LOC100254261 cDNA ORF clone, Vitis vinifera(wine grape)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100254261
Entrez Gene ID	100254261
Full Name	MAP3K epsilon protein kinase 1
Gene Type	protein-coding
Organism	Vitis vinifera(wine grape)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019226570.1	XP_019082115.1	MAP3K epsilon protein kinase 1 isoform X2
XM_010650037.2	XP_010648339.1	MAP3K epsilon protein kinase 1 isoform X3
XM_002277286.4	XP_002277322.2	MAP3K epsilon protein kinase 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100254261 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100254261 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OVa40160	XM_019226570.1 Latest version!	Vitis vinifera MAP3K epsilon protein kinase 1 (LOC100254261), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OVa28323	XM_010650037.2 Latest version!	Vitis vinifera MAP3K epsilon protein kinase 1 (LOC100254261), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OVa00542	XM_002277286.4 Latest version!	Vitis vinifera MAP3K epsilon protein kinase 1 (LOC100254261), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100254261 ( XM_019226570.1 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_019082115.1 Vitis vinifera MAP3K epsilon protein kinase 1 (LOC100254261), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OVa40160
Clone ID Related Accession (Same CDS sequence)	XM_019226570.1
Accession Version	XM_019226570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-21
Organism	Vitis vinifera(wine grape)
Product	MAP3K epsilon protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012007.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC AAGTGGCCAC GTCGCATTTC CACAAATCGA AGACTCTCGA CAACAAATAT 
ATGCTTGGAG ATGAGATCGG AAAAGGAGCA TATGGTCGAG TTTACAAGGG TTTGGATTTG 
GAGAATGGAG ACTTTGTTGC AATTAAACAA GTTTCTTTGG AGAATATTGC TCAAGAAGAT 
CTCAACATTA TCATGCAAGA GATTGATCTG CTCAAGAATT TGAACCACAA AAACATTGTG 
AAATATCTTG GATCTTTGAA GACAAGGAGT CATCTGCACA TAATTCTTGA GTATGTGGAG 
AATGGCTCAC TTGCAAACAT CATCAAACCA AATAAATTTG GGCCATTTCC AGAATCACTG 
GTGGCTGTTT ATATCTCTCA GGTCTTGGAG GGCTTGGTTT ATCTACATGA ACAAGGTGTC 
ATCCATCGGG ATATCAAGGG CGCAAATATA TTGACAACCA AAGAGGGTCT TGTGAAACTT 
GCTGACTTTG GTGTTGCAAC AAAATTAACT GAGGCAGATG TTAATACGCA TTCCGTTGTT 
GGGACACCAT ATTGGATGGC CCCTGAGGTA ATCGAAATGT CTGGGGTTTG TGCTGCTTCG 
GACATTTGGA GTGTTGGTTG CACTGTGATT GAGCTTCTTA CATGTGTACC TCCTTACTAT 
GATCTCCAGC CTATGCCAGC TCTATTCCGT ATTGTTCAGG ATGATCATCC TCCAATACCA 
GATGGCTTAT CTACTGACAT TACAGATTTT CTGCGGCAGT GTTTTAAGAA GGATGCTAGG 
CAGAGGCCTG ATGCAAAGAC ACTGCTTTCA CATCCTTGGA TACGGAACTG TAGGCGTGCT 
TTGCAGTCCT CTCTTCGTCA TAGTGGAACA TTGAGAAATA TACAGGAAGA TGCTTCAGTT 
GATGCAGAGA TTTCCAATGG AGATGATCAA AGTATTGGTG AAAGCCCTTC TGATGAGAAA 
GCAGAAGTAA TTGCCTCTGA ATTTGAAAAT GATTCCAGAA AAGAGTGTTT ACCAACTGAG 
GTTGTTGATA CCGGCAAATC TTATACGGAT TCCAATGGAG ATCTTATTGA GGATGAAGTT 
GATAATCCAG AAGAGTATGT TCAATCAGAT CAAGTACCCA CATTGGCCAT TCACGAAAAA 
TCATCTCTAC CAACTACTTC TGGTATATTG CCTGCTAATA AGGATGTGGC TCCCCCTAGT 
CCAACAGATT CTAACGAGGT ACTAGATATG GGTGATCAGG ATGAAGCACT AATGAATGGT 
AAGGTGGGAT CTCCTAAGTC AAGAAAGAAC AATGTTTTAT ATAAAAAGAG TGAAGGGAAA 
GGAAGTTCTA CTCAACTTGA CAACAGATTA TTTGGTTTTG TACCAAGAAG TCAGGAGAAT 
AGTTCTCGAA AGGCTGCAAA GGCACCAGTC ATTTCGGGCG GAAATGAGCT AAGTAAATTT 
AGTGACACGC CAGGAGATGC TTCTTTGGAA GATTTATTTC ATCCACTGCA TAAGAATCCT 
GAGGATCAGG CAGCTGAAGC TTCAACATCT GCATCCTCAT CACATGTTGT ACAAGGCAAT 
GCATTCATAA ATGATGCTGG AAAAAATGAC CTGGCAACAA AGTTGAGGGC TACAATTGCT 
CAGAAACAAA TGGAGAATGA GATTGGGCAA ACAAATGGAG ATCTCTTCAG TTTAATGTTG 
GATGTTCTGA AGGAGGACGT GATGGATATT GATGGTTTGG TTTTTGATGA CAAAATGCCT 
GGAGAGAACC TTTTTCCCCT ACAGGCTGTT GAGTTTAGCA GATTAGTTGG GTCATTAAGA 
CCACAGGAAC CAGAAGATGT GATCGTGTCT GCCTGTTTGA AGCTTATATC CATTTTTCAC 
CAACGCCCTG AGCAGAAAGG TGTGTTTGTT ACTCAGCATG GTTTACTCCC CCTCATGGAA 
CTACTTGAAG TTTCTAGAAC TCGTGTTATA TGTTCAGTGC TTCAAATTGT AAATCAGATA 
ATCAAGGATA ATACCGATTT CCAGGAAAAT GCTTGTCTTG TTGGCCTGAT CCCTGTTGTT 
ATGAGCTTTG CAGTACCTGA TCATCCCCGG GAAGTTCGCA TGGAAGCCGC TTATTTCTCG 
CAGCAGCTTT GTCAGTCTAG CTCCTTGACA TTGCAAATGT TCATTGCTTG TGGTGGAATA 
CCTGTTTTAG TGGGCTTTCT GGAGGCTGAT TATGTGAGGG AAATGGTTCA TCTAGCTATT 
GATGGCATGT GGCAGGTCTT CAAGCTTCAG CGATCAACAC CTAGAAATGA TTTCTGTCGC 
ATTGCTGCAA AGAATGGAAT ACTTCCTAGG CTTATAAACA CTCTTTATAG TTTGAATGAG 
GCTGCTCGTC TAGCTTCCAT AGCTGGTGGT AGTGGATTTA CAATAGAAGG TTTGGCTCCG 
CGACCACGTT CTGGTTCACT GGATCCCAGC TCTCCTATTT TTATTCAGGG TGAGATATCA 
CTTACTGGAA TCGATCATCC TGATCTCCTT AAGGTTAGGC ATGGGCTAAT TGATCATTCC 
TTGTCAACTG CAACCCAAGA ACCTTCACGT GTTTCAGCAT CACATCCCCA AAGGTCAGAT 
GCTAACCAAC CAGATTCCCG ATACTTCTCT CTGGATACTG ACAGGCCTGC AATGGAAGCT 
TCAGTTGCTT CTAAATTACA AGACCTTGCA TTTTCAGAAA AAGTTGCAAA TATGCAAACA 
AAGGAATCTT CTGGAACTAT TTTGAAAGAG CGAGAAAACT TGGACCGCTG GAAAATTGAT 
CCACAACGAG TCCCTAATTC GGCTAATAGG ACATCAGTAG ACAGACCTTC AAAATTGGTG 
GAAGGTGTGT CAAATGGGTT TCCTAGTACA ATAGGGACCC AGCAAGAGCA AGTTCGACCT 
CTTCTTAGCT TGTTGGATAA GGAGCCCCCT TCCCGGCATT TTTCTGGTCA GCTAGAGTAT 
GTACGGCACC TCTCTGGGTT GGAGAGACAT GAAAGCATAC TGCCTCTACT ACATGCAACC 
AATGAGAAGA AAACAAATGG TGAACTAGAT TTTCTGATGG CAGAATTTGC AGAGGTTTCT 
GGTCGTGGAA GAGAAAATGG AAATCTGGAT TCTGCACCTA GAATTTCAAA TAAAACTGTA 
AATAAGAAGA TTCCATTGGC ATCTAATGAG GGTGCTGCTT CTACATCTGG GATTGCTTCT 
CAGACAGCAT CAGGTGTACT GTCTGGTTCT GGTGTTTTAA ATGCTAGGCC AGGAAGTGCA 
ACGTCATCTG GGTTGCTTTC CCATATGGTA TCATCACTCA ATGCTGATGT TGCTAAGGAA 
TATCTAGAAA AGGTTGCAGA CCTTCTGCTG GAGTTTGCCC AAGCAGATAC GACAGTAAAA 
TCCTACATGT GTAGCCAAAG CTTGCTTAGC CGTCTTTTTC AGATGTTCAA TCGGATAGAG 
CCGCCTATTC TTTTGAAGAT ACTAAAGTGT ATCAATCATT TGTCAACCGA CCCAAATTGC 
TTAGAAAATC TTCAGCGTGC CGATGCAATC AAATACTTGA TCCCAAACCT TGAACTCAAA 
GAAGGGCCTC TTGTATTTCA AATACATTAT GAGGTCCTCT ATGCATTATT CAACCTCTGC 
AAGATAAATA AGAGGAGGCA AGAACAAGCA GCTGAAAATG GAATCATTCC ACACTTGATG 
CATTTTATAA TGTCAGATTC TCCCTTGAAA CAACATGCAT TGCCTCTACT GTGCGATATG 
GCCCATGCAT CACGTAACTC AAGGGAGCAA TTAAGGGCCC ATGATGGGTT GGATGTGTAC 
TTAAGCCTAC TTGAGGATGA GCTTTGGTCT GTGACAGCAT TAGATTCTAT TGCTGTGTGC 
TTGGCCCATG ACAATGACAA CCGGAAAGTA GAACAGGCGT TGCTGAAAAA GGATGCAATT 
CAGAAATTGG TCAAATTCTT CCAGTGCTGT CCTGAACAAC ATTTTGTGCA CATATTGGAG 
CCCTTCTTGA AGATTATCAC GAAATCATCC CGGATAAATA CAACACTAGC TATTAATGGG 
TTGACACCCT TGCTTATTGC AAGGCTTGAC CATCAGGATG CTATAGCTCG TCTTAATCTG 
CTCAAACTAA TAAAGTCTGT TTATGAGCAC CATCCACGGC CAAAGCAACT GATCGTGGAG 
AATGATCTGC CTCAGAAGCT TCAGAATCTG ATTGAGGAGC GCAGAGATGG GCAACGTTCT 
GGAGGCCAAG TGTTGGTTAA ACAGATGGCT ACTTCTCTCC TTAAAGCACT CCATATCAAC 
ACCGTTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019082115.1
CDS	270..4541
Translation	MSRQVATSHFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNIIMQEIDLLKNLNHKNIVKYLGSLKTRSHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQDDHPPIPDGLSTDITDFLRQCFKKDARQRPDAKTLLSHPWIRNCRRALQSSLRHSGTLRNIQEDASVDAEISNGDDQSIGESPSDEKAEVIASEFENDSRKECLPTEVVDTGKSYTDSNGDLIEDEVDNPEEYVQSDQVPTLAIHEKSSLPTTSGILPANKDVAPPSPTDSNEVLDMGDQDEALMNGKVGSPKSRKNNVLYKKSEGKGSSTQLDNRLFGFVPRSQENSSRKAAKAPVISGGNELSKFSDTPGDASLEDLFHPLHKNPEDQAAEASTSASSSHVVQGNAFINDAGKNDLATKLRATIAQKQMENEIGQTNGDLFSLMLDVLKEDVMDIDGLVFDDKMPGENLFPLQAVEFSRLVGSLRPQEPEDVIVSACLKLISIFHQRPEQKGVFVTQHGLLPLMELLEVSRTRVICSVLQIVNQIIKDNTDFQENACLVGLIPVVMSFAVPDHPREVRMEAAYFSQQLCQSSSLTLQMFIACGGIPVLVGFLEADYVREMVHLAIDGMWQVFKLQRSTPRNDFCRIAAKNGILPRLINTLYSLNEAARLASIAGGSGFTIEGLAPRPRSGSLDPSSPIFIQGEISLTGIDHPDLLKVRHGLIDHSLSTATQEPSRVSASHPQRSDANQPDSRYFSLDTDRPAMEASVASKLQDLAFSEKVANMQTKESSGTILKERENLDRWKIDPQRVPNSANRTSVDRPSKLVEGVSNGFPSTIGTQQEQVRPLLSLLDKEPPSRHFSGQLEYVRHLSGLERHESILPLLHATNEKKTNGELDFLMAEFAEVSGRGRENGNLDSAPRISNKTVNKKIPLASNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSSLNADVAKEYLEKVADLLLEFAQADTTVKSYMCSQSLLSRLFQMFNRIEPPILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGPLVFQIHYEVLYALFNLCKINKRRQEQAAENGIIPHLMHFIMSDSPLKQHALPLLCDMAHASRNSREQLRAHDGLDVYLSLLEDELWSVTALDSIAVCLAHDNDNRKVEQALLKKDAIQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTLAINGLTPLLIARLDHQDAIARLNLLKLIKSVYEHHPRPKQLIVENDLPQKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL

Target ORF information:

RefSeq Version	XM_019226570.1
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera MAP3K epsilon protein kinase 1 (LOC100254261), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019226570.1

ORF Insert Sequence:

ATGTCTCGCC AAGTGGCCAC GTCGCATTTC CACAAATCGA AGACTCTCGA CAACAAATAT 
ATGCTTGGAG ATGAGATCGG AAAAGGAGCA TATGGTCGAG TTTACAAGGG TTTGGATTTG 
GAGAATGGAG ACTTTGTTGC AATTAAACAA GTTTCTTTGG AGAATATTGC TCAAGAAGAT 
CTCAACATTA TCATGCAAGA GATTGATCTG CTCAAGAATT TGAACCACAA AAACATTGTG 
AAATATCTTG GATCTTTGAA GACAAGGAGT CATCTGCACA TAATTCTTGA GTATGTGGAG 
AATGGCTCAC TTGCAAACAT CATCAAACCA AATAAATTTG GGCCATTTCC AGAATCACTG 
GTGGCTGTTT ATATCTCTCA GGTCTTGGAG GGCTTGGTTT ATCTACATGA ACAAGGTGTC 
ATCCATCGGG ATATCAAGGG CGCAAATATA TTGACAACCA AAGAGGGTCT TGTGAAACTT 
GCTGACTTTG GTGTTGCAAC AAAATTAACT GAGGCAGATG TTAATACGCA TTCCGTTGTT 
GGGACACCAT ATTGGATGGC CCCTGAGGTA ATCGAAATGT CTGGGGTTTG TGCTGCTTCG 
GACATTTGGA GTGTTGGTTG CACTGTGATT GAGCTTCTTA CATGTGTACC TCCTTACTAT 
GATCTCCAGC CTATGCCAGC TCTATTCCGT ATTGTTCAGG ATGATCATCC TCCAATACCA 
GATGGCTTAT CTACTGACAT TACAGATTTT CTGCGGCAGT GTTTTAAGAA GGATGCTAGG 
CAGAGGCCTG ATGCAAAGAC ACTGCTTTCA CATCCTTGGA TACGGAACTG TAGGCGTGCT 
TTGCAGTCCT CTCTTCGTCA TAGTGGAACA TTGAGAAATA TACAGGAAGA TGCTTCAGTT 
GATGCAGAGA TTTCCAATGG AGATGATCAA AGTATTGGTG AAAGCCCTTC TGATGAGAAA 
GCAGAAGTAA TTGCCTCTGA ATTTGAAAAT GATTCCAGAA AAGAGTGTTT ACCAACTGAG 
GTTGTTGATA CCGGCAAATC TTATACGGAT TCCAATGGAG ATCTTATTGA GGATGAAGTT 
GATAATCCAG AAGAGTATGT TCAATCAGAT CAAGTACCCA CATTGGCCAT TCACGAAAAA 
TCATCTCTAC CAACTACTTC TGGTATATTG CCTGCTAATA AGGATGTGGC TCCCCCTAGT 
CCAACAGATT CTAACGAGGT ACTAGATATG GGTGATCAGG ATGAAGCACT AATGAATGGT 
AAGGTGGGAT CTCCTAAGTC AAGAAAGAAC AATGTTTTAT ATAAAAAGAG TGAAGGGAAA 
GGAAGTTCTA CTCAACTTGA CAACAGATTA TTTGGTTTTG TACCAAGAAG TCAGGAGAAT 
AGTTCTCGAA AGGCTGCAAA GGCACCAGTC ATTTCGGGCG GAAATGAGCT AAGTAAATTT 
AGTGACACGC CAGGAGATGC TTCTTTGGAA GATTTATTTC ATCCACTGCA TAAGAATCCT 
GAGGATCAGG CAGCTGAAGC TTCAACATCT GCATCCTCAT CACATGTTGT ACAAGGCAAT 
GCATTCATAA ATGATGCTGG AAAAAATGAC CTGGCAACAA AGTTGAGGGC TACAATTGCT 
CAGAAACAAA TGGAGAATGA GATTGGGCAA ACAAATGGAG ATCTCTTCAG TTTAATGTTG 
GATGTTCTGA AGGAGGACGT GATGGATATT GATGGTTTGG TTTTTGATGA CAAAATGCCT 
GGAGAGAACC TTTTTCCCCT ACAGGCTGTT GAGTTTAGCA GATTAGTTGG GTCATTAAGA 
CCACAGGAAC CAGAAGATGT GATCGTGTCT GCCTGTTTGA AGCTTATATC CATTTTTCAC 
CAACGCCCTG AGCAGAAAGG TGTGTTTGTT ACTCAGCATG GTTTACTCCC CCTCATGGAA 
CTACTTGAAG TTTCTAGAAC TCGTGTTATA TGTTCAGTGC TTCAAATTGT AAATCAGATA 
ATCAAGGATA ATACCGATTT CCAGGAAAAT GCTTGTCTTG TTGGCCTGAT CCCTGTTGTT 
ATGAGCTTTG CAGTACCTGA TCATCCCCGG GAAGTTCGCA TGGAAGCCGC TTATTTCTCG 
CAGCAGCTTT GTCAGTCTAG CTCCTTGACA TTGCAAATGT TCATTGCTTG TGGTGGAATA 
CCTGTTTTAG TGGGCTTTCT GGAGGCTGAT TATGTGAGGG AAATGGTTCA TCTAGCTATT 
GATGGCATGT GGCAGGTCTT CAAGCTTCAG CGATCAACAC CTAGAAATGA TTTCTGTCGC 
ATTGCTGCAA AGAATGGAAT ACTTCCTAGG CTTATAAACA CTCTTTATAG TTTGAATGAG 
GCTGCTCGTC TAGCTTCCAT AGCTGGTGGT AGTGGATTTA CAATAGAAGG TTTGGCTCCG 
CGACCACGTT CTGGTTCACT GGATCCCAGC TCTCCTATTT TTATTCAGGG TGAGATATCA 
CTTACTGGAA TCGATCATCC TGATCTCCTT AAGGTTAGGC ATGGGCTAAT TGATCATTCC 
TTGTCAACTG CAACCCAAGA ACCTTCACGT GTTTCAGCAT CACATCCCCA AAGGTCAGAT 
GCTAACCAAC CAGATTCCCG ATACTTCTCT CTGGATACTG ACAGGCCTGC AATGGAAGCT 
TCAGTTGCTT CTAAATTACA AGACCTTGCA TTTTCAGAAA AAGTTGCAAA TATGCAAACA 
AAGGAATCTT CTGGAACTAT TTTGAAAGAG CGAGAAAACT TGGACCGCTG GAAAATTGAT 
CCACAACGAG TCCCTAATTC GGCTAATAGG ACATCAGTAG ACAGACCTTC AAAATTGGTG 
GAAGGTGTGT CAAATGGGTT TCCTAGTACA ATAGGGACCC AGCAAGAGCA AGTTCGACCT 
CTTCTTAGCT TGTTGGATAA GGAGCCCCCT TCCCGGCATT TTTCTGGTCA GCTAGAGTAT 
GTACGGCACC TCTCTGGGTT GGAGAGACAT GAAAGCATAC TGCCTCTACT ACATGCAACC 
AATGAGAAGA AAACAAATGG TGAACTAGAT TTTCTGATGG CAGAATTTGC AGAGGTTTCT 
GGTCGTGGAA GAGAAAATGG AAATCTGGAT TCTGCACCTA GAATTTCAAA TAAAACTGTA 
AATAAGAAGA TTCCATTGGC ATCTAATGAG GGTGCTGCTT CTACATCTGG GATTGCTTCT 
CAGACAGCAT CAGGTGTACT GTCTGGTTCT GGTGTTTTAA ATGCTAGGCC AGGAAGTGCA 
ACGTCATCTG GGTTGCTTTC CCATATGGTA TCATCACTCA ATGCTGATGT TGCTAAGGAA 
TATCTAGAAA AGGTTGCAGA CCTTCTGCTG GAGTTTGCCC AAGCAGATAC GACAGTAAAA 
TCCTACATGT GTAGCCAAAG CTTGCTTAGC CGTCTTTTTC AGATGTTCAA TCGGATAGAG 
CCGCCTATTC TTTTGAAGAT ACTAAAGTGT ATCAATCATT TGTCAACCGA CCCAAATTGC 
TTAGAAAATC TTCAGCGTGC CGATGCAATC AAATACTTGA TCCCAAACCT TGAACTCAAA 
GAAGGGCCTC TTGTATTTCA AATACATTAT GAGGTCCTCT ATGCATTATT CAACCTCTGC 
AAGATAAATA AGAGGAGGCA AGAACAAGCA GCTGAAAATG GAATCATTCC ACACTTGATG 
CATTTTATAA TGTCAGATTC TCCCTTGAAA CAACATGCAT TGCCTCTACT GTGCGATATG 
GCCCATGCAT CACGTAACTC AAGGGAGCAA TTAAGGGCCC ATGATGGGTT GGATGTGTAC 
TTAAGCCTAC TTGAGGATGA GCTTTGGTCT GTGACAGCAT TAGATTCTAT TGCTGTGTGC 
TTGGCCCATG ACAATGACAA CCGGAAAGTA GAACAGGCGT TGCTGAAAAA GGATGCAATT 
CAGAAATTGG TCAAATTCTT CCAGTGCTGT CCTGAACAAC ATTTTGTGCA CATATTGGAG 
CCCTTCTTGA AGATTATCAC GAAATCATCC CGGATAAATA CAACACTAGC TATTAATGGG 
TTGACACCCT TGCTTATTGC AAGGCTTGAC CATCAGGATG CTATAGCTCG TCTTAATCTG 
CTCAAACTAA TAAAGTCTGT TTATGAGCAC CATCCACGGC CAAAGCAACT GATCGTGGAG 
AATGATCTGC CTCAGAAGCT TCAGAATCTG ATTGAGGAGC GCAGAGATGG GCAACGTTCT 
GGAGGCCAAG TGTTGGTTAA ACAGATGGCT ACTTCTCTCC TTAAAGCACT CCATATCAAC 
ACCGTTTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100254261 ( XM_010650037.2 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_010648339.1 Vitis vinifera MAP3K epsilon protein kinase 1 (LOC100254261), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OVa28323
Clone ID Related Accession (Same CDS sequence)	XM_010650037.2
Accession Version	XM_010650037.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-21
Organism	Vitis vinifera(wine grape)
Product	MAP3K epsilon protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012007.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 22, 2016 this sequence version replaced XM_010650037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGATG ATATGTTAGC AAAACGGGGA GCAAAGCAGT TGATTCCTTT TGTAGGAAAT 
ATACAGGAAG ATGCTTCAGT TGATGCAGAG ATTTCCAATG GAGATGATCA AAGTATTGGT 
GAAAGCCCTT CTGATGAGAA AGCAGAAGTA ATTGCCTCTG AATTTGAAAA TGATTCCAGA 
AAAGAGTGTT TACCAACTGA GGTTGTTGAT ACCGGCAAAT CTTATACGGA TTCCAATGGA 
GATCTTATTG AGGATGAAGT TGATAATCCA GAAGAGTATG TTCAATCAGA TCAAGTACCC 
ACATTGGCCA TTCACGAAAA ATCATCTCTA CCAACTACTT CTGGTATATT GCCTGCTAAT 
AAGGATGTGG CTCCCCCTAG TCCAACAGAT TCTAACGAGG TACTAGATAT GGGTGATCAG 
GATGAAGCAC TAATGAATGG TAAGGTGGGA TCTCCTAAGT CAAGAAAGAA CAATGTTTTA 
TATAAAAAGA GTGAAGGGAA AGGAAGTTCT ACTCAACTTG ACAACAGATT ATTTGGTTTT 
GTACCAAGAA GTCAGGAGAA TAGTTCTCGA AAGGCTGCAA AGGCACCAGT CATTTCGGGC 
GGAAATGAGC TAAGTAAATT TAGTGACACG CCAGGAGATG CTTCTTTGGA AGATTTATTT 
CATCCACTGC ATAAGAATCC TGAGGATCAG GCAGCTGAAG CTTCAACATC TGCATCCTCA 
TCACATGTTG TACAAGGCAA TGCATTCATA AATGATGCTG GAAAAAATGA CCTGGCAACA 
AAGTTGAGGG CTACAATTGC TCAGAAACAA ATGGAGAATG AGATTGGGCA AACAAATGGA 
GATCTCTTCA GTTTAATGTT GGATGTTCTG AAGGAGGACG TGATGGATAT TGATGGTTTG 
GTTTTTGATG ACAAAATGCC TGGAGAGAAC CTTTTTCCCC TACAGGCTGT TGAGTTTAGC 
AGATTAGTTG GGTCATTAAG ACCACAGGAA CCAGAAGATG TGATCGTGTC TGCCTGTTTG 
AAGCTTATAT CCATTTTTCA CCAACGCCCT GAGCAGAAAG GTGTGTTTGT TACTCAGCAT 
GGTTTACTCC CCCTCATGGA ACTACTTGAA GTTTCTAGAA CTCGTGTTAT ATGTTCAGTG 
CTTCAAATTG TAAATCAGAT AATCAAGGAT AATACCGATT TCCAGGAAAA TGCTTGTCTT 
GTTGGCCTGA TCCCTGTTGT TATGAGCTTT GCAGTACCTG ATCATCCCCG GGAAGTTCGC 
ATGGAAGCCG CTTATTTCTC GCAGCAGCTT TGTCAGTCTA GCTCCTTGAC ATTGCAAATG 
TTCATTGCTT GTGGTGGAAT ACCTGTTTTA GTGGGCTTTC TGGAGGCTGA TTATGTGAGG 
TACAGGGAAA TGGTTCATCT AGCTATTGAT GGCATGTGGC AGGTCTTCAA GCTTCAGCGA 
TCAACACCTA GAAATGATTT CTGTCGCATT GCTGCAAAGA ATGGAATACT TCCTAGGCTT 
ATAAACACTC TTTATAGTTT GAATGAGGCT GCTCGTCTAG CTTCCATAGC TGGTGGTAGT 
GGATTTACAA TAGAAGGTTT GGCTCCGCGA CCACGTTCTG GTTCACTGGA TCCCAGCTCT 
CCTATTTTTA TTCAGGGTGA GATATCACTT ACTGGAATCG ATCATCCTGA TCTCCTTAAG 
GTTAGGCATG GGCTAATTGA TCATTCCTTG TCAACTGCAA CCCAAGAACC TTCACGTGTT 
TCAGCATCAC ATCCCCAAAG GTCAGATGCT AACCAACCAG ATTCCCGATA CTTCTCTCTG 
GATACTGACA GGCCTGCAAT GGAAGCTTCA GTTGCTTCTA AATTACAAGA CCTTGCATTT 
TCAGAAAAAG TTGCAAATAT GCAAACAAAG GAATCTTCTG GAACTATTTT GAAAGAGCGA 
GAAAACTTGG ACCGCTGGAA AATTGATCCA CAACGAGTCC CTAATTCGGC TAATAGGACA 
TCAGTAGACA GACCTTCAAA ATTGGTGGAA GGTGTGTCAA ATGGGTTTCC TAGTACAATA 
GGGACCCAGC AAGAGCAAGT TCGACCTCTT CTTAGCTTGT TGGATAAGGA GCCCCCTTCC 
CGGCATTTTT CTGGTCAGCT AGAGTATGTA CGGCACCTCT CTGGGTTGGA GAGACATGAA 
AGCATACTGC CTCTACTACA TGCAACCAAT GAGAAGAAAA CAAATGGTGA ACTAGATTTT 
CTGATGGCAG AATTTGCAGA GGTTTCTGGT CGTGGAAGAG AAAATGGAAA TCTGGATTCT 
GCACCTAGAA TTTCAAATAA AACTGTAAAT AAGAAGATTC CATTGGCATC TAATGAGGGT 
GCTGCTTCTA CATCTGGGAT TGCTTCTCAG ACAGCATCAG GTGTACTGTC TGGTTCTGGT 
GTTTTAAATG CTAGGCCAGG AAGTGCAACG TCATCTGGGT TGCTTTCCCA TATGGTATCA 
TCACTCAATG CTGATGTTGC TAAGGAATAT CTAGAAAAGG TTGCAGACCT TCTGCTGGAG 
TTTGCCCAAG CAGATACGAC AGTAAAATCC TACATGTGTA GCCAAAGCTT GCTTAGCCGT 
CTTTTTCAGA TGTTCAATCG GATAGAGCCG CCTATTCTTT TGAAGATACT AAAGTGTATC 
AATCATTTGT CAACCGACCC AAATTGCTTA GAAAATCTTC AGCGTGCCGA TGCAATCAAA 
TACTTGATCC CAAACCTTGA ACTCAAAGAA GGGCCTCTTG TATTTCAAAT ACATTATGAG 
GTCCTCTATG CATTATTCAA CCTCTGCAAG ATAAATAAGA GGAGGCAAGA ACAAGCAGCT 
GAAAATGGAA TCATTCCACA CTTGATGCAT TTTATAATGT CAGATTCTCC CTTGAAACAA 
CATGCATTGC CTCTACTGTG CGATATGGCC CATGCATCAC GTAACTCAAG GGAGCAATTA 
AGGGCCCATG ATGGGTTGGA TGTGTACTTA AGCCTACTTG AGGATGAGCT TTGGTCTGTG 
ACAGCATTAG ATTCTATTGC TGTGTGCTTG GCCCATGACA ATGACAACCG GAAAGTAGAA 
CAGGCGTTGC TGAAAAAGGA TGCAATTCAG AAATTGGTCA AATTCTTCCA GTGCTGTCCT 
GAACAACATT TTGTGCACAT ATTGGAGCCC TTCTTGAAGA TTATCACGAA ATCATCCCGG 
ATAAATACAA CACTAGCTAT TAATGGGTTG ACACCCTTGC TTATTGCAAG GCTTGACCAT 
CAGGATGCTA TAGCTCGTCT TAATCTGCTC AAACTAATAA AGTCTGTTTA TGAGCACCAT 
CCACGGCCAA AGCAACTGAT CGTGGAGAAT GATCTGCCTC AGAAGCTTCA GAATCTGATT 
GAGGAGCGCA GAGATGGGCA ACGTTCTGGA GGCCAAGTGT TGGTTAAACA GATGGCTACT 
TCTCTCCTTA AAGCACTCCA TATCAACACC GTTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010648339.1
CDS	59..3517
Translation	MPDDMLAKRGAKQLIPFVGNIQEDASVDAEISNGDDQSIGESPSDEKAEVIASEFENDSRKECLPTEVVDTGKSYTDSNGDLIEDEVDNPEEYVQSDQVPTLAIHEKSSLPTTSGILPANKDVAPPSPTDSNEVLDMGDQDEALMNGKVGSPKSRKNNVLYKKSEGKGSSTQLDNRLFGFVPRSQENSSRKAAKAPVISGGNELSKFSDTPGDASLEDLFHPLHKNPEDQAAEASTSASSSHVVQGNAFINDAGKNDLATKLRATIAQKQMENEIGQTNGDLFSLMLDVLKEDVMDIDGLVFDDKMPGENLFPLQAVEFSRLVGSLRPQEPEDVIVSACLKLISIFHQRPEQKGVFVTQHGLLPLMELLEVSRTRVICSVLQIVNQIIKDNTDFQENACLVGLIPVVMSFAVPDHPREVRMEAAYFSQQLCQSSSLTLQMFIACGGIPVLVGFLEADYVRYREMVHLAIDGMWQVFKLQRSTPRNDFCRIAAKNGILPRLINTLYSLNEAARLASIAGGSGFTIEGLAPRPRSGSLDPSSPIFIQGEISLTGIDHPDLLKVRHGLIDHSLSTATQEPSRVSASHPQRSDANQPDSRYFSLDTDRPAMEASVASKLQDLAFSEKVANMQTKESSGTILKERENLDRWKIDPQRVPNSANRTSVDRPSKLVEGVSNGFPSTIGTQQEQVRPLLSLLDKEPPSRHFSGQLEYVRHLSGLERHESILPLLHATNEKKTNGELDFLMAEFAEVSGRGRENGNLDSAPRISNKTVNKKIPLASNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSSLNADVAKEYLEKVADLLLEFAQADTTVKSYMCSQSLLSRLFQMFNRIEPPILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGPLVFQIHYEVLYALFNLCKINKRRQEQAAENGIIPHLMHFIMSDSPLKQHALPLLCDMAHASRNSREQLRAHDGLDVYLSLLEDELWSVTALDSIAVCLAHDNDNRKVEQALLKKDAIQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTLAINGLTPLLIARLDHQDAIARLNLLKLIKSVYEHHPRPKQLIVENDLPQKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL

Target ORF information:

RefSeq Version	XM_010650037.2
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera MAP3K epsilon protein kinase 1 (LOC100254261), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010650037.2

ORF Insert Sequence:

ATGCCAGATG ATATGTTAGC AAAACGGGGA GCAAAGCAGT TGATTCCTTT TGTAGGAAAT 
ATACAGGAAG ATGCTTCAGT TGATGCAGAG ATTTCCAATG GAGATGATCA AAGTATTGGT 
GAAAGCCCTT CTGATGAGAA AGCAGAAGTA ATTGCCTCTG AATTTGAAAA TGATTCCAGA 
AAAGAGTGTT TACCAACTGA GGTTGTTGAT ACCGGCAAAT CTTATACGGA TTCCAATGGA 
GATCTTATTG AGGATGAAGT TGATAATCCA GAAGAGTATG TTCAATCAGA TCAAGTACCC 
ACATTGGCCA TTCACGAAAA ATCATCTCTA CCAACTACTT CTGGTATATT GCCTGCTAAT 
AAGGATGTGG CTCCCCCTAG TCCAACAGAT TCTAACGAGG TACTAGATAT GGGTGATCAG 
GATGAAGCAC TAATGAATGG TAAGGTGGGA TCTCCTAAGT CAAGAAAGAA CAATGTTTTA 
TATAAAAAGA GTGAAGGGAA AGGAAGTTCT ACTCAACTTG ACAACAGATT ATTTGGTTTT 
GTACCAAGAA GTCAGGAGAA TAGTTCTCGA AAGGCTGCAA AGGCACCAGT CATTTCGGGC 
GGAAATGAGC TAAGTAAATT TAGTGACACG CCAGGAGATG CTTCTTTGGA AGATTTATTT 
CATCCACTGC ATAAGAATCC TGAGGATCAG GCAGCTGAAG CTTCAACATC TGCATCCTCA 
TCACATGTTG TACAAGGCAA TGCATTCATA AATGATGCTG GAAAAAATGA CCTGGCAACA 
AAGTTGAGGG CTACAATTGC TCAGAAACAA ATGGAGAATG AGATTGGGCA AACAAATGGA 
GATCTCTTCA GTTTAATGTT GGATGTTCTG AAGGAGGACG TGATGGATAT TGATGGTTTG 
GTTTTTGATG ACAAAATGCC TGGAGAGAAC CTTTTTCCCC TACAGGCTGT TGAGTTTAGC 
AGATTAGTTG GGTCATTAAG ACCACAGGAA CCAGAAGATG TGATCGTGTC TGCCTGTTTG 
AAGCTTATAT CCATTTTTCA CCAACGCCCT GAGCAGAAAG GTGTGTTTGT TACTCAGCAT 
GGTTTACTCC CCCTCATGGA ACTACTTGAA GTTTCTAGAA CTCGTGTTAT ATGTTCAGTG 
CTTCAAATTG TAAATCAGAT AATCAAGGAT AATACCGATT TCCAGGAAAA TGCTTGTCTT 
GTTGGCCTGA TCCCTGTTGT TATGAGCTTT GCAGTACCTG ATCATCCCCG GGAAGTTCGC 
ATGGAAGCCG CTTATTTCTC GCAGCAGCTT TGTCAGTCTA GCTCCTTGAC ATTGCAAATG 
TTCATTGCTT GTGGTGGAAT ACCTGTTTTA GTGGGCTTTC TGGAGGCTGA TTATGTGAGG 
TACAGGGAAA TGGTTCATCT AGCTATTGAT GGCATGTGGC AGGTCTTCAA GCTTCAGCGA 
TCAACACCTA GAAATGATTT CTGTCGCATT GCTGCAAAGA ATGGAATACT TCCTAGGCTT 
ATAAACACTC TTTATAGTTT GAATGAGGCT GCTCGTCTAG CTTCCATAGC TGGTGGTAGT 
GGATTTACAA TAGAAGGTTT GGCTCCGCGA CCACGTTCTG GTTCACTGGA TCCCAGCTCT 
CCTATTTTTA TTCAGGGTGA GATATCACTT ACTGGAATCG ATCATCCTGA TCTCCTTAAG 
GTTAGGCATG GGCTAATTGA TCATTCCTTG TCAACTGCAA CCCAAGAACC TTCACGTGTT 
TCAGCATCAC ATCCCCAAAG GTCAGATGCT AACCAACCAG ATTCCCGATA CTTCTCTCTG 
GATACTGACA GGCCTGCAAT GGAAGCTTCA GTTGCTTCTA AATTACAAGA CCTTGCATTT 
TCAGAAAAAG TTGCAAATAT GCAAACAAAG GAATCTTCTG GAACTATTTT GAAAGAGCGA 
GAAAACTTGG ACCGCTGGAA AATTGATCCA CAACGAGTCC CTAATTCGGC TAATAGGACA 
TCAGTAGACA GACCTTCAAA ATTGGTGGAA GGTGTGTCAA ATGGGTTTCC TAGTACAATA 
GGGACCCAGC AAGAGCAAGT TCGACCTCTT CTTAGCTTGT TGGATAAGGA GCCCCCTTCC 
CGGCATTTTT CTGGTCAGCT AGAGTATGTA CGGCACCTCT CTGGGTTGGA GAGACATGAA 
AGCATACTGC CTCTACTACA TGCAACCAAT GAGAAGAAAA CAAATGGTGA ACTAGATTTT 
CTGATGGCAG AATTTGCAGA GGTTTCTGGT CGTGGAAGAG AAAATGGAAA TCTGGATTCT 
GCACCTAGAA TTTCAAATAA AACTGTAAAT AAGAAGATTC CATTGGCATC TAATGAGGGT 
GCTGCTTCTA CATCTGGGAT TGCTTCTCAG ACAGCATCAG GTGTACTGTC TGGTTCTGGT 
GTTTTAAATG CTAGGCCAGG AAGTGCAACG TCATCTGGGT TGCTTTCCCA TATGGTATCA 
TCACTCAATG CTGATGTTGC TAAGGAATAT CTAGAAAAGG TTGCAGACCT TCTGCTGGAG 
TTTGCCCAAG CAGATACGAC AGTAAAATCC TACATGTGTA GCCAAAGCTT GCTTAGCCGT 
CTTTTTCAGA TGTTCAATCG GATAGAGCCG CCTATTCTTT TGAAGATACT AAAGTGTATC 
AATCATTTGT CAACCGACCC AAATTGCTTA GAAAATCTTC AGCGTGCCGA TGCAATCAAA 
TACTTGATCC CAAACCTTGA ACTCAAAGAA GGGCCTCTTG TATTTCAAAT ACATTATGAG 
GTCCTCTATG CATTATTCAA CCTCTGCAAG ATAAATAAGA GGAGGCAAGA ACAAGCAGCT 
GAAAATGGAA TCATTCCACA CTTGATGCAT TTTATAATGT CAGATTCTCC CTTGAAACAA 
CATGCATTGC CTCTACTGTG CGATATGGCC CATGCATCAC GTAACTCAAG GGAGCAATTA 
AGGGCCCATG ATGGGTTGGA TGTGTACTTA AGCCTACTTG AGGATGAGCT TTGGTCTGTG 
ACAGCATTAG ATTCTATTGC TGTGTGCTTG GCCCATGACA ATGACAACCG GAAAGTAGAA 
CAGGCGTTGC TGAAAAAGGA TGCAATTCAG AAATTGGTCA AATTCTTCCA GTGCTGTCCT 
GAACAACATT TTGTGCACAT ATTGGAGCCC TTCTTGAAGA TTATCACGAA ATCATCCCGG 
ATAAATACAA CACTAGCTAT TAATGGGTTG ACACCCTTGC TTATTGCAAG GCTTGACCAT 
CAGGATGCTA TAGCTCGTCT TAATCTGCTC AAACTAATAA AGTCTGTTTA TGAGCACCAT 
CCACGGCCAA AGCAACTGAT CGTGGAGAAT GATCTGCCTC AGAAGCTTCA GAATCTGATT 
GAGGAGCGCA GAGATGGGCA ACGTTCTGGA GGCCAAGTGT TGGTTAAACA GATGGCTACT 
TCTCTCCTTA AAGCACTCCA TATCAACACC GTTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100254261 ( XM_002277286.4 ) cDNA ORF clone, Vitis vinifera(wine grape) -> XP_002277322.2 Vitis vinifera MAP3K epsilon protein kinase 1 (LOC100254261), transcript variant X1, mRNA.

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CloneID	OVa00542
Clone ID Related Accession (Same CDS sequence)	XM_002277286.4
Accession Version	XM_002277286.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-21
Organism	Vitis vinifera(wine grape)
Product	MAP3K epsilon protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_012007.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 22, 2016 this sequence version replaced XM_002277286.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Vitis vinifera Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC AAGTGGCCAC GTCGCATTTC CACAAATCGA AGACTCTCGA CAACAAATAT 
ATGCTTGGAG ATGAGATCGG AAAAGGAGCA TATGGTCGAG TTTACAAGGG TTTGGATTTG 
GAGAATGGAG ACTTTGTTGC AATTAAACAA GTTTCTTTGG AGAATATTGC TCAAGAAGAT 
CTCAACATTA TCATGCAAGA GATTGATCTG CTCAAGAATT TGAACCACAA AAACATTGTG 
AAATATCTTG GATCTTTGAA GACAAGGAGT CATCTGCACA TAATTCTTGA GTATGTGGAG 
AATGGCTCAC TTGCAAACAT CATCAAACCA AATAAATTTG GGCCATTTCC AGAATCACTG 
GTGGCTGTTT ATATCTCTCA GGTCTTGGAG GGCTTGGTTT ATCTACATGA ACAAGGTGTC 
ATCCATCGGG ATATCAAGGG CGCAAATATA TTGACAACCA AAGAGGGTCT TGTGAAACTT 
GCTGACTTTG GTGTTGCAAC AAAATTAACT GAGGCAGATG TTAATACGCA TTCCGTTGTT 
GGGACACCAT ATTGGATGGC CCCTGAGGTA ATCGAAATGT CTGGGGTTTG TGCTGCTTCG 
GACATTTGGA GTGTTGGTTG CACTGTGATT GAGCTTCTTA CATGTGTACC TCCTTACTAT 
GATCTCCAGC CTATGCCAGC TCTATTCCGT ATTGTTCAGG ATGATCATCC TCCAATACCA 
GATGGCTTAT CTACTGACAT TACAGATTTT CTGCGGCAGT GTTTTAAGAA GGATGCTAGG 
CAGAGGCCTG ATGCAAAGAC ACTGCTTTCA CATCCTTGGA TACGGAACTG TAGGCGTGCT 
TTGCAGTCCT CTCTTCGTCA TAGTGGAACA TTGAGAAATA TACAGGAAGA TGCTTCAGTT 
GATGCAGAGA TTTCCAATGG AGATGATCAA AGTATTGGTG AAAGCCCTTC TGATGAGAAA 
GCAGAAGTAA TTGCCTCTGA ATTTGAAAAT GATTCCAGAA AAGAGTGTTT ACCAACTGAG 
GTTGTTGATA CCGGCAAATC TTATACGGAT TCCAATGGAG ATCTTATTGA GGATGAAGTT 
GATAATCCAG AAGAGTATGT TCAATCAGAT CAAGTACCCA CATTGGCCAT TCACGAAAAA 
TCATCTCTAC CAACTACTTC TGGTATATTG CCTGCTAATA AGGATGTGGC TCCCCCTAGT 
CCAACAGATT CTAACGAGGT ACTAGATATG GGTGATCAGG ATGAAGCACT AATGAATGGT 
AAGGTGGGAT CTCCTAAGTC AAGAAAGAAC AATGTTTTAT ATAAAAAGAG TGAAGGGAAA 
GGAAGTTCTA CTCAACTTGA CAACAGATTA TTTGGTTTTG TACCAAGAAG TCAGGAGAAT 
AGTTCTCGAA AGGCTGCAAA GGCACCAGTC ATTTCGGGCG GAAATGAGCT AAGTAAATTT 
AGTGACACGC CAGGAGATGC TTCTTTGGAA GATTTATTTC ATCCACTGCA TAAGAATCCT 
GAGGATCAGG CAGCTGAAGC TTCAACATCT GCATCCTCAT CACATGTTGT ACAAGGCAAT 
GCATTCATAA ATGATGCTGG AAAAAATGAC CTGGCAACAA AGTTGAGGGC TACAATTGCT 
CAGAAACAAA TGGAGAATGA GATTGGGCAA ACAAATGGAG ATCTCTTCAG TTTAATGTTG 
GATGTTCTGA AGGAGGACGT GATGGATATT GATGGTTTGG TTTTTGATGA CAAAATGCCT 
GGAGAGAACC TTTTTCCCCT ACAGGCTGTT GAGTTTAGCA GATTAGTTGG GTCATTAAGA 
CCACAGGAAC CAGAAGATGT GATCGTGTCT GCCTGTTTGA AGCTTATATC CATTTTTCAC 
CAACGCCCTG AGCAGAAAGG TGTGTTTGTT ACTCAGCATG GTTTACTCCC CCTCATGGAA 
CTACTTGAAG TTTCTAGAAC TCGTGTTATA TGTTCAGTGC TTCAAATTGT AAATCAGATA 
ATCAAGGATA ATACCGATTT CCAGGAAAAT GCTTGTCTTG TTGGCCTGAT CCCTGTTGTT 
ATGAGCTTTG CAGTACCTGA TCATCCCCGG GAAGTTCGCA TGGAAGCCGC TTATTTCTCG 
CAGCAGCTTT GTCAGTCTAG CTCCTTGACA TTGCAAATGT TCATTGCTTG TGGTGGAATA 
CCTGTTTTAG TGGGCTTTCT GGAGGCTGAT TATGTGAGGT ACAGGGAAAT GGTTCATCTA 
GCTATTGATG GCATGTGGCA GGTCTTCAAG CTTCAGCGAT CAACACCTAG AAATGATTTC 
TGTCGCATTG CTGCAAAGAA TGGAATACTT CCTAGGCTTA TAAACACTCT TTATAGTTTG 
AATGAGGCTG CTCGTCTAGC TTCCATAGCT GGTGGTAGTG GATTTACAAT AGAAGGTTTG 
GCTCCGCGAC CACGTTCTGG TTCACTGGAT CCCAGCTCTC CTATTTTTAT TCAGGGTGAG 
ATATCACTTA CTGGAATCGA TCATCCTGAT CTCCTTAAGG TTAGGCATGG GCTAATTGAT 
CATTCCTTGT CAACTGCAAC CCAAGAACCT TCACGTGTTT CAGCATCACA TCCCCAAAGG 
TCAGATGCTA ACCAACCAGA TTCCCGATAC TTCTCTCTGG ATACTGACAG GCCTGCAATG 
GAAGCTTCAG TTGCTTCTAA ATTACAAGAC CTTGCATTTT CAGAAAAAGT TGCAAATATG 
CAAACAAAGG AATCTTCTGG AACTATTTTG AAAGAGCGAG AAAACTTGGA CCGCTGGAAA 
ATTGATCCAC AACGAGTCCC TAATTCGGCT AATAGGACAT CAGTAGACAG ACCTTCAAAA 
TTGGTGGAAG GTGTGTCAAA TGGGTTTCCT AGTACAATAG GGACCCAGCA AGAGCAAGTT 
CGACCTCTTC TTAGCTTGTT GGATAAGGAG CCCCCTTCCC GGCATTTTTC TGGTCAGCTA 
GAGTATGTAC GGCACCTCTC TGGGTTGGAG AGACATGAAA GCATACTGCC TCTACTACAT 
GCAACCAATG AGAAGAAAAC AAATGGTGAA CTAGATTTTC TGATGGCAGA ATTTGCAGAG 
GTTTCTGGTC GTGGAAGAGA AAATGGAAAT CTGGATTCTG CACCTAGAAT TTCAAATAAA 
ACTGTAAATA AGAAGATTCC ATTGGCATCT AATGAGGGTG CTGCTTCTAC ATCTGGGATT 
GCTTCTCAGA CAGCATCAGG TGTACTGTCT GGTTCTGGTG TTTTAAATGC TAGGCCAGGA 
AGTGCAACGT CATCTGGGTT GCTTTCCCAT ATGGTATCAT CACTCAATGC TGATGTTGCT 
AAGGAATATC TAGAAAAGGT TGCAGACCTT CTGCTGGAGT TTGCCCAAGC AGATACGACA 
GTAAAATCCT ACATGTGTAG CCAAAGCTTG CTTAGCCGTC TTTTTCAGAT GTTCAATCGG 
ATAGAGCCGC CTATTCTTTT GAAGATACTA AAGTGTATCA ATCATTTGTC AACCGACCCA 
AATTGCTTAG AAAATCTTCA GCGTGCCGAT GCAATCAAAT ACTTGATCCC AAACCTTGAA 
CTCAAAGAAG GGCCTCTTGT ATTTCAAATA CATTATGAGG TCCTCTATGC ATTATTCAAC 
CTCTGCAAGA TAAATAAGAG GAGGCAAGAA CAAGCAGCTG AAAATGGAAT CATTCCACAC 
TTGATGCATT TTATAATGTC AGATTCTCCC TTGAAACAAC ATGCATTGCC TCTACTGTGC 
GATATGGCCC ATGCATCACG TAACTCAAGG GAGCAATTAA GGGCCCATGA TGGGTTGGAT 
GTGTACTTAA GCCTACTTGA GGATGAGCTT TGGTCTGTGA CAGCATTAGA TTCTATTGCT 
GTGTGCTTGG CCCATGACAA TGACAACCGG AAAGTAGAAC AGGCGTTGCT GAAAAAGGAT 
GCAATTCAGA AATTGGTCAA ATTCTTCCAG TGCTGTCCTG AACAACATTT TGTGCACATA 
TTGGAGCCCT TCTTGAAGAT TATCACGAAA TCATCCCGGA TAAATACAAC ACTAGCTATT 
AATGGGTTGA CACCCTTGCT TATTGCAAGG CTTGACCATC AGGATGCTAT AGCTCGTCTT 
AATCTGCTCA AACTAATAAA GTCTGTTTAT GAGCACCATC CACGGCCAAA GCAACTGATC 
GTGGAGAATG ATCTGCCTCA GAAGCTTCAG AATCTGATTG AGGAGCGCAG AGATGGGCAA 
CGTTCTGGAG GCCAAGTGTT GGTTAAACAG ATGGCTACTT CTCTCCTTAA AGCACTCCAT 
ATCAACACCG TTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002277322.2
CDS	270..4547
Translation	MSRQVATSHFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNIIMQEIDLLKNLNHKNIVKYLGSLKTRSHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQDDHPPIPDGLSTDITDFLRQCFKKDARQRPDAKTLLSHPWIRNCRRALQSSLRHSGTLRNIQEDASVDAEISNGDDQSIGESPSDEKAEVIASEFENDSRKECLPTEVVDTGKSYTDSNGDLIEDEVDNPEEYVQSDQVPTLAIHEKSSLPTTSGILPANKDVAPPSPTDSNEVLDMGDQDEALMNGKVGSPKSRKNNVLYKKSEGKGSSTQLDNRLFGFVPRSQENSSRKAAKAPVISGGNELSKFSDTPGDASLEDLFHPLHKNPEDQAAEASTSASSSHVVQGNAFINDAGKNDLATKLRATIAQKQMENEIGQTNGDLFSLMLDVLKEDVMDIDGLVFDDKMPGENLFPLQAVEFSRLVGSLRPQEPEDVIVSACLKLISIFHQRPEQKGVFVTQHGLLPLMELLEVSRTRVICSVLQIVNQIIKDNTDFQENACLVGLIPVVMSFAVPDHPREVRMEAAYFSQQLCQSSSLTLQMFIACGGIPVLVGFLEADYVRYREMVHLAIDGMWQVFKLQRSTPRNDFCRIAAKNGILPRLINTLYSLNEAARLASIAGGSGFTIEGLAPRPRSGSLDPSSPIFIQGEISLTGIDHPDLLKVRHGLIDHSLSTATQEPSRVSASHPQRSDANQPDSRYFSLDTDRPAMEASVASKLQDLAFSEKVANMQTKESSGTILKERENLDRWKIDPQRVPNSANRTSVDRPSKLVEGVSNGFPSTIGTQQEQVRPLLSLLDKEPPSRHFSGQLEYVRHLSGLERHESILPLLHATNEKKTNGELDFLMAEFAEVSGRGRENGNLDSAPRISNKTVNKKIPLASNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSSLNADVAKEYLEKVADLLLEFAQADTTVKSYMCSQSLLSRLFQMFNRIEPPILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGPLVFQIHYEVLYALFNLCKINKRRQEQAAENGIIPHLMHFIMSDSPLKQHALPLLCDMAHASRNSREQLRAHDGLDVYLSLLEDELWSVTALDSIAVCLAHDNDNRKVEQALLKKDAIQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTLAINGLTPLLIARLDHQDAIARLNLLKLIKSVYEHHPRPKQLIVENDLPQKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL

Target ORF information:

RefSeq Version	XM_002277286.4
Organism	Vitis vinifera(wine grape)
Definition	Vitis vinifera MAP3K epsilon protein kinase 1 (LOC100254261), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002277286.4

ORF Insert Sequence:

ATGTCTCGCC AAGTGGCCAC GTCGCATTTC CACAAATCGA AGACTCTCGA CAACAAATAT 
ATGCTTGGAG ATGAGATCGG AAAAGGAGCA TATGGTCGAG TTTACAAGGG TTTGGATTTG 
GAGAATGGAG ACTTTGTTGC AATTAAACAA GTTTCTTTGG AGAATATTGC TCAAGAAGAT 
CTCAACATTA TCATGCAAGA GATTGATCTG CTCAAGAATT TGAACCACAA AAACATTGTG 
AAATATCTTG GATCTTTGAA GACAAGGAGT CATCTGCACA TAATTCTTGA GTATGTGGAG 
AATGGCTCAC TTGCAAACAT CATCAAACCA AATAAATTTG GGCCATTTCC AGAATCACTG 
GTGGCTGTTT ATATCTCTCA GGTCTTGGAG GGCTTGGTTT ATCTACATGA ACAAGGTGTC 
ATCCATCGGG ATATCAAGGG CGCAAATATA TTGACAACCA AAGAGGGTCT TGTGAAACTT 
GCTGACTTTG GTGTTGCAAC AAAATTAACT GAGGCAGATG TTAATACGCA TTCCGTTGTT 
GGGACACCAT ATTGGATGGC CCCTGAGGTA ATCGAAATGT CTGGGGTTTG TGCTGCTTCG 
GACATTTGGA GTGTTGGTTG CACTGTGATT GAGCTTCTTA CATGTGTACC TCCTTACTAT 
GATCTCCAGC CTATGCCAGC TCTATTCCGT ATTGTTCAGG ATGATCATCC TCCAATACCA 
GATGGCTTAT CTACTGACAT TACAGATTTT CTGCGGCAGT GTTTTAAGAA GGATGCTAGG 
CAGAGGCCTG ATGCAAAGAC ACTGCTTTCA CATCCTTGGA TACGGAACTG TAGGCGTGCT 
TTGCAGTCCT CTCTTCGTCA TAGTGGAACA TTGAGAAATA TACAGGAAGA TGCTTCAGTT 
GATGCAGAGA TTTCCAATGG AGATGATCAA AGTATTGGTG AAAGCCCTTC TGATGAGAAA 
GCAGAAGTAA TTGCCTCTGA ATTTGAAAAT GATTCCAGAA AAGAGTGTTT ACCAACTGAG 
GTTGTTGATA CCGGCAAATC TTATACGGAT TCCAATGGAG ATCTTATTGA GGATGAAGTT 
GATAATCCAG AAGAGTATGT TCAATCAGAT CAAGTACCCA CATTGGCCAT TCACGAAAAA 
TCATCTCTAC CAACTACTTC TGGTATATTG CCTGCTAATA AGGATGTGGC TCCCCCTAGT 
CCAACAGATT CTAACGAGGT ACTAGATATG GGTGATCAGG ATGAAGCACT AATGAATGGT 
AAGGTGGGAT CTCCTAAGTC AAGAAAGAAC AATGTTTTAT ATAAAAAGAG TGAAGGGAAA 
GGAAGTTCTA CTCAACTTGA CAACAGATTA TTTGGTTTTG TACCAAGAAG TCAGGAGAAT 
AGTTCTCGAA AGGCTGCAAA GGCACCAGTC ATTTCGGGCG GAAATGAGCT AAGTAAATTT 
AGTGACACGC CAGGAGATGC TTCTTTGGAA GATTTATTTC ATCCACTGCA TAAGAATCCT 
GAGGATCAGG CAGCTGAAGC TTCAACATCT GCATCCTCAT CACATGTTGT ACAAGGCAAT 
GCATTCATAA ATGATGCTGG AAAAAATGAC CTGGCAACAA AGTTGAGGGC TACAATTGCT 
CAGAAACAAA TGGAGAATGA GATTGGGCAA ACAAATGGAG ATCTCTTCAG TTTAATGTTG 
GATGTTCTGA AGGAGGACGT GATGGATATT GATGGTTTGG TTTTTGATGA CAAAATGCCT 
GGAGAGAACC TTTTTCCCCT ACAGGCTGTT GAGTTTAGCA GATTAGTTGG GTCATTAAGA 
CCACAGGAAC CAGAAGATGT GATCGTGTCT GCCTGTTTGA AGCTTATATC CATTTTTCAC 
CAACGCCCTG AGCAGAAAGG TGTGTTTGTT ACTCAGCATG GTTTACTCCC CCTCATGGAA 
CTACTTGAAG TTTCTAGAAC TCGTGTTATA TGTTCAGTGC TTCAAATTGT AAATCAGATA 
ATCAAGGATA ATACCGATTT CCAGGAAAAT GCTTGTCTTG TTGGCCTGAT CCCTGTTGTT 
ATGAGCTTTG CAGTACCTGA TCATCCCCGG GAAGTTCGCA TGGAAGCCGC TTATTTCTCG 
CAGCAGCTTT GTCAGTCTAG CTCCTTGACA TTGCAAATGT TCATTGCTTG TGGTGGAATA 
CCTGTTTTAG TGGGCTTTCT GGAGGCTGAT TATGTGAGGT ACAGGGAAAT GGTTCATCTA 
GCTATTGATG GCATGTGGCA GGTCTTCAAG CTTCAGCGAT CAACACCTAG AAATGATTTC 
TGTCGCATTG CTGCAAAGAA TGGAATACTT CCTAGGCTTA TAAACACTCT TTATAGTTTG 
AATGAGGCTG CTCGTCTAGC TTCCATAGCT GGTGGTAGTG GATTTACAAT AGAAGGTTTG 
GCTCCGCGAC CACGTTCTGG TTCACTGGAT CCCAGCTCTC CTATTTTTAT TCAGGGTGAG 
ATATCACTTA CTGGAATCGA TCATCCTGAT CTCCTTAAGG TTAGGCATGG GCTAATTGAT 
CATTCCTTGT CAACTGCAAC CCAAGAACCT TCACGTGTTT CAGCATCACA TCCCCAAAGG 
TCAGATGCTA ACCAACCAGA TTCCCGATAC TTCTCTCTGG ATACTGACAG GCCTGCAATG 
GAAGCTTCAG TTGCTTCTAA ATTACAAGAC CTTGCATTTT CAGAAAAAGT TGCAAATATG 
CAAACAAAGG AATCTTCTGG AACTATTTTG AAAGAGCGAG AAAACTTGGA CCGCTGGAAA 
ATTGATCCAC AACGAGTCCC TAATTCGGCT AATAGGACAT CAGTAGACAG ACCTTCAAAA 
TTGGTGGAAG GTGTGTCAAA TGGGTTTCCT AGTACAATAG GGACCCAGCA AGAGCAAGTT 
CGACCTCTTC TTAGCTTGTT GGATAAGGAG CCCCCTTCCC GGCATTTTTC TGGTCAGCTA 
GAGTATGTAC GGCACCTCTC TGGGTTGGAG AGACATGAAA GCATACTGCC TCTACTACAT 
GCAACCAATG AGAAGAAAAC AAATGGTGAA CTAGATTTTC TGATGGCAGA ATTTGCAGAG 
GTTTCTGGTC GTGGAAGAGA AAATGGAAAT CTGGATTCTG CACCTAGAAT TTCAAATAAA 
ACTGTAAATA AGAAGATTCC ATTGGCATCT AATGAGGGTG CTGCTTCTAC ATCTGGGATT 
GCTTCTCAGA CAGCATCAGG TGTACTGTCT GGTTCTGGTG TTTTAAATGC TAGGCCAGGA 
AGTGCAACGT CATCTGGGTT GCTTTCCCAT ATGGTATCAT CACTCAATGC TGATGTTGCT 
AAGGAATATC TAGAAAAGGT TGCAGACCTT CTGCTGGAGT TTGCCCAAGC AGATACGACA 
GTAAAATCCT ACATGTGTAG CCAAAGCTTG CTTAGCCGTC TTTTTCAGAT GTTCAATCGG 
ATAGAGCCGC CTATTCTTTT GAAGATACTA AAGTGTATCA ATCATTTGTC AACCGACCCA 
AATTGCTTAG AAAATCTTCA GCGTGCCGAT GCAATCAAAT ACTTGATCCC AAACCTTGAA 
CTCAAAGAAG GGCCTCTTGT ATTTCAAATA CATTATGAGG TCCTCTATGC ATTATTCAAC 
CTCTGCAAGA TAAATAAGAG GAGGCAAGAA CAAGCAGCTG AAAATGGAAT CATTCCACAC 
TTGATGCATT TTATAATGTC AGATTCTCCC TTGAAACAAC ATGCATTGCC TCTACTGTGC 
GATATGGCCC ATGCATCACG TAACTCAAGG GAGCAATTAA GGGCCCATGA TGGGTTGGAT 
GTGTACTTAA GCCTACTTGA GGATGAGCTT TGGTCTGTGA CAGCATTAGA TTCTATTGCT 
GTGTGCTTGG CCCATGACAA TGACAACCGG AAAGTAGAAC AGGCGTTGCT GAAAAAGGAT 
GCAATTCAGA AATTGGTCAA ATTCTTCCAG TGCTGTCCTG AACAACATTT TGTGCACATA 
TTGGAGCCCT TCTTGAAGAT TATCACGAAA TCATCCCGGA TAAATACAAC ACTAGCTATT 
AATGGGTTGA CACCCTTGCT TATTGCAAGG CTTGACCATC AGGATGCTAT AGCTCGTCTT 
AATCTGCTCA AACTAATAAA GTCTGTTTAT GAGCACCATC CACGGCCAAA GCAACTGATC 
GTGGAGAATG ATCTGCCTCA GAAGCTTCAG AATCTGATTG AGGAGCGCAG AGATGGGCAA 
CGTTCTGGAG GCCAAGTGTT GGTTAAACAG ATGGCTACTT CTCTCCTTAA AGCACTCCAT 
ATCAACACCG TTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100254261 cDNA ORF clone, Vitis vinifera(wine grape)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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