cit cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cit
Entrez Gene ID	100145758
Full Name	citron rho-interacting serine/threonine kinase
Synonyms	crik,stk21,xcit
General protein information	Preferred Names citron rho-interacting serine/threonine kinase Names citron Rho-interacting kinase citron (rho-interacting, serine/threonine kinase 21)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031895834.1	XP_031751694.1	citron Rho-interacting kinase isoform X2
XM_012956618.2	XP_012812072.1	citron Rho-interacting kinase isoform X2
XM_031895833.1	XP_031751693.1	citron Rho-interacting kinase isoform X1
XM_031895838.1	XP_031751698.1	citron Rho-interacting kinase isoform X3
XM_031895842.1	XP_031751702.1	citron Rho-interacting kinase isoform X4
XM_031895845.1	XP_031751705.1	citron Rho-interacting kinase isoform X4
XM_012956619.2	XP_012812073.1	citron Rho-interacting kinase isoform X1
NM_001127129.1	NP_001120601.1	citron Rho-interacting kinase

Pathways from BioSystems

Homologies

Homo sapiens (human)	CIT	NP_001193928.1
Pan troglodytes (chimpanzee)	CIT	XP_003952361.1
Bos taurus (cattle)	CIT	XP_002694628.3
Rattus norvegicus (Norway rat)	Cit	NP_001025082.1
Drosophila melanogaster (fruit fly)	sti	NP_001261768.1
Canis lupus familiaris (dog)	CIT	XP_003433432.1
Mus musculus (house mouse)	Cit	NP_031734.3
Danio rerio (zebrafish)	LOC100329792	XP_005171785.1
	CIT	XP_001085560.1
Gallus gallus (chicken)	CIT	XP_004934462.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006932	XP_308829.4
Xenopus tropicalis (tropical clawed frog)	cit	NP_001120601.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cit gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cit cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa47600	XM_031895833.1 Latest version!	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa47601	XM_031895834.1 Latest version!	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa47602	XM_031895838.1 Latest version!	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa47603	XM_031895842.1 Latest version!	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa47603	XM_031895845.1 Latest version!	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa02127	NM_001127129.1 Latest version!	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa32034	XM_012956618.2 Latest version!	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OXa32035	XM_012956619.2 Latest version!	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cit ( XM_031895833.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751693.1 Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa47600
Clone ID Related Accession (Same CDS sequence)	XM_031895833.1
Accession Version	XM_031895833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCCTGC CCGTATTTTG AATCAGCTAA ATGACTTGGA AAAGAAGCAT 
GCAATGCTGG AAATGAATGC TCGCAGTTTG CAACAAAAAC TGGAGACTGA AAGAGAACTA 
AAGCAACGGC TTTTAGAAGA GCAGGCTAAG TTGCAACAGC AGATGGACCT GCAAAAGAAT 
CACATTTTCC GCCTGACCCA AGGACTTCAG GAGGCCCTGG ATCGAGCTGA TCTGCTTAAA 
ACAGAAAGAA GTGATTTGGA GTATCAACTG GAAAACATGC AGGCACTCTT TTCTCATGAA 
AAAGTAAAGA TGGAAGGAAC CATTTCCCAA CAAACAAAAC TCATTGACTT CTTACAAGCA 
AAAATGGATC AGCCATCTAA GAAGAAAAAG GGTTTGTTCA GTAGGCGCAG AGATGAACCC 
AATCAAACCT CACAGGTTCC ATTACAGTAC AATGAGCTAA AAGTAGCGCT TGAAAAAGAG 
AAGGCTAGAA CTGCAGAATT AGAAGAGGCT CTTCAAAAAA CACGAATTGA ACTCCGCTCT 
GCAAGAGAGG AAGCTGCTCA CTTAAAGGTG TCAGATCATC CTGTTCCTTC TACACCTGGT 
ACAGCAAGAC AACAGATTAT CATGTCTGCA CTGGTCAGAT CACCTGAGCA TCAGCCCAAT 
GCAGTCAGCC TGCTTGCCCC ACAGAACAGC AGAAGGAAAG AATCATCAGC TCCTGAAGAA 
TTCAGTCGCC GACTTAAAGA AAGAATGCAT CATAATATTC CACACAGATT TAATGTTGCA 
CTTACCATGA GAGCAGCAAA ATGTTCGGTG TGCTTGGATA CTGTTCACTT TGGGCGGCAG 
GGAGCAAAGT GCCTAGAATG TCAGGTCATG TGTCACCCAA AGTGTTCCTC CTGCCTCCCA 
GCTACATGTG GCCTCCCTGC AGAATATGCT TCACATTTCA CTGAAGCCTT TTGTAGAGAG 
AAAATGAATT CGCCTGGAAT GCAGACGAAG GAAGCAAATG GTGTTGTGCG ACTTGAAGGC 
TGGATGAAAA TTCCGAGGAA TAACAAACGT GGCCAGCAAG GATGGGACAG GAAATATGTG 
GTCTTGGAAG GGTCTAAAGT GCTGATCTAT GATACTGAAT TAAAAGAAGG AGGACAACGG 
CCTTCAGAGG AGTTTGAGCT TTGTCTTCCT GATGGAGATG TAACCGTTCA TGGTGCCGTT 
GGATCTACAG AGCTTGTAAA TACTGCAAAA TCAGATGTGC CATACGTCAT GAAGATTGAG 
TCTCATCCAC ACACAACTTG TTGGCCAGGG CGCACTATGT ACTTGTTAGC GCCAAGTTTT 
CCTGATAAGC AGCGTTGGGT TAATGCACTG GAGTCTGTGG TTGCAGGAGG AAGGGTGTCC 
AGAGAAAAAG CAGAGGCTGA TGCTAAACTG CTTGGAAACT CTTTGTTAAA ATTGGAGGGG 
GAAGACCGTC TTGATATAAA TTGCACAATA CCTCTAACTG AGCAGATTGT TTTGGTTGGC 
GCAGAGGAGG GTCTTTATGC TCTGAATGTG CTGAAGAATT CTCTCACTCA CATACCAGGT 
ATAGGAGCAG TATTCCAGAT TCATATTGTT AAGGAGCTGG ATAAACTTCT TTTAATAGCA 
GGGGAAGAAA GGGCTCTATG CCTTGTTGAT GTGAAGAAGG TGAAGCAGTC ATTGGCTCAG 
TCACATCTAC CAGCACAGCC AGAAATCTCA ATGACTGTTT TTGAAGCTGT TAAAGGATGT 
CACCTTTTTG CTGCAGGAAA GATTGAAAAT GGGTTATGCA TCTGTGCAGC CATGTTAAAT 
AAAGTTGTCA TACTGCGCTA CAATGAAAGT CTCGGCAAGT TTTGCATAAG AAAGGAAATT 
GAAACTTCTG AGCCATGCAG TTGCATTTAT TTCACTGGCT ACAGTGTCAT CATTGGAACA 
AACAAGTTCT ATGAGATTGA GATGAAGCAG TACACTCTGG AGGAGTTCTT GGATAAAAAT 
GACCATTCCT TGGCTTCTGC TGTGTTTGCT TCATCTTCCA ATAGCTTCCC AATAGCTATA 
ATGCAAGTAA ACAGCACAGG CCAGAGAGAA GAATACCTCC TTTGCTTCCA TGAGTTCGGT 
GTATTTGTTG ATTCTTATGG AAGACGTAGT CGTACTGAAG ATTTGAAATG GAGTCGGCTT 
CCTCTTGCAT TTGCCTACAG GGAACCGTAC TTATTTGTGA CCCATTTTAA CTCTTTGGAG 
GTGATTGAGA TCCAGGGACG TGCTGCACTA GGTGCTCCAG CTCGAGCCCA TCTTGATATT 
CCCAACCCAC GTTACCTGGG ACCAGCAATC TCTTCTGGAG CCATTTATAT TGCATCTGTT 
TACCAGGATA AGCTGAGAGT AATCTGTTGC AAGGGAAATC TAGTAAAGGA CACTACAAGT 
GAACTTCATA GGGCTCCTTC TACAACTCGC AGCCCAAACA AGAGAGGACC ACCATCTTAC 
AATGACCATA TAACAAAGCG AATAGCATCC AGCCCAGTGC CAACTGAGGT CCAAGGTCTC 
GTACGGGAGC CTAGTACACC TCATAGATAC CGCGAAGGAC GGACAGAAAT GCGCAGAGAC 
AAATCTCCTG GGAGGCCTTT GGACAGGGAG AAGTCTCCAG GGAGGTTACT GAGCACCAGA 
CGGGAGAGGT CACCTGGAAG GCTTTTTGAA GAGAGCAACA GGAGCCGTGG AGCTGTAAGG 
ACACCCTTGT CACAAGTCAA TAAGGTTTGG GACCAGTCTT CTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751693.1
CDS	3..6230
Translation	MLKFKFGSRSAADAISSDPITSRAARLNNFFQGKSSCQFPFQQQLNVLSREGLLDSLCALYEECCNPALMKIKHVSNFVKKYSDTVAELRELQPNVKDFEIKNVVGRGHFAEVHVVKEKVTGDIFAMKVMKKKALLAQDQVSFFEEERNILSHHNSPWIPQLHYAFQDKENLYLVMQYQPGGDLFALMNRYDEQFDENMAQFYLAELVLAIYSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKLTANKTVNAKLPVGTPDYIAPEVLMVMNGDGRSTYGPECDWWSLGIVAYEMMYGKSPFTEGTSIKTFNNIMNFQRFLKFPEEPKLSSNFLDLIQSLICGQKERLKYEDLCCHPFFSNTDWDRLRNTPPPFVPTLKSDDDTSNFDEPEKNLRILPSSCQLNSAGFSGDDLPFVGFSFIKALSLLNKSESVVSGLDSPAKVSSMEKKLFHKSKELQDTQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLNDIREQSRKLQDIKEQEFQAQLEEMKMMMHQLEEDLLAARRRSDLYETELRDSRLAAEDFKRKAVECQQKLQKAKDHGKGEVGELHTKLEKVNADQQNKIQELQEKLAKAVKASSEATELLQNIRQAKERAEKELEKLKNRDDSNDAIKKKLLDAEERRHSLENQVRRLETVERRENKLKEDIQTKSEQIQQMAEKIMELEERYREAQVGAQRLEVHLKQNEQVYEEKARNIETQMKKDLADKEALELSRIKNEEEICEKNRILSEQKAMINALESKITSLEQRLAELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKIEAQNQKLEEQLERINNQDHTDKNRLLELETRLREISLEHEEQKLELKRQLTEVQLTLQERETQISSLQSARSALESQLHQAKTELEDTTAEAEEEIQVLRSHRDEIQRKFDTLRDSCTVLTELEDQLNRISQENADLNNQNFYLSKQLSEVSGATDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWESWRSSLEDEKTQFECRLREVQRLLDTEKQSRVRSDQRTTESRQMVELAAKEHKAEILALQQALKDQKLKTESLSDKPARILNQLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENMQALFSHEKVKMEGTISQQTKLIDFLQAKMDQPSKKKKGLFSRRRDEPNQTSQVPLQYNELKVALEKEKARTAELEEALQKTRIELRSAREEAAHLKVSDHPVPSTPGTARQQIIMSALVRSPEHQPNAVSLLAPQNSRRKESSAPEEFSRRLKERMHHNIPHRFNVALTMRAAKCSVCLDTVHFGRQGAKCLECQVMCHPKCSSCLPATCGLPAEYASHFTEAFCREKMNSPGMQTKEANGVVRLEGWMKIPRNNKRGQQGWDRKYVVLEGSKVLIYDTELKEGGQRPSEEFELCLPDGDVTVHGAVGSTELVNTAKSDVPYVMKIESHPHTTCWPGRTMYLLAPSFPDKQRWVNALESVVAGGRVSREKAEADAKLLGNSLLKLEGEDRLDINCTIPLTEQIVLVGAEEGLYALNVLKNSLTHIPGIGAVFQIHIVKELDKLLLIAGEERALCLVDVKKVKQSLAQSHLPAQPEISMTVFEAVKGCHLFAAGKIENGLCICAAMLNKVVILRYNESLGKFCIRKEIETSEPCSCIYFTGYSVIIGTNKFYEIEMKQYTLEEFLDKNDHSLASAVFASSSNSFPIAIMQVNSTGQREEYLLCFHEFGVFVDSYGRRSRTEDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQGRAALGAPARAHLDIPNPRYLGPAISSGAIYIASVYQDKLRVICCKGNLVKDTTSELHRAPSTTRSPNKRGPPSYNDHITKRIASSPVPTEVQGLVREPSTPHRYREGRTEMRRDKSPGRPLDREKSPGRLLSTRRERSPGRLFEESNRSRGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_031895833.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895833.1

ORF Insert Sequence:

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCCTGC CCGTATTTTG AATCAGCTAA ATGACTTGGA AAAGAAGCAT 
GCAATGCTGG AAATGAATGC TCGCAGTTTG CAACAAAAAC TGGAGACTGA AAGAGAACTA 
AAGCAACGGC TTTTAGAAGA GCAGGCTAAG TTGCAACAGC AGATGGACCT GCAAAAGAAT 
CACATTTTCC GCCTGACCCA AGGACTTCAG GAGGCCCTGG ATCGAGCTGA TCTGCTTAAA 
ACAGAAAGAA GTGATTTGGA GTATCAACTG GAAAACATGC AGGCACTCTT TTCTCATGAA 
AAAGTAAAGA TGGAAGGAAC CATTTCCCAA CAAACAAAAC TCATTGACTT CTTACAAGCA 
AAAATGGATC AGCCATCTAA GAAGAAAAAG GGTTTGTTCA GTAGGCGCAG AGATGAACCC 
AATCAAACCT CACAGGTTCC ATTACAGTAC AATGAGCTAA AAGTAGCGCT TGAAAAAGAG 
AAGGCTAGAA CTGCAGAATT AGAAGAGGCT CTTCAAAAAA CACGAATTGA ACTCCGCTCT 
GCAAGAGAGG AAGCTGCTCA CTTAAAGGTG TCAGATCATC CTGTTCCTTC TACACCTGGT 
ACAGCAAGAC AACAGATTAT CATGTCTGCA CTGGTCAGAT CACCTGAGCA TCAGCCCAAT 
GCAGTCAGCC TGCTTGCCCC ACAGAACAGC AGAAGGAAAG AATCATCAGC TCCTGAAGAA 
TTCAGTCGCC GACTTAAAGA AAGAATGCAT CATAATATTC CACACAGATT TAATGTTGCA 
CTTACCATGA GAGCAGCAAA ATGTTCGGTG TGCTTGGATA CTGTTCACTT TGGGCGGCAG 
GGAGCAAAGT GCCTAGAATG TCAGGTCATG TGTCACCCAA AGTGTTCCTC CTGCCTCCCA 
GCTACATGTG GCCTCCCTGC AGAATATGCT TCACATTTCA CTGAAGCCTT TTGTAGAGAG 
AAAATGAATT CGCCTGGAAT GCAGACGAAG GAAGCAAATG GTGTTGTGCG ACTTGAAGGC 
TGGATGAAAA TTCCGAGGAA TAACAAACGT GGCCAGCAAG GATGGGACAG GAAATATGTG 
GTCTTGGAAG GGTCTAAAGT GCTGATCTAT GATACTGAAT TAAAAGAAGG AGGACAACGG 
CCTTCAGAGG AGTTTGAGCT TTGTCTTCCT GATGGAGATG TAACCGTTCA TGGTGCCGTT 
GGATCTACAG AGCTTGTAAA TACTGCAAAA TCAGATGTGC CATACGTCAT GAAGATTGAG 
TCTCATCCAC ACACAACTTG TTGGCCAGGG CGCACTATGT ACTTGTTAGC GCCAAGTTTT 
CCTGATAAGC AGCGTTGGGT TAATGCACTG GAGTCTGTGG TTGCAGGAGG AAGGGTGTCC 
AGAGAAAAAG CAGAGGCTGA TGCTAAACTG CTTGGAAACT CTTTGTTAAA ATTGGAGGGG 
GAAGACCGTC TTGATATAAA TTGCACAATA CCTCTAACTG AGCAGATTGT TTTGGTTGGC 
GCAGAGGAGG GTCTTTATGC TCTGAATGTG CTGAAGAATT CTCTCACTCA CATACCAGGT 
ATAGGAGCAG TATTCCAGAT TCATATTGTT AAGGAGCTGG ATAAACTTCT TTTAATAGCA 
GGGGAAGAAA GGGCTCTATG CCTTGTTGAT GTGAAGAAGG TGAAGCAGTC ATTGGCTCAG 
TCACATCTAC CAGCACAGCC AGAAATCTCA ATGACTGTTT TTGAAGCTGT TAAAGGATGT 
CACCTTTTTG CTGCAGGAAA GATTGAAAAT GGGTTATGCA TCTGTGCAGC CATGTTAAAT 
AAAGTTGTCA TACTGCGCTA CAATGAAAGT CTCGGCAAGT TTTGCATAAG AAAGGAAATT 
GAAACTTCTG AGCCATGCAG TTGCATTTAT TTCACTGGCT ACAGTGTCAT CATTGGAACA 
AACAAGTTCT ATGAGATTGA GATGAAGCAG TACACTCTGG AGGAGTTCTT GGATAAAAAT 
GACCATTCCT TGGCTTCTGC TGTGTTTGCT TCATCTTCCA ATAGCTTCCC AATAGCTATA 
ATGCAAGTAA ACAGCACAGG CCAGAGAGAA GAATACCTCC TTTGCTTCCA TGAGTTCGGT 
GTATTTGTTG ATTCTTATGG AAGACGTAGT CGTACTGAAG ATTTGAAATG GAGTCGGCTT 
CCTCTTGCAT TTGCCTACAG GGAACCGTAC TTATTTGTGA CCCATTTTAA CTCTTTGGAG 
GTGATTGAGA TCCAGGGACG TGCTGCACTA GGTGCTCCAG CTCGAGCCCA TCTTGATATT 
CCCAACCCAC GTTACCTGGG ACCAGCAATC TCTTCTGGAG CCATTTATAT TGCATCTGTT 
TACCAGGATA AGCTGAGAGT AATCTGTTGC AAGGGAAATC TAGTAAAGGA CACTACAAGT 
GAACTTCATA GGGCTCCTTC TACAACTCGC AGCCCAAACA AGAGAGGACC ACCATCTTAC 
AATGACCATA TAACAAAGCG AATAGCATCC AGCCCAGTGC CAACTGAGGT CCAAGGTCTC 
GTACGGGAGC CTAGTACACC TCATAGATAC CGCGAAGGAC GGACAGAAAT GCGCAGAGAC 
AAATCTCCTG GGAGGCCTTT GGACAGGGAG AAGTCTCCAG GGAGGTTACT GAGCACCAGA 
CGGGAGAGGT CACCTGGAAG GCTTTTTGAA GAGAGCAACA GGAGCCGTGG AGCTGTAAGG 
ACACCCTTGT CACAAGTCAA TAAGGTTTGG GACCAGTCTT CTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cit ( XM_031895834.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751694.1 Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X2, mRNA.

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CloneID	OXa47601
Clone ID Related Accession (Same CDS sequence)	XM_031895834.1
Accession Version	XM_031895834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	citron Rho-interacting kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCTAAA TGACTTGGAA AAGAAGCATG CAATGCTGGA AATGAATGCT 
CGCAGTTTGC AACAAAAACT GGAGACTGAA AGAGAACTAA AGCAACGGCT TTTAGAAGAG 
CAGGCTAAGT TGCAACAGCA GATGGACCTG CAAAAGAATC ACATTTTCCG CCTGACCCAA 
GGACTTCAGG AGGCCCTGGA TCGAGCTGAT CTGCTTAAAA CAGAAAGAAG TGATTTGGAG 
TATCAACTGG AAAACATGCA GGCACTCTTT TCTCATGAAA AAGTAAAGAT GGAAGGAACC 
ATTTCCCAAC AAACAAAACT CATTGACTTC TTACAAGCAA AAATGGATCA GCCATCTAAG 
AAGAAAAAGG TACAAAATGG TTTGTTCAGT AGGCGCAGAG ATGAACCCAA TCAAACCTCA 
CAGGTTCCAT TACAGTACAA TGAGCTAAAA GTAGCGCTTG AAAAAGAGAA GGCTAGAACT 
GCAGAATTAG AAGAGGCTCT TCAAAAAACA CGAATTGAAC TCCGCTCTGC AAGAGAGGAA 
GCTGCTCACT TAAAGGTGTC AGATCATCCT GTTCCTTCTA CACCTGGTAC AGCAAGACAA 
CAGATTATCA TGTCTGCACT GGTCAGATCA CCTGAGCATC AGCCCAATGC AGTCAGCCTG 
CTTGCCCCAC AGAACAGCAG AAGGAAAGAA TCATCAGCTC CTGAAGAATT CAGTCGCCGA 
CTTAAAGAAA GAATGCATCA TAATATTCCA CACAGATTTA ATGTTGCACT TACCATGAGA 
GCAGCAAAAT GTTCGGTGTG CTTGGATACT GTTCACTTTG GGCGGCAGGG AGCAAAGTGC 
CTAGAATGTC AGGTCATGTG TCACCCAAAG TGTTCCTCCT GCCTCCCAGC TACATGTGGC 
CTCCCTGCAG AATATGCTTC ACATTTCACT GAAGCCTTTT GTAGAGAGAA AATGAATTCG 
CCTGGAATGC AGACGAAGGA AGCAAATGGT GTTGTGCGAC TTGAAGGCTG GATGAAAATT 
CCGAGGAATA ACAAACGTGG CCAGCAAGGA TGGGACAGGA AATATGTGGT CTTGGAAGGG 
TCTAAAGTGC TGATCTATGA TACTGAATTA AAAGAAGGAG GACAACGGCC TTCAGAGGAG 
TTTGAGCTTT GTCTTCCTGA TGGAGATGTA ACCGTTCATG GTGCCGTTGG ATCTACAGAG 
CTTGTAAATA CTGCAAAATC AGATGTGCCA TACGTCATGA AGATTGAGTC TCATCCACAC 
ACAACTTGTT GGCCAGGGCG CACTATGTAC TTGTTAGCGC CAAGTTTTCC TGATAAGCAG 
CGTTGGGTTA ATGCACTGGA GTCTGTGGTT GCAGGAGGAA GGGTGTCCAG AGAAAAAGCA 
GAGGCTGATG CTAAACTGCT TGGAAACTCT TTGTTAAAAT TGGAGGGGGA AGACCGTCTT 
GATATAAATT GCACAATACC TCTAACTGAG CAGATTGTTT TGGTTGGCGC AGAGGAGGGT 
CTTTATGCTC TGAATGTGCT GAAGAATTCT CTCACTCACA TACCAGGTAT AGGAGCAGTA 
TTCCAGATTC ATATTGTTAA GGAGCTGGAT AAACTTCTTT TAATAGCAGG GGAAGAAAGG 
GCTCTATGCC TTGTTGATGT GAAGAAGGTG AAGCAGTCAT TGGCTCAGTC ACATCTACCA 
GCACAGCCAG AAATCTCAAT GACTGTTTTT GAAGCTGTTA AAGGATGTCA CCTTTTTGCT 
GCAGGAAAGA TTGAAAATGG GTTATGCATC TGTGCAGCCA TGTTAAATAA AGTTGTCATA 
CTGCGCTACA ATGAAAGTCT CGGCAAGTTT TGCATAAGAA AGGAAATTGA AACTTCTGAG 
CCATGCAGTT GCATTTATTT CACTGGCTAC AGTGTCATCA TTGGAACAAA CAAGTTCTAT 
GAGATTGAGA TGAAGCAGTA CACTCTGGAG GAGTTCTTGG ATAAAAATGA CCATTCCTTG 
GCTTCTGCTG TGTTTGCTTC ATCTTCCAAT AGCTTCCCAA TAGCTATAAT GCAAGTAAAC 
AGCACAGGCC AGAGAGAAGA ATACCTCCTT TGCTTCCATG AGTTCGGTGT ATTTGTTGAT 
TCTTATGGAA GACGTAGTCG TACTGAAGAT TTGAAATGGA GTCGGCTTCC TCTTGCATTT 
GCCTACAGGG AACCGTACTT ATTTGTGACC CATTTTAACT CTTTGGAGGT GATTGAGATC 
CAGGGACGTG CTGCACTAGG TGCTCCAGCT CGAGCCCATC TTGATATTCC CAACCCACGT 
TACCTGGGAC CAGCAATCTC TTCTGGAGCC ATTTATATTG CATCTGTTTA CCAGGATAAG 
CTGAGAGTAA TCTGTTGCAA GGGAAATCTA GTAAAGGACA CTACAAGTGA ACTTCATAGG 
GCTCCTTCTA CAACTCGCAG CCCAAACAAG AGAGGACCAC CATCTTACAA TGACCATATA 
ACAAAGCGAA TAGCATCCAG CCCAGTGCCA ACTGAGGTCC AAGGTCTCGT ACGGGAGCCT 
AGTACACCTC ATAGATACCG CGAAGGACGG ACAGAAATGC GCAGAGACAA ATCTCCTGGG 
AGGCCTTTGG ACAGGGAGAA GTCTCCAGGG AGGTTACTGA GCACCAGACG GGAGAGGTCA 
CCTGGAAGGC TTTTTGAAGA GAGCAACAGG AGCCGTGGAG CTGTAAGGAC ACCCTTGTCA 
CAAGTCAATA AGGTTTGGGA CCAGTCTTCT GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751694.1
CDS	3..6218
Translation	MLKFKFGSRSAADAISSDPITSRAARLNNFFQGKSSCQFPFQQQLNVLSREGLLDSLCALYEECCNPALMKIKHVSNFVKKYSDTVAELRELQPNVKDFEIKNVVGRGHFAEVHVVKEKVTGDIFAMKVMKKKALLAQDQVSFFEEERNILSHHNSPWIPQLHYAFQDKENLYLVMQYQPGGDLFALMNRYDEQFDENMAQFYLAELVLAIYSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKLTANKTVNAKLPVGTPDYIAPEVLMVMNGDGRSTYGPECDWWSLGIVAYEMMYGKSPFTEGTSIKTFNNIMNFQRFLKFPEEPKLSSNFLDLIQSLICGQKERLKYEDLCCHPFFSNTDWDRLRNTPPPFVPTLKSDDDTSNFDEPEKNLRILPSSCQLNSAGFSGDDLPFVGFSFIKALSLLNKSESVVSGLDSPAKVSSMEKKLFHKSKELQDTQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLNDIREQSRKLQDIKEQEFQAQLEEMKMMMHQLEEDLLAARRRSDLYETELRDSRLAAEDFKRKAVECQQKLQKAKDHGKGEVGELHTKLEKVNADQQNKIQELQEKLAKAVKASSEATELLQNIRQAKERAEKELEKLKNRDDSNDAIKKKLLDAEERRHSLENQVRRLETVERRENKLKEDIQTKSEQIQQMAEKIMELEERYREAQVGAQRLEVHLKQNEQVYEEKARNIETQMKKDLADKEALELSRIKNEEEICEKNRILSEQKAMINALESKITSLEQRLAELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKIEAQNQKLEEQLERINNQDHTDKNRLLELETRLREISLEHEEQKLELKRQLTEVQLTLQERETQISSLQSARSALESQLHQAKTELEDTTAEAEEEIQVLRSHRDEIQRKFDTLRDSCTVLTELEDQLNRISQENADLNNQNFYLSKQLSEVSGATDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWESWRSSLEDEKTQFECRLREVQRLLDTEKQSRVRSDQRTTESRQMVELAAKEHKAEILALQQALKDQKLKTESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENMQALFSHEKVKMEGTISQQTKLIDFLQAKMDQPSKKKKVQNGLFSRRRDEPNQTSQVPLQYNELKVALEKEKARTAELEEALQKTRIELRSAREEAAHLKVSDHPVPSTPGTARQQIIMSALVRSPEHQPNAVSLLAPQNSRRKESSAPEEFSRRLKERMHHNIPHRFNVALTMRAAKCSVCLDTVHFGRQGAKCLECQVMCHPKCSSCLPATCGLPAEYASHFTEAFCREKMNSPGMQTKEANGVVRLEGWMKIPRNNKRGQQGWDRKYVVLEGSKVLIYDTELKEGGQRPSEEFELCLPDGDVTVHGAVGSTELVNTAKSDVPYVMKIESHPHTTCWPGRTMYLLAPSFPDKQRWVNALESVVAGGRVSREKAEADAKLLGNSLLKLEGEDRLDINCTIPLTEQIVLVGAEEGLYALNVLKNSLTHIPGIGAVFQIHIVKELDKLLLIAGEERALCLVDVKKVKQSLAQSHLPAQPEISMTVFEAVKGCHLFAAGKIENGLCICAAMLNKVVILRYNESLGKFCIRKEIETSEPCSCIYFTGYSVIIGTNKFYEIEMKQYTLEEFLDKNDHSLASAVFASSSNSFPIAIMQVNSTGQREEYLLCFHEFGVFVDSYGRRSRTEDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQGRAALGAPARAHLDIPNPRYLGPAISSGAIYIASVYQDKLRVICCKGNLVKDTTSELHRAPSTTRSPNKRGPPSYNDHITKRIASSPVPTEVQGLVREPSTPHRYREGRTEMRRDKSPGRPLDREKSPGRLLSTRRERSPGRLFEESNRSRGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_031895834.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895834.1

ORF Insert Sequence:

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCTAAA TGACTTGGAA AAGAAGCATG CAATGCTGGA AATGAATGCT 
CGCAGTTTGC AACAAAAACT GGAGACTGAA AGAGAACTAA AGCAACGGCT TTTAGAAGAG 
CAGGCTAAGT TGCAACAGCA GATGGACCTG CAAAAGAATC ACATTTTCCG CCTGACCCAA 
GGACTTCAGG AGGCCCTGGA TCGAGCTGAT CTGCTTAAAA CAGAAAGAAG TGATTTGGAG 
TATCAACTGG AAAACATGCA GGCACTCTTT TCTCATGAAA AAGTAAAGAT GGAAGGAACC 
ATTTCCCAAC AAACAAAACT CATTGACTTC TTACAAGCAA AAATGGATCA GCCATCTAAG 
AAGAAAAAGG TACAAAATGG TTTGTTCAGT AGGCGCAGAG ATGAACCCAA TCAAACCTCA 
CAGGTTCCAT TACAGTACAA TGAGCTAAAA GTAGCGCTTG AAAAAGAGAA GGCTAGAACT 
GCAGAATTAG AAGAGGCTCT TCAAAAAACA CGAATTGAAC TCCGCTCTGC AAGAGAGGAA 
GCTGCTCACT TAAAGGTGTC AGATCATCCT GTTCCTTCTA CACCTGGTAC AGCAAGACAA 
CAGATTATCA TGTCTGCACT GGTCAGATCA CCTGAGCATC AGCCCAATGC AGTCAGCCTG 
CTTGCCCCAC AGAACAGCAG AAGGAAAGAA TCATCAGCTC CTGAAGAATT CAGTCGCCGA 
CTTAAAGAAA GAATGCATCA TAATATTCCA CACAGATTTA ATGTTGCACT TACCATGAGA 
GCAGCAAAAT GTTCGGTGTG CTTGGATACT GTTCACTTTG GGCGGCAGGG AGCAAAGTGC 
CTAGAATGTC AGGTCATGTG TCACCCAAAG TGTTCCTCCT GCCTCCCAGC TACATGTGGC 
CTCCCTGCAG AATATGCTTC ACATTTCACT GAAGCCTTTT GTAGAGAGAA AATGAATTCG 
CCTGGAATGC AGACGAAGGA AGCAAATGGT GTTGTGCGAC TTGAAGGCTG GATGAAAATT 
CCGAGGAATA ACAAACGTGG CCAGCAAGGA TGGGACAGGA AATATGTGGT CTTGGAAGGG 
TCTAAAGTGC TGATCTATGA TACTGAATTA AAAGAAGGAG GACAACGGCC TTCAGAGGAG 
TTTGAGCTTT GTCTTCCTGA TGGAGATGTA ACCGTTCATG GTGCCGTTGG ATCTACAGAG 
CTTGTAAATA CTGCAAAATC AGATGTGCCA TACGTCATGA AGATTGAGTC TCATCCACAC 
ACAACTTGTT GGCCAGGGCG CACTATGTAC TTGTTAGCGC CAAGTTTTCC TGATAAGCAG 
CGTTGGGTTA ATGCACTGGA GTCTGTGGTT GCAGGAGGAA GGGTGTCCAG AGAAAAAGCA 
GAGGCTGATG CTAAACTGCT TGGAAACTCT TTGTTAAAAT TGGAGGGGGA AGACCGTCTT 
GATATAAATT GCACAATACC TCTAACTGAG CAGATTGTTT TGGTTGGCGC AGAGGAGGGT 
CTTTATGCTC TGAATGTGCT GAAGAATTCT CTCACTCACA TACCAGGTAT AGGAGCAGTA 
TTCCAGATTC ATATTGTTAA GGAGCTGGAT AAACTTCTTT TAATAGCAGG GGAAGAAAGG 
GCTCTATGCC TTGTTGATGT GAAGAAGGTG AAGCAGTCAT TGGCTCAGTC ACATCTACCA 
GCACAGCCAG AAATCTCAAT GACTGTTTTT GAAGCTGTTA AAGGATGTCA CCTTTTTGCT 
GCAGGAAAGA TTGAAAATGG GTTATGCATC TGTGCAGCCA TGTTAAATAA AGTTGTCATA 
CTGCGCTACA ATGAAAGTCT CGGCAAGTTT TGCATAAGAA AGGAAATTGA AACTTCTGAG 
CCATGCAGTT GCATTTATTT CACTGGCTAC AGTGTCATCA TTGGAACAAA CAAGTTCTAT 
GAGATTGAGA TGAAGCAGTA CACTCTGGAG GAGTTCTTGG ATAAAAATGA CCATTCCTTG 
GCTTCTGCTG TGTTTGCTTC ATCTTCCAAT AGCTTCCCAA TAGCTATAAT GCAAGTAAAC 
AGCACAGGCC AGAGAGAAGA ATACCTCCTT TGCTTCCATG AGTTCGGTGT ATTTGTTGAT 
TCTTATGGAA GACGTAGTCG TACTGAAGAT TTGAAATGGA GTCGGCTTCC TCTTGCATTT 
GCCTACAGGG AACCGTACTT ATTTGTGACC CATTTTAACT CTTTGGAGGT GATTGAGATC 
CAGGGACGTG CTGCACTAGG TGCTCCAGCT CGAGCCCATC TTGATATTCC CAACCCACGT 
TACCTGGGAC CAGCAATCTC TTCTGGAGCC ATTTATATTG CATCTGTTTA CCAGGATAAG 
CTGAGAGTAA TCTGTTGCAA GGGAAATCTA GTAAAGGACA CTACAAGTGA ACTTCATAGG 
GCTCCTTCTA CAACTCGCAG CCCAAACAAG AGAGGACCAC CATCTTACAA TGACCATATA 
ACAAAGCGAA TAGCATCCAG CCCAGTGCCA ACTGAGGTCC AAGGTCTCGT ACGGGAGCCT 
AGTACACCTC ATAGATACCG CGAAGGACGG ACAGAAATGC GCAGAGACAA ATCTCCTGGG 
AGGCCTTTGG ACAGGGAGAA GTCTCCAGGG AGGTTACTGA GCACCAGACG GGAGAGGTCA 
CCTGGAAGGC TTTTTGAAGA GAGCAACAGG AGCCGTGGAG CTGTAAGGAC ACCCTTGTCA 
CAAGTCAATA AGGTTTGGGA CCAGTCTTCT GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cit ( XM_031895838.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751698.1 Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X3, mRNA.

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CloneID	OXa47602
Clone ID Related Accession (Same CDS sequence)	XM_031895838.1
Accession Version	XM_031895838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	citron Rho-interacting kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGAAAGC CCAAGAGGAG ATGATTTCAG AACTCAGGCA GCAAAAGTTT 
TACCTGGAAA CACAGGCAGG AAAAATAGAG GCTCAGAATC AGAAACTGGA GGAACAGCTA 
GAGAGAATAA ATAATCAGGA CCACACTGAC AAAAACCGCC TTCTAGAACT GGAGACAAGG 
CTGCGAGAGA TTAGTTTGGA GCATGAAGAA CAAAAGCTGG AGCTAAAGAG ACAGTTAACA 
GAGGTGCAGC TCACATTACA GGAAAGAGAA ACCCAGATCT CCAGTTTGCA ATCTGCACGT 
TCTGCATTAG AGAGTCAGCT ACATCAGGCA AAAACAGAGC TGGAAGATAC AACTGCAGAG 
GCTGAGGAAG AGATTCAAGT TTTAAGGTCT CACAGAGATG AGATCCAGCG CAAATTTGAT 
ACTTTGCGTG ACAGTTGTAC GGTCCTTACA GAGCTAGAGG ATCAGCTTAA TCGTATCAGT 
CAAGAAAATG CAGATTTGAA CAATCAAAAC TTTTATTTGT CAAAGCAACT TAGTGAGGTT 
TCAGGAGCCA CTGATGAAAT TGTGCAACTG CGAAGTGAAG TGGATCATCT CAGAAGAGAA 
ATCACAGAAA GAGAGATGCA ACTCACAAGT CAAAAGCAGA CCATGGAGGC CCTGAAAACT 
ACATGTACCA TGCTAGAGGA ACAAGTGCTG GATCTTGAAG CATTGAATGA TGAGTTGCTA 
GAAAAGGAAC GTCAGTGGGA GAGCTGGCGT AGTTCCCTAG AAGATGAGAA AACTCAGTTT 
GAATGTAGAC TTCGAGAGGT ACAGAGATTG CTGGATACCG AAAAACAGAG CAGGGTACGA 
TCTGACCAAC GAACTACAGA ATCACGCCAG ATGGTGGAAT TAGCTGCCAA GGAACACAAG 
GCTGAAATCT TAGCTTTACA GCAAGCACTG AAAGATCAGA AGCTCAAGAC AGAAAGTCTT 
TCAGATAAGC CTGCCCGTAT TTTGAATCAG CTAAATGACT TGGAAAAGAA GCATGCAATG 
CTGGAAATGA ATGCTCGCAG TTTGCAACAA AAACTGGAGA CTGAAAGAGA ACTAAAGCAA 
CGGCTTTTAG AAGAGCAGGC TAAGTTGCAA CAGCAGATGG ACCTGCAAAA GAATCACATT 
TTCCGCCTGA CCCAAGGACT TCAGGAGGCC CTGGATCGAG CTGATCTGCT TAAAACAGAA 
AGAAGTGATT TGGAGTATCA ACTGGAAAAC ATGCAGGCAC TCTTTTCTCA TGAAAAAGTA 
AAGATGGAAG GAACCATTTC CCAACAAACA AAACTCATTG ACTTCTTACA AGCAAAAATG 
GATCAGCCAT CTAAGAAGAA AAAGGTACAA AATGGTTTGT TCAGTAGGCG CAGAGATGAA 
CCCAATCAAA CCTCACAGGT TCCATTACAG TACAATGAGC TAAAAGTAGC GCTTGAAAAA 
GAGAAGGCTA GAACTGCAGA ATTAGAAGAG GCTCTTCAAA AAACACGAAT TGAACTCCGC 
TCTGCAAGAG AGGAAGCTGC TCACTTAAAG GTGTCAGATC ATCCTGTTCC TTCTACACCT 
GGTACAGCAA GACAACAGAT TATCATGTCT GCACTGGTCA GATCACCTGA GCATCAGCCC 
AATGCAGTCA GCCTGCTTGC CCCACAGAAC AGCAGAAGGA AAGAATCATC AGCTCCTGAA 
GAATTCAGTC GCCGACTTAA AGAAAGAATG CATCATAATA TTCCACACAG ATTTAATGTT 
GCACTTACCA TGAGAGCAGC AAAATGTTCG GTGTGCTTGG ATACTGTTCA CTTTGGGCGG 
CAGGGAGCAA AGTGCCTAGA ATGTCAGGTC ATGTGTCACC CAAAGTGTTC CTCCTGCCTC 
CCAGCTACAT GTGGCCTCCC TGCAGAATAT GCTTCACATT TCACTGAAGC CTTTTGTAGA 
GAGAAAATGA ATTCGCCTGG AATGCAGACG AAGGAAGCAA ATGGTGTTGT GCGACTTGAA 
GGCTGGATGA AAATTCCGAG GAATAACAAA CGTGGCCAGC AAGGATGGGA CAGGAAATAT 
GTGGTCTTGG AAGGGTCTAA AGTGCTGATC TATGATACTG AATTAAAAGA AGGAGGACAA 
CGGCCTTCAG AGGAGTTTGA GCTTTGTCTT CCTGATGGAG ATGTAACCGT TCATGGTGCC 
GTTGGATCTA CAGAGCTTGT AAATACTGCA AAATCAGATG TGCCATACGT CATGAAGATT 
GAGTCTCATC CACACACAAC TTGTTGGCCA GGGCGCACTA TGTACTTGTT AGCGCCAAGT 
TTTCCTGATA AGCAGCGTTG GGTTAATGCA CTGGAGTCTG TGGTTGCAGG AGGAAGGGTG 
TCCAGAGAAA AAGCAGAGGC TGATGCTAAA CTGCTTGGAA ACTCTTTGTT AAAATTGGAG 
GGGGAAGACC GTCTTGATAT AAATTGCACA ATACCTCTAA CTGAGCAGAT TGTTTTGGTT 
GGCGCAGAGG AGGGTCTTTA TGCTCTGAAT GTGCTGAAGA ATTCTCTCAC TCACATACCA 
GGTATAGGAG CAGTATTCCA GATTCATATT GTTAAGGAGC TGGATAAACT TCTTTTAATA 
GCAGGGGAAG AAAGGGCTCT ATGCCTTGTT GATGTGAAGA AGGTGAAGCA GTCATTGGCT 
CAGTCACATC TACCAGCACA GCCAGAAATC TCAATGACTG TTTTTGAAGC TGTTAAAGGA 
TGTCACCTTT TTGCTGCAGG AAAGATTGAA AATGGGTTAT GCATCTGTGC AGCCATGTTA 
AATAAAGTTG TCATACTGCG CTACAATGAA AGTCTCGGCA AGTTTTGCAT AAGAAAGGAA 
ATTGAAACTT CTGAGCCATG CAGTTGCATT TATTTCACTG GCTACAGTGT CATCATTGGA 
ACAAACAAGT TCTATGAGAT TGAGATGAAG CAGTACACTC TGGAGGAGTT CTTGGATAAA 
AATGACCATT CCTTGGCTTC TGCTGTGTTT GCTTCATCTT CCAATAGCTT CCCAATAGCT 
ATAATGCAAG TAAACAGCAC AGGCCAGAGA GAAGAATACC TCCTTTGCTT CCATGAGTTC 
GGTGTATTTG TTGATTCTTA TGGAAGACGT AGTCGTACTG AAGATTTGAA ATGGAGTCGG 
CTTCCTCTTG CATTTGCCTA CAGGGAACCG TACTTATTTG TGACCCATTT TAACTCTTTG 
GAGGTGATTG AGATCCAGGG ACGTGCTGCA CTAGGTGCTC CAGCTCGAGC CCATCTTGAT 
ATTCCCAACC CACGTTACCT GGGACCAGCA ATCTCTTCTG GAGCCATTTA TATTGCATCT 
GTTTACCAGG ATAAGCTGAG AGTAATCTGT TGCAAGGGAA ATCTAGTAAA GGACACTACA 
AGTGAACTTC ATAGGGCTCC TTCTACAACT CGCAGCCCAA ACAAGAGAGG ACCACCATCT 
TACAATGACC ATATAACAAA GCGAATAGCA TCCAGCCCAG TGCCAACTGA GGTCCAAGGT 
CTCGTACGGG AGCCTAGTAC ACCTCATAGA TACCGCGAAG GACGGACAGA AATGCGCAGA 
GACAAATCTC CTGGGAGGCC TTTGGACAGG GAGAAGTCTC CAGGGAGGTT ACTGAGCACC 
AGACGGGAGA GGTCACCTGG AAGGCTTTTT GAAGAGAGCA ACAGGAGCCG TGGAGCTGTA 
AGGACACCCT TGTCACAAGT CAATAAGGTT TGGGACCAGT CTTCTGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751698.1
CDS	3..6173
Translation	MLKFKFGSRSAADAISSDPITSRAARLNNFFQGKSSCQFPFQQQLNVLSREGLLDSLCALYEECCNPALMKIKHVSNFVKKYSDTVAELRELQPNVKDFEIKNVVGRGHFAEVHVVKEKVTGDIFAMKVMKKKALLAQDQVSFFEEERNILSHHNSPWIPQLHYAFQDKENLYLVMQYQPGGDLFALMNRYDEQFDENMAQFYLAELVLAIYSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKLTANKTVNAKLPVGTPDYIAPEVLMVMNGDGRSTYGPECDWWSLGIVAYEMMYGKSPFTEGTSIKTFNNIMNFQRFLKFPEEPKLSSNFLDLIQSLICGQKERLKYEDLCCHPFFSNTDWDRLRNTPPPFVPTLKSDDDTSNFDEPEKNLRILPSSCQLNSAGFSGDDLPFVGFSFIKALSLLNKSESVVSGLDSPAKVSSMEKKLFHKSKELQDTQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLNDIREQSRKLQDIKEQEFQAQLEEMKMMMHQLEEDLLAARRRSDLYETELRDSRLAAEDFKRKAVECQQKLQKAKDHGKGEVGELHTKLEKVNADQQNKIQELQEKLAKAVKASSEATELLQNIRQAKERAEKELEKLKNRDDSNDAIKKKLLDAEERRHSLENQVRRLETVERRENKLKEDIQTKSEQIQQMAEKIMELEERYREAQVGAQRLEVHLKQNEQVYEEKARNIETQMKKDLADKEALELSRIKNEEEICEKNRILSEQKAMINALESKITSLEQRKAQEEMISELRQQKFYLETQAGKIEAQNQKLEEQLERINNQDHTDKNRLLELETRLREISLEHEEQKLELKRQLTEVQLTLQERETQISSLQSARSALESQLHQAKTELEDTTAEAEEEIQVLRSHRDEIQRKFDTLRDSCTVLTELEDQLNRISQENADLNNQNFYLSKQLSEVSGATDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWESWRSSLEDEKTQFECRLREVQRLLDTEKQSRVRSDQRTTESRQMVELAAKEHKAEILALQQALKDQKLKTESLSDKPARILNQLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENMQALFSHEKVKMEGTISQQTKLIDFLQAKMDQPSKKKKVQNGLFSRRRDEPNQTSQVPLQYNELKVALEKEKARTAELEEALQKTRIELRSAREEAAHLKVSDHPVPSTPGTARQQIIMSALVRSPEHQPNAVSLLAPQNSRRKESSAPEEFSRRLKERMHHNIPHRFNVALTMRAAKCSVCLDTVHFGRQGAKCLECQVMCHPKCSSCLPATCGLPAEYASHFTEAFCREKMNSPGMQTKEANGVVRLEGWMKIPRNNKRGQQGWDRKYVVLEGSKVLIYDTELKEGGQRPSEEFELCLPDGDVTVHGAVGSTELVNTAKSDVPYVMKIESHPHTTCWPGRTMYLLAPSFPDKQRWVNALESVVAGGRVSREKAEADAKLLGNSLLKLEGEDRLDINCTIPLTEQIVLVGAEEGLYALNVLKNSLTHIPGIGAVFQIHIVKELDKLLLIAGEERALCLVDVKKVKQSLAQSHLPAQPEISMTVFEAVKGCHLFAAGKIENGLCICAAMLNKVVILRYNESLGKFCIRKEIETSEPCSCIYFTGYSVIIGTNKFYEIEMKQYTLEEFLDKNDHSLASAVFASSSNSFPIAIMQVNSTGQREEYLLCFHEFGVFVDSYGRRSRTEDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQGRAALGAPARAHLDIPNPRYLGPAISSGAIYIASVYQDKLRVICCKGNLVKDTTSELHRAPSTTRSPNKRGPPSYNDHITKRIASSPVPTEVQGLVREPSTPHRYREGRTEMRRDKSPGRPLDREKSPGRLLSTRRERSPGRLFEESNRSRGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_031895838.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895838.1

ORF Insert Sequence:

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGAAAGC CCAAGAGGAG ATGATTTCAG AACTCAGGCA GCAAAAGTTT 
TACCTGGAAA CACAGGCAGG AAAAATAGAG GCTCAGAATC AGAAACTGGA GGAACAGCTA 
GAGAGAATAA ATAATCAGGA CCACACTGAC AAAAACCGCC TTCTAGAACT GGAGACAAGG 
CTGCGAGAGA TTAGTTTGGA GCATGAAGAA CAAAAGCTGG AGCTAAAGAG ACAGTTAACA 
GAGGTGCAGC TCACATTACA GGAAAGAGAA ACCCAGATCT CCAGTTTGCA ATCTGCACGT 
TCTGCATTAG AGAGTCAGCT ACATCAGGCA AAAACAGAGC TGGAAGATAC AACTGCAGAG 
GCTGAGGAAG AGATTCAAGT TTTAAGGTCT CACAGAGATG AGATCCAGCG CAAATTTGAT 
ACTTTGCGTG ACAGTTGTAC GGTCCTTACA GAGCTAGAGG ATCAGCTTAA TCGTATCAGT 
CAAGAAAATG CAGATTTGAA CAATCAAAAC TTTTATTTGT CAAAGCAACT TAGTGAGGTT 
TCAGGAGCCA CTGATGAAAT TGTGCAACTG CGAAGTGAAG TGGATCATCT CAGAAGAGAA 
ATCACAGAAA GAGAGATGCA ACTCACAAGT CAAAAGCAGA CCATGGAGGC CCTGAAAACT 
ACATGTACCA TGCTAGAGGA ACAAGTGCTG GATCTTGAAG CATTGAATGA TGAGTTGCTA 
GAAAAGGAAC GTCAGTGGGA GAGCTGGCGT AGTTCCCTAG AAGATGAGAA AACTCAGTTT 
GAATGTAGAC TTCGAGAGGT ACAGAGATTG CTGGATACCG AAAAACAGAG CAGGGTACGA 
TCTGACCAAC GAACTACAGA ATCACGCCAG ATGGTGGAAT TAGCTGCCAA GGAACACAAG 
GCTGAAATCT TAGCTTTACA GCAAGCACTG AAAGATCAGA AGCTCAAGAC AGAAAGTCTT 
TCAGATAAGC CTGCCCGTAT TTTGAATCAG CTAAATGACT TGGAAAAGAA GCATGCAATG 
CTGGAAATGA ATGCTCGCAG TTTGCAACAA AAACTGGAGA CTGAAAGAGA ACTAAAGCAA 
CGGCTTTTAG AAGAGCAGGC TAAGTTGCAA CAGCAGATGG ACCTGCAAAA GAATCACATT 
TTCCGCCTGA CCCAAGGACT TCAGGAGGCC CTGGATCGAG CTGATCTGCT TAAAACAGAA 
AGAAGTGATT TGGAGTATCA ACTGGAAAAC ATGCAGGCAC TCTTTTCTCA TGAAAAAGTA 
AAGATGGAAG GAACCATTTC CCAACAAACA AAACTCATTG ACTTCTTACA AGCAAAAATG 
GATCAGCCAT CTAAGAAGAA AAAGGTACAA AATGGTTTGT TCAGTAGGCG CAGAGATGAA 
CCCAATCAAA CCTCACAGGT TCCATTACAG TACAATGAGC TAAAAGTAGC GCTTGAAAAA 
GAGAAGGCTA GAACTGCAGA ATTAGAAGAG GCTCTTCAAA AAACACGAAT TGAACTCCGC 
TCTGCAAGAG AGGAAGCTGC TCACTTAAAG GTGTCAGATC ATCCTGTTCC TTCTACACCT 
GGTACAGCAA GACAACAGAT TATCATGTCT GCACTGGTCA GATCACCTGA GCATCAGCCC 
AATGCAGTCA GCCTGCTTGC CCCACAGAAC AGCAGAAGGA AAGAATCATC AGCTCCTGAA 
GAATTCAGTC GCCGACTTAA AGAAAGAATG CATCATAATA TTCCACACAG ATTTAATGTT 
GCACTTACCA TGAGAGCAGC AAAATGTTCG GTGTGCTTGG ATACTGTTCA CTTTGGGCGG 
CAGGGAGCAA AGTGCCTAGA ATGTCAGGTC ATGTGTCACC CAAAGTGTTC CTCCTGCCTC 
CCAGCTACAT GTGGCCTCCC TGCAGAATAT GCTTCACATT TCACTGAAGC CTTTTGTAGA 
GAGAAAATGA ATTCGCCTGG AATGCAGACG AAGGAAGCAA ATGGTGTTGT GCGACTTGAA 
GGCTGGATGA AAATTCCGAG GAATAACAAA CGTGGCCAGC AAGGATGGGA CAGGAAATAT 
GTGGTCTTGG AAGGGTCTAA AGTGCTGATC TATGATACTG AATTAAAAGA AGGAGGACAA 
CGGCCTTCAG AGGAGTTTGA GCTTTGTCTT CCTGATGGAG ATGTAACCGT TCATGGTGCC 
GTTGGATCTA CAGAGCTTGT AAATACTGCA AAATCAGATG TGCCATACGT CATGAAGATT 
GAGTCTCATC CACACACAAC TTGTTGGCCA GGGCGCACTA TGTACTTGTT AGCGCCAAGT 
TTTCCTGATA AGCAGCGTTG GGTTAATGCA CTGGAGTCTG TGGTTGCAGG AGGAAGGGTG 
TCCAGAGAAA AAGCAGAGGC TGATGCTAAA CTGCTTGGAA ACTCTTTGTT AAAATTGGAG 
GGGGAAGACC GTCTTGATAT AAATTGCACA ATACCTCTAA CTGAGCAGAT TGTTTTGGTT 
GGCGCAGAGG AGGGTCTTTA TGCTCTGAAT GTGCTGAAGA ATTCTCTCAC TCACATACCA 
GGTATAGGAG CAGTATTCCA GATTCATATT GTTAAGGAGC TGGATAAACT TCTTTTAATA 
GCAGGGGAAG AAAGGGCTCT ATGCCTTGTT GATGTGAAGA AGGTGAAGCA GTCATTGGCT 
CAGTCACATC TACCAGCACA GCCAGAAATC TCAATGACTG TTTTTGAAGC TGTTAAAGGA 
TGTCACCTTT TTGCTGCAGG AAAGATTGAA AATGGGTTAT GCATCTGTGC AGCCATGTTA 
AATAAAGTTG TCATACTGCG CTACAATGAA AGTCTCGGCA AGTTTTGCAT AAGAAAGGAA 
ATTGAAACTT CTGAGCCATG CAGTTGCATT TATTTCACTG GCTACAGTGT CATCATTGGA 
ACAAACAAGT TCTATGAGAT TGAGATGAAG CAGTACACTC TGGAGGAGTT CTTGGATAAA 
AATGACCATT CCTTGGCTTC TGCTGTGTTT GCTTCATCTT CCAATAGCTT CCCAATAGCT 
ATAATGCAAG TAAACAGCAC AGGCCAGAGA GAAGAATACC TCCTTTGCTT CCATGAGTTC 
GGTGTATTTG TTGATTCTTA TGGAAGACGT AGTCGTACTG AAGATTTGAA ATGGAGTCGG 
CTTCCTCTTG CATTTGCCTA CAGGGAACCG TACTTATTTG TGACCCATTT TAACTCTTTG 
GAGGTGATTG AGATCCAGGG ACGTGCTGCA CTAGGTGCTC CAGCTCGAGC CCATCTTGAT 
ATTCCCAACC CACGTTACCT GGGACCAGCA ATCTCTTCTG GAGCCATTTA TATTGCATCT 
GTTTACCAGG ATAAGCTGAG AGTAATCTGT TGCAAGGGAA ATCTAGTAAA GGACACTACA 
AGTGAACTTC ATAGGGCTCC TTCTACAACT CGCAGCCCAA ACAAGAGAGG ACCACCATCT 
TACAATGACC ATATAACAAA GCGAATAGCA TCCAGCCCAG TGCCAACTGA GGTCCAAGGT 
CTCGTACGGG AGCCTAGTAC ACCTCATAGA TACCGCGAAG GACGGACAGA AATGCGCAGA 
GACAAATCTC CTGGGAGGCC TTTGGACAGG GAGAAGTCTC CAGGGAGGTT ACTGAGCACC 
AGACGGGAGA GGTCACCTGG AAGGCTTTTT GAAGAGAGCA ACAGGAGCCG TGGAGCTGTA 
AGGACACCCT TGTCACAAGT CAATAAGGTT TGGGACCAGT CTTCTGTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cit ( XM_031895842.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751702.1 Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X4, mRNA.

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CloneID	OXa47603
Clone ID Related Accession (Same CDS sequence)	XM_031895842.1 , XM_031895845.1
Accession Version	XM_031895842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	citron Rho-interacting kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCCTGC CCGTATTTTG AATCAGCTAA ATGACTTGGA AAAGAAGCAT 
GCAATGCTGG AAATGAATGC TCGCAGTTTG CAACAAAAAC TGGAGACTGA AAGAGAACTA 
AAGCAACGGC TTTTAGAAGA GCAGGCTAAG TTGCAACAGC AGATGGACCT GCAAAAGAAT 
CACATTTTCC GCCTGACCCA AGGACTTCAG GAGGCCCTGG ATCGAGCTGA TCTGCTTAAA 
ACAGAAAGAA GTGATTTGGA GTATCAACTG GAAAACATGC AGGCACTCTT TTCTCATGAA 
AAAGTAAAGA TGGAAGGAAC CATTTCCCAA CAAACAAAAC TCATTGACTT CTTACAAGCA 
AAAATGGATC AGCCATCTAA GAAGAAAAAG GTACAAAATG GTTTGTTCAG TAGGCGCAGA 
GATGAACCCA ATCAAACCTC ACAGGTTCCA TTACAGTACA ATGAGCTAAA AGTAGCGCTT 
GAAAAAGAGA AGGCTAGAAC TGCAGAATTA GAAGAGGCTC TTCAAAAAAC ACGAATTGAA 
CTCCGCTCTG CAAGAGAGGA AGCTGCTCAC TTAAAGGTGT CAGATCATCC TGTTCCTTCT 
ACACCTGGTA CAGCAAGACA ACAGATTATC ATGTCTGCAC TGGTCAGATC ACCTGAGCAT 
CAGCCCAATG CAGTCAGCCT GCTTGCCCCA CAGAACAGCA GAAGGAAAGA ATCATCAGCT 
CCTGAAGAAT TCAGTCGCCG ACTTAAAGAA AGAATGCATC ATAATATTCC ACACAGATTT 
AATGTTGCAC TTACCATGAG AGCAGCAAAA TGTTCGGTGT GCTTGGATAC TGTTCACTTT 
GGGCGGCAGG GAGCAAAGTG CCTAGAATGT CAGGTCATGT GTCACCCAAA GTGTTCCTCC 
TGCCTCCCAG CTACATGTGG CCTCCCTGCA GAATATGCTT CACATTTCAC TGAAGCCTTT 
TGTAGAGAGA AAATGAATTC GCCTGGAATG CAGACGAAGG AAGCAAATGG TGTTGTGCGA 
CTTGAAGGCT GGATGAAAAT TCCGAGGAAT AACAAACGTG GCCAGCAAGG ATGGGACAGG 
AAATATGTGG TCTTGGAAGG GTCTAAAGTG CTGATCTATG ATACTGAATT AAAAGAAGGA 
GGACAACGGC CTTCAGAGGA GTTTGAGCTT TGTCTTCCTG ATGGAGATGT AACCGTTCAT 
GGTGCCGTTG GATCTACAGA GCTTGTAAAT ACTGCAAAAT CAGATGTGCC ATACGTCATG 
AAGATTGAGT CTCATCCACA CACAACTTGT TGGCCAGGGC GCACTATGTA CTTGTTAGCG 
CCAAGTTTTC CTGATAAGCA GCGTTGGGTT AATGCACTGG AGTCTGTGGT TGCAGGAGGA 
AGGGTGTCCA GAGAAAAAGC AGAGGCTGAT GCTAAACTGC TTGGAAACTC TTTGTTAAAA 
TTGGAGGGGG AAGACCGTCT TGATATAAAT TGCACAATAC CTCTAACTGA GCAGATTGTT 
TTGGTTGGCG CAGAGGAGGG TCTTTATGCT CTGAATGTGC TGAAGAATTC TCTCACTCAC 
ATACCAGGTA TAGGAGCAGT ATTCCAGATT CATATTGTTA AGGAGCTGGA TAAACTTCTT 
TTAATAGCAG GGGAAGAAAG GGCTCTATGC CTTGTTGATG TGAAGAAGGT GAAGCAGTCA 
TTGGCTCAGT CACATCTACC AGCACAGCCA GAAATCTCAA TGACTGTTTT TGAAGCTGTT 
AAAGGATGTC ACCTTTTTGC TGCAGGAAAG ATTGAAAATG GGTTATGCAT CTGTGCAGCC 
ATGTTAAATA AAGTTGTCAT ACTGCGCTAC AATGAAAGTC TCGGCAAGTT TTGCATAAGA 
AAGGAAATTG AAACTTCTGA GCCATGCAGT TGCATTTATT TCACTGGCTA CAGTGTCATC 
ATTGGAACAA ACAAGTTCTA TGAGATTGAG ATGAAGCAGT ACACTCTGGA GGAGTTCTTG 
GATAAAAATG ACCATTCCTT GGCTTCTGCT GTGTTTGCTT CATCTTCCAA TAGCTTCCCA 
ATAGCTATAA TGCAAGTAAA CAGCACAGGC CAGAGAGAAG AATACCTCCT TTGCTTCCAT 
GAGTTCGGTG TATTTGTTGA TTCTTATGGA AGACGTAGTC GTACTGAAGA TTTGAAATGG 
AGTCGGCTTC CTCTTGCATT TGCCTACAGG GAACCGTACT TATTTGTGAC CCATTTTAAC 
TCTTTGGAGG TGATTGAGAT CCAGGGACGT GCTGCACTAG GTGCTCCAGC TCGAGCCCAT 
CTTGATATTC CCAACCCACG TTACCTGGGA CCAGCAATCT CTTCTGGAGC CATTTATATT 
GCATCTGTTT ACCAGGATAA GCTGAGAGTA ATCTGTTGCA AGGGAAATCT AGTAAAGGAC 
ACTACAAGTG AACTTCATAG GGCTCCTTCT ACAACTCGCA GCCCAAACAA GAGAGGACCA 
CCATCTTACA ATGACCATAT AACAAAGCGA ATAGCATCCA GCCCAGTGCC AACTGAGGTC 
CAAGGTCTCG TACGGGAGCC TAGTACACCT CATAGATACC GCGAAGGACG GACAGAAATG 
CGCAGAGACA AATCTCCTGG GAGGCCTTTG GACAGGGAGA AGTCTCCAGG GAGGTTACTG 
AGCACCAGAC GGGAGAGGTC ACCTGGAAGG CTTTTTGAAG AGAGCAACAG GAGCCGTGGA 
GCTGTAAGGA CACCCTTGTC ACAAGTCAAT AAGGTTTGGG ACCAGTCTTC TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751702.1
CDS	877..7113
Translation	MLKFKFGSRSAADAISSDPITSRAARLNNFFQGKSSCQFPFQQQLNVLSREGLLDSLCALYEECCNPALMKIKHVSNFVKKYSDTVAELRELQPNVKDFEIKNVVGRGHFAEVHVVKEKVTGDIFAMKVMKKKALLAQDQVSFFEEERNILSHHNSPWIPQLHYAFQDKENLYLVMQYQPGGDLFALMNRYDEQFDENMAQFYLAELVLAIYSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKLTANKTVNAKLPVGTPDYIAPEVLMVMNGDGRSTYGPECDWWSLGIVAYEMMYGKSPFTEGTSIKTFNNIMNFQRFLKFPEEPKLSSNFLDLIQSLICGQKERLKYEDLCCHPFFSNTDWDRLRNTPPPFVPTLKSDDDTSNFDEPEKNLRILPSSCQLNSAGFSGDDLPFVGFSFIKALSLLNKSESVVSGLDSPAKVSSMEKKLFHKSKELQDTQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLNDIREQSRKLQDIKEQEFQAQLEEMKMMMHQLEEDLLAARRRSDLYETELRDSRLAAEDFKRKAVECQQKLQKAKDHGKGEVGELHTKLEKVNADQQNKIQELQEKLAKAVKASSEATELLQNIRQAKERAEKELEKLKNRDDSNDAIKKKLLDAEERRHSLENQVRRLETVERRENKLKEDIQTKSEQIQQMAEKIMELEERYREAQVGAQRLEVHLKQNEQVYEEKARNIETQMKKDLADKEALELSRIKNEEEICEKNRILSEQKAMINALESKITSLEQRLAELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKIEAQNQKLEEQLERINNQDHTDKNRLLELETRLREISLEHEEQKLELKRQLTEVQLTLQERETQISSLQSARSALESQLHQAKTELEDTTAEAEEEIQVLRSHRDEIQRKFDTLRDSCTVLTELEDQLNRISQENADLNNQNFYLSKQLSEVSGATDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWESWRSSLEDEKTQFECRLREVQRLLDTEKQSRVRSDQRTTESRQMVELAAKEHKAEILALQQALKDQKLKTESLSDKPARILNQLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENMQALFSHEKVKMEGTISQQTKLIDFLQAKMDQPSKKKKVQNGLFSRRRDEPNQTSQVPLQYNELKVALEKEKARTAELEEALQKTRIELRSAREEAAHLKVSDHPVPSTPGTARQQIIMSALVRSPEHQPNAVSLLAPQNSRRKESSAPEEFSRRLKERMHHNIPHRFNVALTMRAAKCSVCLDTVHFGRQGAKCLECQVMCHPKCSSCLPATCGLPAEYASHFTEAFCREKMNSPGMQTKEANGVVRLEGWMKIPRNNKRGQQGWDRKYVVLEGSKVLIYDTELKEGGQRPSEEFELCLPDGDVTVHGAVGSTELVNTAKSDVPYVMKIESHPHTTCWPGRTMYLLAPSFPDKQRWVNALESVVAGGRVSREKAEADAKLLGNSLLKLEGEDRLDINCTIPLTEQIVLVGAEEGLYALNVLKNSLTHIPGIGAVFQIHIVKELDKLLLIAGEERALCLVDVKKVKQSLAQSHLPAQPEISMTVFEAVKGCHLFAAGKIENGLCICAAMLNKVVILRYNESLGKFCIRKEIETSEPCSCIYFTGYSVIIGTNKFYEIEMKQYTLEEFLDKNDHSLASAVFASSSNSFPIAIMQVNSTGQREEYLLCFHEFGVFVDSYGRRSRTEDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQGRAALGAPARAHLDIPNPRYLGPAISSGAIYIASVYQDKLRVICCKGNLVKDTTSELHRAPSTTRSPNKRGPPSYNDHITKRIASSPVPTEVQGLVREPSTPHRYREGRTEMRRDKSPGRPLDREKSPGRLLSTRRERSPGRLFEESNRSRGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_031895842.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895842.1

ORF Insert Sequence:

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCCTGC CCGTATTTTG AATCAGCTAA ATGACTTGGA AAAGAAGCAT 
GCAATGCTGG AAATGAATGC TCGCAGTTTG CAACAAAAAC TGGAGACTGA AAGAGAACTA 
AAGCAACGGC TTTTAGAAGA GCAGGCTAAG TTGCAACAGC AGATGGACCT GCAAAAGAAT 
CACATTTTCC GCCTGACCCA AGGACTTCAG GAGGCCCTGG ATCGAGCTGA TCTGCTTAAA 
ACAGAAAGAA GTGATTTGGA GTATCAACTG GAAAACATGC AGGCACTCTT TTCTCATGAA 
AAAGTAAAGA TGGAAGGAAC CATTTCCCAA CAAACAAAAC TCATTGACTT CTTACAAGCA 
AAAATGGATC AGCCATCTAA GAAGAAAAAG GTACAAAATG GTTTGTTCAG TAGGCGCAGA 
GATGAACCCA ATCAAACCTC ACAGGTTCCA TTACAGTACA ATGAGCTAAA AGTAGCGCTT 
GAAAAAGAGA AGGCTAGAAC TGCAGAATTA GAAGAGGCTC TTCAAAAAAC ACGAATTGAA 
CTCCGCTCTG CAAGAGAGGA AGCTGCTCAC TTAAAGGTGT CAGATCATCC TGTTCCTTCT 
ACACCTGGTA CAGCAAGACA ACAGATTATC ATGTCTGCAC TGGTCAGATC ACCTGAGCAT 
CAGCCCAATG CAGTCAGCCT GCTTGCCCCA CAGAACAGCA GAAGGAAAGA ATCATCAGCT 
CCTGAAGAAT TCAGTCGCCG ACTTAAAGAA AGAATGCATC ATAATATTCC ACACAGATTT 
AATGTTGCAC TTACCATGAG AGCAGCAAAA TGTTCGGTGT GCTTGGATAC TGTTCACTTT 
GGGCGGCAGG GAGCAAAGTG CCTAGAATGT CAGGTCATGT GTCACCCAAA GTGTTCCTCC 
TGCCTCCCAG CTACATGTGG CCTCCCTGCA GAATATGCTT CACATTTCAC TGAAGCCTTT 
TGTAGAGAGA AAATGAATTC GCCTGGAATG CAGACGAAGG AAGCAAATGG TGTTGTGCGA 
CTTGAAGGCT GGATGAAAAT TCCGAGGAAT AACAAACGTG GCCAGCAAGG ATGGGACAGG 
AAATATGTGG TCTTGGAAGG GTCTAAAGTG CTGATCTATG ATACTGAATT AAAAGAAGGA 
GGACAACGGC CTTCAGAGGA GTTTGAGCTT TGTCTTCCTG ATGGAGATGT AACCGTTCAT 
GGTGCCGTTG GATCTACAGA GCTTGTAAAT ACTGCAAAAT CAGATGTGCC ATACGTCATG 
AAGATTGAGT CTCATCCACA CACAACTTGT TGGCCAGGGC GCACTATGTA CTTGTTAGCG 
CCAAGTTTTC CTGATAAGCA GCGTTGGGTT AATGCACTGG AGTCTGTGGT TGCAGGAGGA 
AGGGTGTCCA GAGAAAAAGC AGAGGCTGAT GCTAAACTGC TTGGAAACTC TTTGTTAAAA 
TTGGAGGGGG AAGACCGTCT TGATATAAAT TGCACAATAC CTCTAACTGA GCAGATTGTT 
TTGGTTGGCG CAGAGGAGGG TCTTTATGCT CTGAATGTGC TGAAGAATTC TCTCACTCAC 
ATACCAGGTA TAGGAGCAGT ATTCCAGATT CATATTGTTA AGGAGCTGGA TAAACTTCTT 
TTAATAGCAG GGGAAGAAAG GGCTCTATGC CTTGTTGATG TGAAGAAGGT GAAGCAGTCA 
TTGGCTCAGT CACATCTACC AGCACAGCCA GAAATCTCAA TGACTGTTTT TGAAGCTGTT 
AAAGGATGTC ACCTTTTTGC TGCAGGAAAG ATTGAAAATG GGTTATGCAT CTGTGCAGCC 
ATGTTAAATA AAGTTGTCAT ACTGCGCTAC AATGAAAGTC TCGGCAAGTT TTGCATAAGA 
AAGGAAATTG AAACTTCTGA GCCATGCAGT TGCATTTATT TCACTGGCTA CAGTGTCATC 
ATTGGAACAA ACAAGTTCTA TGAGATTGAG ATGAAGCAGT ACACTCTGGA GGAGTTCTTG 
GATAAAAATG ACCATTCCTT GGCTTCTGCT GTGTTTGCTT CATCTTCCAA TAGCTTCCCA 
ATAGCTATAA TGCAAGTAAA CAGCACAGGC CAGAGAGAAG AATACCTCCT TTGCTTCCAT 
GAGTTCGGTG TATTTGTTGA TTCTTATGGA AGACGTAGTC GTACTGAAGA TTTGAAATGG 
AGTCGGCTTC CTCTTGCATT TGCCTACAGG GAACCGTACT TATTTGTGAC CCATTTTAAC 
TCTTTGGAGG TGATTGAGAT CCAGGGACGT GCTGCACTAG GTGCTCCAGC TCGAGCCCAT 
CTTGATATTC CCAACCCACG TTACCTGGGA CCAGCAATCT CTTCTGGAGC CATTTATATT 
GCATCTGTTT ACCAGGATAA GCTGAGAGTA ATCTGTTGCA AGGGAAATCT AGTAAAGGAC 
ACTACAAGTG AACTTCATAG GGCTCCTTCT ACAACTCGCA GCCCAAACAA GAGAGGACCA 
CCATCTTACA ATGACCATAT AACAAAGCGA ATAGCATCCA GCCCAGTGCC AACTGAGGTC 
CAAGGTCTCG TACGGGAGCC TAGTACACCT CATAGATACC GCGAAGGACG GACAGAAATG 
CGCAGAGACA AATCTCCTGG GAGGCCTTTG GACAGGGAGA AGTCTCCAGG GAGGTTACTG 
AGCACCAGAC GGGAGAGGTC ACCTGGAAGG CTTTTTGAAG AGAGCAACAG GAGCCGTGGA 
GCTGTAAGGA CACCCTTGTC ACAAGTCAAT AAGGTTTGGG ACCAGTCTTC TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cit ( XM_031895845.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751705.1 Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X5, mRNA.

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CloneID	OXa47603
Clone ID Related Accession (Same CDS sequence)	XM_031895842.1 , XM_031895845.1
Accession Version	XM_031895845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	citron Rho-interacting kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCCTGC CCGTATTTTG AATCAGCTAA ATGACTTGGA AAAGAAGCAT 
GCAATGCTGG AAATGAATGC TCGCAGTTTG CAACAAAAAC TGGAGACTGA AAGAGAACTA 
AAGCAACGGC TTTTAGAAGA GCAGGCTAAG TTGCAACAGC AGATGGACCT GCAAAAGAAT 
CACATTTTCC GCCTGACCCA AGGACTTCAG GAGGCCCTGG ATCGAGCTGA TCTGCTTAAA 
ACAGAAAGAA GTGATTTGGA GTATCAACTG GAAAACATGC AGGCACTCTT TTCTCATGAA 
AAAGTAAAGA TGGAAGGAAC CATTTCCCAA CAAACAAAAC TCATTGACTT CTTACAAGCA 
AAAATGGATC AGCCATCTAA GAAGAAAAAG GTACAAAATG GTTTGTTCAG TAGGCGCAGA 
GATGAACCCA ATCAAACCTC ACAGGTTCCA TTACAGTACA ATGAGCTAAA AGTAGCGCTT 
GAAAAAGAGA AGGCTAGAAC TGCAGAATTA GAAGAGGCTC TTCAAAAAAC ACGAATTGAA 
CTCCGCTCTG CAAGAGAGGA AGCTGCTCAC TTAAAGGTGT CAGATCATCC TGTTCCTTCT 
ACACCTGGTA CAGCAAGACA ACAGATTATC ATGTCTGCAC TGGTCAGATC ACCTGAGCAT 
CAGCCCAATG CAGTCAGCCT GCTTGCCCCA CAGAACAGCA GAAGGAAAGA ATCATCAGCT 
CCTGAAGAAT TCAGTCGCCG ACTTAAAGAA AGAATGCATC ATAATATTCC ACACAGATTT 
AATGTTGCAC TTACCATGAG AGCAGCAAAA TGTTCGGTGT GCTTGGATAC TGTTCACTTT 
GGGCGGCAGG GAGCAAAGTG CCTAGAATGT CAGGTCATGT GTCACCCAAA GTGTTCCTCC 
TGCCTCCCAG CTACATGTGG CCTCCCTGCA GAATATGCTT CACATTTCAC TGAAGCCTTT 
TGTAGAGAGA AAATGAATTC GCCTGGAATG CAGACGAAGG AAGCAAATGG TGTTGTGCGA 
CTTGAAGGCT GGATGAAAAT TCCGAGGAAT AACAAACGTG GCCAGCAAGG ATGGGACAGG 
AAATATGTGG TCTTGGAAGG GTCTAAAGTG CTGATCTATG ATACTGAATT AAAAGAAGGA 
GGACAACGGC CTTCAGAGGA GTTTGAGCTT TGTCTTCCTG ATGGAGATGT AACCGTTCAT 
GGTGCCGTTG GATCTACAGA GCTTGTAAAT ACTGCAAAAT CAGATGTGCC ATACGTCATG 
AAGATTGAGT CTCATCCACA CACAACTTGT TGGCCAGGGC GCACTATGTA CTTGTTAGCG 
CCAAGTTTTC CTGATAAGCA GCGTTGGGTT AATGCACTGG AGTCTGTGGT TGCAGGAGGA 
AGGGTGTCCA GAGAAAAAGC AGAGGCTGAT GCTAAACTGC TTGGAAACTC TTTGTTAAAA 
TTGGAGGGGG AAGACCGTCT TGATATAAAT TGCACAATAC CTCTAACTGA GCAGATTGTT 
TTGGTTGGCG CAGAGGAGGG TCTTTATGCT CTGAATGTGC TGAAGAATTC TCTCACTCAC 
ATACCAGGTA TAGGAGCAGT ATTCCAGATT CATATTGTTA AGGAGCTGGA TAAACTTCTT 
TTAATAGCAG GGGAAGAAAG GGCTCTATGC CTTGTTGATG TGAAGAAGGT GAAGCAGTCA 
TTGGCTCAGT CACATCTACC AGCACAGCCA GAAATCTCAA TGACTGTTTT TGAAGCTGTT 
AAAGGATGTC ACCTTTTTGC TGCAGGAAAG ATTGAAAATG GGTTATGCAT CTGTGCAGCC 
ATGTTAAATA AAGTTGTCAT ACTGCGCTAC AATGAAAGTC TCGGCAAGTT TTGCATAAGA 
AAGGAAATTG AAACTTCTGA GCCATGCAGT TGCATTTATT TCACTGGCTA CAGTGTCATC 
ATTGGAACAA ACAAGTTCTA TGAGATTGAG ATGAAGCAGT ACACTCTGGA GGAGTTCTTG 
GATAAAAATG ACCATTCCTT GGCTTCTGCT GTGTTTGCTT CATCTTCCAA TAGCTTCCCA 
ATAGCTATAA TGCAAGTAAA CAGCACAGGC CAGAGAGAAG AATACCTCCT TTGCTTCCAT 
GAGTTCGGTG TATTTGTTGA TTCTTATGGA AGACGTAGTC GTACTGAAGA TTTGAAATGG 
AGTCGGCTTC CTCTTGCATT TGCCTACAGG GAACCGTACT TATTTGTGAC CCATTTTAAC 
TCTTTGGAGG TGATTGAGAT CCAGGGACGT GCTGCACTAG GTGCTCCAGC TCGAGCCCAT 
CTTGATATTC CCAACCCACG TTACCTGGGA CCAGCAATCT CTTCTGGAGC CATTTATATT 
GCATCTGTTT ACCAGGATAA GCTGAGAGTA ATCTGTTGCA AGGGAAATCT AGTAAAGGAC 
ACTACAAGTG AACTTCATAG GGCTCCTTCT ACAACTCGCA GCCCAAACAA GAGAGGACCA 
CCATCTTACA ATGACCATAT AACAAAGCGA ATAGCATCCA GCCCAGTGCC AACTGAGGTC 
CAAGGTCTCG TACGGGAGCC TAGTACACCT CATAGATACC GCGAAGGACG GACAGAAATG 
CGCAGAGACA AATCTCCTGG GAGGCCTTTG GACAGGGAGA AGTCTCCAGG GAGGTTACTG 
AGCACCAGAC GGGAGAGGTC ACCTGGAAGG CTTTTTGAAG AGAGCAACAG GAGCCGTGGA 
GCTGTAAGGA CACCCTTGTC ACAAGTCAAT AAGGTTTGGG ACCAGTCTTC TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751705.1
CDS	106..6342
Translation	MLKFKFGSRSAADAISSDPITSRAARLNNFFQGKSSCQFPFQQQLNVLSREGLLDSLCALYEECCNPALMKIKHVSNFVKKYSDTVAELRELQPNVKDFEIKNVVGRGHFAEVHVVKEKVTGDIFAMKVMKKKALLAQDQVSFFEEERNILSHHNSPWIPQLHYAFQDKENLYLVMQYQPGGDLFALMNRYDEQFDENMAQFYLAELVLAIYSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKLTANKTVNAKLPVGTPDYIAPEVLMVMNGDGRSTYGPECDWWSLGIVAYEMMYGKSPFTEGTSIKTFNNIMNFQRFLKFPEEPKLSSNFLDLIQSLICGQKERLKYEDLCCHPFFSNTDWDRLRNTPPPFVPTLKSDDDTSNFDEPEKNLRILPSSCQLNSAGFSGDDLPFVGFSFIKALSLLNKSESVVSGLDSPAKVSSMEKKLFHKSKELQDTQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLNDIREQSRKLQDIKEQEFQAQLEEMKMMMHQLEEDLLAARRRSDLYETELRDSRLAAEDFKRKAVECQQKLQKAKDHGKGEVGELHTKLEKVNADQQNKIQELQEKLAKAVKASSEATELLQNIRQAKERAEKELEKLKNRDDSNDAIKKKLLDAEERRHSLENQVRRLETVERRENKLKEDIQTKSEQIQQMAEKIMELEERYREAQVGAQRLEVHLKQNEQVYEEKARNIETQMKKDLADKEALELSRIKNEEEICEKNRILSEQKAMINALESKITSLEQRLAELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKIEAQNQKLEEQLERINNQDHTDKNRLLELETRLREISLEHEEQKLELKRQLTEVQLTLQERETQISSLQSARSALESQLHQAKTELEDTTAEAEEEIQVLRSHRDEIQRKFDTLRDSCTVLTELEDQLNRISQENADLNNQNFYLSKQLSEVSGATDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWESWRSSLEDEKTQFECRLREVQRLLDTEKQSRVRSDQRTTESRQMVELAAKEHKAEILALQQALKDQKLKTESLSDKPARILNQLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENMQALFSHEKVKMEGTISQQTKLIDFLQAKMDQPSKKKKVQNGLFSRRRDEPNQTSQVPLQYNELKVALEKEKARTAELEEALQKTRIELRSAREEAAHLKVSDHPVPSTPGTARQQIIMSALVRSPEHQPNAVSLLAPQNSRRKESSAPEEFSRRLKERMHHNIPHRFNVALTMRAAKCSVCLDTVHFGRQGAKCLECQVMCHPKCSSCLPATCGLPAEYASHFTEAFCREKMNSPGMQTKEANGVVRLEGWMKIPRNNKRGQQGWDRKYVVLEGSKVLIYDTELKEGGQRPSEEFELCLPDGDVTVHGAVGSTELVNTAKSDVPYVMKIESHPHTTCWPGRTMYLLAPSFPDKQRWVNALESVVAGGRVSREKAEADAKLLGNSLLKLEGEDRLDINCTIPLTEQIVLVGAEEGLYALNVLKNSLTHIPGIGAVFQIHIVKELDKLLLIAGEERALCLVDVKKVKQSLAQSHLPAQPEISMTVFEAVKGCHLFAAGKIENGLCICAAMLNKVVILRYNESLGKFCIRKEIETSEPCSCIYFTGYSVIIGTNKFYEIEMKQYTLEEFLDKNDHSLASAVFASSSNSFPIAIMQVNSTGQREEYLLCFHEFGVFVDSYGRRSRTEDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQGRAALGAPARAHLDIPNPRYLGPAISSGAIYIASVYQDKLRVICCKGNLVKDTTSELHRAPSTTRSPNKRGPPSYNDHITKRIASSPVPTEVQGLVREPSTPHRYREGRTEMRRDKSPGRPLDREKSPGRLLSTRRERSPGRLFEESNRSRGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_031895845.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895845.1

ORF Insert Sequence:

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAT 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCCTGC CCGTATTTTG AATCAGCTAA ATGACTTGGA AAAGAAGCAT 
GCAATGCTGG AAATGAATGC TCGCAGTTTG CAACAAAAAC TGGAGACTGA AAGAGAACTA 
AAGCAACGGC TTTTAGAAGA GCAGGCTAAG TTGCAACAGC AGATGGACCT GCAAAAGAAT 
CACATTTTCC GCCTGACCCA AGGACTTCAG GAGGCCCTGG ATCGAGCTGA TCTGCTTAAA 
ACAGAAAGAA GTGATTTGGA GTATCAACTG GAAAACATGC AGGCACTCTT TTCTCATGAA 
AAAGTAAAGA TGGAAGGAAC CATTTCCCAA CAAACAAAAC TCATTGACTT CTTACAAGCA 
AAAATGGATC AGCCATCTAA GAAGAAAAAG GTACAAAATG GTTTGTTCAG TAGGCGCAGA 
GATGAACCCA ATCAAACCTC ACAGGTTCCA TTACAGTACA ATGAGCTAAA AGTAGCGCTT 
GAAAAAGAGA AGGCTAGAAC TGCAGAATTA GAAGAGGCTC TTCAAAAAAC ACGAATTGAA 
CTCCGCTCTG CAAGAGAGGA AGCTGCTCAC TTAAAGGTGT CAGATCATCC TGTTCCTTCT 
ACACCTGGTA CAGCAAGACA ACAGATTATC ATGTCTGCAC TGGTCAGATC ACCTGAGCAT 
CAGCCCAATG CAGTCAGCCT GCTTGCCCCA CAGAACAGCA GAAGGAAAGA ATCATCAGCT 
CCTGAAGAAT TCAGTCGCCG ACTTAAAGAA AGAATGCATC ATAATATTCC ACACAGATTT 
AATGTTGCAC TTACCATGAG AGCAGCAAAA TGTTCGGTGT GCTTGGATAC TGTTCACTTT 
GGGCGGCAGG GAGCAAAGTG CCTAGAATGT CAGGTCATGT GTCACCCAAA GTGTTCCTCC 
TGCCTCCCAG CTACATGTGG CCTCCCTGCA GAATATGCTT CACATTTCAC TGAAGCCTTT 
TGTAGAGAGA AAATGAATTC GCCTGGAATG CAGACGAAGG AAGCAAATGG TGTTGTGCGA 
CTTGAAGGCT GGATGAAAAT TCCGAGGAAT AACAAACGTG GCCAGCAAGG ATGGGACAGG 
AAATATGTGG TCTTGGAAGG GTCTAAAGTG CTGATCTATG ATACTGAATT AAAAGAAGGA 
GGACAACGGC CTTCAGAGGA GTTTGAGCTT TGTCTTCCTG ATGGAGATGT AACCGTTCAT 
GGTGCCGTTG GATCTACAGA GCTTGTAAAT ACTGCAAAAT CAGATGTGCC ATACGTCATG 
AAGATTGAGT CTCATCCACA CACAACTTGT TGGCCAGGGC GCACTATGTA CTTGTTAGCG 
CCAAGTTTTC CTGATAAGCA GCGTTGGGTT AATGCACTGG AGTCTGTGGT TGCAGGAGGA 
AGGGTGTCCA GAGAAAAAGC AGAGGCTGAT GCTAAACTGC TTGGAAACTC TTTGTTAAAA 
TTGGAGGGGG AAGACCGTCT TGATATAAAT TGCACAATAC CTCTAACTGA GCAGATTGTT 
TTGGTTGGCG CAGAGGAGGG TCTTTATGCT CTGAATGTGC TGAAGAATTC TCTCACTCAC 
ATACCAGGTA TAGGAGCAGT ATTCCAGATT CATATTGTTA AGGAGCTGGA TAAACTTCTT 
TTAATAGCAG GGGAAGAAAG GGCTCTATGC CTTGTTGATG TGAAGAAGGT GAAGCAGTCA 
TTGGCTCAGT CACATCTACC AGCACAGCCA GAAATCTCAA TGACTGTTTT TGAAGCTGTT 
AAAGGATGTC ACCTTTTTGC TGCAGGAAAG ATTGAAAATG GGTTATGCAT CTGTGCAGCC 
ATGTTAAATA AAGTTGTCAT ACTGCGCTAC AATGAAAGTC TCGGCAAGTT TTGCATAAGA 
AAGGAAATTG AAACTTCTGA GCCATGCAGT TGCATTTATT TCACTGGCTA CAGTGTCATC 
ATTGGAACAA ACAAGTTCTA TGAGATTGAG ATGAAGCAGT ACACTCTGGA GGAGTTCTTG 
GATAAAAATG ACCATTCCTT GGCTTCTGCT GTGTTTGCTT CATCTTCCAA TAGCTTCCCA 
ATAGCTATAA TGCAAGTAAA CAGCACAGGC CAGAGAGAAG AATACCTCCT TTGCTTCCAT 
GAGTTCGGTG TATTTGTTGA TTCTTATGGA AGACGTAGTC GTACTGAAGA TTTGAAATGG 
AGTCGGCTTC CTCTTGCATT TGCCTACAGG GAACCGTACT TATTTGTGAC CCATTTTAAC 
TCTTTGGAGG TGATTGAGAT CCAGGGACGT GCTGCACTAG GTGCTCCAGC TCGAGCCCAT 
CTTGATATTC CCAACCCACG TTACCTGGGA CCAGCAATCT CTTCTGGAGC CATTTATATT 
GCATCTGTTT ACCAGGATAA GCTGAGAGTA ATCTGTTGCA AGGGAAATCT AGTAAAGGAC 
ACTACAAGTG AACTTCATAG GGCTCCTTCT ACAACTCGCA GCCCAAACAA GAGAGGACCA 
CCATCTTACA ATGACCATAT AACAAAGCGA ATAGCATCCA GCCCAGTGCC AACTGAGGTC 
CAAGGTCTCG TACGGGAGCC TAGTACACCT CATAGATACC GCGAAGGACG GACAGAAATG 
CGCAGAGACA AATCTCCTGG GAGGCCTTTG GACAGGGAGA AGTCTCCAGG GAGGTTACTG 
AGCACCAGAC GGGAGAGGTC ACCTGGAAGG CTTTTTGAAG AGAGCAACAG GAGCCGTGGA 
GCTGTAAGGA CACCCTTGTC ACAAGTCAAT AAGGTTTGGG ACCAGTCTTC TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cit ( NM_001127129.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001120601.1 Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), mRNA.

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CloneID	OXa02127
Clone ID Related Accession (Same CDS sequence)	NM_001127129.1
Accession Version	NM_001127129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Xenopus tropicalis(tropical clawed frog)
Product	citron Rho-interacting kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161636.1. ##Evidence-Data-START## Transcript exon combination :: BC161636.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACA CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTCGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAA 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCTAAA TGACTTGGAA AAGAAGCATG CAATGCTGGA AATGAATGCT 
CGCAGTTTGC AACAAAAACT GGAGACTGAA AGAGAACTAA AGCAACGGCT TTTAGAAGAG 
CAGGCTAAGT TGCAACAGCA GATGGACCTG CAAAAGAATC ACATTTTCCG CCTGACCCAA 
GGACTTCAGG AGGCCCTGGA TCGAGCTGAT CTGCTTAAAA CAGAAAGAAG TGATTTGGAG 
TATCAACTGG AAAACATGCA GGCACTCTTT TCTCATGAAA AAGTAAAGAT GGAAGGAACC 
ATTTCCCAAC AAACAAAACT CATTGACTTC TTACAAGCAA AAATGGATCA GCCATCTAAG 
AAGAAAAAGG GTTTGTTCAG TAGGCGCAGA GATGAACCCA ATCAAACCTC ACAGGTTCCA 
TTACAGTACA ATGAGCTAAA AGTAGCGCTT GAAAAAGAGA AGGCTAGAAC TGCAGAATTA 
GAAGAGGCTC TTCAAAAAAC ACGAATTGAA CTCCGCTCTG CAAGAGAGGA AGCTGCTCAC 
TTAAAGGTGT CAGATCATCC TGTTCCTTCT ACACCTGGTA CAGCAAGACA ACAGATTATC 
ATGTCTGCAC TGGTCAGATC ACCTGAGCAT CAGCCCAATG CAGTCAGCCT GCTTGCCCCA 
CAGAACAGCA GAAGGAAAGA ATCATCAGCT CCTGAAGAAT TCAGTCGCCG ACTTAAAGAA 
AGAATGCATC ATAATATTCC ACACAGATTT AATGTTGCAC TTACCATGAG AGCAGCAAAA 
TGTTCGGTGT GCTTGGATAC TGTTCACTTT GGGCGGCAGG GAGCAAAGTG CCTAGAATGT 
CAGGTCATGT GTCACCCAAA GTGTTCCTCC TGCCTCCCAG CTACATGTGG CCTCCCTGCA 
GAATATGCTT CACATTTCAC TGAAGCCTTT TGTAGAGAGA AAATGAATTC GCCTGGAATG 
CAGACGAAGG AAGCAAATGG TGTTGTGCGA CTTGAAGGCT GGATGAAAAT TCCGAGGAAT 
AACAAACGTG GCCAGCAAGG ATGGGACAGG AAATATGTGG TCTTGGAAGG GTCTAAAGTG 
CTGATCTATG ATACTGAATT AAAAGAAGGA GGACAACGGC CTTCAGAGGA GTTTGAGCTT 
TGTCTTCCTG ATGGAGATGT AACCGTTCAT GGTGCCGTTG GATCTACAGA GCTTGTAAAT 
ACTGCAAAAT CAGATGTGCC ATACGTCATG AAGATTGAGT CTCATCCACA CACAACTTGT 
TGGCCAGGGC GCACTATGTA CTTGTTAGCG CCAAGTTTTC CTGATAAGCA GCGTTGGGTT 
AATGCACTGG AGTCTGTGGT TGCAGGAGGA AGGGTGTCCA GAGAAAAAGC AGAGGCTGAT 
GCTAAACTGC TTGGAAACTC TTTGTTAAAA TTGGAGGGGG AAGACCGTCT TGATATAAAT 
TGCACAATAC CTCTAACTGA GCAGATTGTT TTGGTTGGCG CAGAGGAGGG TCTTTATGCT 
CTGAATGTGC TGAAGAATTC TCTCACTCAC ATACCAGGTA TAGGAGCAGT ATTCCAGATT 
CATATTGTTA AGGAGCTGGA TAAACTTCTT TTAATAGCAG GGGAAGAAAG GGCTCTATGC 
CTTGTTGATG TGAAGAAGGT GAAGCAGTCA TTGGCTCAGT CACATCTACC AGCACAGCCA 
GAAATCTCAA TGACTGTTTT TGAAGCTGTT AAAGGATGTC ACCTTTTTGC TGCAGGAAAG 
ATTGAAAATG GGTTATGCAT CTGTGCAGCC ATGTTAAATA AAGTTGTCAT ACTGCGCTAC 
AATGAAAGTC TCGGCAAGTT TTGCATAAGA AAGGAAATTG AAACTTCTGA GCCATGCAGT 
TGCATTTATT TCACTGGCTA CAGTGTCATC ATTGGAACAA ACAAGTTCTA TGAGATTGAG 
ATGAAGCAGT ACACTCTGGA GGAGTTCTTG GATAAAAATG ACCATTCCTT GGCTTCTGCT 
GTGTTTGCTT CATCTTCCAA TAGCTTCCCA ATAGCTATAA TGCAAGTAAA CAGCACAGGC 
CAGAGAGAAG AATACCTCCT TTGCTTCCAT GAGTTCGGTG TATTTGTTGA TTCTTATGGA 
AGACGTAGTC GTACTGAAGA TTTGAAATGG AGTCGGCTTC CTCTTGCATT TGCCTACAGG 
GAACCGTACT TATTTGTGAC CCATTTTAAC TCTTTGGAGG TGATTGAGAT CCAGGGACGT 
GCTGCACTAG GTGCTCCAGC TCGAGCCCAT CTTGATATTC CCAACCCACG TTACCTGGGA 
CCAGCAATCT CTTCTGGAGC CATTTATATT GCATCTGTTT ACCAGGATAA GCTGAGAGTA 
ATCTGTTGCA AGGGAAATCT AGTAAAGGAC ACTACAAGTG AACTTCATAG GGCTCCTTCT 
ACAACTCGCA GCCCAAACAA GAGAGGACCA CCATCTTACA ATGACCATAT AACAAAGCGA 
ATAGCATCCA GCCCAGTGCC AACTGAGGTC CAAGGTCTCG TACGGGAGCC TAGTACACCT 
CATAGATACC GCGAAGGACG GACAGAAATG CGCAGAGACA AATCTCCTGG GAGGCCTTTG 
GACAGGGAGA AGTCTCCAGG GAGGTTACTG AGCACCAGAC GGGAGAGGTC ACCTGGAAGG 
CTTTTTGAAG AGAGCAACAG GAGCCGTGGA GCTGTAAGGA CACCCTTGTC ACAAGTCAAT 
AAGGTTTGGG ACCAGTCTTC TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120601.1
CDS	23..6229
Translation	MLKFKFGSRSAADAISSDPITSRAARLNNFFQGKSSCQFPFQQQLNVLSREGLLDSLCALYEECCNPALMKIKHVSNFVKKYSDTVAELRELQPNVKDFEIKNVVGHGHFAEVHVVKEKVTGDIFAMKVMKKKALLAQDQVSFFEEERNILSHHNSPWIPQLHYAFQDKENLYLVMQYQPGGDLFALMNRYDEQFDENMAQFYLAELVLAIYSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKLTANKTVNAKLPVGTPDYIAPEVLMVMNGDGRSTYGPECDWWSLGIVAYEMMYGKSPFTEGTSIKTFNNIMNFQRFLKFPEEPKLSSNFLDLIQSLICGQKERLKYEDLCCHPFFSNTDWDRLRNTPPPFVPTLKSDDDTSNFDEPEKNLRILPSSCQLNSAGFSGDDLPFVGFSFIKALSLLNKSESVVSGLDSPAKVSSMEKKLFHKSKELQDTQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLNDIREQSRKLQDIKEQEFQAQLEEMKMMMHQLEEDLLAARRRSDLYETELRDSRLAAEEFKRKAVECQQKLQKAKDHGKGEVGELHTKLEKVNADQQNKIQELQEKLAKAVKASSEATELLQNIRQAKERAEKELEKLKNRDDSNDAIKKKLLDAEERRHSLENQVRRLETVERRENKLKEDIQTKSEQIQQMAEKIMELEERYREAQVGAQRLEVHLKQNEQVYEEKARNIETQMKKDLADKEALELSRIKNEEEICEKNRILSEQKAMINALESKITSLEQRLAELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKIEAQNQKLEEQLERINNQDHTDKNRLLELETRLREISLEHEEQKLELKRQLTEVQLTLQERETQISSLQSARSALESQLHQAKTELEDTTAEAEEEIQVLRSHRDEIQRKFDTLRDSCTVLTELEDQLNRISQENADLNNQNFYLSKQLSEVSGATDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWESWRSSLEDEKTQFECRLREVQRLLDTEKQSRVRSDQRTTESRQMVELAAKEHKAEILALQQALKDQKLKTESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENMQALFSHEKVKMEGTISQQTKLIDFLQAKMDQPSKKKKGLFSRRRDEPNQTSQVPLQYNELKVALEKEKARTAELEEALQKTRIELRSAREEAAHLKVSDHPVPSTPGTARQQIIMSALVRSPEHQPNAVSLLAPQNSRRKESSAPEEFSRRLKERMHHNIPHRFNVALTMRAAKCSVCLDTVHFGRQGAKCLECQVMCHPKCSSCLPATCGLPAEYASHFTEAFCREKMNSPGMQTKEANGVVRLEGWMKIPRNNKRGQQGWDRKYVVLEGSKVLIYDTELKEGGQRPSEEFELCLPDGDVTVHGAVGSTELVNTAKSDVPYVMKIESHPHTTCWPGRTMYLLAPSFPDKQRWVNALESVVAGGRVSREKAEADAKLLGNSLLKLEGEDRLDINCTIPLTEQIVLVGAEEGLYALNVLKNSLTHIPGIGAVFQIHIVKELDKLLLIAGEERALCLVDVKKVKQSLAQSHLPAQPEISMTVFEAVKGCHLFAAGKIENGLCICAAMLNKVVILRYNESLGKFCIRKEIETSEPCSCIYFTGYSVIIGTNKFYEIEMKQYTLEEFLDKNDHSLASAVFASSSNSFPIAIMQVNSTGQREEYLLCFHEFGVFVDSYGRRSRTEDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQGRAALGAPARAHLDIPNPRYLGPAISSGAIYIASVYQDKLRVICCKGNLVKDTTSELHRAPSTTRSPNKRGPPSYNDHITKRIASSPVPTEVQGLVREPSTPHRYREGRTEMRRDKSPGRPLDREKSPGRLLSTRRERSPGRLFEESNRSRGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	NM_001127129.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127129.1

ORF Insert Sequence:

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACA CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTCGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAA 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCTAAA TGACTTGGAA AAGAAGCATG CAATGCTGGA AATGAATGCT 
CGCAGTTTGC AACAAAAACT GGAGACTGAA AGAGAACTAA AGCAACGGCT TTTAGAAGAG 
CAGGCTAAGT TGCAACAGCA GATGGACCTG CAAAAGAATC ACATTTTCCG CCTGACCCAA 
GGACTTCAGG AGGCCCTGGA TCGAGCTGAT CTGCTTAAAA CAGAAAGAAG TGATTTGGAG 
TATCAACTGG AAAACATGCA GGCACTCTTT TCTCATGAAA AAGTAAAGAT GGAAGGAACC 
ATTTCCCAAC AAACAAAACT CATTGACTTC TTACAAGCAA AAATGGATCA GCCATCTAAG 
AAGAAAAAGG GTTTGTTCAG TAGGCGCAGA GATGAACCCA ATCAAACCTC ACAGGTTCCA 
TTACAGTACA ATGAGCTAAA AGTAGCGCTT GAAAAAGAGA AGGCTAGAAC TGCAGAATTA 
GAAGAGGCTC TTCAAAAAAC ACGAATTGAA CTCCGCTCTG CAAGAGAGGA AGCTGCTCAC 
TTAAAGGTGT CAGATCATCC TGTTCCTTCT ACACCTGGTA CAGCAAGACA ACAGATTATC 
ATGTCTGCAC TGGTCAGATC ACCTGAGCAT CAGCCCAATG CAGTCAGCCT GCTTGCCCCA 
CAGAACAGCA GAAGGAAAGA ATCATCAGCT CCTGAAGAAT TCAGTCGCCG ACTTAAAGAA 
AGAATGCATC ATAATATTCC ACACAGATTT AATGTTGCAC TTACCATGAG AGCAGCAAAA 
TGTTCGGTGT GCTTGGATAC TGTTCACTTT GGGCGGCAGG GAGCAAAGTG CCTAGAATGT 
CAGGTCATGT GTCACCCAAA GTGTTCCTCC TGCCTCCCAG CTACATGTGG CCTCCCTGCA 
GAATATGCTT CACATTTCAC TGAAGCCTTT TGTAGAGAGA AAATGAATTC GCCTGGAATG 
CAGACGAAGG AAGCAAATGG TGTTGTGCGA CTTGAAGGCT GGATGAAAAT TCCGAGGAAT 
AACAAACGTG GCCAGCAAGG ATGGGACAGG AAATATGTGG TCTTGGAAGG GTCTAAAGTG 
CTGATCTATG ATACTGAATT AAAAGAAGGA GGACAACGGC CTTCAGAGGA GTTTGAGCTT 
TGTCTTCCTG ATGGAGATGT AACCGTTCAT GGTGCCGTTG GATCTACAGA GCTTGTAAAT 
ACTGCAAAAT CAGATGTGCC ATACGTCATG AAGATTGAGT CTCATCCACA CACAACTTGT 
TGGCCAGGGC GCACTATGTA CTTGTTAGCG CCAAGTTTTC CTGATAAGCA GCGTTGGGTT 
AATGCACTGG AGTCTGTGGT TGCAGGAGGA AGGGTGTCCA GAGAAAAAGC AGAGGCTGAT 
GCTAAACTGC TTGGAAACTC TTTGTTAAAA TTGGAGGGGG AAGACCGTCT TGATATAAAT 
TGCACAATAC CTCTAACTGA GCAGATTGTT TTGGTTGGCG CAGAGGAGGG TCTTTATGCT 
CTGAATGTGC TGAAGAATTC TCTCACTCAC ATACCAGGTA TAGGAGCAGT ATTCCAGATT 
CATATTGTTA AGGAGCTGGA TAAACTTCTT TTAATAGCAG GGGAAGAAAG GGCTCTATGC 
CTTGTTGATG TGAAGAAGGT GAAGCAGTCA TTGGCTCAGT CACATCTACC AGCACAGCCA 
GAAATCTCAA TGACTGTTTT TGAAGCTGTT AAAGGATGTC ACCTTTTTGC TGCAGGAAAG 
ATTGAAAATG GGTTATGCAT CTGTGCAGCC ATGTTAAATA AAGTTGTCAT ACTGCGCTAC 
AATGAAAGTC TCGGCAAGTT TTGCATAAGA AAGGAAATTG AAACTTCTGA GCCATGCAGT 
TGCATTTATT TCACTGGCTA CAGTGTCATC ATTGGAACAA ACAAGTTCTA TGAGATTGAG 
ATGAAGCAGT ACACTCTGGA GGAGTTCTTG GATAAAAATG ACCATTCCTT GGCTTCTGCT 
GTGTTTGCTT CATCTTCCAA TAGCTTCCCA ATAGCTATAA TGCAAGTAAA CAGCACAGGC 
CAGAGAGAAG AATACCTCCT TTGCTTCCAT GAGTTCGGTG TATTTGTTGA TTCTTATGGA 
AGACGTAGTC GTACTGAAGA TTTGAAATGG AGTCGGCTTC CTCTTGCATT TGCCTACAGG 
GAACCGTACT TATTTGTGAC CCATTTTAAC TCTTTGGAGG TGATTGAGAT CCAGGGACGT 
GCTGCACTAG GTGCTCCAGC TCGAGCCCAT CTTGATATTC CCAACCCACG TTACCTGGGA 
CCAGCAATCT CTTCTGGAGC CATTTATATT GCATCTGTTT ACCAGGATAA GCTGAGAGTA 
ATCTGTTGCA AGGGAAATCT AGTAAAGGAC ACTACAAGTG AACTTCATAG GGCTCCTTCT 
ACAACTCGCA GCCCAAACAA GAGAGGACCA CCATCTTACA ATGACCATAT AACAAAGCGA 
ATAGCATCCA GCCCAGTGCC AACTGAGGTC CAAGGTCTCG TACGGGAGCC TAGTACACCT 
CATAGATACC GCGAAGGACG GACAGAAATG CGCAGAGACA AATCTCCTGG GAGGCCTTTG 
GACAGGGAGA AGTCTCCAGG GAGGTTACTG AGCACCAGAC GGGAGAGGTC ACCTGGAAGG 
CTTTTTGAAG AGAGCAACAG GAGCCGTGGA GCTGTAAGGA CACCCTTGTC ACAAGTCAAT 
AAGGTTTGGG ACCAGTCTTC TGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cit ( XM_012956618.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812072.1 Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X2, mRNA.

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CloneID	OXa32034
Clone ID Related Accession (Same CDS sequence)	XM_012956618.2
Accession Version	XM_012956618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	citron Rho-interacting kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956618.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAA 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGAAAGC CCAAGAGGAG ATGATTTCAG AACTCAGGCA GCAAAAGTTT 
TACCTGGAAA CACAGGCAGG AAAAATAGAG GCTCAGAATC AGAAACTGGA GGAACAGCTA 
GAGAGAATAA ATAATCAGGA CCACACTGAC AAAAACCGCC TTCTAGAACT GGAGACAAGG 
CTGCGAGAGA TTAGTTTGGA GCATGAAGAA CAAAAGCTGG AGCTAAAGAG ACAGTTAACA 
GAGGTGCAGC TCACATTACA GGAAAGAGAA ACCCAGATCT CCAGTTTGCA ATCTGCACGT 
TCTGCATTAG AGAGTCAGCT ACATCAGGCA AAAACAGAGC TGGAAGATAC AACTGCAGAG 
GCTGAGGAAG AGATTCAAGT TTTAAGGTCT CACAGAGATG AGATCCAGCG CAAATTTGAT 
ACTTTGCGTG ACAGTTGTAC GGTCCTTACA GAGCTAGAGG ATCAGCTTAA TCGTATCAGT 
CAAGAAAATG CAGATTTGAA CAATCAAAAC TTTTATTTGT CAAAGCAACT TAGTGAGGTT 
TCAGGAGCCA CTGATGAAAT TGTGCAACTG CGAAGTGAAG TGGATCATCT CAGAAGAGAA 
ATCACAGAAA GAGAGATGCA ACTCACAAGT CAAAAGCAGA CCATGGAGGC CCTGAAAACT 
ACATGTACCA TGCTAGAGGA ACAAGTGCTG GATCTTGAAG CATTGAATGA TGAGTTGCTA 
GAAAAGGAAC GTCAGTGGGA GAGCTGGCGT AGTTCCCTAG AAGATGAGAA AACTCAGTTT 
GAATGTAGAC TTCGAGAGGT ACAGAGATTG CTGGATACCG AAAAACAGAG CAGGGTACGA 
TCTGACCAAC GAACTACAGA ATCACGCCAG ATGGTGGAAT TAGCTGCCAA GGAACACAAG 
GCTGAAATCT TAGCTTTACA GCAAGCACTG AAAGATCAGA AGCTCAAGAC AGAAAGTCTT 
TCAGATAAGC TAAATGACTT GGAAAAGAAG CATGCAATGC TGGAAATGAA TGCTCGCAGT 
TTGCAACAAA AACTGGAGAC TGAAAGAGAA CTAAAGCAAC GGCTTTTAGA AGAGCAGGCT 
AAGTTGCAAC AGCAGATGGA CCTGCAAAAG AATCACATTT TCCGCCTGAC CCAAGGACTT 
CAGGAGGCCC TGGATCGAGC TGATCTGCTT AAAACAGAAA GAAGTGATTT GGAGTATCAA 
CTGGAAAACA TGCAGGCACT CTTTTCTCAT GAAAAAGTAA AGATGGAAGG AACCATTTCC 
CAACAAACAA AACTCATTGA CTTCTTACAA GCAAAAATGG ATCAGCCATC TAAGAAGAAA 
AAGGTACAAA ATGGTTTGTT CAGTAGGCGC AGAGATGAAC CCAATCAAAC CTCACAGGTT 
CCATTACAGT ACAATGAGCT AAAAGTAGCG CTTGAAAAAG AGAAGGCTAG AACTGCAGAA 
TTAGAAGAGG CTCTTCAAAA AACACGAATT GAACTCCGCT CTGCAAGAGA GGAAGCTGCT 
CACTTAAAGG TGTCAGATCA TCCTGTTCCT TCTACACCTG GTACAGCAAG ACAACAGATT 
ATCATGTCTG CACTGGTCAG ATCACCTGAG CATCAGCCCA ATGCAGTCAG CCTGCTTGCC 
CCACAGAACA GCAGAAGGAA AGAATCATCA GCTCCTGAAG AATTCAGTCG CCGACTTAAA 
GAAAGAATGC ATCATAATAT TCCACACAGA TTTAATGTTG CACTTACCAT GAGAGCAGCA 
AAATGTTCGG TGTGCTTGGA TACTGTTCAC TTTGGGCGGC AGGGAGCAAA GTGCCTAGAA 
TGTCAGGTCA TGTGTCACCC AAAGTGTTCC TCCTGCCTCC CAGCTACATG TGGCCTCCCT 
GCAGAATATG CTTCACATTT CACTGAAGCC TTTTGTAGAG AGAAAATGAA TTCGCCTGGA 
ATGCAGACGA AGGAAGCAAA TGGTGTTGTG CGACTTGAAG GCTGGATGAA AATTCCGAGG 
AATAACAAAC GTGGCCAGCA AGGATGGGAC AGGAAATATG TGGTCTTGGA AGGGTCTAAA 
GTGCTGATCT ATGATACTGA ATTAAAAGAA GGAGGACAAC GGCCTTCAGA GGAGTTTGAG 
CTTTGTCTTC CTGATGGAGA TGTAACCGTT CATGGTGCCG TTGGATCTAC AGAGCTTGTA 
AATACTGCAA AATCAGATGT GCCATACGTC ATGAAGATTG AGTCTCATCC ACACACAACT 
TGTTGGCCAG GGCGCACTAT GTACTTGTTA GCGCCAAGTT TTCCTGATAA GCAGCGTTGG 
GTTAATGCAC TGGAGTCTGT GGTTGCAGGA GGAAGGGTGT CCAGAGAAAA AGCAGAGGCT 
GATGCTAAAC TGCTTGGAAA CTCTTTGTTA AAATTGGAGG GGGAAGACCG TCTTGATATA 
AATTGCACAA TACCTCTAAC TGAGCAGATT GTTTTGGTTG GCGCAGAGGA GGGTCTTTAT 
GCTCTGAATG TGCTGAAGAA TTCTCTCACT CACATACCAG GTATAGGAGC AGTATTCCAG 
ATTCATATTG TTAAGGAGCT GGATAAACTT CTTTTAATAG CAGGGGAAGA AAGGGCTCTA 
TGCCTTGTTG ATGTGAAGAA GGTGAAGCAG TCATTGGCTC AGTCACATCT ACCAGCACAG 
CCAGAAATCT CAATGACTGT TTTTGAAGCT GTTAAAGGAT GTCACCTTTT TGCTGCAGGA 
AAGATTGAAA ATGGGTTATG CATCTGTGCA GCCATGTTAA ATAAAGTTGT CATACTGCGC 
TACAATGAAA GTCTCGGCAA GTTTTGCATA AGAAAGGAAA TTGAAACTTC TGAGCCATGC 
AGTTGCATTT ATTTCACTGG CTACAGTGTC ATCATTGGAA CAAACAAGTT CTATGAGATT 
GAGATGAAGC AGTACACTCT GGAGGAGTTC TTGGATAAAA ATGACCATTC CTTGGCTTCT 
GCTGTGTTTG CTTCATCTTC CAATAGCTTC CCAATAGCTA TAATGCAAGT AAACAGCACA 
GGCCAGAGAG AAGAATACCT CCTTTGCTTC CATGAGTTCG GTGTATTTGT TGATTCTTAT 
GGAAGACGTA GTCGTACTGA AGATTTGAAA TGGAGTCGGC TTCCTCTTGC ATTTGCCTAC 
AGGGAACCGT ACTTATTTGT GACCCATTTT AACTCTTTGG AGGTGATTGA GATCCAGGGA 
CGTGCTGCAC TAGGTGCTCC AGCTCGAGCC CATCTTGATA TTCCCAACCC ACGTTACCTG 
GGACCAGCAA TCTCTTCTGG AGCCATTTAT ATTGCATCTG TTTACCAGGA TAAGCTGAGA 
GTAATCTGTT GCAAGGGAAA TCTAGTAAAG GACACTACAA GTGAACTTCA TAGGGCTCCT 
TCTACAACTC GCAGCCCAAA CAAGAGAGGA CCACCATCTT ACAATGACCA TATAACAAAG 
CGAATAGCAT CCAGCCCAGT GCCAACTGAG GTCCAAGGTC TCGTACGGGA GCCTAGTACA 
CCTCATAGAT ACCGCGAAGG ACGGACAGAA ATGCGCAGAG ACAAATCTCC TGGGAGGCCT 
TTGGACAGGG AGAAGTCTCC AGGGAGGTTA CTGAGCACCA GACGGGAGAG GTCACCTGGA 
AGGCTTTTTG AAGAGAGCAA CAGGAGCCGT GGAGCTGTAA GGACACCCTT GTCACAAGTC 
AATAAGGTTT GGGACCAGTC TTCTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812072.1
CDS	876..7025
Translation	MLKFKFGSRSAADAISSDPITSRAARLNNFFQGKSSCQFPFQQQLNVLSREGLLDSLCALYEECCNPALMKIKHVSNFVKKYSDTVAELRELQPNVKDFEIKNVVGRGHFAEVHVVKEKVTGDIFAMKVMKKKALLAQDQVSFFEEERNILSHHNSPWIPQLHYAFQDKENLYLVMQYQPGGDLFALMNRYDEQFDENMAQFYLAELVLAIYSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKLTANKTVNAKLPVGTPDYIAPEVLMVMNGDGRSTYGPECDWWSLGIVAYEMMYGKSPFTEGTSIKTFNNIMNFQRFLKFPEEPKLSSNFLDLIQSLICGQKERLKYEDLCCHPFFSNTDWDRLRNTPPPFVPTLKSDDDTSNFDEPEKNLRILPSSCQLNSAGFSGDDLPFVGFSFIKALSLLNKSESVVSGLDSPAKVSSMEKKLFHKSKELQDTQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLNDIREQSRKLQDIKEQEFQAQLEEMKMMMHQLEEDLLAARRRSDLYETELRDSRLAAEEFKRKAVECQQKLQKAKDHGKGEVGELHTKLEKVNADQQNKIQELQEKLAKAVKASSEATELLQNIRQAKERAEKELEKLKNRDDSNDAIKKKLLDAEERRHSLENQVRRLETVERRENKLKEDIQTKSEQIQQMAEKIMELEERYREAQVGAQRLEVHLKQNEQVYEEKARNIETQMKKDLADKEALELSRIKNEEEICEKNRILSEQKAMINALESKITSLEQRKAQEEMISELRQQKFYLETQAGKIEAQNQKLEEQLERINNQDHTDKNRLLELETRLREISLEHEEQKLELKRQLTEVQLTLQERETQISSLQSARSALESQLHQAKTELEDTTAEAEEEIQVLRSHRDEIQRKFDTLRDSCTVLTELEDQLNRISQENADLNNQNFYLSKQLSEVSGATDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWESWRSSLEDEKTQFECRLREVQRLLDTEKQSRVRSDQRTTESRQMVELAAKEHKAEILALQQALKDQKLKTESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENMQALFSHEKVKMEGTISQQTKLIDFLQAKMDQPSKKKKVQNGLFSRRRDEPNQTSQVPLQYNELKVALEKEKARTAELEEALQKTRIELRSAREEAAHLKVSDHPVPSTPGTARQQIIMSALVRSPEHQPNAVSLLAPQNSRRKESSAPEEFSRRLKERMHHNIPHRFNVALTMRAAKCSVCLDTVHFGRQGAKCLECQVMCHPKCSSCLPATCGLPAEYASHFTEAFCREKMNSPGMQTKEANGVVRLEGWMKIPRNNKRGQQGWDRKYVVLEGSKVLIYDTELKEGGQRPSEEFELCLPDGDVTVHGAVGSTELVNTAKSDVPYVMKIESHPHTTCWPGRTMYLLAPSFPDKQRWVNALESVVAGGRVSREKAEADAKLLGNSLLKLEGEDRLDINCTIPLTEQIVLVGAEEGLYALNVLKNSLTHIPGIGAVFQIHIVKELDKLLLIAGEERALCLVDVKKVKQSLAQSHLPAQPEISMTVFEAVKGCHLFAAGKIENGLCICAAMLNKVVILRYNESLGKFCIRKEIETSEPCSCIYFTGYSVIIGTNKFYEIEMKQYTLEEFLDKNDHSLASAVFASSSNSFPIAIMQVNSTGQREEYLLCFHEFGVFVDSYGRRSRTEDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQGRAALGAPARAHLDIPNPRYLGPAISSGAIYIASVYQDKLRVICCKGNLVKDTTSELHRAPSTTRSPNKRGPPSYNDHITKRIASSPVPTEVQGLVREPSTPHRYREGRTEMRRDKSPGRPLDREKSPGRLLSTRRERSPGRLFEESNRSRGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_012956618.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956618.2

ORF Insert Sequence:

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAA 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGAAAGC CCAAGAGGAG ATGATTTCAG AACTCAGGCA GCAAAAGTTT 
TACCTGGAAA CACAGGCAGG AAAAATAGAG GCTCAGAATC AGAAACTGGA GGAACAGCTA 
GAGAGAATAA ATAATCAGGA CCACACTGAC AAAAACCGCC TTCTAGAACT GGAGACAAGG 
CTGCGAGAGA TTAGTTTGGA GCATGAAGAA CAAAAGCTGG AGCTAAAGAG ACAGTTAACA 
GAGGTGCAGC TCACATTACA GGAAAGAGAA ACCCAGATCT CCAGTTTGCA ATCTGCACGT 
TCTGCATTAG AGAGTCAGCT ACATCAGGCA AAAACAGAGC TGGAAGATAC AACTGCAGAG 
GCTGAGGAAG AGATTCAAGT TTTAAGGTCT CACAGAGATG AGATCCAGCG CAAATTTGAT 
ACTTTGCGTG ACAGTTGTAC GGTCCTTACA GAGCTAGAGG ATCAGCTTAA TCGTATCAGT 
CAAGAAAATG CAGATTTGAA CAATCAAAAC TTTTATTTGT CAAAGCAACT TAGTGAGGTT 
TCAGGAGCCA CTGATGAAAT TGTGCAACTG CGAAGTGAAG TGGATCATCT CAGAAGAGAA 
ATCACAGAAA GAGAGATGCA ACTCACAAGT CAAAAGCAGA CCATGGAGGC CCTGAAAACT 
ACATGTACCA TGCTAGAGGA ACAAGTGCTG GATCTTGAAG CATTGAATGA TGAGTTGCTA 
GAAAAGGAAC GTCAGTGGGA GAGCTGGCGT AGTTCCCTAG AAGATGAGAA AACTCAGTTT 
GAATGTAGAC TTCGAGAGGT ACAGAGATTG CTGGATACCG AAAAACAGAG CAGGGTACGA 
TCTGACCAAC GAACTACAGA ATCACGCCAG ATGGTGGAAT TAGCTGCCAA GGAACACAAG 
GCTGAAATCT TAGCTTTACA GCAAGCACTG AAAGATCAGA AGCTCAAGAC AGAAAGTCTT 
TCAGATAAGC TAAATGACTT GGAAAAGAAG CATGCAATGC TGGAAATGAA TGCTCGCAGT 
TTGCAACAAA AACTGGAGAC TGAAAGAGAA CTAAAGCAAC GGCTTTTAGA AGAGCAGGCT 
AAGTTGCAAC AGCAGATGGA CCTGCAAAAG AATCACATTT TCCGCCTGAC CCAAGGACTT 
CAGGAGGCCC TGGATCGAGC TGATCTGCTT AAAACAGAAA GAAGTGATTT GGAGTATCAA 
CTGGAAAACA TGCAGGCACT CTTTTCTCAT GAAAAAGTAA AGATGGAAGG AACCATTTCC 
CAACAAACAA AACTCATTGA CTTCTTACAA GCAAAAATGG ATCAGCCATC TAAGAAGAAA 
AAGGTACAAA ATGGTTTGTT CAGTAGGCGC AGAGATGAAC CCAATCAAAC CTCACAGGTT 
CCATTACAGT ACAATGAGCT AAAAGTAGCG CTTGAAAAAG AGAAGGCTAG AACTGCAGAA 
TTAGAAGAGG CTCTTCAAAA AACACGAATT GAACTCCGCT CTGCAAGAGA GGAAGCTGCT 
CACTTAAAGG TGTCAGATCA TCCTGTTCCT TCTACACCTG GTACAGCAAG ACAACAGATT 
ATCATGTCTG CACTGGTCAG ATCACCTGAG CATCAGCCCA ATGCAGTCAG CCTGCTTGCC 
CCACAGAACA GCAGAAGGAA AGAATCATCA GCTCCTGAAG AATTCAGTCG CCGACTTAAA 
GAAAGAATGC ATCATAATAT TCCACACAGA TTTAATGTTG CACTTACCAT GAGAGCAGCA 
AAATGTTCGG TGTGCTTGGA TACTGTTCAC TTTGGGCGGC AGGGAGCAAA GTGCCTAGAA 
TGTCAGGTCA TGTGTCACCC AAAGTGTTCC TCCTGCCTCC CAGCTACATG TGGCCTCCCT 
GCAGAATATG CTTCACATTT CACTGAAGCC TTTTGTAGAG AGAAAATGAA TTCGCCTGGA 
ATGCAGACGA AGGAAGCAAA TGGTGTTGTG CGACTTGAAG GCTGGATGAA AATTCCGAGG 
AATAACAAAC GTGGCCAGCA AGGATGGGAC AGGAAATATG TGGTCTTGGA AGGGTCTAAA 
GTGCTGATCT ATGATACTGA ATTAAAAGAA GGAGGACAAC GGCCTTCAGA GGAGTTTGAG 
CTTTGTCTTC CTGATGGAGA TGTAACCGTT CATGGTGCCG TTGGATCTAC AGAGCTTGTA 
AATACTGCAA AATCAGATGT GCCATACGTC ATGAAGATTG AGTCTCATCC ACACACAACT 
TGTTGGCCAG GGCGCACTAT GTACTTGTTA GCGCCAAGTT TTCCTGATAA GCAGCGTTGG 
GTTAATGCAC TGGAGTCTGT GGTTGCAGGA GGAAGGGTGT CCAGAGAAAA AGCAGAGGCT 
GATGCTAAAC TGCTTGGAAA CTCTTTGTTA AAATTGGAGG GGGAAGACCG TCTTGATATA 
AATTGCACAA TACCTCTAAC TGAGCAGATT GTTTTGGTTG GCGCAGAGGA GGGTCTTTAT 
GCTCTGAATG TGCTGAAGAA TTCTCTCACT CACATACCAG GTATAGGAGC AGTATTCCAG 
ATTCATATTG TTAAGGAGCT GGATAAACTT CTTTTAATAG CAGGGGAAGA AAGGGCTCTA 
TGCCTTGTTG ATGTGAAGAA GGTGAAGCAG TCATTGGCTC AGTCACATCT ACCAGCACAG 
CCAGAAATCT CAATGACTGT TTTTGAAGCT GTTAAAGGAT GTCACCTTTT TGCTGCAGGA 
AAGATTGAAA ATGGGTTATG CATCTGTGCA GCCATGTTAA ATAAAGTTGT CATACTGCGC 
TACAATGAAA GTCTCGGCAA GTTTTGCATA AGAAAGGAAA TTGAAACTTC TGAGCCATGC 
AGTTGCATTT ATTTCACTGG CTACAGTGTC ATCATTGGAA CAAACAAGTT CTATGAGATT 
GAGATGAAGC AGTACACTCT GGAGGAGTTC TTGGATAAAA ATGACCATTC CTTGGCTTCT 
GCTGTGTTTG CTTCATCTTC CAATAGCTTC CCAATAGCTA TAATGCAAGT AAACAGCACA 
GGCCAGAGAG AAGAATACCT CCTTTGCTTC CATGAGTTCG GTGTATTTGT TGATTCTTAT 
GGAAGACGTA GTCGTACTGA AGATTTGAAA TGGAGTCGGC TTCCTCTTGC ATTTGCCTAC 
AGGGAACCGT ACTTATTTGT GACCCATTTT AACTCTTTGG AGGTGATTGA GATCCAGGGA 
CGTGCTGCAC TAGGTGCTCC AGCTCGAGCC CATCTTGATA TTCCCAACCC ACGTTACCTG 
GGACCAGCAA TCTCTTCTGG AGCCATTTAT ATTGCATCTG TTTACCAGGA TAAGCTGAGA 
GTAATCTGTT GCAAGGGAAA TCTAGTAAAG GACACTACAA GTGAACTTCA TAGGGCTCCT 
TCTACAACTC GCAGCCCAAA CAAGAGAGGA CCACCATCTT ACAATGACCA TATAACAAAG 
CGAATAGCAT CCAGCCCAGT GCCAACTGAG GTCCAAGGTC TCGTACGGGA GCCTAGTACA 
CCTCATAGAT ACCGCGAAGG ACGGACAGAA ATGCGCAGAG ACAAATCTCC TGGGAGGCCT 
TTGGACAGGG AGAAGTCTCC AGGGAGGTTA CTGAGCACCA GACGGGAGAG GTCACCTGGA 
AGGCTTTTTG AAGAGAGCAA CAGGAGCCGT GGAGCTGTAA GGACACCCTT GTCACAAGTC 
AATAAGGTTT GGGACCAGTC TTCTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cit ( XM_012956619.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812073.1 Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X1, mRNA.

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CloneID	OXa32035
Clone ID Related Accession (Same CDS sequence)	XM_012956619.2
Accession Version	XM_012956619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	citron Rho-interacting kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956619.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAA 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCTAAA TGACTTGGAA AAGAAGCATG CAATGCTGGA AATGAATGCT 
CGCAGTTTGC AACAAAAACT GGAGACTGAA AGAGAACTAA AGCAACGGCT TTTAGAAGAG 
CAGGCTAAGT TGCAACAGCA GATGGACCTG CAAAAGAATC ACATTTTCCG CCTGACCCAA 
GGACTTCAGG AGGCCCTGGA TCGAGCTGAT CTGCTTAAAA CAGAAAGAAG TGATTTGGAG 
TATCAACTGG AAAACATGCA GGCACTCTTT TCTCATGAAA AAGTAAAGAT GGAAGGAACC 
ATTTCCCAAC AAACAAAACT CATTGACTTC TTACAAGCAA AAATGGATCA GCCATCTAAG 
AAGAAAAAGG TACAAAATGG TTTGTTCAGT AGGCGCAGAG ATGAACCCAA TCAAACCTCA 
CAGGTTCCAT TACAGTACAA TGAGCTAAAA GTAGCGCTTG AAAAAGAGAA GGCTAGAACT 
GCAGAATTAG AAGAGGCTCT TCAAAAAACA CGAATTGAAC TCCGCTCTGC AAGAGAGGAA 
GCTGCTCACT TAAAGGTGTC AGATCATCCT GTTCCTTCTA CACCTGGTAC AGCAAGACAA 
CAGATTATCA TGTCTGCACT GGTCAGATCA CCTGAGCATC AGCCCAATGC AGTCAGCCTG 
CTTGCCCCAC AGAACAGCAG AAGGAAAGAA TCATCAGCTC CTGAAGAATT CAGTCGCCGA 
CTTAAAGAAA GAATGCATCA TAATATTCCA CACAGATTTA ATGTTGCACT TACCATGAGA 
GCAGCAAAAT GTTCGGTGTG CTTGGATACT GTTCACTTTG GGCGGCAGGG AGCAAAGTGC 
CTAGAATGTC AGGTCATGTG TCACCCAAAG TGTTCCTCCT GCCTCCCAGC TACATGTGGC 
CTCCCTGCAG AATATGCTTC ACATTTCACT GAAGCCTTTT GTAGAGAGAA AATGAATTCG 
CCTGGAATGC AGACGAAGGA AGCAAATGGT GTTGTGCGAC TTGAAGGCTG GATGAAAATT 
CCGAGGAATA ACAAACGTGG CCAGCAAGGA TGGGACAGGA AATATGTGGT CTTGGAAGGG 
TCTAAAGTGC TGATCTATGA TACTGAATTA AAAGAAGGAG GACAACGGCC TTCAGAGGAG 
TTTGAGCTTT GTCTTCCTGA TGGAGATGTA ACCGTTCATG GTGCCGTTGG ATCTACAGAG 
CTTGTAAATA CTGCAAAATC AGATGTGCCA TACGTCATGA AGATTGAGTC TCATCCACAC 
ACAACTTGTT GGCCAGGGCG CACTATGTAC TTGTTAGCGC CAAGTTTTCC TGATAAGCAG 
CGTTGGGTTA ATGCACTGGA GTCTGTGGTT GCAGGAGGAA GGGTGTCCAG AGAAAAAGCA 
GAGGCTGATG CTAAACTGCT TGGAAACTCT TTGTTAAAAT TGGAGGGGGA AGACCGTCTT 
GATATAAATT GCACAATACC TCTAACTGAG CAGATTGTTT TGGTTGGCGC AGAGGAGGGT 
CTTTATGCTC TGAATGTGCT GAAGAATTCT CTCACTCACA TACCAGGTAT AGGAGCAGTA 
TTCCAGATTC ATATTGTTAA GGAGCTGGAT AAACTTCTTT TAATAGCAGG GGAAGAAAGG 
GCTCTATGCC TTGTTGATGT GAAGAAGGTG AAGCAGTCAT TGGCTCAGTC ACATCTACCA 
GCACAGCCAG AAATCTCAAT GACTGTTTTT GAAGCTGTTA AAGGATGTCA CCTTTTTGCT 
GCAGGAAAGA TTGAAAATGG GTTATGCATC TGTGCAGCCA TGTTAAATAA AGTTGTCATA 
CTGCGCTACA ATGAAAGTCT CGGCAAGTTT TGCATAAGAA AGGAAATTGA AACTTCTGAG 
CCATGCAGTT GCATTTATTT CACTGGCTAC AGTGTCATCA TTGGAACAAA CAAGTTCTAT 
GAGATTGAGA TGAAGCAGTA CACTCTGGAG GAGTTCTTGG ATAAAAATGA CCATTCCTTG 
GCTTCTGCTG TGTTTGCTTC ATCTTCCAAT AGCTTCCCAA TAGCTATAAT GCAAGTAAAC 
AGCACAGGCC AGAGAGAAGA ATACCTCCTT TGCTTCCATG AGTTCGGTGT ATTTGTTGAT 
TCTTATGGAA GACGTAGTCG TACTGAAGAT TTGAAATGGA GTCGGCTTCC TCTTGCATTT 
GCCTACAGGG AACCGTACTT ATTTGTGACC CATTTTAACT CTTTGGAGGT GATTGAGATC 
CAGGGACGTG CTGCACTAGG TGCTCCAGCT CGAGCCCATC TTGATATTCC CAACCCACGT 
TACCTGGGAC CAGCAATCTC TTCTGGAGCC ATTTATATTG CATCTGTTTA CCAGGATAAG 
CTGAGAGTAA TCTGTTGCAA GGGAAATCTA GTAAAGGACA CTACAAGTGA ACTTCATAGG 
GCTCCTTCTA CAACTCGCAG CCCAAACAAG AGAGGACCAC CATCTTACAA TGACCATATA 
ACAAAGCGAA TAGCATCCAG CCCAGTGCCA ACTGAGGTCC AAGGTCTCGT ACGGGAGCCT 
AGTACACCTC ATAGATACCG CGAAGGACGG ACAGAAATGC GCAGAGACAA ATCTCCTGGG 
AGGCCTTTGG ACAGGGAGAA GTCTCCAGGG AGGTTACTGA GCACCAGACG GGAGAGGTCA 
CCTGGAAGGC TTTTTGAAGA GAGCAACAGG AGCCGTGGAG CTGTAAGGAC ACCCTTGTCA 
CAAGTCAATA AGGTTTGGGA CCAGTCTTCT GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812073.1
CDS	876..7091
Translation	MLKFKFGSRSAADAISSDPITSRAARLNNFFQGKSSCQFPFQQQLNVLSREGLLDSLCALYEECCNPALMKIKHVSNFVKKYSDTVAELRELQPNVKDFEIKNVVGRGHFAEVHVVKEKVTGDIFAMKVMKKKALLAQDQVSFFEEERNILSHHNSPWIPQLHYAFQDKENLYLVMQYQPGGDLFALMNRYDEQFDENMAQFYLAELVLAIYSVHQMGYVHRDIKPENILIDRTGHIKLVDFGSAAKLTANKTVNAKLPVGTPDYIAPEVLMVMNGDGRSTYGPECDWWSLGIVAYEMMYGKSPFTEGTSIKTFNNIMNFQRFLKFPEEPKLSSNFLDLIQSLICGQKERLKYEDLCCHPFFSNTDWDRLRNTPPPFVPTLKSDDDTSNFDEPEKNLRILPSSCQLNSAGFSGDDLPFVGFSFIKALSLLNKSESVVSGLDSPAKVSSMEKKLFHKSKELQDTQDKCHKMEQEMTRLHRRVSEVEAVLSQKEVELKASETQRSLLEQDLATYITECSSLKRSLEQARMEVSQEDDKALQLLNDIREQSRKLQDIKEQEFQAQLEEMKMMMHQLEEDLLAARRRSDLYETELRDSRLAAEEFKRKAVECQQKLQKAKDHGKGEVGELHTKLEKVNADQQNKIQELQEKLAKAVKASSEATELLQNIRQAKERAEKELEKLKNRDDSNDAIKKKLLDAEERRHSLENQVRRLETVERRENKLKEDIQTKSEQIQQMAEKIMELEERYREAQVGAQRLEVHLKQNEQVYEEKARNIETQMKKDLADKEALELSRIKNEEEICEKNRILSEQKAMINALESKITSLEQRLAELSEANKLAANSSLFTQRNMKAQEEMISELRQQKFYLETQAGKIEAQNQKLEEQLERINNQDHTDKNRLLELETRLREISLEHEEQKLELKRQLTEVQLTLQERETQISSLQSARSALESQLHQAKTELEDTTAEAEEEIQVLRSHRDEIQRKFDTLRDSCTVLTELEDQLNRISQENADLNNQNFYLSKQLSEVSGATDEIVQLRSEVDHLRREITEREMQLTSQKQTMEALKTTCTMLEEQVLDLEALNDELLEKERQWESWRSSLEDEKTQFECRLREVQRLLDTEKQSRVRSDQRTTESRQMVELAAKEHKAEILALQQALKDQKLKTESLSDKLNDLEKKHAMLEMNARSLQQKLETERELKQRLLEEQAKLQQQMDLQKNHIFRLTQGLQEALDRADLLKTERSDLEYQLENMQALFSHEKVKMEGTISQQTKLIDFLQAKMDQPSKKKKVQNGLFSRRRDEPNQTSQVPLQYNELKVALEKEKARTAELEEALQKTRIELRSAREEAAHLKVSDHPVPSTPGTARQQIIMSALVRSPEHQPNAVSLLAPQNSRRKESSAPEEFSRRLKERMHHNIPHRFNVALTMRAAKCSVCLDTVHFGRQGAKCLECQVMCHPKCSSCLPATCGLPAEYASHFTEAFCREKMNSPGMQTKEANGVVRLEGWMKIPRNNKRGQQGWDRKYVVLEGSKVLIYDTELKEGGQRPSEEFELCLPDGDVTVHGAVGSTELVNTAKSDVPYVMKIESHPHTTCWPGRTMYLLAPSFPDKQRWVNALESVVAGGRVSREKAEADAKLLGNSLLKLEGEDRLDINCTIPLTEQIVLVGAEEGLYALNVLKNSLTHIPGIGAVFQIHIVKELDKLLLIAGEERALCLVDVKKVKQSLAQSHLPAQPEISMTVFEAVKGCHLFAAGKIENGLCICAAMLNKVVILRYNESLGKFCIRKEIETSEPCSCIYFTGYSVIIGTNKFYEIEMKQYTLEEFLDKNDHSLASAVFASSSNSFPIAIMQVNSTGQREEYLLCFHEFGVFVDSYGRRSRTEDLKWSRLPLAFAYREPYLFVTHFNSLEVIEIQGRAALGAPARAHLDIPNPRYLGPAISSGAIYIASVYQDKLRVICCKGNLVKDTTSELHRAPSTTRSPNKRGPPSYNDHITKRIASSPVPTEVQGLVREPSTPHRYREGRTEMRRDKSPGRPLDREKSPGRLLSTRRERSPGRLFEESNRSRGAVRTPLSQVNKVWDQSSV

Target ORF information:

RefSeq Version	XM_012956619.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis citron rho-interacting serine/threonine kinase (cit), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956619.2

ORF Insert Sequence:

ATGCTGAAAT TCAAGTTTGG AAGCCGCAGT GCTGCGGATG CCATTTCTTC TGACCCCATT 
ACAAGCAGGG CAGCACGGCT TAACAACTTC TTTCAGGGAA AGTCATCATG CCAGTTTCCT 
TTTCAACAAC AATTAAATGT CCTGAGTCGA GAAGGTCTTT TAGATTCCTT ATGTGCTCTT 
TATGAAGAAT GTTGCAACCC TGCACTGATG AAGATTAAAC ATGTATCAAA CTTTGTAAAG 
AAATATTCTG ACACTGTTGC AGAGTTACGA GAGCTACAAC CCAATGTAAA AGACTTTGAG 
ATCAAGAATG TTGTTGGACG CGGTCATTTT GCAGAAGTGC ATGTAGTTAA AGAGAAAGTG 
ACAGGAGACA TCTTTGCTAT GAAAGTGATG AAGAAGAAAG CTCTCCTTGC ACAAGATCAG 
GTTTCTTTTT TTGAAGAAGA GAGGAATATA TTAAGCCATC ATAACAGTCC TTGGATTCCT 
CAGTTGCATT ATGCATTCCA AGACAAAGAA AATTTGTATT TGGTTATGCA ATACCAGCCA 
GGAGGAGACT TGTTTGCACT GATGAATCGC TATGATGAAC AGTTTGATGA AAACATGGCA 
CAGTTTTATC TAGCAGAATT AGTTCTTGCA ATTTACAGTG TTCATCAGAT GGGTTATGTG 
CATAGAGATA TTAAACCTGA AAACATCCTT ATTGACCGCA CTGGACATAT TAAGCTTGTT 
GACTTTGGAT CAGCTGCAAA ACTAACAGCA AATAAGACTG TAAATGCAAA GCTTCCTGTT 
GGCACTCCAG ATTACATTGC TCCAGAAGTT CTCATGGTAA TGAATGGAGA TGGCAGATCA 
ACATATGGTC CTGAATGTGA CTGGTGGTCT TTAGGCATTG TTGCCTATGA AATGATGTAT 
GGAAAATCAC CCTTCACAGA AGGGACTTCA ATAAAAACAT TCAATAACAT CATGAATTTT 
CAGCGTTTTT TAAAGTTTCC AGAAGAACCG AAACTCAGTA GTAATTTTCT TGATCTGATT 
CAGTCTTTGA TCTGTGGACA GAAAGAGAGG CTGAAATATG AAGATCTTTG CTGTCATCCA 
TTTTTTTCTA ACACTGACTG GGATAGGTTA CGAAACACTC CTCCTCCATT CGTTCCTACC 
TTAAAGTCTG ATGATGACAC CTCAAATTTT GATGAACCGG AGAAGAATTT GCGGATTTTA 
CCTTCTTCAT GCCAGCTGAA TTCTGCAGGA TTCTCGGGTG ACGATTTACC ATTTGTGGGG 
TTTTCATTCA TAAAGGCTCT GAGCTTGCTT AATAAATCTG AGTCTGTTGT ATCTGGCCTG 
GACTCTCCAG CCAAGGTGAG CTCCATGGAG AAGAAGTTAT TTCACAAGAG TAAAGAGCTT 
CAGGACACAC AGGACAAATG CCACAAGATG GAGCAAGAAA TGACGCGACT TCACCGACGG 
GTTTCAGAGG TGGAGGCTGT TCTCAGCCAA AAGGAGGTTG AGCTGAAAGC ATCTGAAACT 
CAGAGATCTC TTTTGGAACA GGACTTGGCA ACCTACATAA CAGAATGCAG CAGTTTAAAG 
CGCAGCCTCG AACAGGCCCG AATGGAAGTT TCCCAGGAGG ATGATAAGGC TCTGCAACTA 
CTGAATGATA TACGAGAACA GAGCAGAAAG CTTCAGGATA TAAAGGAGCA GGAGTTTCAA 
GCTCAGTTAG AAGAGATGAA GATGATGATG CACCAACTGG AAGAAGATCT TTTAGCTGCC 
CGGAGACGGA GTGATCTTTA TGAAACAGAA CTACGAGACT CCAGATTAGC GGCAGAAGAA 
TTTAAACGTA AAGCAGTGGA ATGTCAACAA AAGCTTCAGA AGGCTAAAGA TCATGGCAAA 
GGTGAAGTTG GAGAACTGCA TACTAAGCTG GAAAAGGTCA ATGCTGACCA ACAGAACAAA 
ATTCAAGAGT TGCAGGAAAA ATTGGCAAAG GCTGTGAAAG CCAGTTCAGA AGCTACAGAA 
TTGCTCCAAA ACATCCGCCA AGCAAAAGAG AGAGCAGAGA AAGAGCTTGA GAAACTCAAG 
AACCGAGATG ATTCAAATGA TGCAATAAAG AAAAAACTGC TTGATGCTGA GGAAAGACGT 
CATTCCCTAG AAAACCAAGT AAGAAGACTG GAGACAGTAG AACGTAGGGA AAACAAGTTG 
AAGGAAGATA TTCAGACAAA ATCTGAACAA ATTCAGCAAA TGGCTGAAAA GATTATGGAA 
CTTGAAGAAA GATACAGAGA GGCCCAGGTG GGAGCACAGC GTTTGGAAGT TCATCTAAAA 
CAGAACGAAC AAGTCTATGA AGAAAAGGCC AGAAATATTG AAACTCAAAT GAAAAAAGAC 
CTTGCTGATA AAGAGGCCCT TGAATTATCT CGTATAAAAA ATGAAGAAGA GATTTGTGAG 
AAAAACAGAA TCCTTAGTGA ACAAAAAGCG ATGATAAATG CTCTGGAATC AAAGATCACG 
TCCTTGGAAC AAAGGTTAGC AGAATTATCT GAAGCTAATA AACTGGCAGC TAATAGCAGT 
CTTTTTACAC AAAGGAACAT GAAAGCCCAA GAGGAGATGA TTTCAGAACT CAGGCAGCAA 
AAGTTTTACC TGGAAACACA GGCAGGAAAA ATAGAGGCTC AGAATCAGAA ACTGGAGGAA 
CAGCTAGAGA GAATAAATAA TCAGGACCAC ACTGACAAAA ACCGCCTTCT AGAACTGGAG 
ACAAGGCTGC GAGAGATTAG TTTGGAGCAT GAAGAACAAA AGCTGGAGCT AAAGAGACAG 
TTAACAGAGG TGCAGCTCAC ATTACAGGAA AGAGAAACCC AGATCTCCAG TTTGCAATCT 
GCACGTTCTG CATTAGAGAG TCAGCTACAT CAGGCAAAAA CAGAGCTGGA AGATACAACT 
GCAGAGGCTG AGGAAGAGAT TCAAGTTTTA AGGTCTCACA GAGATGAGAT CCAGCGCAAA 
TTTGATACTT TGCGTGACAG TTGTACGGTC CTTACAGAGC TAGAGGATCA GCTTAATCGT 
ATCAGTCAAG AAAATGCAGA TTTGAACAAT CAAAACTTTT ATTTGTCAAA GCAACTTAGT 
GAGGTTTCAG GAGCCACTGA TGAAATTGTG CAACTGCGAA GTGAAGTGGA TCATCTCAGA 
AGAGAAATCA CAGAAAGAGA GATGCAACTC ACAAGTCAAA AGCAGACCAT GGAGGCCCTG 
AAAACTACAT GTACCATGCT AGAGGAACAA GTGCTGGATC TTGAAGCATT GAATGATGAG 
TTGCTAGAAA AGGAACGTCA GTGGGAGAGC TGGCGTAGTT CCCTAGAAGA TGAGAAAACT 
CAGTTTGAAT GTAGACTTCG AGAGGTACAG AGATTGCTGG ATACCGAAAA ACAGAGCAGG 
GTACGATCTG ACCAACGAAC TACAGAATCA CGCCAGATGG TGGAATTAGC TGCCAAGGAA 
CACAAGGCTG AAATCTTAGC TTTACAGCAA GCACTGAAAG ATCAGAAGCT CAAGACAGAA 
AGTCTTTCAG ATAAGCTAAA TGACTTGGAA AAGAAGCATG CAATGCTGGA AATGAATGCT 
CGCAGTTTGC AACAAAAACT GGAGACTGAA AGAGAACTAA AGCAACGGCT TTTAGAAGAG 
CAGGCTAAGT TGCAACAGCA GATGGACCTG CAAAAGAATC ACATTTTCCG CCTGACCCAA 
GGACTTCAGG AGGCCCTGGA TCGAGCTGAT CTGCTTAAAA CAGAAAGAAG TGATTTGGAG 
TATCAACTGG AAAACATGCA GGCACTCTTT TCTCATGAAA AAGTAAAGAT GGAAGGAACC 
ATTTCCCAAC AAACAAAACT CATTGACTTC TTACAAGCAA AAATGGATCA GCCATCTAAG 
AAGAAAAAGG TACAAAATGG TTTGTTCAGT AGGCGCAGAG ATGAACCCAA TCAAACCTCA 
CAGGTTCCAT TACAGTACAA TGAGCTAAAA GTAGCGCTTG AAAAAGAGAA GGCTAGAACT 
GCAGAATTAG AAGAGGCTCT TCAAAAAACA CGAATTGAAC TCCGCTCTGC AAGAGAGGAA 
GCTGCTCACT TAAAGGTGTC AGATCATCCT GTTCCTTCTA CACCTGGTAC AGCAAGACAA 
CAGATTATCA TGTCTGCACT GGTCAGATCA CCTGAGCATC AGCCCAATGC AGTCAGCCTG 
CTTGCCCCAC AGAACAGCAG AAGGAAAGAA TCATCAGCTC CTGAAGAATT CAGTCGCCGA 
CTTAAAGAAA GAATGCATCA TAATATTCCA CACAGATTTA ATGTTGCACT TACCATGAGA 
GCAGCAAAAT GTTCGGTGTG CTTGGATACT GTTCACTTTG GGCGGCAGGG AGCAAAGTGC 
CTAGAATGTC AGGTCATGTG TCACCCAAAG TGTTCCTCCT GCCTCCCAGC TACATGTGGC 
CTCCCTGCAG AATATGCTTC ACATTTCACT GAAGCCTTTT GTAGAGAGAA AATGAATTCG 
CCTGGAATGC AGACGAAGGA AGCAAATGGT GTTGTGCGAC TTGAAGGCTG GATGAAAATT 
CCGAGGAATA ACAAACGTGG CCAGCAAGGA TGGGACAGGA AATATGTGGT CTTGGAAGGG 
TCTAAAGTGC TGATCTATGA TACTGAATTA AAAGAAGGAG GACAACGGCC TTCAGAGGAG 
TTTGAGCTTT GTCTTCCTGA TGGAGATGTA ACCGTTCATG GTGCCGTTGG ATCTACAGAG 
CTTGTAAATA CTGCAAAATC AGATGTGCCA TACGTCATGA AGATTGAGTC TCATCCACAC 
ACAACTTGTT GGCCAGGGCG CACTATGTAC TTGTTAGCGC CAAGTTTTCC TGATAAGCAG 
CGTTGGGTTA ATGCACTGGA GTCTGTGGTT GCAGGAGGAA GGGTGTCCAG AGAAAAAGCA 
GAGGCTGATG CTAAACTGCT TGGAAACTCT TTGTTAAAAT TGGAGGGGGA AGACCGTCTT 
GATATAAATT GCACAATACC TCTAACTGAG CAGATTGTTT TGGTTGGCGC AGAGGAGGGT 
CTTTATGCTC TGAATGTGCT GAAGAATTCT CTCACTCACA TACCAGGTAT AGGAGCAGTA 
TTCCAGATTC ATATTGTTAA GGAGCTGGAT AAACTTCTTT TAATAGCAGG GGAAGAAAGG 
GCTCTATGCC TTGTTGATGT GAAGAAGGTG AAGCAGTCAT TGGCTCAGTC ACATCTACCA 
GCACAGCCAG AAATCTCAAT GACTGTTTTT GAAGCTGTTA AAGGATGTCA CCTTTTTGCT 
GCAGGAAAGA TTGAAAATGG GTTATGCATC TGTGCAGCCA TGTTAAATAA AGTTGTCATA 
CTGCGCTACA ATGAAAGTCT CGGCAAGTTT TGCATAAGAA AGGAAATTGA AACTTCTGAG 
CCATGCAGTT GCATTTATTT CACTGGCTAC AGTGTCATCA TTGGAACAAA CAAGTTCTAT 
GAGATTGAGA TGAAGCAGTA CACTCTGGAG GAGTTCTTGG ATAAAAATGA CCATTCCTTG 
GCTTCTGCTG TGTTTGCTTC ATCTTCCAAT AGCTTCCCAA TAGCTATAAT GCAAGTAAAC 
AGCACAGGCC AGAGAGAAGA ATACCTCCTT TGCTTCCATG AGTTCGGTGT ATTTGTTGAT 
TCTTATGGAA GACGTAGTCG TACTGAAGAT TTGAAATGGA GTCGGCTTCC TCTTGCATTT 
GCCTACAGGG AACCGTACTT ATTTGTGACC CATTTTAACT CTTTGGAGGT GATTGAGATC 
CAGGGACGTG CTGCACTAGG TGCTCCAGCT CGAGCCCATC TTGATATTCC CAACCCACGT 
TACCTGGGAC CAGCAATCTC TTCTGGAGCC ATTTATATTG CATCTGTTTA CCAGGATAAG 
CTGAGAGTAA TCTGTTGCAA GGGAAATCTA GTAAAGGACA CTACAAGTGA ACTTCATAGG 
GCTCCTTCTA CAACTCGCAG CCCAAACAAG AGAGGACCAC CATCTTACAA TGACCATATA 
ACAAAGCGAA TAGCATCCAG CCCAGTGCCA ACTGAGGTCC AAGGTCTCGT ACGGGAGCCT 
AGTACACCTC ATAGATACCG CGAAGGACGG ACAGAAATGC GCAGAGACAA ATCTCCTGGG 
AGGCCTTTGG ACAGGGAGAA GTCTCCAGGG AGGTTACTGA GCACCAGACG GGAGAGGTCA 
CCTGGAAGGC TTTTTGAAGA GAGCAACAGG AGCCGTGGAG CTGTAAGGAC ACCCTTGTCA 
CAAGTCAATA AGGTTTGGGA CCAGTCTTCT GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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cit cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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