LOC100142296 cDNA ORF clone, Tribolium castaneum(red flour beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100142296
Entrez Gene ID	100142296
Full Name	RNA polymerase II-associated factor 1 homolog
Gene Type	protein-coding
Organism	Tribolium castaneum(red flour beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015985189.1	XP_015840675.1	LOW QUALITY PROTEIN: RNA polymerase II-associated factor 1 homolog

Pathways from BioSystems
Gene Ontology

The following LOC100142296 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100142296 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTg01372	XM_015985189.1 Latest version!	Tribolium castaneum RNA polymerase II-associated factor 1 homolog (LOC100142296), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100142296 ( XM_015985189.1 ) cDNA ORF clone, Tribolium castaneum(red flour beetle) -> XP_015840675.1 Tribolium castaneum RNA polymerase II-associated factor 1 homolog (LOC100142296), mRNA.

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CloneID	OTg01372
Clone ID Related Accession (Same CDS sequence)	XM_015985189.1
Accession Version	XM_015985189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Tribolium castaneum(red flour beetle)
Product	LOW QUALITY PROTEIN: RNA polymerase II-associated factor 1 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015450135.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tribolium castaneum Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCCTCCAA CGATACAAAA TGGAACTTCC ACGCAAGAAA AACGGCCTGT TAAACCAGCA 
GAAAAAAGGT CCGACTTGAT ATGCAGGGTG AAATATTGCA ACACGCTGNC TGATATCCCG 
TTTGATTTGA AATTTATCTC ATACCCGTTT GATTCATCAA GGTTTATACA ATTTAATCCA 
ACCTCGTTGG AGAGAAATTA TCGGTATGAA GTGCTCACTG AACATGATTT GGGCGTTGCC 
ATTGACTTGA TTAACAAAGA TATTTACGCT GTGGAACCAG GTGCTATGCT TGATCCAGCT 
GATGAGAAAC TCCTAGAAGA AGATATCCTA ACACCTCAAG ACTCGAAAAG GTCCAGACAC 
CATGCAAGAT CTGTCTCTTG GCTTCGCAGG ACTGAATACA TTTCAACAGA ACAGACTCGA 
TTCCAGCCAC AAAGTATGGA TAAAGTCGAA GCGAAAGTTG GCTACAGTAT TAAGAAGAAT 
TTGAAGAATG AAACATTGTA CATGGACCGT GATTCACAAA TCAAGGCTAT TGAGAAAACG 
TTCAGTGATA CAACGAATCC GATTGAAAAG CATTACAGCA AGCCGAATGT GACTGCTGTT 
GAAGTATTGC CAGTGTTTCC CGATTTCAAT CTGTGGAAAT ATCCATGTGC TCAGGTTATT 
TTTGATTCAG ATCCAGCGCC TGTTGGTAAA CAAGTGCCCG CGCAGATTGA GGAAATGTCA 
CAAGCTATGA TCAGAGGTGT GATGGACGAA CGTGGCGAAC AATTCGTCGC CTACTTCCTC 
CCAACTGAAG AGACTCTAGT TAAAAGACGG GAAGACTTTG CTAACGAGAT ACCATACCAA 
GACGATGAAG AATATGAGTA TAAAATGGCA AGAGAATACA ACTGGAACGT GAAGAGTAAA 
GCAAGCAAAG GTTACGAAGA AAATTACTTC TTCGTAATCA GACAAGATGG TGCCTACTAT 
AATGAGCTAG AAACCCGTGT CAGATTGAGT AAACGTCGCC AGAAAGTTGG CCAACCTGCC 
AGCAACACCC GTCTTATCGT CAAACACCGA CCAATGGATG CGAACGAATT CCGAATGCAA 
AGATACCGCG AAAAACAACT CGAACCTGCA GGCGATGAAG ACGAAGAAAT GGAAATGGAA 
CAAAACGAAG AACCACAAGA AAACGAAAAA GAAAGCGACG CGGAATCGTC AAGATCCAAA 
TCACCGTCAC GTTCAAGATC TCCGTCTAGA TCCCGATCAC AGTCTAAATC TCCCTCACGG 
TCCAGATCCA GATCCAAATC GCGATCAAGA TCCAGATCCA AATCGCGGTC AAGATCCAGA 
TCAAAATCTG CGTCTAGATC CAGATCACGT TCACGCTCGG CTTCCGGATC GCGATCTCCT 
TCCAAATCGC CGTCACGATC CAGATCCGGA TCCAAATCAC GATCACCATC ACGTTCCAAA 
TCACGGAGTA AATCACCGTC CAGATCCAGA TCCGGATCGC GGTCAAGATC CGGATCCCGA 
TCTGGATCCG AATCCGGCAG CAAGTCTGGA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015840675.1
CDS	119..1654
Translation	MPPTIQNGTSTQEKRPVKPAEKRSDLICRVKYCNTLnullDIPFDLKFISYPFDSSRFIQFNPTSLERNYRYEVLTEHDLGVAIDLINKDIYAVEPGAMLDPADEKLLEEDILTPQDSKRSRHHARSVSWLRRTEYISTEQTRFQPQSMDKVEAKVGYSIKKNLKNETLYMDRDSQIKAIEKTFSDTTNPIEKHYSKPNVTAVEVLPVFPDFNLWKYPCAQVIFDSDPAPVGKQVPAQIEEMSQAMIRGVMDERGEQFVAYFLPTEETLVKRREDFANEIPYQDDEEYEYKMAREYNWNVKSKASKGYEENYFFVIRQDGAYYNELETRVRLSKRRQKVGQPASNTRLIVKHRPMDANEFRMQRYREKQLEPAGDEDEEMEMEQNEEPQENEKESDAESSRSKSPSRSRSPSRSRSQSKSPSRSRSRSKSRSRSRSKSRSRSRSKSASRSRSRSRSASGSRSPSKSPSRSRSGSKSRSPSRSKSRSKSPSRSRSGSRSRSGSRSGSESGSKSGQ

Target ORF information:

RefSeq Version	XM_015985189.1
Organism	Tribolium castaneum(red flour beetle)
Definition	Tribolium castaneum RNA polymerase II-associated factor 1 homolog (LOC100142296), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015985189.1

ORF Insert Sequence:

ATGCCTCCAA CGATACAAAA TGGAACTTCC ACGCAAGAAA AACGGCCTGT TAAACCAGCA 
GAAAAAAGGT CCGACTTGAT ATGCAGGGTG AAATATTGCA ACACGCTGNC TGATATCCCG 
TTTGATTTGA AATTTATCTC ATACCCGTTT GATTCATCAA GGTTTATACA ATTTAATCCA 
ACCTCGTTGG AGAGAAATTA TCGGTATGAA GTGCTCACTG AACATGATTT GGGCGTTGCC 
ATTGACTTGA TTAACAAAGA TATTTACGCT GTGGAACCAG GTGCTATGCT TGATCCAGCT 
GATGAGAAAC TCCTAGAAGA AGATATCCTA ACACCTCAAG ACTCGAAAAG GTCCAGACAC 
CATGCAAGAT CTGTCTCTTG GCTTCGCAGG ACTGAATACA TTTCAACAGA ACAGACTCGA 
TTCCAGCCAC AAAGTATGGA TAAAGTCGAA GCGAAAGTTG GCTACAGTAT TAAGAAGAAT 
TTGAAGAATG AAACATTGTA CATGGACCGT GATTCACAAA TCAAGGCTAT TGAGAAAACG 
TTCAGTGATA CAACGAATCC GATTGAAAAG CATTACAGCA AGCCGAATGT GACTGCTGTT 
GAAGTATTGC CAGTGTTTCC CGATTTCAAT CTGTGGAAAT ATCCATGTGC TCAGGTTATT 
TTTGATTCAG ATCCAGCGCC TGTTGGTAAA CAAGTGCCCG CGCAGATTGA GGAAATGTCA 
CAAGCTATGA TCAGAGGTGT GATGGACGAA CGTGGCGAAC AATTCGTCGC CTACTTCCTC 
CCAACTGAAG AGACTCTAGT TAAAAGACGG GAAGACTTTG CTAACGAGAT ACCATACCAA 
GACGATGAAG AATATGAGTA TAAAATGGCA AGAGAATACA ACTGGAACGT GAAGAGTAAA 
GCAAGCAAAG GTTACGAAGA AAATTACTTC TTCGTAATCA GACAAGATGG TGCCTACTAT 
AATGAGCTAG AAACCCGTGT CAGATTGAGT AAACGTCGCC AGAAAGTTGG CCAACCTGCC 
AGCAACACCC GTCTTATCGT CAAACACCGA CCAATGGATG CGAACGAATT CCGAATGCAA 
AGATACCGCG AAAAACAACT CGAACCTGCA GGCGATGAAG ACGAAGAAAT GGAAATGGAA 
CAAAACGAAG AACCACAAGA AAACGAAAAA GAAAGCGACG CGGAATCGTC AAGATCCAAA 
TCACCGTCAC GTTCAAGATC TCCGTCTAGA TCCCGATCAC AGTCTAAATC TCCCTCACGG 
TCCAGATCCA GATCCAAATC GCGATCAAGA TCCAGATCCA AATCGCGGTC AAGATCCAGA 
TCAAAATCTG CGTCTAGATC CAGATCACGT TCACGCTCGG CTTCCGGATC GCGATCTCCT 
TCCAAATCGC CGTCACGATC CAGATCCGGA TCCAAATCAC GATCACCATC ACGTTCCAAA 
TCACGGAGTA AATCACCGTC CAGATCCAGA TCCGGATCGC GGTCAAGATC CGGATCCCGA 
TCTGGATCCG AATCCGGCAG CAAGTCTGGA CAGTGA