Plch2 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006538936.2	XP_006538999.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
XM_011250268.1	XP_011248570.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
NM_175556.4	NP_780765.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform a
XM_011250271.2	XP_011248573.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
XM_011250276.1	XP_011248578.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X11
XM_011250269.2	XP_011248571.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
XM_011250271.3	XP_011248573.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
XM_011250272.1	XP_011248574.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
XM_011250274.2	XP_011248576.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X8
XM_017320247.1	XP_017175736.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X9
XM_011250275.1	XP_011248577.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X10
NM_001356470.1	NP_001343399.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform c
XM_011250270.1	XP_011248572.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
XM_011250273.2	XP_011248575.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
XM_006538940.2	XP_006539003.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X7
XM_011250276.2	XP_011248578.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X11
NM_001113360.3	NP_001106831.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform b
NM_001113360.2	NP_001106831.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform b
XM_006538944.3	XP_006539007.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X12
NM_175556.5	NP_780765.2	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform a
XM_011250275.2	XP_011248577.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu74429	XM_011250275.2 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu74430	XM_011250276.2 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu17405	NM_175556.5 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu34265	NM_001356470.1 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu34265	XM_006538944.3 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu74425	XM_011250271.3 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu16963	NM_001113360.3 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OMu74424	XM_011250269.2 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu74425	XM_011250270.1 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu74427	XM_011250273.2 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu34258	XM_006538936.2 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu34261	XM_006538940.2 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu74423	XM_011250268.1 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OMu74426	XM_011250272.1 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu74428	XM_011250274.2 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu81007	XM_017320247.1 Latest version!	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OMu74425	XM_011250271.2	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu74430	XM_011250276.1	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OMu16963	NM_001113360.2	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OMu17405	NM_175556.4	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu74429	XM_011250275.1	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

Plch2 ( XM_011250275.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248577.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu74429
Clone ID Related Accession (Same CDS sequence)	XM_011250275.1 , XM_011250275.2
Accession Version	XM_011250275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGTGGGCT 
TCTCTATGTC CCAAGTCTAC TTTTAGAAGC TCAGCCTTGT TAGGAGTCTC AAAGGTACCC 
GGCGCCGCGG GACGGTGTAG CCCGCCGCCC CACCTGCATG CCAGGCCGCG CCCATTAGCA 
TCCGTGATAC TTGGTAGCAT TTTGGAGACT GTCAGCTCAT GTGACATGGA TCATGACACG 
TTTCTGGGGT CCCCTGGGCC ATCCTCAGCC AGAGAGTGGC ATTTACGAGG CAGATCCAGG 
ATTCAGCCAA AGCCAAGGAC CTATCCTCCT ATCCTGTCCT CACCCTGTGC ACCTTCTGCC 
CTTCCCCATG ACTGTAGGTC TCTCTGCACA GCCTTGCAGC TCCTCTGCCG TCCTGTCCTC 
TCTACCTGCC TACACTGTCA GTTACTGGCC CCTCTAGCTC TCTCCTCTGT GTATGCTAGG 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248577.1
CDS	16..3621
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKWASLCPKSTFRSSALLGVSKVPGAAGRCSPPPHLHARPRPLASVILGSILETVSSCDMDHDTFLGSPGPSSAREWHLRGRSRIQPKPRTYPPILSSPCAPSALPHDCRSLCTALQLLCRPVLSTCLHCQLLAPLALSSVYARI

Target ORF information:

RefSeq Version	XM_011250275.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250275.2

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGTGGGCT 
TCTCTATGTC CCAAGTCTAC TTTTAGAAGC TCAGCCTTGT TAGGAGTCTC AAAGGTACCC 
GGCGCCGCGG GACGGTGTAG CCCGCCGCCC CACCTGCATG CCAGGCCGCG CCCATTAGCA 
TCCGTGATAC TTGGTAGCAT TTTGGAGACT GTCAGCTCAT GTGACATGGA TCATGACACG 
TTTCTGGGGT CCCCTGGGCC ATCCTCAGCC AGAGAGTGGC ATTTACGAGG CAGATCCAGG 
ATTCAGCCAA AGCCAAGGAC CTATCCTCCT ATCCTGTCCT CACCCTGTGC ACCTTCTGCC 
CTTCCCCATG ACTGTAGGTC TCTCTGCACA GCCTTGCAGC TCCTCTGCCG TCCTGTCCTC 
TCTACCTGCC TACACTGTCA GTTACTGGCC CCTCTAGCTC TCTCCTCTGT GTATGCTAGG 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250276.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248578.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu74430
Clone ID Related Accession (Same CDS sequence)	XM_011250276.1 , XM_011250276.2
Accession Version	XM_011250276.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGTTAGGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248578.1
CDS	16..3498
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRVRD

Target ORF information:

RefSeq Version	XM_011250276.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250276.2

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGTTAGGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( NM_175556.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780765.2 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 1, mRNA.

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CloneID	OMu17405
Clone ID Related Accession (Same CDS sequence)	NM_175556.4 , NM_175556.5
Accession Version	NM_175556.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DQ176851.1, AK044619.1, BX004788.7, BC052329.1, BC060083.1 and BG261709.1. On Apr 20, 2010 this sequence version replaced NM_175556.3. Transcript Variant: This variant (1) uses an alternate splice site and contains an alternate exon in the 3' coding region, resulting in an early stop codon, compared to variant 1. It encodes isoform a, which is shorter and has a distinct C-terminus, compared to isoform b. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC TGGCCTGGGG ACCATCCAGG GCCGCAGGCA GCAGTTGGGT CAATGCCAGT 
GGGACCTGGG AACAGCCCTT GCGGGGCTTC AGTGGGCTCC AAGGAGGAAG ACGAAGAGGT 
AGAGGAGAGA AGGGGATCCC TGAGGAGCCC CTGTGCCAGC TGACACCTCA GCTTGGCCTG 
TCGCTGAGGG TCCCTTTTGG CTTGGGGGAC TACGGCTTGG ATATGCCTGG TCCCCAGCCG 
TCTGCTGCCA GCCAGACCAC AGGAGCCGTG GCTTGCCTGG CAGAGGTACT CCTCTGGGTT 
GGAGGGAGCG TGGTAGTGTC ACCAAGATGG CAGCTCAGCC TTGTAGTGGA GCGATGCATG 
AGTGCCATGC AAGAGGGGAC CCAGATGGTG AAGCTCCGTG GCAGCTCCAA GGGATTGGTC 
CGCTTCTACT ACCTGGATGA GCACCGCTCC TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC 
GAGAAAGCCA AGATTTCCAT CGACTCCATC CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA 
ATCTTCCAGC GCTACCCGGA CAGCAGCTTT GACCCCAACT GCTGCTTCAG CATCTACCAT 
GGCAGCCACA GAGAGTCGCT GGACTTGGTC TCACCCAGCA GTGAAGAGGC ACGTACCTGG 
GTCACCGGCC TTCGCTACCT CATGGCTGGC ATCAGCGATG AAGACAGCCT AGCCCGTCGC 
CAGCGTACCA GGGACCAGTG GCTGAAGCAG ACGTTTGATG AGGCTGACAA GAACGGGGAC 
GGCAGCCTGA GCATCAGTGA GGTTCTGCAG CTGCTACATA AACTCAACGT GAACCTGCCC 
CGGCAGAGGG TGAAACAGAT GTTCAGGGAG GCGGACACAG ATGACCATCA GGGGACATTA 
GGCTTTGAGG AATTCTGCGC CTTCTACAAG ATGATGTCTA CCCGCCGAGA CCTCTACCTG 
CTCATGCTGA CCTACAGCAA CCATAAGGAC CACTTGGATG CCTCCGACCT GCAGCGCTTT 
CTGGAGGTGG AGCAGAAGAT GAACGGTGTG ACCCTGGAGA GCTGTCAGAA CATCATTGAG 
CAGTTTGAGC CTTGCCTGGA AAATAAGAGC AAGGGGATGC TGGGGATTGA TGGCTTTACC 
AACTACACCC GGAGCCCCGC CGGTGACATC TTCAACCCTG AGCACAACAG AGTGCACCAA 
GACATGACGC AGCCGCTGAG TCACTACTTC ATCACCTCGT CCCACAACAC CTACCTCGTG 
GGTGACCAGC TCATGTCCCA GTCTCGGGTG GACATGTATG CCTGGGTCCT GCAGGCCGGC 
TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC GGGCCCGATG GAGAACCCAT TGTCCATCAT 
GGCTACACTC TGACCTCCAA GATCCTTTTC AAAGATGTCA TCGAAACCAT CAACAAATAC 
GCCTTCATCA AGAATGAGTA CCCAGTGATC CTGTCCATTG AGAACCACTG TAGTGTTGTT 
CAGCAGAAGA AGATGGCCCA GTATCTGACT GACATCCTCG GGGACAAGCT GGACCTCTCC 
TCAGTGAGCA GCGAAGATGC CACCATGCTT CCATCTCCAC AGATGCTCAA GGGCAAGATC 
CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA 
TCTGATGAGG ACAGTGCTGA TGAGATGGAG GATGACTGCA AGCTCCTCAA TGGGGATGCC 
TCCACCAATC GGAAGCGTGT GGAAAACATT GCAAAGAAGA AGCTGGATTC TCTGATCAAG 
GAGTCAAAGA TCCGTGACTG TGAAGACCCC AATGACTTCT CTGTGTCCAC ACTATCCCCC 
TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC 
GTGGAGGCCG GGGAAGACAG CGGGGTGAGC AGGCAGAATA GCCGCCTCTT CATGAGCAGC 
TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG 
GACGAGACCC TGGACTCCCC AGGAAGCCAG AGCCGAGGGA CTGCCCGGCA AAAGAAGACC 
ATGAAGCTGT CACGAGCCCT CTCGGACCTA GTGAAATATA CCAAGTCTGT GGGGACCCAT 
GACGTGGAGA TAGAGGTGGT ATCTAGCTGG CAGGTGTCGT CCTTCAGTGA GACCAAGGCC 
CATCAGATCC TGCAGCAGAA GCCCACCCAG TACCTGCGCT TCAACCAGCA CCAGCTCTCG 
CGCATATACC CCTCCTCCTA CCGTGTGGAC TCCAGCAACT ACAATCCACA ACCCTTCTGG 
AACGCTGGTT GCCAGATGGT TGCCCTGAAC TACCAGTCAG AGGGGCGGAT GCTACAGCTG 
AACAGGGCCA AGTTCAGCGC CAACGGTGAC TGTGGCTACG TGCTCAAACC CCAGTGCATG 
TGCCAGGGTG TCTTCAACCC CAACTCGGAG GATCCCCTGC CGGGGCAGCT CAAGAAGCAG 
CTGGCCCTGA GGATCATCAG TGGCCAGCAG CTGCCCAAAC CACGGGACTC GGTGCTGGGC 
GACCGTGGGG AGATCATCGA CCCCTTCGTG GAGGTGGAGG TCATTGGGCT CCCCGTGGAC 
TGCAGCAAGG AGCAGACCCG AGTGGTGGAC GACAACGGAT TCAACCCCAT GTGGGAGGAG 
ACACTGGTGT TCACCGTGCA CATGCCAGAG ATTGCGCTTG TACGCTTCCT GGTCTGGGAC 
CATGACCCCA TTGGACGTGA CTTCATCGGC CAGAGGACAC TAGCCTTCAG CAGCATAATG 
CCAGGCTACC GGCATGTGTA CCTAGAAGGG ATGGAAGAGG CCTCTATCTT TGTTCATGTG 
GCTGTCAGTG ACATTAGTGG TAAGGTCAAG CAGACTCTGG GTCTAAAAGG TCTCTTCCTC 
CGAGGCACAA AGCCAGGCTC GCTGGACAGT CATGCTGCTG GACAGCCCCT CCCCCGGCCC 
TCCGTTAGCC AGAGGCTCCT GCGGCGCACG GCCAGTGCCC CGACCAAAAG CCAGAAGCCA 
AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG GGCACACAGG ACGCAGGCTC CGAGGGGGCA 
GCTGATGACG TGGCACCCTC TAGCCCCAAC CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG 
TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA CCAGGAGGAG AAGCAACAGA AGAGAGGACA 
CTGGCACAGG TGCGGTCCCC AAATGCCCCC GAAGGCCCTG GACCTGCAGG GATGGCCGCC 
ACATGCATGA AGTGTGTGGT GGGCTCCTGC GCCGGTATGG ACGTTGAGGG CCTTCAAAGG 
GAGCAACAAC CCAGCCCTGG GCCTGCAGGC AGCCACATGG CCATCAGCCA TCAGCCCAGG 
GCCCGGGTAG ATTCACTGGG GGGCCCTTGC TGCAGCCCAA GTCCTCGAGC CACCCCAGGG 
AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT 
GTGTCCTCGG ACTCCAGCAG CCCAGACAGC CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG 
CCGGAGGGTG CTCACAGGCA GCAGGGGGCG CTGCAGGGAG AGATGAACGC CTTGTTCGTT 
CAAAAGCTGG AGGAGATCAG GAGTCATTCC CCCATGTTCT CCACCGTTAG GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780765.2
CDS	1..3717
Translation	MGGLAWGPSRAAGSSWVNASGTWEQPLRGFSGLQGGRRRGRGEKGIPEEPLCQLTPQLGLSLRVPFGLGDYGLDMPGPQPSAASQTTGAVACLAEVLLWVGGSVVVSPRWQLSLVVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTVRD

Target ORF information:

RefSeq Version	NM_175556.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175556.4

ORF Insert Sequence:

ATGGGCGGAC TGGCCTGGGG ACCATCCAGG GCCGCAGGCA GCAGTTGGGT CAATGCCAGT 
GGGACCTGGG AACAGCCCTT GCGGGGCTTC AGTGGGCTCC AAGGAGGAAG ACGAAGAGGT 
AGAGGAGAGA AGGGGATCCC TGAGGAGCCC CTGTGCCAGC TGACACCTCA GCTTGGCCTG 
TCGCTGAGGG TCCCTTTTGG CTTGGGGGAC TACGGCTTGG ATATGCCTGG TCCCCAGCCG 
TCTGCTGCCA GCCAGACCAC AGGAGCCGTG GCTTGCCTGG CAGAGGTACT CCTCTGGGTT 
GGAGGGAGCG TGGTAGTGTC ACCAAGATGG CAGCTCAGCC TTGTAGTGGA GCGATGCATG 
AGTGCCATGC AAGAGGGGAC CCAGATGGTG AAGCTCCGTG GCAGCTCCAA GGGATTGGTC 
CGCTTCTACT ACCTGGATGA GCACCGCTCC TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC 
GAGAAAGCCA AGATTTCCAT CGACTCCATC CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA 
ATCTTCCAGC GCTACCCGGA CAGCAGCTTT GACCCCAACT GCTGCTTCAG CATCTACCAT 
GGCAGCCACA GAGAGTCGCT GGACTTGGTC TCACCCAGCA GTGAAGAGGC ACGTACCTGG 
GTCACCGGCC TTCGCTACCT CATGGCTGGC ATCAGCGATG AAGACAGCCT AGCCCGTCGC 
CAGCGTACCA GGGACCAGTG GCTGAAGCAG ACGTTTGATG AGGCTGACAA GAACGGGGAC 
GGCAGCCTGA GCATCAGTGA GGTTCTGCAG CTGCTACATA AACTCAACGT GAACCTGCCC 
CGGCAGAGGG TGAAACAGAT GTTCAGGGAG GCGGACACAG ATGACCATCA GGGGACATTA 
GGCTTTGAGG AATTCTGCGC CTTCTACAAG ATGATGTCTA CCCGCCGAGA CCTCTACCTG 
CTCATGCTGA CCTACAGCAA CCATAAGGAC CACTTGGATG CCTCCGACCT GCAGCGCTTT 
CTGGAGGTGG AGCAGAAGAT GAACGGTGTG ACCCTGGAGA GCTGTCAGAA CATCATTGAG 
CAGTTTGAGC CTTGCCTGGA AAATAAGAGC AAGGGGATGC TGGGGATTGA TGGCTTTACC 
AACTACACCC GGAGCCCCGC CGGTGACATC TTCAACCCTG AGCACAACAG AGTGCACCAA 
GACATGACGC AGCCGCTGAG TCACTACTTC ATCACCTCGT CCCACAACAC CTACCTCGTG 
GGTGACCAGC TCATGTCCCA GTCTCGGGTG GACATGTATG CCTGGGTCCT GCAGGCCGGC 
TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC GGGCCCGATG GAGAACCCAT TGTCCATCAT 
GGCTACACTC TGACCTCCAA GATCCTTTTC AAAGATGTCA TCGAAACCAT CAACAAATAC 
GCCTTCATCA AGAATGAGTA CCCAGTGATC CTGTCCATTG AGAACCACTG TAGTGTTGTT 
CAGCAGAAGA AGATGGCCCA GTATCTGACT GACATCCTCG GGGACAAGCT GGACCTCTCC 
TCAGTGAGCA GCGAAGATGC CACCATGCTT CCATCTCCAC AGATGCTCAA GGGCAAGATC 
CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA 
TCTGATGAGG ACAGTGCTGA TGAGATGGAG GATGACTGCA AGCTCCTCAA TGGGGATGCC 
TCCACCAATC GGAAGCGTGT GGAAAACATT GCAAAGAAGA AGCTGGATTC TCTGATCAAG 
GAGTCAAAGA TCCGTGACTG TGAAGACCCC AATGACTTCT CTGTGTCCAC ACTATCCCCC 
TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC 
GTGGAGGCCG GGGAAGACAG CGGGGTGAGC AGGCAGAATA GCCGCCTCTT CATGAGCAGC 
TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG 
GACGAGACCC TGGACTCCCC AGGAAGCCAG AGCCGAGGGA CTGCCCGGCA AAAGAAGACC 
ATGAAGCTGT CACGAGCCCT CTCGGACCTA GTGAAATATA CCAAGTCTGT GGGGACCCAT 
GACGTGGAGA TAGAGGTGGT ATCTAGCTGG CAGGTGTCGT CCTTCAGTGA GACCAAGGCC 
CATCAGATCC TGCAGCAGAA GCCCACCCAG TACCTGCGCT TCAACCAGCA CCAGCTCTCG 
CGCATATACC CCTCCTCCTA CCGTGTGGAC TCCAGCAACT ACAATCCACA ACCCTTCTGG 
AACGCTGGTT GCCAGATGGT TGCCCTGAAC TACCAGTCAG AGGGGCGGAT GCTACAGCTG 
AACAGGGCCA AGTTCAGCGC CAACGGTGAC TGTGGCTACG TGCTCAAACC CCAGTGCATG 
TGCCAGGGTG TCTTCAACCC CAACTCGGAG GATCCCCTGC CGGGGCAGCT CAAGAAGCAG 
CTGGCCCTGA GGATCATCAG TGGCCAGCAG CTGCCCAAAC CACGGGACTC GGTGCTGGGC 
GACCGTGGGG AGATCATCGA CCCCTTCGTG GAGGTGGAGG TCATTGGGCT CCCCGTGGAC 
TGCAGCAAGG AGCAGACCCG AGTGGTGGAC GACAACGGAT TCAACCCCAT GTGGGAGGAG 
ACACTGGTGT TCACCGTGCA CATGCCAGAG ATTGCGCTTG TACGCTTCCT GGTCTGGGAC 
CATGACCCCA TTGGACGTGA CTTCATCGGC CAGAGGACAC TAGCCTTCAG CAGCATAATG 
CCAGGCTACC GGCATGTGTA CCTAGAAGGG ATGGAAGAGG CCTCTATCTT TGTTCATGTG 
GCTGTCAGTG ACATTAGTGG TAAGGTCAAG CAGACTCTGG GTCTAAAAGG TCTCTTCCTC 
CGAGGCACAA AGCCAGGCTC GCTGGACAGT CATGCTGCTG GACAGCCCCT CCCCCGGCCC 
TCCGTTAGCC AGAGGCTCCT GCGGCGCACG GCCAGTGCCC CGACCAAAAG CCAGAAGCCA 
AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG GGCACACAGG ACGCAGGCTC CGAGGGGGCA 
GCTGATGACG TGGCACCCTC TAGCCCCAAC CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG 
TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA CCAGGAGGAG AAGCAACAGA AGAGAGGACA 
CTGGCACAGG TGCGGTCCCC AAATGCCCCC GAAGGCCCTG GACCTGCAGG GATGGCCGCC 
ACATGCATGA AGTGTGTGGT GGGCTCCTGC GCCGGTATGG ACGTTGAGGG CCTTCAAAGG 
GAGCAACAAC CCAGCCCTGG GCCTGCAGGC AGCCACATGG CCATCAGCCA TCAGCCCAGG 
GCCCGGGTAG ATTCACTGGG GGGCCCTTGC TGCAGCCCAA GTCCTCGAGC CACCCCAGGG 
AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT 
GTGTCCTCGG ACTCCAGCAG CCCAGACAGC CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG 
CCGGAGGGTG CTCACAGGCA GCAGGGGGCG CTGCAGGGAG AGATGAACGC CTTGTTCGTT 
CAAAAGCTGG AGGAGATCAG GAGTCATTCC CCCATGTTCT CCACCGTTAG GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( NM_175556.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780765.2 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 1, mRNA.

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CloneID	OMu17405
Clone ID Related Accession (Same CDS sequence)	NM_175556.4 , NM_175556.5
Accession Version	NM_175556.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-18
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL831788.14 and BX004788.7. On Jun 19, 2019 this sequence version replaced NM_175556.4. Transcript Variant: This variant (1) uses an alternate splice site and contains an alternate exon in the 3' coding region, resulting in an early stop codon, compared to variant 1. It encodes isoform a, which is shorter and has a distinct C-terminus, compared to isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK044619.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC TGGCCTGGGG ACCATCCAGG GCCGCAGGCA GCAGTTGGGT CAATGCCAGT 
GGGACCTGGG AACAGCCCTT GCGGGGCTTC AGTGGGCTCC AAGGAGGAAG ACGAAGAGGT 
AGAGGAGAGA AGGGGATCCC TGAGGAGCCC CTGTGCCAGC TGACACCTCA GCTTGGCCTG 
TCGCTGAGGG TCCCTTTTGG CTTGGGGGAC TACGGCTTGG ATATGCCTGG TCCCCAGCCG 
TCTGCTGCCA GCCAGACCAC AGGAGCCGTG GCTTGCCTGG CAGAGGTACT CCTCTGGGTT 
GGAGGGAGCG TGGTAGTGTC ACCAAGATGG CAGCTCAGCC TTGTAGTGGA GCGATGCATG 
AGTGCCATGC AAGAGGGGAC CCAGATGGTG AAGCTCCGTG GCAGCTCCAA GGGATTGGTC 
CGCTTCTACT ACCTGGATGA GCACCGCTCC TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC 
GAGAAAGCCA AGATTTCCAT CGACTCCATC CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA 
ATCTTCCAGC GCTACCCGGA CAGCAGCTTT GACCCCAACT GCTGCTTCAG CATCTACCAT 
GGCAGCCACA GAGAGTCGCT GGACTTGGTC TCACCCAGCA GTGAAGAGGC ACGTACCTGG 
GTCACCGGCC TTCGCTACCT CATGGCTGGC ATCAGCGATG AAGACAGCCT AGCCCGTCGC 
CAGCGTACCA GGGACCAGTG GCTGAAGCAG ACGTTTGATG AGGCTGACAA GAACGGGGAC 
GGCAGCCTGA GCATCAGTGA GGTTCTGCAG CTGCTACATA AACTCAACGT GAACCTGCCC 
CGGCAGAGGG TGAAACAGAT GTTCAGGGAG GCGGACACAG ATGACCATCA GGGGACATTA 
GGCTTTGAGG AATTCTGCGC CTTCTACAAG ATGATGTCTA CCCGCCGAGA CCTCTACCTG 
CTCATGCTGA CCTACAGCAA CCATAAGGAC CACTTGGATG CCTCCGACCT GCAGCGCTTT 
CTGGAGGTGG AGCAGAAGAT GAACGGTGTG ACCCTGGAGA GCTGTCAGAA CATCATTGAG 
CAGTTTGAGC CTTGCCTGGA AAATAAGAGC AAGGGGATGC TGGGGATTGA TGGCTTTACC 
AACTACACCC GGAGCCCCGC CGGTGACATC TTCAACCCTG AGCACAACAG AGTGCACCAA 
GACATGACGC AGCCGCTGAG TCACTACTTC ATCACCTCGT CCCACAACAC CTACCTCGTG 
GGTGACCAGC TCATGTCCCA GTCTCGGGTG GACATGTATG CCTGGGTCCT GCAGGCCGGC 
TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC GGGCCCGATG GAGAACCCAT TGTCCATCAT 
GGCTACACTC TGACCTCCAA GATCCTTTTC AAAGATGTCA TCGAAACCAT CAACAAATAC 
GCCTTCATCA AGAATGAGTA CCCAGTGATC CTGTCCATTG AGAACCACTG TAGTGTTGTT 
CAGCAGAAGA AGATGGCCCA GTATCTGACT GACATCCTCG GGGACAAGCT GGACCTCTCC 
TCAGTGAGCA GCGAAGATGC CACCATGCTT CCATCTCCAC AGATGCTCAA GGGCAAGATC 
CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA 
TCTGATGAGG ACAGTGCTGA TGAGATGGAG GATGACTGCA AGCTCCTCAA TGGGGATGCC 
TCCACCAATC GGAAGCGTGT GGAAAACATT GCAAAGAAGA AGCTGGATTC TCTGATCAAG 
GAGTCAAAGA TCCGTGACTG TGAAGACCCC AATGACTTCT CTGTGTCCAC ACTATCCCCC 
TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC 
GTGGAGGCCG GGGAAGACAG CGGGGTGAGC AGGCAGAATA GCCGCCTCTT CATGAGCAGC 
TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG 
GACGAGACCC TGGACTCCCC AGGAAGCCAG AGCCGAGGGA CTGCCCGGCA AAAGAAGACC 
ATGAAGCTGT CACGAGCCCT CTCGGACCTA GTGAAATATA CCAAGTCTGT GGGGACCCAT 
GACGTGGAGA TAGAGGTGGT ATCTAGCTGG CAGGTGTCGT CCTTCAGTGA GACCAAGGCC 
CATCAGATCC TGCAGCAGAA GCCCACCCAG TACCTGCGCT TCAACCAGCA CCAGCTCTCG 
CGCATATACC CCTCCTCCTA CCGTGTGGAC TCCAGCAACT ACAATCCACA ACCCTTCTGG 
AACGCTGGTT GCCAGATGGT TGCCCTGAAC TACCAGTCAG AGGGGCGGAT GCTACAGCTG 
AACAGGGCCA AGTTCAGCGC CAACGGTGAC TGTGGCTACG TGCTCAAACC CCAGTGCATG 
TGCCAGGGTG TCTTCAACCC CAACTCGGAG GATCCCCTGC CGGGGCAGCT CAAGAAGCAG 
CTGGCCCTGA GGATCATCAG TGGCCAGCAG CTGCCCAAAC CACGGGACTC GGTGCTGGGC 
GACCGTGGGG AGATCATCGA CCCCTTCGTG GAGGTGGAGG TCATTGGGCT CCCCGTGGAC 
TGCAGCAAGG AGCAGACCCG AGTGGTGGAC GACAACGGAT TCAACCCCAT GTGGGAGGAG 
ACACTGGTGT TCACCGTGCA CATGCCAGAG ATTGCGCTTG TACGCTTCCT GGTCTGGGAC 
CATGACCCCA TTGGACGTGA CTTCATCGGC CAGAGGACAC TAGCCTTCAG CAGCATAATG 
CCAGGCTACC GGCATGTGTA CCTAGAAGGG ATGGAAGAGG CCTCTATCTT TGTTCATGTG 
GCTGTCAGTG ACATTAGTGG TAAGGTCAAG CAGACTCTGG GTCTAAAAGG TCTCTTCCTC 
CGAGGCACAA AGCCAGGCTC GCTGGACAGT CATGCTGCTG GACAGCCCCT CCCCCGGCCC 
TCCGTTAGCC AGAGGCTCCT GCGGCGCACG GCCAGTGCCC CGACCAAAAG CCAGAAGCCA 
AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG GGCACACAGG ACGCAGGCTC CGAGGGGGCA 
GCTGATGACG TGGCACCCTC TAGCCCCAAC CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG 
TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA CCAGGAGGAG AAGCAACAGA AGAGAGGACA 
CTGGCACAGG TGCGGTCCCC AAATGCCCCC GAAGGCCCTG GACCTGCAGG GATGGCCGCC 
ACATGCATGA AGTGTGTGGT GGGCTCCTGC GCCGGTATGG ACGTTGAGGG CCTTCAAAGG 
GAGCAACAAC CCAGCCCTGG GCCTGCAGGC AGCCACATGG CCATCAGCCA TCAGCCCAGG 
GCCCGGGTAG ATTCACTGGG GGGCCCTTGC TGCAGCCCAA GTCCTCGAGC CACCCCAGGG 
AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT 
GTGTCCTCGG ACTCCAGCAG CCCAGACAGC CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG 
CCGGAGGGTG CTCACAGGCA GCAGGGGGCG CTGCAGGGAG AGATGAACGC CTTGTTCGTT 
CAAAAGCTGG AGGAGATCAG GAGTCATTCC CCCATGTTCT CCACCGTTAG GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780765.2
CDS	42..3758
Translation	MGGLAWGPSRAAGSSWVNASGTWEQPLRGFSGLQGGRRRGRGEKGIPEEPLCQLTPQLGLSLRVPFGLGDYGLDMPGPQPSAASQTTGAVACLAEVLLWVGGSVVVSPRWQLSLVVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTVRD

Target ORF information:

RefSeq Version	NM_175556.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175556.5

ORF Insert Sequence:

ATGGGCGGAC TGGCCTGGGG ACCATCCAGG GCCGCAGGCA GCAGTTGGGT CAATGCCAGT 
GGGACCTGGG AACAGCCCTT GCGGGGCTTC AGTGGGCTCC AAGGAGGAAG ACGAAGAGGT 
AGAGGAGAGA AGGGGATCCC TGAGGAGCCC CTGTGCCAGC TGACACCTCA GCTTGGCCTG 
TCGCTGAGGG TCCCTTTTGG CTTGGGGGAC TACGGCTTGG ATATGCCTGG TCCCCAGCCG 
TCTGCTGCCA GCCAGACCAC AGGAGCCGTG GCTTGCCTGG CAGAGGTACT CCTCTGGGTT 
GGAGGGAGCG TGGTAGTGTC ACCAAGATGG CAGCTCAGCC TTGTAGTGGA GCGATGCATG 
AGTGCCATGC AAGAGGGGAC CCAGATGGTG AAGCTCCGTG GCAGCTCCAA GGGATTGGTC 
CGCTTCTACT ACCTGGATGA GCACCGCTCC TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC 
GAGAAAGCCA AGATTTCCAT CGACTCCATC CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA 
ATCTTCCAGC GCTACCCGGA CAGCAGCTTT GACCCCAACT GCTGCTTCAG CATCTACCAT 
GGCAGCCACA GAGAGTCGCT GGACTTGGTC TCACCCAGCA GTGAAGAGGC ACGTACCTGG 
GTCACCGGCC TTCGCTACCT CATGGCTGGC ATCAGCGATG AAGACAGCCT AGCCCGTCGC 
CAGCGTACCA GGGACCAGTG GCTGAAGCAG ACGTTTGATG AGGCTGACAA GAACGGGGAC 
GGCAGCCTGA GCATCAGTGA GGTTCTGCAG CTGCTACATA AACTCAACGT GAACCTGCCC 
CGGCAGAGGG TGAAACAGAT GTTCAGGGAG GCGGACACAG ATGACCATCA GGGGACATTA 
GGCTTTGAGG AATTCTGCGC CTTCTACAAG ATGATGTCTA CCCGCCGAGA CCTCTACCTG 
CTCATGCTGA CCTACAGCAA CCATAAGGAC CACTTGGATG CCTCCGACCT GCAGCGCTTT 
CTGGAGGTGG AGCAGAAGAT GAACGGTGTG ACCCTGGAGA GCTGTCAGAA CATCATTGAG 
CAGTTTGAGC CTTGCCTGGA AAATAAGAGC AAGGGGATGC TGGGGATTGA TGGCTTTACC 
AACTACACCC GGAGCCCCGC CGGTGACATC TTCAACCCTG AGCACAACAG AGTGCACCAA 
GACATGACGC AGCCGCTGAG TCACTACTTC ATCACCTCGT CCCACAACAC CTACCTCGTG 
GGTGACCAGC TCATGTCCCA GTCTCGGGTG GACATGTATG CCTGGGTCCT GCAGGCCGGC 
TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC GGGCCCGATG GAGAACCCAT TGTCCATCAT 
GGCTACACTC TGACCTCCAA GATCCTTTTC AAAGATGTCA TCGAAACCAT CAACAAATAC 
GCCTTCATCA AGAATGAGTA CCCAGTGATC CTGTCCATTG AGAACCACTG TAGTGTTGTT 
CAGCAGAAGA AGATGGCCCA GTATCTGACT GACATCCTCG GGGACAAGCT GGACCTCTCC 
TCAGTGAGCA GCGAAGATGC CACCATGCTT CCATCTCCAC AGATGCTCAA GGGCAAGATC 
CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA 
TCTGATGAGG ACAGTGCTGA TGAGATGGAG GATGACTGCA AGCTCCTCAA TGGGGATGCC 
TCCACCAATC GGAAGCGTGT GGAAAACATT GCAAAGAAGA AGCTGGATTC TCTGATCAAG 
GAGTCAAAGA TCCGTGACTG TGAAGACCCC AATGACTTCT CTGTGTCCAC ACTATCCCCC 
TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC 
GTGGAGGCCG GGGAAGACAG CGGGGTGAGC AGGCAGAATA GCCGCCTCTT CATGAGCAGC 
TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG 
GACGAGACCC TGGACTCCCC AGGAAGCCAG AGCCGAGGGA CTGCCCGGCA AAAGAAGACC 
ATGAAGCTGT CACGAGCCCT CTCGGACCTA GTGAAATATA CCAAGTCTGT GGGGACCCAT 
GACGTGGAGA TAGAGGTGGT ATCTAGCTGG CAGGTGTCGT CCTTCAGTGA GACCAAGGCC 
CATCAGATCC TGCAGCAGAA GCCCACCCAG TACCTGCGCT TCAACCAGCA CCAGCTCTCG 
CGCATATACC CCTCCTCCTA CCGTGTGGAC TCCAGCAACT ACAATCCACA ACCCTTCTGG 
AACGCTGGTT GCCAGATGGT TGCCCTGAAC TACCAGTCAG AGGGGCGGAT GCTACAGCTG 
AACAGGGCCA AGTTCAGCGC CAACGGTGAC TGTGGCTACG TGCTCAAACC CCAGTGCATG 
TGCCAGGGTG TCTTCAACCC CAACTCGGAG GATCCCCTGC CGGGGCAGCT CAAGAAGCAG 
CTGGCCCTGA GGATCATCAG TGGCCAGCAG CTGCCCAAAC CACGGGACTC GGTGCTGGGC 
GACCGTGGGG AGATCATCGA CCCCTTCGTG GAGGTGGAGG TCATTGGGCT CCCCGTGGAC 
TGCAGCAAGG AGCAGACCCG AGTGGTGGAC GACAACGGAT TCAACCCCAT GTGGGAGGAG 
ACACTGGTGT TCACCGTGCA CATGCCAGAG ATTGCGCTTG TACGCTTCCT GGTCTGGGAC 
CATGACCCCA TTGGACGTGA CTTCATCGGC CAGAGGACAC TAGCCTTCAG CAGCATAATG 
CCAGGCTACC GGCATGTGTA CCTAGAAGGG ATGGAAGAGG CCTCTATCTT TGTTCATGTG 
GCTGTCAGTG ACATTAGTGG TAAGGTCAAG CAGACTCTGG GTCTAAAAGG TCTCTTCCTC 
CGAGGCACAA AGCCAGGCTC GCTGGACAGT CATGCTGCTG GACAGCCCCT CCCCCGGCCC 
TCCGTTAGCC AGAGGCTCCT GCGGCGCACG GCCAGTGCCC CGACCAAAAG CCAGAAGCCA 
AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG GGCACACAGG ACGCAGGCTC CGAGGGGGCA 
GCTGATGACG TGGCACCCTC TAGCCCCAAC CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG 
TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA CCAGGAGGAG AAGCAACAGA AGAGAGGACA 
CTGGCACAGG TGCGGTCCCC AAATGCCCCC GAAGGCCCTG GACCTGCAGG GATGGCCGCC 
ACATGCATGA AGTGTGTGGT GGGCTCCTGC GCCGGTATGG ACGTTGAGGG CCTTCAAAGG 
GAGCAACAAC CCAGCCCTGG GCCTGCAGGC AGCCACATGG CCATCAGCCA TCAGCCCAGG 
GCCCGGGTAG ATTCACTGGG GGGCCCTTGC TGCAGCCCAA GTCCTCGAGC CACCCCAGGG 
AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT 
GTGTCCTCGG ACTCCAGCAG CCCAGACAGC CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG 
CCGGAGGGTG CTCACAGGCA GCAGGGGGCG CTGCAGGGAG AGATGAACGC CTTGTTCGTT 
CAAAAGCTGG AGGAGATCAG GAGTCATTCC CCCATGTTCT CCACCGTTAG GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( NM_001356470.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343399.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 3, mRNA.

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CloneID	OMu34265
Clone ID Related Accession (Same CDS sequence)	NM_001356470.1 , XM_006538944.3
Accession Version	NM_001356470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-18
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ113070.1, AK044619.1, AL831788.14, DQ176851.1, BX004788.7, BC052329.1 and BC060083.1. On Oct 5, 2017 this sequence version replaced XM_006538944.3. ##Evidence-Data-START## Transcript exon combination :: AK129147.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGC CTGGGCCCCC TGGTGGGCTT AGCCAGGACC AAGTGGAGCG ATGCATGAGT 
GCCATGCAAG AGGGGACCCA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG ATTGGTCCGC 
TTCTACTACC TGGATGAGCA CCGCTCCTGC CTCCGATGGC GTCCCTCCCG CAAGAACGAG 
AAAGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGCCGGCA GTCTGAAATC 
TTCCAGCGCT ACCCGGACAG CAGCTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACAGAG AGTCGCTGGA CTTGGTCTCA CCCAGCAGTG AAGAGGCACG TACCTGGGTC 
ACCGGCCTTC GCTACCTCAT GGCTGGCATC AGCGATGAAG ACAGCCTAGC CCGTCGCCAG 
CGTACCAGGG ACCAGTGGCT GAAGCAGACG TTTGATGAGG CTGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCAGTGAGGT TCTGCAGCTG CTACATAAAC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AACAGATGTT CAGGGAGGCG GACACAGATG ACCATCAGGG GACATTAGGC 
TTTGAGGAAT TCTGCGCCTT CTACAAGATG ATGTCTACCC GCCGAGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA TAAGGACCAC TTGGATGCCT CCGACCTGCA GCGCTTTCTG 
GAGGTGGAGC AGAAGATGAA CGGTGTGACC CTGGAGAGCT GTCAGAACAT CATTGAGCAG 
TTTGAGCCTT GCCTGGAAAA TAAGAGCAAG GGGATGCTGG GGATTGATGG TAGTCGTGAG 
CCGAGCCCAG TGATGGAGAT ACACCACGGC GCTCCTGTGT ACACCGCAGG CTTTACCAAC 
TACACCCGGA GCCCCGCCGG TGACATCTTC AACCCTGAGC ACAACAGAGT GCACCAAGAC 
ATGACGCAGC CGCTGAGTCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTGGAC ATGTATGCCT GGGTCCTGCA GGCCGGCTGC 
CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCCGATGGAG AACCCATTGT CCATCATGGC 
TACACTCTGA CCTCCAAGAT CCTTTTCAAA GATGTCATCG AAACCATCAA CAAATACGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGTAG TGTTGTTCAG 
CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATCCTCGGGG ACAAGCTGGA CCTCTCCTCA 
GTGAGCAGCG AAGATGCCAC CATGCTTCCA TCTCCACAGA TGCTCAAGGG CAAGATCCTG 
GTGAAGGGGA AGAAGCTCCC TGCCAATATC AGTGAGGATG CAGAAGAAGG CGAAGTATCT 
GATGAGGACA GTGCTGATGA GATGGAGGAT GACTGCAAGC TCCTCAATGG GGATGCCTCC 
ACCAATCGGA AGCGTGTGGA AAACATTGCA AAGAAGAAGC TGGATTCTCT GATCAAGGAG 
TCAAAGATCC GTGACTGTGA AGACCCCAAT GACTTCTCTG TGTCCACACT ATCCCCCTCT 
GGAAAGCTTG GGCGCAAAGC AGAGGCCAAG AAGGGTCAGA GCAAGGTTGA GGAAGACGTG 
GAGGCCGGGG AAGACAGCGG GGTGAGCAGG CAGAATAGCC GCCTCTTCAT GAGCAGCTTC 
TCCAAGCGCA AGAAAAAAGG CAGCAAGATA AAGAAGGTGG CCAGCGTGGA AGAGGGGGAC 
GAGACCCTGG ACTCCCCAGG AAGCCAGAGC CGAGGGACTG CCCGGCAAAA GAAGACCATG 
AAGCTGTCAC GAGCCCTCTC GGACCTAGTG AAATATACCA AGTCTGTGGG GACCCATGAC 
GTGGAGATAG AGGTGGTATC TAGCTGGCAG GTGTCGTCCT TCAGTGAGAC CAAGGCCCAT 
CAGATCCTGC AGCAGAAGCC CACCCAGTAC CTGCGCTTCA ACCAGCACCA GCTCTCGCGC 
ATATACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ATCCACAACC CTTCTGGAAC 
GCTGGTTGCC AGATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT ACAGCTGAAC 
AGGGCCAAGT TCAGCGCCAA CGGTGACTGT GGCTACGTGC TCAAACCCCA GTGCATGTGC 
CAGGGTGTCT TCAACCCCAA CTCGGAGGAT CCCCTGCCGG GGCAGCTCAA GAAGCAGCTG 
GCCCTGAGGA TCATCAGTGG CCAGCAGCTG CCCAAACCAC GGGACTCGGT GCTGGGCGAC 
CGTGGGGAGA TCATCGACCC CTTCGTGGAG GTGGAGGTCA TTGGGCTCCC CGTGGACTGC 
AGCAAGGAGC AGACCCGAGT GGTGGACGAC AACGGATTCA ACCCCATGTG GGAGGAGACA 
CTGGTGTTCA CCGTGCACAT GCCAGAGATT GCGCTTGTAC GCTTCCTGGT CTGGGACCAT 
GACCCCATTG GACGTGACTT CATCGGCCAG AGGACACTAG CCTTCAGCAG CATAATGCCA 
GGCTACCGGC ATGTGTACCT AGAAGGGATG GAAGAGGCCT CTATCTTTGT TCATGTGGCT 
GTCAGTGACA TTAGTGGTAA GGTCAAGCAG ACTCTGGGTC TAAAAGGTCT CTTCCTCCGA 
GGCACAAAGC CAGGCTCGCT GGACAGTCAT GCTGCTGGAC AGCCCCTCCC CCGGCCCTCC 
GTTAGCCAGA GGCTCCTGCG GCGCACGGCC AGTGCCCCGA CCAAAAGCCA GAAGCCAAGT 
CGCAAGGGCT TCCCAGAGCT GGCCCTGGGC ACACAGGACG CAGGCTCCGA GGGGGCAGCT 
GATGACGTGG CACCCTCTAG CCCCAACCCA GCTCTGGAGG CCCCTACTCA AGAGAGGTCA 
GGCAGCAGCA GCCCCCGAGG TAAGGCACCA GGAGGAGAAG CAACAGAAGA GAGGACACTG 
GCACAGGTGC GGTCCCCAAA TGCCCCCGAA GGCCCTGGAC CTGCAGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG CTCCTGCGCC GGTATGGACG TTGAGGGCCT TCAAAGGGAG 
CAACAACCCA GCCCTGGGCC TGCAGGCAGC CACATGGCCA TCAGCCATCA GCCCAGGGCC 
CGGGTAGATT CACTGGGGGG CCCTTGCTGC AGCCCAAGTC CTCGAGCCAC CCCAGGGAGA 
AGCAAAGAGG CCCCCAAGGG TCCTAGGGCT CGGCGCCAGG GTCCAGGCGG CGGCTCTGTG 
TCCTCGGACT CCAGCAGCCC AGACAGCCCA GGCAGCCCCA AGGTGGCCCC CTGCCAGCCG 
GAGGGTGCTC ACAGGCAGCA GGGGGCGCTG CAGGGAGAGA TGAACGCCTT GTTCGTTCAA 
AAGCTGGAGG AGATCAGGAG TCATTCCCCC ATGTTCTCCA CCGTTAGGGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343399.1
CDS	187..3660
Translation	MEEPGPPGGLSQDQVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTVRD

Target ORF information:

RefSeq Version	NM_001356470.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356470.1

ORF Insert Sequence:

ATGGAAGAGC CTGGGCCCCC TGGTGGGCTT AGCCAGGACC AAGTGGAGCG ATGCATGAGT 
GCCATGCAAG AGGGGACCCA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG ATTGGTCCGC 
TTCTACTACC TGGATGAGCA CCGCTCCTGC CTCCGATGGC GTCCCTCCCG CAAGAACGAG 
AAAGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGCCGGCA GTCTGAAATC 
TTCCAGCGCT ACCCGGACAG CAGCTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACAGAG AGTCGCTGGA CTTGGTCTCA CCCAGCAGTG AAGAGGCACG TACCTGGGTC 
ACCGGCCTTC GCTACCTCAT GGCTGGCATC AGCGATGAAG ACAGCCTAGC CCGTCGCCAG 
CGTACCAGGG ACCAGTGGCT GAAGCAGACG TTTGATGAGG CTGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCAGTGAGGT TCTGCAGCTG CTACATAAAC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AACAGATGTT CAGGGAGGCG GACACAGATG ACCATCAGGG GACATTAGGC 
TTTGAGGAAT TCTGCGCCTT CTACAAGATG ATGTCTACCC GCCGAGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA TAAGGACCAC TTGGATGCCT CCGACCTGCA GCGCTTTCTG 
GAGGTGGAGC AGAAGATGAA CGGTGTGACC CTGGAGAGCT GTCAGAACAT CATTGAGCAG 
TTTGAGCCTT GCCTGGAAAA TAAGAGCAAG GGGATGCTGG GGATTGATGG TAGTCGTGAG 
CCGAGCCCAG TGATGGAGAT ACACCACGGC GCTCCTGTGT ACACCGCAGG CTTTACCAAC 
TACACCCGGA GCCCCGCCGG TGACATCTTC AACCCTGAGC ACAACAGAGT GCACCAAGAC 
ATGACGCAGC CGCTGAGTCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTGGAC ATGTATGCCT GGGTCCTGCA GGCCGGCTGC 
CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCCGATGGAG AACCCATTGT CCATCATGGC 
TACACTCTGA CCTCCAAGAT CCTTTTCAAA GATGTCATCG AAACCATCAA CAAATACGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGTAG TGTTGTTCAG 
CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATCCTCGGGG ACAAGCTGGA CCTCTCCTCA 
GTGAGCAGCG AAGATGCCAC CATGCTTCCA TCTCCACAGA TGCTCAAGGG CAAGATCCTG 
GTGAAGGGGA AGAAGCTCCC TGCCAATATC AGTGAGGATG CAGAAGAAGG CGAAGTATCT 
GATGAGGACA GTGCTGATGA GATGGAGGAT GACTGCAAGC TCCTCAATGG GGATGCCTCC 
ACCAATCGGA AGCGTGTGGA AAACATTGCA AAGAAGAAGC TGGATTCTCT GATCAAGGAG 
TCAAAGATCC GTGACTGTGA AGACCCCAAT GACTTCTCTG TGTCCACACT ATCCCCCTCT 
GGAAAGCTTG GGCGCAAAGC AGAGGCCAAG AAGGGTCAGA GCAAGGTTGA GGAAGACGTG 
GAGGCCGGGG AAGACAGCGG GGTGAGCAGG CAGAATAGCC GCCTCTTCAT GAGCAGCTTC 
TCCAAGCGCA AGAAAAAAGG CAGCAAGATA AAGAAGGTGG CCAGCGTGGA AGAGGGGGAC 
GAGACCCTGG ACTCCCCAGG AAGCCAGAGC CGAGGGACTG CCCGGCAAAA GAAGACCATG 
AAGCTGTCAC GAGCCCTCTC GGACCTAGTG AAATATACCA AGTCTGTGGG GACCCATGAC 
GTGGAGATAG AGGTGGTATC TAGCTGGCAG GTGTCGTCCT TCAGTGAGAC CAAGGCCCAT 
CAGATCCTGC AGCAGAAGCC CACCCAGTAC CTGCGCTTCA ACCAGCACCA GCTCTCGCGC 
ATATACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ATCCACAACC CTTCTGGAAC 
GCTGGTTGCC AGATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT ACAGCTGAAC 
AGGGCCAAGT TCAGCGCCAA CGGTGACTGT GGCTACGTGC TCAAACCCCA GTGCATGTGC 
CAGGGTGTCT TCAACCCCAA CTCGGAGGAT CCCCTGCCGG GGCAGCTCAA GAAGCAGCTG 
GCCCTGAGGA TCATCAGTGG CCAGCAGCTG CCCAAACCAC GGGACTCGGT GCTGGGCGAC 
CGTGGGGAGA TCATCGACCC CTTCGTGGAG GTGGAGGTCA TTGGGCTCCC CGTGGACTGC 
AGCAAGGAGC AGACCCGAGT GGTGGACGAC AACGGATTCA ACCCCATGTG GGAGGAGACA 
CTGGTGTTCA CCGTGCACAT GCCAGAGATT GCGCTTGTAC GCTTCCTGGT CTGGGACCAT 
GACCCCATTG GACGTGACTT CATCGGCCAG AGGACACTAG CCTTCAGCAG CATAATGCCA 
GGCTACCGGC ATGTGTACCT AGAAGGGATG GAAGAGGCCT CTATCTTTGT TCATGTGGCT 
GTCAGTGACA TTAGTGGTAA GGTCAAGCAG ACTCTGGGTC TAAAAGGTCT CTTCCTCCGA 
GGCACAAAGC CAGGCTCGCT GGACAGTCAT GCTGCTGGAC AGCCCCTCCC CCGGCCCTCC 
GTTAGCCAGA GGCTCCTGCG GCGCACGGCC AGTGCCCCGA CCAAAAGCCA GAAGCCAAGT 
CGCAAGGGCT TCCCAGAGCT GGCCCTGGGC ACACAGGACG CAGGCTCCGA GGGGGCAGCT 
GATGACGTGG CACCCTCTAG CCCCAACCCA GCTCTGGAGG CCCCTACTCA AGAGAGGTCA 
GGCAGCAGCA GCCCCCGAGG TAAGGCACCA GGAGGAGAAG CAACAGAAGA GAGGACACTG 
GCACAGGTGC GGTCCCCAAA TGCCCCCGAA GGCCCTGGAC CTGCAGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG CTCCTGCGCC GGTATGGACG TTGAGGGCCT TCAAAGGGAG 
CAACAACCCA GCCCTGGGCC TGCAGGCAGC CACATGGCCA TCAGCCATCA GCCCAGGGCC 
CGGGTAGATT CACTGGGGGG CCCTTGCTGC AGCCCAAGTC CTCGAGCCAC CCCAGGGAGA 
AGCAAAGAGG CCCCCAAGGG TCCTAGGGCT CGGCGCCAGG GTCCAGGCGG CGGCTCTGTG 
TCCTCGGACT CCAGCAGCCC AGACAGCCCA GGCAGCCCCA AGGTGGCCCC CTGCCAGCCG 
GAGGGTGCTC ACAGGCAGCA GGGGGCGCTG CAGGGAGAGA TGAACGCCTT GTTCGTTCAA 
AAGCTGGAGG AGATCAGGAG TCATTCCCCC ATGTTCTCCA CCGTTAGGGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_006538944.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539007.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X14, mRNA.

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CloneID	OMu34265
Clone ID Related Accession (Same CDS sequence)	NM_001356470.1 , XM_006538944.3
Accession Version	XM_006538944.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538944.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC CTGGGCCCCC TGGTGGGCTT AGCCAGGACC AAGTGGAGCG ATGCATGAGT 
GCCATGCAAG AGGGGACCCA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG ATTGGTCCGC 
TTCTACTACC TGGATGAGCA CCGCTCCTGC CTCCGATGGC GTCCCTCCCG CAAGAACGAG 
AAAGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGCCGGCA GTCTGAAATC 
TTCCAGCGCT ACCCGGACAG CAGCTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACAGAG AGTCGCTGGA CTTGGTCTCA CCCAGCAGTG AAGAGGCACG TACCTGGGTC 
ACCGGCCTTC GCTACCTCAT GGCTGGCATC AGCGATGAAG ACAGCCTAGC CCGTCGCCAG 
CGTACCAGGG ACCAGTGGCT GAAGCAGACG TTTGATGAGG CTGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCAGTGAGGT TCTGCAGCTG CTACATAAAC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AACAGATGTT CAGGGAGGCG GACACAGATG ACCATCAGGG GACATTAGGC 
TTTGAGGAAT TCTGCGCCTT CTACAAGATG ATGTCTACCC GCCGAGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA TAAGGACCAC TTGGATGCCT CCGACCTGCA GCGCTTTCTG 
GAGGTGGAGC AGAAGATGAA CGGTGTGACC CTGGAGAGCT GTCAGAACAT CATTGAGCAG 
TTTGAGCCTT GCCTGGAAAA TAAGAGCAAG GGGATGCTGG GGATTGATGG TAGTCGTGAG 
CCGAGCCCAG TGATGGAGAT ACACCACGGC GCTCCTGTGT ACACCGCAGG CTTTACCAAC 
TACACCCGGA GCCCCGCCGG TGACATCTTC AACCCTGAGC ACAACAGAGT GCACCAAGAC 
ATGACGCAGC CGCTGAGTCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTGGAC ATGTATGCCT GGGTCCTGCA GGCCGGCTGC 
CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCCGATGGAG AACCCATTGT CCATCATGGC 
TACACTCTGA CCTCCAAGAT CCTTTTCAAA GATGTCATCG AAACCATCAA CAAATACGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGTAG TGTTGTTCAG 
CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATCCTCGGGG ACAAGCTGGA CCTCTCCTCA 
GTGAGCAGCG AAGATGCCAC CATGCTTCCA TCTCCACAGA TGCTCAAGGG CAAGATCCTG 
GTGAAGGGGA AGAAGCTCCC TGCCAATATC AGTGAGGATG CAGAAGAAGG CGAAGTATCT 
GATGAGGACA GTGCTGATGA GATGGAGGAT GACTGCAAGC TCCTCAATGG GGATGCCTCC 
ACCAATCGGA AGCGTGTGGA AAACATTGCA AAGAAGAAGC TGGATTCTCT GATCAAGGAG 
TCAAAGATCC GTGACTGTGA AGACCCCAAT GACTTCTCTG TGTCCACACT ATCCCCCTCT 
GGAAAGCTTG GGCGCAAAGC AGAGGCCAAG AAGGGTCAGA GCAAGGTTGA GGAAGACGTG 
GAGGCCGGGG AAGACAGCGG GGTGAGCAGG CAGAATAGCC GCCTCTTCAT GAGCAGCTTC 
TCCAAGCGCA AGAAAAAAGG CAGCAAGATA AAGAAGGTGG CCAGCGTGGA AGAGGGGGAC 
GAGACCCTGG ACTCCCCAGG AAGCCAGAGC CGAGGGACTG CCCGGCAAAA GAAGACCATG 
AAGCTGTCAC GAGCCCTCTC GGACCTAGTG AAATATACCA AGTCTGTGGG GACCCATGAC 
GTGGAGATAG AGGTGGTATC TAGCTGGCAG GTGTCGTCCT TCAGTGAGAC CAAGGCCCAT 
CAGATCCTGC AGCAGAAGCC CACCCAGTAC CTGCGCTTCA ACCAGCACCA GCTCTCGCGC 
ATATACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ATCCACAACC CTTCTGGAAC 
GCTGGTTGCC AGATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT ACAGCTGAAC 
AGGGCCAAGT TCAGCGCCAA CGGTGACTGT GGCTACGTGC TCAAACCCCA GTGCATGTGC 
CAGGGTGTCT TCAACCCCAA CTCGGAGGAT CCCCTGCCGG GGCAGCTCAA GAAGCAGCTG 
GCCCTGAGGA TCATCAGTGG CCAGCAGCTG CCCAAACCAC GGGACTCGGT GCTGGGCGAC 
CGTGGGGAGA TCATCGACCC CTTCGTGGAG GTGGAGGTCA TTGGGCTCCC CGTGGACTGC 
AGCAAGGAGC AGACCCGAGT GGTGGACGAC AACGGATTCA ACCCCATGTG GGAGGAGACA 
CTGGTGTTCA CCGTGCACAT GCCAGAGATT GCGCTTGTAC GCTTCCTGGT CTGGGACCAT 
GACCCCATTG GACGTGACTT CATCGGCCAG AGGACACTAG CCTTCAGCAG CATAATGCCA 
GGCTACCGGC ATGTGTACCT AGAAGGGATG GAAGAGGCCT CTATCTTTGT TCATGTGGCT 
GTCAGTGACA TTAGTGGTAA GGTCAAGCAG ACTCTGGGTC TAAAAGGTCT CTTCCTCCGA 
GGCACAAAGC CAGGCTCGCT GGACAGTCAT GCTGCTGGAC AGCCCCTCCC CCGGCCCTCC 
GTTAGCCAGA GGCTCCTGCG GCGCACGGCC AGTGCCCCGA CCAAAAGCCA GAAGCCAAGT 
CGCAAGGGCT TCCCAGAGCT GGCCCTGGGC ACACAGGACG CAGGCTCCGA GGGGGCAGCT 
GATGACGTGG CACCCTCTAG CCCCAACCCA GCTCTGGAGG CCCCTACTCA AGAGAGGTCA 
GGCAGCAGCA GCCCCCGAGG TAAGGCACCA GGAGGAGAAG CAACAGAAGA GAGGACACTG 
GCACAGGTGC GGTCCCCAAA TGCCCCCGAA GGCCCTGGAC CTGCAGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG CTCCTGCGCC GGTATGGACG TTGAGGGCCT TCAAAGGGAG 
CAACAACCCA GCCCTGGGCC TGCAGGCAGC CACATGGCCA TCAGCCATCA GCCCAGGGCC 
CGGGTAGATT CACTGGGGGG CCCTTGCTGC AGCCCAAGTC CTCGAGCCAC CCCAGGGAGA 
AGCAAAGAGG CCCCCAAGGG TCCTAGGGCT CGGCGCCAGG GTCCAGGCGG CGGCTCTGTG 
TCCTCGGACT CCAGCAGCCC AGACAGCCCA GGCAGCCCCA AGGTGGCCCC CTGCCAGCCG 
GAGGGTGCTC ACAGGCAGCA GGGGGCGCTG CAGGGAGAGA TGAACGCCTT GTTCGTTCAA 
AAGCTGGAGG AGATCAGGAG TCATTCCCCC ATGTTCTCCA CCGTTAGGGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539007.1
CDS	195..3668
Translation	MEEPGPPGGLSQDQVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTVRD

Target ORF information:

RefSeq Version	XM_006538944.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538944.3

ORF Insert Sequence:

ATGGAAGAGC CTGGGCCCCC TGGTGGGCTT AGCCAGGACC AAGTGGAGCG ATGCATGAGT 
GCCATGCAAG AGGGGACCCA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG ATTGGTCCGC 
TTCTACTACC TGGATGAGCA CCGCTCCTGC CTCCGATGGC GTCCCTCCCG CAAGAACGAG 
AAAGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGCCGGCA GTCTGAAATC 
TTCCAGCGCT ACCCGGACAG CAGCTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACAGAG AGTCGCTGGA CTTGGTCTCA CCCAGCAGTG AAGAGGCACG TACCTGGGTC 
ACCGGCCTTC GCTACCTCAT GGCTGGCATC AGCGATGAAG ACAGCCTAGC CCGTCGCCAG 
CGTACCAGGG ACCAGTGGCT GAAGCAGACG TTTGATGAGG CTGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCAGTGAGGT TCTGCAGCTG CTACATAAAC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AACAGATGTT CAGGGAGGCG GACACAGATG ACCATCAGGG GACATTAGGC 
TTTGAGGAAT TCTGCGCCTT CTACAAGATG ATGTCTACCC GCCGAGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA TAAGGACCAC TTGGATGCCT CCGACCTGCA GCGCTTTCTG 
GAGGTGGAGC AGAAGATGAA CGGTGTGACC CTGGAGAGCT GTCAGAACAT CATTGAGCAG 
TTTGAGCCTT GCCTGGAAAA TAAGAGCAAG GGGATGCTGG GGATTGATGG TAGTCGTGAG 
CCGAGCCCAG TGATGGAGAT ACACCACGGC GCTCCTGTGT ACACCGCAGG CTTTACCAAC 
TACACCCGGA GCCCCGCCGG TGACATCTTC AACCCTGAGC ACAACAGAGT GCACCAAGAC 
ATGACGCAGC CGCTGAGTCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTGGAC ATGTATGCCT GGGTCCTGCA GGCCGGCTGC 
CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCCGATGGAG AACCCATTGT CCATCATGGC 
TACACTCTGA CCTCCAAGAT CCTTTTCAAA GATGTCATCG AAACCATCAA CAAATACGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGTAG TGTTGTTCAG 
CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATCCTCGGGG ACAAGCTGGA CCTCTCCTCA 
GTGAGCAGCG AAGATGCCAC CATGCTTCCA TCTCCACAGA TGCTCAAGGG CAAGATCCTG 
GTGAAGGGGA AGAAGCTCCC TGCCAATATC AGTGAGGATG CAGAAGAAGG CGAAGTATCT 
GATGAGGACA GTGCTGATGA GATGGAGGAT GACTGCAAGC TCCTCAATGG GGATGCCTCC 
ACCAATCGGA AGCGTGTGGA AAACATTGCA AAGAAGAAGC TGGATTCTCT GATCAAGGAG 
TCAAAGATCC GTGACTGTGA AGACCCCAAT GACTTCTCTG TGTCCACACT ATCCCCCTCT 
GGAAAGCTTG GGCGCAAAGC AGAGGCCAAG AAGGGTCAGA GCAAGGTTGA GGAAGACGTG 
GAGGCCGGGG AAGACAGCGG GGTGAGCAGG CAGAATAGCC GCCTCTTCAT GAGCAGCTTC 
TCCAAGCGCA AGAAAAAAGG CAGCAAGATA AAGAAGGTGG CCAGCGTGGA AGAGGGGGAC 
GAGACCCTGG ACTCCCCAGG AAGCCAGAGC CGAGGGACTG CCCGGCAAAA GAAGACCATG 
AAGCTGTCAC GAGCCCTCTC GGACCTAGTG AAATATACCA AGTCTGTGGG GACCCATGAC 
GTGGAGATAG AGGTGGTATC TAGCTGGCAG GTGTCGTCCT TCAGTGAGAC CAAGGCCCAT 
CAGATCCTGC AGCAGAAGCC CACCCAGTAC CTGCGCTTCA ACCAGCACCA GCTCTCGCGC 
ATATACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ATCCACAACC CTTCTGGAAC 
GCTGGTTGCC AGATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT ACAGCTGAAC 
AGGGCCAAGT TCAGCGCCAA CGGTGACTGT GGCTACGTGC TCAAACCCCA GTGCATGTGC 
CAGGGTGTCT TCAACCCCAA CTCGGAGGAT CCCCTGCCGG GGCAGCTCAA GAAGCAGCTG 
GCCCTGAGGA TCATCAGTGG CCAGCAGCTG CCCAAACCAC GGGACTCGGT GCTGGGCGAC 
CGTGGGGAGA TCATCGACCC CTTCGTGGAG GTGGAGGTCA TTGGGCTCCC CGTGGACTGC 
AGCAAGGAGC AGACCCGAGT GGTGGACGAC AACGGATTCA ACCCCATGTG GGAGGAGACA 
CTGGTGTTCA CCGTGCACAT GCCAGAGATT GCGCTTGTAC GCTTCCTGGT CTGGGACCAT 
GACCCCATTG GACGTGACTT CATCGGCCAG AGGACACTAG CCTTCAGCAG CATAATGCCA 
GGCTACCGGC ATGTGTACCT AGAAGGGATG GAAGAGGCCT CTATCTTTGT TCATGTGGCT 
GTCAGTGACA TTAGTGGTAA GGTCAAGCAG ACTCTGGGTC TAAAAGGTCT CTTCCTCCGA 
GGCACAAAGC CAGGCTCGCT GGACAGTCAT GCTGCTGGAC AGCCCCTCCC CCGGCCCTCC 
GTTAGCCAGA GGCTCCTGCG GCGCACGGCC AGTGCCCCGA CCAAAAGCCA GAAGCCAAGT 
CGCAAGGGCT TCCCAGAGCT GGCCCTGGGC ACACAGGACG CAGGCTCCGA GGGGGCAGCT 
GATGACGTGG CACCCTCTAG CCCCAACCCA GCTCTGGAGG CCCCTACTCA AGAGAGGTCA 
GGCAGCAGCA GCCCCCGAGG TAAGGCACCA GGAGGAGAAG CAACAGAAGA GAGGACACTG 
GCACAGGTGC GGTCCCCAAA TGCCCCCGAA GGCCCTGGAC CTGCAGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG CTCCTGCGCC GGTATGGACG TTGAGGGCCT TCAAAGGGAG 
CAACAACCCA GCCCTGGGCC TGCAGGCAGC CACATGGCCA TCAGCCATCA GCCCAGGGCC 
CGGGTAGATT CACTGGGGGG CCCTTGCTGC AGCCCAAGTC CTCGAGCCAC CCCAGGGAGA 
AGCAAAGAGG CCCCCAAGGG TCCTAGGGCT CGGCGCCAGG GTCCAGGCGG CGGCTCTGTG 
TCCTCGGACT CCAGCAGCCC AGACAGCCCA GGCAGCCCCA AGGTGGCCCC CTGCCAGCCG 
GAGGGTGCTC ACAGGCAGCA GGGGGCGCTG CAGGGAGAGA TGAACGCCTT GTTCGTTCAA 
AAGCTGGAGG AGATCAGGAG TCATTCCCCC ATGTTCTCCA CCGTTAGGGA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250271.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248573.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X5, mRNA.

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CloneID	OMu74425
Clone ID Related Accession (Same CDS sequence)	XM_011250271.2 , XM_011250271.3 , XM_011250270.1
Accession Version	XM_011250271.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250271.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGCT CCATTGGTCC CGCTTCATTG CCTGAGGACC CAGGGCCGGA TCTAGGCAGC 
AGATGGCTTT TCCTGAGTGC CAACGTTTTG CCTGTGGTGG AGCGATGCAT GAGTGCCATG 
CAAGAGGGGA CCCAGATGGT GAAGCTCCGT GGCAGCTCCA AGGGATTGGT CCGCTTCTAC 
TACCTGGATG AGCACCGCTC CTGCCTCCGA TGGCGTCCCT CCCGCAAGAA CGAGAAAGCC 
AAGATTTCCA TCGACTCCAT CCAGGAGGTG AGTGAGGGCC GGCAGTCTGA AATCTTCCAG 
CGCTACCCGG ACAGCAGCTT TGACCCCAAC TGCTGCTTCA GCATCTACCA TGGCAGCCAC 
AGAGAGTCGC TGGACTTGGT CTCACCCAGC AGTGAAGAGG CACGTACCTG GGTCACCGGC 
CTTCGCTACC TCATGGCTGG CATCAGCGAT GAAGACAGCC TAGCCCGTCG CCAGCGTACC 
AGGGACCAGT GGCTGAAGCA GACGTTTGAT GAGGCTGACA AGAACGGGGA CGGCAGCCTG 
AGCATCAGTG AGGTTCTGCA GCTGCTACAT AAACTCAACG TGAACCTGCC CCGGCAGAGG 
GTGAAACAGA TGTTCAGGGA GGCGGACACA GATGACCATC AGGGGACATT AGGCTTTGAG 
GAATTCTGCG CCTTCTACAA GATGATGTCT ACCCGCCGAG ACCTCTACCT GCTCATGCTG 
ACCTACAGCA ACCATAAGGA CCACTTGGAT GCCTCCGACC TGCAGCGCTT TCTGGAGGTG 
GAGCAGAAGA TGAACGGTGT GACCCTGGAG AGCTGTCAGA ACATCATTGA GCAGTTTGAG 
CCTTGCCTGG AAAATAAGAG CAAGGGGATG CTGGGGATTG ATGGTAGTCG TGAGCCGAGC 
CCAGTGATGG AGATACACCA CGGCGCTCCT GTGTACACCG CAGGCTTTAC CAACTACACC 
CGGAGCCCCG CCGGTGACAT CTTCAACCCT GAGCACAACA GAGTGCACCA AGACATGACG 
CAGCCGCTGA GTCACTACTT CATCACCTCG TCCCACAACA CCTACCTCGT GGGTGACCAG 
CTCATGTCCC AGTCTCGGGT GGACATGTAT GCCTGGGTCC TGCAGGCCGG CTGCCGCTGT 
GTGGAGGTGG ACTGCTGGGA CGGGCCCGAT GGAGAACCCA TTGTCCATCA TGGCTACACT 
CTGACCTCCA AGATCCTTTT CAAAGATGTC ATCGAAACCA TCAACAAATA CGCCTTCATC 
AAGAATGAGT ACCCAGTGAT CCTGTCCATT GAGAACCACT GTAGTGTTGT TCAGCAGAAG 
AAGATGGCCC AGTATCTGAC TGACATCCTC GGGGACAAGC TGGACCTCTC CTCAGTGAGC 
AGCGAAGATG CCACCATGCT TCCATCTCCA CAGATGCTCA AGGGCAAGAT CCTGGTGAAG 
GGGAAGAAGC TCCCTGCCAA TATCAGTGAG GATGCAGAAG AAGGCGAAGT ATCTGATGAG 
GACAGTGCTG ATGAGATGGA GGATGACTGC AAGCTCCTCA ATGGGGATGC CTCCACCAAT 
CGGAAGCGTG TGGAAAACAT TGCAAAGAAG AAGCTGGATT CTCTGATCAA GGAGTCAAAG 
ATCCGTGACT GTGAAGACCC CAATGACTTC TCTGTGTCCA CACTATCCCC CTCTGGAAAG 
CTTGGGCGCA AAGCAGAGGC CAAGAAGGGT CAGAGCAAGG TTGAGGAAGA CGTGGAGGCC 
GGGGAAGACA GCGGGGTGAG CAGGCAGAAT AGCCGCCTCT TCATGAGCAG CTTCTCCAAG 
CGCAAGAAAA AAGGCAGCAA GATAAAGAAG GTGGCCAGCG TGGAAGAGGG GGACGAGACC 
CTGGACTCCC CAGGAAGCCA GAGCCGAGGG ACTGCCCGGC AAAAGAAGAC CATGAAGCTG 
TCACGAGCCC TCTCGGACCT AGTGAAATAT ACCAAGTCTG TGGGGACCCA TGACGTGGAG 
ATAGAGGTGG TATCTAGCTG GCAGGTGTCG TCCTTCAGTG AGACCAAGGC CCATCAGATC 
CTGCAGCAGA AGCCCACCCA GTACCTGCGC TTCAACCAGC ACCAGCTCTC GCGCATATAC 
CCCTCCTCCT ACCGTGTGGA CTCCAGCAAC TACAATCCAC AACCCTTCTG GAACGCTGGT 
TGCCAGATGG TTGCCCTGAA CTACCAGTCA GAGGGGCGGA TGCTACAGCT GAACAGGGCC 
AAGTTCAGCG CCAACGGTGA CTGTGGCTAC GTGCTCAAAC CCCAGTGCAT GTGCCAGGGT 
GTCTTCAACC CCAACTCGGA GGATCCCCTG CCGGGGCAGC TCAAGAAGCA GCTGGCCCTG 
AGGATCATCA GTGGCCAGCA GCTGCCCAAA CCACGGGACT CGGTGCTGGG CGACCGTGGG 
GAGATCATCG ACCCCTTCGT GGAGGTGGAG GTCATTGGGC TCCCCGTGGA CTGCAGCAAG 
GAGCAGACCC GAGTGGTGGA CGACAACGGA TTCAACCCCA TGTGGGAGGA GACACTGGTG 
TTCACCGTGC ACATGCCAGA GATTGCGCTT GTACGCTTCC TGGTCTGGGA CCATGACCCC 
ATTGGACGTG ACTTCATCGG CCAGAGGACA CTAGCCTTCA GCAGCATAAT GCCAGGCTAC 
CGGCATGTGT ACCTAGAAGG GATGGAAGAG GCCTCTATCT TTGTTCATGT GGCTGTCAGT 
GACATTAGTG GTAAGGTCAA GCAGACTCTG GGTCTAAAAG GTCTCTTCCT CCGAGGCACA 
AAGCCAGGCT CGCTGGACAG TCATGCTGCT GGACAGCCCC TCCCCCGGCC CTCCGTTAGC 
CAGAGGCTCC TGCGGCGCAC GGCCAGTGCC CCGACCAAAA GCCAGAAGCC AAGTCGCAAG 
GGCTTCCCAG AGCTGGCCCT GGGCACACAG GACGCAGGCT CCGAGGGGGC AGCTGATGAC 
GTGGCACCCT CTAGCCCCAA CCCAGCTCTG GAGGCCCCTA CTCAAGAGAG GTCAGGCAGC 
AGCAGCCCCC GAGGTAAGGC ACCAGGAGGA GAAGCAACAG AAGAGAGGAC ACTGGCACAG 
GTGCGGTCCC CAAATGCCCC CGAAGGCCCT GGACCTGCAG GGATGGCCGC CACATGCATG 
AAGTGTGTGG TGGGCTCCTG CGCCGGTATG GACGTTGAGG GCCTTCAAAG GGAGCAACAA 
CCCAGCCCTG GGCCTGCAGG CAGCCACATG GCCATCAGCC ATCAGCCCAG GGCCCGGGTA 
GATTCACTGG GGGGCCCTTG CTGCAGCCCA AGTCCTCGAG CCACCCCAGG GAGAAGCAAA 
GAGGCCCCCA AGGGTCCTAG GGCTCGGCGC CAGGGTCCAG GCGGCGGCTC TGTGTCCTCG 
GACTCCAGCA GCCCAGACAG CCCAGGCAGC CCCAAGGTGG CCCCCTGCCA GCCGGAGGGT 
GCTCACAGGC AGCAGGGGGC GCTGCAGGGA GAGATGAACG CCTTGTTCGT TCAAAAGCTG 
GAGGAGATCA GGAGTCATTC CCCCATGTTC TCCACCGATA CTCGCCTCTT CCCCCTGCAG 
CGGCCCATCT CCCCCCTGTG CAGCCTGGAA CCCATTGCCG AGGAACCAGC CCTGGGCCCT 
GGCCTCCCGT TACAGGCAGC TGCCCCCACA GGTCCATCTC AGGAAGGGTC CCAGTGCCCT 
GTAGGCCTGG GAGCCAAAGT GACCAGCTCC CAGCAGACAT CTCTGGGAGC TTTTGGAACC 
CTCCAGCTCA GGATTGGGGG CGGTAGGGAA AACGAAGAGC CACCCCTCAG GCCACACAAT 
GGTGGGATCT CCAGCGGGCC ACGTGAGGGG ACTTCTGGAA GACAGACAGA CAGCAAGAGC 
CGCTCCCGGG TCCCGGGCCA CTTGCCAGTG GTTAGGAGGG CCAAGAGTGA GGGCCAGGTG 
CTCTCGGAGC TCTCTCCCAC ACCTGCAGTG TACTCGGATG CCACGGGCAC TGACAGGTTA 
TGGCAGCGGC TGGAGCCTGG GAGCCACAGG GACAGTGTCT CCTCATCCTC CAGCATGTCC 
TCCAACGACA CCGTCATAGA CCTATCACTG CCCAGCCTCG GCCTCTGCCG CAGCCGAGAG 
AGCATTCCAG GGGTCTCTCT TGGACGCCTA ACCTCACGGC CCTGCTTAGC CTCAGCTGCC 
CGTCCTGACC TGCCTCCTGT GACCAAGAGC AAATCGAACC CCAACCTGCG GGTTGCGGGT 
GGGCTGCCTA CTGCACCCGA CGAGCTGCAG CCCCGGCCTC TTGCCCCGCG GCTGACCGGC 
CACCATCCAC GGCCACCGTG GCATCACCTC ACCCTGGTGG GCCTGCGGGA CTGCCCAGTG 
TCTGCTAAGT CCAAGAGTCT GGGAGACCTG ACAGCTGACG ACTTTGCTCC CAGCTTTCAG 
GGCAGCACCA GCAGCCTGAG CTGCGGCCTA GGCTCCCTGG GTGTGGCGCA TCAGGTGCTG 
GAGCCGGGAA TACGGCGAGA TGCTCTCACG GAGCAGCTAC GCTGGCTCAC CGGCTTCCAG 
CAGGCTGGGG ACATCACGTC GCCCACCAGC TTGGGCCCAG CTGGGGACGG GTCTGTAGGG 
GGCCCCAGCT TCCTGAGGCG TTCCTCCTCT CGGAGCCAGA GCCGCGTGCG CGCTATTGCC 
AGCCGAGCCC GCCAAGCGCA GGAGCGCCAG CAGAGGCTGA GAGGCCAGGA CTCACGGGGT 
CCCCCAGAGG AGGAGCGGGG CACCCCTGAG GGTGCCTGTT CTGTAGGCCA TGAGGGCTGT 
GTGGATGTGC CAATGCCTGC CAAGGGAGCC CCTGAGCAGG TGTGTGGTGC TGCTGATGGC 
CAGCTGCTGC TCAGGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248573.1
CDS	354..5174
Translation	MDSSIGPASLPEDPGPDLGSRWLFLSANVLPVVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	XM_011250271.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250271.3

ORF Insert Sequence:

ATGGATAGCT CCATTGGTCC CGCTTCATTG CCTGAGGACC CAGGGCCGGA TCTAGGCAGC 
AGATGGCTTT TCCTGAGTGC CAACGTTTTG CCTGTGGTGG AGCGATGCAT GAGTGCCATG 
CAAGAGGGGA CCCAGATGGT GAAGCTCCGT GGCAGCTCCA AGGGATTGGT CCGCTTCTAC 
TACCTGGATG AGCACCGCTC CTGCCTCCGA TGGCGTCCCT CCCGCAAGAA CGAGAAAGCC 
AAGATTTCCA TCGACTCCAT CCAGGAGGTG AGTGAGGGCC GGCAGTCTGA AATCTTCCAG 
CGCTACCCGG ACAGCAGCTT TGACCCCAAC TGCTGCTTCA GCATCTACCA TGGCAGCCAC 
AGAGAGTCGC TGGACTTGGT CTCACCCAGC AGTGAAGAGG CACGTACCTG GGTCACCGGC 
CTTCGCTACC TCATGGCTGG CATCAGCGAT GAAGACAGCC TAGCCCGTCG CCAGCGTACC 
AGGGACCAGT GGCTGAAGCA GACGTTTGAT GAGGCTGACA AGAACGGGGA CGGCAGCCTG 
AGCATCAGTG AGGTTCTGCA GCTGCTACAT AAACTCAACG TGAACCTGCC CCGGCAGAGG 
GTGAAACAGA TGTTCAGGGA GGCGGACACA GATGACCATC AGGGGACATT AGGCTTTGAG 
GAATTCTGCG CCTTCTACAA GATGATGTCT ACCCGCCGAG ACCTCTACCT GCTCATGCTG 
ACCTACAGCA ACCATAAGGA CCACTTGGAT GCCTCCGACC TGCAGCGCTT TCTGGAGGTG 
GAGCAGAAGA TGAACGGTGT GACCCTGGAG AGCTGTCAGA ACATCATTGA GCAGTTTGAG 
CCTTGCCTGG AAAATAAGAG CAAGGGGATG CTGGGGATTG ATGGTAGTCG TGAGCCGAGC 
CCAGTGATGG AGATACACCA CGGCGCTCCT GTGTACACCG CAGGCTTTAC CAACTACACC 
CGGAGCCCCG CCGGTGACAT CTTCAACCCT GAGCACAACA GAGTGCACCA AGACATGACG 
CAGCCGCTGA GTCACTACTT CATCACCTCG TCCCACAACA CCTACCTCGT GGGTGACCAG 
CTCATGTCCC AGTCTCGGGT GGACATGTAT GCCTGGGTCC TGCAGGCCGG CTGCCGCTGT 
GTGGAGGTGG ACTGCTGGGA CGGGCCCGAT GGAGAACCCA TTGTCCATCA TGGCTACACT 
CTGACCTCCA AGATCCTTTT CAAAGATGTC ATCGAAACCA TCAACAAATA CGCCTTCATC 
AAGAATGAGT ACCCAGTGAT CCTGTCCATT GAGAACCACT GTAGTGTTGT TCAGCAGAAG 
AAGATGGCCC AGTATCTGAC TGACATCCTC GGGGACAAGC TGGACCTCTC CTCAGTGAGC 
AGCGAAGATG CCACCATGCT TCCATCTCCA CAGATGCTCA AGGGCAAGAT CCTGGTGAAG 
GGGAAGAAGC TCCCTGCCAA TATCAGTGAG GATGCAGAAG AAGGCGAAGT ATCTGATGAG 
GACAGTGCTG ATGAGATGGA GGATGACTGC AAGCTCCTCA ATGGGGATGC CTCCACCAAT 
CGGAAGCGTG TGGAAAACAT TGCAAAGAAG AAGCTGGATT CTCTGATCAA GGAGTCAAAG 
ATCCGTGACT GTGAAGACCC CAATGACTTC TCTGTGTCCA CACTATCCCC CTCTGGAAAG 
CTTGGGCGCA AAGCAGAGGC CAAGAAGGGT CAGAGCAAGG TTGAGGAAGA CGTGGAGGCC 
GGGGAAGACA GCGGGGTGAG CAGGCAGAAT AGCCGCCTCT TCATGAGCAG CTTCTCCAAG 
CGCAAGAAAA AAGGCAGCAA GATAAAGAAG GTGGCCAGCG TGGAAGAGGG GGACGAGACC 
CTGGACTCCC CAGGAAGCCA GAGCCGAGGG ACTGCCCGGC AAAAGAAGAC CATGAAGCTG 
TCACGAGCCC TCTCGGACCT AGTGAAATAT ACCAAGTCTG TGGGGACCCA TGACGTGGAG 
ATAGAGGTGG TATCTAGCTG GCAGGTGTCG TCCTTCAGTG AGACCAAGGC CCATCAGATC 
CTGCAGCAGA AGCCCACCCA GTACCTGCGC TTCAACCAGC ACCAGCTCTC GCGCATATAC 
CCCTCCTCCT ACCGTGTGGA CTCCAGCAAC TACAATCCAC AACCCTTCTG GAACGCTGGT 
TGCCAGATGG TTGCCCTGAA CTACCAGTCA GAGGGGCGGA TGCTACAGCT GAACAGGGCC 
AAGTTCAGCG CCAACGGTGA CTGTGGCTAC GTGCTCAAAC CCCAGTGCAT GTGCCAGGGT 
GTCTTCAACC CCAACTCGGA GGATCCCCTG CCGGGGCAGC TCAAGAAGCA GCTGGCCCTG 
AGGATCATCA GTGGCCAGCA GCTGCCCAAA CCACGGGACT CGGTGCTGGG CGACCGTGGG 
GAGATCATCG ACCCCTTCGT GGAGGTGGAG GTCATTGGGC TCCCCGTGGA CTGCAGCAAG 
GAGCAGACCC GAGTGGTGGA CGACAACGGA TTCAACCCCA TGTGGGAGGA GACACTGGTG 
TTCACCGTGC ACATGCCAGA GATTGCGCTT GTACGCTTCC TGGTCTGGGA CCATGACCCC 
ATTGGACGTG ACTTCATCGG CCAGAGGACA CTAGCCTTCA GCAGCATAAT GCCAGGCTAC 
CGGCATGTGT ACCTAGAAGG GATGGAAGAG GCCTCTATCT TTGTTCATGT GGCTGTCAGT 
GACATTAGTG GTAAGGTCAA GCAGACTCTG GGTCTAAAAG GTCTCTTCCT CCGAGGCACA 
AAGCCAGGCT CGCTGGACAG TCATGCTGCT GGACAGCCCC TCCCCCGGCC CTCCGTTAGC 
CAGAGGCTCC TGCGGCGCAC GGCCAGTGCC CCGACCAAAA GCCAGAAGCC AAGTCGCAAG 
GGCTTCCCAG AGCTGGCCCT GGGCACACAG GACGCAGGCT CCGAGGGGGC AGCTGATGAC 
GTGGCACCCT CTAGCCCCAA CCCAGCTCTG GAGGCCCCTA CTCAAGAGAG GTCAGGCAGC 
AGCAGCCCCC GAGGTAAGGC ACCAGGAGGA GAAGCAACAG AAGAGAGGAC ACTGGCACAG 
GTGCGGTCCC CAAATGCCCC CGAAGGCCCT GGACCTGCAG GGATGGCCGC CACATGCATG 
AAGTGTGTGG TGGGCTCCTG CGCCGGTATG GACGTTGAGG GCCTTCAAAG GGAGCAACAA 
CCCAGCCCTG GGCCTGCAGG CAGCCACATG GCCATCAGCC ATCAGCCCAG GGCCCGGGTA 
GATTCACTGG GGGGCCCTTG CTGCAGCCCA AGTCCTCGAG CCACCCCAGG GAGAAGCAAA 
GAGGCCCCCA AGGGTCCTAG GGCTCGGCGC CAGGGTCCAG GCGGCGGCTC TGTGTCCTCG 
GACTCCAGCA GCCCAGACAG CCCAGGCAGC CCCAAGGTGG CCCCCTGCCA GCCGGAGGGT 
GCTCACAGGC AGCAGGGGGC GCTGCAGGGA GAGATGAACG CCTTGTTCGT TCAAAAGCTG 
GAGGAGATCA GGAGTCATTC CCCCATGTTC TCCACCGATA CTCGCCTCTT CCCCCTGCAG 
CGGCCCATCT CCCCCCTGTG CAGCCTGGAA CCCATTGCCG AGGAACCAGC CCTGGGCCCT 
GGCCTCCCGT TACAGGCAGC TGCCCCCACA GGTCCATCTC AGGAAGGGTC CCAGTGCCCT 
GTAGGCCTGG GAGCCAAAGT GACCAGCTCC CAGCAGACAT CTCTGGGAGC TTTTGGAACC 
CTCCAGCTCA GGATTGGGGG CGGTAGGGAA AACGAAGAGC CACCCCTCAG GCCACACAAT 
GGTGGGATCT CCAGCGGGCC ACGTGAGGGG ACTTCTGGAA GACAGACAGA CAGCAAGAGC 
CGCTCCCGGG TCCCGGGCCA CTTGCCAGTG GTTAGGAGGG CCAAGAGTGA GGGCCAGGTG 
CTCTCGGAGC TCTCTCCCAC ACCTGCAGTG TACTCGGATG CCACGGGCAC TGACAGGTTA 
TGGCAGCGGC TGGAGCCTGG GAGCCACAGG GACAGTGTCT CCTCATCCTC CAGCATGTCC 
TCCAACGACA CCGTCATAGA CCTATCACTG CCCAGCCTCG GCCTCTGCCG CAGCCGAGAG 
AGCATTCCAG GGGTCTCTCT TGGACGCCTA ACCTCACGGC CCTGCTTAGC CTCAGCTGCC 
CGTCCTGACC TGCCTCCTGT GACCAAGAGC AAATCGAACC CCAACCTGCG GGTTGCGGGT 
GGGCTGCCTA CTGCACCCGA CGAGCTGCAG CCCCGGCCTC TTGCCCCGCG GCTGACCGGC 
CACCATCCAC GGCCACCGTG GCATCACCTC ACCCTGGTGG GCCTGCGGGA CTGCCCAGTG 
TCTGCTAAGT CCAAGAGTCT GGGAGACCTG ACAGCTGACG ACTTTGCTCC CAGCTTTCAG 
GGCAGCACCA GCAGCCTGAG CTGCGGCCTA GGCTCCCTGG GTGTGGCGCA TCAGGTGCTG 
GAGCCGGGAA TACGGCGAGA TGCTCTCACG GAGCAGCTAC GCTGGCTCAC CGGCTTCCAG 
CAGGCTGGGG ACATCACGTC GCCCACCAGC TTGGGCCCAG CTGGGGACGG GTCTGTAGGG 
GGCCCCAGCT TCCTGAGGCG TTCCTCCTCT CGGAGCCAGA GCCGCGTGCG CGCTATTGCC 
AGCCGAGCCC GCCAAGCGCA GGAGCGCCAG CAGAGGCTGA GAGGCCAGGA CTCACGGGGT 
CCCCCAGAGG AGGAGCGGGG CACCCCTGAG GGTGCCTGTT CTGTAGGCCA TGAGGGCTGT 
GTGGATGTGC CAATGCCTGC CAAGGGAGCC CCTGAGCAGG TGTGTGGTGC TGCTGATGGC 
CAGCTGCTGC TCAGGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( NM_001113360.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001106831.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16963
Clone ID Related Accession (Same CDS sequence)	NM_001113360.3 , NM_001113360.2
Accession Version	NM_001113360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-18
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL831788.14 and BX004788.7. On Jun 19, 2019 this sequence version replaced NM_001113360.2. Transcript Variant: This variant (2) encodes the longest isoform (b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC TGGCCTGGGG ACCATCCAGG GCCGCAGGCA GCAGTTGGGT CAATGCCAGT 
GGGACCTGGG AACAGCCCTT GCGGGGCTTC AGTGGGCTCC AAGGAGGAAG ACGAAGAGGT 
AGAGGAGAGA AGGGGATCCC TGAGGAGCCC CTGTGCCAGC TGACACCTCA GCTTGGCCTG 
TCGCTGAGGG TCCCTTTTGG CTTGGGGGAC TACGGCTTGG ATATGCCTGG TCCCCAGCCG 
TCTGCTGCCA GCCAGACCAC AGGAGCCGTG GCTTGCCTGG CAGAGGTACT CCTCTGGGTT 
GGAGGGAGCG TGGTAGTGTC ACCAAGATGG CAGCTCAGCC TTGTAGTGGA GCGATGCATG 
AGTGCCATGC AAGAGGGGAC CCAGATGGTG AAGCTCCGTG GCAGCTCCAA GGGATTGGTC 
CGCTTCTACT ACCTGGATGA GCACCGCTCC TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC 
GAGAAAGCCA AGATTTCCAT CGACTCCATC CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA 
ATCTTCCAGC GCTACCCGGA CAGCAGCTTT GACCCCAACT GCTGCTTCAG CATCTACCAT 
GGCAGCCACA GAGAGTCGCT GGACTTGGTC TCACCCAGCA GTGAAGAGGC ACGTACCTGG 
GTCACCGGCC TTCGCTACCT CATGGCTGGC ATCAGCGATG AAGACAGCCT AGCCCGTCGC 
CAGCGTACCA GGGACCAGTG GCTGAAGCAG ACGTTTGATG AGGCTGACAA GAACGGGGAC 
GGCAGCCTGA GCATCAGTGA GGTTCTGCAG CTGCTACATA AACTCAACGT GAACCTGCCC 
CGGCAGAGGG TGAAACAGAT GTTCAGGGAG GCGGACACAG ATGACCATCA GGGGACATTA 
GGCTTTGAGG AATTCTGCGC CTTCTACAAG ATGATGTCTA CCCGCCGAGA CCTCTACCTG 
CTCATGCTGA CCTACAGCAA CCATAAGGAC CACTTGGATG CCTCCGACCT GCAGCGCTTT 
CTGGAGGTGG AGCAGAAGAT GAACGGTGTG ACCCTGGAGA GCTGTCAGAA CATCATTGAG 
CAGTTTGAGC CTTGCCTGGA AAATAAGAGC AAGGGGATGC TGGGGATTGA TGGCTTTACC 
AACTACACCC GGAGCCCCGC CGGTGACATC TTCAACCCTG AGCACAACAG AGTGCACCAA 
GACATGACGC AGCCGCTGAG TCACTACTTC ATCACCTCGT CCCACAACAC CTACCTCGTG 
GGTGACCAGC TCATGTCCCA GTCTCGGGTG GACATGTATG CCTGGGTCCT GCAGGCCGGC 
TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC GGGCCCGATG GAGAACCCAT TGTCCATCAT 
GGCTACACTC TGACCTCCAA GATCCTTTTC AAAGATGTCA TCGAAACCAT CAACAAATAC 
GCCTTCATCA AGAATGAGTA CCCAGTGATC CTGTCCATTG AGAACCACTG TAGTGTTGTT 
CAGCAGAAGA AGATGGCCCA GTATCTGACT GACATCCTCG GGGACAAGCT GGACCTCTCC 
TCAGTGAGCA GCGAAGATGC CACCATGCTT CCATCTCCAC AGATGCTCAA GGGCAAGATC 
CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA 
TCTGATGAGG ACAGTGCTGA TGAGATGGAG GATGACTGCA AGCTCCTCAA TGGGGATGCC 
TCCACCAATC GGAAGCGTGT GGAAAACATT GCAAAGAAGA AGCTGGATTC TCTGATCAAG 
GAGTCAAAGA TCCGTGACTG TGAAGACCCC AATGACTTCT CTGTGTCCAC ACTATCCCCC 
TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC 
GTGGAGGCCG GGGAAGACAG CGGGGTGAGC AGGCAGAATA GCCGCCTCTT CATGAGCAGC 
TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG 
GACGAGACCC TGGACTCCCC AGGAAGCCAG AGCCGAGGGA CTGCCCGGCA AAAGAAGACC 
ATGAAGCTGT CACGAGCCCT CTCGGACCTA GTGAAATATA CCAAGTCTGT GGGGACCCAT 
GACGTGGAGA TAGAGGTGGT ATCTAGCTGG CAGGTGTCGT CCTTCAGTGA GACCAAGGCC 
CATCAGATCC TGCAGCAGAA GCCCACCCAG TACCTGCGCT TCAACCAGCA CCAGCTCTCG 
CGCATATACC CCTCCTCCTA CCGTGTGGAC TCCAGCAACT ACAATCCACA ACCCTTCTGG 
AACGCTGGTT GCCAGATGGT TGCCCTGAAC TACCAGTCAG AGGGGCGGAT GCTACAGCTG 
AACAGGGCCA AGTTCAGCGC CAACGGTGAC TGTGGCTACG TGCTCAAACC CCAGTGCATG 
TGCCAGGGTG TCTTCAACCC CAACTCGGAG GATCCCCTGC CGGGGCAGCT CAAGAAGCAG 
CTGGCCCTGA GGATCATCAG TGGCCAGCAG CTGCCCAAAC CACGGGACTC GGTGCTGGGC 
GACCGTGGGG AGATCATCGA CCCCTTCGTG GAGGTGGAGG TCATTGGGCT CCCCGTGGAC 
TGCAGCAAGG AGCAGACCCG AGTGGTGGAC GACAACGGAT TCAACCCCAT GTGGGAGGAG 
ACACTGGTGT TCACCGTGCA CATGCCAGAG ATTGCGCTTG TACGCTTCCT GGTCTGGGAC 
CATGACCCCA TTGGACGTGA CTTCATCGGC CAGAGGACAC TAGCCTTCAG CAGCATAATG 
CCAGGCTACC GGCATGTGTA CCTAGAAGGG ATGGAAGAGG CCTCTATCTT TGTTCATGTG 
GCTGTCAGTG ACATTAGTGG TAAGGTCAAG CAGACTCTGG GTCTAAAAGG TCTCTTCCTC 
CGAGGCACAA AGCCAGGCTC GCTGGACAGT CATGCTGCTG GACAGCCCCT CCCCCGGCCC 
TCCGTTAGCC AGAGGCTCCT GCGGCGCACG GCCAGTGCCC CGACCAAAAG CCAGAAGCCA 
AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG GGCACACAGG ACGCAGGCTC CGAGGGGGCA 
GCTGATGACG TGGCACCCTC TAGCCCCAAC CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG 
TCAGGCAGCA GCAGCCCCCG AGATACTCGC CTCTTCCCCC TGCAGCGGCC CATCTCCCCC 
CTGTGCAGCC TGGAACCCAT TGCCGAGGAA CCAGCCCTGG GCCCTGGCCT CCCGTTACAG 
GCAGCTGCCC CCACAGGTCC ATCTCAGGAA GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC 
AAAGTGACCA GCTCCCAGCA GACATCTCTG GGAGCTTTTG GAACCCTCCA GCTCAGGATT 
GGGGGCGGTA GGGAAAACGA AGAGCCACCC CTCAGGCCAC ACAATGGTGG GATCTCCAGC 
GGGCCACGTG AGGGGACTTC TGGAAGACAG ACAGACAGCA AGAGCCGCTC CCGGGTCCCG 
GGCCACTTGC CAGTGGTTAG GAGGGCCAAG AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT 
CCCACACCTG CAGTGTACTC GGATGCCACG GGCACTGACA GGTTATGGCA GCGGCTGGAG 
CCTGGGAGCC ACAGGGACAG TGTCTCCTCA TCCTCCAGCA TGTCCTCCAA CGACACCGTC 
ATAGACCTAT CACTGCCCAG CCTCGGCCTC TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC 
TCTCTTGGAC GCCTAACCTC ACGGCCCTGC TTAGCCTCAG CTGCCCGTCC TGACCTGCCT 
CCTGTGACCA AGAGCAAATC GAACCCCAAC CTGCGGGTTG CGGGTGGGCT GCCTACTGCA 
CCCGACGAGC TGCAGCCCCG GCCTCTTGCC CCGCGGCTGA CCGGCCACCA TCCACGGCCA 
CCGTGGCATC ACCTCACCCT GGTGGGCCTG CGGGACTGCC CAGTGTCTGC TAAGTCCAAG 
AGTCTGGGAG ACCTGACAGC TGACGACTTT GCTCCCAGCT TTCAGGGCAG CACCAGCAGC 
CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG GCGCATCAGG TGCTGGAGCC GGGAATACGG 
CGAGATGCTC TCACGGAGCA GCTACGCTGG CTCACCGGCT TCCAGCAGGC TGGGGACATC 
ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG GACGGGTCTG TAGGGGGCCC CAGCTTCCTG 
AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA 
GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC CAGGACTCAC GGGGTCCCCC AGAGGAGGAG 
CGGGGCACCC CTGAGGGTGC CTGTTCTGTA GGCCATGAGG GCTGTGTGGA TGTGCCAATG 
CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106831.1
CDS	42..4547
Translation	MGGLAWGPSRAAGSSWVNASGTWEQPLRGFSGLQGGRRRGRGEKGIPEEPLCQLTPQLGLSLRVPFGLGDYGLDMPGPQPSAASQTTGAVACLAEVLLWVGGSVVVSPRWQLSLVVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	NM_001113360.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113360.3

ORF Insert Sequence:

ATGGGCGGAC TGGCCTGGGG ACCATCCAGG GCCGCAGGCA GCAGTTGGGT CAATGCCAGT 
GGGACCTGGG AACAGCCCTT GCGGGGCTTC AGTGGGCTCC AAGGAGGAAG ACGAAGAGGT 
AGAGGAGAGA AGGGGATCCC TGAGGAGCCC CTGTGCCAGC TGACACCTCA GCTTGGCCTG 
TCGCTGAGGG TCCCTTTTGG CTTGGGGGAC TACGGCTTGG ATATGCCTGG TCCCCAGCCG 
TCTGCTGCCA GCCAGACCAC AGGAGCCGTG GCTTGCCTGG CAGAGGTACT CCTCTGGGTT 
GGAGGGAGCG TGGTAGTGTC ACCAAGATGG CAGCTCAGCC TTGTAGTGGA GCGATGCATG 
AGTGCCATGC AAGAGGGGAC CCAGATGGTG AAGCTCCGTG GCAGCTCCAA GGGATTGGTC 
CGCTTCTACT ACCTGGATGA GCACCGCTCC TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC 
GAGAAAGCCA AGATTTCCAT CGACTCCATC CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA 
ATCTTCCAGC GCTACCCGGA CAGCAGCTTT GACCCCAACT GCTGCTTCAG CATCTACCAT 
GGCAGCCACA GAGAGTCGCT GGACTTGGTC TCACCCAGCA GTGAAGAGGC ACGTACCTGG 
GTCACCGGCC TTCGCTACCT CATGGCTGGC ATCAGCGATG AAGACAGCCT AGCCCGTCGC 
CAGCGTACCA GGGACCAGTG GCTGAAGCAG ACGTTTGATG AGGCTGACAA GAACGGGGAC 
GGCAGCCTGA GCATCAGTGA GGTTCTGCAG CTGCTACATA AACTCAACGT GAACCTGCCC 
CGGCAGAGGG TGAAACAGAT GTTCAGGGAG GCGGACACAG ATGACCATCA GGGGACATTA 
GGCTTTGAGG AATTCTGCGC CTTCTACAAG ATGATGTCTA CCCGCCGAGA CCTCTACCTG 
CTCATGCTGA CCTACAGCAA CCATAAGGAC CACTTGGATG CCTCCGACCT GCAGCGCTTT 
CTGGAGGTGG AGCAGAAGAT GAACGGTGTG ACCCTGGAGA GCTGTCAGAA CATCATTGAG 
CAGTTTGAGC CTTGCCTGGA AAATAAGAGC AAGGGGATGC TGGGGATTGA TGGCTTTACC 
AACTACACCC GGAGCCCCGC CGGTGACATC TTCAACCCTG AGCACAACAG AGTGCACCAA 
GACATGACGC AGCCGCTGAG TCACTACTTC ATCACCTCGT CCCACAACAC CTACCTCGTG 
GGTGACCAGC TCATGTCCCA GTCTCGGGTG GACATGTATG CCTGGGTCCT GCAGGCCGGC 
TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC GGGCCCGATG GAGAACCCAT TGTCCATCAT 
GGCTACACTC TGACCTCCAA GATCCTTTTC AAAGATGTCA TCGAAACCAT CAACAAATAC 
GCCTTCATCA AGAATGAGTA CCCAGTGATC CTGTCCATTG AGAACCACTG TAGTGTTGTT 
CAGCAGAAGA AGATGGCCCA GTATCTGACT GACATCCTCG GGGACAAGCT GGACCTCTCC 
TCAGTGAGCA GCGAAGATGC CACCATGCTT CCATCTCCAC AGATGCTCAA GGGCAAGATC 
CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA 
TCTGATGAGG ACAGTGCTGA TGAGATGGAG GATGACTGCA AGCTCCTCAA TGGGGATGCC 
TCCACCAATC GGAAGCGTGT GGAAAACATT GCAAAGAAGA AGCTGGATTC TCTGATCAAG 
GAGTCAAAGA TCCGTGACTG TGAAGACCCC AATGACTTCT CTGTGTCCAC ACTATCCCCC 
TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC 
GTGGAGGCCG GGGAAGACAG CGGGGTGAGC AGGCAGAATA GCCGCCTCTT CATGAGCAGC 
TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG 
GACGAGACCC TGGACTCCCC AGGAAGCCAG AGCCGAGGGA CTGCCCGGCA AAAGAAGACC 
ATGAAGCTGT CACGAGCCCT CTCGGACCTA GTGAAATATA CCAAGTCTGT GGGGACCCAT 
GACGTGGAGA TAGAGGTGGT ATCTAGCTGG CAGGTGTCGT CCTTCAGTGA GACCAAGGCC 
CATCAGATCC TGCAGCAGAA GCCCACCCAG TACCTGCGCT TCAACCAGCA CCAGCTCTCG 
CGCATATACC CCTCCTCCTA CCGTGTGGAC TCCAGCAACT ACAATCCACA ACCCTTCTGG 
AACGCTGGTT GCCAGATGGT TGCCCTGAAC TACCAGTCAG AGGGGCGGAT GCTACAGCTG 
AACAGGGCCA AGTTCAGCGC CAACGGTGAC TGTGGCTACG TGCTCAAACC CCAGTGCATG 
TGCCAGGGTG TCTTCAACCC CAACTCGGAG GATCCCCTGC CGGGGCAGCT CAAGAAGCAG 
CTGGCCCTGA GGATCATCAG TGGCCAGCAG CTGCCCAAAC CACGGGACTC GGTGCTGGGC 
GACCGTGGGG AGATCATCGA CCCCTTCGTG GAGGTGGAGG TCATTGGGCT CCCCGTGGAC 
TGCAGCAAGG AGCAGACCCG AGTGGTGGAC GACAACGGAT TCAACCCCAT GTGGGAGGAG 
ACACTGGTGT TCACCGTGCA CATGCCAGAG ATTGCGCTTG TACGCTTCCT GGTCTGGGAC 
CATGACCCCA TTGGACGTGA CTTCATCGGC CAGAGGACAC TAGCCTTCAG CAGCATAATG 
CCAGGCTACC GGCATGTGTA CCTAGAAGGG ATGGAAGAGG CCTCTATCTT TGTTCATGTG 
GCTGTCAGTG ACATTAGTGG TAAGGTCAAG CAGACTCTGG GTCTAAAAGG TCTCTTCCTC 
CGAGGCACAA AGCCAGGCTC GCTGGACAGT CATGCTGCTG GACAGCCCCT CCCCCGGCCC 
TCCGTTAGCC AGAGGCTCCT GCGGCGCACG GCCAGTGCCC CGACCAAAAG CCAGAAGCCA 
AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG GGCACACAGG ACGCAGGCTC CGAGGGGGCA 
GCTGATGACG TGGCACCCTC TAGCCCCAAC CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG 
TCAGGCAGCA GCAGCCCCCG AGATACTCGC CTCTTCCCCC TGCAGCGGCC CATCTCCCCC 
CTGTGCAGCC TGGAACCCAT TGCCGAGGAA CCAGCCCTGG GCCCTGGCCT CCCGTTACAG 
GCAGCTGCCC CCACAGGTCC ATCTCAGGAA GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC 
AAAGTGACCA GCTCCCAGCA GACATCTCTG GGAGCTTTTG GAACCCTCCA GCTCAGGATT 
GGGGGCGGTA GGGAAAACGA AGAGCCACCC CTCAGGCCAC ACAATGGTGG GATCTCCAGC 
GGGCCACGTG AGGGGACTTC TGGAAGACAG ACAGACAGCA AGAGCCGCTC CCGGGTCCCG 
GGCCACTTGC CAGTGGTTAG GAGGGCCAAG AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT 
CCCACACCTG CAGTGTACTC GGATGCCACG GGCACTGACA GGTTATGGCA GCGGCTGGAG 
CCTGGGAGCC ACAGGGACAG TGTCTCCTCA TCCTCCAGCA TGTCCTCCAA CGACACCGTC 
ATAGACCTAT CACTGCCCAG CCTCGGCCTC TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC 
TCTCTTGGAC GCCTAACCTC ACGGCCCTGC TTAGCCTCAG CTGCCCGTCC TGACCTGCCT 
CCTGTGACCA AGAGCAAATC GAACCCCAAC CTGCGGGTTG CGGGTGGGCT GCCTACTGCA 
CCCGACGAGC TGCAGCCCCG GCCTCTTGCC CCGCGGCTGA CCGGCCACCA TCCACGGCCA 
CCGTGGCATC ACCTCACCCT GGTGGGCCTG CGGGACTGCC CAGTGTCTGC TAAGTCCAAG 
AGTCTGGGAG ACCTGACAGC TGACGACTTT GCTCCCAGCT TTCAGGGCAG CACCAGCAGC 
CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG GCGCATCAGG TGCTGGAGCC GGGAATACGG 
CGAGATGCTC TCACGGAGCA GCTACGCTGG CTCACCGGCT TCCAGCAGGC TGGGGACATC 
ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG GACGGGTCTG TAGGGGGCCC CAGCTTCCTG 
AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA 
GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC CAGGACTCAC GGGGTCCCCC AGAGGAGGAG 
CGGGGCACCC CTGAGGGTGC CTGTTCTGTA GGCCATGAGG GCTGTGTGGA TGTGCCAATG 
CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( NM_001113360.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001106831.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 2, mRNA.

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CloneID	OMu16963
Clone ID Related Accession (Same CDS sequence)	NM_001113360.3 , NM_001113360.2
Accession Version	NM_001113360.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DQ176851.1, AK044619.1, CJ252783.1 and BG261709.1. On Apr 20, 2010 this sequence version replaced NM_001113360.1. Transcript Variant: This variant (2) encodes the longest isoform (b). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGGAC TGGCCTGGGG ACCATCCAGG GCCGCAGGCA GCAGTTGGGT CAATGCCAGT 
GGGACCTGGG AACAGCCCTT GCGGGGCTTC AGTGGGCTCC AAGGAGGAAG ACGAAGAGGT 
AGAGGAGAGA AGGGGATCCC TGAGGAGCCC CTGTGCCAGC TGACACCTCA GCTTGGCCTG 
TCGCTGAGGG TCCCTTTTGG CTTGGGGGAC TACGGCTTGG ATATGCCTGG TCCCCAGCCG 
TCTGCTGCCA GCCAGACCAC AGGAGCCGTG GCTTGCCTGG CAGAGGTACT CCTCTGGGTT 
GGAGGGAGCG TGGTAGTGTC ACCAAGATGG CAGCTCAGCC TTGTAGTGGA GCGATGCATG 
AGTGCCATGC AAGAGGGGAC CCAGATGGTG AAGCTCCGTG GCAGCTCCAA GGGATTGGTC 
CGCTTCTACT ACCTGGATGA GCACCGCTCC TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC 
GAGAAAGCCA AGATTTCCAT CGACTCCATC CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA 
ATCTTCCAGC GCTACCCGGA CAGCAGCTTT GACCCCAACT GCTGCTTCAG CATCTACCAT 
GGCAGCCACA GAGAGTCGCT GGACTTGGTC TCACCCAGCA GTGAAGAGGC ACGTACCTGG 
GTCACCGGCC TTCGCTACCT CATGGCTGGC ATCAGCGATG AAGACAGCCT AGCCCGTCGC 
CAGCGTACCA GGGACCAGTG GCTGAAGCAG ACGTTTGATG AGGCTGACAA GAACGGGGAC 
GGCAGCCTGA GCATCAGTGA GGTTCTGCAG CTGCTACATA AACTCAACGT GAACCTGCCC 
CGGCAGAGGG TGAAACAGAT GTTCAGGGAG GCGGACACAG ATGACCATCA GGGGACATTA 
GGCTTTGAGG AATTCTGCGC CTTCTACAAG ATGATGTCTA CCCGCCGAGA CCTCTACCTG 
CTCATGCTGA CCTACAGCAA CCATAAGGAC CACTTGGATG CCTCCGACCT GCAGCGCTTT 
CTGGAGGTGG AGCAGAAGAT GAACGGTGTG ACCCTGGAGA GCTGTCAGAA CATCATTGAG 
CAGTTTGAGC CTTGCCTGGA AAATAAGAGC AAGGGGATGC TGGGGATTGA TGGCTTTACC 
AACTACACCC GGAGCCCCGC CGGTGACATC TTCAACCCTG AGCACAACAG AGTGCACCAA 
GACATGACGC AGCCGCTGAG TCACTACTTC ATCACCTCGT CCCACAACAC CTACCTCGTG 
GGTGACCAGC TCATGTCCCA GTCTCGGGTG GACATGTATG CCTGGGTCCT GCAGGCCGGC 
TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC GGGCCCGATG GAGAACCCAT TGTCCATCAT 
GGCTACACTC TGACCTCCAA GATCCTTTTC AAAGATGTCA TCGAAACCAT CAACAAATAC 
GCCTTCATCA AGAATGAGTA CCCAGTGATC CTGTCCATTG AGAACCACTG TAGTGTTGTT 
CAGCAGAAGA AGATGGCCCA GTATCTGACT GACATCCTCG GGGACAAGCT GGACCTCTCC 
TCAGTGAGCA GCGAAGATGC CACCATGCTT CCATCTCCAC AGATGCTCAA GGGCAAGATC 
CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA 
TCTGATGAGG ACAGTGCTGA TGAGATGGAG GATGACTGCA AGCTCCTCAA TGGGGATGCC 
TCCACCAATC GGAAGCGTGT GGAAAACATT GCAAAGAAGA AGCTGGATTC TCTGATCAAG 
GAGTCAAAGA TCCGTGACTG TGAAGACCCC AATGACTTCT CTGTGTCCAC ACTATCCCCC 
TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC 
GTGGAGGCCG GGGAAGACAG CGGGGTGAGC AGGCAGAATA GCCGCCTCTT CATGAGCAGC 
TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG 
GACGAGACCC TGGACTCCCC AGGAAGCCAG AGCCGAGGGA CTGCCCGGCA AAAGAAGACC 
ATGAAGCTGT CACGAGCCCT CTCGGACCTA GTGAAATATA CCAAGTCTGT GGGGACCCAT 
GACGTGGAGA TAGAGGTGGT ATCTAGCTGG CAGGTGTCGT CCTTCAGTGA GACCAAGGCC 
CATCAGATCC TGCAGCAGAA GCCCACCCAG TACCTGCGCT TCAACCAGCA CCAGCTCTCG 
CGCATATACC CCTCCTCCTA CCGTGTGGAC TCCAGCAACT ACAATCCACA ACCCTTCTGG 
AACGCTGGTT GCCAGATGGT TGCCCTGAAC TACCAGTCAG AGGGGCGGAT GCTACAGCTG 
AACAGGGCCA AGTTCAGCGC CAACGGTGAC TGTGGCTACG TGCTCAAACC CCAGTGCATG 
TGCCAGGGTG TCTTCAACCC CAACTCGGAG GATCCCCTGC CGGGGCAGCT CAAGAAGCAG 
CTGGCCCTGA GGATCATCAG TGGCCAGCAG CTGCCCAAAC CACGGGACTC GGTGCTGGGC 
GACCGTGGGG AGATCATCGA CCCCTTCGTG GAGGTGGAGG TCATTGGGCT CCCCGTGGAC 
TGCAGCAAGG AGCAGACCCG AGTGGTGGAC GACAACGGAT TCAACCCCAT GTGGGAGGAG 
ACACTGGTGT TCACCGTGCA CATGCCAGAG ATTGCGCTTG TACGCTTCCT GGTCTGGGAC 
CATGACCCCA TTGGACGTGA CTTCATCGGC CAGAGGACAC TAGCCTTCAG CAGCATAATG 
CCAGGCTACC GGCATGTGTA CCTAGAAGGG ATGGAAGAGG CCTCTATCTT TGTTCATGTG 
GCTGTCAGTG ACATTAGTGG TAAGGTCAAG CAGACTCTGG GTCTAAAAGG TCTCTTCCTC 
CGAGGCACAA AGCCAGGCTC GCTGGACAGT CATGCTGCTG GACAGCCCCT CCCCCGGCCC 
TCCGTTAGCC AGAGGCTCCT GCGGCGCACG GCCAGTGCCC CGACCAAAAG CCAGAAGCCA 
AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG GGCACACAGG ACGCAGGCTC CGAGGGGGCA 
GCTGATGACG TGGCACCCTC TAGCCCCAAC CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG 
TCAGGCAGCA GCAGCCCCCG AGATACTCGC CTCTTCCCCC TGCAGCGGCC CATCTCCCCC 
CTGTGCAGCC TGGAACCCAT TGCCGAGGAA CCAGCCCTGG GCCCTGGCCT CCCGTTACAG 
GCAGCTGCCC CCACAGGTCC ATCTCAGGAA GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC 
AAAGTGACCA GCTCCCAGCA GACATCTCTG GGAGCTTTTG GAACCCTCCA GCTCAGGATT 
GGGGGCGGTA GGGAAAACGA AGAGCCACCC CTCAGGCCAC ACAATGGTGG GATCTCCAGC 
GGGCCACGTG AGGGGACTTC TGGAAGACAG ACAGACAGCA AGAGCCGCTC CCGGGTCCCG 
GGCCACTTGC CAGTGGTTAG GAGGGCCAAG AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT 
CCCACACCTG CAGTGTACTC GGATGCCACG GGCACTGACA GGTTATGGCA GCGGCTGGAG 
CCTGGGAGCC ACAGGGACAG TGTCTCCTCA TCCTCCAGCA TGTCCTCCAA CGACACCGTC 
ATAGACCTAT CACTGCCCAG CCTCGGCCTC TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC 
TCTCTTGGAC GCCTAACCTC ACGGCCCTGC TTAGCCTCAG CTGCCCGTCC TGACCTGCCT 
CCTGTGACCA AGAGCAAATC GAACCCCAAC CTGCGGGTTG CGGGTGGGCT GCCTACTGCA 
CCCGACGAGC TGCAGCCCCG GCCTCTTGCC CCGCGGCTGA CCGGCCACCA TCCACGGCCA 
CCGTGGCATC ACCTCACCCT GGTGGGCCTG CGGGACTGCC CAGTGTCTGC TAAGTCCAAG 
AGTCTGGGAG ACCTGACAGC TGACGACTTT GCTCCCAGCT TTCAGGGCAG CACCAGCAGC 
CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG GCGCATCAGG TGCTGGAGCC GGGAATACGG 
CGAGATGCTC TCACGGAGCA GCTACGCTGG CTCACCGGCT TCCAGCAGGC TGGGGACATC 
ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG GACGGGTCTG TAGGGGGCCC CAGCTTCCTG 
AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA 
GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC CAGGACTCAC GGGGTCCCCC AGAGGAGGAG 
CGGGGCACCC CTGAGGGTGC CTGTTCTGTA GGCCATGAGG GCTGTGTGGA TGTGCCAATG 
CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106831.1
CDS	1..4506
Translation	MGGLAWGPSRAAGSSWVNASGTWEQPLRGFSGLQGGRRRGRGEKGIPEEPLCQLTPQLGLSLRVPFGLGDYGLDMPGPQPSAASQTTGAVACLAEVLLWVGGSVVVSPRWQLSLVVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	NM_001113360.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001113360.2

ORF Insert Sequence:

ATGGGCGGAC TGGCCTGGGG ACCATCCAGG GCCGCAGGCA GCAGTTGGGT CAATGCCAGT 
GGGACCTGGG AACAGCCCTT GCGGGGCTTC AGTGGGCTCC AAGGAGGAAG ACGAAGAGGT 
AGAGGAGAGA AGGGGATCCC TGAGGAGCCC CTGTGCCAGC TGACACCTCA GCTTGGCCTG 
TCGCTGAGGG TCCCTTTTGG CTTGGGGGAC TACGGCTTGG ATATGCCTGG TCCCCAGCCG 
TCTGCTGCCA GCCAGACCAC AGGAGCCGTG GCTTGCCTGG CAGAGGTACT CCTCTGGGTT 
GGAGGGAGCG TGGTAGTGTC ACCAAGATGG CAGCTCAGCC TTGTAGTGGA GCGATGCATG 
AGTGCCATGC AAGAGGGGAC CCAGATGGTG AAGCTCCGTG GCAGCTCCAA GGGATTGGTC 
CGCTTCTACT ACCTGGATGA GCACCGCTCC TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC 
GAGAAAGCCA AGATTTCCAT CGACTCCATC CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA 
ATCTTCCAGC GCTACCCGGA CAGCAGCTTT GACCCCAACT GCTGCTTCAG CATCTACCAT 
GGCAGCCACA GAGAGTCGCT GGACTTGGTC TCACCCAGCA GTGAAGAGGC ACGTACCTGG 
GTCACCGGCC TTCGCTACCT CATGGCTGGC ATCAGCGATG AAGACAGCCT AGCCCGTCGC 
CAGCGTACCA GGGACCAGTG GCTGAAGCAG ACGTTTGATG AGGCTGACAA GAACGGGGAC 
GGCAGCCTGA GCATCAGTGA GGTTCTGCAG CTGCTACATA AACTCAACGT GAACCTGCCC 
CGGCAGAGGG TGAAACAGAT GTTCAGGGAG GCGGACACAG ATGACCATCA GGGGACATTA 
GGCTTTGAGG AATTCTGCGC CTTCTACAAG ATGATGTCTA CCCGCCGAGA CCTCTACCTG 
CTCATGCTGA CCTACAGCAA CCATAAGGAC CACTTGGATG CCTCCGACCT GCAGCGCTTT 
CTGGAGGTGG AGCAGAAGAT GAACGGTGTG ACCCTGGAGA GCTGTCAGAA CATCATTGAG 
CAGTTTGAGC CTTGCCTGGA AAATAAGAGC AAGGGGATGC TGGGGATTGA TGGCTTTACC 
AACTACACCC GGAGCCCCGC CGGTGACATC TTCAACCCTG AGCACAACAG AGTGCACCAA 
GACATGACGC AGCCGCTGAG TCACTACTTC ATCACCTCGT CCCACAACAC CTACCTCGTG 
GGTGACCAGC TCATGTCCCA GTCTCGGGTG GACATGTATG CCTGGGTCCT GCAGGCCGGC 
TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC GGGCCCGATG GAGAACCCAT TGTCCATCAT 
GGCTACACTC TGACCTCCAA GATCCTTTTC AAAGATGTCA TCGAAACCAT CAACAAATAC 
GCCTTCATCA AGAATGAGTA CCCAGTGATC CTGTCCATTG AGAACCACTG TAGTGTTGTT 
CAGCAGAAGA AGATGGCCCA GTATCTGACT GACATCCTCG GGGACAAGCT GGACCTCTCC 
TCAGTGAGCA GCGAAGATGC CACCATGCTT CCATCTCCAC AGATGCTCAA GGGCAAGATC 
CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA 
TCTGATGAGG ACAGTGCTGA TGAGATGGAG GATGACTGCA AGCTCCTCAA TGGGGATGCC 
TCCACCAATC GGAAGCGTGT GGAAAACATT GCAAAGAAGA AGCTGGATTC TCTGATCAAG 
GAGTCAAAGA TCCGTGACTG TGAAGACCCC AATGACTTCT CTGTGTCCAC ACTATCCCCC 
TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC 
GTGGAGGCCG GGGAAGACAG CGGGGTGAGC AGGCAGAATA GCCGCCTCTT CATGAGCAGC 
TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG 
GACGAGACCC TGGACTCCCC AGGAAGCCAG AGCCGAGGGA CTGCCCGGCA AAAGAAGACC 
ATGAAGCTGT CACGAGCCCT CTCGGACCTA GTGAAATATA CCAAGTCTGT GGGGACCCAT 
GACGTGGAGA TAGAGGTGGT ATCTAGCTGG CAGGTGTCGT CCTTCAGTGA GACCAAGGCC 
CATCAGATCC TGCAGCAGAA GCCCACCCAG TACCTGCGCT TCAACCAGCA CCAGCTCTCG 
CGCATATACC CCTCCTCCTA CCGTGTGGAC TCCAGCAACT ACAATCCACA ACCCTTCTGG 
AACGCTGGTT GCCAGATGGT TGCCCTGAAC TACCAGTCAG AGGGGCGGAT GCTACAGCTG 
AACAGGGCCA AGTTCAGCGC CAACGGTGAC TGTGGCTACG TGCTCAAACC CCAGTGCATG 
TGCCAGGGTG TCTTCAACCC CAACTCGGAG GATCCCCTGC CGGGGCAGCT CAAGAAGCAG 
CTGGCCCTGA GGATCATCAG TGGCCAGCAG CTGCCCAAAC CACGGGACTC GGTGCTGGGC 
GACCGTGGGG AGATCATCGA CCCCTTCGTG GAGGTGGAGG TCATTGGGCT CCCCGTGGAC 
TGCAGCAAGG AGCAGACCCG AGTGGTGGAC GACAACGGAT TCAACCCCAT GTGGGAGGAG 
ACACTGGTGT TCACCGTGCA CATGCCAGAG ATTGCGCTTG TACGCTTCCT GGTCTGGGAC 
CATGACCCCA TTGGACGTGA CTTCATCGGC CAGAGGACAC TAGCCTTCAG CAGCATAATG 
CCAGGCTACC GGCATGTGTA CCTAGAAGGG ATGGAAGAGG CCTCTATCTT TGTTCATGTG 
GCTGTCAGTG ACATTAGTGG TAAGGTCAAG CAGACTCTGG GTCTAAAAGG TCTCTTCCTC 
CGAGGCACAA AGCCAGGCTC GCTGGACAGT CATGCTGCTG GACAGCCCCT CCCCCGGCCC 
TCCGTTAGCC AGAGGCTCCT GCGGCGCACG GCCAGTGCCC CGACCAAAAG CCAGAAGCCA 
AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG GGCACACAGG ACGCAGGCTC CGAGGGGGCA 
GCTGATGACG TGGCACCCTC TAGCCCCAAC CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG 
TCAGGCAGCA GCAGCCCCCG AGATACTCGC CTCTTCCCCC TGCAGCGGCC CATCTCCCCC 
CTGTGCAGCC TGGAACCCAT TGCCGAGGAA CCAGCCCTGG GCCCTGGCCT CCCGTTACAG 
GCAGCTGCCC CCACAGGTCC ATCTCAGGAA GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC 
AAAGTGACCA GCTCCCAGCA GACATCTCTG GGAGCTTTTG GAACCCTCCA GCTCAGGATT 
GGGGGCGGTA GGGAAAACGA AGAGCCACCC CTCAGGCCAC ACAATGGTGG GATCTCCAGC 
GGGCCACGTG AGGGGACTTC TGGAAGACAG ACAGACAGCA AGAGCCGCTC CCGGGTCCCG 
GGCCACTTGC CAGTGGTTAG GAGGGCCAAG AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT 
CCCACACCTG CAGTGTACTC GGATGCCACG GGCACTGACA GGTTATGGCA GCGGCTGGAG 
CCTGGGAGCC ACAGGGACAG TGTCTCCTCA TCCTCCAGCA TGTCCTCCAA CGACACCGTC 
ATAGACCTAT CACTGCCCAG CCTCGGCCTC TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC 
TCTCTTGGAC GCCTAACCTC ACGGCCCTGC TTAGCCTCAG CTGCCCGTCC TGACCTGCCT 
CCTGTGACCA AGAGCAAATC GAACCCCAAC CTGCGGGTTG CGGGTGGGCT GCCTACTGCA 
CCCGACGAGC TGCAGCCCCG GCCTCTTGCC CCGCGGCTGA CCGGCCACCA TCCACGGCCA 
CCGTGGCATC ACCTCACCCT GGTGGGCCTG CGGGACTGCC CAGTGTCTGC TAAGTCCAAG 
AGTCTGGGAG ACCTGACAGC TGACGACTTT GCTCCCAGCT TTCAGGGCAG CACCAGCAGC 
CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG GCGCATCAGG TGCTGGAGCC GGGAATACGG 
CGAGATGCTC TCACGGAGCA GCTACGCTGG CTCACCGGCT TCCAGCAGGC TGGGGACATC 
ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG GACGGGTCTG TAGGGGGCCC CAGCTTCCTG 
AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA 
GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC CAGGACTCAC GGGGTCCCCC AGAGGAGGAG 
CGGGGCACCC CTGAGGGTGC CTGTTCTGTA GGCCATGAGG GCTGTGTGGA TGTGCCAATG 
CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250269.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248571.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X3, mRNA.

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CloneID	OMu74424
Clone ID Related Accession (Same CDS sequence)	XM_011250269.2
Accession Version	XM_011250269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTACCT TGCAGGAAGG CTCTGAGGTC CTGTTCTGCA GGGTGAGGTG TTCCTGTCCA 
GCAGCCACTG TTCCTGATTT CAAGTCCAAT CCAGGAGATG TCTGGAGGAG AGTGGAGCGA 
TGCATGAGTG CCATGCAAGA GGGGACCCAG ATGGTGAAGC TCCGTGGCAG CTCCAAGGGA 
TTGGTCCGCT TCTACTACCT GGATGAGCAC CGCTCCTGCC TCCGATGGCG TCCCTCCCGC 
AAGAACGAGA AAGCCAAGAT TTCCATCGAC TCCATCCAGG AGGTGAGTGA GGGCCGGCAG 
TCTGAAATCT TCCAGCGCTA CCCGGACAGC AGCTTTGACC CCAACTGCTG CTTCAGCATC 
TACCATGGCA GCCACAGAGA GTCGCTGGAC TTGGTCTCAC CCAGCAGTGA AGAGGCACGT 
ACCTGGGTCA CCGGCCTTCG CTACCTCATG GCTGGCATCA GCGATGAAGA CAGCCTAGCC 
CGTCGCCAGC GTACCAGGGA CCAGTGGCTG AAGCAGACGT TTGATGAGGC TGACAAGAAC 
GGGGACGGCA GCCTGAGCAT CAGTGAGGTT CTGCAGCTGC TACATAAACT CAACGTGAAC 
CTGCCCCGGC AGAGGGTGAA ACAGATGTTC AGGGAGGCGG ACACAGATGA CCATCAGGGG 
ACATTAGGCT TTGAGGAATT CTGCGCCTTC TACAAGATGA TGTCTACCCG CCGAGACCTC 
TACCTGCTCA TGCTGACCTA CAGCAACCAT AAGGACCACT TGGATGCCTC CGACCTGCAG 
CGCTTTCTGG AGGTGGAGCA GAAGATGAAC GGTGTGACCC TGGAGAGCTG TCAGAACATC 
ATTGAGCAGT TTGAGCCTTG CCTGGAAAAT AAGAGCAAGG GGATGCTGGG GATTGATGGT 
AGTCGTGAGC CGAGCCCAGT GATGGAGATA CACCACGGCG CTCCTGTGTA CACCGCAGGC 
TTTACCAACT ACACCCGGAG CCCCGCCGGT GACATCTTCA ACCCTGAGCA CAACAGAGTG 
CACCAAGACA TGACGCAGCC GCTGAGTCAC TACTTCATCA CCTCGTCCCA CAACACCTAC 
CTCGTGGGTG ACCAGCTCAT GTCCCAGTCT CGGGTGGACA TGTATGCCTG GGTCCTGCAG 
GCCGGCTGCC GCTGTGTGGA GGTGGACTGC TGGGACGGGC CCGATGGAGA ACCCATTGTC 
CATCATGGCT ACACTCTGAC CTCCAAGATC CTTTTCAAAG ATGTCATCGA AACCATCAAC 
AAATACGCCT TCATCAAGAA TGAGTACCCA GTGATCCTGT CCATTGAGAA CCACTGTAGT 
GTTGTTCAGC AGAAGAAGAT GGCCCAGTAT CTGACTGACA TCCTCGGGGA CAAGCTGGAC 
CTCTCCTCAG TGAGCAGCGA AGATGCCACC ATGCTTCCAT CTCCACAGAT GCTCAAGGGC 
AAGATCCTGG TGAAGGGGAA GAAGCTCCCT GCCAATATCA GTGAGGATGC AGAAGAAGGC 
GAAGTATCTG ATGAGGACAG TGCTGATGAG ATGGAGGATG ACTGCAAGCT CCTCAATGGG 
GATGCCTCCA CCAATCGGAA GCGTGTGGAA AACATTGCAA AGAAGAAGCT GGATTCTCTG 
ATCAAGGAGT CAAAGATCCG TGACTGTGAA GACCCCAATG ACTTCTCTGT GTCCACACTA 
TCCCCCTCTG GAAAGCTTGG GCGCAAAGCA GAGGCCAAGA AGGGTCAGAG CAAGGTTGAG 
GAAGACGTGG AGGCCGGGGA AGACAGCGGG GTGAGCAGGC AGAATAGCCG CCTCTTCATG 
AGCAGCTTCT CCAAGCGCAA GAAAAAAGGC AGCAAGATAA AGAAGGTGGC CAGCGTGGAA 
GAGGGGGACG AGACCCTGGA CTCCCCAGGA AGCCAGAGCC GAGGGACTGC CCGGCAAAAG 
AAGACCATGA AGCTGTCACG AGCCCTCTCG GACCTAGTGA AATATACCAA GTCTGTGGGG 
ACCCATGACG TGGAGATAGA GGTGGTATCT AGCTGGCAGG TGTCGTCCTT CAGTGAGACC 
AAGGCCCATC AGATCCTGCA GCAGAAGCCC ACCCAGTACC TGCGCTTCAA CCAGCACCAG 
CTCTCGCGCA TATACCCCTC CTCCTACCGT GTGGACTCCA GCAACTACAA TCCACAACCC 
TTCTGGAACG CTGGTTGCCA GATGGTTGCC CTGAACTACC AGTCAGAGGG GCGGATGCTA 
CAGCTGAACA GGGCCAAGTT CAGCGCCAAC GGTGACTGTG GCTACGTGCT CAAACCCCAG 
TGCATGTGCC AGGGTGTCTT CAACCCCAAC TCGGAGGATC CCCTGCCGGG GCAGCTCAAG 
AAGCAGCTGG CCCTGAGGAT CATCAGTGGC CAGCAGCTGC CCAAACCACG GGACTCGGTG 
CTGGGCGACC GTGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT TGGGCTCCCC 
GTGGACTGCA GCAAGGAGCA GACCCGAGTG GTGGACGACA ACGGATTCAA CCCCATGTGG 
GAGGAGACAC TGGTGTTCAC CGTGCACATG CCAGAGATTG CGCTTGTACG CTTCCTGGTC 
TGGGACCATG ACCCCATTGG ACGTGACTTC ATCGGCCAGA GGACACTAGC CTTCAGCAGC 
ATAATGCCAG GCTACCGGCA TGTGTACCTA GAAGGGATGG AAGAGGCCTC TATCTTTGTT 
CATGTGGCTG TCAGTGACAT TAGTGGTAAG GTCAAGCAGA CTCTGGGTCT AAAAGGTCTC 
TTCCTCCGAG GCACAAAGCC AGGCTCGCTG GACAGTCATG CTGCTGGACA GCCCCTCCCC 
CGGCCCTCCG TTAGCCAGAG GCTCCTGCGG CGCACGGCCA GTGCCCCGAC CAAAAGCCAG 
AAGCCAAGTC GCAAGGGCTT CCCAGAGCTG GCCCTGGGCA CACAGGACGC AGGCTCCGAG 
GGGGCAGCTG ATGACGTGGC ACCCTCTAGC CCCAACCCAG CTCTGGAGGC CCCTACTCAA 
GAGAGGTCAG GCAGCAGCAG CCCCCGAGGT AAGGCACCAG GAGGAGAAGC AACAGAAGAG 
AGGACACTGG CACAGGTGCG GTCCCCAAAT GCCCCCGAAG GCCCTGGACC TGCAGGGATG 
GCCGCCACAT GCATGAAGTG TGTGGTGGGC TCCTGCGCCG GTATGGACGT TGAGGGCCTT 
CAAAGGGAGC AACAACCCAG CCCTGGGCCT GCAGGCAGCC ACATGGCCAT CAGCCATCAG 
CCCAGGGCCC GGGTAGATTC ACTGGGGGGC CCTTGCTGCA GCCCAAGTCC TCGAGCCACC 
CCAGGGAGAA GCAAAGAGGC CCCCAAGGGT CCTAGGGCTC GGCGCCAGGG TCCAGGCGGC 
GGCTCTGTGT CCTCGGACTC CAGCAGCCCA GACAGCCCAG GCAGCCCCAA GGTGGCCCCC 
TGCCAGCCGG AGGGTGCTCA CAGGCAGCAG GGGGCGCTGC AGGGAGAGAT GAACGCCTTG 
TTCGTTCAAA AGCTGGAGGA GATCAGGAGT CATTCCCCCA TGTTCTCCAC CGATACTCGC 
CTCTTCCCCC TGCAGCGGCC CATCTCCCCC CTGTGCAGCC TGGAACCCAT TGCCGAGGAA 
CCAGCCCTGG GCCCTGGCCT CCCGTTACAG GCAGCTGCCC CCACAGGTCC ATCTCAGGAA 
GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC AAAGTGACCA GCTCCCAGCA GACATCTCTG 
GGAGCTTTTG GAACCCTCCA GCTCAGGATT GGGGGCGGTA GGGAAAACGA AGAGCCACCC 
CTCAGGCCAC ACAATGGTGG GATCTCCAGC GGGCCACGTG AGGGGACTTC TGGAAGACAG 
ACAGACAGCA AGAGCCGCTC CCGGGTCCCG GGCCACTTGC CAGTGGTTAG GAGGGCCAAG 
AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT CCCACACCTG CAGTGTACTC GGATGCCACG 
GGCACTGACA GGTTATGGCA GCGGCTGGAG CCTGGGAGCC ACAGGGACAG TGTCTCCTCA 
TCCTCCAGCA TGTCCTCCAA CGACACCGTC ATAGACCTAT CACTGCCCAG CCTCGGCCTC 
TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC TCTCTTGGAC GCCTAACCTC ACGGCCCTGC 
TTAGCCTCAG CTGCCCGTCC TGACCTGCCT CCTGTGACCA AGAGCAAATC GAACCCCAAC 
CTGCGGGTTG CGGGTGGGCT GCCTACTGCA CCCGACGAGC TGCAGCCCCG GCCTCTTGCC 
CCGCGGCTGA CCGGCCACCA TCCACGGCCA CCGTGGCATC ACCTCACCCT GGTGGGCCTG 
CGGGACTGCC CAGTGTCTGC TAAGTCCAAG AGTCTGGGAG ACCTGACAGC TGACGACTTT 
GCTCCCAGCT TTCAGGGCAG CACCAGCAGC CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG 
GCGCATCAGG TGCTGGAGCC GGGAATACGG CGAGATGCTC TCACGGAGCA GCTACGCTGG 
CTCACCGGCT TCCAGCAGGC TGGGGACATC ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG 
GACGGGTCTG TAGGGGGCCC CAGCTTCCTG AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC 
GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC 
CAGGACTCAC GGGGTCCCCC AGAGGAGGAG CGGGGCACCC CTGAGGGTGC CTGTTCTGTA 
GGCCATGAGG GCTGTGTGGA TGTGCCAATG CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT 
GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248571.1
CDS	317..5152
Translation	MPTLQEGSEVLFCRVRCSCPAATVPDFKSNPGDVWRRVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	XM_011250269.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250269.2

ORF Insert Sequence:

ATGCCTACCT TGCAGGAAGG CTCTGAGGTC CTGTTCTGCA GGGTGAGGTG TTCCTGTCCA 
GCAGCCACTG TTCCTGATTT CAAGTCCAAT CCAGGAGATG TCTGGAGGAG AGTGGAGCGA 
TGCATGAGTG CCATGCAAGA GGGGACCCAG ATGGTGAAGC TCCGTGGCAG CTCCAAGGGA 
TTGGTCCGCT TCTACTACCT GGATGAGCAC CGCTCCTGCC TCCGATGGCG TCCCTCCCGC 
AAGAACGAGA AAGCCAAGAT TTCCATCGAC TCCATCCAGG AGGTGAGTGA GGGCCGGCAG 
TCTGAAATCT TCCAGCGCTA CCCGGACAGC AGCTTTGACC CCAACTGCTG CTTCAGCATC 
TACCATGGCA GCCACAGAGA GTCGCTGGAC TTGGTCTCAC CCAGCAGTGA AGAGGCACGT 
ACCTGGGTCA CCGGCCTTCG CTACCTCATG GCTGGCATCA GCGATGAAGA CAGCCTAGCC 
CGTCGCCAGC GTACCAGGGA CCAGTGGCTG AAGCAGACGT TTGATGAGGC TGACAAGAAC 
GGGGACGGCA GCCTGAGCAT CAGTGAGGTT CTGCAGCTGC TACATAAACT CAACGTGAAC 
CTGCCCCGGC AGAGGGTGAA ACAGATGTTC AGGGAGGCGG ACACAGATGA CCATCAGGGG 
ACATTAGGCT TTGAGGAATT CTGCGCCTTC TACAAGATGA TGTCTACCCG CCGAGACCTC 
TACCTGCTCA TGCTGACCTA CAGCAACCAT AAGGACCACT TGGATGCCTC CGACCTGCAG 
CGCTTTCTGG AGGTGGAGCA GAAGATGAAC GGTGTGACCC TGGAGAGCTG TCAGAACATC 
ATTGAGCAGT TTGAGCCTTG CCTGGAAAAT AAGAGCAAGG GGATGCTGGG GATTGATGGT 
AGTCGTGAGC CGAGCCCAGT GATGGAGATA CACCACGGCG CTCCTGTGTA CACCGCAGGC 
TTTACCAACT ACACCCGGAG CCCCGCCGGT GACATCTTCA ACCCTGAGCA CAACAGAGTG 
CACCAAGACA TGACGCAGCC GCTGAGTCAC TACTTCATCA CCTCGTCCCA CAACACCTAC 
CTCGTGGGTG ACCAGCTCAT GTCCCAGTCT CGGGTGGACA TGTATGCCTG GGTCCTGCAG 
GCCGGCTGCC GCTGTGTGGA GGTGGACTGC TGGGACGGGC CCGATGGAGA ACCCATTGTC 
CATCATGGCT ACACTCTGAC CTCCAAGATC CTTTTCAAAG ATGTCATCGA AACCATCAAC 
AAATACGCCT TCATCAAGAA TGAGTACCCA GTGATCCTGT CCATTGAGAA CCACTGTAGT 
GTTGTTCAGC AGAAGAAGAT GGCCCAGTAT CTGACTGACA TCCTCGGGGA CAAGCTGGAC 
CTCTCCTCAG TGAGCAGCGA AGATGCCACC ATGCTTCCAT CTCCACAGAT GCTCAAGGGC 
AAGATCCTGG TGAAGGGGAA GAAGCTCCCT GCCAATATCA GTGAGGATGC AGAAGAAGGC 
GAAGTATCTG ATGAGGACAG TGCTGATGAG ATGGAGGATG ACTGCAAGCT CCTCAATGGG 
GATGCCTCCA CCAATCGGAA GCGTGTGGAA AACATTGCAA AGAAGAAGCT GGATTCTCTG 
ATCAAGGAGT CAAAGATCCG TGACTGTGAA GACCCCAATG ACTTCTCTGT GTCCACACTA 
TCCCCCTCTG GAAAGCTTGG GCGCAAAGCA GAGGCCAAGA AGGGTCAGAG CAAGGTTGAG 
GAAGACGTGG AGGCCGGGGA AGACAGCGGG GTGAGCAGGC AGAATAGCCG CCTCTTCATG 
AGCAGCTTCT CCAAGCGCAA GAAAAAAGGC AGCAAGATAA AGAAGGTGGC CAGCGTGGAA 
GAGGGGGACG AGACCCTGGA CTCCCCAGGA AGCCAGAGCC GAGGGACTGC CCGGCAAAAG 
AAGACCATGA AGCTGTCACG AGCCCTCTCG GACCTAGTGA AATATACCAA GTCTGTGGGG 
ACCCATGACG TGGAGATAGA GGTGGTATCT AGCTGGCAGG TGTCGTCCTT CAGTGAGACC 
AAGGCCCATC AGATCCTGCA GCAGAAGCCC ACCCAGTACC TGCGCTTCAA CCAGCACCAG 
CTCTCGCGCA TATACCCCTC CTCCTACCGT GTGGACTCCA GCAACTACAA TCCACAACCC 
TTCTGGAACG CTGGTTGCCA GATGGTTGCC CTGAACTACC AGTCAGAGGG GCGGATGCTA 
CAGCTGAACA GGGCCAAGTT CAGCGCCAAC GGTGACTGTG GCTACGTGCT CAAACCCCAG 
TGCATGTGCC AGGGTGTCTT CAACCCCAAC TCGGAGGATC CCCTGCCGGG GCAGCTCAAG 
AAGCAGCTGG CCCTGAGGAT CATCAGTGGC CAGCAGCTGC CCAAACCACG GGACTCGGTG 
CTGGGCGACC GTGGGGAGAT CATCGACCCC TTCGTGGAGG TGGAGGTCAT TGGGCTCCCC 
GTGGACTGCA GCAAGGAGCA GACCCGAGTG GTGGACGACA ACGGATTCAA CCCCATGTGG 
GAGGAGACAC TGGTGTTCAC CGTGCACATG CCAGAGATTG CGCTTGTACG CTTCCTGGTC 
TGGGACCATG ACCCCATTGG ACGTGACTTC ATCGGCCAGA GGACACTAGC CTTCAGCAGC 
ATAATGCCAG GCTACCGGCA TGTGTACCTA GAAGGGATGG AAGAGGCCTC TATCTTTGTT 
CATGTGGCTG TCAGTGACAT TAGTGGTAAG GTCAAGCAGA CTCTGGGTCT AAAAGGTCTC 
TTCCTCCGAG GCACAAAGCC AGGCTCGCTG GACAGTCATG CTGCTGGACA GCCCCTCCCC 
CGGCCCTCCG TTAGCCAGAG GCTCCTGCGG CGCACGGCCA GTGCCCCGAC CAAAAGCCAG 
AAGCCAAGTC GCAAGGGCTT CCCAGAGCTG GCCCTGGGCA CACAGGACGC AGGCTCCGAG 
GGGGCAGCTG ATGACGTGGC ACCCTCTAGC CCCAACCCAG CTCTGGAGGC CCCTACTCAA 
GAGAGGTCAG GCAGCAGCAG CCCCCGAGGT AAGGCACCAG GAGGAGAAGC AACAGAAGAG 
AGGACACTGG CACAGGTGCG GTCCCCAAAT GCCCCCGAAG GCCCTGGACC TGCAGGGATG 
GCCGCCACAT GCATGAAGTG TGTGGTGGGC TCCTGCGCCG GTATGGACGT TGAGGGCCTT 
CAAAGGGAGC AACAACCCAG CCCTGGGCCT GCAGGCAGCC ACATGGCCAT CAGCCATCAG 
CCCAGGGCCC GGGTAGATTC ACTGGGGGGC CCTTGCTGCA GCCCAAGTCC TCGAGCCACC 
CCAGGGAGAA GCAAAGAGGC CCCCAAGGGT CCTAGGGCTC GGCGCCAGGG TCCAGGCGGC 
GGCTCTGTGT CCTCGGACTC CAGCAGCCCA GACAGCCCAG GCAGCCCCAA GGTGGCCCCC 
TGCCAGCCGG AGGGTGCTCA CAGGCAGCAG GGGGCGCTGC AGGGAGAGAT GAACGCCTTG 
TTCGTTCAAA AGCTGGAGGA GATCAGGAGT CATTCCCCCA TGTTCTCCAC CGATACTCGC 
CTCTTCCCCC TGCAGCGGCC CATCTCCCCC CTGTGCAGCC TGGAACCCAT TGCCGAGGAA 
CCAGCCCTGG GCCCTGGCCT CCCGTTACAG GCAGCTGCCC CCACAGGTCC ATCTCAGGAA 
GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC AAAGTGACCA GCTCCCAGCA GACATCTCTG 
GGAGCTTTTG GAACCCTCCA GCTCAGGATT GGGGGCGGTA GGGAAAACGA AGAGCCACCC 
CTCAGGCCAC ACAATGGTGG GATCTCCAGC GGGCCACGTG AGGGGACTTC TGGAAGACAG 
ACAGACAGCA AGAGCCGCTC CCGGGTCCCG GGCCACTTGC CAGTGGTTAG GAGGGCCAAG 
AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT CCCACACCTG CAGTGTACTC GGATGCCACG 
GGCACTGACA GGTTATGGCA GCGGCTGGAG CCTGGGAGCC ACAGGGACAG TGTCTCCTCA 
TCCTCCAGCA TGTCCTCCAA CGACACCGTC ATAGACCTAT CACTGCCCAG CCTCGGCCTC 
TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC TCTCTTGGAC GCCTAACCTC ACGGCCCTGC 
TTAGCCTCAG CTGCCCGTCC TGACCTGCCT CCTGTGACCA AGAGCAAATC GAACCCCAAC 
CTGCGGGTTG CGGGTGGGCT GCCTACTGCA CCCGACGAGC TGCAGCCCCG GCCTCTTGCC 
CCGCGGCTGA CCGGCCACCA TCCACGGCCA CCGTGGCATC ACCTCACCCT GGTGGGCCTG 
CGGGACTGCC CAGTGTCTGC TAAGTCCAAG AGTCTGGGAG ACCTGACAGC TGACGACTTT 
GCTCCCAGCT TTCAGGGCAG CACCAGCAGC CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG 
GCGCATCAGG TGCTGGAGCC GGGAATACGG CGAGATGCTC TCACGGAGCA GCTACGCTGG 
CTCACCGGCT TCCAGCAGGC TGGGGACATC ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG 
GACGGGTCTG TAGGGGGCCC CAGCTTCCTG AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC 
GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC 
CAGGACTCAC GGGGTCCCCC AGAGGAGGAG CGGGGCACCC CTGAGGGTGC CTGTTCTGTA 
GGCCATGAGG GCTGTGTGGA TGTGCCAATG CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT 
GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250271.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248573.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X5, mRNA.

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CloneID	OMu74425
Clone ID Related Accession (Same CDS sequence)	XM_011250271.2 , XM_011250271.3 , XM_011250270.1
Accession Version	XM_011250271.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250271.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGCT CCATTGGTCC CGCTTCATTG CCTGAGGACC CAGGGCCGGA TCTAGGCAGC 
AGATGGCTTT TCCTGAGTGC CAACGTTTTG CCTGTGGTGG AGCGATGCAT GAGTGCCATG 
CAAGAGGGGA CCCAGATGGT GAAGCTCCGT GGCAGCTCCA AGGGATTGGT CCGCTTCTAC 
TACCTGGATG AGCACCGCTC CTGCCTCCGA TGGCGTCCCT CCCGCAAGAA CGAGAAAGCC 
AAGATTTCCA TCGACTCCAT CCAGGAGGTG AGTGAGGGCC GGCAGTCTGA AATCTTCCAG 
CGCTACCCGG ACAGCAGCTT TGACCCCAAC TGCTGCTTCA GCATCTACCA TGGCAGCCAC 
AGAGAGTCGC TGGACTTGGT CTCACCCAGC AGTGAAGAGG CACGTACCTG GGTCACCGGC 
CTTCGCTACC TCATGGCTGG CATCAGCGAT GAAGACAGCC TAGCCCGTCG CCAGCGTACC 
AGGGACCAGT GGCTGAAGCA GACGTTTGAT GAGGCTGACA AGAACGGGGA CGGCAGCCTG 
AGCATCAGTG AGGTTCTGCA GCTGCTACAT AAACTCAACG TGAACCTGCC CCGGCAGAGG 
GTGAAACAGA TGTTCAGGGA GGCGGACACA GATGACCATC AGGGGACATT AGGCTTTGAG 
GAATTCTGCG CCTTCTACAA GATGATGTCT ACCCGCCGAG ACCTCTACCT GCTCATGCTG 
ACCTACAGCA ACCATAAGGA CCACTTGGAT GCCTCCGACC TGCAGCGCTT TCTGGAGGTG 
GAGCAGAAGA TGAACGGTGT GACCCTGGAG AGCTGTCAGA ACATCATTGA GCAGTTTGAG 
CCTTGCCTGG AAAATAAGAG CAAGGGGATG CTGGGGATTG ATGGTAGTCG TGAGCCGAGC 
CCAGTGATGG AGATACACCA CGGCGCTCCT GTGTACACCG CAGGCTTTAC CAACTACACC 
CGGAGCCCCG CCGGTGACAT CTTCAACCCT GAGCACAACA GAGTGCACCA AGACATGACG 
CAGCCGCTGA GTCACTACTT CATCACCTCG TCCCACAACA CCTACCTCGT GGGTGACCAG 
CTCATGTCCC AGTCTCGGGT GGACATGTAT GCCTGGGTCC TGCAGGCCGG CTGCCGCTGT 
GTGGAGGTGG ACTGCTGGGA CGGGCCCGAT GGAGAACCCA TTGTCCATCA TGGCTACACT 
CTGACCTCCA AGATCCTTTT CAAAGATGTC ATCGAAACCA TCAACAAATA CGCCTTCATC 
AAGAATGAGT ACCCAGTGAT CCTGTCCATT GAGAACCACT GTAGTGTTGT TCAGCAGAAG 
AAGATGGCCC AGTATCTGAC TGACATCCTC GGGGACAAGC TGGACCTCTC CTCAGTGAGC 
AGCGAAGATG CCACCATGCT TCCATCTCCA CAGATGCTCA AGGGCAAGAT CCTGGTGAAG 
GGGAAGAAGC TCCCTGCCAA TATCAGTGAG GATGCAGAAG AAGGCGAAGT ATCTGATGAG 
GACAGTGCTG ATGAGATGGA GGATGACTGC AAGCTCCTCA ATGGGGATGC CTCCACCAAT 
CGGAAGCGTG TGGAAAACAT TGCAAAGAAG AAGCTGGATT CTCTGATCAA GGAGTCAAAG 
ATCCGTGACT GTGAAGACCC CAATGACTTC TCTGTGTCCA CACTATCCCC CTCTGGAAAG 
CTTGGGCGCA AAGCAGAGGC CAAGAAGGGT CAGAGCAAGG TTGAGGAAGA CGTGGAGGCC 
GGGGAAGACA GCGGGGTGAG CAGGCAGAAT AGCCGCCTCT TCATGAGCAG CTTCTCCAAG 
CGCAAGAAAA AAGGCAGCAA GATAAAGAAG GTGGCCAGCG TGGAAGAGGG GGACGAGACC 
CTGGACTCCC CAGGAAGCCA GAGCCGAGGG ACTGCCCGGC AAAAGAAGAC CATGAAGCTG 
TCACGAGCCC TCTCGGACCT AGTGAAATAT ACCAAGTCTG TGGGGACCCA TGACGTGGAG 
ATAGAGGTGG TATCTAGCTG GCAGGTGTCG TCCTTCAGTG AGACCAAGGC CCATCAGATC 
CTGCAGCAGA AGCCCACCCA GTACCTGCGC TTCAACCAGC ACCAGCTCTC GCGCATATAC 
CCCTCCTCCT ACCGTGTGGA CTCCAGCAAC TACAATCCAC AACCCTTCTG GAACGCTGGT 
TGCCAGATGG TTGCCCTGAA CTACCAGTCA GAGGGGCGGA TGCTACAGCT GAACAGGGCC 
AAGTTCAGCG CCAACGGTGA CTGTGGCTAC GTGCTCAAAC CCCAGTGCAT GTGCCAGGGT 
GTCTTCAACC CCAACTCGGA GGATCCCCTG CCGGGGCAGC TCAAGAAGCA GCTGGCCCTG 
AGGATCATCA GTGGCCAGCA GCTGCCCAAA CCACGGGACT CGGTGCTGGG CGACCGTGGG 
GAGATCATCG ACCCCTTCGT GGAGGTGGAG GTCATTGGGC TCCCCGTGGA CTGCAGCAAG 
GAGCAGACCC GAGTGGTGGA CGACAACGGA TTCAACCCCA TGTGGGAGGA GACACTGGTG 
TTCACCGTGC ACATGCCAGA GATTGCGCTT GTACGCTTCC TGGTCTGGGA CCATGACCCC 
ATTGGACGTG ACTTCATCGG CCAGAGGACA CTAGCCTTCA GCAGCATAAT GCCAGGCTAC 
CGGCATGTGT ACCTAGAAGG GATGGAAGAG GCCTCTATCT TTGTTCATGT GGCTGTCAGT 
GACATTAGTG GTAAGGTCAA GCAGACTCTG GGTCTAAAAG GTCTCTTCCT CCGAGGCACA 
AAGCCAGGCT CGCTGGACAG TCATGCTGCT GGACAGCCCC TCCCCCGGCC CTCCGTTAGC 
CAGAGGCTCC TGCGGCGCAC GGCCAGTGCC CCGACCAAAA GCCAGAAGCC AAGTCGCAAG 
GGCTTCCCAG AGCTGGCCCT GGGCACACAG GACGCAGGCT CCGAGGGGGC AGCTGATGAC 
GTGGCACCCT CTAGCCCCAA CCCAGCTCTG GAGGCCCCTA CTCAAGAGAG GTCAGGCAGC 
AGCAGCCCCC GAGGTAAGGC ACCAGGAGGA GAAGCAACAG AAGAGAGGAC ACTGGCACAG 
GTGCGGTCCC CAAATGCCCC CGAAGGCCCT GGACCTGCAG GGATGGCCGC CACATGCATG 
AAGTGTGTGG TGGGCTCCTG CGCCGGTATG GACGTTGAGG GCCTTCAAAG GGAGCAACAA 
CCCAGCCCTG GGCCTGCAGG CAGCCACATG GCCATCAGCC ATCAGCCCAG GGCCCGGGTA 
GATTCACTGG GGGGCCCTTG CTGCAGCCCA AGTCCTCGAG CCACCCCAGG GAGAAGCAAA 
GAGGCCCCCA AGGGTCCTAG GGCTCGGCGC CAGGGTCCAG GCGGCGGCTC TGTGTCCTCG 
GACTCCAGCA GCCCAGACAG CCCAGGCAGC CCCAAGGTGG CCCCCTGCCA GCCGGAGGGT 
GCTCACAGGC AGCAGGGGGC GCTGCAGGGA GAGATGAACG CCTTGTTCGT TCAAAAGCTG 
GAGGAGATCA GGAGTCATTC CCCCATGTTC TCCACCGATA CTCGCCTCTT CCCCCTGCAG 
CGGCCCATCT CCCCCCTGTG CAGCCTGGAA CCCATTGCCG AGGAACCAGC CCTGGGCCCT 
GGCCTCCCGT TACAGGCAGC TGCCCCCACA GGTCCATCTC AGGAAGGGTC CCAGTGCCCT 
GTAGGCCTGG GAGCCAAAGT GACCAGCTCC CAGCAGACAT CTCTGGGAGC TTTTGGAACC 
CTCCAGCTCA GGATTGGGGG CGGTAGGGAA AACGAAGAGC CACCCCTCAG GCCACACAAT 
GGTGGGATCT CCAGCGGGCC ACGTGAGGGG ACTTCTGGAA GACAGACAGA CAGCAAGAGC 
CGCTCCCGGG TCCCGGGCCA CTTGCCAGTG GTTAGGAGGG CCAAGAGTGA GGGCCAGGTG 
CTCTCGGAGC TCTCTCCCAC ACCTGCAGTG TACTCGGATG CCACGGGCAC TGACAGGTTA 
TGGCAGCGGC TGGAGCCTGG GAGCCACAGG GACAGTGTCT CCTCATCCTC CAGCATGTCC 
TCCAACGACA CCGTCATAGA CCTATCACTG CCCAGCCTCG GCCTCTGCCG CAGCCGAGAG 
AGCATTCCAG GGGTCTCTCT TGGACGCCTA ACCTCACGGC CCTGCTTAGC CTCAGCTGCC 
CGTCCTGACC TGCCTCCTGT GACCAAGAGC AAATCGAACC CCAACCTGCG GGTTGCGGGT 
GGGCTGCCTA CTGCACCCGA CGAGCTGCAG CCCCGGCCTC TTGCCCCGCG GCTGACCGGC 
CACCATCCAC GGCCACCGTG GCATCACCTC ACCCTGGTGG GCCTGCGGGA CTGCCCAGTG 
TCTGCTAAGT CCAAGAGTCT GGGAGACCTG ACAGCTGACG ACTTTGCTCC CAGCTTTCAG 
GGCAGCACCA GCAGCCTGAG CTGCGGCCTA GGCTCCCTGG GTGTGGCGCA TCAGGTGCTG 
GAGCCGGGAA TACGGCGAGA TGCTCTCACG GAGCAGCTAC GCTGGCTCAC CGGCTTCCAG 
CAGGCTGGGG ACATCACGTC GCCCACCAGC TTGGGCCCAG CTGGGGACGG GTCTGTAGGG 
GGCCCCAGCT TCCTGAGGCG TTCCTCCTCT CGGAGCCAGA GCCGCGTGCG CGCTATTGCC 
AGCCGAGCCC GCCAAGCGCA GGAGCGCCAG CAGAGGCTGA GAGGCCAGGA CTCACGGGGT 
CCCCCAGAGG AGGAGCGGGG CACCCCTGAG GGTGCCTGTT CTGTAGGCCA TGAGGGCTGT 
GTGGATGTGC CAATGCCTGC CAAGGGAGCC CCTGAGCAGG TGTGTGGTGC TGCTGATGGC 
CAGCTGCTGC TCAGGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248573.1
CDS	353..5173
Translation	MDSSIGPASLPEDPGPDLGSRWLFLSANVLPVVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	XM_011250271.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250271.2

ORF Insert Sequence:

ATGGATAGCT CCATTGGTCC CGCTTCATTG CCTGAGGACC CAGGGCCGGA TCTAGGCAGC 
AGATGGCTTT TCCTGAGTGC CAACGTTTTG CCTGTGGTGG AGCGATGCAT GAGTGCCATG 
CAAGAGGGGA CCCAGATGGT GAAGCTCCGT GGCAGCTCCA AGGGATTGGT CCGCTTCTAC 
TACCTGGATG AGCACCGCTC CTGCCTCCGA TGGCGTCCCT CCCGCAAGAA CGAGAAAGCC 
AAGATTTCCA TCGACTCCAT CCAGGAGGTG AGTGAGGGCC GGCAGTCTGA AATCTTCCAG 
CGCTACCCGG ACAGCAGCTT TGACCCCAAC TGCTGCTTCA GCATCTACCA TGGCAGCCAC 
AGAGAGTCGC TGGACTTGGT CTCACCCAGC AGTGAAGAGG CACGTACCTG GGTCACCGGC 
CTTCGCTACC TCATGGCTGG CATCAGCGAT GAAGACAGCC TAGCCCGTCG CCAGCGTACC 
AGGGACCAGT GGCTGAAGCA GACGTTTGAT GAGGCTGACA AGAACGGGGA CGGCAGCCTG 
AGCATCAGTG AGGTTCTGCA GCTGCTACAT AAACTCAACG TGAACCTGCC CCGGCAGAGG 
GTGAAACAGA TGTTCAGGGA GGCGGACACA GATGACCATC AGGGGACATT AGGCTTTGAG 
GAATTCTGCG CCTTCTACAA GATGATGTCT ACCCGCCGAG ACCTCTACCT GCTCATGCTG 
ACCTACAGCA ACCATAAGGA CCACTTGGAT GCCTCCGACC TGCAGCGCTT TCTGGAGGTG 
GAGCAGAAGA TGAACGGTGT GACCCTGGAG AGCTGTCAGA ACATCATTGA GCAGTTTGAG 
CCTTGCCTGG AAAATAAGAG CAAGGGGATG CTGGGGATTG ATGGTAGTCG TGAGCCGAGC 
CCAGTGATGG AGATACACCA CGGCGCTCCT GTGTACACCG CAGGCTTTAC CAACTACACC 
CGGAGCCCCG CCGGTGACAT CTTCAACCCT GAGCACAACA GAGTGCACCA AGACATGACG 
CAGCCGCTGA GTCACTACTT CATCACCTCG TCCCACAACA CCTACCTCGT GGGTGACCAG 
CTCATGTCCC AGTCTCGGGT GGACATGTAT GCCTGGGTCC TGCAGGCCGG CTGCCGCTGT 
GTGGAGGTGG ACTGCTGGGA CGGGCCCGAT GGAGAACCCA TTGTCCATCA TGGCTACACT 
CTGACCTCCA AGATCCTTTT CAAAGATGTC ATCGAAACCA TCAACAAATA CGCCTTCATC 
AAGAATGAGT ACCCAGTGAT CCTGTCCATT GAGAACCACT GTAGTGTTGT TCAGCAGAAG 
AAGATGGCCC AGTATCTGAC TGACATCCTC GGGGACAAGC TGGACCTCTC CTCAGTGAGC 
AGCGAAGATG CCACCATGCT TCCATCTCCA CAGATGCTCA AGGGCAAGAT CCTGGTGAAG 
GGGAAGAAGC TCCCTGCCAA TATCAGTGAG GATGCAGAAG AAGGCGAAGT ATCTGATGAG 
GACAGTGCTG ATGAGATGGA GGATGACTGC AAGCTCCTCA ATGGGGATGC CTCCACCAAT 
CGGAAGCGTG TGGAAAACAT TGCAAAGAAG AAGCTGGATT CTCTGATCAA GGAGTCAAAG 
ATCCGTGACT GTGAAGACCC CAATGACTTC TCTGTGTCCA CACTATCCCC CTCTGGAAAG 
CTTGGGCGCA AAGCAGAGGC CAAGAAGGGT CAGAGCAAGG TTGAGGAAGA CGTGGAGGCC 
GGGGAAGACA GCGGGGTGAG CAGGCAGAAT AGCCGCCTCT TCATGAGCAG CTTCTCCAAG 
CGCAAGAAAA AAGGCAGCAA GATAAAGAAG GTGGCCAGCG TGGAAGAGGG GGACGAGACC 
CTGGACTCCC CAGGAAGCCA GAGCCGAGGG ACTGCCCGGC AAAAGAAGAC CATGAAGCTG 
TCACGAGCCC TCTCGGACCT AGTGAAATAT ACCAAGTCTG TGGGGACCCA TGACGTGGAG 
ATAGAGGTGG TATCTAGCTG GCAGGTGTCG TCCTTCAGTG AGACCAAGGC CCATCAGATC 
CTGCAGCAGA AGCCCACCCA GTACCTGCGC TTCAACCAGC ACCAGCTCTC GCGCATATAC 
CCCTCCTCCT ACCGTGTGGA CTCCAGCAAC TACAATCCAC AACCCTTCTG GAACGCTGGT 
TGCCAGATGG TTGCCCTGAA CTACCAGTCA GAGGGGCGGA TGCTACAGCT GAACAGGGCC 
AAGTTCAGCG CCAACGGTGA CTGTGGCTAC GTGCTCAAAC CCCAGTGCAT GTGCCAGGGT 
GTCTTCAACC CCAACTCGGA GGATCCCCTG CCGGGGCAGC TCAAGAAGCA GCTGGCCCTG 
AGGATCATCA GTGGCCAGCA GCTGCCCAAA CCACGGGACT CGGTGCTGGG CGACCGTGGG 
GAGATCATCG ACCCCTTCGT GGAGGTGGAG GTCATTGGGC TCCCCGTGGA CTGCAGCAAG 
GAGCAGACCC GAGTGGTGGA CGACAACGGA TTCAACCCCA TGTGGGAGGA GACACTGGTG 
TTCACCGTGC ACATGCCAGA GATTGCGCTT GTACGCTTCC TGGTCTGGGA CCATGACCCC 
ATTGGACGTG ACTTCATCGG CCAGAGGACA CTAGCCTTCA GCAGCATAAT GCCAGGCTAC 
CGGCATGTGT ACCTAGAAGG GATGGAAGAG GCCTCTATCT TTGTTCATGT GGCTGTCAGT 
GACATTAGTG GTAAGGTCAA GCAGACTCTG GGTCTAAAAG GTCTCTTCCT CCGAGGCACA 
AAGCCAGGCT CGCTGGACAG TCATGCTGCT GGACAGCCCC TCCCCCGGCC CTCCGTTAGC 
CAGAGGCTCC TGCGGCGCAC GGCCAGTGCC CCGACCAAAA GCCAGAAGCC AAGTCGCAAG 
GGCTTCCCAG AGCTGGCCCT GGGCACACAG GACGCAGGCT CCGAGGGGGC AGCTGATGAC 
GTGGCACCCT CTAGCCCCAA CCCAGCTCTG GAGGCCCCTA CTCAAGAGAG GTCAGGCAGC 
AGCAGCCCCC GAGGTAAGGC ACCAGGAGGA GAAGCAACAG AAGAGAGGAC ACTGGCACAG 
GTGCGGTCCC CAAATGCCCC CGAAGGCCCT GGACCTGCAG GGATGGCCGC CACATGCATG 
AAGTGTGTGG TGGGCTCCTG CGCCGGTATG GACGTTGAGG GCCTTCAAAG GGAGCAACAA 
CCCAGCCCTG GGCCTGCAGG CAGCCACATG GCCATCAGCC ATCAGCCCAG GGCCCGGGTA 
GATTCACTGG GGGGCCCTTG CTGCAGCCCA AGTCCTCGAG CCACCCCAGG GAGAAGCAAA 
GAGGCCCCCA AGGGTCCTAG GGCTCGGCGC CAGGGTCCAG GCGGCGGCTC TGTGTCCTCG 
GACTCCAGCA GCCCAGACAG CCCAGGCAGC CCCAAGGTGG CCCCCTGCCA GCCGGAGGGT 
GCTCACAGGC AGCAGGGGGC GCTGCAGGGA GAGATGAACG CCTTGTTCGT TCAAAAGCTG 
GAGGAGATCA GGAGTCATTC CCCCATGTTC TCCACCGATA CTCGCCTCTT CCCCCTGCAG 
CGGCCCATCT CCCCCCTGTG CAGCCTGGAA CCCATTGCCG AGGAACCAGC CCTGGGCCCT 
GGCCTCCCGT TACAGGCAGC TGCCCCCACA GGTCCATCTC AGGAAGGGTC CCAGTGCCCT 
GTAGGCCTGG GAGCCAAAGT GACCAGCTCC CAGCAGACAT CTCTGGGAGC TTTTGGAACC 
CTCCAGCTCA GGATTGGGGG CGGTAGGGAA AACGAAGAGC CACCCCTCAG GCCACACAAT 
GGTGGGATCT CCAGCGGGCC ACGTGAGGGG ACTTCTGGAA GACAGACAGA CAGCAAGAGC 
CGCTCCCGGG TCCCGGGCCA CTTGCCAGTG GTTAGGAGGG CCAAGAGTGA GGGCCAGGTG 
CTCTCGGAGC TCTCTCCCAC ACCTGCAGTG TACTCGGATG CCACGGGCAC TGACAGGTTA 
TGGCAGCGGC TGGAGCCTGG GAGCCACAGG GACAGTGTCT CCTCATCCTC CAGCATGTCC 
TCCAACGACA CCGTCATAGA CCTATCACTG CCCAGCCTCG GCCTCTGCCG CAGCCGAGAG 
AGCATTCCAG GGGTCTCTCT TGGACGCCTA ACCTCACGGC CCTGCTTAGC CTCAGCTGCC 
CGTCCTGACC TGCCTCCTGT GACCAAGAGC AAATCGAACC CCAACCTGCG GGTTGCGGGT 
GGGCTGCCTA CTGCACCCGA CGAGCTGCAG CCCCGGCCTC TTGCCCCGCG GCTGACCGGC 
CACCATCCAC GGCCACCGTG GCATCACCTC ACCCTGGTGG GCCTGCGGGA CTGCCCAGTG 
TCTGCTAAGT CCAAGAGTCT GGGAGACCTG ACAGCTGACG ACTTTGCTCC CAGCTTTCAG 
GGCAGCACCA GCAGCCTGAG CTGCGGCCTA GGCTCCCTGG GTGTGGCGCA TCAGGTGCTG 
GAGCCGGGAA TACGGCGAGA TGCTCTCACG GAGCAGCTAC GCTGGCTCAC CGGCTTCCAG 
CAGGCTGGGG ACATCACGTC GCCCACCAGC TTGGGCCCAG CTGGGGACGG GTCTGTAGGG 
GGCCCCAGCT TCCTGAGGCG TTCCTCCTCT CGGAGCCAGA GCCGCGTGCG CGCTATTGCC 
AGCCGAGCCC GCCAAGCGCA GGAGCGCCAG CAGAGGCTGA GAGGCCAGGA CTCACGGGGT 
CCCCCAGAGG AGGAGCGGGG CACCCCTGAG GGTGCCTGTT CTGTAGGCCA TGAGGGCTGT 
GTGGATGTGC CAATGCCTGC CAAGGGAGCC CCTGAGCAGG TGTGTGGTGC TGCTGATGGC 
CAGCTGCTGC TCAGGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250270.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248572.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X4, mRNA.

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CloneID	OMu74425
Clone ID Related Accession (Same CDS sequence)	XM_011250271.2 , XM_011250271.3 , XM_011250270.1
Accession Version	XM_011250270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGCT CCATTGGTCC CGCTTCATTG CCTGAGGACC CAGGGCCGGA TCTAGGCAGC 
AGATGGCTTT TCCTGAGTGC CAACGTTTTG CCTGTGGTGG AGCGATGCAT GAGTGCCATG 
CAAGAGGGGA CCCAGATGGT GAAGCTCCGT GGCAGCTCCA AGGGATTGGT CCGCTTCTAC 
TACCTGGATG AGCACCGCTC CTGCCTCCGA TGGCGTCCCT CCCGCAAGAA CGAGAAAGCC 
AAGATTTCCA TCGACTCCAT CCAGGAGGTG AGTGAGGGCC GGCAGTCTGA AATCTTCCAG 
CGCTACCCGG ACAGCAGCTT TGACCCCAAC TGCTGCTTCA GCATCTACCA TGGCAGCCAC 
AGAGAGTCGC TGGACTTGGT CTCACCCAGC AGTGAAGAGG CACGTACCTG GGTCACCGGC 
CTTCGCTACC TCATGGCTGG CATCAGCGAT GAAGACAGCC TAGCCCGTCG CCAGCGTACC 
AGGGACCAGT GGCTGAAGCA GACGTTTGAT GAGGCTGACA AGAACGGGGA CGGCAGCCTG 
AGCATCAGTG AGGTTCTGCA GCTGCTACAT AAACTCAACG TGAACCTGCC CCGGCAGAGG 
GTGAAACAGA TGTTCAGGGA GGCGGACACA GATGACCATC AGGGGACATT AGGCTTTGAG 
GAATTCTGCG CCTTCTACAA GATGATGTCT ACCCGCCGAG ACCTCTACCT GCTCATGCTG 
ACCTACAGCA ACCATAAGGA CCACTTGGAT GCCTCCGACC TGCAGCGCTT TCTGGAGGTG 
GAGCAGAAGA TGAACGGTGT GACCCTGGAG AGCTGTCAGA ACATCATTGA GCAGTTTGAG 
CCTTGCCTGG AAAATAAGAG CAAGGGGATG CTGGGGATTG ATGGTAGTCG TGAGCCGAGC 
CCAGTGATGG AGATACACCA CGGCGCTCCT GTGTACACCG CAGGCTTTAC CAACTACACC 
CGGAGCCCCG CCGGTGACAT CTTCAACCCT GAGCACAACA GAGTGCACCA AGACATGACG 
CAGCCGCTGA GTCACTACTT CATCACCTCG TCCCACAACA CCTACCTCGT GGGTGACCAG 
CTCATGTCCC AGTCTCGGGT GGACATGTAT GCCTGGGTCC TGCAGGCCGG CTGCCGCTGT 
GTGGAGGTGG ACTGCTGGGA CGGGCCCGAT GGAGAACCCA TTGTCCATCA TGGCTACACT 
CTGACCTCCA AGATCCTTTT CAAAGATGTC ATCGAAACCA TCAACAAATA CGCCTTCATC 
AAGAATGAGT ACCCAGTGAT CCTGTCCATT GAGAACCACT GTAGTGTTGT TCAGCAGAAG 
AAGATGGCCC AGTATCTGAC TGACATCCTC GGGGACAAGC TGGACCTCTC CTCAGTGAGC 
AGCGAAGATG CCACCATGCT TCCATCTCCA CAGATGCTCA AGGGCAAGAT CCTGGTGAAG 
GGGAAGAAGC TCCCTGCCAA TATCAGTGAG GATGCAGAAG AAGGCGAAGT ATCTGATGAG 
GACAGTGCTG ATGAGATGGA GGATGACTGC AAGCTCCTCA ATGGGGATGC CTCCACCAAT 
CGGAAGCGTG TGGAAAACAT TGCAAAGAAG AAGCTGGATT CTCTGATCAA GGAGTCAAAG 
ATCCGTGACT GTGAAGACCC CAATGACTTC TCTGTGTCCA CACTATCCCC CTCTGGAAAG 
CTTGGGCGCA AAGCAGAGGC CAAGAAGGGT CAGAGCAAGG TTGAGGAAGA CGTGGAGGCC 
GGGGAAGACA GCGGGGTGAG CAGGCAGAAT AGCCGCCTCT TCATGAGCAG CTTCTCCAAG 
CGCAAGAAAA AAGGCAGCAA GATAAAGAAG GTGGCCAGCG TGGAAGAGGG GGACGAGACC 
CTGGACTCCC CAGGAAGCCA GAGCCGAGGG ACTGCCCGGC AAAAGAAGAC CATGAAGCTG 
TCACGAGCCC TCTCGGACCT AGTGAAATAT ACCAAGTCTG TGGGGACCCA TGACGTGGAG 
ATAGAGGTGG TATCTAGCTG GCAGGTGTCG TCCTTCAGTG AGACCAAGGC CCATCAGATC 
CTGCAGCAGA AGCCCACCCA GTACCTGCGC TTCAACCAGC ACCAGCTCTC GCGCATATAC 
CCCTCCTCCT ACCGTGTGGA CTCCAGCAAC TACAATCCAC AACCCTTCTG GAACGCTGGT 
TGCCAGATGG TTGCCCTGAA CTACCAGTCA GAGGGGCGGA TGCTACAGCT GAACAGGGCC 
AAGTTCAGCG CCAACGGTGA CTGTGGCTAC GTGCTCAAAC CCCAGTGCAT GTGCCAGGGT 
GTCTTCAACC CCAACTCGGA GGATCCCCTG CCGGGGCAGC TCAAGAAGCA GCTGGCCCTG 
AGGATCATCA GTGGCCAGCA GCTGCCCAAA CCACGGGACT CGGTGCTGGG CGACCGTGGG 
GAGATCATCG ACCCCTTCGT GGAGGTGGAG GTCATTGGGC TCCCCGTGGA CTGCAGCAAG 
GAGCAGACCC GAGTGGTGGA CGACAACGGA TTCAACCCCA TGTGGGAGGA GACACTGGTG 
TTCACCGTGC ACATGCCAGA GATTGCGCTT GTACGCTTCC TGGTCTGGGA CCATGACCCC 
ATTGGACGTG ACTTCATCGG CCAGAGGACA CTAGCCTTCA GCAGCATAAT GCCAGGCTAC 
CGGCATGTGT ACCTAGAAGG GATGGAAGAG GCCTCTATCT TTGTTCATGT GGCTGTCAGT 
GACATTAGTG GTAAGGTCAA GCAGACTCTG GGTCTAAAAG GTCTCTTCCT CCGAGGCACA 
AAGCCAGGCT CGCTGGACAG TCATGCTGCT GGACAGCCCC TCCCCCGGCC CTCCGTTAGC 
CAGAGGCTCC TGCGGCGCAC GGCCAGTGCC CCGACCAAAA GCCAGAAGCC AAGTCGCAAG 
GGCTTCCCAG AGCTGGCCCT GGGCACACAG GACGCAGGCT CCGAGGGGGC AGCTGATGAC 
GTGGCACCCT CTAGCCCCAA CCCAGCTCTG GAGGCCCCTA CTCAAGAGAG GTCAGGCAGC 
AGCAGCCCCC GAGGTAAGGC ACCAGGAGGA GAAGCAACAG AAGAGAGGAC ACTGGCACAG 
GTGCGGTCCC CAAATGCCCC CGAAGGCCCT GGACCTGCAG GGATGGCCGC CACATGCATG 
AAGTGTGTGG TGGGCTCCTG CGCCGGTATG GACGTTGAGG GCCTTCAAAG GGAGCAACAA 
CCCAGCCCTG GGCCTGCAGG CAGCCACATG GCCATCAGCC ATCAGCCCAG GGCCCGGGTA 
GATTCACTGG GGGGCCCTTG CTGCAGCCCA AGTCCTCGAG CCACCCCAGG GAGAAGCAAA 
GAGGCCCCCA AGGGTCCTAG GGCTCGGCGC CAGGGTCCAG GCGGCGGCTC TGTGTCCTCG 
GACTCCAGCA GCCCAGACAG CCCAGGCAGC CCCAAGGTGG CCCCCTGCCA GCCGGAGGGT 
GCTCACAGGC AGCAGGGGGC GCTGCAGGGA GAGATGAACG CCTTGTTCGT TCAAAAGCTG 
GAGGAGATCA GGAGTCATTC CCCCATGTTC TCCACCGATA CTCGCCTCTT CCCCCTGCAG 
CGGCCCATCT CCCCCCTGTG CAGCCTGGAA CCCATTGCCG AGGAACCAGC CCTGGGCCCT 
GGCCTCCCGT TACAGGCAGC TGCCCCCACA GGTCCATCTC AGGAAGGGTC CCAGTGCCCT 
GTAGGCCTGG GAGCCAAAGT GACCAGCTCC CAGCAGACAT CTCTGGGAGC TTTTGGAACC 
CTCCAGCTCA GGATTGGGGG CGGTAGGGAA AACGAAGAGC CACCCCTCAG GCCACACAAT 
GGTGGGATCT CCAGCGGGCC ACGTGAGGGG ACTTCTGGAA GACAGACAGA CAGCAAGAGC 
CGCTCCCGGG TCCCGGGCCA CTTGCCAGTG GTTAGGAGGG CCAAGAGTGA GGGCCAGGTG 
CTCTCGGAGC TCTCTCCCAC ACCTGCAGTG TACTCGGATG CCACGGGCAC TGACAGGTTA 
TGGCAGCGGC TGGAGCCTGG GAGCCACAGG GACAGTGTCT CCTCATCCTC CAGCATGTCC 
TCCAACGACA CCGTCATAGA CCTATCACTG CCCAGCCTCG GCCTCTGCCG CAGCCGAGAG 
AGCATTCCAG GGGTCTCTCT TGGACGCCTA ACCTCACGGC CCTGCTTAGC CTCAGCTGCC 
CGTCCTGACC TGCCTCCTGT GACCAAGAGC AAATCGAACC CCAACCTGCG GGTTGCGGGT 
GGGCTGCCTA CTGCACCCGA CGAGCTGCAG CCCCGGCCTC TTGCCCCGCG GCTGACCGGC 
CACCATCCAC GGCCACCGTG GCATCACCTC ACCCTGGTGG GCCTGCGGGA CTGCCCAGTG 
TCTGCTAAGT CCAAGAGTCT GGGAGACCTG ACAGCTGACG ACTTTGCTCC CAGCTTTCAG 
GGCAGCACCA GCAGCCTGAG CTGCGGCCTA GGCTCCCTGG GTGTGGCGCA TCAGGTGCTG 
GAGCCGGGAA TACGGCGAGA TGCTCTCACG GAGCAGCTAC GCTGGCTCAC CGGCTTCCAG 
CAGGCTGGGG ACATCACGTC GCCCACCAGC TTGGGCCCAG CTGGGGACGG GTCTGTAGGG 
GGCCCCAGCT TCCTGAGGCG TTCCTCCTCT CGGAGCCAGA GCCGCGTGCG CGCTATTGCC 
AGCCGAGCCC GCCAAGCGCA GGAGCGCCAG CAGAGGCTGA GAGGCCAGGA CTCACGGGGT 
CCCCCAGAGG AGGAGCGGGG CACCCCTGAG GGTGCCTGTT CTGTAGGCCA TGAGGGCTGT 
GTGGATGTGC CAATGCCTGC CAAGGGAGCC CCTGAGCAGG TGTGTGGTGC TGCTGATGGC 
CAGCTGCTGC TCAGGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248572.1
CDS	275..5095
Translation	MDSSIGPASLPEDPGPDLGSRWLFLSANVLPVVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	XM_011250270.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250270.1

ORF Insert Sequence:

ATGGATAGCT CCATTGGTCC CGCTTCATTG CCTGAGGACC CAGGGCCGGA TCTAGGCAGC 
AGATGGCTTT TCCTGAGTGC CAACGTTTTG CCTGTGGTGG AGCGATGCAT GAGTGCCATG 
CAAGAGGGGA CCCAGATGGT GAAGCTCCGT GGCAGCTCCA AGGGATTGGT CCGCTTCTAC 
TACCTGGATG AGCACCGCTC CTGCCTCCGA TGGCGTCCCT CCCGCAAGAA CGAGAAAGCC 
AAGATTTCCA TCGACTCCAT CCAGGAGGTG AGTGAGGGCC GGCAGTCTGA AATCTTCCAG 
CGCTACCCGG ACAGCAGCTT TGACCCCAAC TGCTGCTTCA GCATCTACCA TGGCAGCCAC 
AGAGAGTCGC TGGACTTGGT CTCACCCAGC AGTGAAGAGG CACGTACCTG GGTCACCGGC 
CTTCGCTACC TCATGGCTGG CATCAGCGAT GAAGACAGCC TAGCCCGTCG CCAGCGTACC 
AGGGACCAGT GGCTGAAGCA GACGTTTGAT GAGGCTGACA AGAACGGGGA CGGCAGCCTG 
AGCATCAGTG AGGTTCTGCA GCTGCTACAT AAACTCAACG TGAACCTGCC CCGGCAGAGG 
GTGAAACAGA TGTTCAGGGA GGCGGACACA GATGACCATC AGGGGACATT AGGCTTTGAG 
GAATTCTGCG CCTTCTACAA GATGATGTCT ACCCGCCGAG ACCTCTACCT GCTCATGCTG 
ACCTACAGCA ACCATAAGGA CCACTTGGAT GCCTCCGACC TGCAGCGCTT TCTGGAGGTG 
GAGCAGAAGA TGAACGGTGT GACCCTGGAG AGCTGTCAGA ACATCATTGA GCAGTTTGAG 
CCTTGCCTGG AAAATAAGAG CAAGGGGATG CTGGGGATTG ATGGTAGTCG TGAGCCGAGC 
CCAGTGATGG AGATACACCA CGGCGCTCCT GTGTACACCG CAGGCTTTAC CAACTACACC 
CGGAGCCCCG CCGGTGACAT CTTCAACCCT GAGCACAACA GAGTGCACCA AGACATGACG 
CAGCCGCTGA GTCACTACTT CATCACCTCG TCCCACAACA CCTACCTCGT GGGTGACCAG 
CTCATGTCCC AGTCTCGGGT GGACATGTAT GCCTGGGTCC TGCAGGCCGG CTGCCGCTGT 
GTGGAGGTGG ACTGCTGGGA CGGGCCCGAT GGAGAACCCA TTGTCCATCA TGGCTACACT 
CTGACCTCCA AGATCCTTTT CAAAGATGTC ATCGAAACCA TCAACAAATA CGCCTTCATC 
AAGAATGAGT ACCCAGTGAT CCTGTCCATT GAGAACCACT GTAGTGTTGT TCAGCAGAAG 
AAGATGGCCC AGTATCTGAC TGACATCCTC GGGGACAAGC TGGACCTCTC CTCAGTGAGC 
AGCGAAGATG CCACCATGCT TCCATCTCCA CAGATGCTCA AGGGCAAGAT CCTGGTGAAG 
GGGAAGAAGC TCCCTGCCAA TATCAGTGAG GATGCAGAAG AAGGCGAAGT ATCTGATGAG 
GACAGTGCTG ATGAGATGGA GGATGACTGC AAGCTCCTCA ATGGGGATGC CTCCACCAAT 
CGGAAGCGTG TGGAAAACAT TGCAAAGAAG AAGCTGGATT CTCTGATCAA GGAGTCAAAG 
ATCCGTGACT GTGAAGACCC CAATGACTTC TCTGTGTCCA CACTATCCCC CTCTGGAAAG 
CTTGGGCGCA AAGCAGAGGC CAAGAAGGGT CAGAGCAAGG TTGAGGAAGA CGTGGAGGCC 
GGGGAAGACA GCGGGGTGAG CAGGCAGAAT AGCCGCCTCT TCATGAGCAG CTTCTCCAAG 
CGCAAGAAAA AAGGCAGCAA GATAAAGAAG GTGGCCAGCG TGGAAGAGGG GGACGAGACC 
CTGGACTCCC CAGGAAGCCA GAGCCGAGGG ACTGCCCGGC AAAAGAAGAC CATGAAGCTG 
TCACGAGCCC TCTCGGACCT AGTGAAATAT ACCAAGTCTG TGGGGACCCA TGACGTGGAG 
ATAGAGGTGG TATCTAGCTG GCAGGTGTCG TCCTTCAGTG AGACCAAGGC CCATCAGATC 
CTGCAGCAGA AGCCCACCCA GTACCTGCGC TTCAACCAGC ACCAGCTCTC GCGCATATAC 
CCCTCCTCCT ACCGTGTGGA CTCCAGCAAC TACAATCCAC AACCCTTCTG GAACGCTGGT 
TGCCAGATGG TTGCCCTGAA CTACCAGTCA GAGGGGCGGA TGCTACAGCT GAACAGGGCC 
AAGTTCAGCG CCAACGGTGA CTGTGGCTAC GTGCTCAAAC CCCAGTGCAT GTGCCAGGGT 
GTCTTCAACC CCAACTCGGA GGATCCCCTG CCGGGGCAGC TCAAGAAGCA GCTGGCCCTG 
AGGATCATCA GTGGCCAGCA GCTGCCCAAA CCACGGGACT CGGTGCTGGG CGACCGTGGG 
GAGATCATCG ACCCCTTCGT GGAGGTGGAG GTCATTGGGC TCCCCGTGGA CTGCAGCAAG 
GAGCAGACCC GAGTGGTGGA CGACAACGGA TTCAACCCCA TGTGGGAGGA GACACTGGTG 
TTCACCGTGC ACATGCCAGA GATTGCGCTT GTACGCTTCC TGGTCTGGGA CCATGACCCC 
ATTGGACGTG ACTTCATCGG CCAGAGGACA CTAGCCTTCA GCAGCATAAT GCCAGGCTAC 
CGGCATGTGT ACCTAGAAGG GATGGAAGAG GCCTCTATCT TTGTTCATGT GGCTGTCAGT 
GACATTAGTG GTAAGGTCAA GCAGACTCTG GGTCTAAAAG GTCTCTTCCT CCGAGGCACA 
AAGCCAGGCT CGCTGGACAG TCATGCTGCT GGACAGCCCC TCCCCCGGCC CTCCGTTAGC 
CAGAGGCTCC TGCGGCGCAC GGCCAGTGCC CCGACCAAAA GCCAGAAGCC AAGTCGCAAG 
GGCTTCCCAG AGCTGGCCCT GGGCACACAG GACGCAGGCT CCGAGGGGGC AGCTGATGAC 
GTGGCACCCT CTAGCCCCAA CCCAGCTCTG GAGGCCCCTA CTCAAGAGAG GTCAGGCAGC 
AGCAGCCCCC GAGGTAAGGC ACCAGGAGGA GAAGCAACAG AAGAGAGGAC ACTGGCACAG 
GTGCGGTCCC CAAATGCCCC CGAAGGCCCT GGACCTGCAG GGATGGCCGC CACATGCATG 
AAGTGTGTGG TGGGCTCCTG CGCCGGTATG GACGTTGAGG GCCTTCAAAG GGAGCAACAA 
CCCAGCCCTG GGCCTGCAGG CAGCCACATG GCCATCAGCC ATCAGCCCAG GGCCCGGGTA 
GATTCACTGG GGGGCCCTTG CTGCAGCCCA AGTCCTCGAG CCACCCCAGG GAGAAGCAAA 
GAGGCCCCCA AGGGTCCTAG GGCTCGGCGC CAGGGTCCAG GCGGCGGCTC TGTGTCCTCG 
GACTCCAGCA GCCCAGACAG CCCAGGCAGC CCCAAGGTGG CCCCCTGCCA GCCGGAGGGT 
GCTCACAGGC AGCAGGGGGC GCTGCAGGGA GAGATGAACG CCTTGTTCGT TCAAAAGCTG 
GAGGAGATCA GGAGTCATTC CCCCATGTTC TCCACCGATA CTCGCCTCTT CCCCCTGCAG 
CGGCCCATCT CCCCCCTGTG CAGCCTGGAA CCCATTGCCG AGGAACCAGC CCTGGGCCCT 
GGCCTCCCGT TACAGGCAGC TGCCCCCACA GGTCCATCTC AGGAAGGGTC CCAGTGCCCT 
GTAGGCCTGG GAGCCAAAGT GACCAGCTCC CAGCAGACAT CTCTGGGAGC TTTTGGAACC 
CTCCAGCTCA GGATTGGGGG CGGTAGGGAA AACGAAGAGC CACCCCTCAG GCCACACAAT 
GGTGGGATCT CCAGCGGGCC ACGTGAGGGG ACTTCTGGAA GACAGACAGA CAGCAAGAGC 
CGCTCCCGGG TCCCGGGCCA CTTGCCAGTG GTTAGGAGGG CCAAGAGTGA GGGCCAGGTG 
CTCTCGGAGC TCTCTCCCAC ACCTGCAGTG TACTCGGATG CCACGGGCAC TGACAGGTTA 
TGGCAGCGGC TGGAGCCTGG GAGCCACAGG GACAGTGTCT CCTCATCCTC CAGCATGTCC 
TCCAACGACA CCGTCATAGA CCTATCACTG CCCAGCCTCG GCCTCTGCCG CAGCCGAGAG 
AGCATTCCAG GGGTCTCTCT TGGACGCCTA ACCTCACGGC CCTGCTTAGC CTCAGCTGCC 
CGTCCTGACC TGCCTCCTGT GACCAAGAGC AAATCGAACC CCAACCTGCG GGTTGCGGGT 
GGGCTGCCTA CTGCACCCGA CGAGCTGCAG CCCCGGCCTC TTGCCCCGCG GCTGACCGGC 
CACCATCCAC GGCCACCGTG GCATCACCTC ACCCTGGTGG GCCTGCGGGA CTGCCCAGTG 
TCTGCTAAGT CCAAGAGTCT GGGAGACCTG ACAGCTGACG ACTTTGCTCC CAGCTTTCAG 
GGCAGCACCA GCAGCCTGAG CTGCGGCCTA GGCTCCCTGG GTGTGGCGCA TCAGGTGCTG 
GAGCCGGGAA TACGGCGAGA TGCTCTCACG GAGCAGCTAC GCTGGCTCAC CGGCTTCCAG 
CAGGCTGGGG ACATCACGTC GCCCACCAGC TTGGGCCCAG CTGGGGACGG GTCTGTAGGG 
GGCCCCAGCT TCCTGAGGCG TTCCTCCTCT CGGAGCCAGA GCCGCGTGCG CGCTATTGCC 
AGCCGAGCCC GCCAAGCGCA GGAGCGCCAG CAGAGGCTGA GAGGCCAGGA CTCACGGGGT 
CCCCCAGAGG AGGAGCGGGG CACCCCTGAG GGTGCCTGTT CTGTAGGCCA TGAGGGCTGT 
GTGGATGTGC CAATGCCTGC CAAGGGAGCC CCTGAGCAGG TGTGTGGTGC TGCTGATGGC 
CAGCTGCTGC TCAGGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250273.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248575.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu74427
Clone ID Related Accession (Same CDS sequence)	XM_011250273.2
Accession Version	XM_011250273.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC CTGGGCCCCC TGGTGGGCTT AGCCAGGACC AAGTGGAGCG ATGCATGAGT 
GCCATGCAAG AGGGGACCCA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG ATTGGTCCGC 
TTCTACTACC TGGATGAGCA CCGCTCCTGC CTCCGATGGC GTCCCTCCCG CAAGAACGAG 
AAAGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGCCGGCA GTCTGAAATC 
TTCCAGCGCT ACCCGGACAG CAGCTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACAGAG AGTCGCTGGA CTTGGTCTCA CCCAGCAGTG AAGAGGCACG TACCTGGGTC 
ACCGGCCTTC GCTACCTCAT GGCTGGCATC AGCGATGAAG ACAGCCTAGC CCGTCGCCAG 
CGTACCAGGG ACCAGTGGCT GAAGCAGACG TTTGATGAGG CTGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCAGTGAGGT TCTGCAGCTG CTACATAAAC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AACAGATGTT CAGGGAGGCG GACACAGATG ACCATCAGGG GACATTAGGC 
TTTGAGGAAT TCTGCGCCTT CTACAAGATG ATGTCTACCC GCCGAGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA TAAGGACCAC TTGGATGCCT CCGACCTGCA GCGCTTTCTG 
GAGGTGGAGC AGAAGATGAA CGGTGTGACC CTGGAGAGCT GTCAGAACAT CATTGAGCAG 
TTTGAGCCTT GCCTGGAAAA TAAGAGCAAG GGGATGCTGG GGATTGATGG TAGTCGTGAG 
CCGAGCCCAG TGATGGAGAT ACACCACGGC GCTCCTGTGT ACACCGCAGG CTTTACCAAC 
TACACCCGGA GCCCCGCCGG TGACATCTTC AACCCTGAGC ACAACAGAGT GCACCAAGAC 
ATGACGCAGC CGCTGAGTCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTGGAC ATGTATGCCT GGGTCCTGCA GGCCGGCTGC 
CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCCGATGGAG AACCCATTGT CCATCATGGC 
TACACTCTGA CCTCCAAGAT CCTTTTCAAA GATGTCATCG AAACCATCAA CAAATACGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGTAG TGTTGTTCAG 
CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATCCTCGGGG ACAAGCTGGA CCTCTCCTCA 
GTGAGCAGCG AAGATGCCAC CATGCTTCCA TCTCCACAGA TGCTCAAGGG CAAGATCCTG 
GTGAAGGGGA AGAAGCTCCC TGCCAATATC AGTGAGGATG CAGAAGAAGG CGAAGTATCT 
GATGAGGACA GTGCTGATGA GATGGAGGAT GACTGCAAGC TCCTCAATGG GGATGCCTCC 
ACCAATCGGA AGCGTGTGGA AAACATTGCA AAGAAGAAGC TGGATTCTCT GATCAAGGAG 
TCAAAGATCC GTGACTGTGA AGACCCCAAT GACTTCTCTG TGTCCACACT ATCCCCCTCT 
GGAAAGCTTG GGCGCAAAGC AGAGGCCAAG AAGGGTCAGA GCAAGGTTGA GGAAGACGTG 
GAGGCCGGGG AAGACAGCGG GGTGAGCAGG CAGAATAGCC GCCTCTTCAT GAGCAGCTTC 
TCCAAGCGCA AGAAAAAAGG CAGCAAGATA AAGAAGGTGG CCAGCGTGGA AGAGGGGGAC 
GAGACCCTGG ACTCCCCAGG AAGCCAGAGC CGAGGGACTG CCCGGCAAAA GAAGACCATG 
AAGCTGTCAC GAGCCCTCTC GGACCTAGTG AAATATACCA AGTCTGTGGG GACCCATGAC 
GTGGAGATAG AGGTGGTATC TAGCTGGCAG GTGTCGTCCT TCAGTGAGAC CAAGGCCCAT 
CAGATCCTGC AGCAGAAGCC CACCCAGTAC CTGCGCTTCA ACCAGCACCA GCTCTCGCGC 
ATATACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ATCCACAACC CTTCTGGAAC 
GCTGGTTGCC AGATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT ACAGCTGAAC 
AGGGCCAAGT TCAGCGCCAA CGGTGACTGT GGCTACGTGC TCAAACCCCA GTGCATGTGC 
CAGGGTGTCT TCAACCCCAA CTCGGAGGAT CCCCTGCCGG GGCAGCTCAA GAAGCAGCTG 
GCCCTGAGGA TCATCAGTGG CCAGCAGCTG CCCAAACCAC GGGACTCGGT GCTGGGCGAC 
CGTGGGGAGA TCATCGACCC CTTCGTGGAG GTGGAGGTCA TTGGGCTCCC CGTGGACTGC 
AGCAAGGAGC AGACCCGAGT GGTGGACGAC AACGGATTCA ACCCCATGTG GGAGGAGACA 
CTGGTGTTCA CCGTGCACAT GCCAGAGATT GCGCTTGTAC GCTTCCTGGT CTGGGACCAT 
GACCCCATTG GACGTGACTT CATCGGCCAG AGGACACTAG CCTTCAGCAG CATAATGCCA 
GGCTACCGGC ATGTGTACCT AGAAGGGATG GAAGAGGCCT CTATCTTTGT TCATGTGGCT 
GTCAGTGACA TTAGTGGTAA GGTCAAGCAG ACTCTGGGTC TAAAAGGTCT CTTCCTCCGA 
GGCACAAAGC CAGGCTCGCT GGACAGTCAT GCTGCTGGAC AGCCCCTCCC CCGGCCCTCC 
GTTAGCCAGA GGCTCCTGCG GCGCACGGCC AGTGCCCCGA CCAAAAGCCA GAAGCCAAGT 
CGCAAGGGCT TCCCAGAGCT GGCCCTGGGC ACACAGGACG CAGGCTCCGA GGGGGCAGCT 
GATGACGTGG CACCCTCTAG CCCCAACCCA GCTCTGGAGG CCCCTACTCA AGAGAGGTCA 
GGCAGCAGCA GCCCCCGAGG TAAGGCACCA GGAGGAGAAG CAACAGAAGA GAGGACACTG 
GCACAGGTGC GGTCCCCAAA TGCCCCCGAA GGCCCTGGAC CTGCAGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG CTCCTGCGCC GGTATGGACG TTGAGGGCCT TCAAAGGGAG 
CAACAACCCA GCCCTGGGCC TGCAGGCAGC CACATGGCCA TCAGCCATCA GCCCAGGGCC 
CGGGTAGATT CACTGGGGGG CCCTTGCTGC AGCCCAAGTC CTCGAGCCAC CCCAGGGAGA 
AGCAAAGAGG CCCCCAAGGG TCCTAGGGCT CGGCGCCAGG GTCCAGGCGG CGGCTCTGTG 
TCCTCGGACT CCAGCAGCCC AGACAGCCCA GGCAGCCCCA AGGTGGCCCC CTGCCAGCCG 
GAGGGTGCTC ACAGGCAGCA GGGGGCGCTG CAGGGAGAGA TGAACGCCTT GTTCGTTCAA 
AAGCTGGAGG AGATCAGGAG TCATTCCCCC ATGTTCTCCA CCGATACTCG CCTCTTCCCC 
CTGCAGCGGC CCATCTCCCC CCTGTGCAGC CTGGAACCCA TTGCCGAGGA ACCAGCCCTG 
GGCCCTGGCC TCCCGTTACA GGCAGCTGCC CCCACAGGTC CATCTCAGGA AGGGTCCCAG 
TGCCCTGTAG GCCTGGGAGC CAAAGTGACC AGCTCCCAGC AGACATCTCT GGGAGCTTTT 
GGAACCCTCC AGCTCAGGAT TGGGGGCGGT AGGGAAAACG AAGAGCCACC CCTCAGGCCA 
CACAATGGTG GGATCTCCAG CGGGCCACGT GAGGGGACTT CTGGAAGACA GACAGACAGC 
AAGAGCCGCT CCCGGGTCCC GGGCCACTTG CCAGTGGTTA GGAGGGCCAA GAGTGAGGGC 
CAGGTGCTCT CGGAGCTCTC TCCCACACCT GCAGTGTACT CGGATGCCAC GGGCACTGAC 
AGGTTATGGC AGCGGCTGGA GCCTGGGAGC CACAGGGACA GTGTCTCCTC ATCCTCCAGC 
ATGTCCTCCA ACGACACCGT CATAGACCTA TCACTGCCCA GCCTCGGCCT CTGCCGCAGC 
CGAGAGAGCA TTCCAGGGGT CTCTCTTGGA CGCCTAACCT CACGGCCCTG CTTAGCCTCA 
GCTGCCCGTC CTGACCTGCC TCCTGTGACC AAGAGCAAAT CGAACCCCAA CCTGCGGGTT 
GCGGGTGGGC TGCCTACTGC ACCCGACGAG CTGCAGCCCC GGCCTCTTGC CCCGCGGCTG 
ACCGGCCACC ATCCACGGCC ACCGTGGCAT CACCTCACCC TGGTGGGCCT GCGGGACTGC 
CCAGTGTCTG CTAAGTCCAA GAGTCTGGGA GACCTGACAG CTGACGACTT TGCTCCCAGC 
TTTCAGGGCA GCACCAGCAG CCTGAGCTGC GGCCTAGGCT CCCTGGGTGT GGCGCATCAG 
GTGCTGGAGC CGGGAATACG GCGAGATGCT CTCACGGAGC AGCTACGCTG GCTCACCGGC 
TTCCAGCAGG CTGGGGACAT CACGTCGCCC ACCAGCTTGG GCCCAGCTGG GGACGGGTCT 
GTAGGGGGCC CCAGCTTCCT GAGGCGTTCC TCCTCTCGGA GCCAGAGCCG CGTGCGCGCT 
ATTGCCAGCC GAGCCCGCCA AGCGCAGGAG CGCCAGCAGA GGCTGAGAGG CCAGGACTCA 
CGGGGTCCCC CAGAGGAGGA GCGGGGCACC CCTGAGGGTG CCTGTTCTGT AGGCCATGAG 
GGCTGTGTGG ATGTGCCAAT GCCTGCCAAG GGAGCCCCTG AGCAGGTGTG TGGTGCTGCT 
GATGGCCAGC TGCTGCTCAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248575.1
CDS	194..4960
Translation	MEEPGPPGGLSQDQVERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	XM_011250273.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250273.2

ORF Insert Sequence:

ATGGAAGAGC CTGGGCCCCC TGGTGGGCTT AGCCAGGACC AAGTGGAGCG ATGCATGAGT 
GCCATGCAAG AGGGGACCCA GATGGTGAAG CTCCGTGGCA GCTCCAAGGG ATTGGTCCGC 
TTCTACTACC TGGATGAGCA CCGCTCCTGC CTCCGATGGC GTCCCTCCCG CAAGAACGAG 
AAAGCCAAGA TTTCCATCGA CTCCATCCAG GAGGTGAGTG AGGGCCGGCA GTCTGAAATC 
TTCCAGCGCT ACCCGGACAG CAGCTTTGAC CCCAACTGCT GCTTCAGCAT CTACCATGGC 
AGCCACAGAG AGTCGCTGGA CTTGGTCTCA CCCAGCAGTG AAGAGGCACG TACCTGGGTC 
ACCGGCCTTC GCTACCTCAT GGCTGGCATC AGCGATGAAG ACAGCCTAGC CCGTCGCCAG 
CGTACCAGGG ACCAGTGGCT GAAGCAGACG TTTGATGAGG CTGACAAGAA CGGGGACGGC 
AGCCTGAGCA TCAGTGAGGT TCTGCAGCTG CTACATAAAC TCAACGTGAA CCTGCCCCGG 
CAGAGGGTGA AACAGATGTT CAGGGAGGCG GACACAGATG ACCATCAGGG GACATTAGGC 
TTTGAGGAAT TCTGCGCCTT CTACAAGATG ATGTCTACCC GCCGAGACCT CTACCTGCTC 
ATGCTGACCT ACAGCAACCA TAAGGACCAC TTGGATGCCT CCGACCTGCA GCGCTTTCTG 
GAGGTGGAGC AGAAGATGAA CGGTGTGACC CTGGAGAGCT GTCAGAACAT CATTGAGCAG 
TTTGAGCCTT GCCTGGAAAA TAAGAGCAAG GGGATGCTGG GGATTGATGG TAGTCGTGAG 
CCGAGCCCAG TGATGGAGAT ACACCACGGC GCTCCTGTGT ACACCGCAGG CTTTACCAAC 
TACACCCGGA GCCCCGCCGG TGACATCTTC AACCCTGAGC ACAACAGAGT GCACCAAGAC 
ATGACGCAGC CGCTGAGTCA CTACTTCATC ACCTCGTCCC ACAACACCTA CCTCGTGGGT 
GACCAGCTCA TGTCCCAGTC TCGGGTGGAC ATGTATGCCT GGGTCCTGCA GGCCGGCTGC 
CGCTGTGTGG AGGTGGACTG CTGGGACGGG CCCGATGGAG AACCCATTGT CCATCATGGC 
TACACTCTGA CCTCCAAGAT CCTTTTCAAA GATGTCATCG AAACCATCAA CAAATACGCC 
TTCATCAAGA ATGAGTACCC AGTGATCCTG TCCATTGAGA ACCACTGTAG TGTTGTTCAG 
CAGAAGAAGA TGGCCCAGTA TCTGACTGAC ATCCTCGGGG ACAAGCTGGA CCTCTCCTCA 
GTGAGCAGCG AAGATGCCAC CATGCTTCCA TCTCCACAGA TGCTCAAGGG CAAGATCCTG 
GTGAAGGGGA AGAAGCTCCC TGCCAATATC AGTGAGGATG CAGAAGAAGG CGAAGTATCT 
GATGAGGACA GTGCTGATGA GATGGAGGAT GACTGCAAGC TCCTCAATGG GGATGCCTCC 
ACCAATCGGA AGCGTGTGGA AAACATTGCA AAGAAGAAGC TGGATTCTCT GATCAAGGAG 
TCAAAGATCC GTGACTGTGA AGACCCCAAT GACTTCTCTG TGTCCACACT ATCCCCCTCT 
GGAAAGCTTG GGCGCAAAGC AGAGGCCAAG AAGGGTCAGA GCAAGGTTGA GGAAGACGTG 
GAGGCCGGGG AAGACAGCGG GGTGAGCAGG CAGAATAGCC GCCTCTTCAT GAGCAGCTTC 
TCCAAGCGCA AGAAAAAAGG CAGCAAGATA AAGAAGGTGG CCAGCGTGGA AGAGGGGGAC 
GAGACCCTGG ACTCCCCAGG AAGCCAGAGC CGAGGGACTG CCCGGCAAAA GAAGACCATG 
AAGCTGTCAC GAGCCCTCTC GGACCTAGTG AAATATACCA AGTCTGTGGG GACCCATGAC 
GTGGAGATAG AGGTGGTATC TAGCTGGCAG GTGTCGTCCT TCAGTGAGAC CAAGGCCCAT 
CAGATCCTGC AGCAGAAGCC CACCCAGTAC CTGCGCTTCA ACCAGCACCA GCTCTCGCGC 
ATATACCCCT CCTCCTACCG TGTGGACTCC AGCAACTACA ATCCACAACC CTTCTGGAAC 
GCTGGTTGCC AGATGGTTGC CCTGAACTAC CAGTCAGAGG GGCGGATGCT ACAGCTGAAC 
AGGGCCAAGT TCAGCGCCAA CGGTGACTGT GGCTACGTGC TCAAACCCCA GTGCATGTGC 
CAGGGTGTCT TCAACCCCAA CTCGGAGGAT CCCCTGCCGG GGCAGCTCAA GAAGCAGCTG 
GCCCTGAGGA TCATCAGTGG CCAGCAGCTG CCCAAACCAC GGGACTCGGT GCTGGGCGAC 
CGTGGGGAGA TCATCGACCC CTTCGTGGAG GTGGAGGTCA TTGGGCTCCC CGTGGACTGC 
AGCAAGGAGC AGACCCGAGT GGTGGACGAC AACGGATTCA ACCCCATGTG GGAGGAGACA 
CTGGTGTTCA CCGTGCACAT GCCAGAGATT GCGCTTGTAC GCTTCCTGGT CTGGGACCAT 
GACCCCATTG GACGTGACTT CATCGGCCAG AGGACACTAG CCTTCAGCAG CATAATGCCA 
GGCTACCGGC ATGTGTACCT AGAAGGGATG GAAGAGGCCT CTATCTTTGT TCATGTGGCT 
GTCAGTGACA TTAGTGGTAA GGTCAAGCAG ACTCTGGGTC TAAAAGGTCT CTTCCTCCGA 
GGCACAAAGC CAGGCTCGCT GGACAGTCAT GCTGCTGGAC AGCCCCTCCC CCGGCCCTCC 
GTTAGCCAGA GGCTCCTGCG GCGCACGGCC AGTGCCCCGA CCAAAAGCCA GAAGCCAAGT 
CGCAAGGGCT TCCCAGAGCT GGCCCTGGGC ACACAGGACG CAGGCTCCGA GGGGGCAGCT 
GATGACGTGG CACCCTCTAG CCCCAACCCA GCTCTGGAGG CCCCTACTCA AGAGAGGTCA 
GGCAGCAGCA GCCCCCGAGG TAAGGCACCA GGAGGAGAAG CAACAGAAGA GAGGACACTG 
GCACAGGTGC GGTCCCCAAA TGCCCCCGAA GGCCCTGGAC CTGCAGGGAT GGCCGCCACA 
TGCATGAAGT GTGTGGTGGG CTCCTGCGCC GGTATGGACG TTGAGGGCCT TCAAAGGGAG 
CAACAACCCA GCCCTGGGCC TGCAGGCAGC CACATGGCCA TCAGCCATCA GCCCAGGGCC 
CGGGTAGATT CACTGGGGGG CCCTTGCTGC AGCCCAAGTC CTCGAGCCAC CCCAGGGAGA 
AGCAAAGAGG CCCCCAAGGG TCCTAGGGCT CGGCGCCAGG GTCCAGGCGG CGGCTCTGTG 
TCCTCGGACT CCAGCAGCCC AGACAGCCCA GGCAGCCCCA AGGTGGCCCC CTGCCAGCCG 
GAGGGTGCTC ACAGGCAGCA GGGGGCGCTG CAGGGAGAGA TGAACGCCTT GTTCGTTCAA 
AAGCTGGAGG AGATCAGGAG TCATTCCCCC ATGTTCTCCA CCGATACTCG CCTCTTCCCC 
CTGCAGCGGC CCATCTCCCC CCTGTGCAGC CTGGAACCCA TTGCCGAGGA ACCAGCCCTG 
GGCCCTGGCC TCCCGTTACA GGCAGCTGCC CCCACAGGTC CATCTCAGGA AGGGTCCCAG 
TGCCCTGTAG GCCTGGGAGC CAAAGTGACC AGCTCCCAGC AGACATCTCT GGGAGCTTTT 
GGAACCCTCC AGCTCAGGAT TGGGGGCGGT AGGGAAAACG AAGAGCCACC CCTCAGGCCA 
CACAATGGTG GGATCTCCAG CGGGCCACGT GAGGGGACTT CTGGAAGACA GACAGACAGC 
AAGAGCCGCT CCCGGGTCCC GGGCCACTTG CCAGTGGTTA GGAGGGCCAA GAGTGAGGGC 
CAGGTGCTCT CGGAGCTCTC TCCCACACCT GCAGTGTACT CGGATGCCAC GGGCACTGAC 
AGGTTATGGC AGCGGCTGGA GCCTGGGAGC CACAGGGACA GTGTCTCCTC ATCCTCCAGC 
ATGTCCTCCA ACGACACCGT CATAGACCTA TCACTGCCCA GCCTCGGCCT CTGCCGCAGC 
CGAGAGAGCA TTCCAGGGGT CTCTCTTGGA CGCCTAACCT CACGGCCCTG CTTAGCCTCA 
GCTGCCCGTC CTGACCTGCC TCCTGTGACC AAGAGCAAAT CGAACCCCAA CCTGCGGGTT 
GCGGGTGGGC TGCCTACTGC ACCCGACGAG CTGCAGCCCC GGCCTCTTGC CCCGCGGCTG 
ACCGGCCACC ATCCACGGCC ACCGTGGCAT CACCTCACCC TGGTGGGCCT GCGGGACTGC 
CCAGTGTCTG CTAAGTCCAA GAGTCTGGGA GACCTGACAG CTGACGACTT TGCTCCCAGC 
TTTCAGGGCA GCACCAGCAG CCTGAGCTGC GGCCTAGGCT CCCTGGGTGT GGCGCATCAG 
GTGCTGGAGC CGGGAATACG GCGAGATGCT CTCACGGAGC AGCTACGCTG GCTCACCGGC 
TTCCAGCAGG CTGGGGACAT CACGTCGCCC ACCAGCTTGG GCCCAGCTGG GGACGGGTCT 
GTAGGGGGCC CCAGCTTCCT GAGGCGTTCC TCCTCTCGGA GCCAGAGCCG CGTGCGCGCT 
ATTGCCAGCC GAGCCCGCCA AGCGCAGGAG CGCCAGCAGA GGCTGAGAGG CCAGGACTCA 
CGGGGTCCCC CAGAGGAGGA GCGGGGCACC CCTGAGGGTG CCTGTTCTGT AGGCCATGAG 
GGCTGTGTGG ATGTGCCAAT GCCTGCCAAG GGAGCCCCTG AGCAGGTGTG TGGTGCTGCT 
GATGGCCAGC TGCTGCTCAG GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_006538936.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538999.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X2, mRNA.

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CloneID	OMu34258
Clone ID Related Accession (Same CDS sequence)	XM_006538936.2
Accession Version	XM_006538936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006538936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA 
CCAGGAGGAG AAGCAACAGA AGAGAGGACA CTGGCACAGG TGCGGTCCCC AAATGCCCCC 
GAAGGCCCTG GACCTGCAGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGCTCCTGC 
GCCGGTATGG ACGTTGAGGG CCTTCAAAGG GAGCAACAAC CCAGCCCTGG GCCTGCAGGC 
AGCCACATGG CCATCAGCCA TCAGCCCAGG GCCCGGGTAG ATTCACTGGG GGGCCCTTGC 
TGCAGCCCAA GTCCTCGAGC CACCCCAGGG AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG 
GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT GTGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG CCGGAGGGTG CTCACAGGCA GCAGGGGGCG 
CTGCAGGGAG AGATGAACGC CTTGTTCGTT CAAAAGCTGG AGGAGATCAG GAGTCATTCC 
CCCATGTTCT CCACCGATAC TCGCCTCTTC CCCCTGCAGC GGCCCATCTC CCCCCTGTGC 
AGCCTGGAAC CCATTGCCGA GGAACCAGCC CTGGGCCCTG GCCTCCCGTT ACAGGCAGCT 
GCCCCCACAG GTCCATCTCA GGAAGGGTCC CAGTGCCCTG TAGGCCTGGG AGCCAAAGTG 
ACCAGCTCCC AGCAGACATC TCTGGGAGCT TTTGGAACCC TCCAGCTCAG GATTGGGGGC 
GGTAGGGAAA ACGAAGAGCC ACCCCTCAGG CCACACAATG GTGGGATCTC CAGCGGGCCA 
CGTGAGGGGA CTTCTGGAAG ACAGACAGAC AGCAAGAGCC GCTCCCGGGT CCCGGGCCAC 
TTGCCAGTGG TTAGGAGGGC CAAGAGTGAG GGCCAGGTGC TCTCGGAGCT CTCTCCCACA 
CCTGCAGTGT ACTCGGATGC CACGGGCACT GACAGGTTAT GGCAGCGGCT GGAGCCTGGG 
AGCCACAGGG ACAGTGTCTC CTCATCCTCC AGCATGTCCT CCAACGACAC CGTCATAGAC 
CTATCACTGC CCAGCCTCGG CCTCTGCCGC AGCCGAGAGA GCATTCCAGG GGTCTCTCTT 
GGACGCCTAA CCTCACGGCC CTGCTTAGCC TCAGCTGCCC GTCCTGACCT GCCTCCTGTG 
ACCAAGAGCA AATCGAACCC CAACCTGCGG GTTGCGGGTG GGCTGCCTAC TGCACCCGAC 
GAGCTGCAGC CCCGGCCTCT TGCCCCGCGG CTGACCGGCC ACCATCCACG GCCACCGTGG 
CATCACCTCA CCCTGGTGGG CCTGCGGGAC TGCCCAGTGT CTGCTAAGTC CAAGAGTCTG 
GGAGACCTGA CAGCTGACGA CTTTGCTCCC AGCTTTCAGG GCAGCACCAG CAGCCTGAGC 
TGCGGCCTAG GCTCCCTGGG TGTGGCGCAT CAGGTGCTGG AGCCGGGAAT ACGGCGAGAT 
GCTCTCACGG AGCAGCTACG CTGGCTCACC GGCTTCCAGC AGGCTGGGGA CATCACGTCG 
CCCACCAGCT TGGGCCCAGC TGGGGACGGG TCTGTAGGGG GCCCCAGCTT CCTGAGGCGT 
TCCTCCTCTC GGAGCCAGAG CCGCGTGCGC GCTATTGCCA GCCGAGCCCG CCAAGCGCAG 
GAGCGCCAGC AGAGGCTGAG AGGCCAGGAC TCACGGGGTC CCCCAGAGGA GGAGCGGGGC 
ACCCCTGAGG GTGCCTGTTC TGTAGGCCAT GAGGGCTGTG TGGATGTGCC AATGCCTGCC 
AAGGGAGCCC CTGAGCAGGT GTGTGGTGCT GCTGATGGCC AGCTGCTGCT CAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538999.1
CDS	16..5235
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	XM_006538936.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538936.2

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA 
CCAGGAGGAG AAGCAACAGA AGAGAGGACA CTGGCACAGG TGCGGTCCCC AAATGCCCCC 
GAAGGCCCTG GACCTGCAGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGCTCCTGC 
GCCGGTATGG ACGTTGAGGG CCTTCAAAGG GAGCAACAAC CCAGCCCTGG GCCTGCAGGC 
AGCCACATGG CCATCAGCCA TCAGCCCAGG GCCCGGGTAG ATTCACTGGG GGGCCCTTGC 
TGCAGCCCAA GTCCTCGAGC CACCCCAGGG AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG 
GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT GTGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG CCGGAGGGTG CTCACAGGCA GCAGGGGGCG 
CTGCAGGGAG AGATGAACGC CTTGTTCGTT CAAAAGCTGG AGGAGATCAG GAGTCATTCC 
CCCATGTTCT CCACCGATAC TCGCCTCTTC CCCCTGCAGC GGCCCATCTC CCCCCTGTGC 
AGCCTGGAAC CCATTGCCGA GGAACCAGCC CTGGGCCCTG GCCTCCCGTT ACAGGCAGCT 
GCCCCCACAG GTCCATCTCA GGAAGGGTCC CAGTGCCCTG TAGGCCTGGG AGCCAAAGTG 
ACCAGCTCCC AGCAGACATC TCTGGGAGCT TTTGGAACCC TCCAGCTCAG GATTGGGGGC 
GGTAGGGAAA ACGAAGAGCC ACCCCTCAGG CCACACAATG GTGGGATCTC CAGCGGGCCA 
CGTGAGGGGA CTTCTGGAAG ACAGACAGAC AGCAAGAGCC GCTCCCGGGT CCCGGGCCAC 
TTGCCAGTGG TTAGGAGGGC CAAGAGTGAG GGCCAGGTGC TCTCGGAGCT CTCTCCCACA 
CCTGCAGTGT ACTCGGATGC CACGGGCACT GACAGGTTAT GGCAGCGGCT GGAGCCTGGG 
AGCCACAGGG ACAGTGTCTC CTCATCCTCC AGCATGTCCT CCAACGACAC CGTCATAGAC 
CTATCACTGC CCAGCCTCGG CCTCTGCCGC AGCCGAGAGA GCATTCCAGG GGTCTCTCTT 
GGACGCCTAA CCTCACGGCC CTGCTTAGCC TCAGCTGCCC GTCCTGACCT GCCTCCTGTG 
ACCAAGAGCA AATCGAACCC CAACCTGCGG GTTGCGGGTG GGCTGCCTAC TGCACCCGAC 
GAGCTGCAGC CCCGGCCTCT TGCCCCGCGG CTGACCGGCC ACCATCCACG GCCACCGTGG 
CATCACCTCA CCCTGGTGGG CCTGCGGGAC TGCCCAGTGT CTGCTAAGTC CAAGAGTCTG 
GGAGACCTGA CAGCTGACGA CTTTGCTCCC AGCTTTCAGG GCAGCACCAG CAGCCTGAGC 
TGCGGCCTAG GCTCCCTGGG TGTGGCGCAT CAGGTGCTGG AGCCGGGAAT ACGGCGAGAT 
GCTCTCACGG AGCAGCTACG CTGGCTCACC GGCTTCCAGC AGGCTGGGGA CATCACGTCG 
CCCACCAGCT TGGGCCCAGC TGGGGACGGG TCTGTAGGGG GCCCCAGCTT CCTGAGGCGT 
TCCTCCTCTC GGAGCCAGAG CCGCGTGCGC GCTATTGCCA GCCGAGCCCG CCAAGCGCAG 
GAGCGCCAGC AGAGGCTGAG AGGCCAGGAC TCACGGGGTC CCCCAGAGGA GGAGCGGGGC 
ACCCCTGAGG GTGCCTGTTC TGTAGGCCAT GAGGGCTGTG TGGATGTGCC AATGCCTGCC 
AAGGGAGCCC CTGAGCAGGT GTGTGGTGCT GCTGATGGCC AGCTGCTGCT CAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_006538940.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539003.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X8, mRNA.

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CloneID	OMu34261
Clone ID Related Accession (Same CDS sequence)	XM_006538940.2
Accession Version	XM_006538940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006538940.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGATACTCGC 
CTCTTCCCCC TGCAGCGGCC CATCTCCCCC CTGTGCAGCC TGGAACCCAT TGCCGAGGAA 
CCAGCCCTGG GCCCTGGCCT CCCGTTACAG GCAGCTGCCC CCACAGGTCC ATCTCAGGAA 
GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC AAAGTGACCA GCTCCCAGCA GACATCTCTG 
GGAGCTTTTG GAACCCTCCA GCTCAGGATT GGGGGCGGTA GGGAAAACGA AGAGCCACCC 
CTCAGGCCAC ACAATGGTGG GATCTCCAGC GGGCCACGTG AGGGGACTTC TGGAAGACAG 
ACAGACAGCA AGAGCCGCTC CCGGGTCCCG GGCCACTTGC CAGTGGTTAG GAGGGCCAAG 
AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT CCCACACCTG CAGTGTACTC GGATGCCACG 
GGCACTGACA GGTTATGGCA GCGGCTGGAG CCTGGGAGCC ACAGGGACAG TGTCTCCTCA 
TCCTCCAGCA TGTCCTCCAA CGACACCGTC ATAGACCTAT CACTGCCCAG CCTCGGCCTC 
TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC TCTCTTGGAC GCCTAACCTC ACGGCCCTGC 
TTAGCCTCAG CTGCCCGTCC TGACCTGCCT CCTGTGACCA AGAGCAAATC GAACCCCAAC 
CTGCGGGTTG CGGGTGGGCT GCCTACTGCA CCCGACGAGC TGCAGCCCCG GCCTCTTGCC 
CCGCGGCTGA CCGGCCACCA TCCACGGCCA CCGTGGCATC ACCTCACCCT GGTGGGCCTG 
CGGGACTGCC CAGTGTCTGC TAAGTCCAAG AGTCTGGGAG ACCTGACAGC TGACGACTTT 
GCTCCCAGCT TTCAGGGCAG CACCAGCAGC CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG 
GCGCATCAGG TGCTGGAGCC GGGAATACGG CGAGATGCTC TCACGGAGCA GCTACGCTGG 
CTCACCGGCT TCCAGCAGGC TGGGGACATC ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG 
GACGGGTCTG TAGGGGGCCC CAGCTTCCTG AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC 
GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC 
CAGGACTCAC GGGGTCCCCC AGAGGAGGAG CGGGGCACCC CTGAGGGTGC CTGTTCTGTA 
GGCCATGAGG GCTGTGTGGA TGTGCCAATG CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT 
GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539003.1
CDS	16..4731
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	XM_006538940.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538940.2

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGATACTCGC 
CTCTTCCCCC TGCAGCGGCC CATCTCCCCC CTGTGCAGCC TGGAACCCAT TGCCGAGGAA 
CCAGCCCTGG GCCCTGGCCT CCCGTTACAG GCAGCTGCCC CCACAGGTCC ATCTCAGGAA 
GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC AAAGTGACCA GCTCCCAGCA GACATCTCTG 
GGAGCTTTTG GAACCCTCCA GCTCAGGATT GGGGGCGGTA GGGAAAACGA AGAGCCACCC 
CTCAGGCCAC ACAATGGTGG GATCTCCAGC GGGCCACGTG AGGGGACTTC TGGAAGACAG 
ACAGACAGCA AGAGCCGCTC CCGGGTCCCG GGCCACTTGC CAGTGGTTAG GAGGGCCAAG 
AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT CCCACACCTG CAGTGTACTC GGATGCCACG 
GGCACTGACA GGTTATGGCA GCGGCTGGAG CCTGGGAGCC ACAGGGACAG TGTCTCCTCA 
TCCTCCAGCA TGTCCTCCAA CGACACCGTC ATAGACCTAT CACTGCCCAG CCTCGGCCTC 
TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC TCTCTTGGAC GCCTAACCTC ACGGCCCTGC 
TTAGCCTCAG CTGCCCGTCC TGACCTGCCT CCTGTGACCA AGAGCAAATC GAACCCCAAC 
CTGCGGGTTG CGGGTGGGCT GCCTACTGCA CCCGACGAGC TGCAGCCCCG GCCTCTTGCC 
CCGCGGCTGA CCGGCCACCA TCCACGGCCA CCGTGGCATC ACCTCACCCT GGTGGGCCTG 
CGGGACTGCC CAGTGTCTGC TAAGTCCAAG AGTCTGGGAG ACCTGACAGC TGACGACTTT 
GCTCCCAGCT TTCAGGGCAG CACCAGCAGC CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG 
GCGCATCAGG TGCTGGAGCC GGGAATACGG CGAGATGCTC TCACGGAGCA GCTACGCTGG 
CTCACCGGCT TCCAGCAGGC TGGGGACATC ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG 
GACGGGTCTG TAGGGGGCCC CAGCTTCCTG AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC 
GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC 
CAGGACTCAC GGGGTCCCCC AGAGGAGGAG CGGGGCACCC CTGAGGGTGC CTGTTCTGTA 
GGCCATGAGG GCTGTGTGGA TGTGCCAATG CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT 
GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250268.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248570.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X1, mRNA.

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CloneID	OMu74423
Clone ID Related Accession (Same CDS sequence)	XM_011250268.1
Accession Version	XM_011250268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA 
CCAGGAGGAG AAGCAACAGA AGAGAGGACA CTGGCACAGG TGCGGTCCCC AAATGCCCCC 
GAAGGCCCTG GACCTGCAGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGCTCCTGC 
GCCGGTATGG ACGTTGAGGG CCTTCAAAGG GAGCAACAAC CCAGCCCTGG GCCTGCAGGC 
AGCCACATGG CCATCAGCCA TCAGCCCAGG GCCCGGGTAG ATTCACTGGG GGGCCCTTGC 
TGCAGCCCAA GTCCTCGAGC CACCCCAGGG AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG 
GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT GTGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG CCGGAGGGTG CTCACAGGCA GCAGGGGGCG 
CTGCAGGGAG AGATGAACGC CTTGTTCGTT CAAAAGCTGG AGGAGATCAG GAGTCATTCC 
CCCATGTTCT CCACCGATAC TCGCCTCTTC CCCCTGCAGC GGCCCATCTC CCCCCTGTGC 
AGCCTGGAAC CCATTGCCGA GGAACCAGCC CTGGGCCCTG GCCTCCCGTT ACAGGCAGCT 
GCCCCCACAG GTCCATCTCA GGAAGGGTCC CAGTGCCCTG TAGGCCTGGG AGCCAAAGTG 
ACCAGCTCCC AGCAGACATC TCTGGGAGCT TTTGGAACCC TCCAGCTCAG GATTGGGGGC 
GGTAGGGAAA ACGAAGAGCC ACCCCTCAGG CCACACAATG GTGGGATCTC CAGCGGGCCA 
CGTGAGGGGA CTTCTGGAAG ACAGACAGAC AGCAAGAGCC GCTCCCGGGT CCCGGGCCAC 
TTGCCAGTGG TTAGGAGGGC CAAGAGTGAG GGCCAGGTGC TCTCGGAGCT CTCTCCCACA 
CCTGCAGTGT ACTCGGATGC CACGGGCACT GACAGGTTAT GGCAGCGGCT GGAGCCTGGG 
AGCCACAGGG ACAGTGTCTC CTCATCCTCC AGCATGTCCT CCAACGACAC CGTCATAGAC 
CTATCACTGC CCAGCCTCGG CCTCTGCCGC AGCCGAGAGA GCATTCCAGG GGTCTCTCTT 
GGACGCCTAA CCTCACGGCC CTGCTTAGCC TCAGCTGCCC GTCCTGACCT GCCTCCTGTG 
ACCAAGAGCA AATCGAACCC CAACCTGCGG GTTGCGGGTG GGCTGCCTAC TGCACCCGAC 
GAGCTGCAGC CCCGGCCTCT TGCCCCGCGG CTGACCGGCC ACCATCCACG GCCACCGTGG 
CATCACCTCA CCCTGGTGGG CCTGCGGGAC TGCCCAGTGT CTGCTAAGTC CAAGAGTCTG 
GGAGACCTGA CAGCTGACGA CTTTGCTCCC AGCTTTCAGG GCAGCACCAG CAGCCTGAGC 
TGCGGCCTAG GCTCCCTGGG TGTGGCGCAT CAGGTGCTGG AGCCGGGAAT ACGGCGAGAT 
GCTCTCACGG AGCAGCTACG CTGGCTCACC GGCTTCCAGC AGGCTGGGGA CATCACGTCG 
CCCACCAGCT TGGGCCCAGC TGGGGACGGG TCTGTAGGGG GCCCCAGCTT CCTGAGGCGT 
TCCTCCTCTC GGAGCCAGAG CCGCGTGCGC GCTATTGCCA GCCGAGCCCG CCAAGCGCAG 
GAGCGCCAGC AGAGGCTGAG AGGCCAGGAC TCACGGGGTC CCCCAGAGGA GGAGCGGGGC 
ACCCCTGAGG GTGCCTGTTC TGTAGGCCAT GAGGGCTGTG TGGATGTGCC AATGCCTGCC 
AAGGGAGCCC CTGAGCAGGT GTGTGGTGCT GCTGATGGCC AGCTGCTGCT CAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248570.1
CDS	16..5295
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	XM_011250268.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250268.1

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA 
CCAGGAGGAG AAGCAACAGA AGAGAGGACA CTGGCACAGG TGCGGTCCCC AAATGCCCCC 
GAAGGCCCTG GACCTGCAGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGCTCCTGC 
GCCGGTATGG ACGTTGAGGG CCTTCAAAGG GAGCAACAAC CCAGCCCTGG GCCTGCAGGC 
AGCCACATGG CCATCAGCCA TCAGCCCAGG GCCCGGGTAG ATTCACTGGG GGGCCCTTGC 
TGCAGCCCAA GTCCTCGAGC CACCCCAGGG AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG 
GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT GTGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG CCGGAGGGTG CTCACAGGCA GCAGGGGGCG 
CTGCAGGGAG AGATGAACGC CTTGTTCGTT CAAAAGCTGG AGGAGATCAG GAGTCATTCC 
CCCATGTTCT CCACCGATAC TCGCCTCTTC CCCCTGCAGC GGCCCATCTC CCCCCTGTGC 
AGCCTGGAAC CCATTGCCGA GGAACCAGCC CTGGGCCCTG GCCTCCCGTT ACAGGCAGCT 
GCCCCCACAG GTCCATCTCA GGAAGGGTCC CAGTGCCCTG TAGGCCTGGG AGCCAAAGTG 
ACCAGCTCCC AGCAGACATC TCTGGGAGCT TTTGGAACCC TCCAGCTCAG GATTGGGGGC 
GGTAGGGAAA ACGAAGAGCC ACCCCTCAGG CCACACAATG GTGGGATCTC CAGCGGGCCA 
CGTGAGGGGA CTTCTGGAAG ACAGACAGAC AGCAAGAGCC GCTCCCGGGT CCCGGGCCAC 
TTGCCAGTGG TTAGGAGGGC CAAGAGTGAG GGCCAGGTGC TCTCGGAGCT CTCTCCCACA 
CCTGCAGTGT ACTCGGATGC CACGGGCACT GACAGGTTAT GGCAGCGGCT GGAGCCTGGG 
AGCCACAGGG ACAGTGTCTC CTCATCCTCC AGCATGTCCT CCAACGACAC CGTCATAGAC 
CTATCACTGC CCAGCCTCGG CCTCTGCCGC AGCCGAGAGA GCATTCCAGG GGTCTCTCTT 
GGACGCCTAA CCTCACGGCC CTGCTTAGCC TCAGCTGCCC GTCCTGACCT GCCTCCTGTG 
ACCAAGAGCA AATCGAACCC CAACCTGCGG GTTGCGGGTG GGCTGCCTAC TGCACCCGAC 
GAGCTGCAGC CCCGGCCTCT TGCCCCGCGG CTGACCGGCC ACCATCCACG GCCACCGTGG 
CATCACCTCA CCCTGGTGGG CCTGCGGGAC TGCCCAGTGT CTGCTAAGTC CAAGAGTCTG 
GGAGACCTGA CAGCTGACGA CTTTGCTCCC AGCTTTCAGG GCAGCACCAG CAGCCTGAGC 
TGCGGCCTAG GCTCCCTGGG TGTGGCGCAT CAGGTGCTGG AGCCGGGAAT ACGGCGAGAT 
GCTCTCACGG AGCAGCTACG CTGGCTCACC GGCTTCCAGC AGGCTGGGGA CATCACGTCG 
CCCACCAGCT TGGGCCCAGC TGGGGACGGG TCTGTAGGGG GCCCCAGCTT CCTGAGGCGT 
TCCTCCTCTC GGAGCCAGAG CCGCGTGCGC GCTATTGCCA GCCGAGCCCG CCAAGCGCAG 
GAGCGCCAGC AGAGGCTGAG AGGCCAGGAC TCACGGGGTC CCCCAGAGGA GGAGCGGGGC 
ACCCCTGAGG GTGCCTGTTC TGTAGGCCAT GAGGGCTGTG TGGATGTGCC AATGCCTGCC 
AAGGGAGCCC CTGAGCAGGT GTGTGGTGCT GCTGATGGCC AGCTGCTGCT CAGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250272.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248574.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X6, mRNA.

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CloneID	OMu74426
Clone ID Related Accession (Same CDS sequence)	XM_011250272.1
Accession Version	XM_011250272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGATACTCGC 
CTCTTCCCCC TGCAGCGGCC CATCTCCCCC CTGTGCAGCC TGGAACCCAT TGCCGAGGAA 
CCAGCCCTGG GCCCTGGCCT CCCGTTACAG GCAGCTGCCC CCACAGGTCC ATCTCAGGAA 
GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC AAAGTGACCA GCTCCCAGCA GACATCTCTG 
GGAGCTTTTG GAACCCTCCA GCTCAGGATT GGGGGCGGTA GGGAAAACGA AGAGCCACCC 
CTCAGGCCAC ACAATGGTGG GATCTCCAGC GGGCCACGTG AGGGGACTTC TGGAAGACAG 
ACAGACAGCA AGAGCCGCTC CCGGGTCCCG GGCCACTTGC CAGTGGTTAG GAGGGCCAAG 
AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT CCCACACCTG CAGTGTACTC GGATGCCACG 
GGCACTGACA GGTTATGGCA GCGGCTGGAG CCTGGGAGCC ACAGGGACAG TGTCTCCTCA 
TCCTCCAGCA TGTCCTCCAA CGACACCGTC ATAGACCTAT CACTGCCCAG CCTCGGCCTC 
TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC TCTCTTGGAC GCCTAACCTC ACGGCCCTGC 
TTAGCCTCAG CTGCCCGTCC TGACCTGCCT CCTGTGACCA AGAGCAAATC GAACCCCAAC 
CTGCGGGTTG CGGGTGGGCT GCCTACTGCA CCCGACGAGC TGCAGCCCCG GCCTCTTGCC 
CCGCGGCTGA CCGGCCACCA TCCACGGCCA CCGTGGCATC ACCTCACCCT GGTGGGCCTG 
CGGGACTGCC CAGTGTCTGC TAAGTCCAAG AGTCTGGGAG ACCTGACAGC TGACGACTTT 
GCTCCCAGCT TTCAGGGCAG CACCAGCAGC CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG 
GCGCATCAGG TGCTGGAGCC GGGAATACGG CGAGATGCTC TCACGGAGCA GCTACGCTGG 
CTCACCGGCT TCCAGCAGGC TGGGGACATC ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG 
GACGGGTCTG TAGGGGGCCC CAGCTTCCTG AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC 
GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC 
CAGGACTCAC GGGGTCCCCC AGAGGAGGAG CGGGGCACCC CTGAGGGTGC CTGTTCTGTA 
GGCCATGAGG GCTGTGTGGA TGTGCCAATG CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT 
GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248574.1
CDS	16..4791
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRDTRLFPLQRPISPLCSLEPIAEEPALGPGLPLQAAAPTGPSQEGSQCPVGLGAKVTSSQQTSLGAFGTLQLRIGGGRENEEPPLRPHNGGISSGPREGTSGRQTDSKSRSRVPGHLPVVRRAKSEGQVLSELSPTPAVYSDATGTDRLWQRLEPGSHRDSVSSSSSMSSNDTVIDLSLPSLGLCRSRESIPGVSLGRLTSRPCLASAARPDLPPVTKSKSNPNLRVAGGLPTAPDELQPRPLAPRLTGHHPRPPWHHLTLVGLRDCPVSAKSKSLGDLTADDFAPSFQGSTSSLSCGLGSLGVAHQVLEPGIRRDALTEQLRWLTGFQQAGDITSPTSLGPAGDGSVGGPSFLRRSSSRSQSRVRAIASRARQAQERQQRLRGQDSRGPPEEERGTPEGACSVGHEGCVDVPMPAKGAPEQVCGAADGQLLLRL

Target ORF information:

RefSeq Version	XM_011250272.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250272.1

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGATACTCGC 
CTCTTCCCCC TGCAGCGGCC CATCTCCCCC CTGTGCAGCC TGGAACCCAT TGCCGAGGAA 
CCAGCCCTGG GCCCTGGCCT CCCGTTACAG GCAGCTGCCC CCACAGGTCC ATCTCAGGAA 
GGGTCCCAGT GCCCTGTAGG CCTGGGAGCC AAAGTGACCA GCTCCCAGCA GACATCTCTG 
GGAGCTTTTG GAACCCTCCA GCTCAGGATT GGGGGCGGTA GGGAAAACGA AGAGCCACCC 
CTCAGGCCAC ACAATGGTGG GATCTCCAGC GGGCCACGTG AGGGGACTTC TGGAAGACAG 
ACAGACAGCA AGAGCCGCTC CCGGGTCCCG GGCCACTTGC CAGTGGTTAG GAGGGCCAAG 
AGTGAGGGCC AGGTGCTCTC GGAGCTCTCT CCCACACCTG CAGTGTACTC GGATGCCACG 
GGCACTGACA GGTTATGGCA GCGGCTGGAG CCTGGGAGCC ACAGGGACAG TGTCTCCTCA 
TCCTCCAGCA TGTCCTCCAA CGACACCGTC ATAGACCTAT CACTGCCCAG CCTCGGCCTC 
TGCCGCAGCC GAGAGAGCAT TCCAGGGGTC TCTCTTGGAC GCCTAACCTC ACGGCCCTGC 
TTAGCCTCAG CTGCCCGTCC TGACCTGCCT CCTGTGACCA AGAGCAAATC GAACCCCAAC 
CTGCGGGTTG CGGGTGGGCT GCCTACTGCA CCCGACGAGC TGCAGCCCCG GCCTCTTGCC 
CCGCGGCTGA CCGGCCACCA TCCACGGCCA CCGTGGCATC ACCTCACCCT GGTGGGCCTG 
CGGGACTGCC CAGTGTCTGC TAAGTCCAAG AGTCTGGGAG ACCTGACAGC TGACGACTTT 
GCTCCCAGCT TTCAGGGCAG CACCAGCAGC CTGAGCTGCG GCCTAGGCTC CCTGGGTGTG 
GCGCATCAGG TGCTGGAGCC GGGAATACGG CGAGATGCTC TCACGGAGCA GCTACGCTGG 
CTCACCGGCT TCCAGCAGGC TGGGGACATC ACGTCGCCCA CCAGCTTGGG CCCAGCTGGG 
GACGGGTCTG TAGGGGGCCC CAGCTTCCTG AGGCGTTCCT CCTCTCGGAG CCAGAGCCGC 
GTGCGCGCTA TTGCCAGCCG AGCCCGCCAA GCGCAGGAGC GCCAGCAGAG GCTGAGAGGC 
CAGGACTCAC GGGGTCCCCC AGAGGAGGAG CGGGGCACCC CTGAGGGTGC CTGTTCTGTA 
GGCCATGAGG GCTGTGTGGA TGTGCCAATG CCTGCCAAGG GAGCCCCTGA GCAGGTGTGT 
GGTGCTGCTG ATGGCCAGCT GCTGCTCAGG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250274.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248576.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X10, mRNA.

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CloneID	OMu74428
Clone ID Related Accession (Same CDS sequence)	XM_011250274.2
Accession Version	XM_011250274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA 
CCAGGAGGAG AAGCAACAGA AGAGAGGACA CTGGCACAGG TGCGGTCCCC AAATGCCCCC 
GAAGGCCCTG GACCTGCAGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGCTCCTGC 
GCCGGTATGG ACGTTGAGGG CCTTCAAAGG GAGCAACAAC CCAGCCCTGG GCCTGCAGGC 
AGCCACATGG CCATCAGCCA TCAGCCCAGG GCCCGGGTAG ATTCACTGGG GGGCCCTTGC 
TGCAGCCCAA GTCCTCGAGC CACCCCAGGG AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG 
GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT GTGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG CCGGAGGGTG CTCACAGGCA GCAGGGGGCG 
CTGCAGGGAG AGATGAACGC CTTGTTCGTT CAAAAGCTGG AGGAGATCAG GAGTCATTCC 
CCCATGTTCT CCACCGTTAG GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248576.1
CDS	16..4002
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTVRD

Target ORF information:

RefSeq Version	XM_011250274.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250274.2

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA 
CCAGGAGGAG AAGCAACAGA AGAGAGGACA CTGGCACAGG TGCGGTCCCC AAATGCCCCC 
GAAGGCCCTG GACCTGCAGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGCTCCTGC 
GCCGGTATGG ACGTTGAGGG CCTTCAAAGG GAGCAACAAC CCAGCCCTGG GCCTGCAGGC 
AGCCACATGG CCATCAGCCA TCAGCCCAGG GCCCGGGTAG ATTCACTGGG GGGCCCTTGC 
TGCAGCCCAA GTCCTCGAGC CACCCCAGGG AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG 
GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT GTGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG CCGGAGGGTG CTCACAGGCA GCAGGGGGCG 
CTGCAGGGAG AGATGAACGC CTTGTTCGTT CAAAAGCTGG AGGAGATCAG GAGTCATTCC 
CCCATGTTCT CCACCGTTAG GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250275.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248577.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu74429
Clone ID Related Accession (Same CDS sequence)	XM_011250275.1 , XM_011250275.2
Accession Version	XM_011250275.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGTGGGCT 
TCTCTATGTC CCAAGTCTAC TTTTAGAAGC TCAGCCTTGT TAGGAGTCTC AAAGGTACCC 
GGCGCCGCGG GACGGTGTAG CCCGCCGCCC CACCTGCATG CCAGGCCGCG CCCATTAGCA 
TCCGTGATAC TTGGTAGCAT TTTGGAGACT GTCAGCTCAT GTGACATGGA TCATGACACG 
TTTCTGGGGT CCCCTGGGCC ATCCTCAGCC AGAGAGTGGC ATTTACGAGG CAGATCCAGG 
ATTCAGCCAA AGCCAAGGAC CTATCCTCCT ATCCTGTCCT CACCCTGTGC ACCTTCTGCC 
CTTCCCCATG ACTGTAGGTC TCTCTGCACA GCCTTGCAGC TCCTCTGCCG TCCTGTCCTC 
TCTACCTGCC TACACTGTCA GTTACTGGCC CCTCTAGCTC TCTCCTCTGT GTATGCTAGG 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248577.1
CDS	16..3621
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKWASLCPKSTFRSSALLGVSKVPGAAGRCSPPPHLHARPRPLASVILGSILETVSSCDMDHDTFLGSPGPSSAREWHLRGRSRIQPKPRTYPPILSSPCAPSALPHDCRSLCTALQLLCRPVLSTCLHCQLLAPLALSSVYARI

Target ORF information:

RefSeq Version	XM_011250275.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250275.1

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGTGGGCT 
TCTCTATGTC CCAAGTCTAC TTTTAGAAGC TCAGCCTTGT TAGGAGTCTC AAAGGTACCC 
GGCGCCGCGG GACGGTGTAG CCCGCCGCCC CACCTGCATG CCAGGCCGCG CCCATTAGCA 
TCCGTGATAC TTGGTAGCAT TTTGGAGACT GTCAGCTCAT GTGACATGGA TCATGACACG 
TTTCTGGGGT CCCCTGGGCC ATCCTCAGCC AGAGAGTGGC ATTTACGAGG CAGATCCAGG 
ATTCAGCCAA AGCCAAGGAC CTATCCTCCT ATCCTGTCCT CACCCTGTGC ACCTTCTGCC 
CTTCCCCATG ACTGTAGGTC TCTCTGCACA GCCTTGCAGC TCCTCTGCCG TCCTGTCCTC 
TCTACCTGCC TACACTGTCA GTTACTGGCC CCTCTAGCTC TCTCCTCTGT GTATGCTAGG 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_011250276.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248578.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X13, mRNA.

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CloneID	OMu74430
Clone ID Related Accession (Same CDS sequence)	XM_011250276.1 , XM_011250276.2
Accession Version	XM_011250276.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGTTAGGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248578.1
CDS	16..3498
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGSREPSPVMEIHHGAPVYTAGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRVRD

Target ORF information:

RefSeq Version	XM_011250276.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250276.1

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGTAGTCGT GAGCCGAGCC CAGTGATGGA GATACACCAC 
GGCGCTCCTG TGTACACCGC AGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGTTAGGGAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plch2 ( XM_017320247.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175736.1 Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X11, mRNA.

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CloneID	OMu81007
Clone ID Related Accession (Same CDS sequence)	XM_017320247.1
Accession Version	XM_017320247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA 
CCAGGAGGAG AAGCAACAGA AGAGAGGACA CTGGCACAGG TGCGGTCCCC AAATGCCCCC 
GAAGGCCCTG GACCTGCAGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGCTCCTGC 
GCCGGTATGG ACGTTGAGGG CCTTCAAAGG GAGCAACAAC CCAGCCCTGG GCCTGCAGGC 
AGCCACATGG CCATCAGCCA TCAGCCCAGG GCCCGGGTAG ATTCACTGGG GGGCCCTTGC 
TGCAGCCCAA GTCCTCGAGC CACCCCAGGG AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG 
GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT GTGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG CCGGAGGGTG CTCACAGGCA GCAGGGGGCG 
CTGCAGGGAG AGATGAACGC CTTGTTCGTT CAAAAGCTGG AGGAGATCAG GAGTCATTCC 
CCCATGTTCT CCACCGTTAG GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175736.1
CDS	16..3942
Translation	MTSRSALCFGCVEQPRDVGSALGPAPSWSEAPATQRRGGRLRVHLCRKSTLVAADAGRVLAPLSSRLPVSARCACPRFHVAPRSTRSAAEGPPAPSQRGAGRGCGAGGTSDSALLARRLHGAQCHSAERAARTVRRGPPALAKRCPRNLSLSQSQATDHPSPRPRGHLAAVNAETMDAGAAPQKHMERCMSAMQEGTQMVKLRGSSKGLVRFYYLDEHRSCLRWRPSRKNEKAKISIDSIQEVSEGRQSEIFQRYPDSSFDPNCCFSIYHGSHRESLDLVSPSSEEARTWVTGLRYLMAGISDEDSLARRQRTRDQWLKQTFDEADKNGDGSLSISEVLQLLHKLNVNLPRQRVKQMFREADTDDHQGTLGFEEFCAFYKMMSTRRDLYLLMLTYSNHKDHLDASDLQRFLEVEQKMNGVTLESCQNIIEQFEPCLENKSKGMLGIDGFTNYTRSPAGDIFNPEHNRVHQDMTQPLSHYFITSSHNTYLVGDQLMSQSRVDMYAWVLQAGCRCVEVDCWDGPDGEPIVHHGYTLTSKILFKDVIETINKYAFIKNEYPVILSIENHCSVVQQKKMAQYLTDILGDKLDLSSVSSEDATMLPSPQMLKGKILVKGKKLPANISEDAEEGEVSDEDSADEMEDDCKLLNGDASTNRKRVENIAKKKLDSLIKESKIRDCEDPNDFSVSTLSPSGKLGRKAEAKKGQSKVEEDVEAGEDSGVSRQNSRLFMSSFSKRKKKGSKIKKVASVEEGDETLDSPGSQSRGTARQKKTMKLSRALSDLVKYTKSVGTHDVEIEVVSSWQVSSFSETKAHQILQQKPTQYLRFNQHQLSRIYPSSYRVDSSNYNPQPFWNAGCQMVALNYQSEGRMLQLNRAKFSANGDCGYVLKPQCMCQGVFNPNSEDPLPGQLKKQLALRIISGQQLPKPRDSVLGDRGEIIDPFVEVEVIGLPVDCSKEQTRVVDDNGFNPMWEETLVFTVHMPEIALVRFLVWDHDPIGRDFIGQRTLAFSSIMPGYRHVYLEGMEEASIFVHVAVSDISGKVKQTLGLKGLFLRGTKPGSLDSHAAGQPLPRPSVSQRLLRRTASAPTKSQKPSRKGFPELALGTQDAGSEGAADDVAPSSPNPALEAPTQERSGSSSPRGKAPGGEATEERTLAQVRSPNAPEGPGPAGMAATCMKCVVGSCAGMDVEGLQREQQPSPGPAGSHMAISHQPRARVDSLGGPCCSPSPRATPGRSKEAPKGPRARRQGPGGGSVSSDSSSPDSPGSPKVAPCQPEGAHRQQGALQGEMNALFVQKLEEIRSHSPMFSTVRD

Target ORF information:

RefSeq Version	XM_017320247.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phospholipase C, eta 2 (Plch2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320247.1

ORF Insert Sequence:

ATGACCTCCC GCTCAGCTCT TTGCTTTGGC TGCGTGGAGC AGCCTCGAGA TGTGGGAAGT 
GCTCTCGGCC CCGCCCCCTC CTGGTCCGAG GCTCCGGCCA CGCAGAGGCG AGGGGGGAGG 
CTGCGGGTGC ATCTTTGCAG AAAGAGTACT TTGGTTGCGG CGGACGCGGG GCGGGTGCTG 
GCCCCGCTGT CCTCTCGGCT CCCAGTCTCT GCGCGTTGCG CTTGCCCGCG GTTCCACGTC 
GCGCCCCGCT CTACGCGCAG CGCAGCCGAG GGCCCCCCGG CCCCCTCCCA GCGCGGGGCG 
GGCAGGGGGT GTGGTGCGGG CGGCACGAGT GACAGCGCGC TCCTCGCGCG GCGCCTCCAC 
GGGGCTCAGT GTCACAGCGC CGAACGCGCT GCGCGCACAG TCCGCCGCGG GCCGCCAGCC 
CTGGCGAAGC GATGTCCTCG GAACCTTAGC CTCAGTCAGA GCCAGGCCAC TGACCACCCG 
AGTCCGCGCC CTCGTGGCCA CCTCGCTGCT GTCAACGCGG AGACAATGGA CGCGGGAGCC 
GCCCCGCAGA AGCACATGGA GCGATGCATG AGTGCCATGC AAGAGGGGAC CCAGATGGTG 
AAGCTCCGTG GCAGCTCCAA GGGATTGGTC CGCTTCTACT ACCTGGATGA GCACCGCTCC 
TGCCTCCGAT GGCGTCCCTC CCGCAAGAAC GAGAAAGCCA AGATTTCCAT CGACTCCATC 
CAGGAGGTGA GTGAGGGCCG GCAGTCTGAA ATCTTCCAGC GCTACCCGGA CAGCAGCTTT 
GACCCCAACT GCTGCTTCAG CATCTACCAT GGCAGCCACA GAGAGTCGCT GGACTTGGTC 
TCACCCAGCA GTGAAGAGGC ACGTACCTGG GTCACCGGCC TTCGCTACCT CATGGCTGGC 
ATCAGCGATG AAGACAGCCT AGCCCGTCGC CAGCGTACCA GGGACCAGTG GCTGAAGCAG 
ACGTTTGATG AGGCTGACAA GAACGGGGAC GGCAGCCTGA GCATCAGTGA GGTTCTGCAG 
CTGCTACATA AACTCAACGT GAACCTGCCC CGGCAGAGGG TGAAACAGAT GTTCAGGGAG 
GCGGACACAG ATGACCATCA GGGGACATTA GGCTTTGAGG AATTCTGCGC CTTCTACAAG 
ATGATGTCTA CCCGCCGAGA CCTCTACCTG CTCATGCTGA CCTACAGCAA CCATAAGGAC 
CACTTGGATG CCTCCGACCT GCAGCGCTTT CTGGAGGTGG AGCAGAAGAT GAACGGTGTG 
ACCCTGGAGA GCTGTCAGAA CATCATTGAG CAGTTTGAGC CTTGCCTGGA AAATAAGAGC 
AAGGGGATGC TGGGGATTGA TGGCTTTACC AACTACACCC GGAGCCCCGC CGGTGACATC 
TTCAACCCTG AGCACAACAG AGTGCACCAA GACATGACGC AGCCGCTGAG TCACTACTTC 
ATCACCTCGT CCCACAACAC CTACCTCGTG GGTGACCAGC TCATGTCCCA GTCTCGGGTG 
GACATGTATG CCTGGGTCCT GCAGGCCGGC TGCCGCTGTG TGGAGGTGGA CTGCTGGGAC 
GGGCCCGATG GAGAACCCAT TGTCCATCAT GGCTACACTC TGACCTCCAA GATCCTTTTC 
AAAGATGTCA TCGAAACCAT CAACAAATAC GCCTTCATCA AGAATGAGTA CCCAGTGATC 
CTGTCCATTG AGAACCACTG TAGTGTTGTT CAGCAGAAGA AGATGGCCCA GTATCTGACT 
GACATCCTCG GGGACAAGCT GGACCTCTCC TCAGTGAGCA GCGAAGATGC CACCATGCTT 
CCATCTCCAC AGATGCTCAA GGGCAAGATC CTGGTGAAGG GGAAGAAGCT CCCTGCCAAT 
ATCAGTGAGG ATGCAGAAGA AGGCGAAGTA TCTGATGAGG ACAGTGCTGA TGAGATGGAG 
GATGACTGCA AGCTCCTCAA TGGGGATGCC TCCACCAATC GGAAGCGTGT GGAAAACATT 
GCAAAGAAGA AGCTGGATTC TCTGATCAAG GAGTCAAAGA TCCGTGACTG TGAAGACCCC 
AATGACTTCT CTGTGTCCAC ACTATCCCCC TCTGGAAAGC TTGGGCGCAA AGCAGAGGCC 
AAGAAGGGTC AGAGCAAGGT TGAGGAAGAC GTGGAGGCCG GGGAAGACAG CGGGGTGAGC 
AGGCAGAATA GCCGCCTCTT CATGAGCAGC TTCTCCAAGC GCAAGAAAAA AGGCAGCAAG 
ATAAAGAAGG TGGCCAGCGT GGAAGAGGGG GACGAGACCC TGGACTCCCC AGGAAGCCAG 
AGCCGAGGGA CTGCCCGGCA AAAGAAGACC ATGAAGCTGT CACGAGCCCT CTCGGACCTA 
GTGAAATATA CCAAGTCTGT GGGGACCCAT GACGTGGAGA TAGAGGTGGT ATCTAGCTGG 
CAGGTGTCGT CCTTCAGTGA GACCAAGGCC CATCAGATCC TGCAGCAGAA GCCCACCCAG 
TACCTGCGCT TCAACCAGCA CCAGCTCTCG CGCATATACC CCTCCTCCTA CCGTGTGGAC 
TCCAGCAACT ACAATCCACA ACCCTTCTGG AACGCTGGTT GCCAGATGGT TGCCCTGAAC 
TACCAGTCAG AGGGGCGGAT GCTACAGCTG AACAGGGCCA AGTTCAGCGC CAACGGTGAC 
TGTGGCTACG TGCTCAAACC CCAGTGCATG TGCCAGGGTG TCTTCAACCC CAACTCGGAG 
GATCCCCTGC CGGGGCAGCT CAAGAAGCAG CTGGCCCTGA GGATCATCAG TGGCCAGCAG 
CTGCCCAAAC CACGGGACTC GGTGCTGGGC GACCGTGGGG AGATCATCGA CCCCTTCGTG 
GAGGTGGAGG TCATTGGGCT CCCCGTGGAC TGCAGCAAGG AGCAGACCCG AGTGGTGGAC 
GACAACGGAT TCAACCCCAT GTGGGAGGAG ACACTGGTGT TCACCGTGCA CATGCCAGAG 
ATTGCGCTTG TACGCTTCCT GGTCTGGGAC CATGACCCCA TTGGACGTGA CTTCATCGGC 
CAGAGGACAC TAGCCTTCAG CAGCATAATG CCAGGCTACC GGCATGTGTA CCTAGAAGGG 
ATGGAAGAGG CCTCTATCTT TGTTCATGTG GCTGTCAGTG ACATTAGTGG TAAGGTCAAG 
CAGACTCTGG GTCTAAAAGG TCTCTTCCTC CGAGGCACAA AGCCAGGCTC GCTGGACAGT 
CATGCTGCTG GACAGCCCCT CCCCCGGCCC TCCGTTAGCC AGAGGCTCCT GCGGCGCACG 
GCCAGTGCCC CGACCAAAAG CCAGAAGCCA AGTCGCAAGG GCTTCCCAGA GCTGGCCCTG 
GGCACACAGG ACGCAGGCTC CGAGGGGGCA GCTGATGACG TGGCACCCTC TAGCCCCAAC 
CCAGCTCTGG AGGCCCCTAC TCAAGAGAGG TCAGGCAGCA GCAGCCCCCG AGGTAAGGCA 
CCAGGAGGAG AAGCAACAGA AGAGAGGACA CTGGCACAGG TGCGGTCCCC AAATGCCCCC 
GAAGGCCCTG GACCTGCAGG GATGGCCGCC ACATGCATGA AGTGTGTGGT GGGCTCCTGC 
GCCGGTATGG ACGTTGAGGG CCTTCAAAGG GAGCAACAAC CCAGCCCTGG GCCTGCAGGC 
AGCCACATGG CCATCAGCCA TCAGCCCAGG GCCCGGGTAG ATTCACTGGG GGGCCCTTGC 
TGCAGCCCAA GTCCTCGAGC CACCCCAGGG AGAAGCAAAG AGGCCCCCAA GGGTCCTAGG 
GCTCGGCGCC AGGGTCCAGG CGGCGGCTCT GTGTCCTCGG ACTCCAGCAG CCCAGACAGC 
CCAGGCAGCC CCAAGGTGGC CCCCTGCCAG CCGGAGGGTG CTCACAGGCA GCAGGGGGCG 
CTGCAGGGAG AGATGAACGC CTTGTTCGTT CAAAAGCTGG AGGAGATCAG GAGTCATTCC 
CCCATGTTCT CCACCGTTAG GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Plch2
Entrez Gene ID	269615
Full Name	phospholipase C, eta 2
Synonyms	A930027K05Rik,PLCeta2,Plc-eta2,Plcl4
General protein information	Preferred Names phospholipase C, eta 2 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 phosphoinositide phospholipase C-eta-2 phosphoinositide phospholipase C-like 4 phospholipase C-like protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 E2

Homo sapiens (human)	PLCH2	NP_055453.2
Pan troglodytes (chimpanzee)	PLCH2	XP_003307816.2
Mus musculus (house mouse)	Plch2	NP_001106831.1
Oryza sativa (rice)	Os03g0289300	NP_001049792.2
	PLCH2	XP_001085424.2
Arabidopsis thaliana (thale cress)	AT2G40116	NP_850327.2
Xenopus tropicalis (tropical clawed frog)	LOC100487353	XP_002936604.2
Canis lupus familiaris (dog)	PLCH2	XP_005620480.1
Bos taurus (cattle)	PLCH2	XP_005217252.1
Rattus norvegicus (Norway rat)	Plch2	XP_006239682.1
Danio rerio (zebrafish)	plch2	XP_689411.6
Gallus gallus (chicken)	PLCH2	XP_425738.3
Oryza sativa (rice)	Os07g0694000	NP_001060735.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Plch2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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REQUEST A QUOTE

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