LOC580217 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC580217
Entrez Gene ID	580217
Full Name	protein FAM179B
Gene Type	protein-coding
Organism	Strongylocentrotus purpuratus(purple sea urchin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_780290.4	XP_785383.4	protein FAM179B
XM_030999675.1	XP_030855535.1	TOG array regulator of axonemal microtubules protein 1 isoform X1
XM_030999676.1	XP_030855536.1	TOG array regulator of axonemal microtubules protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC580217 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC580217 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSd23064	XM_780290.4 Latest version!	Strongylocentrotus purpuratus protein FAM179B (LOC580217), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OSd65612	XM_030999675.1 Latest version!	Strongylocentrotus purpuratus TOG array regulator of axonemal microtubules protein 1 (LOC580217), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSd65613	XM_030999676.1 Latest version!	Strongylocentrotus purpuratus TOG array regulator of axonemal microtubules protein 1 (LOC580217), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC580217 ( XM_780290.4 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_785383.4 Strongylocentrotus purpuratus protein FAM179B (LOC580217), mRNA.

Price & Availability↑

CloneID	OSd23064
Clone ID Related Accession (Same CDS sequence)	XM_780290.4
Accession Version	XM_780290.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-23
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	protein FAM179B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011993022.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 24, 2015 this sequence version replaced XM_780290.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Strongylocentrotus purpuratus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGGA AAGAAAGCCA TGAAGACGGT GCCTTACCTG AGGTTATTGG TCTTCAGGTT 
CGGCATGATG AGGACAGTCC GGAAGAAAAT GACTTGTCTA TGTCCCTCTC TAAAGCAACA 
ATGGACAGGA TGGCAAGGAA ACAGATAGAG AAGAAGGAGG AGTTGGAGCA GCGCAAGGCG 
GATCGTAAGG CCGAGAAGGA GCGTGCTGAT GCAGAGAGAC GCACTCAGGA GAAGGAGCGC 
GAGGTACAGA GAAGAGTTCT GGAGAAGGAA ATAGATGCCC AGAGAAAAGT GATGCAGGAA 
CGTGAAAGGG AGCAGCAGCG GCTTGATTTC GAGAAGCAAC AGAGGGAGAA GGAAAAGTTC 
AAGGAGGAGA GAGAGCGCCA GAGGGAGGAG AGGATGAGAG AGAGGGAGGA ACAGAAGAGA 
GAGCGAGAAG AAAGACTGCG GGAGGACAGA GAGCGTCAGA GAGCAGAGCG GGAAGAGAGG 
GAGCATCAGA AGGTAGAGAG AGAGAGGATA AGGGAGGAGC AACAAGGCAA GGCTAGGGAG 
AGACAGAGGG AGAAACTCAA GCAGCTTGAG TTAGAGGGTG CCATGGATCA TGATGATGGG 
GGTGATCTTG CTATCAATGG AGCTATGCTA GATAGCAGTA GACCTAATCC TCCAGCCAGA 
GTAACGGCCA AGCCTCCTGC AGCTGCTACA CCACCTAAGA AGAAAAACAG TGGGATTTCT 
GTGACAAAGA ATTCTAGTGG TCCTCCTGCT GTAACATCAC CACCAGGGAA CGACGTCCAG 
AGCAGAGAGA TGAAACCAGT TAATAATGTA GATGTAGCTG TCAAAGATTG TTTTAGATTG 
ATCGGACATG AAGACTGGGA AATCAAGTTG GATGGCTTGT TCATTATTCA AAGGCTAGCC 
ATTTTCCATA GCAATGACTT AGGAGGTCAG CTGCATGCAA TGGTCGTGGC TGTTCTTAAT 
GAGGTCAAGA ATCTGAGGTC AACGGTTGCT AGGGCTGCGT TATCTACACT AGGAGATATG 
TTCACGTCAT TTAAAACCAG TATGGACAAG GACTTGGATC CGATCTGTCG TATCTTGTTA 
CCCAAGGCTG GTGAGAGCAA TGCTTTCATT CGAGAGGATG TTGACAAGGC CTTAGATGCC 
ATGGTGAAGA ACGCAAACCC ACAAAGAGTA TTAGGAGCGT TGATCACTGC TGGAGCAAGT 
CATAAGAGTG CTATGGTACG CAAGACGACT AGTGTGTTTC TGGATGCAGT GGTAGAGCGT 
ATGGGACCAG GAAGGATACT GAGTGGAGTG AAGGATGTCA CTGATAAGAT CTTACCTGTA 
ACAGCTCAGC TAGCACTCGA CAATGGACAA GAAACAAGGT ACTATGCGAG GAAGATGTTC 
TTTAATCTGA TGCACCATGA GGACTTTGAT CGGTTGATTG AGAAACATGT CCCAGCTAAA 
AATCTCAAGA GGATCAGAGA CATCCTGGAA AACCTCAAGA ATAAGGGTCT TGGTGATGTA 
CCCTCAGATA CTTCATCTGC CAAAGCTCGT CGTAGCAATC CTGGTAGTCG AGCCAACTCC 
GGATCTCAAT CATATTCTAG GGATAGGAAT GAATACAGTG AGGTGGACGG CACCCCACCA 
AGCTCAGTCA AGAAAGGAAA GCGGTCTGGC GGAGGAGCCC GTGGAGAAGA GAACGAGACT 
ATCAAGAGTC TTTGTGAAGG TCTAGGAGCA TCGGATTGGA TGTCTAGGTT ACAAGCCATT 
GAGAGACTAC AATCCATGTG TGAAACAAAT CAAGACTTGG TTGATGGGAG CTTAGTCAAG 
ATCTTTGATA AATTCATCTC GAGGTTATCA GACTCGAACA GTAAAGTGAA CATAGCTGCT 
CTGACAACCA TGAAAGACAT CGTACCTCGG CTTGGTGAAT CCTTACCGGC TGTCGTCAAT 
AACCTCGTCC CAATCCTAGT CCAGAACCTT GCAGCAAAGA ACCCTAGCAT CAGTCAGACC 
AGTAATGACA TCCTAGATCT CATTCTGGAA CATGTTGATA TGCTGGTATT AGTTCAGCCT 
TACTCCAATG CTACTCAGTA TGGCAACGTG AGAGCCAGGC CAGCAATGGT AGAAAAGTTG 
TCCTACCTTG TGACCAAGGT GTACCCACGG AAGCAACAGA CGGTCGTCAG GAACGTTCTA 
CCTGTTCTAT GGTACCTCCT GGGCAACATG ACAGGTAGCG GGGCGGTACC GGGGGGTTCA 
GGCAACCTAC GTAGTGCCAC AGCCGTCCTT GCTAGTAGCC TTCATGATCA GATGGGTGAA 
GGCTTACTCC AACATGCTCA GAACCTACCC CCTAGGAGTG TCAAGACACT CAAAGAACTC 
ATCGATGAAG AGACTACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_785383.4
CDS	59..2419
Translation	MRRKESHEDGALPEVIGLQVRHDEDSPEENDLSMSLSKATMDRMARKQIEKKEELEQRKADRKAEKERADAERRTQEKEREVQRRVLEKEIDAQRKVMQEREREQQRLDFEKQQREKEKFKEERERQREERMREREEQKREREERLREDRERQRAEREEREHQKVERERIREEQQGKARERQREKLKQLELEGAMDHDDGGDLAINGAMLDSSRPNPPARVTAKPPAAATPPKKKNSGISVTKNSSGPPAVTSPPGNDVQSREMKPVNNVDVAVKDCFRLIGHEDWEIKLDGLFIIQRLAIFHSNDLGGQLHAMVVAVLNEVKNLRSTVARAALSTLGDMFTSFKTSMDKDLDPICRILLPKAGESNAFIREDVDKALDAMVKNANPQRVLGALITAGASHKSAMVRKTTSVFLDAVVERMGPGRILSGVKDVTDKILPVTAQLALDNGQETRYYARKMFFNLMHHEDFDRLIEKHVPAKNLKRIRDILENLKNKGLGDVPSDTSSAKARRSNPGSRANSGSQSYSRDRNEYSEVDGTPPSSVKKGKRSGGGARGEENETIKSLCEGLGASDWMSRLQAIERLQSMCETNQDLVDGSLVKIFDKFISRLSDSNSKVNIAALTTMKDIVPRLGESLPAVVNNLVPILVQNLAAKNPSISQTSNDILDLILEHVDMLVLVQPYSNATQYGNVRARPAMVEKLSYLVTKVYPRKQQTVVRNVLPVLWYLLGNMTGSGAVPGGSGNLRSATAVLASSLHDQMGEGLLQHAQNLPPRSVKTLKELIDEETT

Target ORF information:

RefSeq Version	XM_780290.4
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus protein FAM179B (LOC580217), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_780290.4

ORF Insert Sequence:

ATGAGGAGGA AAGAAAGCCA TGAAGACGGT GCCTTACCTG AGGTTATTGG TCTTCAGGTT 
CGGCATGATG AGGACAGTCC GGAAGAAAAT GACTTGTCTA TGTCCCTCTC TAAAGCAACA 
ATGGACAGGA TGGCAAGGAA ACAGATAGAG AAGAAGGAGG AGTTGGAGCA GCGCAAGGCG 
GATCGTAAGG CCGAGAAGGA GCGTGCTGAT GCAGAGAGAC GCACTCAGGA GAAGGAGCGC 
GAGGTACAGA GAAGAGTTCT GGAGAAGGAA ATAGATGCCC AGAGAAAAGT GATGCAGGAA 
CGTGAAAGGG AGCAGCAGCG GCTTGATTTC GAGAAGCAAC AGAGGGAGAA GGAAAAGTTC 
AAGGAGGAGA GAGAGCGCCA GAGGGAGGAG AGGATGAGAG AGAGGGAGGA ACAGAAGAGA 
GAGCGAGAAG AAAGACTGCG GGAGGACAGA GAGCGTCAGA GAGCAGAGCG GGAAGAGAGG 
GAGCATCAGA AGGTAGAGAG AGAGAGGATA AGGGAGGAGC AACAAGGCAA GGCTAGGGAG 
AGACAGAGGG AGAAACTCAA GCAGCTTGAG TTAGAGGGTG CCATGGATCA TGATGATGGG 
GGTGATCTTG CTATCAATGG AGCTATGCTA GATAGCAGTA GACCTAATCC TCCAGCCAGA 
GTAACGGCCA AGCCTCCTGC AGCTGCTACA CCACCTAAGA AGAAAAACAG TGGGATTTCT 
GTGACAAAGA ATTCTAGTGG TCCTCCTGCT GTAACATCAC CACCAGGGAA CGACGTCCAG 
AGCAGAGAGA TGAAACCAGT TAATAATGTA GATGTAGCTG TCAAAGATTG TTTTAGATTG 
ATCGGACATG AAGACTGGGA AATCAAGTTG GATGGCTTGT TCATTATTCA AAGGCTAGCC 
ATTTTCCATA GCAATGACTT AGGAGGTCAG CTGCATGCAA TGGTCGTGGC TGTTCTTAAT 
GAGGTCAAGA ATCTGAGGTC AACGGTTGCT AGGGCTGCGT TATCTACACT AGGAGATATG 
TTCACGTCAT TTAAAACCAG TATGGACAAG GACTTGGATC CGATCTGTCG TATCTTGTTA 
CCCAAGGCTG GTGAGAGCAA TGCTTTCATT CGAGAGGATG TTGACAAGGC CTTAGATGCC 
ATGGTGAAGA ACGCAAACCC ACAAAGAGTA TTAGGAGCGT TGATCACTGC TGGAGCAAGT 
CATAAGAGTG CTATGGTACG CAAGACGACT AGTGTGTTTC TGGATGCAGT GGTAGAGCGT 
ATGGGACCAG GAAGGATACT GAGTGGAGTG AAGGATGTCA CTGATAAGAT CTTACCTGTA 
ACAGCTCAGC TAGCACTCGA CAATGGACAA GAAACAAGGT ACTATGCGAG GAAGATGTTC 
TTTAATCTGA TGCACCATGA GGACTTTGAT CGGTTGATTG AGAAACATGT CCCAGCTAAA 
AATCTCAAGA GGATCAGAGA CATCCTGGAA AACCTCAAGA ATAAGGGTCT TGGTGATGTA 
CCCTCAGATA CTTCATCTGC CAAAGCTCGT CGTAGCAATC CTGGTAGTCG AGCCAACTCC 
GGATCTCAAT CATATTCTAG GGATAGGAAT GAATACAGTG AGGTGGACGG CACCCCACCA 
AGCTCAGTCA AGAAAGGAAA GCGGTCTGGC GGAGGAGCCC GTGGAGAAGA GAACGAGACT 
ATCAAGAGTC TTTGTGAAGG TCTAGGAGCA TCGGATTGGA TGTCTAGGTT ACAAGCCATT 
GAGAGACTAC AATCCATGTG TGAAACAAAT CAAGACTTGG TTGATGGGAG CTTAGTCAAG 
ATCTTTGATA AATTCATCTC GAGGTTATCA GACTCGAACA GTAAAGTGAA CATAGCTGCT 
CTGACAACCA TGAAAGACAT CGTACCTCGG CTTGGTGAAT CCTTACCGGC TGTCGTCAAT 
AACCTCGTCC CAATCCTAGT CCAGAACCTT GCAGCAAAGA ACCCTAGCAT CAGTCAGACC 
AGTAATGACA TCCTAGATCT CATTCTGGAA CATGTTGATA TGCTGGTATT AGTTCAGCCT 
TACTCCAATG CTACTCAGTA TGGCAACGTG AGAGCCAGGC CAGCAATGGT AGAAAAGTTG 
TCCTACCTTG TGACCAAGGT GTACCCACGG AAGCAACAGA CGGTCGTCAG GAACGTTCTA 
CCTGTTCTAT GGTACCTCCT GGGCAACATG ACAGGTAGCG GGGCGGTACC GGGGGGTTCA 
GGCAACCTAC GTAGTGCCAC AGCCGTCCTT GCTAGTAGCC TTCATGATCA GATGGGTGAA 
GGCTTACTCC AACATGCTCA GAACCTACCC CCTAGGAGTG TCAAGACACT CAAAGAACTC 
ATCGATGAAG AGACTACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC580217 ( XM_030999675.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030855535.1 Strongylocentrotus purpuratus TOG array regulator of axonemal microtubules protein 1 (LOC580217), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSd65612
Clone ID Related Accession (Same CDS sequence)	XM_030999675.1
Accession Version	XM_030999675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	TOG array regulator of axonemal microtubules protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAA CGGTTGCCAA GTTGCCTGTA TACAACTTCG GGCAAGGTGA GCACCGCACG 
GTAATGAATG GGAGCGATGC GGACGAGTCC GGTGAGCGTG ATGAGCGAGA AATGGATGAT 
GATGATGACC TTTTCAGACA GTTGCAAGAC AGCAGTTATG TCCAGCATAG GGAATATATT 
CTTGATGAAC TTGTTTCTAA AGTCAGGAGG AATGGAGGTA AAGCTCCATT TAAAAATCCT 
CGTACACTTT TCAAGGGTCT TGCACTTGTT ATGCGAGACC AGGAAAAGAA TGTGCGACTC 
AAGTGCATTC AATTTATCAT GGACTTGGTT CCAATTCTCA AGCAGGAGCT TGATGCCTGC 
ATGATGCTCA TCATGCTTGA TCTGATATCA AGCTTTAGTG ATGCAAAGGT ATCAATACGG 
AAGGCTGCTG TTCAGACGCT GCATATGTAC ATGAAGCATA CAACTAACAT TGAGCAAATC 
TTCAACTCAA TTGTGAAGTA TGGGTTAGAA AATGAAGACC CCAAAATCAG AGCCGAAACA 
GCAGTTTCAA TTCCTGTTCT TCTGACAGGG GAATTTGCAA ACACTGACTT GTCAATTGTT 
ACTCGTGCAT TGGCTAGAAA GTTCACCGAT GATACTGATT TGATGGTAAC TGCAGGCGTA 
TCTTTGGACA GAATCTGTTC TTTGGTTGGG GAAGCATCAT TTAACTCGTA CATAGACAGC 
TTGCCAGCAA GAATCAAAAG TGGTTATTGC AAAAGAACAG GGAGACCTGA TCCTAGCCCA 
AGAATCCCCT CAGAAGGATA TTCAACCTCA GGATCTCTAG ATTGGAATTC CAACCGAACA 
ACGGAACCTA AAACTGTCCC AGGTGACGAG CGTAGATCGA GGCCTAAGCC AAATGTTGAA 
AAACAGTTTG GAATTGTACC ACAAGATGTG CTTGATCAAT TGTCAGACCA AGAACACTGG 
ACTGAGAGAG CATCTGGGGT ACAGGAGCTC AAAGAAATAG TTGTGGAACT CAAGGACATA 
TCACAGCTTA CACCTCACAT GATCAATTTC ATCAGTTTCC TGTGCAACTT ACTTGATGAC 
TCCAATTTCA AAGTTAGCTT GCACACCTTG GAAATATTTC AAGAACTGGT TCTCAAGTTC 
AACCAAAACA TTAAACCATT CGTTCGACAC ATAGTAAATG CTCTTTCAAA GCGCTTAGGA 
GACAACAAGA CAGTTATTAG ACAAGCAAAC ATGAAGGTGA TGATGCAGCT TATGCAAAGT 
GTAGGACCCA AACCTGTAGT TCATGTAGTA TCAGAGTTGA AGAACCAGCG GAAATCTGGA 
GTACGAGAGG AAGCTTTGAA CATTGTGATT GCAGCTCTTC TGACATTTCC TAGTTATGAC 
TTTGACCTGC CAGTCATTTG CCGAACACTG TCACAATGTC TAAATGATCA GAAGAGAAGG 
GTAAGGCAAG CAACGCTAGA ACTGTTTGCT GCCCTGGCTC AAGCAATGGG ACCAACTAGA 
ATAGGTCCTC TCATTGATGC TGTAGATCAG ATTGAAATGA AAACAGAAGA AGAAGGAGTC 
ATGAAGGCAG TTCAAGCAAG ACTGGCAAGG AGACAGCTAC CCAGATTAAA TGAGAATGGT 
TTAGTGGAGT ATGCATCTCA GACACCATCG TCAGCAGCAG CTGCAGGCAG AACAGCATCA 
TCAGCTGGTT ATGATACTGA ATGGATTTTG AAAGCTACAA GAAACTCAAG TGCAAAAGAA 
AGAGCCTTCA CTGATCCTGG GATAGAGCTT TCCCAACCAT CACCTACAAG AGCTAAACCA 
CCCATGAGTA ATGCTGCCAA CCCTGATTCA CAACCTCCTA AAAGGTTTCT GAGTGCAGGG 
AGAGGTCGCT CCAAACTACC ATGGGAAGAA GTGAGAGATG CAAGGGCTGG AGAGGATAAG 
GAAGATGGAG CTGGACAGAA GACCACAGGC AAAGGCAGAG TGCAAATAAA CAGTGCTCCA 
ACTCAGAAAC GTGAGGAGCC TGGGATAAGA CGACCGCCTA TCAAAGCTCG AAATACATGG 
AACGGAGGGG AAGATGTGTG GGACAATGCT AGACGTAGTC ACAACAACCT TACTGATATG 
GGATCTGACC AAGGTTCAGG TTCAGCTTCT AGTGGATCGT ACCGTCAGAT CCATCTTGAC 
AAACTCAAGA GAGCATCTCT AAATACCTAC CCTGGGAGGG GGCGAGGTAG CTGGGAGGAT 
GAGCCTCACG CATCAGGAAG CCACAGTGCA CCAGTCACAG AGAGAGACCT GAATCTTTGG 
AAAACTTTTT CTTTTGATGG AGATAATATA TTTGGTGGAC CAGATCCATT CATTGGCAAC 
ACAGCAGTCA TCCCCAAGAA GCGTGGCTCC CCCAAACAGC ACCCCTCCCA GCAGCATCAC 
CATCATCAGG AGGACTCTGA GCACCTCGCC CACCCCGCCC TCAGGCAACA GCTATCCTAC 
AGTGAGAAGT ACATGCCGTC TGTGGGAGCA TCCCGCTCCA GGAATGAGGA GGAAAGGGAG 
CAGCGAATGA ATCGGAAGAG CCTGTCAAAC TCCTGGCCTG ATCAGGGTAT CGGCATGGAT 
GATGGTCCAT CCTCGTCCTC CAATGCTGGG CGTAAGAAGA TGGACCTTAT GTTGGATTCT 
CTCCATGACT TCCATGCAAT TTCTCAATAT GACGTCCCTT CACCCAACAA ATCCAATTTC 
CCTGCCGGTA CCTCCCCCAT CTCACCCATC CCCCTGAAGG CTACACTGGC CCGATCAGCA 
GGGAGGAGGG GGCAAGGTAG AGCACCGTTG GTACCCCTAG AGAAGACTAC TAGTCCTCGG 
GATGATTATG ATGACTGGGA TTCTTATCAG GATACCCACC GATCGGATTG GACAGACACT 
GACGTTGTTC CTAATAGATC CTCACTGGAT AACATAAGAG GCAGCGCAGC CAAGAAGCGA 
GCAGAGAAAA TGGCACGGGG GCAGGATAAA TCCTCCGGTG GGAGCAACAG GGACTTAGAC 
GATAGTGGGT TATTCAGTCT ACAGTCTACG GGGCGCTTGG ATTTCGATGA TGGGACGCAT 
AATGGAGCTC AAACTACATA CGTCAAGAAG AAAAATCAAA CTGGAAAGAA AGGATCTGAC 
TCTCCATTCA GTTCTAAGCC ACGTATAGCA CGGTCAAACT CTGGGAAAGG TTCAAGACGA 
ACTGCTGCCG GGGAGCAACG CCAAGATGGC GGTAGCTCTT CAGCAAACCT GGAGTTTAAT 
CCCTCGGGAG GAGTGACGTT CAGAGACAAT GCGACGTCTG ACGTGAGCAT CATCGGTCAT 
GGCTTCGGGA ATGGACGGGG CATCGGTCAT AAGGACATTG ATCTCTCAGA TAATCATGTG 
CCTGCAGGCA AAGGACAGAA TTCTCGGGAT AGGAAGAGAA CAAAGGGGTC TATCCTGTCT 
CCTCTATCGA TGGCGTCTTT ACAGCATCCT GGAGGAGTTG ATCTGGATGA CAACGGGGCT 
TTGGTTGGGG AGGACTTTGC CATGGTTATT GGAAAAGGTG TGTTTGGTAA TGATGCTAAT 
ATAGTAGAGT ACCCACGATC AAATGGTGGA GAGAAGACAA TGAGGAGGAA AGAAAGCCAT 
GAAGACGGTG CCTTACCTGA GGTTATTGGT CTTCAGGTTC GGCATGATGA GGACAGTCCG 
GAAGAAAATG ACTTGTCTAT GTCCCTCTCT AAAGCAACAA TGGACAGGAT GGCAAGGAAA 
CAGATAGAGA AGAAGGAGGA GTTGGAGCAG CGCAAGGCGG ATCGTAAGGC CGAGAAGGAG 
CGTGCTGATG CAGAGAGACG CACTCAGGAG AAGGAGCGCG AGGTACAGAG AAGAGTTCTG 
GAGAAGGAAA TAGATGCCCA GAGAAAAGTG ATGCAGGAAC GTGAAAGGGA GCAGCAGCGG 
CTTGATTTCG AGAAGCAACA GAGGGAGAAG GAAAAGTTCA AGGAGGAGAG AGAGCGCCAG 
AGGGAGGAGA GGATGAGAGA GAGGGAGGAA CAGAAGAGAG AGCGAGAAGA AAGACTGCGG 
GAGGACAGAG AGCGTCAGAG AGCAGAGCGG GAAGAGAGGG AGCATCAGAA GGTAGAGAGA 
GAGAGGATAA GGGAGGAGCA ACAAGGCAAG GCTAGGGAGA GACAGAGGGA GAAACTCAAG 
CAGCTTGAGT TAGAGGGTGC CATGGATCAT GATGATGGGG GTGATCTTGC TATCAATGGA 
GCTATGCTAG ATAGCAGTAG ACCTAATCCT CCAGCCAGAG TAACGGCCAA GCCTCCTGCA 
GCTGCTACAC CACCTAAGAA GAAAAACAGT GGGATTTCTG TGACAAAGAA TTCTAGTGGT 
CCTCCTGCTG TAACATCACC ACCAGGGAAC GACGTCCAGA GCAGAGAGAT GAAACCAGTT 
AATAATGTAG ATGTAGCTGT CAAAGATTGT TTTAGATTGA TCGGACATGA AGACTGGGAA 
ATCAAGTTGG ATGGCTTGTT CATTATTCAA AGGCTAGCCA TTTTCCATAG CAATGACTTA 
GGAGGTCAGC TGCATGCAAT GGTCGTGGCT GTTCTTAATG AGGTCAAGAA TCTGAGGTCA 
ACGGTTGCTA GGGCTGCGTT ATCTACACTA GGAGATATGT TCACGTCATT TAAAACCAGT 
ATGGACAAGG ACTTGGATCC GATCTGTCGT ATCTTGTTAC CCAAGGCTGG TGAGAGCAAT 
GCTTTCATTC GAGAGGATGT TGACAAGGCC TTAGATGCCA TGGTCAAGAA CGCAAACCCA 
CAAAGAGTAT TAGGAGCATT GATCACTGCT GGAGCAAGTC ATAAGAGTGC TATGGTACGC 
AAGACGACCA GTGTGTTTCT GGATGCAGTG GTAGAGCGTA TGGGACCAGG AAGGATACTG 
AGTGGAGTGA AGGATGTCAC TGATAAGATC TTACCTGTTA CAGCTCAGCT AGCGCTCGAC 
AATGGACAAG AAACAAGGTA CTATGCGAGG AAGATGTTCT TTAATCTGAT GCACCATGAG 
GACTTTGATC GGTTGATTGA GAAACATGTC CCAGCTAAGA ATCTCAAGAG GATCAGAGAC 
ATCCTGGAAA ACCTCAAGAA TAAGGGTCTT GGTGATGTCC CATCAGATAC TTCATCTGCC 
AAAGCTCGTC GTAGCAATCC TGGTAGTCGA GCCAACTCTG GATCTCAATC ATATTCTAGG 
GATAGAAATG AATACAGTGA GGTGGACGGC ACCCCACCAA GCTCAGTCAA GAAAGGAAAG 
CGGTCTGGCG GAGGAGCCCG TGGAGAAGAG AACGAGACTA TCAAGAGTCT TTGTGAAGGT 
CTAGGAGCAT CGGATTGGAT GTCTAGGTTA CAAGCCATTG AGAGACTACA ATCCATGTGT 
GAAACAAATC AAGACTTGGT TGATGGGAGC TTAGTCAAGA TCTTTGATAA ATTCATCTCG 
AGGTTATCAG ACTCGAACAG TAAAGTGAAC ATAGCTGCTC TGACAACCAT GAAAGACATC 
GTACCTCGGC TTGGTGAATC CTTACCGGCT GTCGTCAATA ACCTCGTCCC AATCCTAGTC 
CAGAACCTTG CAGCAAAGAA CCCTAGCATC AGTCAGACCA GTAATGACAT CCTAGATCTC 
ATTCTGGAAC ATGTTGATAT GCTGGTATTA GTTCAGCCTT ACTCCAATGC TACTCAGTAT 
GGCAACGTGA GAGCCAGGCC AGCAATGGTA GAAAAGTTGT CCTACCTTGT GACCAAGGTG 
TACCCACGGA AGCAACAGAC GGTCGTCAGG AACGTTCTAC CTGTTCTATG GTACCTCCTG 
GGCAACATGA CAGGTAGCGG GGCGGTACCG GGGGGTTCAG GCAACCTACG TAGTGCCACA 
GCCGTCCTTG CTAGTAGCCT TCATGATCAG ATGGGTGAAG GCTTACTCCA ACATGCTCAG 
AACCTACCCC CTAGGAGTGT CAAGACACTC AAAGAACTCA TCGATGAAGA GACTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030855535.1
CDS	226..6165
Translation	MAGTVAKLPVYNFGQGEHRTVMNGSDADESGERDEREMDDDDDLFRQLQDSSYVQHREYILDELVSKVRRNGGKAPFKNPRTLFKGLALVMRDQEKNVRLKCIQFIMDLVPILKQELDACMMLIMLDLISSFSDAKVSIRKAAVQTLHMYMKHTTNIEQIFNSIVKYGLENEDPKIRAETAVSIPVLLTGEFANTDLSIVTRALARKFTDDTDLMVTAGVSLDRICSLVGEASFNSYIDSLPARIKSGYCKRTGRPDPSPRIPSEGYSTSGSLDWNSNRTTEPKTVPGDERRSRPKPNVEKQFGIVPQDVLDQLSDQEHWTERASGVQELKEIVVELKDISQLTPHMINFISFLCNLLDDSNFKVSLHTLEIFQELVLKFNQNIKPFVRHIVNALSKRLGDNKTVIRQANMKVMMQLMQSVGPKPVVHVVSELKNQRKSGVREEALNIVIAALLTFPSYDFDLPVICRTLSQCLNDQKRRVRQATLELFAALAQAMGPTRIGPLIDAVDQIEMKTEEEGVMKAVQARLARRQLPRLNENGLVEYASQTPSSAAAAGRTASSAGYDTEWILKATRNSSAKERAFTDPGIELSQPSPTRAKPPMSNAANPDSQPPKRFLSAGRGRSKLPWEEVRDARAGEDKEDGAGQKTTGKGRVQINSAPTQKREEPGIRRPPIKARNTWNGGEDVWDNARRSHNNLTDMGSDQGSGSASSGSYRQIHLDKLKRASLNTYPGRGRGSWEDEPHASGSHSAPVTERDLNLWKTFSFDGDNIFGGPDPFIGNTAVIPKKRGSPKQHPSQQHHHHQEDSEHLAHPALRQQLSYSEKYMPSVGASRSRNEEEREQRMNRKSLSNSWPDQGIGMDDGPSSSSNAGRKKMDLMLDSLHDFHAISQYDVPSPNKSNFPAGTSPISPIPLKATLARSAGRRGQGRAPLVPLEKTTSPRDDYDDWDSYQDTHRSDWTDTDVVPNRSSLDNIRGSAAKKRAEKMARGQDKSSGGSNRDLDDSGLFSLQSTGRLDFDDGTHNGAQTTYVKKKNQTGKKGSDSPFSSKPRIARSNSGKGSRRTAAGEQRQDGGSSSANLEFNPSGGVTFRDNATSDVSIIGHGFGNGRGIGHKDIDLSDNHVPAGKGQNSRDRKRTKGSILSPLSMASLQHPGGVDLDDNGALVGEDFAMVIGKGVFGNDANIVEYPRSNGGEKTMRRKESHEDGALPEVIGLQVRHDEDSPEENDLSMSLSKATMDRMARKQIEKKEELEQRKADRKAEKERADAERRTQEKEREVQRRVLEKEIDAQRKVMQEREREQQRLDFEKQQREKEKFKEERERQREERMREREEQKREREERLREDRERQRAEREEREHQKVERERIREEQQGKARERQREKLKQLELEGAMDHDDGGDLAINGAMLDSSRPNPPARVTAKPPAAATPPKKKNSGISVTKNSSGPPAVTSPPGNDVQSREMKPVNNVDVAVKDCFRLIGHEDWEIKLDGLFIIQRLAIFHSNDLGGQLHAMVVAVLNEVKNLRSTVARAALSTLGDMFTSFKTSMDKDLDPICRILLPKAGESNAFIREDVDKALDAMVKNANPQRVLGALITAGASHKSAMVRKTTSVFLDAVVERMGPGRILSGVKDVTDKILPVTAQLALDNGQETRYYARKMFFNLMHHEDFDRLIEKHVPAKNLKRIRDILENLKNKGLGDVPSDTSSAKARRSNPGSRANSGSQSYSRDRNEYSEVDGTPPSSVKKGKRSGGGARGEENETIKSLCEGLGASDWMSRLQAIERLQSMCETNQDLVDGSLVKIFDKFISRLSDSNSKVNIAALTTMKDIVPRLGESLPAVVNNLVPILVQNLAAKNPSISQTSNDILDLILEHVDMLVLVQPYSNATQYGNVRARPAMVEKLSYLVTKVYPRKQQTVVRNVLPVLWYLLGNMTGSGAVPGGSGNLRSATAVLASSLHDQMGEGLLQHAQNLPPRSVKTLKELIDEETT

Target ORF information:

RefSeq Version	XM_030999675.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus TOG array regulator of axonemal microtubules protein 1 (LOC580217), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030999675.1

ORF Insert Sequence:

ATGGCCGGAA CGGTTGCCAA GTTGCCTGTA TACAACTTCG GGCAAGGTGA GCACCGCACG 
GTAATGAATG GGAGCGATGC GGACGAGTCC GGTGAGCGTG ATGAGCGAGA AATGGATGAT 
GATGATGACC TTTTCAGACA GTTGCAAGAC AGCAGTTATG TCCAGCATAG GGAATATATT 
CTTGATGAAC TTGTTTCTAA AGTCAGGAGG AATGGAGGTA AAGCTCCATT TAAAAATCCT 
CGTACACTTT TCAAGGGTCT TGCACTTGTT ATGCGAGACC AGGAAAAGAA TGTGCGACTC 
AAGTGCATTC AATTTATCAT GGACTTGGTT CCAATTCTCA AGCAGGAGCT TGATGCCTGC 
ATGATGCTCA TCATGCTTGA TCTGATATCA AGCTTTAGTG ATGCAAAGGT ATCAATACGG 
AAGGCTGCTG TTCAGACGCT GCATATGTAC ATGAAGCATA CAACTAACAT TGAGCAAATC 
TTCAACTCAA TTGTGAAGTA TGGGTTAGAA AATGAAGACC CCAAAATCAG AGCCGAAACA 
GCAGTTTCAA TTCCTGTTCT TCTGACAGGG GAATTTGCAA ACACTGACTT GTCAATTGTT 
ACTCGTGCAT TGGCTAGAAA GTTCACCGAT GATACTGATT TGATGGTAAC TGCAGGCGTA 
TCTTTGGACA GAATCTGTTC TTTGGTTGGG GAAGCATCAT TTAACTCGTA CATAGACAGC 
TTGCCAGCAA GAATCAAAAG TGGTTATTGC AAAAGAACAG GGAGACCTGA TCCTAGCCCA 
AGAATCCCCT CAGAAGGATA TTCAACCTCA GGATCTCTAG ATTGGAATTC CAACCGAACA 
ACGGAACCTA AAACTGTCCC AGGTGACGAG CGTAGATCGA GGCCTAAGCC AAATGTTGAA 
AAACAGTTTG GAATTGTACC ACAAGATGTG CTTGATCAAT TGTCAGACCA AGAACACTGG 
ACTGAGAGAG CATCTGGGGT ACAGGAGCTC AAAGAAATAG TTGTGGAACT CAAGGACATA 
TCACAGCTTA CACCTCACAT GATCAATTTC ATCAGTTTCC TGTGCAACTT ACTTGATGAC 
TCCAATTTCA AAGTTAGCTT GCACACCTTG GAAATATTTC AAGAACTGGT TCTCAAGTTC 
AACCAAAACA TTAAACCATT CGTTCGACAC ATAGTAAATG CTCTTTCAAA GCGCTTAGGA 
GACAACAAGA CAGTTATTAG ACAAGCAAAC ATGAAGGTGA TGATGCAGCT TATGCAAAGT 
GTAGGACCCA AACCTGTAGT TCATGTAGTA TCAGAGTTGA AGAACCAGCG GAAATCTGGA 
GTACGAGAGG AAGCTTTGAA CATTGTGATT GCAGCTCTTC TGACATTTCC TAGTTATGAC 
TTTGACCTGC CAGTCATTTG CCGAACACTG TCACAATGTC TAAATGATCA GAAGAGAAGG 
GTAAGGCAAG CAACGCTAGA ACTGTTTGCT GCCCTGGCTC AAGCAATGGG ACCAACTAGA 
ATAGGTCCTC TCATTGATGC TGTAGATCAG ATTGAAATGA AAACAGAAGA AGAAGGAGTC 
ATGAAGGCAG TTCAAGCAAG ACTGGCAAGG AGACAGCTAC CCAGATTAAA TGAGAATGGT 
TTAGTGGAGT ATGCATCTCA GACACCATCG TCAGCAGCAG CTGCAGGCAG AACAGCATCA 
TCAGCTGGTT ATGATACTGA ATGGATTTTG AAAGCTACAA GAAACTCAAG TGCAAAAGAA 
AGAGCCTTCA CTGATCCTGG GATAGAGCTT TCCCAACCAT CACCTACAAG AGCTAAACCA 
CCCATGAGTA ATGCTGCCAA CCCTGATTCA CAACCTCCTA AAAGGTTTCT GAGTGCAGGG 
AGAGGTCGCT CCAAACTACC ATGGGAAGAA GTGAGAGATG CAAGGGCTGG AGAGGATAAG 
GAAGATGGAG CTGGACAGAA GACCACAGGC AAAGGCAGAG TGCAAATAAA CAGTGCTCCA 
ACTCAGAAAC GTGAGGAGCC TGGGATAAGA CGACCGCCTA TCAAAGCTCG AAATACATGG 
AACGGAGGGG AAGATGTGTG GGACAATGCT AGACGTAGTC ACAACAACCT TACTGATATG 
GGATCTGACC AAGGTTCAGG TTCAGCTTCT AGTGGATCGT ACCGTCAGAT CCATCTTGAC 
AAACTCAAGA GAGCATCTCT AAATACCTAC CCTGGGAGGG GGCGAGGTAG CTGGGAGGAT 
GAGCCTCACG CATCAGGAAG CCACAGTGCA CCAGTCACAG AGAGAGACCT GAATCTTTGG 
AAAACTTTTT CTTTTGATGG AGATAATATA TTTGGTGGAC CAGATCCATT CATTGGCAAC 
ACAGCAGTCA TCCCCAAGAA GCGTGGCTCC CCCAAACAGC ACCCCTCCCA GCAGCATCAC 
CATCATCAGG AGGACTCTGA GCACCTCGCC CACCCCGCCC TCAGGCAACA GCTATCCTAC 
AGTGAGAAGT ACATGCCGTC TGTGGGAGCA TCCCGCTCCA GGAATGAGGA GGAAAGGGAG 
CAGCGAATGA ATCGGAAGAG CCTGTCAAAC TCCTGGCCTG ATCAGGGTAT CGGCATGGAT 
GATGGTCCAT CCTCGTCCTC CAATGCTGGG CGTAAGAAGA TGGACCTTAT GTTGGATTCT 
CTCCATGACT TCCATGCAAT TTCTCAATAT GACGTCCCTT CACCCAACAA ATCCAATTTC 
CCTGCCGGTA CCTCCCCCAT CTCACCCATC CCCCTGAAGG CTACACTGGC CCGATCAGCA 
GGGAGGAGGG GGCAAGGTAG AGCACCGTTG GTACCCCTAG AGAAGACTAC TAGTCCTCGG 
GATGATTATG ATGACTGGGA TTCTTATCAG GATACCCACC GATCGGATTG GACAGACACT 
GACGTTGTTC CTAATAGATC CTCACTGGAT AACATAAGAG GCAGCGCAGC CAAGAAGCGA 
GCAGAGAAAA TGGCACGGGG GCAGGATAAA TCCTCCGGTG GGAGCAACAG GGACTTAGAC 
GATAGTGGGT TATTCAGTCT ACAGTCTACG GGGCGCTTGG ATTTCGATGA TGGGACGCAT 
AATGGAGCTC AAACTACATA CGTCAAGAAG AAAAATCAAA CTGGAAAGAA AGGATCTGAC 
TCTCCATTCA GTTCTAAGCC ACGTATAGCA CGGTCAAACT CTGGGAAAGG TTCAAGACGA 
ACTGCTGCCG GGGAGCAACG CCAAGATGGC GGTAGCTCTT CAGCAAACCT GGAGTTTAAT 
CCCTCGGGAG GAGTGACGTT CAGAGACAAT GCGACGTCTG ACGTGAGCAT CATCGGTCAT 
GGCTTCGGGA ATGGACGGGG CATCGGTCAT AAGGACATTG ATCTCTCAGA TAATCATGTG 
CCTGCAGGCA AAGGACAGAA TTCTCGGGAT AGGAAGAGAA CAAAGGGGTC TATCCTGTCT 
CCTCTATCGA TGGCGTCTTT ACAGCATCCT GGAGGAGTTG ATCTGGATGA CAACGGGGCT 
TTGGTTGGGG AGGACTTTGC CATGGTTATT GGAAAAGGTG TGTTTGGTAA TGATGCTAAT 
ATAGTAGAGT ACCCACGATC AAATGGTGGA GAGAAGACAA TGAGGAGGAA AGAAAGCCAT 
GAAGACGGTG CCTTACCTGA GGTTATTGGT CTTCAGGTTC GGCATGATGA GGACAGTCCG 
GAAGAAAATG ACTTGTCTAT GTCCCTCTCT AAAGCAACAA TGGACAGGAT GGCAAGGAAA 
CAGATAGAGA AGAAGGAGGA GTTGGAGCAG CGCAAGGCGG ATCGTAAGGC CGAGAAGGAG 
CGTGCTGATG CAGAGAGACG CACTCAGGAG AAGGAGCGCG AGGTACAGAG AAGAGTTCTG 
GAGAAGGAAA TAGATGCCCA GAGAAAAGTG ATGCAGGAAC GTGAAAGGGA GCAGCAGCGG 
CTTGATTTCG AGAAGCAACA GAGGGAGAAG GAAAAGTTCA AGGAGGAGAG AGAGCGCCAG 
AGGGAGGAGA GGATGAGAGA GAGGGAGGAA CAGAAGAGAG AGCGAGAAGA AAGACTGCGG 
GAGGACAGAG AGCGTCAGAG AGCAGAGCGG GAAGAGAGGG AGCATCAGAA GGTAGAGAGA 
GAGAGGATAA GGGAGGAGCA ACAAGGCAAG GCTAGGGAGA GACAGAGGGA GAAACTCAAG 
CAGCTTGAGT TAGAGGGTGC CATGGATCAT GATGATGGGG GTGATCTTGC TATCAATGGA 
GCTATGCTAG ATAGCAGTAG ACCTAATCCT CCAGCCAGAG TAACGGCCAA GCCTCCTGCA 
GCTGCTACAC CACCTAAGAA GAAAAACAGT GGGATTTCTG TGACAAAGAA TTCTAGTGGT 
CCTCCTGCTG TAACATCACC ACCAGGGAAC GACGTCCAGA GCAGAGAGAT GAAACCAGTT 
AATAATGTAG ATGTAGCTGT CAAAGATTGT TTTAGATTGA TCGGACATGA AGACTGGGAA 
ATCAAGTTGG ATGGCTTGTT CATTATTCAA AGGCTAGCCA TTTTCCATAG CAATGACTTA 
GGAGGTCAGC TGCATGCAAT GGTCGTGGCT GTTCTTAATG AGGTCAAGAA TCTGAGGTCA 
ACGGTTGCTA GGGCTGCGTT ATCTACACTA GGAGATATGT TCACGTCATT TAAAACCAGT 
ATGGACAAGG ACTTGGATCC GATCTGTCGT ATCTTGTTAC CCAAGGCTGG TGAGAGCAAT 
GCTTTCATTC GAGAGGATGT TGACAAGGCC TTAGATGCCA TGGTCAAGAA CGCAAACCCA 
CAAAGAGTAT TAGGAGCATT GATCACTGCT GGAGCAAGTC ATAAGAGTGC TATGGTACGC 
AAGACGACCA GTGTGTTTCT GGATGCAGTG GTAGAGCGTA TGGGACCAGG AAGGATACTG 
AGTGGAGTGA AGGATGTCAC TGATAAGATC TTACCTGTTA CAGCTCAGCT AGCGCTCGAC 
AATGGACAAG AAACAAGGTA CTATGCGAGG AAGATGTTCT TTAATCTGAT GCACCATGAG 
GACTTTGATC GGTTGATTGA GAAACATGTC CCAGCTAAGA ATCTCAAGAG GATCAGAGAC 
ATCCTGGAAA ACCTCAAGAA TAAGGGTCTT GGTGATGTCC CATCAGATAC TTCATCTGCC 
AAAGCTCGTC GTAGCAATCC TGGTAGTCGA GCCAACTCTG GATCTCAATC ATATTCTAGG 
GATAGAAATG AATACAGTGA GGTGGACGGC ACCCCACCAA GCTCAGTCAA GAAAGGAAAG 
CGGTCTGGCG GAGGAGCCCG TGGAGAAGAG AACGAGACTA TCAAGAGTCT TTGTGAAGGT 
CTAGGAGCAT CGGATTGGAT GTCTAGGTTA CAAGCCATTG AGAGACTACA ATCCATGTGT 
GAAACAAATC AAGACTTGGT TGATGGGAGC TTAGTCAAGA TCTTTGATAA ATTCATCTCG 
AGGTTATCAG ACTCGAACAG TAAAGTGAAC ATAGCTGCTC TGACAACCAT GAAAGACATC 
GTACCTCGGC TTGGTGAATC CTTACCGGCT GTCGTCAATA ACCTCGTCCC AATCCTAGTC 
CAGAACCTTG CAGCAAAGAA CCCTAGCATC AGTCAGACCA GTAATGACAT CCTAGATCTC 
ATTCTGGAAC ATGTTGATAT GCTGGTATTA GTTCAGCCTT ACTCCAATGC TACTCAGTAT 
GGCAACGTGA GAGCCAGGCC AGCAATGGTA GAAAAGTTGT CCTACCTTGT GACCAAGGTG 
TACCCACGGA AGCAACAGAC GGTCGTCAGG AACGTTCTAC CTGTTCTATG GTACCTCCTG 
GGCAACATGA CAGGTAGCGG GGCGGTACCG GGGGGTTCAG GCAACCTACG TAGTGCCACA 
GCCGTCCTTG CTAGTAGCCT TCATGATCAG ATGGGTGAAG GCTTACTCCA ACATGCTCAG 
AACCTACCCC CTAGGAGTGT CAAGACACTC AAAGAACTCA TCGATGAAGA GACTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC580217 ( XM_030999676.1 ) cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin) -> XP_030855536.1 Strongylocentrotus purpuratus TOG array regulator of axonemal microtubules protein 1 (LOC580217), transcript variant X2, mRNA.

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CloneID	OSd65613
Clone ID Related Accession (Same CDS sequence)	XM_030999676.1
Accession Version	XM_030999676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Product	TOG array regulator of axonemal microtubules protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_022145611.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Strongylocentrotus purpuratus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAA CGGTTGCCAA GTTGCCTGTA TACAACTTCG GGCAAGGTGA GCACCGCACG 
GTAATGAATG GGAGCGATGC GGACGAGTCC GGTGAGCGTG ATGAGCGAGA AATGGATGAT 
GATGATGACC TTTTCAGACA GTTGCAAGAC AGCAGTTATG TCCAGCATAG GGAATATATT 
CTTGATGAAC TTGTTTCTAA AGTCAGGAGG AATGGAGGTA AAGCTCCATT TAAAAATCCT 
CGTACACTTT TCAAGGGTCT TGCACTTGTT ATGCGAGACC AGGAAAAGAA TGTGCGACTC 
AAGTGCATTC AATTTATCAT GGACTTGGTT CCAATTCTCA AGCAGGAGCT TGATGCCTGC 
ATGATGCTCA TCATGCTTGA TCTGATATCA AGCTTTAGTG ATGCAAAGGT ATCAATACGG 
AAGGCTGCTG TTCAGACGCT GCATATGTAC ATGAAGCATA CAACTAACAT TGAGCAAATC 
TTCAACTCAA TTGTGAAGTA TGGGTTAGAA AATGAAGACC CCAAAATCAG AGCCGAAACA 
GCAGTTTCAA TTCCTGTTCT TCTGACAGGG GAATTTGCAA ACACTGACTT GTCAATTGTT 
ACTCGTGCAT TGGCTAGAAA GTTCACCGAT GATACTGATT TGATGGTAAC TGCAGGCGTA 
TCTTTGGACA GAATCTGTTC TTTGGTTGGG GAAGCATCAT TTAACTCGTA CATAGACAGC 
TTGCCAGCAA GAATCAAAAG TGGTTATTGC AAAAGAACAG GGAGACCTGA TCCTAGCCCA 
AGAATCCCCT CAGAAGGATA TTCAACCTCA GGATCTCTAG ATTGGAATTC CAACCGAACA 
ACGGAACCTA AAACTGTCCC AGGTGACGAG CGTAGATCGA GGCCTAAGCC AAATGTTGAA 
AAACAGTTTG GAATTGTACC ACAAGATGTG CTTGATCAAT TGTCAGACCA AGAACACTGG 
ACTGAGAGAG CATCTGGGGT ACAGGAGCTC AAAGAAATAG TTGTGGAACT CAAGGACATA 
TCACAGCTTA CACCTCACAT GATCAATTTC ATCAGTTTCC TGTGCAACTT ACTTGATGAC 
TCCAATTTCA AAGTTAGCTT GCACACCTTG GAAATATTTC AAGAACTGGT TCTCAAGTTC 
AACCAAAACA TTAAACCATT CGTTCGACAC ATAGTAAATG CTCTTTCAAA GCGCTTAGGA 
GACAACAAGA CAGTTATTAG ACAAGCAAAC ATGAAGGTGA TGATGCAGCT TATGCAAAGT 
GTAGGACCCA AACCTGTAGT TCATGTAGTA TCAGAGTTGA AGAACCAGCG GAAATCTGGA 
GTACGAGAGG AAGCTTTGAA CATTGTGATT GCAGCTCTTC TGACATTTCC TAGTTATGAC 
TTTGACCTGC CAGTCATTTG CCGAACACTG TCACAATGTC TAAATGATCA GAAGAGAAGG 
GTAAGGCAAG CAACGCTAGA ACTGTTTGCT GCCCTGGCTC AAGCAATGGG ACCAACTAGA 
ATAGGTCCTC TCATTGATGC TGTAGATCAG ATTGAAATGA AAACAGAAGA AGAAGGAGTC 
ATGAAGGCAG TTCAAGCAAG ACTGGCAAGG AGACAGCTAC CCAGATTAAA TGAGAATGGT 
TTAGTGGAGT ATGCATCTCA GACACCATCG TCAGCAGCAG CTGCAGGCAG AACAGCATCA 
TCAGCTGGTT ATGATACTGA ATGGATTTTG AAAGCTACAA GAAACTCAAG TGCAAAAGAA 
AGAGCCTTCA CTGATCCTGG GATAGAGCTT TCCCAACCAT CACCTACAAG AGCTAAACCA 
CCCATGAGTA ATGCTGCCAA CCCTGATTCA CAACCTCCTA AAAGGTTTCT GAGTGCAGGG 
AGAGGTCGCT CCAAACTACC ATGGGAAGAA GTGAGAGATG CAAGGGCTGG AGAGGATAAG 
GAAGATGGAG CTGGACAGAA GACCACAGGC AAAGGCAGAG TGCAAATAAA CAGTGCTCCA 
ACTCAGAAAC GTGAGGAGCC TGGGATAAGA CGACCGCCTA TCAAAGCTCG AAATACATGG 
AACGGAGGGG AAGATGTGTG GGACAATGCT AGACGTAGTC ACAACAACCT TACTGATATG 
GGATCTGACC AAGGTTCAGG TTCAGCTTCT AGTGGATCGT ACCGTCAGAT CCATCTTGAC 
AAACTCAAGA GAGCATCTCT AAATACCTAC CCTGGGAGGG GGCGAGGTAG CTGGGAGGAT 
GAGCCTCACG CATCAGGAAG CCACAGTGCA CCAGTCACAG AGAGAGGTGG ACCAGATCCA 
TTCATTGGCA ACACAGCAGT CATCCCCAAG AAGCGTGGCT CCCCCAAACA GCACCCCTCC 
CAGCAGCATC ACCATCATCA GGAGGACTCT GAGCACCTCG CCCACCCCGC CCTCAGGCAA 
CAGCTATCCT ACAGTGAGAA GTACATGCCG TCTGTGGGAG CATCCCGCTC CAGGAATGAG 
GAGGAAAGGG AGCAGCGAAT GAATCGGAAG AGCCTGTCAA ACTCCTGGCC TGATCAGGGT 
ATCGGCATGG ATGATGGTCC ATCCTCGTCC TCCAATGCTG GGCGTAAGAA GATGGACCTT 
ATGTTGGATT CTCTCCATGA CTTCCATGCA ATTTCTCAAT ATGACGTCCC TTCACCCAAC 
AAATCCAATT TCCCTGCCGG TACCTCCCCC ATCTCACCCA TCCCCCTGAA GGCTACACTG 
GCCCGATCAG CAGGGAGGAG GGGGCAAGGT AGAGCACCGT TGGTACCCCT AGAGAAGACT 
ACTAGTCCTC GGGATGATTA TGATGACTGG GATTCTTATC AGGATACCCA CCGATCGGAT 
TGGACAGACA CTGACGTTGT TCCTAATAGA TCCTCACTGG ATAACATAAG AGGCAGCGCA 
GCCAAGAAGC GAGCAGAGAA AATGGCACGG GGGCAGGATA AATCCTCCGG TGGGAGCAAC 
AGGGACTTAG ACGATAGTGG GTTATTCAGT CTACAGTCTA CGGGGCGCTT GGATTTCGAT 
GATGGGACGC ATAATGGAGC TCAAACTACA TACGTCAAGA AGAAAAATCA AACTGGAAAG 
AAAGGATCTG ACTCTCCATT CAGTTCTAAG CCACGTATAG CACGGTCAAA CTCTGGGAAA 
GGTTCAAGAC GAACTGCTGC CGGGGAGCAA CGCCAAGATG GCGGTAGCTC TTCAGCAAAC 
CTGGAGTTTA ATCCCTCGGG AGGAGTGACG TTCAGAGACA ATGCGACGTC TGACGTGAGC 
ATCATCGGTC ATGGCTTCGG GAATGGACGG GGCATCGGTC ATAAGGACAT TGATCTCTCA 
GATAATCATG TGCCTGCAGG CAAAGGACAG AATTCTCGGG ATAGGAAGAG AACAAAGGGG 
TCTATCCTGT CTCCTCTATC GATGGCGTCT TTACAGCATC CTGGAGGAGT TGATCTGGAT 
GACAACGGGG CTTTGGTTGG GGAGGACTTT GCCATGGTTA TTGGAAAAGG TGTGTTTGGT 
AATGATGCTA ATATAGTAGA GTACCCACGA TCAAATGGTG GAGAGAAGAC AATGAGGAGG 
AAAGAAAGCC ATGAAGACGG TGCCTTACCT GAGGTTATTG GTCTTCAGGT TCGGCATGAT 
GAGGACAGTC CGGAAGAAAA TGACTTGTCT ATGTCCCTCT CTAAAGCAAC AATGGACAGG 
ATGGCAAGGA AACAGATAGA GAAGAAGGAG GAGTTGGAGC AGCGCAAGGC GGATCGTAAG 
GCCGAGAAGG AGCGTGCTGA TGCAGAGAGA CGCACTCAGG AGAAGGAGCG CGAGGTACAG 
AGAAGAGTTC TGGAGAAGGA AATAGATGCC CAGAGAAAAG TGATGCAGGA ACGTGAAAGG 
GAGCAGCAGC GGCTTGATTT CGAGAAGCAA CAGAGGGAGA AGGAAAAGTT CAAGGAGGAG 
AGAGAGCGCC AGAGGGAGGA GAGGATGAGA GAGAGGGAGG AACAGAAGAG AGAGCGAGAA 
GAAAGACTGC GGGAGGACAG AGAGCGTCAG AGAGCAGAGC GGGAAGAGAG GGAGCATCAG 
AAGGTAGAGA GAGAGAGGAT AAGGGAGGAG CAACAAGGCA AGGCTAGGGA GAGACAGAGG 
GAGAAACTCA AGCAGCTTGA GTTAGAGGGT GCCATGGATC ATGATGATGG GGGTGATCTT 
GCTATCAATG GAGCTATGCT AGATAGCAGT AGACCTAATC CTCCAGCCAG AGTAACGGCC 
AAGCCTCCTG CAGCTGCTAC ACCACCTAAG AAGAAAAACA GTGGGATTTC TGTGACAAAG 
AATTCTAGTG GTCCTCCTGC TGTAACATCA CCACCAGGGA ACGACGTCCA GAGCAGAGAG 
ATGAAACCAG TTAATAATGT AGATGTAGCT GTCAAAGATT GTTTTAGATT GATCGGACAT 
GAAGACTGGG AAATCAAGTT GGATGGCTTG TTCATTATTC AAAGGCTAGC CATTTTCCAT 
AGCAATGACT TAGGAGGTCA GCTGCATGCA ATGGTCGTGG CTGTTCTTAA TGAGGTCAAG 
AATCTGAGGT CAACGGTTGC TAGGGCTGCG TTATCTACAC TAGGAGATAT GTTCACGTCA 
TTTAAAACCA GTATGGACAA GGACTTGGAT CCGATCTGTC GTATCTTGTT ACCCAAGGCT 
GGTGAGAGCA ATGCTTTCAT TCGAGAGGAT GTTGACAAGG CCTTAGATGC CATGGTCAAG 
AACGCAAACC CACAAAGAGT ATTAGGAGCA TTGATCACTG CTGGAGCAAG TCATAAGAGT 
GCTATGGTAC GCAAGACGAC CAGTGTGTTT CTGGATGCAG TGGTAGAGCG TATGGGACCA 
GGAAGGATAC TGAGTGGAGT GAAGGATGTC ACTGATAAGA TCTTACCTGT TACAGCTCAG 
CTAGCGCTCG ACAATGGACA AGAAACAAGG TACTATGCGA GGAAGATGTT CTTTAATCTG 
ATGCACCATG AGGACTTTGA TCGGTTGATT GAGAAACATG TCCCAGCTAA GAATCTCAAG 
AGGATCAGAG ACATCCTGGA AAACCTCAAG AATAAGGGTC TTGGTGATGT CCCATCAGAT 
ACTTCATCTG CCAAAGCTCG TCGTAGCAAT CCTGGTAGTC GAGCCAACTC TGGATCTCAA 
TCATATTCTA GGGATAGAAA TGAATACAGT GAGGTGGACG GCACCCCACC AAGCTCAGTC 
AAGAAAGGAA AGCGGTCTGG CGGAGGAGCC CGTGGAGAAG AGAACGAGAC TATCAAGAGT 
CTTTGTGAAG GTCTAGGAGC ATCGGATTGG ATGTCTAGGT TACAAGCCAT TGAGAGACTA 
CAATCCATGT GTGAAACAAA TCAAGACTTG GTTGATGGGA GCTTAGTCAA GATCTTTGAT 
AAATTCATCT CGAGGTTATC AGACTCGAAC AGTAAAGTGA ACATAGCTGC TCTGACAACC 
ATGAAAGACA TCGTACCTCG GCTTGGTGAA TCCTTACCGG CTGTCGTCAA TAACCTCGTC 
CCAATCCTAG TCCAGAACCT TGCAGCAAAG AACCCTAGCA TCAGTCAGAC CAGTAATGAC 
ATCCTAGATC TCATTCTGGA ACATGTTGAT ATGCTGGTAT TAGTTCAGCC TTACTCCAAT 
GCTACTCAGT ATGGCAACGT GAGAGCCAGG CCAGCAATGG TAGAAAAGTT GTCCTACCTT 
GTGACCAAGG TGTACCCACG GAAGCAACAG ACGGTCGTCA GGAACGTTCT ACCTGTTCTA 
TGGTACCTCC TGGGCAACAT GACAGGTAGC GGGGCGGTAC CGGGGGGTTC AGGCAACCTA 
CGTAGTGCCA CAGCCGTCCT TGCTAGTAGC CTTCATGATC AGATGGGTGA AGGCTTACTC 
CAACATGCTC AGAACCTACC CCCTAGGAGT GTCAAGACAC TCAAAGAACT CATCGATGAA 
GAGACTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030855536.1
CDS	225..6116
Translation	MAGTVAKLPVYNFGQGEHRTVMNGSDADESGERDEREMDDDDDLFRQLQDSSYVQHREYILDELVSKVRRNGGKAPFKNPRTLFKGLALVMRDQEKNVRLKCIQFIMDLVPILKQELDACMMLIMLDLISSFSDAKVSIRKAAVQTLHMYMKHTTNIEQIFNSIVKYGLENEDPKIRAETAVSIPVLLTGEFANTDLSIVTRALARKFTDDTDLMVTAGVSLDRICSLVGEASFNSYIDSLPARIKSGYCKRTGRPDPSPRIPSEGYSTSGSLDWNSNRTTEPKTVPGDERRSRPKPNVEKQFGIVPQDVLDQLSDQEHWTERASGVQELKEIVVELKDISQLTPHMINFISFLCNLLDDSNFKVSLHTLEIFQELVLKFNQNIKPFVRHIVNALSKRLGDNKTVIRQANMKVMMQLMQSVGPKPVVHVVSELKNQRKSGVREEALNIVIAALLTFPSYDFDLPVICRTLSQCLNDQKRRVRQATLELFAALAQAMGPTRIGPLIDAVDQIEMKTEEEGVMKAVQARLARRQLPRLNENGLVEYASQTPSSAAAAGRTASSAGYDTEWILKATRNSSAKERAFTDPGIELSQPSPTRAKPPMSNAANPDSQPPKRFLSAGRGRSKLPWEEVRDARAGEDKEDGAGQKTTGKGRVQINSAPTQKREEPGIRRPPIKARNTWNGGEDVWDNARRSHNNLTDMGSDQGSGSASSGSYRQIHLDKLKRASLNTYPGRGRGSWEDEPHASGSHSAPVTERGGPDPFIGNTAVIPKKRGSPKQHPSQQHHHHQEDSEHLAHPALRQQLSYSEKYMPSVGASRSRNEEEREQRMNRKSLSNSWPDQGIGMDDGPSSSSNAGRKKMDLMLDSLHDFHAISQYDVPSPNKSNFPAGTSPISPIPLKATLARSAGRRGQGRAPLVPLEKTTSPRDDYDDWDSYQDTHRSDWTDTDVVPNRSSLDNIRGSAAKKRAEKMARGQDKSSGGSNRDLDDSGLFSLQSTGRLDFDDGTHNGAQTTYVKKKNQTGKKGSDSPFSSKPRIARSNSGKGSRRTAAGEQRQDGGSSSANLEFNPSGGVTFRDNATSDVSIIGHGFGNGRGIGHKDIDLSDNHVPAGKGQNSRDRKRTKGSILSPLSMASLQHPGGVDLDDNGALVGEDFAMVIGKGVFGNDANIVEYPRSNGGEKTMRRKESHEDGALPEVIGLQVRHDEDSPEENDLSMSLSKATMDRMARKQIEKKEELEQRKADRKAEKERADAERRTQEKEREVQRRVLEKEIDAQRKVMQEREREQQRLDFEKQQREKEKFKEERERQREERMREREEQKREREERLREDRERQRAEREEREHQKVERERIREEQQGKARERQREKLKQLELEGAMDHDDGGDLAINGAMLDSSRPNPPARVTAKPPAAATPPKKKNSGISVTKNSSGPPAVTSPPGNDVQSREMKPVNNVDVAVKDCFRLIGHEDWEIKLDGLFIIQRLAIFHSNDLGGQLHAMVVAVLNEVKNLRSTVARAALSTLGDMFTSFKTSMDKDLDPICRILLPKAGESNAFIREDVDKALDAMVKNANPQRVLGALITAGASHKSAMVRKTTSVFLDAVVERMGPGRILSGVKDVTDKILPVTAQLALDNGQETRYYARKMFFNLMHHEDFDRLIEKHVPAKNLKRIRDILENLKNKGLGDVPSDTSSAKARRSNPGSRANSGSQSYSRDRNEYSEVDGTPPSSVKKGKRSGGGARGEENETIKSLCEGLGASDWMSRLQAIERLQSMCETNQDLVDGSLVKIFDKFISRLSDSNSKVNIAALTTMKDIVPRLGESLPAVVNNLVPILVQNLAAKNPSISQTSNDILDLILEHVDMLVLVQPYSNATQYGNVRARPAMVEKLSYLVTKVYPRKQQTVVRNVLPVLWYLLGNMTGSGAVPGGSGNLRSATAVLASSLHDQMGEGLLQHAQNLPPRSVKTLKELIDEETT

Target ORF information:

RefSeq Version	XM_030999676.1
Organism	Strongylocentrotus purpuratus(purple sea urchin)
Definition	Strongylocentrotus purpuratus TOG array regulator of axonemal microtubules protein 1 (LOC580217), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030999676.1

ORF Insert Sequence:

ATGGCCGGAA CGGTTGCCAA GTTGCCTGTA TACAACTTCG GGCAAGGTGA GCACCGCACG 
GTAATGAATG GGAGCGATGC GGACGAGTCC GGTGAGCGTG ATGAGCGAGA AATGGATGAT 
GATGATGACC TTTTCAGACA GTTGCAAGAC AGCAGTTATG TCCAGCATAG GGAATATATT 
CTTGATGAAC TTGTTTCTAA AGTCAGGAGG AATGGAGGTA AAGCTCCATT TAAAAATCCT 
CGTACACTTT TCAAGGGTCT TGCACTTGTT ATGCGAGACC AGGAAAAGAA TGTGCGACTC 
AAGTGCATTC AATTTATCAT GGACTTGGTT CCAATTCTCA AGCAGGAGCT TGATGCCTGC 
ATGATGCTCA TCATGCTTGA TCTGATATCA AGCTTTAGTG ATGCAAAGGT ATCAATACGG 
AAGGCTGCTG TTCAGACGCT GCATATGTAC ATGAAGCATA CAACTAACAT TGAGCAAATC 
TTCAACTCAA TTGTGAAGTA TGGGTTAGAA AATGAAGACC CCAAAATCAG AGCCGAAACA 
GCAGTTTCAA TTCCTGTTCT TCTGACAGGG GAATTTGCAA ACACTGACTT GTCAATTGTT 
ACTCGTGCAT TGGCTAGAAA GTTCACCGAT GATACTGATT TGATGGTAAC TGCAGGCGTA 
TCTTTGGACA GAATCTGTTC TTTGGTTGGG GAAGCATCAT TTAACTCGTA CATAGACAGC 
TTGCCAGCAA GAATCAAAAG TGGTTATTGC AAAAGAACAG GGAGACCTGA TCCTAGCCCA 
AGAATCCCCT CAGAAGGATA TTCAACCTCA GGATCTCTAG ATTGGAATTC CAACCGAACA 
ACGGAACCTA AAACTGTCCC AGGTGACGAG CGTAGATCGA GGCCTAAGCC AAATGTTGAA 
AAACAGTTTG GAATTGTACC ACAAGATGTG CTTGATCAAT TGTCAGACCA AGAACACTGG 
ACTGAGAGAG CATCTGGGGT ACAGGAGCTC AAAGAAATAG TTGTGGAACT CAAGGACATA 
TCACAGCTTA CACCTCACAT GATCAATTTC ATCAGTTTCC TGTGCAACTT ACTTGATGAC 
TCCAATTTCA AAGTTAGCTT GCACACCTTG GAAATATTTC AAGAACTGGT TCTCAAGTTC 
AACCAAAACA TTAAACCATT CGTTCGACAC ATAGTAAATG CTCTTTCAAA GCGCTTAGGA 
GACAACAAGA CAGTTATTAG ACAAGCAAAC ATGAAGGTGA TGATGCAGCT TATGCAAAGT 
GTAGGACCCA AACCTGTAGT TCATGTAGTA TCAGAGTTGA AGAACCAGCG GAAATCTGGA 
GTACGAGAGG AAGCTTTGAA CATTGTGATT GCAGCTCTTC TGACATTTCC TAGTTATGAC 
TTTGACCTGC CAGTCATTTG CCGAACACTG TCACAATGTC TAAATGATCA GAAGAGAAGG 
GTAAGGCAAG CAACGCTAGA ACTGTTTGCT GCCCTGGCTC AAGCAATGGG ACCAACTAGA 
ATAGGTCCTC TCATTGATGC TGTAGATCAG ATTGAAATGA AAACAGAAGA AGAAGGAGTC 
ATGAAGGCAG TTCAAGCAAG ACTGGCAAGG AGACAGCTAC CCAGATTAAA TGAGAATGGT 
TTAGTGGAGT ATGCATCTCA GACACCATCG TCAGCAGCAG CTGCAGGCAG AACAGCATCA 
TCAGCTGGTT ATGATACTGA ATGGATTTTG AAAGCTACAA GAAACTCAAG TGCAAAAGAA 
AGAGCCTTCA CTGATCCTGG GATAGAGCTT TCCCAACCAT CACCTACAAG AGCTAAACCA 
CCCATGAGTA ATGCTGCCAA CCCTGATTCA CAACCTCCTA AAAGGTTTCT GAGTGCAGGG 
AGAGGTCGCT CCAAACTACC ATGGGAAGAA GTGAGAGATG CAAGGGCTGG AGAGGATAAG 
GAAGATGGAG CTGGACAGAA GACCACAGGC AAAGGCAGAG TGCAAATAAA CAGTGCTCCA 
ACTCAGAAAC GTGAGGAGCC TGGGATAAGA CGACCGCCTA TCAAAGCTCG AAATACATGG 
AACGGAGGGG AAGATGTGTG GGACAATGCT AGACGTAGTC ACAACAACCT TACTGATATG 
GGATCTGACC AAGGTTCAGG TTCAGCTTCT AGTGGATCGT ACCGTCAGAT CCATCTTGAC 
AAACTCAAGA GAGCATCTCT AAATACCTAC CCTGGGAGGG GGCGAGGTAG CTGGGAGGAT 
GAGCCTCACG CATCAGGAAG CCACAGTGCA CCAGTCACAG AGAGAGGTGG ACCAGATCCA 
TTCATTGGCA ACACAGCAGT CATCCCCAAG AAGCGTGGCT CCCCCAAACA GCACCCCTCC 
CAGCAGCATC ACCATCATCA GGAGGACTCT GAGCACCTCG CCCACCCCGC CCTCAGGCAA 
CAGCTATCCT ACAGTGAGAA GTACATGCCG TCTGTGGGAG CATCCCGCTC CAGGAATGAG 
GAGGAAAGGG AGCAGCGAAT GAATCGGAAG AGCCTGTCAA ACTCCTGGCC TGATCAGGGT 
ATCGGCATGG ATGATGGTCC ATCCTCGTCC TCCAATGCTG GGCGTAAGAA GATGGACCTT 
ATGTTGGATT CTCTCCATGA CTTCCATGCA ATTTCTCAAT ATGACGTCCC TTCACCCAAC 
AAATCCAATT TCCCTGCCGG TACCTCCCCC ATCTCACCCA TCCCCCTGAA GGCTACACTG 
GCCCGATCAG CAGGGAGGAG GGGGCAAGGT AGAGCACCGT TGGTACCCCT AGAGAAGACT 
ACTAGTCCTC GGGATGATTA TGATGACTGG GATTCTTATC AGGATACCCA CCGATCGGAT 
TGGACAGACA CTGACGTTGT TCCTAATAGA TCCTCACTGG ATAACATAAG AGGCAGCGCA 
GCCAAGAAGC GAGCAGAGAA AATGGCACGG GGGCAGGATA AATCCTCCGG TGGGAGCAAC 
AGGGACTTAG ACGATAGTGG GTTATTCAGT CTACAGTCTA CGGGGCGCTT GGATTTCGAT 
GATGGGACGC ATAATGGAGC TCAAACTACA TACGTCAAGA AGAAAAATCA AACTGGAAAG 
AAAGGATCTG ACTCTCCATT CAGTTCTAAG CCACGTATAG CACGGTCAAA CTCTGGGAAA 
GGTTCAAGAC GAACTGCTGC CGGGGAGCAA CGCCAAGATG GCGGTAGCTC TTCAGCAAAC 
CTGGAGTTTA ATCCCTCGGG AGGAGTGACG TTCAGAGACA ATGCGACGTC TGACGTGAGC 
ATCATCGGTC ATGGCTTCGG GAATGGACGG GGCATCGGTC ATAAGGACAT TGATCTCTCA 
GATAATCATG TGCCTGCAGG CAAAGGACAG AATTCTCGGG ATAGGAAGAG AACAAAGGGG 
TCTATCCTGT CTCCTCTATC GATGGCGTCT TTACAGCATC CTGGAGGAGT TGATCTGGAT 
GACAACGGGG CTTTGGTTGG GGAGGACTTT GCCATGGTTA TTGGAAAAGG TGTGTTTGGT 
AATGATGCTA ATATAGTAGA GTACCCACGA TCAAATGGTG GAGAGAAGAC AATGAGGAGG 
AAAGAAAGCC ATGAAGACGG TGCCTTACCT GAGGTTATTG GTCTTCAGGT TCGGCATGAT 
GAGGACAGTC CGGAAGAAAA TGACTTGTCT ATGTCCCTCT CTAAAGCAAC AATGGACAGG 
ATGGCAAGGA AACAGATAGA GAAGAAGGAG GAGTTGGAGC AGCGCAAGGC GGATCGTAAG 
GCCGAGAAGG AGCGTGCTGA TGCAGAGAGA CGCACTCAGG AGAAGGAGCG CGAGGTACAG 
AGAAGAGTTC TGGAGAAGGA AATAGATGCC CAGAGAAAAG TGATGCAGGA ACGTGAAAGG 
GAGCAGCAGC GGCTTGATTT CGAGAAGCAA CAGAGGGAGA AGGAAAAGTT CAAGGAGGAG 
AGAGAGCGCC AGAGGGAGGA GAGGATGAGA GAGAGGGAGG AACAGAAGAG AGAGCGAGAA 
GAAAGACTGC GGGAGGACAG AGAGCGTCAG AGAGCAGAGC GGGAAGAGAG GGAGCATCAG 
AAGGTAGAGA GAGAGAGGAT AAGGGAGGAG CAACAAGGCA AGGCTAGGGA GAGACAGAGG 
GAGAAACTCA AGCAGCTTGA GTTAGAGGGT GCCATGGATC ATGATGATGG GGGTGATCTT 
GCTATCAATG GAGCTATGCT AGATAGCAGT AGACCTAATC CTCCAGCCAG AGTAACGGCC 
AAGCCTCCTG CAGCTGCTAC ACCACCTAAG AAGAAAAACA GTGGGATTTC TGTGACAAAG 
AATTCTAGTG GTCCTCCTGC TGTAACATCA CCACCAGGGA ACGACGTCCA GAGCAGAGAG 
ATGAAACCAG TTAATAATGT AGATGTAGCT GTCAAAGATT GTTTTAGATT GATCGGACAT 
GAAGACTGGG AAATCAAGTT GGATGGCTTG TTCATTATTC AAAGGCTAGC CATTTTCCAT 
AGCAATGACT TAGGAGGTCA GCTGCATGCA ATGGTCGTGG CTGTTCTTAA TGAGGTCAAG 
AATCTGAGGT CAACGGTTGC TAGGGCTGCG TTATCTACAC TAGGAGATAT GTTCACGTCA 
TTTAAAACCA GTATGGACAA GGACTTGGAT CCGATCTGTC GTATCTTGTT ACCCAAGGCT 
GGTGAGAGCA ATGCTTTCAT TCGAGAGGAT GTTGACAAGG CCTTAGATGC CATGGTCAAG 
AACGCAAACC CACAAAGAGT ATTAGGAGCA TTGATCACTG CTGGAGCAAG TCATAAGAGT 
GCTATGGTAC GCAAGACGAC CAGTGTGTTT CTGGATGCAG TGGTAGAGCG TATGGGACCA 
GGAAGGATAC TGAGTGGAGT GAAGGATGTC ACTGATAAGA TCTTACCTGT TACAGCTCAG 
CTAGCGCTCG ACAATGGACA AGAAACAAGG TACTATGCGA GGAAGATGTT CTTTAATCTG 
ATGCACCATG AGGACTTTGA TCGGTTGATT GAGAAACATG TCCCAGCTAA GAATCTCAAG 
AGGATCAGAG ACATCCTGGA AAACCTCAAG AATAAGGGTC TTGGTGATGT CCCATCAGAT 
ACTTCATCTG CCAAAGCTCG TCGTAGCAAT CCTGGTAGTC GAGCCAACTC TGGATCTCAA 
TCATATTCTA GGGATAGAAA TGAATACAGT GAGGTGGACG GCACCCCACC AAGCTCAGTC 
AAGAAAGGAA AGCGGTCTGG CGGAGGAGCC CGTGGAGAAG AGAACGAGAC TATCAAGAGT 
CTTTGTGAAG GTCTAGGAGC ATCGGATTGG ATGTCTAGGT TACAAGCCAT TGAGAGACTA 
CAATCCATGT GTGAAACAAA TCAAGACTTG GTTGATGGGA GCTTAGTCAA GATCTTTGAT 
AAATTCATCT CGAGGTTATC AGACTCGAAC AGTAAAGTGA ACATAGCTGC TCTGACAACC 
ATGAAAGACA TCGTACCTCG GCTTGGTGAA TCCTTACCGG CTGTCGTCAA TAACCTCGTC 
CCAATCCTAG TCCAGAACCT TGCAGCAAAG AACCCTAGCA TCAGTCAGAC CAGTAATGAC 
ATCCTAGATC TCATTCTGGA ACATGTTGAT ATGCTGGTAT TAGTTCAGCC TTACTCCAAT 
GCTACTCAGT ATGGCAACGT GAGAGCCAGG CCAGCAATGG TAGAAAAGTT GTCCTACCTT 
GTGACCAAGG TGTACCCACG GAAGCAACAG ACGGTCGTCA GGAACGTTCT ACCTGTTCTA 
TGGTACCTCC TGGGCAACAT GACAGGTAGC GGGGCGGTAC CGGGGGGTTC AGGCAACCTA 
CGTAGTGCCA CAGCCGTCCT TGCTAGTAGC CTTCATGATC AGATGGGTGA AGGCTTACTC 
CAACATGCTC AGAACCTACC CCCTAGGAGT GTCAAGACAC TCAAAGAACT CATCGATGAA 
GAGACTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC580217 cDNA ORF clone, Strongylocentrotus purpuratus(purple sea urchin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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