTG TCCATGAAAT TGACCACGAG 
AGTCCTCTGT ATGCCCTTGA CCGCAAGGCA GTGGCCAAAG ATAATTTCGA GATTCTGGTG 
ACATTTATTT ATACTGGTGA CTCTACTGGG ACATCCCACC AGTCCAGAAG TTCCTACGTC 
CCCAGAGAAA TTCTCTGGGG ACACAGGTTT CATGATGTAT TGGAAGTGAA GAGAAAGTAC 
TACAAGGTAA ACTGCTTGCA GTTTGAGGGA AGCGTGGAAG TCTACGCCCC CTTTTGCAGT 
GCCAAACAAC TGGACTGGAA GGACCAACAG CTCAACAACT TGGAGAAAAC GTCCCCTGCC 
CGAGGATCCT GCACCTCGGA CACCAACACC AGGAGGAGAT CCTTCAGCGC AGTTGCCATG 
GTGAGCAGCT GTGAGAACCC AGAGGAGACC AGCCTGTCCC CACAAGATGA GTGTAAGGAG 
GTCCCCTATC AGAAAGCCCT CCTGACTTTA AACAGGATCT CCATGGAATC CCAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247631.1
CDS	284..1543
Translation	MSYYGSSYRIVNVDSKYPGYPPEHAIAEKRRARRRLLHKDGSCNVYFKHIFGEWGSYMVDIFTTLVDTKWRHMFVIFSLSYILSWLIFGSIFWLIALHHGDLLSDPDITPCVDNVHSFTAAFLFSLETQTTIGYGYRCVTEECSVAVLTVILQSILSCIINTFIIGAALAKMATARKRAQTIRFSYFALIGMRDGKLCLMWRIGDFRPNHVVEGTVRAQLLRYSEDSEGRMTMAFKDLKLVNDQIILVTPVTIVHEIDHESPLYALDRKAVAKDNFEILVTFIYTGDSTGTSHQSRSSYVPREILWGHRFHDVLEVKRKYYKVNCLQFEGSVEVYAPFCSAKQLDWKDQQLNNLEKTSPARGSCTSDTNTRRRSFSAVAMVSSCENPEETSLSPQDECKEVPYQKALLTLNRISMESQM

Target ORF information:

RefSeq Version	XM_006247569.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily J member 16 (Kcnj16), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247569.2

ORF Insert Sequence:

ATGAGCTATT ACGGAAGTAG CTACCGGATT GTCAACGTGG ACTCCAAATA TCCAGGCTAT 
CCTCCAGAGC ATGCCATAGC CGAGAAGAGA AGAGCGAGGA GGCGCCTGCT CCACAAAGAT 
GGCAGCTGTA ACGTGTACTT TAAACACATT TTTGGAGAAT GGGGGAGCTA CATGGTTGAT 
ATATTCACCA CTCTTGTGGA TACCAAGTGG CGCCATATGT TTGTAATATT TTCTTTGTCT 
TACATTCTCT CCTGGTTGAT ATTCGGCTCC ATATTTTGGC TCATAGCCCT TCATCACGGA 
GACCTATTAA GCGATCCAGA CATCACCCCG TGCGTGGACA ACGTGCATTC ATTTACGGCG 
GCGTTTTTAT TCTCCCTTGA GACCCAAACC ACCATCGGGT ACGGTTACCG TTGTGTCACG 
GAAGAATGCT CTGTGGCCGT GCTGACGGTC ATCCTTCAGT CCATCCTGAG CTGCATCATA 
AACACCTTCA TCATTGGGGC AGCCTTGGCA AAGATGGCGA CCGCCCGGAA GCGAGCCCAG 
ACCATCCGCT TCAGCTATTT CGCACTCATC GGCATGAGAG ACGGGAAGCT TTGCCTCATG 
TGGCGCATAG GTGACTTCCG ACCCAACCAT GTGGTGGAGG GCACGGTGAG AGCCCAACTT 
CTGCGCTATT CAGAAGACAG CGAAGGGCGG ATGACGATGG CGTTTAAAGA CCTCAAACTC 
GTCAATGACC AGATAATCCT GGTAACGCCA GTGACCATTG TCCATGAAAT TGACCACGAG 
AGTCCTCTGT ATGCCCTTGA CCGCAAGGCA GTGGCCAAAG ATAATTTCGA GATTCTGGTG 
ACATTTATTT ATACTGGTGA CTCTACTGGG ACATCCCACC AGTCCAGAAG TTCCTACGTC 
CCCAGAGAAA TTCTCTGGGG ACACAGGTTT CATGATGTAT TGGAAGTGAA GAGAAAGTAC 
TACAAGGTAA ACTGCTTGCA GTTTGAGGGA AGCGTGGAAG TCTACGCCCC CTTTTGCAGT 
GCCAAACAAC TGGACTGGAA GGACCAACAG CTCAACAACT TGGAGAAAAC GTCCCCTGCC 
CGAGGATCCT GCACCTCGGA CACCAACACC AGGAGGAGAT CCTTCAGCGC AGTTGCCATG 
GTGAGCAGCT GTGAGAACCC AGAGGAGACC AGCCTGTCCC CACAAGATGA GTGTAAGGAG 
GTCCCCTATC AGAAAGCCCT CCTGACTTTA AACAGGATCT CCATGGAATC CCAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnj16 ( XM_006247570.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247632.1 Rattus norvegicus potassium voltage-gated channel subfamily J member 16 (Kcnj16), transcript variant X3, mRNA.

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CloneID	ORa33844
Clone ID Related Accession (Same CDS sequence)	XM_006247568.2 , XM_006247569.2 , XM_006247570.2 , NM_053314.2
Accession Version	XM_006247570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inward rectifier potassium channel 16 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006247570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTATT ACGGAAGTAG CTACCGGATT GTCAACGTGG ACTCCAAATA TCCAGGCTAT 
CCTCCAGAGC ATGCCATAGC CGAGAAGAGA AGAGCGAGGA GGCGCCTGCT CCACAAAGAT 
GGCAGCTGTA ACGTGTACTT TAAACACATT TTTGGAGAAT GGGGGAGCTA CATGGTTGAT 
ATATTCACCA CTCTTGTGGA TACCAAGTGG CGCCATATGT TTGTAATATT TTCTTTGTCT 
TACATTCTCT CCTGGTTGAT ATTCGGCTCC ATATTTTGGC TCATAGCCCT TCATCACGGA 
GACCTATTAA GCGATCCAGA CATCACCCCG TGCGTGGACA ACGTGCATTC ATTTACGGCG 
GCGTTTTTAT TCTCCCTTGA GACCCAAACC ACCATCGGGT ACGGTTACCG TTGTGTCACG 
GAAGAATGCT CTGTGGCCGT GCTGACGGTC ATCCTTCAGT CCATCCTGAG CTGCATCATA 
AACACCTTCA TCATTGGGGC AGCCTTGGCA AAGATGGCGA CCGCCCGGAA GCGAGCCCAG 
ACCATCCGCT TCAGCTATTT CGCACTCATC GGCATGAGAG ACGGGAAGCT TTGCCTCATG 
TGGCGCATAG GTGACTTCCG ACCCAACCAT GTGGTGGAGG GCACGGTGAG AGCCCAACTT 
CTGCGCTATT CAGAAGACAG CGAAGGGCGG ATGACGATGG CGTTTAAAGA CCTCAAACTC 
GTCAATGACC AGATAATCCT GGTAACGCCA GTGACCATTG TCCATGAAAT TGACCACGAG 
AGTCCTCTGT ATGCCCTTGA CCGCAAGGCA GTGGCCAAAG ATAATTTCGA GATTCTGGTG 
ACATTTATTT ATACTGGTGA CTCTACTGGG ACATCCCACC AGTCCAGAAG TTCCTACGTC 
CCCAGAGAAA TTCTCTGGGG ACACAGGTTT CATGATGTAT TGGAAGTGAA GAGAAAGTAC 
TACAAGGTAA ACTGCTTGCA GTTTGAGGGA AGCGTGGAAG TCTACGCCCC CTTTTGCAGT 
GCCAAACAAC TGGACTGGAA GGACCAACAG CTCAACAACT TGGAGAAAAC GTCCCCTGCC 
CGAGGATCCT GCACCTCGGA CACCAACACC AGGAGGAGAT CCTTCAGCGC AGTTGCCATG 
GTGAGCAGCT GTGAGAACCC AGAGGAGACC AGCCTGTCCC CACAAGATGA GTGTAAGGAG 
GTCCCCTATC AGAAAGCCCT CCTGACTTTA AACAGGATCT CCATGGAATC CCAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247632.1
CDS	862..2121
Translation	MSYYGSSYRIVNVDSKYPGYPPEHAIAEKRRARRRLLHKDGSCNVYFKHIFGEWGSYMVDIFTTLVDTKWRHMFVIFSLSYILSWLIFGSIFWLIALHHGDLLSDPDITPCVDNVHSFTAAFLFSLETQTTIGYGYRCVTEECSVAVLTVILQSILSCIINTFIIGAALAKMATARKRAQTIRFSYFALIGMRDGKLCLMWRIGDFRPNHVVEGTVRAQLLRYSEDSEGRMTMAFKDLKLVNDQIILVTPVTIVHEIDHESPLYALDRKAVAKDNFEILVTFIYTGDSTGTSHQSRSSYVPREILWGHRFHDVLEVKRKYYKVNCLQFEGSVEVYAPFCSAKQLDWKDQQLNNLEKTSPARGSCTSDTNTRRRSFSAVAMVSSCENPEETSLSPQDECKEVPYQKALLTLNRISMESQM

Target ORF information:

RefSeq Version	XM_006247570.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus potassium voltage-gated channel subfamily J member 16 (Kcnj16), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247570.2

ORF Insert Sequence:

ATGAGCTATT ACGGAAGTAG CTACCGGATT GTCAACGTGG ACTCCAAATA TCCAGGCTAT 
CCTCCAGAGC ATGCCATAGC CGAGAAGAGA AGAGCGAGGA GGCGCCTGCT CCACAAAGAT 
GGCAGCTGTA ACGTGTACTT TAAACACATT TTTGGAGAAT GGGGGAGCTA CATGGTTGAT 
ATATTCACCA CTCTTGTGGA TACCAAGTGG CGCCATATGT TTGTAATATT TTCTTTGTCT 
TACATTCTCT CCTGGTTGAT ATTCGGCTCC ATATTTTGGC TCATAGCCCT TCATCACGGA 
GACCTATTAA GCGATCCAGA CATCACCCCG TGCGTGGACA ACGTGCATTC ATTTACGGCG 
GCGTTTTTAT TCTCCCTTGA GACCCAAACC ACCATCGGGT ACGGTTACCG TTGTGTCACG 
GAAGAATGCT CTGTGGCCGT GCTGACGGTC ATCCTTCAGT CCATCCTGAG CTGCATCATA 
AACACCTTCA TCATTGGGGC AGCCTTGGCA AAGATGGCGA CCGCCCGGAA GCGAGCCCAG 
ACCATCCGCT TCAGCTATTT CGCACTCATC GGCATGAGAG ACGGGAAGCT TTGCCTCATG 
TGGCGCATAG GTGACTTCCG ACCCAACCAT GTGGTGGAGG GCACGGTGAG AGCCCAACTT 
CTGCGCTATT CAGAAGACAG CGAAGGGCGG ATGACGATGG CGTTTAAAGA CCTCAAACTC 
GTCAATGACC AGATAATCCT GGTAACGCCA GTGACCATTG TCCATGAAAT TGACCACGAG 
AGTCCTCTGT ATGCCCTTGA CCGCAAGGCA GTGGCCAAAG ATAATTTCGA GATTCTGGTG 
ACATTTATTT ATACTGGTGA CTCTACTGGG ACATCCCACC AGTCCAGAAG TTCCTACGTC 
CCCAGAGAAA TTCTCTGGGG ACACAGGTTT CATGATGTAT TGGAAGTGAA GAGAAAGTAC 
TACAAGGTAA ACTGCTTGCA GTTTGAGGGA AGCGTGGAAG TCTACGCCCC CTTTTGCAGT 
GCCAAACAAC TGGACTGGAA GGACCAACAG CTCAACAACT TGGAGAAAAC GTCCCCTGCC 
CGAGGATCCT GCACCTCGGA CACCAACACC AGGAGGAGAT CCTTCAGCGC AGTTGCCATG 
GTGAGCAGCT GTGAGAACCC AGAGGAGACC AGCCTGTCCC CACAAGATGA GTGTAAGGAG 
GTCCCCTATC AGAAAGCCCT CCTGACTTTA AACAGGATCT CCATGGAATC CCAGATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Essential role of Kir5.1 channels in renal salt handling and blood pressure control.
JCI insight2(18)(2017 Sep)
Palygin O,Levchenko V,Ilatovskaya DV,Pavlov TS,Pochynyuk OM,Jacob HJ,Geurts AM,Hodges MR,Staruschenko A

Astrocytes in the retrotrapezoid nucleus sense H+ by inhibition of a Kir4.1-Kir5.1-like current and may contribute to chemoreception by a purinergic mechanism.
Journal of neurophysiology104(6)3042-52(2010 Dec)
Wenker IC,Kr?neisz O,Nishiyama A,Mulkey DK

Kir5.1 underlies long-lived subconductance levels in heteromeric Kir4.1/Kir5.1 channels from Xenopus tropicalis.
Biochemical and biophysical research communications388(3)501-5(2009 Oct)
Shang L,Ranson SV,Tucker SJ

Novel nucleotide-binding sites in ATP-sensitive potassium channels formed at gating interfaces.
The EMBO journal24(7)1318-29(2005 Apr)
Dong K,Tang LQ,MacGregor GG,Leng Q,Hebert SC

Differential assembly of inwardly rectifying K+ channel subunits, Kir4.1 and Kir5.1, in brain astrocytes.
The Journal of biological chemistry279(42)44065-73(2004 Oct)
Hibino H,Fujita A,Iwai K,Yamada M,Kurachi Y