plch2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

  • Gene

  • Clones

  • gRNAs

The following plch2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plch2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa45898 XM_018096047.1
Latest version!
Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa61848 XM_031905493.1
Latest version!
Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa45896 XM_018096045.1
Latest version!
Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa45897 XM_018096046.1
Latest version!
Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa61844 XM_031905490.1
Latest version!
Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa61845 XM_031905491.1
Latest version!
Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa61846 XM_018096045.2
Latest version!
Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa61847 XM_031905492.1
Latest version!
Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa45898
    Clone ID Related Accession (Same CDS sequence) XM_018096047.1
    Accession Version XM_018096047.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
    AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA
    GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA
    AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA
    CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT
    ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT
    CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT
    TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG
    AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC
    AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA
    GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC
    CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA
    AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT
    ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT
    GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA
    GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA
    TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG
    CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA
    GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT
    AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC
    AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA
    AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG
    GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA
    GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT
    GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT
    GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC
    ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC
    TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA
    GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG
    AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC
    CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA
    ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC
    CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA
    GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC
    TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT
    TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG
    TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC
    ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA
    CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG
    GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG
    GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG
    GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG
    GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG
    ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT
    GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT
    CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA
    CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA
    AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA
    CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA
    AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT
    TTAAAAGTTA AAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951536.1
    CDS89..3106
    Translation

    Target ORF information:

    RefSeq Version XM_018096047.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096047.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
    AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA
    GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA
    AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA
    CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT
    ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT
    CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT
    TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG
    AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC
    AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA
    GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC
    CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA
    AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT
    ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT
    GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA
    GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA
    TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG
    CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA
    GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT
    AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC
    AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA
    AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG
    GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA
    GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT
    GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT
    GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC
    ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC
    TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA
    GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG
    AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC
    CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA
    ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC
    CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA
    GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC
    TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT
    TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG
    TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC
    ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA
    CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG
    GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG
    GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG
    GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG
    GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG
    ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT
    GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT
    CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA
    CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA
    AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA
    CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA
    AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT
    TTAAAAGTTA AAGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61848
    Clone ID Related Accession (Same CDS sequence) XM_031905493.1
    Accession Version XM_031905493.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3072bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
    GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC
    ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG
    GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG
    AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT
    GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC
    TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA
    TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG
    CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA
    GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC
    CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG
    CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC
    CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA
    TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT
    GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC
    ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT
    CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC
    ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC
    GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT
    CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA
    TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG
    GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC
    TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA
    ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT
    GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT
    TGGAAGGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG
    CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA
    CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA
    TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG
    AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA
    GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG
    GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG
    AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT
    GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC
    CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC
    ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT
    GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC
    AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT
    CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG
    GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT
    CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC
    TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC
    CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT
    GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA
    GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA
    ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA
    AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC
    ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC
    AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT
    GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT
    TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA
    GTTAAAGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761353.1
    CDS525..3596
    Translation

    Target ORF information:

    RefSeq Version XM_031905493.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905493.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
    GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC
    ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG
    GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG
    AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT
    GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC
    TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA
    TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG
    CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA
    GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC
    CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG
    CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC
    CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA
    TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT
    GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC
    ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT
    CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC
    ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC
    GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT
    CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA
    TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG
    GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC
    TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA
    ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT
    GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT
    TGGAAGGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG
    CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA
    CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA
    TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG
    AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA
    GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG
    GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG
    AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT
    GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC
    CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC
    ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT
    GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC
    AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT
    CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG
    GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT
    CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC
    TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC
    CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT
    GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA
    GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA
    ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA
    AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC
    ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC
    AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT
    GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT
    TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA
    GTTAAAGACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45896
    Clone ID Related Accession (Same CDS sequence) XM_018096045.1
    Accession Version XM_018096045.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
    AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA
    GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA
    AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA
    CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT
    ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT
    CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT
    TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG
    AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC
    AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA
    GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC
    CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA
    AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT
    ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT
    GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA
    GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA
    TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG
    CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA
    GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT
    AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC
    AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA
    AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG
    GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA
    GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT
    GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT
    GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC
    ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC
    TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA
    GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG
    AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC
    CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA
    ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC
    CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA
    GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC
    TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT
    TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG
    TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC
    ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA
    CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG
    GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG
    GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG
    GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG
    GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG
    ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT
    GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT
    CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA
    CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA
    AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA
    CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA
    AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT
    TTAAAAGACC CTCGACAATT ATCAATTTAT AGATCGGCCT CCTCTTTATG CAGTCTTGAA
    ACCATTAAAG AAGAGCCTCT CTTTGCCAAG GAAAAACCTT CCACTAGTCC CCAGCCTGTT
    GCCTGTTTTA TAAGTTGTGA AAGCACAGCA TGTGATCAGT ACAAGGAATC TCAGTCTGAA
    TGCAGTAGCC CAGCTATTAC CTCTGAGAAT GTACAACGAC CAAGTAAACT GAATGTTGAG
    CCAGCCATAA TCAAGGTGCT TGCTATAAGT AATGGACTAC ATCCACATAA TGGAAAACTT
    AATCCAGGAA AAGCATACAA TGACAGGAAT GGAAAAGTAC TCAGTTCTGT CAGTGGGAAA
    AGTGATTGCA CAAATCTAAT TAATTCATCC CTGATTAAGC CTGGGTTGTC TTTACTTGCT
    GATGTTTCAA GAAATGTCAA TGAACAATAT CAAGTTAAAG CTAACCAAGC TGCACAATCT
    TCCATTCATG CTAGTAATAC TTCAGTTGCA AACAGAATAA TGTGCACTAA TCTACCTGAT
    CATCAAACTA GTTCAAAACC AAACATGAAA CATCTGATTG TTCCTCCTGT TCAAAGATCA
    CAGTATGACA GGCCTGACTC AAAACCTTCT GTGGTTCATG CTGGCAAACA CACACCTGTA
    AGGAGAGCAA AGAGTGAGGG CTATGTTGTT TCAGACTGCT CAGCCTTAAG CTTAAATTCT
    ACATGTCAGT CCCCTGTCGC TGAAGTATAT TCTGATGCTA CAGTTAATGA TAGAATTTGG
    AGCAAACTGG AGTTAAACTC TCATAGGGAC AGTATGTCTT CGTCTTCCAG CAAGTCATCC
    AATGACACAG TCATTGATCT GTCTTTGCCT AATTTAGCCC GGAAAAGCTT ATTGGATCTT
    GACATCTCTG ACGATAACTT TGAAAATATA CATTTAAACA ATACTATTCG GCCAAGATCC
    GCTACATCTT CAACAAAGGA AATGTCGCTA GGAGTTTCAA AAAGTAAGTC TAACCCTAAT
    TTAGTATCCA ATAACCAAGC AATAGATGAA CTCTCTTCTA GACCACTGGC TAGAAGTCCT
    ACAGAAGCAT TGCCACACTT GCCACGAAGG AACACCTGGA ATAGATTATA CGTAGATAGT
    TTAAAACAGA CAAATAAAAA GCCCAGTTTT GAGAATAAAC CACCAACTAA CCAGTCAATG
    TCGAAAAGTC TTGGAGATCT GACTTCAGAA GATATTTCAT GTAATTTTGA AAGTAAGTAC
    AGAAGTATAA GCAGAAGTTT TATTACTAGA TCAATGAGGG CACAAAGAAG ACCGGCCAGT
    ATGAAGGGTT CAGAGAAGTC TTCCGATTCA CTGACCGAAC AGCTGAAAAA GCTAACAAGC
    TTTCAGAAAG AAAATGATAT TACATCCCCA ACTGATTTTG AACCTGTTCA TTGTAACAAT
    GATGACACAG CCTTCCTTCG AAGGACGTCC TCCAGAAGTC AGAGTAGAGT AAGATATATT
    GCAAACCGAG CCAAGCAGGC TCAAGAAAGG CAAAGGCTCC AAAACCTGTG CCGGATAAAT
    GGAAATTCCA TAGAAGAAAG AGGGAATCCA GAAGGGGCAT GTGCAATAAC TAAAGGAATC
    TGTATGGATT CTCTTTCTCC CACACAGTTT ACATCTGAAA CATTGGACAA ATTTGGACCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951534.1
    CDS89..4771
    Translation

    Target ORF information:

    RefSeq Version XM_018096045.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096045.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
    AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA
    GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA
    AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA
    CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT
    ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT
    CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT
    TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG
    AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC
    AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA
    GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC
    CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA
    AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT
    ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT
    GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA
    GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA
    TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG
    CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA
    GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT
    AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC
    AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA
    AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG
    GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA
    GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT
    GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT
    GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC
    ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC
    TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA
    GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG
    AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC
    CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA
    ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC
    CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA
    GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC
    TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT
    TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG
    TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC
    ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA
    CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG
    GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG
    GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG
    GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG
    GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG
    ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT
    GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT
    CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA
    CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA
    AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA
    CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA
    AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT
    TTAAAAGACC CTCGACAATT ATCAATTTAT AGATCGGCCT CCTCTTTATG CAGTCTTGAA
    ACCATTAAAG AAGAGCCTCT CTTTGCCAAG GAAAAACCTT CCACTAGTCC CCAGCCTGTT
    GCCTGTTTTA TAAGTTGTGA AAGCACAGCA TGTGATCAGT ACAAGGAATC TCAGTCTGAA
    TGCAGTAGCC CAGCTATTAC CTCTGAGAAT GTACAACGAC CAAGTAAACT GAATGTTGAG
    CCAGCCATAA TCAAGGTGCT TGCTATAAGT AATGGACTAC ATCCACATAA TGGAAAACTT
    AATCCAGGAA AAGCATACAA TGACAGGAAT GGAAAAGTAC TCAGTTCTGT CAGTGGGAAA
    AGTGATTGCA CAAATCTAAT TAATTCATCC CTGATTAAGC CTGGGTTGTC TTTACTTGCT
    GATGTTTCAA GAAATGTCAA TGAACAATAT CAAGTTAAAG CTAACCAAGC TGCACAATCT
    TCCATTCATG CTAGTAATAC TTCAGTTGCA AACAGAATAA TGTGCACTAA TCTACCTGAT
    CATCAAACTA GTTCAAAACC AAACATGAAA CATCTGATTG TTCCTCCTGT TCAAAGATCA
    CAGTATGACA GGCCTGACTC AAAACCTTCT GTGGTTCATG CTGGCAAACA CACACCTGTA
    AGGAGAGCAA AGAGTGAGGG CTATGTTGTT TCAGACTGCT CAGCCTTAAG CTTAAATTCT
    ACATGTCAGT CCCCTGTCGC TGAAGTATAT TCTGATGCTA CAGTTAATGA TAGAATTTGG
    AGCAAACTGG AGTTAAACTC TCATAGGGAC AGTATGTCTT CGTCTTCCAG CAAGTCATCC
    AATGACACAG TCATTGATCT GTCTTTGCCT AATTTAGCCC GGAAAAGCTT ATTGGATCTT
    GACATCTCTG ACGATAACTT TGAAAATATA CATTTAAACA ATACTATTCG GCCAAGATCC
    GCTACATCTT CAACAAAGGA AATGTCGCTA GGAGTTTCAA AAAGTAAGTC TAACCCTAAT
    TTAGTATCCA ATAACCAAGC AATAGATGAA CTCTCTTCTA GACCACTGGC TAGAAGTCCT
    ACAGAAGCAT TGCCACACTT GCCACGAAGG AACACCTGGA ATAGATTATA CGTAGATAGT
    TTAAAACAGA CAAATAAAAA GCCCAGTTTT GAGAATAAAC CACCAACTAA CCAGTCAATG
    TCGAAAAGTC TTGGAGATCT GACTTCAGAA GATATTTCAT GTAATTTTGA AAGTAAGTAC
    AGAAGTATAA GCAGAAGTTT TATTACTAGA TCAATGAGGG CACAAAGAAG ACCGGCCAGT
    ATGAAGGGTT CAGAGAAGTC TTCCGATTCA CTGACCGAAC AGCTGAAAAA GCTAACAAGC
    TTTCAGAAAG AAAATGATAT TACATCCCCA ACTGATTTTG AACCTGTTCA TTGTAACAAT
    GATGACACAG CCTTCCTTCG AAGGACGTCC TCCAGAAGTC AGAGTAGAGT AAGATATATT
    GCAAACCGAG CCAAGCAGGC TCAAGAAAGG CAAAGGCTCC AAAACCTGTG CCGGATAAAT
    GGAAATTCCA TAGAAGAAAG AGGGAATCCA GAAGGGGCAT GTGCAATAAC TAAAGGAATC
    TGTATGGATT CTCTTTCTCC CACACAGTTT ACATCTGAAA CATTGGACAA ATTTGGACCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa45897
    Clone ID Related Accession (Same CDS sequence) XM_018096046.1
    Accession Version XM_018096046.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4677bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
    AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA
    GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA
    AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA
    CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT
    ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT
    CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT
    TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG
    AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC
    AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA
    GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC
    CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA
    AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT
    ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT
    GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA
    GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA
    TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG
    CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA
    GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT
    AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC
    AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA
    AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG
    GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA
    GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT
    GGCGATGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG
    CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA
    CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA
    TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG
    AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA
    GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG
    GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG
    AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT
    GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC
    CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC
    ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT
    GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC
    AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT
    CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG
    GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT
    CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC
    TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC
    CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT
    GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA
    GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA
    ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA
    AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC
    ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC
    AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT
    GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT
    TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA
    GACCCTCGAC AATTATCAAT TTATAGATCG GCCTCCTCTT TATGCAGTCT TGAAACCATT
    AAAGAAGAGC CTCTCTTTGC CAAGGAAAAA CCTTCCACTA GTCCCCAGCC TGTTGCCTGT
    TTTATAAGTT GTGAAAGCAC AGCATGTGAT CAGTACAAGG AATCTCAGTC TGAATGCAGT
    AGCCCAGCTA TTACCTCTGA GAATGTACAA CGACCAAGTA AACTGAATGT TGAGCCAGCC
    ATAATCAAGG TGCTTGCTAT AAGTAATGGA CTACATCCAC ATAATGGAAA ACTTAATCCA
    GGAAAAGCAT ACAATGACAG GAATGGAAAA GTACTCAGTT CTGTCAGTGG GAAAAGTGAT
    TGCACAAATC TAATTAATTC ATCCCTGATT AAGCCTGGGT TGTCTTTACT TGCTGATGTT
    TCAAGAAATG TCAATGAACA ATATCAAGTT AAAGCTAACC AAGCTGCACA ATCTTCCATT
    CATGCTAGTA ATACTTCAGT TGCAAACAGA ATAATGTGCA CTAATCTACC TGATCATCAA
    ACTAGTTCAA AACCAAACAT GAAACATCTG ATTGTTCCTC CTGTTCAAAG ATCACAGTAT
    GACAGGCCTG ACTCAAAACC TTCTGTGGTT CATGCTGGCA AACACACACC TGTAAGGAGA
    GCAAAGAGTG AGGGCTATGT TGTTTCAGAC TGCTCAGCCT TAAGCTTAAA TTCTACATGT
    CAGTCCCCTG TCGCTGAAGT ATATTCTGAT GCTACAGTTA ATGATAGAAT TTGGAGCAAA
    CTGGAGTTAA ACTCTCATAG GGACAGTATG TCTTCGTCTT CCAGCAAGTC ATCCAATGAC
    ACAGTCATTG ATCTGTCTTT GCCTAATTTA GCCCGGAAAA GCTTATTGGA TCTTGACATC
    TCTGACGATA ACTTTGAAAA TATACATTTA AACAATACTA TTCGGCCAAG ATCCGCTACA
    TCTTCAACAA AGGAAATGTC GCTAGGAGTT TCAAAAAGTA AGTCTAACCC TAATTTAGTA
    TCCAATAACC AAGCAATAGA TGAACTCTCT TCTAGACCAC TGGCTAGAAG TCCTACAGAA
    GCATTGCCAC ACTTGCCACG AAGGAACACC TGGAATAGAT TATACGTAGA TAGTTTAAAA
    CAGACAAATA AAAAGCCCAG TTTTGAGAAT AAACCACCAA CTAACCAGTC AATGTCGAAA
    AGTCTTGGAG ATCTGACTTC AGAAGATATT TCATGTAATT TTGAAAGTAA GTACAGAAGT
    ATAAGCAGAA GTTTTATTAC TAGATCAATG AGGGCACAAA GAAGACCGGC CAGTATGAAG
    GGTTCAGAGA AGTCTTCCGA TTCACTGACC GAACAGCTGA AAAAGCTAAC AAGCTTTCAG
    AAAGAAAATG ATATTACATC CCCAACTGAT TTTGAACCTG TTCATTGTAA CAATGATGAC
    ACAGCCTTCC TTCGAAGGAC GTCCTCCAGA AGTCAGAGTA GAGTAAGATA TATTGCAAAC
    CGAGCCAAGC AGGCTCAAGA AAGGCAAAGG CTCCAAAACC TGTGCCGGAT AAATGGAAAT
    TCCATAGAAG AAAGAGGGAA TCCAGAAGGG GCATGTGCAA TAACTAAAGG AATCTGTATG
    GATTCTCTTT CTCCCACACA GTTTACATCT GAAACATTGG ACAAATTTGG ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951535.1
    CDS89..4765
    Translation

    Target ORF information:

    RefSeq Version XM_018096046.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096046.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
    AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA
    GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA
    AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA
    CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT
    ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT
    CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT
    TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG
    AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC
    AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA
    GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC
    CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA
    AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT
    ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT
    GGTTTCACTA ACTATATGCG CAGTCCAGCG GGTGATATCT TCAATCCAGA CCACTATCAA
    GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA
    TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG
    CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA
    GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT
    AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC
    AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA
    AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG
    GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA
    GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT
    GGCGATGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG
    CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA
    CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA
    TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG
    AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA
    GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG
    GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG
    AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT
    GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC
    CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC
    ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT
    GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC
    AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT
    CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG
    GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT
    CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC
    TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC
    CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT
    GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA
    GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA
    ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA
    AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC
    ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC
    AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT
    GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT
    TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA
    GACCCTCGAC AATTATCAAT TTATAGATCG GCCTCCTCTT TATGCAGTCT TGAAACCATT
    AAAGAAGAGC CTCTCTTTGC CAAGGAAAAA CCTTCCACTA GTCCCCAGCC TGTTGCCTGT
    TTTATAAGTT GTGAAAGCAC AGCATGTGAT CAGTACAAGG AATCTCAGTC TGAATGCAGT
    AGCCCAGCTA TTACCTCTGA GAATGTACAA CGACCAAGTA AACTGAATGT TGAGCCAGCC
    ATAATCAAGG TGCTTGCTAT AAGTAATGGA CTACATCCAC ATAATGGAAA ACTTAATCCA
    GGAAAAGCAT ACAATGACAG GAATGGAAAA GTACTCAGTT CTGTCAGTGG GAAAAGTGAT
    TGCACAAATC TAATTAATTC ATCCCTGATT AAGCCTGGGT TGTCTTTACT TGCTGATGTT
    TCAAGAAATG TCAATGAACA ATATCAAGTT AAAGCTAACC AAGCTGCACA ATCTTCCATT
    CATGCTAGTA ATACTTCAGT TGCAAACAGA ATAATGTGCA CTAATCTACC TGATCATCAA
    ACTAGTTCAA AACCAAACAT GAAACATCTG ATTGTTCCTC CTGTTCAAAG ATCACAGTAT
    GACAGGCCTG ACTCAAAACC TTCTGTGGTT CATGCTGGCA AACACACACC TGTAAGGAGA
    GCAAAGAGTG AGGGCTATGT TGTTTCAGAC TGCTCAGCCT TAAGCTTAAA TTCTACATGT
    CAGTCCCCTG TCGCTGAAGT ATATTCTGAT GCTACAGTTA ATGATAGAAT TTGGAGCAAA
    CTGGAGTTAA ACTCTCATAG GGACAGTATG TCTTCGTCTT CCAGCAAGTC ATCCAATGAC
    ACAGTCATTG ATCTGTCTTT GCCTAATTTA GCCCGGAAAA GCTTATTGGA TCTTGACATC
    TCTGACGATA ACTTTGAAAA TATACATTTA AACAATACTA TTCGGCCAAG ATCCGCTACA
    TCTTCAACAA AGGAAATGTC GCTAGGAGTT TCAAAAAGTA AGTCTAACCC TAATTTAGTA
    TCCAATAACC AAGCAATAGA TGAACTCTCT TCTAGACCAC TGGCTAGAAG TCCTACAGAA
    GCATTGCCAC ACTTGCCACG AAGGAACACC TGGAATAGAT TATACGTAGA TAGTTTAAAA
    CAGACAAATA AAAAGCCCAG TTTTGAGAAT AAACCACCAA CTAACCAGTC AATGTCGAAA
    AGTCTTGGAG ATCTGACTTC AGAAGATATT TCATGTAATT TTGAAAGTAA GTACAGAAGT
    ATAAGCAGAA GTTTTATTAC TAGATCAATG AGGGCACAAA GAAGACCGGC CAGTATGAAG
    GGTTCAGAGA AGTCTTCCGA TTCACTGACC GAACAGCTGA AAAAGCTAAC AAGCTTTCAG
    AAAGAAAATG ATATTACATC CCCAACTGAT TTTGAACCTG TTCATTGTAA CAATGATGAC
    ACAGCCTTCC TTCGAAGGAC GTCCTCCAGA AGTCAGAGTA GAGTAAGATA TATTGCAAAC
    CGAGCCAAGC AGGCTCAAGA AAGGCAAAGG CTCCAAAACC TGTGCCGGAT AAATGGAAAT
    TCCATAGAAG AAAGAGGGAA TCCAGAAGGG GCATGTGCAA TAACTAAAGG AATCTGTATG
    GATTCTCTTT CTCCCACACA GTTTACATCT GAAACATTGG ACAAATTTGG ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61844
    Clone ID Related Accession (Same CDS sequence) XM_031905490.1
    Accession Version XM_031905490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
    GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC
    ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG
    GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG
    AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT
    GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC
    TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA
    TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG
    CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA
    GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC
    CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG
    CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC
    CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA
    TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT
    GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC
    ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT
    CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC
    ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC
    GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT
    CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA
    TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG
    GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC
    TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA
    ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT
    GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT
    TGGAAGGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG
    CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA
    CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA
    TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG
    AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA
    GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG
    GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG
    AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT
    GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC
    CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC
    ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT
    GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC
    AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT
    CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG
    GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT
    CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC
    TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC
    CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT
    GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA
    GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA
    ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA
    AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC
    ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC
    AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT
    GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT
    TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA
    GACCCTCGAC AATTATCAAT TTATAGATCG GCCTCCTCTT TATGCAGTCT TGAAACCATT
    AAAGAAGAGC CTCTCTTTGC CAAGGAAAAA CCTTCCACTA GTCCCCAGCC TGTTGCCTGT
    TTTACAAGTT GTGAAAGCAC AGCATGTGAT CAGTACAAGG AATCTCAGTC TGAATGCAGT
    AGCCCAGCTA TTACCTCTGA GAATGTACAA CGACCAAGTA AACTGAATGT TGAGCCAGCC
    ATAATCAAGG TGCTTGCTAT AAGTAATGGA CTACATCCAC ATAATGGAAA ACTTAATCCA
    GGAAAAGCAT ACAATGACAG GAATGGAAAA GTACTCAGTT CTGTCAGTGG GAAAAGTGAT
    TGCACAAATC TAATTAATTC ATCCCTGATT AAGCCTGGGT TGTCTTTACT TGCTGATGTT
    TCAAGAAATG TCAATGAACA ATATCAAGTT AAAGCTAACC AAGCTGCACA ATCTTCCATT
    CATGCTAGTA ATACTTCAGT TGCAAACAGA ATAATGTGCA CTAATCTACC TGATCATCAA
    ACTAGTTCAA AACCAAACAT GAAACATCTG ATTGTTCCTC CTGTTCAAAG ATCACAGTAT
    GACAGGCCTG ACTCAAAACC TTCTGTGGTT CATGCTGGCA AACACACACC TGTAAGGAGA
    GCAAAGAGTG AGGGCTATGT TGTTTCAGAC TGCTCAGCCT TAAGCTTAAA TTCTACATGT
    CAGTCCCCTG TCGCTGAAGT ATATTCTGAT GCTACAGTTA ATGATAGAAT TTGGAGCAAA
    CTGGAGTTAA ACTCTCATAG GGACAGTATG TCTTCGTCTT CCAGCAAGTC ATCCAATGAC
    ACAGTCATTG ATCTGTCTTT GCCTAATTTA GCCCGGAAAA GCTTATTGGA TCTTGACATC
    TCTGATGATA ACTTTGAAAA TATACATTTA AACAATACTA TTCGGCCAAG ATCCGCTACA
    TCTTCAACAA AGGAAATGTC GCTAGGAGTT TCAAAAAGTA AGTCTAACCC TAATTTAGTA
    TCCAATAACC AAGCAATAGA TGAACTCTCT TCTAGACCAC TGGCTAGAAG TCCTACAGAA
    GCATTGCCAC ACTTGCCACG AAGGAACACC TGGAATAGAT TATACGTAGA TAGTTTAAAA
    CAGGCAAATA AAAAGCCCAG TTTTGAGAAT AAACCACCAA CTAACCAGTC AATGTCGAAA
    AGTCTTGGAG ATCTGACTTC AGAAGATATT TCATGTAATT TTGAAAGTAA GTACAGAAGT
    ATAAGCAGAA GTTTTATTAC TAGATCAATG AGGGCACAAA GAAGACCGGC CAGTATGAAG
    GGTTCAGAGA AGTCTTCCGA TTCACTGACC GAACAGCTGA AAAAGCTAAC AAGCTTTCAG
    AAAGAAAATG ATATTACATC CCCAACTGAT TTTGAACCTG TTCATTGTAA CAATGATGAC
    ACAGCCTTCC TTCGAAGGAC GTCCTCCAGA AGTCAGAGTA GAGTAAGATA TATTGCAAAC
    CGAGCCAAGC AGGCTCAAGA AAGGCAAAGG CTCCAAAACC TGTGCCGGAT AAATGGAAAT
    TCCATAGAAG AAAGAGGGAA TCCAGAAGGG GCATGTGCAA TAACTAAAGG AATCTGTATG
    GATTCTCTTT CTCCCACACA GTTTACATCT GAAACATTGG ACAAATTTGG ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761350.1
    CDS524..5260
    Translation

    Target ORF information:

    RefSeq Version XM_031905490.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
    GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC
    ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG
    GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG
    AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT
    GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC
    TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA
    TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG
    CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA
    GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC
    CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG
    CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC
    CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA
    TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT
    GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC
    ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT
    CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC
    ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC
    GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT
    CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA
    TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG
    GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC
    TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA
    ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT
    GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT
    TGGAAGGCAT CTGCTAACAG AAAGCAAGTG GAAAACATTG CAAAGAAGAA ACTTGACTCG
    CTGTTGAAAG AATCAAAAAT CCGGGACCGC GAAGATCCTA ACAATTTCAC AGTCACCACA
    CTGCCACCAT CTGGGAAACT CAGCCAGAAA CCAGAATTAA AAAAGAAATG CAGCTCTGGA
    TCTAAAGGGG AAAGGGCTTT GAGTAAAACA GAGGAAGAAG CTGAGTCTGG AGAAGAGCCG
    AGCCCCAACA AAAGACACAG TCGTTCTTTG ATAGGAAGTT TTTCCAAGCA CAAGAAAAAA
    GGTGGAAAAA TAAAAAAATG CTCAAGCTTA GAAGAAGGGG ACGAAAGCCT GGACCGCCAG
    GGAAGTTTAG CAAGAGCTTC AGTACATTAT AGCAGAATAA ATAGACAAAA GAAAACCATG
    AAGCTGTCAA GAGCCCTGTC TGACCTGGTA AAATACACCA AATCAGTTGG TATCCATGAT
    GTGGAAACTG AAATCTCTTG CAGCTGGCAA GTTTCATCAT TTAGTGAAAC AAAAGCCCAC
    CAGATCCTTC AGCAAAAACC AGCTCAGTTT GTCCGGTTTA ACCAACACCA GCTCTCTCGC
    ATTTATCCAT CGTCTTACAG AGTCGACTCC AGCAACTACA ACCCACAACC ATTTTGGAAT
    GCTGGTTGTC AATTAGTTGC TTTAAACTAC CAGTCTGAAG GAAGAATGTT GCAGTTAAAC
    AGAGCCAAGT TCAACAGCAA TGGAAACTGC GGTTATGTGC TGAAACCGAA ATGCATGTGT
    CAAGGTGCCT TTAATCCTAA TTCTGAAGAT CCTTTGCCAG GACAATTGAA GAAACAGCTG
    GTTTTGCGAA TAATCAGTGG CCAACAGCTT CCAAAGCCAA AAGACTCCAT GCTGGGAGAT
    CGTGGAGAGA TCATTGATCC TTTTGTAGAA GTTGAGCTTA TTGGTTTGCC AGTGGATTGC
    TTTAAAGAAC AAACACGAGT TGTTGATGAT AATGGATTCA ATCCTATGTG GGAGGAAACC
    CTTGTATTTA CAGTGCACAT GCCAGAAATA TCATTAATTC GCTTCCTGGT GTGGGATCAT
    GATCCGATTG GCCGGGATTT CATTGGACAA CGGACTCTTT CATTTAGCAG CATGATGCCA
    GGCTATCGAC ATGTGTACCT GGAAGGTATG GAGGAGGCAT CCATCTTTGT TCATGTAGCA
    ATCAATGACA TCAGTGGTAA GGTCAAACAG GCCGCGGGCT TAAAAGGACT CTTTCTGAGA
    AATCCAAAGC AAGCTTCTCT GGACAGTCAT GCTGCTGGAC AGCTCCATCG GAAACATTCC
    ATTAGTAGTC ACATTTTAAG GAGAACTGCA AGTGCTCCTA CAAAGGGCCA ACAAAAAAAC
    AAAAAGGGCT TCCCAGAAAT TGCCATTGAC TCCAAAGAAA ACAGCTCGGA TGGACCTAGT
    GAAGACAGGG AGACTGATGA TTCGTCTCTG CCCCGCTTTG AAAAAGATCC TGAAAGTGCT
    TCTCCAGCTC TTCCTTCTAG GGATGGAACT AATGTGGAAT TAAACAGCAA GGGTTTAAAA
    GACCCTCGAC AATTATCAAT TTATAGATCG GCCTCCTCTT TATGCAGTCT TGAAACCATT
    AAAGAAGAGC CTCTCTTTGC CAAGGAAAAA CCTTCCACTA GTCCCCAGCC TGTTGCCTGT
    TTTACAAGTT GTGAAAGCAC AGCATGTGAT CAGTACAAGG AATCTCAGTC TGAATGCAGT
    AGCCCAGCTA TTACCTCTGA GAATGTACAA CGACCAAGTA AACTGAATGT TGAGCCAGCC
    ATAATCAAGG TGCTTGCTAT AAGTAATGGA CTACATCCAC ATAATGGAAA ACTTAATCCA
    GGAAAAGCAT ACAATGACAG GAATGGAAAA GTACTCAGTT CTGTCAGTGG GAAAAGTGAT
    TGCACAAATC TAATTAATTC ATCCCTGATT AAGCCTGGGT TGTCTTTACT TGCTGATGTT
    TCAAGAAATG TCAATGAACA ATATCAAGTT AAAGCTAACC AAGCTGCACA ATCTTCCATT
    CATGCTAGTA ATACTTCAGT TGCAAACAGA ATAATGTGCA CTAATCTACC TGATCATCAA
    ACTAGTTCAA AACCAAACAT GAAACATCTG ATTGTTCCTC CTGTTCAAAG ATCACAGTAT
    GACAGGCCTG ACTCAAAACC TTCTGTGGTT CATGCTGGCA AACACACACC TGTAAGGAGA
    GCAAAGAGTG AGGGCTATGT TGTTTCAGAC TGCTCAGCCT TAAGCTTAAA TTCTACATGT
    CAGTCCCCTG TCGCTGAAGT ATATTCTGAT GCTACAGTTA ATGATAGAAT TTGGAGCAAA
    CTGGAGTTAA ACTCTCATAG GGACAGTATG TCTTCGTCTT CCAGCAAGTC ATCCAATGAC
    ACAGTCATTG ATCTGTCTTT GCCTAATTTA GCCCGGAAAA GCTTATTGGA TCTTGACATC
    TCTGATGATA ACTTTGAAAA TATACATTTA AACAATACTA TTCGGCCAAG ATCCGCTACA
    TCTTCAACAA AGGAAATGTC GCTAGGAGTT TCAAAAAGTA AGTCTAACCC TAATTTAGTA
    TCCAATAACC AAGCAATAGA TGAACTCTCT TCTAGACCAC TGGCTAGAAG TCCTACAGAA
    GCATTGCCAC ACTTGCCACG AAGGAACACC TGGAATAGAT TATACGTAGA TAGTTTAAAA
    CAGGCAAATA AAAAGCCCAG TTTTGAGAAT AAACCACCAA CTAACCAGTC AATGTCGAAA
    AGTCTTGGAG ATCTGACTTC AGAAGATATT TCATGTAATT TTGAAAGTAA GTACAGAAGT
    ATAAGCAGAA GTTTTATTAC TAGATCAATG AGGGCACAAA GAAGACCGGC CAGTATGAAG
    GGTTCAGAGA AGTCTTCCGA TTCACTGACC GAACAGCTGA AAAAGCTAAC AAGCTTTCAG
    AAAGAAAATG ATATTACATC CCCAACTGAT TTTGAACCTG TTCATTGTAA CAATGATGAC
    ACAGCCTTCC TTCGAAGGAC GTCCTCCAGA AGTCAGAGTA GAGTAAGATA TATTGCAAAC
    CGAGCCAAGC AGGCTCAAGA AAGGCAAAGG CTCCAAAACC TGTGCCGGAT AAATGGAAAT
    TCCATAGAAG AAAGAGGGAA TCCAGAAGGG GCATGTGCAA TAACTAAAGG AATCTGTATG
    GATTCTCTTT CTCCCACACA GTTTACATCT GAAACATTGG ACAAATTTGG ACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61845
    Clone ID Related Accession (Same CDS sequence) XM_031905491.1
    Accession Version XM_031905491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4731bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
    GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC
    ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG
    GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG
    AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT
    GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC
    TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA
    TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG
    CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA
    GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC
    CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG
    CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC
    CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA
    TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT
    GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC
    ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT
    CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC
    ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC
    GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT
    CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA
    TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG
    GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC
    TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA
    ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT
    GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT
    GCATCTGCTA ACAGAAAGCA AGTGGAAAAC ATTGCAAAGA AGAAACTTGA CTCGCTGTTG
    AAAGAATCAA AAATCCGGGA CCGCGAAGAT CCTAACAATT TCACAGTCAC CACACTGCCA
    CCATCTGGGA AACTCAGCCA GAAACCAGAA TTAAAAAAGA AATGCAGCTC TGGATCTAAA
    GGGGAAAGGG CTTTGAGTAA AACAGAGGAA GAAGCTGAGT CTGGAGAAGA GCCGAGCCCC
    AACAAAAGAC ACAGTCGTTC TTTGATAGGA AGTTTTTCCA AGCACAAGAA AAAAGGTGGA
    AAAATAAAAA AATGCTCAAG CTTAGAAGAA GGGGACGAAA GCCTGGACCG CCAGGGAAGT
    TTAGCAAGAG CTTCAGTACA TTATAGCAGA ATAAATAGAC AAAAGAAAAC CATGAAGCTG
    TCAAGAGCCC TGTCTGACCT GGTAAAATAC ACCAAATCAG TTGGTATCCA TGATGTGGAA
    ACTGAAATCT CTTGCAGCTG GCAAGTTTCA TCATTTAGTG AAACAAAAGC CCACCAGATC
    CTTCAGCAAA AACCAGCTCA GTTTGTCCGG TTTAACCAAC ACCAGCTCTC TCGCATTTAT
    CCATCGTCTT ACAGAGTCGA CTCCAGCAAC TACAACCCAC AACCATTTTG GAATGCTGGT
    TGTCAATTAG TTGCTTTAAA CTACCAGTCT GAAGGAAGAA TGTTGCAGTT AAACAGAGCC
    AAGTTCAACA GCAATGGAAA CTGCGGTTAT GTGCTGAAAC CGAAATGCAT GTGTCAAGGT
    GCCTTTAATC CTAATTCTGA AGATCCTTTG CCAGGACAAT TGAAGAAACA GCTGGTTTTG
    CGAATAATCA GTGGCCAACA GCTTCCAAAG CCAAAAGACT CCATGCTGGG AGATCGTGGA
    GAGATCATTG ATCCTTTTGT AGAAGTTGAG CTTATTGGTT TGCCAGTGGA TTGCTTTAAA
    GAACAAACAC GAGTTGTTGA TGATAATGGA TTCAATCCTA TGTGGGAGGA AACCCTTGTA
    TTTACAGTGC ACATGCCAGA AATATCATTA ATTCGCTTCC TGGTGTGGGA TCATGATCCG
    ATTGGCCGGG ATTTCATTGG ACAACGGACT CTTTCATTTA GCAGCATGAT GCCAGGCTAT
    CGACATGTGT ACCTGGAAGG TATGGAGGAG GCATCCATCT TTGTTCATGT AGCAATCAAT
    GACATCAGTG GTAAGGTCAA ACAGGCCGCG GGCTTAAAAG GACTCTTTCT GAGAAATCCA
    AAGCAAGCTT CTCTGGACAG TCATGCTGCT GGACAGCTCC ATCGGAAACA TTCCATTAGT
    AGTCACATTT TAAGGAGAAC TGCAAGTGCT CCTACAAAGG GCCAACAAAA AAACAAAAAG
    GGCTTCCCAG AAATTGCCAT TGACTCCAAA GAAAACAGCT CGGATGGACC TAGTGAAGAC
    AGGGAGACTG ATGATTCGTC TCTGCCCCGC TTTGAAAAAG ATCCTGAAAG TGCTTCTCCA
    GCTCTTCCTT CTAGGGATGG AACTAATGTG GAATTAAACA GCAAGGGTTT AAAAGACCCT
    CGACAATTAT CAATTTATAG ATCGGCCTCC TCTTTATGCA GTCTTGAAAC CATTAAAGAA
    GAGCCTCTCT TTGCCAAGGA AAAACCTTCC ACTAGTCCCC AGCCTGTTGC CTGTTTTACA
    AGTTGTGAAA GCACAGCATG TGATCAGTAC AAGGAATCTC AGTCTGAATG CAGTAGCCCA
    GCTATTACCT CTGAGAATGT ACAACGACCA AGTAAACTGA ATGTTGAGCC AGCCATAATC
    AAGGTGCTTG CTATAAGTAA TGGACTACAT CCACATAATG GAAAACTTAA TCCAGGAAAA
    GCATACAATG ACAGGAATGG AAAAGTACTC AGTTCTGTCA GTGGGAAAAG TGATTGCACA
    AATCTAATTA ATTCATCCCT GATTAAGCCT GGGTTGTCTT TACTTGCTGA TGTTTCAAGA
    AATGTCAATG AACAATATCA AGTTAAAGCT AACCAAGCTG CACAATCTTC CATTCATGCT
    AGTAATACTT CAGTTGCAAA CAGAATAATG TGCACTAATC TACCTGATCA TCAAACTAGT
    TCAAAACCAA ACATGAAACA TCTGATTGTT CCTCCTGTTC AAAGATCACA GTATGACAGG
    CCTGACTCAA AACCTTCTGT GGTTCATGCT GGCAAACACA CACCTGTAAG GAGAGCAAAG
    AGTGAGGGCT ATGTTGTTTC AGACTGCTCA GCCTTAAGCT TAAATTCTAC ATGTCAGTCC
    CCTGTCGCTG AAGTATATTC TGATGCTACA GTTAATGATA GAATTTGGAG CAAACTGGAG
    TTAAACTCTC ATAGGGACAG TATGTCTTCG TCTTCCAGCA AGTCATCCAA TGACACAGTC
    ATTGATCTGT CTTTGCCTAA TTTAGCCCGG AAAAGCTTAT TGGATCTTGA CATCTCTGAT
    GATAACTTTG AAAATATACA TTTAAACAAT ACTATTCGGC CAAGATCCGC TACATCTTCA
    ACAAAGGAAA TGTCGCTAGG AGTTTCAAAA AGTAAGTCTA ACCCTAATTT AGTATCCAAT
    AACCAAGCAA TAGATGAACT CTCTTCTAGA CCACTGGCTA GAAGTCCTAC AGAAGCATTG
    CCACACTTGC CACGAAGGAA CACCTGGAAT AGATTATACG TAGATAGTTT AAAACAGGCA
    AATAAAAAGC CCAGTTTTGA GAATAAACCA CCAACTAACC AGTCAATGTC GAAAAGTCTT
    GGAGATCTGA CTTCAGAAGA TATTTCATGT AATTTTGAAA GTAAGTACAG AAGTATAAGC
    AGAAGTTTTA TTACTAGATC AATGAGGGCA CAAAGAAGAC CGGCCAGTAT GAAGGGTTCA
    GAGAAGTCTT CCGATTCACT GACCGAACAG CTGAAAAAGC TAACAAGCTT TCAGAAAGAA
    AATGATATTA CATCCCCAAC TGATTTTGAA CCTGTTCATT GTAACAATGA TGACACAGCC
    TTCCTTCGAA GGACGTCCTC CAGAAGTCAG AGTAGAGTAA GATATATTGC AAACCGAGCC
    AAGCAGGCTC AAGAAAGGCA AAGGCTCCAA AACCTGTGCC GGATAAATGG AAATTCCATA
    GAAGAAAGAG GGAATCCAGA AGGGGCATGT GCAATAACTA AAGGAATCTG TATGGATTCT
    CTTTCTCCCA CACAGTTTAC ATCTGAAACA TTGGACAAAT TTGGACCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761351.1
    CDS524..5254
    Translation

    Target ORF information:

    RefSeq Version XM_031905491.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCACTAT CATCTCCAAA AGTGGACATT AAAAAGAAGC CACTTGAGAC TTCTGTGAAT 
    GGCAAAGAAC AGGATTTTGT TTTTCTATCA GAAAATGTTG TGCTTGTGGT GGAAAGATGC
    ATGAGTTCTA TGCAAGCTGG TACCCAAATG ATCAAACTCC GGGGTAGCTC CAAAGGTTTG
    GTGCGTTTTT ATTACCTTGA CGAGCACAAG TCCTGTATAA GGTGGAGACC CTCAAGAAAG
    AATGAAAAGG CCAAGATCTC CATTGATTCC ATTCAGGAGG TGTGTGAGGG GAAACAGTCT
    GAGATTTTCC AGCGTTATGC AGATGGAAGC TTTGATCCAA ACTGCTGTTT CAGTATCTAC
    TATGGGGATC ACATGGAGTC TTTGGACTTG GTGTCCTCGA GTGGCGAAGA GGCTCGCACA
    TGGATTACTG GCCTGAAATA TCTCATGGCA GGGATAAGTG ACGAAGACAG TCTTTCCAAG
    CGTCAAAGAA CACGAGACCA ATGGTTGAAG CAGACATTTG ACGAGGCAGA CAAGAATGGA
    GACGGGTGCT TGAGTATTAA TGAGGTCTTA CAGCTCATGC ACAAACTGAA TGTCAACCTC
    CCACGACAGA AAGTGAAGCA AATGTTCAAG GAGGCAGACA CTGACGATAA CCAAGGCACG
    CTTGGTTTTG ATGAGTTCTG TGCATTTTAT AAGATGATGT CAACAAGAAG GGACCTATAC
    CTGCTAATGC TTACATACAG CAACCACAAG GACCACTTGG ATCCAGATGA CCTAAAACGA
    TTCCTAGAAG TTGAACAAAA GATGATAAAT GTGACAAAGG AATACTGCTT GGATATTGTT
    GATAAATTTG AACCATGCCC GGAGAATAAA AAGCAAGGGG CACTTGGAAT AGATGGTTTC
    ACTAACTATA TGCGCAGTCC GGCGGGTGAT ATCTTCAATC CAGACCACTA TCAAGTGAAT
    CAGGATATGA CCTTACCCTT GAGCAACTAT TTCATTGCAT CCTCTCACAA CACATATCTC
    ATGGGAGATC AGTTAATGTC TCAGTCTCGA GTAGACATGT ACGCGTGGGT ATTGCAAGCC
    GGTTGTCGTT GTGTTGAGGT GGACTGTTGG GACGGACCTG ATGGAGAACC AATAGTTCAT
    CATGGCTATA CTTTAACCTC TAAAATTCTT TTCAAAGATG TGATAGAAAC AATTAACAAA
    TATGCCTTCA TCAGAAATGA CTACCCTGTC ATCTTGTCTA TTGAAAATCA CTGCAGTGTG
    GTACAGCAGA AGAAGATGGC TCAGTACCTG ATTGAAATCT TGGGCGAGAA ATTAAATCTC
    TCTGATGTAG TCAATGAAGA TTCAACAAAA CTGCCGTCAC CGGAAAGCTT AAGGGGAAAA
    ATATTAGTGA AGGGAAAGAA GCTTCCTGCC AGTATCAGCG ATGACGCAGA GGAAGGAGAT
    GTTTCAGATG AAGACAGTGC AGATGAGATT GATGATGACT GCAAGTTAAT AAATGGCGAT
    GCATCTGCTA ACAGAAAGCA AGTGGAAAAC ATTGCAAAGA AGAAACTTGA CTCGCTGTTG
    AAAGAATCAA AAATCCGGGA CCGCGAAGAT CCTAACAATT TCACAGTCAC CACACTGCCA
    CCATCTGGGA AACTCAGCCA GAAACCAGAA TTAAAAAAGA AATGCAGCTC TGGATCTAAA
    GGGGAAAGGG CTTTGAGTAA AACAGAGGAA GAAGCTGAGT CTGGAGAAGA GCCGAGCCCC
    AACAAAAGAC ACAGTCGTTC TTTGATAGGA AGTTTTTCCA AGCACAAGAA AAAAGGTGGA
    AAAATAAAAA AATGCTCAAG CTTAGAAGAA GGGGACGAAA GCCTGGACCG CCAGGGAAGT
    TTAGCAAGAG CTTCAGTACA TTATAGCAGA ATAAATAGAC AAAAGAAAAC CATGAAGCTG
    TCAAGAGCCC TGTCTGACCT GGTAAAATAC ACCAAATCAG TTGGTATCCA TGATGTGGAA
    ACTGAAATCT CTTGCAGCTG GCAAGTTTCA TCATTTAGTG AAACAAAAGC CCACCAGATC
    CTTCAGCAAA AACCAGCTCA GTTTGTCCGG TTTAACCAAC ACCAGCTCTC TCGCATTTAT
    CCATCGTCTT ACAGAGTCGA CTCCAGCAAC TACAACCCAC AACCATTTTG GAATGCTGGT
    TGTCAATTAG TTGCTTTAAA CTACCAGTCT GAAGGAAGAA TGTTGCAGTT AAACAGAGCC
    AAGTTCAACA GCAATGGAAA CTGCGGTTAT GTGCTGAAAC CGAAATGCAT GTGTCAAGGT
    GCCTTTAATC CTAATTCTGA AGATCCTTTG CCAGGACAAT TGAAGAAACA GCTGGTTTTG
    CGAATAATCA GTGGCCAACA GCTTCCAAAG CCAAAAGACT CCATGCTGGG AGATCGTGGA
    GAGATCATTG ATCCTTTTGT AGAAGTTGAG CTTATTGGTT TGCCAGTGGA TTGCTTTAAA
    GAACAAACAC GAGTTGTTGA TGATAATGGA TTCAATCCTA TGTGGGAGGA AACCCTTGTA
    TTTACAGTGC ACATGCCAGA AATATCATTA ATTCGCTTCC TGGTGTGGGA TCATGATCCG
    ATTGGCCGGG ATTTCATTGG ACAACGGACT CTTTCATTTA GCAGCATGAT GCCAGGCTAT
    CGACATGTGT ACCTGGAAGG TATGGAGGAG GCATCCATCT TTGTTCATGT AGCAATCAAT
    GACATCAGTG GTAAGGTCAA ACAGGCCGCG GGCTTAAAAG GACTCTTTCT GAGAAATCCA
    AAGCAAGCTT CTCTGGACAG TCATGCTGCT GGACAGCTCC ATCGGAAACA TTCCATTAGT
    AGTCACATTT TAAGGAGAAC TGCAAGTGCT CCTACAAAGG GCCAACAAAA AAACAAAAAG
    GGCTTCCCAG AAATTGCCAT TGACTCCAAA GAAAACAGCT CGGATGGACC TAGTGAAGAC
    AGGGAGACTG ATGATTCGTC TCTGCCCCGC TTTGAAAAAG ATCCTGAAAG TGCTTCTCCA
    GCTCTTCCTT CTAGGGATGG AACTAATGTG GAATTAAACA GCAAGGGTTT AAAAGACCCT
    CGACAATTAT CAATTTATAG ATCGGCCTCC TCTTTATGCA GTCTTGAAAC CATTAAAGAA
    GAGCCTCTCT TTGCCAAGGA AAAACCTTCC ACTAGTCCCC AGCCTGTTGC CTGTTTTACA
    AGTTGTGAAA GCACAGCATG TGATCAGTAC AAGGAATCTC AGTCTGAATG CAGTAGCCCA
    GCTATTACCT CTGAGAATGT ACAACGACCA AGTAAACTGA ATGTTGAGCC AGCCATAATC
    AAGGTGCTTG CTATAAGTAA TGGACTACAT CCACATAATG GAAAACTTAA TCCAGGAAAA
    GCATACAATG ACAGGAATGG AAAAGTACTC AGTTCTGTCA GTGGGAAAAG TGATTGCACA
    AATCTAATTA ATTCATCCCT GATTAAGCCT GGGTTGTCTT TACTTGCTGA TGTTTCAAGA
    AATGTCAATG AACAATATCA AGTTAAAGCT AACCAAGCTG CACAATCTTC CATTCATGCT
    AGTAATACTT CAGTTGCAAA CAGAATAATG TGCACTAATC TACCTGATCA TCAAACTAGT
    TCAAAACCAA ACATGAAACA TCTGATTGTT CCTCCTGTTC AAAGATCACA GTATGACAGG
    CCTGACTCAA AACCTTCTGT GGTTCATGCT GGCAAACACA CACCTGTAAG GAGAGCAAAG
    AGTGAGGGCT ATGTTGTTTC AGACTGCTCA GCCTTAAGCT TAAATTCTAC ATGTCAGTCC
    CCTGTCGCTG AAGTATATTC TGATGCTACA GTTAATGATA GAATTTGGAG CAAACTGGAG
    TTAAACTCTC ATAGGGACAG TATGTCTTCG TCTTCCAGCA AGTCATCCAA TGACACAGTC
    ATTGATCTGT CTTTGCCTAA TTTAGCCCGG AAAAGCTTAT TGGATCTTGA CATCTCTGAT
    GATAACTTTG AAAATATACA TTTAAACAAT ACTATTCGGC CAAGATCCGC TACATCTTCA
    ACAAAGGAAA TGTCGCTAGG AGTTTCAAAA AGTAAGTCTA ACCCTAATTT AGTATCCAAT
    AACCAAGCAA TAGATGAACT CTCTTCTAGA CCACTGGCTA GAAGTCCTAC AGAAGCATTG
    CCACACTTGC CACGAAGGAA CACCTGGAAT AGATTATACG TAGATAGTTT AAAACAGGCA
    AATAAAAAGC CCAGTTTTGA GAATAAACCA CCAACTAACC AGTCAATGTC GAAAAGTCTT
    GGAGATCTGA CTTCAGAAGA TATTTCATGT AATTTTGAAA GTAAGTACAG AAGTATAAGC
    AGAAGTTTTA TTACTAGATC AATGAGGGCA CAAAGAAGAC CGGCCAGTAT GAAGGGTTCA
    GAGAAGTCTT CCGATTCACT GACCGAACAG CTGAAAAAGC TAACAAGCTT TCAGAAAGAA
    AATGATATTA CATCCCCAAC TGATTTTGAA CCTGTTCATT GTAACAATGA TGACACAGCC
    TTCCTTCGAA GGACGTCCTC CAGAAGTCAG AGTAGAGTAA GATATATTGC AAACCGAGCC
    AAGCAGGCTC AAGAAAGGCA AAGGCTCCAA AACCTGTGCC GGATAAATGG AAATTCCATA
    GAAGAAAGAG GGAATCCAGA AGGGGCATGT GCAATAACTA AAGGAATCTG TATGGATTCT
    CTTTCTCCCA CACAGTTTAC ATCTGAAACA TTGGACAAAT TTGGACCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61846
    Clone ID Related Accession (Same CDS sequence) XM_018096045.2
    Accession Version XM_018096045.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4683bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096045.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
    AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA
    GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA
    AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA
    CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT
    ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT
    CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT
    TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG
    AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC
    AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA
    GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC
    CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA
    AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT
    ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT
    GGTTTCACTA ACTATATGCG CAGTCCGGCG GGTGATATCT TCAATCCAGA CCACTATCAA
    GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA
    TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG
    CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA
    GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT
    AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC
    AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA
    AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG
    GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA
    GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT
    GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT
    GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC
    ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC
    TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA
    GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG
    AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC
    CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA
    ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC
    CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA
    GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC
    TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT
    TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG
    TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC
    ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA
    CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG
    GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG
    GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG
    GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG
    GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG
    ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT
    GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT
    CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA
    CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA
    AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA
    CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA
    AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT
    TTAAAAGACC CTCGACAATT ATCAATTTAT AGATCGGCCT CCTCTTTATG CAGTCTTGAA
    ACCATTAAAG AAGAGCCTCT CTTTGCCAAG GAAAAACCTT CCACTAGTCC CCAGCCTGTT
    GCCTGTTTTA CAAGTTGTGA AAGCACAGCA TGTGATCAGT ACAAGGAATC TCAGTCTGAA
    TGCAGTAGCC CAGCTATTAC CTCTGAGAAT GTACAACGAC CAAGTAAACT GAATGTTGAG
    CCAGCCATAA TCAAGGTGCT TGCTATAAGT AATGGACTAC ATCCACATAA TGGAAAACTT
    AATCCAGGAA AAGCATACAA TGACAGGAAT GGAAAAGTAC TCAGTTCTGT CAGTGGGAAA
    AGTGATTGCA CAAATCTAAT TAATTCATCC CTGATTAAGC CTGGGTTGTC TTTACTTGCT
    GATGTTTCAA GAAATGTCAA TGAACAATAT CAAGTTAAAG CTAACCAAGC TGCACAATCT
    TCCATTCATG CTAGTAATAC TTCAGTTGCA AACAGAATAA TGTGCACTAA TCTACCTGAT
    CATCAAACTA GTTCAAAACC AAACATGAAA CATCTGATTG TTCCTCCTGT TCAAAGATCA
    CAGTATGACA GGCCTGACTC AAAACCTTCT GTGGTTCATG CTGGCAAACA CACACCTGTA
    AGGAGAGCAA AGAGTGAGGG CTATGTTGTT TCAGACTGCT CAGCCTTAAG CTTAAATTCT
    ACATGTCAGT CCCCTGTCGC TGAAGTATAT TCTGATGCTA CAGTTAATGA TAGAATTTGG
    AGCAAACTGG AGTTAAACTC TCATAGGGAC AGTATGTCTT CGTCTTCCAG CAAGTCATCC
    AATGACACAG TCATTGATCT GTCTTTGCCT AATTTAGCCC GGAAAAGCTT ATTGGATCTT
    GACATCTCTG ATGATAACTT TGAAAATATA CATTTAAACA ATACTATTCG GCCAAGATCC
    GCTACATCTT CAACAAAGGA AATGTCGCTA GGAGTTTCAA AAAGTAAGTC TAACCCTAAT
    TTAGTATCCA ATAACCAAGC AATAGATGAA CTCTCTTCTA GACCACTGGC TAGAAGTCCT
    ACAGAAGCAT TGCCACACTT GCCACGAAGG AACACCTGGA ATAGATTATA CGTAGATAGT
    TTAAAACAGG CAAATAAAAA GCCCAGTTTT GAGAATAAAC CACCAACTAA CCAGTCAATG
    TCGAAAAGTC TTGGAGATCT GACTTCAGAA GATATTTCAT GTAATTTTGA AAGTAAGTAC
    AGAAGTATAA GCAGAAGTTT TATTACTAGA TCAATGAGGG CACAAAGAAG ACCGGCCAGT
    ATGAAGGGTT CAGAGAAGTC TTCCGATTCA CTGACCGAAC AGCTGAAAAA GCTAACAAGC
    TTTCAGAAAG AAAATGATAT TACATCCCCA ACTGATTTTG AACCTGTTCA TTGTAACAAT
    GATGACACAG CCTTCCTTCG AAGGACGTCC TCCAGAAGTC AGAGTAGAGT AAGATATATT
    GCAAACCGAG CCAAGCAGGC TCAAGAAAGG CAAAGGCTCC AAAACCTGTG CCGGATAAAT
    GGAAATTCCA TAGAAGAAAG AGGGAATCCA GAAGGGGCAT GTGCAATAAC TAAAGGAATC
    TGTATGGATT CTCTTTCTCC CACACAGTTT ACATCTGAAA CATTGGACAA ATTTGGACCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017951534.2
    CDS88..4770
    Translation

    Target ORF information:

    RefSeq Version XM_018096045.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096045.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGTCCGTT ATTCCTGGCA CTTTTGGCGC TTGAAAAAAC GCCTCAATTA CCAAGTGGAA 
    AGATGCATGA GTTCTATGCA AGCTGGTACC CAAATGATCA AACTCCGGGG TAGCTCCAAA
    GGTTTGGTGC GTTTTTATTA CCTTGACGAG CACAAGTCCT GTATAAGGTG GAGACCCTCA
    AGAAAGAATG AAAAGGCCAA GATCTCCATT GATTCCATTC AGGAGGTGTG TGAGGGGAAA
    CAGTCTGAGA TTTTCCAGCG TTATGCAGAT GGAAGCTTTG ATCCAAACTG CTGTTTCAGT
    ATCTACTATG GGGATCACAT GGAGTCTTTG GACTTGGTGT CCTCGAGTGG CGAAGAGGCT
    CGCACATGGA TTACTGGCCT GAAATATCTC ATGGCAGGGA TAAGTGACGA AGACAGTCTT
    TCCAAGCGTC AAAGAACACG AGACCAATGG TTGAAGCAGA CATTTGACGA GGCAGACAAG
    AATGGAGACG GGTGCTTGAG TATTAATGAG GTCTTACAGC TCATGCACAA ACTGAATGTC
    AACCTCCCAC GACAGAAAGT GAAGCAAATG TTCAAGGAGG CAGACACTGA CGATAACCAA
    GGCACGCTTG GTTTTGATGA GTTCTGTGCA TTTTATAAGA TGATGTCAAC AAGAAGGGAC
    CTATACCTGC TAATGCTTAC ATACAGCAAC CACAAGGACC ACTTGGATCC AGATGACCTA
    AAACGATTCC TAGAAGTTGA ACAAAAGATG ATAAATGTGA CAAAGGAATA CTGCTTGGAT
    ATTGTTGATA AATTTGAACC ATGCCCGGAG AATAAAAAGC AAGGGGCACT TGGAATAGAT
    GGTTTCACTA ACTATATGCG CAGTCCGGCG GGTGATATCT TCAATCCAGA CCACTATCAA
    GTGAATCAGG ATATGACCTT ACCCTTGAGC AACTATTTCA TTGCATCCTC TCACAACACA
    TATCTCATGG GAGATCAGTT AATGTCTCAG TCTCGAGTAG ACATGTACGC GTGGGTATTG
    CAAGCCGGTT GTCGTTGTGT TGAGGTGGAC TGTTGGGACG GACCTGATGG AGAACCAATA
    GTTCATCATG GCTATACTTT AACCTCTAAA ATTCTTTTCA AAGATGTGAT AGAAACAATT
    AACAAATATG CCTTCATCAG AAATGACTAC CCTGTCATCT TGTCTATTGA AAATCACTGC
    AGTGTGGTAC AGCAGAAGAA GATGGCTCAG TACCTGATTG AAATCTTGGG CGAGAAATTA
    AATCTCTCTG ATGTAGTCAA TGAAGATTCA ACAAAACTGC CGTCACCGGA AAGCTTAAGG
    GGAAAAATAT TAGTGAAGGG AAAGAAGCTT CCTGCCAGTA TCAGCGATGA CGCAGAGGAA
    GGAGATGTTT CAGATGAAGA CAGTGCAGAT GAGATTGATG ATGACTGCAA GTTAATAAAT
    GGCGATTGGA AGGCATCTGC TAACAGAAAG CAAGTGGAAA ACATTGCAAA GAAGAAACTT
    GACTCGCTGT TGAAAGAATC AAAAATCCGG GACCGCGAAG ATCCTAACAA TTTCACAGTC
    ACCACACTGC CACCATCTGG GAAACTCAGC CAGAAACCAG AATTAAAAAA GAAATGCAGC
    TCTGGATCTA AAGGGGAAAG GGCTTTGAGT AAAACAGAGG AAGAAGCTGA GTCTGGAGAA
    GAGCCGAGCC CCAACAAAAG ACACAGTCGT TCTTTGATAG GAAGTTTTTC CAAGCACAAG
    AAAAAAGGTG GAAAAATAAA AAAATGCTCA AGCTTAGAAG AAGGGGACGA AAGCCTGGAC
    CGCCAGGGAA GTTTAGCAAG AGCTTCAGTA CATTATAGCA GAATAAATAG ACAAAAGAAA
    ACCATGAAGC TGTCAAGAGC CCTGTCTGAC CTGGTAAAAT ACACCAAATC AGTTGGTATC
    CATGATGTGG AAACTGAAAT CTCTTGCAGC TGGCAAGTTT CATCATTTAG TGAAACAAAA
    GCCCACCAGA TCCTTCAGCA AAAACCAGCT CAGTTTGTCC GGTTTAACCA ACACCAGCTC
    TCTCGCATTT ATCCATCGTC TTACAGAGTC GACTCCAGCA ACTACAACCC ACAACCATTT
    TGGAATGCTG GTTGTCAATT AGTTGCTTTA AACTACCAGT CTGAAGGAAG AATGTTGCAG
    TTAAACAGAG CCAAGTTCAA CAGCAATGGA AACTGCGGTT ATGTGCTGAA ACCGAAATGC
    ATGTGTCAAG GTGCCTTTAA TCCTAATTCT GAAGATCCTT TGCCAGGACA ATTGAAGAAA
    CAGCTGGTTT TGCGAATAAT CAGTGGCCAA CAGCTTCCAA AGCCAAAAGA CTCCATGCTG
    GGAGATCGTG GAGAGATCAT TGATCCTTTT GTAGAAGTTG AGCTTATTGG TTTGCCAGTG
    GATTGCTTTA AAGAACAAAC ACGAGTTGTT GATGATAATG GATTCAATCC TATGTGGGAG
    GAAACCCTTG TATTTACAGT GCACATGCCA GAAATATCAT TAATTCGCTT CCTGGTGTGG
    GATCATGATC CGATTGGCCG GGATTTCATT GGACAACGGA CTCTTTCATT TAGCAGCATG
    ATGCCAGGCT ATCGACATGT GTACCTGGAA GGTATGGAGG AGGCATCCAT CTTTGTTCAT
    GTAGCAATCA ATGACATCAG TGGTAAGGTC AAACAGGCCG CGGGCTTAAA AGGACTCTTT
    CTGAGAAATC CAAAGCAAGC TTCTCTGGAC AGTCATGCTG CTGGACAGCT CCATCGGAAA
    CATTCCATTA GTAGTCACAT TTTAAGGAGA ACTGCAAGTG CTCCTACAAA GGGCCAACAA
    AAAAACAAAA AGGGCTTCCC AGAAATTGCC ATTGACTCCA AAGAAAACAG CTCGGATGGA
    CCTAGTGAAG ACAGGGAGAC TGATGATTCG TCTCTGCCCC GCTTTGAAAA AGATCCTGAA
    AGTGCTTCTC CAGCTCTTCC TTCTAGGGAT GGAACTAATG TGGAATTAAA CAGCAAGGGT
    TTAAAAGACC CTCGACAATT ATCAATTTAT AGATCGGCCT CCTCTTTATG CAGTCTTGAA
    ACCATTAAAG AAGAGCCTCT CTTTGCCAAG GAAAAACCTT CCACTAGTCC CCAGCCTGTT
    GCCTGTTTTA CAAGTTGTGA AAGCACAGCA TGTGATCAGT ACAAGGAATC TCAGTCTGAA
    TGCAGTAGCC CAGCTATTAC CTCTGAGAAT GTACAACGAC CAAGTAAACT GAATGTTGAG
    CCAGCCATAA TCAAGGTGCT TGCTATAAGT AATGGACTAC ATCCACATAA TGGAAAACTT
    AATCCAGGAA AAGCATACAA TGACAGGAAT GGAAAAGTAC TCAGTTCTGT CAGTGGGAAA
    AGTGATTGCA CAAATCTAAT TAATTCATCC CTGATTAAGC CTGGGTTGTC TTTACTTGCT
    GATGTTTCAA GAAATGTCAA TGAACAATAT CAAGTTAAAG CTAACCAAGC TGCACAATCT
    TCCATTCATG CTAGTAATAC TTCAGTTGCA AACAGAATAA TGTGCACTAA TCTACCTGAT
    CATCAAACTA GTTCAAAACC AAACATGAAA CATCTGATTG TTCCTCCTGT TCAAAGATCA
    CAGTATGACA GGCCTGACTC AAAACCTTCT GTGGTTCATG CTGGCAAACA CACACCTGTA
    AGGAGAGCAA AGAGTGAGGG CTATGTTGTT TCAGACTGCT CAGCCTTAAG CTTAAATTCT
    ACATGTCAGT CCCCTGTCGC TGAAGTATAT TCTGATGCTA CAGTTAATGA TAGAATTTGG
    AGCAAACTGG AGTTAAACTC TCATAGGGAC AGTATGTCTT CGTCTTCCAG CAAGTCATCC
    AATGACACAG TCATTGATCT GTCTTTGCCT AATTTAGCCC GGAAAAGCTT ATTGGATCTT
    GACATCTCTG ATGATAACTT TGAAAATATA CATTTAAACA ATACTATTCG GCCAAGATCC
    GCTACATCTT CAACAAAGGA AATGTCGCTA GGAGTTTCAA AAAGTAAGTC TAACCCTAAT
    TTAGTATCCA ATAACCAAGC AATAGATGAA CTCTCTTCTA GACCACTGGC TAGAAGTCCT
    ACAGAAGCAT TGCCACACTT GCCACGAAGG AACACCTGGA ATAGATTATA CGTAGATAGT
    TTAAAACAGG CAAATAAAAA GCCCAGTTTT GAGAATAAAC CACCAACTAA CCAGTCAATG
    TCGAAAAGTC TTGGAGATCT GACTTCAGAA GATATTTCAT GTAATTTTGA AAGTAAGTAC
    AGAAGTATAA GCAGAAGTTT TATTACTAGA TCAATGAGGG CACAAAGAAG ACCGGCCAGT
    ATGAAGGGTT CAGAGAAGTC TTCCGATTCA CTGACCGAAC AGCTGAAAAA GCTAACAAGC
    TTTCAGAAAG AAAATGATAT TACATCCCCA ACTGATTTTG AACCTGTTCA TTGTAACAAT
    GATGACACAG CCTTCCTTCG AAGGACGTCC TCCAGAAGTC AGAGTAGAGT AAGATATATT
    GCAAACCGAG CCAAGCAGGC TCAAGAAAGG CAAAGGCTCC AAAACCTGTG CCGGATAAAT
    GGAAATTCCA TAGAAGAAAG AGGGAATCCA GAAGGGGCAT GTGCAATAAC TAAAGGAATC
    TGTATGGATT CTCTTTCTCC CACACAGTTT ACATCTGAAA CATTGGACAA ATTTGGACCT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa61847
    Clone ID Related Accession (Same CDS sequence) XM_031905492.1
    Accession Version XM_031905492.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4659bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGATTTAG GAAGTCTTCC GCAGAAGCAC ATGGAAAGAT GCATGAGTTC TATGCAAGCT 
    GGTACCCAAA TGATCAAACT CCGGGGTAGC TCCAAAGGTT TGGTGCGTTT TTATTACCTT
    GACGAGCACA AGTCCTGTAT AAGGTGGAGA CCCTCAAGAA AGAATGAAAA GGCCAAGATC
    TCCATTGATT CCATTCAGGA GGTGTGTGAG GGGAAACAGT CTGAGATTTT CCAGCGTTAT
    GCAGATGGAA GCTTTGATCC AAACTGCTGT TTCAGTATCT ACTATGGGGA TCACATGGAG
    TCTTTGGACT TGGTGTCCTC GAGTGGCGAA GAGGCTCGCA CATGGATTAC TGGCCTGAAA
    TATCTCATGG CAGGGATAAG TGACGAAGAC AGTCTTTCCA AGCGTCAAAG AACACGAGAC
    CAATGGTTGA AGCAGACATT TGACGAGGCA GACAAGAATG GAGACGGGTG CTTGAGTATT
    AATGAGGTCT TACAGCTCAT GCACAAACTG AATGTCAACC TCCCACGACA GAAAGTGAAG
    CAAATGTTCA AGGAGGCAGA CACTGACGAT AACCAAGGCA CGCTTGGTTT TGATGAGTTC
    TGTGCATTTT ATAAGATGAT GTCAACAAGA AGGGACCTAT ACCTGCTAAT GCTTACATAC
    AGCAACCACA AGGACCACTT GGATCCAGAT GACCTAAAAC GATTCCTAGA AGTTGAACAA
    AAGATGATAA ATGTGACAAA GGAATACTGC TTGGATATTG TTGATAAATT TGAACCATGC
    CCGGAGAATA AAAAGCAAGG GGCACTTGGA ATAGATGGTT TCACTAACTA TATGCGCAGT
    CCGGCGGGTG ATATCTTCAA TCCAGACCAC TATCAAGTGA ATCAGGATAT GACCTTACCC
    TTGAGCAACT ATTTCATTGC ATCCTCTCAC AACACATATC TCATGGGAGA TCAGTTAATG
    TCTCAGTCTC GAGTAGACAT GTACGCGTGG GTATTGCAAG CCGGTTGTCG TTGTGTTGAG
    GTGGACTGTT GGGACGGACC TGATGGAGAA CCAATAGTTC ATCATGGCTA TACTTTAACC
    TCTAAAATTC TTTTCAAAGA TGTGATAGAA ACAATTAACA AATATGCCTT CATCAGAAAT
    GACTACCCTG TCATCTTGTC TATTGAAAAT CACTGCAGTG TGGTACAGCA GAAGAAGATG
    GCTCAGTACC TGATTGAAAT CTTGGGCGAG AAATTAAATC TCTCTGATGT AGTCAATGAA
    GATTCAACAA AACTGCCGTC ACCGGAAAGC TTAAGGGGAA AAATATTAGT GAAGGGAAAG
    AAGCTTCCTG CCAGTATCAG CGATGACGCA GAGGAAGGAG ATGTTTCAGA TGAAGACAGT
    GCAGATGAGA TTGATGATGA CTGCAAGTTA ATAAATGGCG ATTGGAAGGC ATCTGCTAAC
    AGAAAGCAAG TGGAAAACAT TGCAAAGAAG AAACTTGACT CGCTGTTGAA AGAATCAAAA
    ATCCGGGACC GCGAAGATCC TAACAATTTC ACAGTCACCA CACTGCCACC ATCTGGGAAA
    CTCAGCCAGA AACCAGAATT AAAAAAGAAA TGCAGCTCTG GATCTAAAGG GGAAAGGGCT
    TTGAGTAAAA CAGAGGAAGA AGCTGAGTCT GGAGAAGAGC CGAGCCCCAA CAAAAGACAC
    AGTCGTTCTT TGATAGGAAG TTTTTCCAAG CACAAGAAAA AAGGTGGAAA AATAAAAAAA
    TGCTCAAGCT TAGAAGAAGG GGACGAAAGC CTGGACCGCC AGGGAAGTTT AGCAAGAGCT
    TCAGTACATT ATAGCAGAAT AAATAGACAA AAGAAAACCA TGAAGCTGTC AAGAGCCCTG
    TCTGACCTGG TAAAATACAC CAAATCAGTT GGTATCCATG ATGTGGAAAC TGAAATCTCT
    TGCAGCTGGC AAGTTTCATC ATTTAGTGAA ACAAAAGCCC ACCAGATCCT TCAGCAAAAA
    CCAGCTCAGT TTGTCCGGTT TAACCAACAC CAGCTCTCTC GCATTTATCC ATCGTCTTAC
    AGAGTCGACT CCAGCAACTA CAACCCACAA CCATTTTGGA ATGCTGGTTG TCAATTAGTT
    GCTTTAAACT ACCAGTCTGA AGGAAGAATG TTGCAGTTAA ACAGAGCCAA GTTCAACAGC
    AATGGAAACT GCGGTTATGT GCTGAAACCG AAATGCATGT GTCAAGGTGC CTTTAATCCT
    AATTCTGAAG ATCCTTTGCC AGGACAATTG AAGAAACAGC TGGTTTTGCG AATAATCAGT
    GGCCAACAGC TTCCAAAGCC AAAAGACTCC ATGCTGGGAG ATCGTGGAGA GATCATTGAT
    CCTTTTGTAG AAGTTGAGCT TATTGGTTTG CCAGTGGATT GCTTTAAAGA ACAAACACGA
    GTTGTTGATG ATAATGGATT CAATCCTATG TGGGAGGAAA CCCTTGTATT TACAGTGCAC
    ATGCCAGAAA TATCATTAAT TCGCTTCCTG GTGTGGGATC ATGATCCGAT TGGCCGGGAT
    TTCATTGGAC AACGGACTCT TTCATTTAGC AGCATGATGC CAGGCTATCG ACATGTGTAC
    CTGGAAGGTA TGGAGGAGGC ATCCATCTTT GTTCATGTAG CAATCAATGA CATCAGTGGT
    AAGGTCAAAC AGGCCGCGGG CTTAAAAGGA CTCTTTCTGA GAAATCCAAA GCAAGCTTCT
    CTGGACAGTC ATGCTGCTGG ACAGCTCCAT CGGAAACATT CCATTAGTAG TCACATTTTA
    AGGAGAACTG CAAGTGCTCC TACAAAGGGC CAACAAAAAA ACAAAAAGGG CTTCCCAGAA
    ATTGCCATTG ACTCCAAAGA AAACAGCTCG GATGGACCTA GTGAAGACAG GGAGACTGAT
    GATTCGTCTC TGCCCCGCTT TGAAAAAGAT CCTGAAAGTG CTTCTCCAGC TCTTCCTTCT
    AGGGATGGAA CTAATGTGGA ATTAAACAGC AAGGGTTTAA AAGACCCTCG ACAATTATCA
    ATTTATAGAT CGGCCTCCTC TTTATGCAGT CTTGAAACCA TTAAAGAAGA GCCTCTCTTT
    GCCAAGGAAA AACCTTCCAC TAGTCCCCAG CCTGTTGCCT GTTTTACAAG TTGTGAAAGC
    ACAGCATGTG ATCAGTACAA GGAATCTCAG TCTGAATGCA GTAGCCCAGC TATTACCTCT
    GAGAATGTAC AACGACCAAG TAAACTGAAT GTTGAGCCAG CCATAATCAA GGTGCTTGCT
    ATAAGTAATG GACTACATCC ACATAATGGA AAACTTAATC CAGGAAAAGC ATACAATGAC
    AGGAATGGAA AAGTACTCAG TTCTGTCAGT GGGAAAAGTG ATTGCACAAA TCTAATTAAT
    TCATCCCTGA TTAAGCCTGG GTTGTCTTTA CTTGCTGATG TTTCAAGAAA TGTCAATGAA
    CAATATCAAG TTAAAGCTAA CCAAGCTGCA CAATCTTCCA TTCATGCTAG TAATACTTCA
    GTTGCAAACA GAATAATGTG CACTAATCTA CCTGATCATC AAACTAGTTC AAAACCAAAC
    ATGAAACATC TGATTGTTCC TCCTGTTCAA AGATCACAGT ATGACAGGCC TGACTCAAAA
    CCTTCTGTGG TTCATGCTGG CAAACACACA CCTGTAAGGA GAGCAAAGAG TGAGGGCTAT
    GTTGTTTCAG ACTGCTCAGC CTTAAGCTTA AATTCTACAT GTCAGTCCCC TGTCGCTGAA
    GTATATTCTG ATGCTACAGT TAATGATAGA ATTTGGAGCA AACTGGAGTT AAACTCTCAT
    AGGGACAGTA TGTCTTCGTC TTCCAGCAAG TCATCCAATG ACACAGTCAT TGATCTGTCT
    TTGCCTAATT TAGCCCGGAA AAGCTTATTG GATCTTGACA TCTCTGATGA TAACTTTGAA
    AATATACATT TAAACAATAC TATTCGGCCA AGATCCGCTA CATCTTCAAC AAAGGAAATG
    TCGCTAGGAG TTTCAAAAAG TAAGTCTAAC CCTAATTTAG TATCCAATAA CCAAGCAATA
    GATGAACTCT CTTCTAGACC ACTGGCTAGA AGTCCTACAG AAGCATTGCC ACACTTGCCA
    CGAAGGAACA CCTGGAATAG ATTATACGTA GATAGTTTAA AACAGGCAAA TAAAAAGCCC
    AGTTTTGAGA ATAAACCACC AACTAACCAG TCAATGTCGA AAAGTCTTGG AGATCTGACT
    TCAGAAGATA TTTCATGTAA TTTTGAAAGT AAGTACAGAA GTATAAGCAG AAGTTTTATT
    ACTAGATCAA TGAGGGCACA AAGAAGACCG GCCAGTATGA AGGGTTCAGA GAAGTCTTCC
    GATTCACTGA CCGAACAGCT GAAAAAGCTA ACAAGCTTTC AGAAAGAAAA TGATATTACA
    TCCCCAACTG ATTTTGAACC TGTTCATTGT AACAATGATG ACACAGCCTT CCTTCGAAGG
    ACGTCCTCCA GAAGTCAGAG TAGAGTAAGA TATATTGCAA ACCGAGCCAA GCAGGCTCAA
    GAAAGGCAAA GGCTCCAAAA CCTGTGCCGG ATAAATGGAA ATTCCATAGA AGAAAGAGGG
    AATCCAGAAG GGGCATGTGC AATAACTAAA GGAATCTGTA TGGATTCTCT TTCTCCCACA
    CAGTTTACAT CTGAAACATT GGACAAATTT GGACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031761352.1
    CDS242..4900
    Translation

    Target ORF information:

    RefSeq Version XM_031905492.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis phospholipase C eta 2 (plch2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031905492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGATTTAG GAAGTCTTCC GCAGAAGCAC ATGGAAAGAT GCATGAGTTC TATGCAAGCT 
    GGTACCCAAA TGATCAAACT CCGGGGTAGC TCCAAAGGTT TGGTGCGTTT TTATTACCTT
    GACGAGCACA AGTCCTGTAT AAGGTGGAGA CCCTCAAGAA AGAATGAAAA GGCCAAGATC
    TCCATTGATT CCATTCAGGA GGTGTGTGAG GGGAAACAGT CTGAGATTTT CCAGCGTTAT
    GCAGATGGAA GCTTTGATCC AAACTGCTGT TTCAGTATCT ACTATGGGGA TCACATGGAG
    TCTTTGGACT TGGTGTCCTC GAGTGGCGAA GAGGCTCGCA CATGGATTAC TGGCCTGAAA
    TATCTCATGG CAGGGATAAG TGACGAAGAC AGTCTTTCCA AGCGTCAAAG AACACGAGAC
    CAATGGTTGA AGCAGACATT TGACGAGGCA GACAAGAATG GAGACGGGTG CTTGAGTATT
    AATGAGGTCT TACAGCTCAT GCACAAACTG AATGTCAACC TCCCACGACA GAAAGTGAAG
    CAAATGTTCA AGGAGGCAGA CACTGACGAT AACCAAGGCA CGCTTGGTTT TGATGAGTTC
    TGTGCATTTT ATAAGATGAT GTCAACAAGA AGGGACCTAT ACCTGCTAAT GCTTACATAC
    AGCAACCACA AGGACCACTT GGATCCAGAT GACCTAAAAC GATTCCTAGA AGTTGAACAA
    AAGATGATAA ATGTGACAAA GGAATACTGC TTGGATATTG TTGATAAATT TGAACCATGC
    CCGGAGAATA AAAAGCAAGG GGCACTTGGA ATAGATGGTT TCACTAACTA TATGCGCAGT
    CCGGCGGGTG ATATCTTCAA TCCAGACCAC TATCAAGTGA ATCAGGATAT GACCTTACCC
    TTGAGCAACT ATTTCATTGC ATCCTCTCAC AACACATATC TCATGGGAGA TCAGTTAATG
    TCTCAGTCTC GAGTAGACAT GTACGCGTGG GTATTGCAAG CCGGTTGTCG TTGTGTTGAG
    GTGGACTGTT GGGACGGACC TGATGGAGAA CCAATAGTTC ATCATGGCTA TACTTTAACC
    TCTAAAATTC TTTTCAAAGA TGTGATAGAA ACAATTAACA AATATGCCTT CATCAGAAAT
    GACTACCCTG TCATCTTGTC TATTGAAAAT CACTGCAGTG TGGTACAGCA GAAGAAGATG
    GCTCAGTACC TGATTGAAAT CTTGGGCGAG AAATTAAATC TCTCTGATGT AGTCAATGAA
    GATTCAACAA AACTGCCGTC ACCGGAAAGC TTAAGGGGAA AAATATTAGT GAAGGGAAAG
    AAGCTTCCTG CCAGTATCAG CGATGACGCA GAGGAAGGAG ATGTTTCAGA TGAAGACAGT
    GCAGATGAGA TTGATGATGA CTGCAAGTTA ATAAATGGCG ATTGGAAGGC ATCTGCTAAC
    AGAAAGCAAG TGGAAAACAT TGCAAAGAAG AAACTTGACT CGCTGTTGAA AGAATCAAAA
    ATCCGGGACC GCGAAGATCC TAACAATTTC ACAGTCACCA CACTGCCACC ATCTGGGAAA
    CTCAGCCAGA AACCAGAATT AAAAAAGAAA TGCAGCTCTG GATCTAAAGG GGAAAGGGCT
    TTGAGTAAAA CAGAGGAAGA AGCTGAGTCT GGAGAAGAGC CGAGCCCCAA CAAAAGACAC
    AGTCGTTCTT TGATAGGAAG TTTTTCCAAG CACAAGAAAA AAGGTGGAAA AATAAAAAAA
    TGCTCAAGCT TAGAAGAAGG GGACGAAAGC CTGGACCGCC AGGGAAGTTT AGCAAGAGCT
    TCAGTACATT ATAGCAGAAT AAATAGACAA AAGAAAACCA TGAAGCTGTC AAGAGCCCTG
    TCTGACCTGG TAAAATACAC CAAATCAGTT GGTATCCATG ATGTGGAAAC TGAAATCTCT
    TGCAGCTGGC AAGTTTCATC ATTTAGTGAA ACAAAAGCCC ACCAGATCCT TCAGCAAAAA
    CCAGCTCAGT TTGTCCGGTT TAACCAACAC CAGCTCTCTC GCATTTATCC ATCGTCTTAC
    AGAGTCGACT CCAGCAACTA CAACCCACAA CCATTTTGGA ATGCTGGTTG TCAATTAGTT
    GCTTTAAACT ACCAGTCTGA AGGAAGAATG TTGCAGTTAA ACAGAGCCAA GTTCAACAGC
    AATGGAAACT GCGGTTATGT GCTGAAACCG AAATGCATGT GTCAAGGTGC CTTTAATCCT
    AATTCTGAAG ATCCTTTGCC AGGACAATTG AAGAAACAGC TGGTTTTGCG AATAATCAGT
    GGCCAACAGC TTCCAAAGCC AAAAGACTCC ATGCTGGGAG ATCGTGGAGA GATCATTGAT
    CCTTTTGTAG AAGTTGAGCT TATTGGTTTG CCAGTGGATT GCTTTAAAGA ACAAACACGA
    GTTGTTGATG ATAATGGATT CAATCCTATG TGGGAGGAAA CCCTTGTATT TACAGTGCAC
    ATGCCAGAAA TATCATTAAT TCGCTTCCTG GTGTGGGATC ATGATCCGAT TGGCCGGGAT
    TTCATTGGAC AACGGACTCT TTCATTTAGC AGCATGATGC CAGGCTATCG ACATGTGTAC
    CTGGAAGGTA TGGAGGAGGC ATCCATCTTT GTTCATGTAG CAATCAATGA CATCAGTGGT
    AAGGTCAAAC AGGCCGCGGG CTTAAAAGGA CTCTTTCTGA GAAATCCAAA GCAAGCTTCT
    CTGGACAGTC ATGCTGCTGG ACAGCTCCAT CGGAAACATT CCATTAGTAG TCACATTTTA
    AGGAGAACTG CAAGTGCTCC TACAAAGGGC CAACAAAAAA ACAAAAAGGG CTTCCCAGAA
    ATTGCCATTG ACTCCAAAGA AAACAGCTCG GATGGACCTA GTGAAGACAG GGAGACTGAT
    GATTCGTCTC TGCCCCGCTT TGAAAAAGAT CCTGAAAGTG CTTCTCCAGC TCTTCCTTCT
    AGGGATGGAA CTAATGTGGA ATTAAACAGC AAGGGTTTAA AAGACCCTCG ACAATTATCA
    ATTTATAGAT CGGCCTCCTC TTTATGCAGT CTTGAAACCA TTAAAGAAGA GCCTCTCTTT
    GCCAAGGAAA AACCTTCCAC TAGTCCCCAG CCTGTTGCCT GTTTTACAAG TTGTGAAAGC
    ACAGCATGTG ATCAGTACAA GGAATCTCAG TCTGAATGCA GTAGCCCAGC TATTACCTCT
    GAGAATGTAC AACGACCAAG TAAACTGAAT GTTGAGCCAG CCATAATCAA GGTGCTTGCT
    ATAAGTAATG GACTACATCC ACATAATGGA AAACTTAATC CAGGAAAAGC ATACAATGAC
    AGGAATGGAA AAGTACTCAG TTCTGTCAGT GGGAAAAGTG ATTGCACAAA TCTAATTAAT
    TCATCCCTGA TTAAGCCTGG GTTGTCTTTA CTTGCTGATG TTTCAAGAAA TGTCAATGAA
    CAATATCAAG TTAAAGCTAA CCAAGCTGCA CAATCTTCCA TTCATGCTAG TAATACTTCA
    GTTGCAAACA GAATAATGTG CACTAATCTA CCTGATCATC AAACTAGTTC AAAACCAAAC
    ATGAAACATC TGATTGTTCC TCCTGTTCAA AGATCACAGT ATGACAGGCC TGACTCAAAA
    CCTTCTGTGG TTCATGCTGG CAAACACACA CCTGTAAGGA GAGCAAAGAG TGAGGGCTAT
    GTTGTTTCAG ACTGCTCAGC CTTAAGCTTA AATTCTACAT GTCAGTCCCC TGTCGCTGAA
    GTATATTCTG ATGCTACAGT TAATGATAGA ATTTGGAGCA AACTGGAGTT AAACTCTCAT
    AGGGACAGTA TGTCTTCGTC TTCCAGCAAG TCATCCAATG ACACAGTCAT TGATCTGTCT
    TTGCCTAATT TAGCCCGGAA AAGCTTATTG GATCTTGACA TCTCTGATGA TAACTTTGAA
    AATATACATT TAAACAATAC TATTCGGCCA AGATCCGCTA CATCTTCAAC AAAGGAAATG
    TCGCTAGGAG TTTCAAAAAG TAAGTCTAAC CCTAATTTAG TATCCAATAA CCAAGCAATA
    GATGAACTCT CTTCTAGACC ACTGGCTAGA AGTCCTACAG AAGCATTGCC ACACTTGCCA
    CGAAGGAACA CCTGGAATAG ATTATACGTA GATAGTTTAA AACAGGCAAA TAAAAAGCCC
    AGTTTTGAGA ATAAACCACC AACTAACCAG TCAATGTCGA AAAGTCTTGG AGATCTGACT
    TCAGAAGATA TTTCATGTAA TTTTGAAAGT AAGTACAGAA GTATAAGCAG AAGTTTTATT
    ACTAGATCAA TGAGGGCACA AAGAAGACCG GCCAGTATGA AGGGTTCAGA GAAGTCTTCC
    GATTCACTGA CCGAACAGCT GAAAAAGCTA ACAAGCTTTC AGAAAGAAAA TGATATTACA
    TCCCCAACTG ATTTTGAACC TGTTCATTGT AACAATGATG ACACAGCCTT CCTTCGAAGG
    ACGTCCTCCA GAAGTCAGAG TAGAGTAAGA TATATTGCAA ACCGAGCCAA GCAGGCTCAA
    GAAAGGCAAA GGCTCCAAAA CCTGTGCCGG ATAAATGGAA ATTCCATAGA AGAAAGAGGG
    AATCCAGAAG GGGCATGTGC AATAACTAAA GGAATCTGTA TGGATTCTCT TTCTCCCACA
    CAGTTTACAT CTGAAACATT GGACAAATTT GGACCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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