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Pde8a cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Pde8a
Entrez Gene ID 308776
Full Name phosphodiesterase 8A
Synonyms RNPDE8A
General protein information
Preferred Names

phosphodiesterase 8A

Names

high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
cAMP-specific cyclic nucleotide phosphodiesterase PDE8
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

1

1q31
Summary mouse homolog is a cAMP-specific cyclic nucleotide phosphodiesterase [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006229438.3 XP_006229500.1 high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X1
NM_198767.1 NP_942062.1 high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
XM_006229439.3 XP_006229501.1 high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X2

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) PDE8A XP_001163046.1
Homo sapiens (human) PDE8A NP_002596.1
Mus musculus (house mouse) Pde8a NP_032829.1
Caenorhabditis elegans (roundworm) pde-6 NP_490787.1
PDE8A XP_001086066.1
Rattus norvegicus (Norway rat) Pde8a NP_942062.1
Danio rerio (zebrafish) pde8a NP_001074096.1
Canis lupus familiaris (dog) PDE8A XP_005618438.1
Xenopus tropicalis (tropical clawed frog) pde8a XP_002939668.2
Bos taurus (cattle) PDE8A NP_001178384.1
Gallus gallus (chicken) PDE8A XP_413834.2

Gene Ontology


Bibliography

Related articles in PubMed

Phosphodiesterase-8A binds to and regulates Raf-1 kinase.
Brown KM, Day JP, Huston E, Zimmermann B, Hampel K, Christian F, Romano D, Terhzaz S, Lee LC, Willis MJ, Morton DB, Beavo JA, Shimizu-Albergine M, Davies SA, Kolch W, Houslay MD, Baillie GS
Proceedings of the National Academy of Sciences of the United States of America110(16)E1533-42(2013 Apr)

Distribution of PDE8A in the nervous system of the Sprague-Dawley rat.
Kruse LS, M?ller M, Kruuse C
Journal of chemical neuroanatomy42(3)184-91(2011 Nov)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signaling.
Conti M, Beavo J
Annual review of biochemistry76481-511(2007)

Molecular comparison of rat cyclic nucleotide phosphodiesterase 8 family: unique expression of PDE8B in rat brain.
Kobayashi T, Gamanuma M, Sasaki T, Yamashita Y, Yuasa K, Kotera J, Omori K
Gene31921-31(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pde8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa05015 NM_198767.1
Latest version!		 Rattus norvegicus phosphodiesterase 8A (Pde8a), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
ORa05015 XM_006229438.3
Latest version!		 Rattus norvegicus phosphodiesterase 8A (Pde8a), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
ORa22944 XM_006229439.3
Latest version!		 Rattus norvegicus phosphodiesterase 8A (Pde8a), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa05015
Clone ID Related Accession (Same CDS sequence) XM_006229438.3 , NM_198767.1
Accession Version NM_198767.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-18
Organism Rattus norvegicus(Norway rat)
Product high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB092696.1. On Dec 17, 2003 this sequence version replaced XM_218849.2. ##Evidence-Data-START## Transcript exon combination :: AB092696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGGCTGCG CCCCGAGCAT CCACATTTCC GACAACCGAG TGTCGAACCC CAGCGACAGC 
GACGGCGAGG ACGAGGACGT CCGGGTCCCC GCGCTGAGGT CTAGACAGCG ATGGTCGACC 
CCGCCCGGGC TCGTGGAGCC TCAGCCTCGC GACAGCGGTG CCTCGAAGGT ATCAGTTGCT 
GATGTGCAAT TTGGCCCGAT GAGATTTCAT CAAGACCAGC TTCAGGTACT TTTAGTGTTT 
ACCAAAGAAG ATAGCCAGTG TAATGGATTC TACAGGGCAT GTGAAAAAGC CGGGTTTAAG 
TGCACAGTCA CCAAGGAGGT GCAGACTGTC CTTGCCTGTT TCCAGGACAA ACTCCATGAC 
ATCATCATCA TAGACCACAG AAATCCCCGA CAGATGGATG CGGAGGCAAT GTGCAGGTCT 
ATCAGATCAT CAAAATTATC AGAAAACACA GTTATTGTTG GCGTAGTACG CAGGGTGGAC 
AAAGAAGAGG TGTCTTTAAT GCCCTTCCTT GCCGCTGGCT TCACGCGGAG GTATATAGAA 
AACCCCAACG TCATGGCCTG TTATAATGAA CTGCTCCAGT TGGCATTTGG AGAGGTGCGA 
TCACAGCTGA AACTCAGGGC TTGTAACTCA GTGTTCACTG CGTTAGAGAA GAGTCAAGAA 
GCGATTGAGA TCACAAGTGA GGACCACATC ATCCAGTATG CAAACCCTGC ATTTGAAACA 
ACAATGGGTT ATCAGTCGGG AGAACTAATA GGGAAGGAGT TAGCACAAGT GCCCATCAAT 
GAGAAGAAAG GTGACTTGCT GGATGCCATA AATTCCTGTG TCACAATCGA CAAGGAGTGG 
CAAGGAGTTT ACCATACCCA AAAGAAAAAT GGAGATAACA TCCAACAAAA TGTGAAGATA 
ATACCTGTCA TTGGGCAGGG AGGAAAAATC AGACACTATG TGTCCATTAT CAGAGTGTGC 
AATGGCAGCA ATAAGGTTGA GACCACAACT GAGTGTGTTC AGACTGATAG TCAAACAGAT 
AGTCAGGCAG GCAAGCATAA AGACAGGAGG AAACATTCGA TGGACGTCAA AGCGGTTTCC 
TCCAGAACAA GCGAAGTTTC CAATCAGAGA AGACATTCCT CTCTGGCCCG GATACATTCT 
ATGATGATTG AGGCACCAAT CACCAAGGTA ATCAATATTC TTAGTGCTGC CCAGGAAAAC 
AGTCCTGTGT CTGTGACAGA AGCCTTGAAC CATGTGCTGG ATATCCTGAG AACCACTGAA 
CTGTATTCAC CCCAGTTTAA TACTGAGGAT GACCCTCACG CCACTGACCT CGTTGGGGGC 
TTAATGTCTG ATGGCTTACG GAGGTTTTCA GGAAATGAGT ATACTCTTGC AACAAAAAGC 
CTTCGGCCAT CTTCAAGCCA GTTGGCCGTC CCCATCTCCC TTCATGATAT CCCACCACGG 
ATTGCTCAGG TCATAGAAAA TGAGGAGCAA TGGGACTTTG ACATTTTTGA GCTGGAAGCT 
GCAACCCAAA ATAGGCCTTT GATTTATCTA GGTCTCAAAA CGTTTGCTCG TTTTGAAATC 
TGTGAGTTCT TACAGTGCTC TGAGGCAACG CTGAAATCAT GGTTTCAAAT TATTGAATCC 
AATTACCATT CCTCTAACCC CTACCACAAT TCCACGCACG CCGCGGATGT GCTTCACGCC 
ACTGCGTATT TCCTTTCCAG AAACAAGTTA AAGGACACTT TAGATCGCCT TGATGAGGTC 
GCTGCCCTCA TCGCTGCCAC CATCCACGAT GTGGATCACC CCGGGAGAAC CAACTCTTTC 
CTCTGCAATG CGGGAAAGGA GCTGGCCGTA CTGTACAATG ACACTGCTGT GCTAGAGAGC 
CACCACGCAG CGCTGGCCTT CCAGCTGACC CTTGAAAATG ACAAATGCAA TATCTTTAAA 
AACACAGACA GGAATGACTA CCGGACACTA CGCCAGAGTA TCATTGACAT GGTCCTAGCC 
ACAGAGATGA CAAAGCACTT TGAACACGTC AACAAATTTA TCAACAGCAT CAACAAGCCT 
CTGAAAGCAC AGAAAAACGA GGTTCAGGAA ACTGGCAGAA GTCTGGAAGA TATAAGAGCC 
ATGGTCAGGA CTCCAGAGAG CCGGACTCTC ATCAAAAGGA TGATGATTAA GTGTGCCGAC 
GTGTCCAACC CCTGCCGGCC CCTCGAGCAC TGCATCGAGT GGGCTGCACG CATCTCAGAG 
GAGTATTTCT CCCAGACCGA TGAAGAGAAA CAGCTGGACT TGCCTGTGGT GATGCCAGTG 
TTTGACAGAA ACACCTGCAG CATCCCCAAG TCTCAGATCT CCTTCATCGA CTACTTCATC 
ACAGACATGT TTGATGCCTG GGATGCCTTT GTTTACCTGC CTAACCTTAT GCGGCACCTG 
GAGGACAACT TCAAGTACTG GAAAGGACTG GATGAGAAGG AGCTGTGCAC CCTCCGGCCC 
CCTCCAGAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_942062.1
CDS63..2534
Translation

Target ORF information:

RefSeq Version NM_198767.1
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphodiesterase 8A (Pde8a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_198767.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGGCTGCG CCCCGAGCAT CCACATTTCC GACAACCGAG TGTCGAACCC CAGCGACAGC 
GACGGCGAGG ACGAGGACGT CCGGGTCCCC GCGCTGAGGT CTAGACAGCG ATGGTCGACC 
CCGCCCGGGC TCGTGGAGCC TCAGCCTCGC GACAGCGGTG CCTCGAAGGT ATCAGTTGCT 
GATGTGCAAT TTGGCCCGAT GAGATTTCAT CAAGACCAGC TTCAGGTACT TTTAGTGTTT 
ACCAAAGAAG ATAGCCAGTG TAATGGATTC TACAGGGCAT GTGAAAAAGC CGGGTTTAAG 
TGCACAGTCA CCAAGGAGGT GCAGACTGTC CTTGCCTGTT TCCAGGACAA ACTCCATGAC 
ATCATCATCA TAGACCACAG AAATCCCCGA CAGATGGATG CGGAGGCAAT GTGCAGGTCT 
ATCAGATCAT CAAAATTATC AGAAAACACA GTTATTGTTG GCGTAGTACG CAGGGTGGAC 
AAAGAAGAGG TGTCTTTAAT GCCCTTCCTT GCCGCTGGCT TCACGCGGAG GTATATAGAA 
AACCCCAACG TCATGGCCTG TTATAATGAA CTGCTCCAGT TGGCATTTGG AGAGGTGCGA 
TCACAGCTGA AACTCAGGGC TTGTAACTCA GTGTTCACTG CGTTAGAGAA GAGTCAAGAA 
GCGATTGAGA TCACAAGTGA GGACCACATC ATCCAGTATG CAAACCCTGC ATTTGAAACA 
ACAATGGGTT ATCAGTCGGG AGAACTAATA GGGAAGGAGT TAGCACAAGT GCCCATCAAT 
GAGAAGAAAG GTGACTTGCT GGATGCCATA AATTCCTGTG TCACAATCGA CAAGGAGTGG 
CAAGGAGTTT ACCATACCCA AAAGAAAAAT GGAGATAACA TCCAACAAAA TGTGAAGATA 
ATACCTGTCA TTGGGCAGGG AGGAAAAATC AGACACTATG TGTCCATTAT CAGAGTGTGC 
AATGGCAGCA ATAAGGTTGA GACCACAACT GAGTGTGTTC AGACTGATAG TCAAACAGAT 
AGTCAGGCAG GCAAGCATAA AGACAGGAGG AAACATTCGA TGGACGTCAA AGCGGTTTCC 
TCCAGAACAA GCGAAGTTTC CAATCAGAGA AGACATTCCT CTCTGGCCCG GATACATTCT 
ATGATGATTG AGGCACCAAT CACCAAGGTA ATCAATATTC TTAGTGCTGC CCAGGAAAAC 
AGTCCTGTGT CTGTGACAGA AGCCTTGAAC CATGTGCTGG ATATCCTGAG AACCACTGAA 
CTGTATTCAC CCCAGTTTAA TACTGAGGAT GACCCTCACG CCACTGACCT CGTTGGGGGC 
TTAATGTCTG ATGGCTTACG GAGGTTTTCA GGAAATGAGT ATACTCTTGC AACAAAAAGC 
CTTCGGCCAT CTTCAAGCCA GTTGGCCGTC CCCATCTCCC TTCATGATAT CCCACCACGG 
ATTGCTCAGG TCATAGAAAA TGAGGAGCAA TGGGACTTTG ACATTTTTGA GCTGGAAGCT 
GCAACCCAAA ATAGGCCTTT GATTTATCTA GGTCTCAAAA CGTTTGCTCG TTTTGAAATC 
TGTGAGTTCT TACAGTGCTC TGAGGCAACG CTGAAATCAT GGTTTCAAAT TATTGAATCC 
AATTACCATT CCTCTAACCC CTACCACAAT TCCACGCACG CCGCGGATGT GCTTCACGCC 
ACTGCGTATT TCCTTTCCAG AAACAAGTTA AAGGACACTT TAGATCGCCT TGATGAGGTC 
GCTGCCCTCA TCGCTGCCAC CATCCACGAT GTGGATCACC CCGGGAGAAC CAACTCTTTC 
CTCTGCAATG CGGGAAAGGA GCTGGCCGTA CTGTACAATG ACACTGCTGT GCTAGAGAGC 
CACCACGCAG CGCTGGCCTT CCAGCTGACC CTTGAAAATG ACAAATGCAA TATCTTTAAA 
AACACAGACA GGAATGACTA CCGGACACTA CGCCAGAGTA TCATTGACAT GGTCCTAGCC 
ACAGAGATGA CAAAGCACTT TGAACACGTC AACAAATTTA TCAACAGCAT CAACAAGCCT 
CTGAAAGCAC AGAAAAACGA GGTTCAGGAA ACTGGCAGAA GTCTGGAAGA TATAAGAGCC 
ATGGTCAGGA CTCCAGAGAG CCGGACTCTC ATCAAAAGGA TGATGATTAA GTGTGCCGAC 
GTGTCCAACC CCTGCCGGCC CCTCGAGCAC TGCATCGAGT GGGCTGCACG CATCTCAGAG 
GAGTATTTCT CCCAGACCGA TGAAGAGAAA CAGCTGGACT TGCCTGTGGT GATGCCAGTG 
TTTGACAGAA ACACCTGCAG CATCCCCAAG TCTCAGATCT CCTTCATCGA CTACTTCATC 
ACAGACATGT TTGATGCCTG GGATGCCTTT GTTTACCTGC CTAACCTTAT GCGGCACCTG 
GAGGACAACT TCAAGTACTG GAAAGGACTG GATGAGAAGG AGCTGTGCAC CCTCCGGCCC 
CCTCCAGAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa05015
Clone ID Related Accession (Same CDS sequence) XM_006229438.3 , NM_198767.1
Accession Version XM_006229438.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGGCTGCG CCCCGAGCAT CCACATTTCC GACAACCGAG TGTCGAACCC CAGCGACAGC 
GACGGCGAGG ACGAGGACGT CCGGGTCCCC GCGCTGAGGT CTAGACAGCG ATGGTCGACC 
CCGCCCGGGC TCGTGGAGCC TCAGCCTCGC GACAGCGGTG CCTCGAAGGT ATCAGTTGCT 
GATGTGCAAT TTGGCCCGAT GAGATTTCAT CAAGACCAGC TTCAGGTACT TTTAGTGTTT 
ACCAAAGAAG ATAGCCAGTG TAATGGATTC TACAGGGCAT GTGAAAAAGC CGGGTTTAAG 
TGCACAGTCA CCAAGGAGGT GCAGACTGTC CTTGCCTGTT TCCAGGACAA ACTCCATGAC 
ATCATCATCA TAGACCACAG AAATCCCCGA CAGATGGATG CGGAGGCAAT GTGCAGGTCT 
ATCAGATCAT CAAAATTATC AGAAAACACA GTTATTGTTG GCGTAGTACG CAGGGTGGAC 
AAAGAAGAGG TGTCTTTAAT GCCCTTCCTT GCCGCTGGCT TCACGCGGAG GTATATAGAA 
AACCCCAACG TCATGGCCTG TTATAATGAA CTGCTCCAGT TGGCATTTGG AGAGGTGCGA 
TCACAGCTGA AACTCAGGGC TTGTAACTCA GTGTTCACTG CGTTAGAGAA GAGTCAAGAA 
GCGATTGAGA TCACAAGTGA GGACCACATC ATCCAGTATG CAAACCCTGC ATTTGAAACA 
ACAATGGGTT ATCAGTCGGG AGAACTAATA GGGAAGGAGT TAGCACAAGT GCCCATCAAT 
GAGAAGAAAG GTGACTTGCT GGATGCCATA AATTCCTGTG TCACAATCGA CAAGGAGTGG 
CAAGGAGTTT ACCATACCCA AAAGAAAAAT GGAGATAACA TCCAACAAAA TGTGAAGATA 
ATACCTGTCA TTGGGCAGGG AGGAAAAATC AGACACTATG TGTCCATTAT CAGAGTGTGC 
AATGGCAGCA ATAAGGTTGA GACCACAACT GAGTGTGTTC AGACTGATAG TCAAACAGAT 
AGTCAGGCAG GCAAGCATAA AGACAGGAGG AAACATTCGA TGGACGTCAA AGCGGTTTCC 
TCCAGAACAA GCGAAGTTTC CAATCAGAGA AGACATTCCT CTCTGGCCCG GATACATTCT 
ATGATGATTG AGGCACCAAT CACCAAGGTA ATCAATATTC TTAGTGCTGC CCAGGAAAAC 
AGTCCTGTGT CTGTGACAGA AGCCTTGAAC CATGTGCTGG ATATCCTGAG AACCACTGAA 
CTGTATTCAC CCCAGTTTAA TACTGAGGAT GACCCTCACG CCACTGACCT CGTTGGGGGC 
TTAATGTCTG ATGGCTTACG GAGGTTTTCA GGAAATGAGT ATACTCTTGC AACAAAAAGC 
CTTCGGCCAT CTTCAAGCCA GTTGGCCGTC CCCATCTCCC TTCATGATAT CCCACCACGG 
ATTGCTCAGG TCATAGAAAA TGAGGAGCAA TGGGACTTTG ACATTTTTGA GCTGGAAGCT 
GCAACCCAAA ATAGGCCTTT GATTTATCTA GGTCTCAAAA CGTTTGCTCG TTTTGAAATC 
TGTGAGTTCT TACAGTGCTC TGAGGCAACG CTGAAATCAT GGTTTCAAAT TATTGAATCC 
AATTACCATT CCTCTAACCC CTACCACAAT TCCACGCACG CCGCGGATGT GCTTCACGCC 
ACTGCGTATT TCCTTTCCAG AAACAAGTTA AAGGACACTT TAGATCGCCT TGATGAGGTC 
GCTGCCCTCA TCGCTGCCAC CATCCACGAT GTGGATCACC CCGGGAGAAC CAACTCTTTC 
CTCTGCAATG CGGGAAAGGA GCTGGCCGTA CTGTACAATG ACACTGCTGT GCTAGAGAGC 
CACCACGCAG CGCTGGCCTT CCAGCTGACC CTTGAAAATG ACAAATGCAA TATCTTTAAA 
AACACAGACA GGAATGACTA CCGGACACTA CGCCAGAGTA TCATTGACAT GGTCCTAGCC 
ACAGAGATGA CAAAGCACTT TGAACACGTC AACAAATTTA TCAACAGCAT CAACAAGCCT 
CTGAAAGCAC AGAAAAACGA GGTTCAGGAA ACTGGCAGAA GTCTGGAAGA TATAAGAGCC 
ATGGTCAGGA CTCCAGAGAG CCGGACTCTC ATCAAAAGGA TGATGATTAA GTGTGCCGAC 
GTGTCCAACC CCTGCCGGCC CCTCGAGCAC TGCATCGAGT GGGCTGCACG CATCTCAGAG 
GAGTATTTCT CCCAGACCGA TGAAGAGAAA CAGCTGGACT TGCCTGTGGT GATGCCAGTG 
TTTGACAGAA ACACCTGCAG CATCCCCAAG TCTCAGATCT CCTTCATCGA CTACTTCATC 
ACAGACATGT TTGATGCCTG GGATGCCTTT GTTTACCTGC CTAACCTTAT GCGGCACCTG 
GAGGACAACT TCAAGTACTG GAAAGGACTG GATGAGAAGG AGCTGTGCAC CCTCCGGCCC 
CCTCCAGAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006229500.1
CDS195..2666
Translation

Target ORF information:

RefSeq Version XM_006229438.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphodiesterase 8A (Pde8a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006229438.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
ATGGGCTGCG CCCCGAGCAT CCACATTTCC GACAACCGAG TGTCGAACCC CAGCGACAGC 
GACGGCGAGG ACGAGGACGT CCGGGTCCCC GCGCTGAGGT CTAGACAGCG ATGGTCGACC 
CCGCCCGGGC TCGTGGAGCC TCAGCCTCGC GACAGCGGTG CCTCGAAGGT ATCAGTTGCT 
GATGTGCAAT TTGGCCCGAT GAGATTTCAT CAAGACCAGC TTCAGGTACT TTTAGTGTTT 
ACCAAAGAAG ATAGCCAGTG TAATGGATTC TACAGGGCAT GTGAAAAAGC CGGGTTTAAG 
TGCACAGTCA CCAAGGAGGT GCAGACTGTC CTTGCCTGTT TCCAGGACAA ACTCCATGAC 
ATCATCATCA TAGACCACAG AAATCCCCGA CAGATGGATG CGGAGGCAAT GTGCAGGTCT 
ATCAGATCAT CAAAATTATC AGAAAACACA GTTATTGTTG GCGTAGTACG CAGGGTGGAC 
AAAGAAGAGG TGTCTTTAAT GCCCTTCCTT GCCGCTGGCT TCACGCGGAG GTATATAGAA 
AACCCCAACG TCATGGCCTG TTATAATGAA CTGCTCCAGT TGGCATTTGG AGAGGTGCGA 
TCACAGCTGA AACTCAGGGC TTGTAACTCA GTGTTCACTG CGTTAGAGAA GAGTCAAGAA 
GCGATTGAGA TCACAAGTGA GGACCACATC ATCCAGTATG CAAACCCTGC ATTTGAAACA 
ACAATGGGTT ATCAGTCGGG AGAACTAATA GGGAAGGAGT TAGCACAAGT GCCCATCAAT 
GAGAAGAAAG GTGACTTGCT GGATGCCATA AATTCCTGTG TCACAATCGA CAAGGAGTGG 
CAAGGAGTTT ACCATACCCA AAAGAAAAAT GGAGATAACA TCCAACAAAA TGTGAAGATA 
ATACCTGTCA TTGGGCAGGG AGGAAAAATC AGACACTATG TGTCCATTAT CAGAGTGTGC 
AATGGCAGCA ATAAGGTTGA GACCACAACT GAGTGTGTTC AGACTGATAG TCAAACAGAT 
AGTCAGGCAG GCAAGCATAA AGACAGGAGG AAACATTCGA TGGACGTCAA AGCGGTTTCC 
TCCAGAACAA GCGAAGTTTC CAATCAGAGA AGACATTCCT CTCTGGCCCG GATACATTCT 
ATGATGATTG AGGCACCAAT CACCAAGGTA ATCAATATTC TTAGTGCTGC CCAGGAAAAC 
AGTCCTGTGT CTGTGACAGA AGCCTTGAAC CATGTGCTGG ATATCCTGAG AACCACTGAA 
CTGTATTCAC CCCAGTTTAA TACTGAGGAT GACCCTCACG CCACTGACCT CGTTGGGGGC 
TTAATGTCTG ATGGCTTACG GAGGTTTTCA GGAAATGAGT ATACTCTTGC AACAAAAAGC 
CTTCGGCCAT CTTCAAGCCA GTTGGCCGTC CCCATCTCCC TTCATGATAT CCCACCACGG 
ATTGCTCAGG TCATAGAAAA TGAGGAGCAA TGGGACTTTG ACATTTTTGA GCTGGAAGCT 
GCAACCCAAA ATAGGCCTTT GATTTATCTA GGTCTCAAAA CGTTTGCTCG TTTTGAAATC 
TGTGAGTTCT TACAGTGCTC TGAGGCAACG CTGAAATCAT GGTTTCAAAT TATTGAATCC 
AATTACCATT CCTCTAACCC CTACCACAAT TCCACGCACG CCGCGGATGT GCTTCACGCC 
ACTGCGTATT TCCTTTCCAG AAACAAGTTA AAGGACACTT TAGATCGCCT TGATGAGGTC 
GCTGCCCTCA TCGCTGCCAC CATCCACGAT GTGGATCACC CCGGGAGAAC CAACTCTTTC 
CTCTGCAATG CGGGAAAGGA GCTGGCCGTA CTGTACAATG ACACTGCTGT GCTAGAGAGC 
CACCACGCAG CGCTGGCCTT CCAGCTGACC CTTGAAAATG ACAAATGCAA TATCTTTAAA 
AACACAGACA GGAATGACTA CCGGACACTA CGCCAGAGTA TCATTGACAT GGTCCTAGCC 
ACAGAGATGA CAAAGCACTT TGAACACGTC AACAAATTTA TCAACAGCAT CAACAAGCCT 
CTGAAAGCAC AGAAAAACGA GGTTCAGGAA ACTGGCAGAA GTCTGGAAGA TATAAGAGCC 
ATGGTCAGGA CTCCAGAGAG CCGGACTCTC ATCAAAAGGA TGATGATTAA GTGTGCCGAC 
GTGTCCAACC CCTGCCGGCC CCTCGAGCAC TGCATCGAGT GGGCTGCACG CATCTCAGAG 
GAGTATTTCT CCCAGACCGA TGAAGAGAAA CAGCTGGACT TGCCTGTGGT GATGCCAGTG 
TTTGACAGAA ACACCTGCAG CATCCCCAAG TCTCAGATCT CCTTCATCGA CTACTTCATC 
ACAGACATGT TTGATGCCTG GGATGCCTTT GTTTACCTGC CTAACCTTAT GCGGCACCTG 
GAGGACAACT TCAAGTACTG GAAAGGACTG GATGAGAAGG AGCTGTGCAC CCTCCGGCCC 
CCTCCAGAAT AG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa22944
Clone ID Related Accession (Same CDS sequence) XM_006229439.3
Accession Version XM_006229439.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 2271bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGGCTGCG CCCCGAGCAT CCACATTTCC GACAACCGAG TGTCGAACCC CAGCGACAGC 
GACGGCGAGG ACGAGGACGT CCGGGTCCCC GCGCTGAGGT CTAGACAGCG ATGGTCGACC 
CCGCCCGGGC TCGTGGAGCC TCAGCCTCGC GACAGCGGTG CCTCGAAGGT ATCAGTTGCT 
GATGTGCAAT TTGGCCCGAT GAGATTTCAT CAAGACCAGC TTCAGGTACT TTTAGTGTTT 
ACCAAAGAAG ATAGCCAGTG TAATGGATTC TACAGGGCAT GTGAAAAAGC CGGGTTTAAG 
TGCACAGTCA CCAAGGAGGT GCAGACTGTC CTTGCCTGTT TCCAGGACAA ACTCCATGAC 
ATCATCATCA TAGACCACAG AAATCCCCGA CAGATGGATG CGGAGGCAAT GTGCAGGGCT 
TGTAACTCAG TGTTCACTGC GTTAGAGAAG AGTCAAGAAG CGATTGAGAT CACAAGTGAG 
GACCACATCA TCCAGTATGC AAACCCTGCA TTTGAAACAA CAATGGGTTA TCAGTCGGGA 
GAACTAATAG GGAAGGAGTT AGCACAAGTG CCCATCAATG AGAAGAAAGG TGACTTGCTG 
GATGCCATAA ATTCCTGTGT CACAATCGAC AAGGAGTGGC AAGGAGTTTA CCATACCCAA 
AAGAAAAATG GAGATAACAT CCAACAAAAT GTGAAGATAA TACCTGTCAT TGGGCAGGGA 
GGAAAAATCA GACACTATGT GTCCATTATC AGAGTGTGCA ATGGCAGCAA TAAGGTTGAG 
ACCACAACTG AGTGTGTTCA GACTGATAGT CAAACAGATA GTCAGGCAGG CAAGCATAAA 
GACAGGAGGA AACATTCGAT GGACGTCAAA GCGGTTTCCT CCAGAACAAG CGAAGTTTCC 
AATCAGAGAA GACATTCCTC TCTGGCCCGG ATACATTCTA TGATGATTGA GGCACCAATC 
ACCAAGGTAA TCAATATTCT TAGTGCTGCC CAGGAAAACA GTCCTGTGTC TGTGACAGAA 
GCCTTGAACC ATGTGCTGGA TATCCTGAGA ACCACTGAAC TGTATTCACC CCAGTTTAAT 
ACTGAGGATG ACCCTCACGC CACTGACCTC GTTGGGGGCT TAATGTCTGA TGGCTTACGG 
AGGTTTTCAG GAAATGAGTA TACTCTTGCA ACAAAAAGCC TTCGGCCATC TTCAAGCCAG 
TTGGCCGTCC CCATCTCCCT TCATGATATC CCACCACGGA TTGCTCAGGT CATAGAAAAT 
GAGGAGCAAT GGGACTTTGA CATTTTTGAG CTGGAAGCTG CAACCCAAAA TAGGCCTTTG 
ATTTATCTAG GTCTCAAAAC GTTTGCTCGT TTTGAAATCT GTGAGTTCTT ACAGTGCTCT 
GAGGCAACGC TGAAATCATG GTTTCAAATT ATTGAATCCA ATTACCATTC CTCTAACCCC 
TACCACAATT CCACGCACGC CGCGGATGTG CTTCACGCCA CTGCGTATTT CCTTTCCAGA 
AACAAGTTAA AGGACACTTT AGATCGCCTT GATGAGGTCG CTGCCCTCAT CGCTGCCACC 
ATCCACGATG TGGATCACCC CGGGAGAACC AACTCTTTCC TCTGCAATGC GGGAAAGGAG 
CTGGCCGTAC TGTACAATGA CACTGCTGTG CTAGAGAGCC ACCACGCAGC GCTGGCCTTC 
CAGCTGACCC TTGAAAATGA CAAATGCAAT ATCTTTAAAA ACACAGACAG GAATGACTAC 
CGGACACTAC GCCAGAGTAT CATTGACATG GTCCTAGCCA CAGAGATGAC AAAGCACTTT 
GAACACGTCA ACAAATTTAT CAACAGCATC AACAAGCCTC TGAAAGCACA GAAAAACGAG 
GTTCAGGAAA CTGGCAGAAG TCTGGAAGAT ATAAGAGCCA TGGTCAGGAC TCCAGAGAGC 
CGGACTCTCA TCAAAAGGAT GATGATTAAG TGTGCCGACG TGTCCAACCC CTGCCGGCCC 
CTCGAGCACT GCATCGAGTG GGCTGCACGC ATCTCAGAGG AGTATTTCTC CCAGACCGAT 
GAAGAGAAAC AGCTGGACTT GCCTGTGGTG ATGCCAGTGT TTGACAGAAA CACCTGCAGC 
ATCCCCAAGT CTCAGATCTC CTTCATCGAC TACTTCATCA CAGACATGTT TGATGCCTGG 
GATGCCTTTG TTTACCTGCC TAACCTTATG CGGCACCTGG AGGACAACTT CAAGTACTGG 
AAAGGACTGG ATGAGAAGGA GCTGTGCACC CTCCGGCCCC CTCCAGAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006229501.1
CDS198..2468
Translation

Target ORF information:

RefSeq Version XM_006229439.3
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus phosphodiesterase 8A (Pde8a), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006229439.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGGGCTGCG CCCCGAGCAT CCACATTTCC GACAACCGAG TGTCGAACCC CAGCGACAGC 
GACGGCGAGG ACGAGGACGT CCGGGTCCCC GCGCTGAGGT CTAGACAGCG ATGGTCGACC 
CCGCCCGGGC TCGTGGAGCC TCAGCCTCGC GACAGCGGTG CCTCGAAGGT ATCAGTTGCT 
GATGTGCAAT TTGGCCCGAT GAGATTTCAT CAAGACCAGC TTCAGGTACT TTTAGTGTTT 
ACCAAAGAAG ATAGCCAGTG TAATGGATTC TACAGGGCAT GTGAAAAAGC CGGGTTTAAG 
TGCACAGTCA CCAAGGAGGT GCAGACTGTC CTTGCCTGTT TCCAGGACAA ACTCCATGAC 
ATCATCATCA TAGACCACAG AAATCCCCGA CAGATGGATG CGGAGGCAAT GTGCAGGGCT 
TGTAACTCAG TGTTCACTGC GTTAGAGAAG AGTCAAGAAG CGATTGAGAT CACAAGTGAG 
GACCACATCA TCCAGTATGC AAACCCTGCA TTTGAAACAA CAATGGGTTA TCAGTCGGGA 
GAACTAATAG GGAAGGAGTT AGCACAAGTG CCCATCAATG AGAAGAAAGG TGACTTGCTG 
GATGCCATAA ATTCCTGTGT CACAATCGAC AAGGAGTGGC AAGGAGTTTA CCATACCCAA 
AAGAAAAATG GAGATAACAT CCAACAAAAT GTGAAGATAA TACCTGTCAT TGGGCAGGGA 
GGAAAAATCA GACACTATGT GTCCATTATC AGAGTGTGCA ATGGCAGCAA TAAGGTTGAG 
ACCACAACTG AGTGTGTTCA GACTGATAGT CAAACAGATA GTCAGGCAGG CAAGCATAAA 
GACAGGAGGA AACATTCGAT GGACGTCAAA GCGGTTTCCT CCAGAACAAG CGAAGTTTCC 
AATCAGAGAA GACATTCCTC TCTGGCCCGG ATACATTCTA TGATGATTGA GGCACCAATC 
ACCAAGGTAA TCAATATTCT TAGTGCTGCC CAGGAAAACA GTCCTGTGTC TGTGACAGAA 
GCCTTGAACC ATGTGCTGGA TATCCTGAGA ACCACTGAAC TGTATTCACC CCAGTTTAAT 
ACTGAGGATG ACCCTCACGC CACTGACCTC GTTGGGGGCT TAATGTCTGA TGGCTTACGG 
AGGTTTTCAG GAAATGAGTA TACTCTTGCA ACAAAAAGCC TTCGGCCATC TTCAAGCCAG 
TTGGCCGTCC CCATCTCCCT TCATGATATC CCACCACGGA TTGCTCAGGT CATAGAAAAT 
GAGGAGCAAT GGGACTTTGA CATTTTTGAG CTGGAAGCTG CAACCCAAAA TAGGCCTTTG 
ATTTATCTAG GTCTCAAAAC GTTTGCTCGT TTTGAAATCT GTGAGTTCTT ACAGTGCTCT 
GAGGCAACGC TGAAATCATG GTTTCAAATT ATTGAATCCA ATTACCATTC CTCTAACCCC 
TACCACAATT CCACGCACGC CGCGGATGTG CTTCACGCCA CTGCGTATTT CCTTTCCAGA 
AACAAGTTAA AGGACACTTT AGATCGCCTT GATGAGGTCG CTGCCCTCAT CGCTGCCACC 
ATCCACGATG TGGATCACCC CGGGAGAACC AACTCTTTCC TCTGCAATGC GGGAAAGGAG 
CTGGCCGTAC TGTACAATGA CACTGCTGTG CTAGAGAGCC ACCACGCAGC GCTGGCCTTC 
CAGCTGACCC TTGAAAATGA CAAATGCAAT ATCTTTAAAA ACACAGACAG GAATGACTAC 
CGGACACTAC GCCAGAGTAT CATTGACATG GTCCTAGCCA CAGAGATGAC AAAGCACTTT 
GAACACGTCA ACAAATTTAT CAACAGCATC AACAAGCCTC TGAAAGCACA GAAAAACGAG 
GTTCAGGAAA CTGGCAGAAG TCTGGAAGAT ATAAGAGCCA TGGTCAGGAC TCCAGAGAGC 
CGGACTCTCA TCAAAAGGAT GATGATTAAG TGTGCCGACG TGTCCAACCC CTGCCGGCCC 
CTCGAGCACT GCATCGAGTG GGCTGCACGC ATCTCAGAGG AGTATTTCTC CCAGACCGAT 
GAAGAGAAAC AGCTGGACTT GCCTGTGGTG ATGCCAGTGT TTGACAGAAA CACCTGCAGC 
ATCCCCAAGT CTCAGATCTC CTTCATCGAC TACTTCATCA CAGACATGTT TGATGCCTGG 
GATGCCTTTG TTTACCTGCC TAACCTTATG CGGCACCTGG AGGACAACTT CAAGTACTGG 
AAAGGACTGG ATGAGAAGGA GCTGTGCACC CTCCGGCCCC CTCCAGAATA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Phosphodiesterase-8A binds to and regulates Raf-1 kinase.
Proceedings of the National Academy of Sciences of the United States of America110(16)E1533-42(2013 Apr)
Brown KM,Day JP,Huston E,Zimmermann B,Hampel K,Christian F,Romano D,Terhzaz S,Lee LC,Willis MJ,Morton DB,Beavo JA,Shimizu-Albergine M,Davies SA,Kolch W,Houslay MD,Baillie GS


book

Distribution of PDE8A in the nervous system of the Sprague-Dawley rat.
Journal of chemical neuroanatomy42(3)184-91(2011 Nov)
Kruse LS,M?ller M,Kruuse C


book

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA


book

Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signaling.
Annual review of biochemistry76481-511(2007)
Conti M,Beavo J


book

Molecular comparison of rat cyclic nucleotide phosphodiesterase 8 family: unique expression of PDE8B in rat brain.
Gene31921-31(2003 Nov)
Kobayashi T,Gamanuma M,Sasaki T,Yamashita Y,Yuasa K,Kotera J,Omori K