Ddx18 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Gene Symbol	Ddx18
Entrez Gene ID	308490
Full Name	DEAD-box helicase 18
Synonyms	MrDb
General protein information	Preferred Names DEAD-box helicase 18 Names ATP-dependent RNA helicase DDX18 DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 Myc-regulated DEAD box protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q11

mRNA and Protein(s)

mRNA Protein Name

NM_001006996.1 NP_001006997.1 ATP-dependent RNA helicase DDX18
Pathways from BioSystems

mRNA	Protein	Name
NM_001006996.1	NP_001006997.1	ATP-dependent RNA helicase DDX18

Homologies

Homo sapiens (human)	DDX18	NP_006764.3
Bos taurus (cattle)	DDX18	XP_002685472.1
Danio rerio (zebrafish)	ddx18	NP_001003411.1
Drosophila melanogaster (fruit fly)	pit	NP_732694.2
Eremothecium gossypii	AGOS_AGR333C	NP_986999.2
Arabidopsis thaliana (thale cress)	AT3G18600	NP_188490.1
Pan troglodytes (chimpanzee)	DDX18	XP_515753.2
Canis lupus familiaris (dog)	DDX18	XP_533327.3
Mus musculus (house mouse)	Ddx18	NP_080136.2
Caenorhabditis elegans (roundworm)	B0511.6	NP_492779.1
Saccharomyces cerevisiae (baker's yeast)	HAS1	NP_014017.1
Neurospora crassa	NCU09349	XP_958928.1
Oryza sativa (rice)	Os06g0535100	NP_001057788.2
Xenopus tropicalis (tropical clawed frog)	ddx18	NP_001016776.1
Rattus norvegicus (Norway rat)	Ddx18	NP_001006997.1
Schizosaccharomyces pombe (fission yeast)	has1	NP_594488.1
Gallus gallus (chicken)	DDX18	XP_422125.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008601	XP_314700.4
Kluyveromyces lactis	KLLA0A09669g	XP_451422.1
Magnaporthe oryzae (rice blast fungus)	MGG_06522	XP_003717029.1
Arabidopsis thaliana (thale cress)	AT5G65900	NP_201391.1

Gene Ontology
Bibliography

Related articles in PubMed

DExD/H-box RNA helicases in ribosome biogenesis.
Martin R, Straub AU, Doebele C, Bohnsack MT
RNA biology10(1)4-18(2013 Jan)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Ohta S, Bukowski-Wills JC, Sanchez-Pulido L, Alves Fde L, Wood L, Chen ZA, Platani M, Fischer L, Hudson DF, Ponting CP, Fukagawa T, Earnshaw WC, Rappsilber J
Cell142(5)810-21(2010 Sep)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10953	NM_001006996.1 Latest version!	Rattus norvegicus DEAD-box helicase 18 (Ddx18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$398.30 $569.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx18 ( NM_001006996.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001006997.1 Rattus norvegicus DEAD-box helicase 18 (Ddx18), mRNA.

Price & Availability↑

CloneID	ORa10953
Clone ID Related Accession (Same CDS sequence)	NM_001006996.1
Accession Version	NM_001006996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	ATP-dependent RNA helicase DDX18
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083919.1. On Nov 22, 2004 this sequence version replaced XM_218475.2. ##Evidence-Data-START## Transcript exon combination :: BC083919.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCGCAGT TACAGATGAA ACTCCTGCGC AAGAAGATCG AAAAGCGGAA CGCCAAGCTG 
CGGCAACGGA ACCTGAAGCT GCAAGAAGCA TCAGACAGGA GCCTGTCACA GCCTGAAAAT 
GGAGATGTGC CTGAAGAACT AGGAAAAGGT GGAAAAGCTA AAAAGGCCCT GAAGCATTCT 
GTGACTGCAG GCTCAGCAGA AGCACAAAGT AGAGGCGTGC CCGAGGAGAC TGTGGAAAAT 
GCCAAAGTTA AAAAGTCACC CCAAAAACTT ACCATTTTAA CCAATGGAGA AGCAGCAGCA 
ATGCCATCTA CAGATCCAGA ACTAAAGAAG AAAAAGAAGA AGAAGAGGAA AATTGGAAAT 
GACACTGAGC CTGATCCCAA AAAAGCAAAA ACTGAAGAAG GCACGGAGGC TCGTGAGGAA 
CCAGAAGGGG CTGGTGAGGA ACCAGAAGGG GCTGGTGAGG AACCAGAAGA TGGTGTGAAG 
GAGGCAGATG ACAGTGAGGT GCCCAGTCTG CCGTTGGGAG TGACAGGTGC TTTCGAGGAC 
ACTTCGTTTG CTTCCTTGTC TAATCTCGTC AATGAGAACA CCCTGAAGGC CATAGAAGAA 
ATGGGCTTCA AGCACATGAC AGAGATCCAG CATAAAAGTA TCAGGCCACT TCTGGAAGGC 
AGGGATCTTC TGGCGGCTGC GAAAACCGGC AGCGGCAAGA CCCTTGCTTT CCTCATCCCT 
GTGATCGAGC TCATTGTCAA ATTGAAGTTC ATGCCCAGGA ATGGAACAGG AGTCCTGATT 
CTCTCTCCTA CCAGAGAACT GGCCATGCAG ACCTTTGGTG TGCTTAAGGA ACTGATGACA 
CACCACGTTC ACACGTACGG GCTGATCATG GGTGGCAGTA ACAGGTCTGC TGAGGTACAG 
AAGCTTCTCA ATGGGATCAA CATCGTCGTG GCCACCCCAG GCCGGCTCCT AGACCACATG 
CAGAACACAC CAGGGTTTAT GTACAAGAAC CTGCAGTGTC TGGTTATCGA TGAGGCCGAC 
CGTATCTTGG ATGTTGGGTT TGAAGAAGAA TTAAAGCAGA TCATTAAACT TTTGCCAGCA 
CGTAGGCAAA CCATGCTCTT CTCTGCCACA CAAACTCGAA AAGTTGAAGA CTTGGCAAGA 
ATTTCCCTGA AAAAAGAGCC ATTGTACGTG GGTGTTGACG ACGACAAAGA GGTTGCCACA 
GTGGATGGCC TTGAGCAGGG GTATGTTGTC TGTCCCTCTG AGAAGAGGTT CCTTCTGCTT 
TTCACATTCC TTAAGAAGAA CCGGAAGAAG AAAGTGATGG TCTTCTTTTC CTCCTGTATG 
TCTGTGAAAT ACCACTATGA GCTGCTGAAC TACATTGACC TGCCTGTCTT AGCCATCCAT 
GGAAAGCAAA AGCAAAATAA GCGAACAACC ACGTTCTTCC AATTCTGCAA CGCAGATTCG 
GGAATACTGC TGTGCACTGA TGTGGCGGCC AGAGGGCTGG ACATCCCTGA AGTGGACTGG 
ATTGTTCAGT ACGATCCTCC CGATGACCCC AAGGAGTACA TTCATCGGGT CGGCAGAACA 
GCCCGAGGCC TGAATGGAAG AGGGCACGCC CTGCTCATCT TGCGCCCTGA AGAGCTGGGT 
TTCCTTCGGT ACTTGAAACA ATCCAAGGTT CCGTTGAATC AGTTTGACTT CTCCTGGTCT 
AAGGTTTCTG ATATTCAGTC TCAGCTGGAA AAACTGATCG AAAAGAACTA CTTTCTGCAC 
AAGTCTGCCC AGGAAGCGTA CAAGTCCTAC ATACGGGCAT ATGACTCCCA CTCTCTCAAG 
CAGATCTTCA ATGTCAACAA CTTAAATTTG CCTCAAGTTG CTCTGTCTTT TGGTTTTAAG 
GTCCCTCCTT TTGTTGATCT GAATGTGAGC AGCCACGACG GCAAGCTTAA AAAGAGAGGT 
GGTGGTGGGG GATTCGGCTA CCAGAAAACA AAGAAAGTGG AGAAGTCCAA GATCTTTAAA 
CACATCAGTA AGAAGCCAGC AGACCGCAGG CAGTTCTCTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006997.1
CDS	34..2058
Translation	MSQLQMKLLRKKIEKRNAKLRQRNLKLQEASDRSLSQPENGDVPEELGKGGKAKKALKHSVTAGSAEAQSRGVPEETVENAKVKKSPQKLTILTNGEAAAMPSTDPELKKKKKKKRKIGNDTEPDPKKAKTEEGTEAREEPEGAGEEPEGAGEEPEDGVKEADDSEVPSLPLGVTGAFEDTSFASLSNLVNENTLKAIEEMGFKHMTEIQHKSIRPLLEGRDLLAAAKTGSGKTLAFLIPVIELIVKLKFMPRNGTGVLILSPTRELAMQTFGVLKELMTHHVHTYGLIMGGSNRSAEVQKLLNGINIVVATPGRLLDHMQNTPGFMYKNLQCLVIDEADRILDVGFEEELKQIIKLLPARRQTMLFSATQTRKVEDLARISLKKEPLYVGVDDDKEVATVDGLEQGYVVCPSEKRFLLLFTFLKKNRKKKVMVFFSSCMSVKYHYELLNYIDLPVLAIHGKQKQNKRTTTFFQFCNADSGILLCTDVAARGLDIPEVDWIVQYDPPDDPKEYIHRVGRTARGLNGRGHALLILRPEELGFLRYLKQSKVPLNQFDFSWSKVSDIQSQLEKLIEKNYFLHKSAQEAYKSYIRAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVSSHDGKLKKRGGGGGFGYQKTKKVEKSKIFKHISKKPADRRQFSH

Target ORF information:

RefSeq Version	NM_001006996.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DEAD-box helicase 18 (Ddx18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006996.1

ORF Insert Sequence:

ATGTCGCAGT TACAGATGAA ACTCCTGCGC AAGAAGATCG AAAAGCGGAA CGCCAAGCTG 
CGGCAACGGA ACCTGAAGCT GCAAGAAGCA TCAGACAGGA GCCTGTCACA GCCTGAAAAT 
GGAGATGTGC CTGAAGAACT AGGAAAAGGT GGAAAAGCTA AAAAGGCCCT GAAGCATTCT 
GTGACTGCAG GCTCAGCAGA AGCACAAAGT AGAGGCGTGC CCGAGGAGAC TGTGGAAAAT 
GCCAAAGTTA AAAAGTCACC CCAAAAACTT ACCATTTTAA CCAATGGAGA AGCAGCAGCA 
ATGCCATCTA CAGATCCAGA ACTAAAGAAG AAAAAGAAGA AGAAGAGGAA AATTGGAAAT 
GACACTGAGC CTGATCCCAA AAAAGCAAAA ACTGAAGAAG GCACGGAGGC TCGTGAGGAA 
CCAGAAGGGG CTGGTGAGGA ACCAGAAGGG GCTGGTGAGG AACCAGAAGA TGGTGTGAAG 
GAGGCAGATG ACAGTGAGGT GCCCAGTCTG CCGTTGGGAG TGACAGGTGC TTTCGAGGAC 
ACTTCGTTTG CTTCCTTGTC TAATCTCGTC AATGAGAACA CCCTGAAGGC CATAGAAGAA 
ATGGGCTTCA AGCACATGAC AGAGATCCAG CATAAAAGTA TCAGGCCACT TCTGGAAGGC 
AGGGATCTTC TGGCGGCTGC GAAAACCGGC AGCGGCAAGA CCCTTGCTTT CCTCATCCCT 
GTGATCGAGC TCATTGTCAA ATTGAAGTTC ATGCCCAGGA ATGGAACAGG AGTCCTGATT 
CTCTCTCCTA CCAGAGAACT GGCCATGCAG ACCTTTGGTG TGCTTAAGGA ACTGATGACA 
CACCACGTTC ACACGTACGG GCTGATCATG GGTGGCAGTA ACAGGTCTGC TGAGGTACAG 
AAGCTTCTCA ATGGGATCAA CATCGTCGTG GCCACCCCAG GCCGGCTCCT AGACCACATG 
CAGAACACAC CAGGGTTTAT GTACAAGAAC CTGCAGTGTC TGGTTATCGA TGAGGCCGAC 
CGTATCTTGG ATGTTGGGTT TGAAGAAGAA TTAAAGCAGA TCATTAAACT TTTGCCAGCA 
CGTAGGCAAA CCATGCTCTT CTCTGCCACA CAAACTCGAA AAGTTGAAGA CTTGGCAAGA 
ATTTCCCTGA AAAAAGAGCC ATTGTACGTG GGTGTTGACG ACGACAAAGA GGTTGCCACA 
GTGGATGGCC TTGAGCAGGG GTATGTTGTC TGTCCCTCTG AGAAGAGGTT CCTTCTGCTT 
TTCACATTCC TTAAGAAGAA CCGGAAGAAG AAAGTGATGG TCTTCTTTTC CTCCTGTATG 
TCTGTGAAAT ACCACTATGA GCTGCTGAAC TACATTGACC TGCCTGTCTT AGCCATCCAT 
GGAAAGCAAA AGCAAAATAA GCGAACAACC ACGTTCTTCC AATTCTGCAA CGCAGATTCG 
GGAATACTGC TGTGCACTGA TGTGGCGGCC AGAGGGCTGG ACATCCCTGA AGTGGACTGG 
ATTGTTCAGT ACGATCCTCC CGATGACCCC AAGGAGTACA TTCATCGGGT CGGCAGAACA 
GCCCGAGGCC TGAATGGAAG AGGGCACGCC CTGCTCATCT TGCGCCCTGA AGAGCTGGGT 
TTCCTTCGGT ACTTGAAACA ATCCAAGGTT CCGTTGAATC AGTTTGACTT CTCCTGGTCT 
AAGGTTTCTG ATATTCAGTC TCAGCTGGAA AAACTGATCG AAAAGAACTA CTTTCTGCAC 
AAGTCTGCCC AGGAAGCGTA CAAGTCCTAC ATACGGGCAT ATGACTCCCA CTCTCTCAAG 
CAGATCTTCA ATGTCAACAA CTTAAATTTG CCTCAAGTTG CTCTGTCTTT TGGTTTTAAG 
GTCCCTCCTT TTGTTGATCT GAATGTGAGC AGCCACGACG GCAAGCTTAA AAAGAGAGGT 
GGTGGTGGGG GATTCGGCTA CCAGAAAACA AAGAAAGTGG AGAAGTCCAA GATCTTTAAA 
CACATCAGTA AGAAGCCAGC AGACCGCAGG CAGTTCTCTC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DExD/H-box RNA helicases in ribosome biogenesis.
RNA biology10(1)4-18(2013 Jan)
Martin R,Straub AU,Doebele C,Bohnsack MT

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

The protein composition of mitotic chromosomes determined using multiclassifier combinatorial proteomics.
Cell142(5)810-21(2010 Sep)
Ohta S,Bukowski-Wills JC,Sanchez-Pulido L,Alves Fde L,Wood L,Chen ZA,Platani M,Fischer L,Hudson DF,Ponting CP,Fukagawa T,Earnshaw WC,Rappsilber J

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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Ddx18 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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