Nfu1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Nfu1
Entrez Gene ID	297416
Full Name	NFU1 iron-sulfur cluster scaffold
Synonyms	Hirip5
General protein information	Preferred Names NFU1 iron-sulfur cluster scaffold Names NFU1 iron-sulfur cluster scaffold homolog, mitochondrial histone cell cycle regulation defective interacting protein 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_008763063.2	XP_008761285.1	NFU1 iron-sulfur cluster scaffold homolog, mitochondrial isoform X1
NM_001106606.2	NP_001100076.2	NFU1 iron-sulfur cluster scaffold homolog, mitochondrial precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nfu1	NP_001100076.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000598	XP_310479.5
Eremothecium gossypii	AGOS_ABR045W	NP_982991.1
Arabidopsis thaliana (thale cress)	NFU5	NP_175550.2
Xenopus tropicalis (tropical clawed frog)	nfu1	NP_001072356.1
Homo sapiens (human)	NFU1	NP_001002755.1
Danio rerio (zebrafish)	nfu1	NP_001116180.1
Neurospora crassa	NCU01779	XP_957202.1
Pan troglodytes (chimpanzee)	NFU1	XP_525775.1
Bos taurus (cattle)	NFU1	NP_001040031.1
Gallus gallus (chicken)	NFU1	NP_001006305.2
Drosophila melanogaster (fruit fly)	CG32857	NP_728443.1
Kluyveromyces lactis	KLLA0F25476g	XP_456215.1
Arabidopsis thaliana (thale cress)	NFU4	NP_566673.1
Canis lupus familiaris (dog)	NFU1	XP_855433.2
Mus musculus (house mouse)	Nfu1	NP_001164062.1
Drosophila melanogaster (fruit fly)	CG32500	NP_728447.2
Saccharomyces cerevisiae (baker's yeast)	NFU1	NP_012884.3
Magnaporthe oryzae (rice blast fungus)	MGG_06993	XP_003709745.1
Drosophila melanogaster (fruit fly)	CG33502	NP_996529.1
Caenorhabditis elegans (roundworm)	lpd-8	NP_501917.1
Schizosaccharomyces pombe (fission yeast)	SPBC1709.19c	NP_595452.2
Oryza sativa (rice)	Os05g0155300	NP_001054690.1

Gene Ontology
Bibliography

Related articles in PubMed

Understanding the molecular basis for multiple mitochondrial dysfunctions syndrome 1 (MMDS1): impact of a disease-causing Gly189Arg substitution on NFU1.
Wesley NA, Wachnowsky C, Fidai I, Cowan JA
The FEBS journal284(22)3838-3848(2017 Nov)

Iron-sulfur cluster biogenesis in mammalian cells: New insights into the molecular mechanisms of cluster delivery.
Maio N, Rouault TA
Biochimica et biophysica acta1853(6)1493-512(2015 Jun)

The Lafora disease gene product laforin interacts with HIRIP5, a phylogenetically conserved protein containing a NifU-like domain.
Ganesh S, Tsurutani N, Suzuki T, Ueda K, Agarwala KL, Osada H, Delgado-Escueta AV, Yamakawa K
Human molecular genetics12(18)2359-68(2003 Sep)

Subcellular compartmentalization of human Nfu, an iron-sulfur cluster scaffold protein, and its ability to assemble a [4Fe-4S] cluster.
Tong WH, Jameson GN, Huynh BH, Rouault TA
Proceedings of the National Academy of Sciences of the United States of America100(17)9762-7(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nfu1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfu1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01771	NM_001106606.2 Latest version!	Rattus norvegicus NFU1 iron-sulfur cluster scaffold (Nfu1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa45451	XM_008763063.2 Latest version!	Rattus norvegicus NFU1 iron-sulfur cluster scaffold (Nfu1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nfu1 ( NM_001106606.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100076.2 Rattus norvegicus NFU1 iron-sulfur cluster scaffold (Nfu1), mRNA.

Price & Availability↑

CloneID	ORa01771
Clone ID Related Accession (Same CDS sequence)	NM_001106606.2
Accession Version	NM_001106606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Rattus norvegicus(Norway rat)
Product	NFU1 iron-sulfur cluster scaffold homolog, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM058785.1, FM064033.1 and FM092780.1. On Dec 25, 2009 this sequence version replaced NM_001106606.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCGG CCGAGCGGGC GTGGGGAGCA GCTGTCGGTG TAGCCCGGCT GTGCAGGCGG 
CTCTGTCATG TGGCGACTCC ACACGCCTTT AAGAAACAGC CTCTGCACCA ATATGTACAG 
AGACCACTCT TCCCTCTGCG CACGCCCTTG TATAACACAG TACGATTCAT GTTTATTCAA 
ACACAAGACA CCCCAAATCC CAACAGCTTA AAGTTTATAC CAGGAAAGCC GGTTCTTGAG 
ACGAGGACCA TGGATTTCCC CACACCAGCT GCGGCTTTCC GTTCCCCTCT GGCCAGGCAG 
TTATTCAGGA TTGAAGGTGT GAAAAGTGTC TTCTTTGGGC CAGATTTCAT TACAGTCACA 
AAGGAGAATG AAGAATTAGA CTGGAATTTA CTGAAACCAG ACATTTATGC AACAATTATG 
GACTTCTTTG CATCTGGCTT ACCTCTAGTG ACTGAGGAGA CGCCTCCAGG AGAAGCAGGT 
TCTGAGGAAG ATGACGAAGT TGTGGCAATG ATTAAAGAAC TGTTAGATAC TAGAATACGG 
CCGACTGTGC AGGAGGATGG GGGTGACGTG ATCTACAGAG GCTTTGAAGA TGGCATCGTG 
CGGCTGAAGC TCCAGGGCTC CTGCACCAGC TGCCCCAGCT CTATCATCAC CCTGAAGAGC 
GGGATTCAGA ACATGCTGCA GTTTTATATT CCAGAAGTGG AAGGCGTGGA GCAGGTGATG 
GATGATGAGG AATCAGATGA AAAAGAAGCA AATTCACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100076.2
CDS	3..764
Translation	MAAAERAWGAAVGVARLCRRLCHVATPHAFKKQPLHQYVQRPLFPLRTPLYNTVRFMFIQTQDTPNPNSLKFIPGKPVLETRTMDFPTPAAAFRSPLARQLFRIEGVKSVFFGPDFITVTKENEELDWNLLKPDIYATIMDFFASGLPLVTEETPPGEAGSEEDDEVVAMIKELLDTRIRPTVQEDGGDVIYRGFEDGIVRLKLQGSCTSCPSSIITLKSGIQNMLQFYIPEVEGVEQVMDDEESDEKEANSP

Target ORF information:

RefSeq Version	NM_001106606.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NFU1 iron-sulfur cluster scaffold (Nfu1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106606.2

ORF Insert Sequence:

ATGGCGGCGG CCGAGCGGGC GTGGGGAGCA GCTGTCGGTG TAGCCCGGCT GTGCAGGCGG 
CTCTGTCATG TGGCGACTCC ACACGCCTTT AAGAAACAGC CTCTGCACCA ATATGTACAG 
AGACCACTCT TCCCTCTGCG CACGCCCTTG TATAACACAG TACGATTCAT GTTTATTCAA 
ACACAAGACA CCCCAAATCC CAACAGCTTA AAGTTTATAC CAGGAAAGCC GGTTCTTGAG 
ACGAGGACCA TGGATTTCCC CACACCAGCT GCGGCTTTCC GTTCCCCTCT GGCCAGGCAG 
TTATTCAGGA TTGAAGGTGT GAAAAGTGTC TTCTTTGGGC CAGATTTCAT TACAGTCACA 
AAGGAGAATG AAGAATTAGA CTGGAATTTA CTGAAACCAG ACATTTATGC AACAATTATG 
GACTTCTTTG CATCTGGCTT ACCTCTAGTG ACTGAGGAGA CGCCTCCAGG AGAAGCAGGT 
TCTGAGGAAG ATGACGAAGT TGTGGCAATG ATTAAAGAAC TGTTAGATAC TAGAATACGG 
CCGACTGTGC AGGAGGATGG GGGTGACGTG ATCTACAGAG GCTTTGAAGA TGGCATCGTG 
CGGCTGAAGC TCCAGGGCTC CTGCACCAGC TGCCCCAGCT CTATCATCAC CCTGAAGAGC 
GGGATTCAGA ACATGCTGCA GTTTTATATT CCAGAAGTGG AAGGCGTGGA GCAGGTGATG 
GATGATGAGG AATCAGATGA AAAAGAAGCA AATTCACCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfu1 ( XM_008763063.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761285.1 Rattus norvegicus NFU1 iron-sulfur cluster scaffold (Nfu1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa45451
Clone ID Related Accession (Same CDS sequence)	XM_008763063.2
Accession Version	XM_008763063.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NFU1 iron-sulfur cluster scaffold homolog, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763063.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTATTC AAACACAAGA CACCCCAAAT CCCAACAGCT TAAAGTTTAT ACCAGGAAAG 
CCGGTTCTTG AGACGAGGAC CATGGATTTC CCCACACCAG CTGCGGCTTT CCGTTCCCCT 
CTGGCCAGGC AGTTATTCAG GATTGAAGGT GTGAAAAGTG TCTTCTTTGG GCCAGATTTC 
ATTACAGTCA CAAAGGAGAA TGAAGAATTA GACTGGAATT TACTGAAACC AGACATTTAT 
GCAACAATTA TGGACTTCTT TGCATCTGGC TTACCTCTAG TGACTGAGGA GACGCCTCCA 
GGAGAAGCAG GTTCTGAGGA AGATGACGAA GTTGTGGCAA TGATTAAAGA ACTGTTAGAT 
ACTAGAATAC GGCCGACTGT GCAGGAGGAT GGGGGTGACG TGATCTACAG AGGCTTTGAA 
GATGGCATCG TGCGGCTGAA GCTCCAGGGC TCCTGCACCA GCTGCCCCAG CTCTATCATC 
ACCCTGAAGA GCGGGATTCA GAACATGCTG CAGTTTTATA TTCCAGAAGT GGAAGGCGTG 
GAGCAGGTGA TGGATGATGA GGAATCAGAT GAAAAAGAAG CAAATTCACC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761285.1
CDS	407..1000
Translation	MFIQTQDTPNPNSLKFIPGKPVLETRTMDFPTPAAAFRSPLARQLFRIEGVKSVFFGPDFITVTKENEELDWNLLKPDIYATIMDFFASGLPLVTEETPPGEAGSEEDDEVVAMIKELLDTRIRPTVQEDGGDVIYRGFEDGIVRLKLQGSCTSCPSSIITLKSGIQNMLQFYIPEVEGVEQVMDDEESDEKEANSP

Target ORF information:

RefSeq Version	XM_008763063.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NFU1 iron-sulfur cluster scaffold (Nfu1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763063.2

ORF Insert Sequence:

ATGTTTATTC AAACACAAGA CACCCCAAAT CCCAACAGCT TAAAGTTTAT ACCAGGAAAG 
CCGGTTCTTG AGACGAGGAC CATGGATTTC CCCACACCAG CTGCGGCTTT CCGTTCCCCT 
CTGGCCAGGC AGTTATTCAG GATTGAAGGT GTGAAAAGTG TCTTCTTTGG GCCAGATTTC 
ATTACAGTCA CAAAGGAGAA TGAAGAATTA GACTGGAATT TACTGAAACC AGACATTTAT 
GCAACAATTA TGGACTTCTT TGCATCTGGC TTACCTCTAG TGACTGAGGA GACGCCTCCA 
GGAGAAGCAG GTTCTGAGGA AGATGACGAA GTTGTGGCAA TGATTAAAGA ACTGTTAGAT 
ACTAGAATAC GGCCGACTGT GCAGGAGGAT GGGGGTGACG TGATCTACAG AGGCTTTGAA 
GATGGCATCG TGCGGCTGAA GCTCCAGGGC TCCTGCACCA GCTGCCCCAG CTCTATCATC 
ACCCTGAAGA GCGGGATTCA GAACATGCTG CAGTTTTATA TTCCAGAAGT GGAAGGCGTG 
GAGCAGGTGA TGGATGATGA GGAATCAGAT GAAAAAGAAG CAAATTCACC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Understanding the molecular basis for multiple mitochondrial dysfunctions syndrome 1 (MMDS1): impact of a disease-causing Gly189Arg substitution on NFU1.
The FEBS journal284(22)3838-3848(2017 Nov)
Wesley NA,Wachnowsky C,Fidai I,Cowan JA

Iron-sulfur cluster biogenesis in mammalian cells: New insights into the molecular mechanisms of cluster delivery.
Biochimica et biophysica acta1853(6)1493-512(2015 Jun)
Maio N,Rouault TA

The Lafora disease gene product laforin interacts with HIRIP5, a phylogenetically conserved protein containing a NifU-like domain.
Human molecular genetics12(18)2359-68(2003 Sep)
Ganesh S,Tsurutani N,Suzuki T,Ueda K,Agarwala KL,Osada H,Delgado-Escueta AV,Yamakawa K

Subcellular compartmentalization of human Nfu, an iron-sulfur cluster scaffold protein, and its ability to assemble a [4Fe-4S] cluster.
Proceedings of the National Academy of Sciences of the United States of America100(17)9762-7(2003 Aug)
Tong WH,Jameson GN,Huynh BH,Rouault TA

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Nfu1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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