ptk7 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ptk7
Entrez Gene ID	100487595
Full Name	protein tyrosine kinase 7 (inactive)
Synonyms	cck-4,cck4,klg,ptk-7
General protein information	Preferred Names protein tyrosine kinase 7 (inactive) Names inactive tyrosine-protein kinase 7 PTK7 protein tyrosine kinase 7
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002944431.4	XP_002944477.4	inactive tyrosine-protein kinase 7
XM_031902710.1	XP_031758570.1	inactive tyrosine-protein kinase 7 isoform X1
XM_031902711.1	XP_031758571.1	inactive tyrosine-protein kinase 7 isoform X2

Pathways from BioSystems

Homologies

	PTK7	XP_002803792.1
Mus musculus (house mouse)	Ptk7	NP_780377.1
Gallus gallus (chicken)	PTK7	NP_001026206.1
Homo sapiens (human)	PTK7	NP_690621.1
Canis lupus familiaris (dog)	PTK7	XP_538929.2
Drosophila melanogaster (fruit fly)	otk	NP_523705.2
Bos taurus (cattle)	PTK7	NP_001179894.1
Xenopus tropicalis (tropical clawed frog)	LOC100487595	XP_002944477.2
Pan troglodytes (chimpanzee)	PTK7	XP_003311345.1
Danio rerio (zebrafish)	ptk7b	XP_005173100.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011489	XP_317813.4

Gene Ontology

The following ptk7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ptk7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa59035	XM_031902710.1 Latest version!	Xenopus tropicalis protein tyrosine kinase 7 (inactive) (ptk7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59036	XM_031902711.1 Latest version!	Xenopus tropicalis protein tyrosine kinase 7 (inactive) (ptk7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ptk7 ( XM_031902710.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758570.1 Xenopus tropicalis protein tyrosine kinase 7 (inactive) (ptk7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59035
Clone ID Related Accession (Same CDS sequence)	XM_031902710.1
Accession Version	XM_031902710.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive tyrosine-protein kinase 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTGA GGGCGCTCTC TGCGCTCATC CTGCTGGTCG TGGCGGGCGC CAGAGCAGCT 
ATCCTGTTCA CTAAGCAGCC GTCCTCCCAG GATGCATTGC ACGGTAGGAG CGCCATACTG 
CGCTGTGAAG TTGAGGATCC TGAGGGGGTG CAGTTTGAGT GGCTGCAGAA TGGGGTGCCA 
GTTCGAGACA CGGAGAGGCG CTACCAGGAG GGCGGGAGTC TGACATTCAC GGCCGTGGAC 
AGGAAACAAG ATGGCGGAAC CTTCCAGTGT GTCGCACGGA ACCCTACAAC AGGGGAAGAG 
GGGAGGAGCA ACGTGGCTTC CTTCAACATT AAGTGGATAG AGAGCGGCGG TGTCACCCTG 
CAGTATCCGG GCAGTGCCAG CGACATCCAG AGCTCCTCTC GGGTCGTGCT GCGATGCCAC 
ATAGATGGGC ACCCTCGCCC AATCTGCCAG TGGTTCAGGG ACGGGACGGC GCTGGTGGAT 
GACGGGCAGG CCACATCGCT AAGCAACAAG GAGCGCACAC TGACCCTGCA CAGCGCGGGC 
CCCGACGACA GCGGCGAGTA TTACTGCTGC GCCCACAATG CGGCCGGCTC CGTCTGCAGC 
AACCGCAACT TCACCCTGAG TATCATAGAT GAGAGCTTCC CGCAGGCCGC GGTGACCCCC 
GAGGATCAGG TGGTGAATAA GAACGAGGAC GCCATGTTTC ACTGCCAGTT CAGCGCCACG 
CCGGCCCCCA CACAGGAATG GTTCTTTGAA GATCAGGTTC CTCTCAGTAA CAAGAGTCGC 
GTGACTGTGT TCCAGAACGG TTCCCTGCTC ATCAGCTCGG TGAGGCCACG TAGCAGTGGG 
GTGTATAGCT GTGTGGGCAC AGGGCATCGT GGCAAGAAGG CTGTTCTCAA GGCCTCTCTG 
CGCCTGGCAG ATATCGATGA AATGCGACCC CTCTCTCCAC AAGTGTTAAC TGCAGACACG 
TTTCAGCGCA TTGCCTGCAA CCGGCCGCAA GGCTTGCCCC CACCTACCGT CTGGTGGGAG 
AGAGATGGAA AGCCGGTCCC TGCTGAGGGC AGAGTTTATC AGGATGGCAC CGAGTTGGTA 
TTCAGACCCA TTACTAAGGA GGATTCGGGC ATTTACATTT GCCATGCTGC CAACAAGGCT 
GGGGAACGCA AACAGGAGCT GCAGATCACT GTGGCGACGG TTCCTGAGTG GCTGGAGAGA 
CCGACAGACA GTCTGATGGA AGAGGGAAAG CCTGGGTTTC TGCACTGTCT CAGCAGAGCG 
TCTCTGGAAC CCAATGTGAC TTGGTACCGT AACACCAACC CAATCGGCAA GGACTCTCGC 
TTTGAGATCT TCCCCAATGG GACCCTAAAG ATCCTGCACG TGGAGGTGTA CGACGGCATT 
ACGTACAGAT GTGTGAGTGC CACCCCTGCT GGCGCCATCG ATGCCCAGGC CCGAGTGACC 
GTACAGGAGA ATCTGAAGTT CACGCCGGTC CCCCGGCCGC AGCAGTGCAT GGAGCTGGAT 
AAGGAGATAA CGGTGCATTG TTCCGCCACG GGGCGCCAGA AGCCCCTTAT ACAGTGGACT 
AAAGGAGATG GCAGTGACCT CCCTGCACAT GTGGAGTCTA AGGCGGGCCT CCTGCACTTT 
CAGCCCGTGA CCCGGAGGGA TGCTGGGAAT TACACCTGTA TTGCCTCTAA CAGCCAGCAG 
GGGGAGATAC GAGCAGCGGT GCAGCTGACT GTAGCAGTTC TGATCAGCTT CAAGATAGAA 
CCAGAGAACA CGACTGTGTA CCAGGGCCAC ACCGCGGTGC TGCACTGCCA GGCAGGGGGC 
GACCCTACCC CTCGCATCCA GTGGCAGAGC AGGGACGTCA TGGTGGATGT CAAGCATTAC 
CCACGGATAC AGATTATGCC AAATGGCTCA TTAGCCATCT ACCGCGTGAG CAACGAGGAT 
GCTGGGAAAT ACACGTGCAT TGCGGGGAAT GCCTGCGACA TTAAGCACCG AGACACGTTC 
CTTTATGTTG TGGACAAGCC ACCAGGGGGC GCCAGCGAGA CGGATTCCCC ATACAAACTC 
ATCCAGACCA TTGTGCTCTC CGTGGTTGCC GCTGTCACTT ACATCATCAT CGTCTTGGGG 
CTCATGTTGT ACTGCAAGAA GCGCAGGAAA GCTAGGAGGC GGGACAAGCC TGAGGGGGAG 
GAGCCAGAGA TGGAGTGCCT AAACGGTGGC ACATTGCTGC AGAATGGCCA GACCACGGCT 
GAGATCCCTG AGGAGGTTCC ACTCACCACA CTGGGCAACA AAAGGCACAG CTCTGGTGAC 
AAGATCACTT TCCCACGTGC CAGTCTGCAC CCTATCACCA CCCTTGGTCG GGGTGAGTTC 
GGGGAGGTGT TCCTGGCTAA GGCCCCCAGT GCAGATTCGG CGGCGGGGGA GTCGGTGGTT 
CTGGTGAAGG CGCTACAGAC CCGGGACGAG CAGCTCCAGA TGGATTTCAG GCGGGAATTG 
GACATGTTCT CCAAACTGAA CCATTCCAAC GTGGTGCGCC TGGTGGGGCA GTGCCGCGAG 
GCAGAACCTC ACTATATGGT TCTGGAATAT GTTGACCTGG GGGATCTGAA GCAGTTCCTA 
CGGATCTCCA GGAGTCGAGA TGAGAAGCCG AAGCCCCTAA GCTCCAAGCA CAAGGTCTCT 
CTCTGCTCTC AGGTGGCTCT GGGGATGGAG CATCTGTCTA ACAGTCGCTT TGTGCACAAG 
GACCTTGCAG CCCGGAACTG TCTCGTCAGT GCCCAGCGGT TGGTGAAGGT CTCTGCCCTG 
GGCCTGAGCA AGGACGTGTA CAGCAGCGAG TACCACCCCC TACGCCAGGC CAAGGTGCCC 
CTACGCTGGA TGCCCCCCGA GGCAGTGCAG GAAGACGACT TCTCCACCAA ATCTGACGTT 
TGGTCCTTTG GGGTGCTAAT GTGGGAGGTC TTCACCCTGG GGGAGCTGCC CTACACCTCT 
CTGCCCGATG AGGAGGTGCT TGCTGGCCTA CAGAACGGCT CCCTGAAACT GGCGGCCCCC 
GAGGGCTGTT CCTCCCGGAT CTACAGACTG ATGCAGCGCT GCTGGGCCCC CAGCCCCAAG 
GACCGACCCT CCTTTAGTGA AATAGTCAAC ACTCTCGGGG ATTTAACTTC CAGCAGCAAA 
ACCCAAGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758570.1
CDS	129..3260
Translation	MGLRALSALILLVVAGARAAILFTKQPSSQDALHGRSAILRCEVEDPEGVQFEWLQNGVPVRDTERRYQEGGSLTFTAVDRKQDGGTFQCVARNPTTGEEGRSNVASFNIKWIESGGVTLQYPGSASDIQSSSRVVLRCHIDGHPRPICQWFRDGTALVDDGQATSLSNKERTLTLHSAGPDDSGEYYCCAHNAAGSVCSNRNFTLSIIDESFPQAAVTPEDQVVNKNEDAMFHCQFSATPAPTQEWFFEDQVPLSNKSRVTVFQNGSLLISSVRPRSSGVYSCVGTGHRGKKAVLKASLRLADIDEMRPLSPQVLTADTFQRIACNRPQGLPPPTVWWERDGKPVPAEGRVYQDGTELVFRPITKEDSGIYICHAANKAGERKQELQITVATVPEWLERPTDSLMEEGKPGFLHCLSRASLEPNVTWYRNTNPIGKDSRFEIFPNGTLKILHVEVYDGITYRCVSATPAGAIDAQARVTVQENLKFTPVPRPQQCMELDKEITVHCSATGRQKPLIQWTKGDGSDLPAHVESKAGLLHFQPVTRRDAGNYTCIASNSQQGEIRAAVQLTVAVLISFKIEPENTTVYQGHTAVLHCQAGGDPTPRIQWQSRDVMVDVKHYPRIQIMPNGSLAIYRVSNEDAGKYTCIAGNACDIKHRDTFLYVVDKPPGGASETDSPYKLIQTIVLSVVAAVTYIIIVLGLMLYCKKRRKARRRDKPEGEEPEMECLNGGTLLQNGQTTAEIPEEVPLTTLGNKRHSSGDKITFPRASLHPITTLGRGEFGEVFLAKAPSADSAAGESVVLVKALQTRDEQLQMDFRRELDMFSKLNHSNVVRLVGQCREAEPHYMVLEYVDLGDLKQFLRISRSRDEKPKPLSSKHKVSLCSQVALGMEHLSNSRFVHKDLAARNCLVSAQRLVKVSALGLSKDVYSSEYHPLRQAKVPLRWMPPEAVQEDDFSTKSDVWSFGVLMWEVFTLGELPYTSLPDEEVLAGLQNGSLKLAAPEGCSSRIYRLMQRCWAPSPKDRPSFSEIVNTLGDLTSSSKTQG

Target ORF information:

RefSeq Version	XM_031902710.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine kinase 7 (inactive) (ptk7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902710.1

ORF Insert Sequence:

ATGGGGCTGA GGGCGCTCTC TGCGCTCATC CTGCTGGTCG TGGCGGGCGC CAGAGCAGCT 
ATCCTGTTCA CTAAGCAGCC GTCCTCCCAG GATGCATTGC ACGGTAGGAG CGCCATACTG 
CGCTGTGAAG TTGAGGATCC TGAGGGGGTG CAGTTTGAGT GGCTGCAGAA TGGGGTGCCA 
GTTCGAGACA CGGAGAGGCG CTACCAGGAG GGCGGGAGTC TGACATTCAC GGCCGTGGAC 
AGGAAACAAG ATGGCGGAAC CTTCCAGTGT GTCGCACGGA ACCCTACAAC AGGGGAAGAG 
GGGAGGAGCA ACGTGGCTTC CTTCAACATT AAGTGGATAG AGAGCGGCGG TGTCACCCTG 
CAGTATCCGG GCAGTGCCAG CGACATCCAG AGCTCCTCTC GGGTCGTGCT GCGATGCCAC 
ATAGATGGGC ACCCTCGCCC AATCTGCCAG TGGTTCAGGG ACGGGACGGC GCTGGTGGAT 
GACGGGCAGG CCACATCGCT AAGCAACAAG GAGCGCACAC TGACCCTGCA CAGCGCGGGC 
CCCGACGACA GCGGCGAGTA TTACTGCTGC GCCCACAATG CGGCCGGCTC CGTCTGCAGC 
AACCGCAACT TCACCCTGAG TATCATAGAT GAGAGCTTCC CGCAGGCCGC GGTGACCCCC 
GAGGATCAGG TGGTGAATAA GAACGAGGAC GCCATGTTTC ACTGCCAGTT CAGCGCCACG 
CCGGCCCCCA CACAGGAATG GTTCTTTGAA GATCAGGTTC CTCTCAGTAA CAAGAGTCGC 
GTGACTGTGT TCCAGAACGG TTCCCTGCTC ATCAGCTCGG TGAGGCCACG TAGCAGTGGG 
GTGTATAGCT GTGTGGGCAC AGGGCATCGT GGCAAGAAGG CTGTTCTCAA GGCCTCTCTG 
CGCCTGGCAG ATATCGATGA AATGCGACCC CTCTCTCCAC AAGTGTTAAC TGCAGACACG 
TTTCAGCGCA TTGCCTGCAA CCGGCCGCAA GGCTTGCCCC CACCTACCGT CTGGTGGGAG 
AGAGATGGAA AGCCGGTCCC TGCTGAGGGC AGAGTTTATC AGGATGGCAC CGAGTTGGTA 
TTCAGACCCA TTACTAAGGA GGATTCGGGC ATTTACATTT GCCATGCTGC CAACAAGGCT 
GGGGAACGCA AACAGGAGCT GCAGATCACT GTGGCGACGG TTCCTGAGTG GCTGGAGAGA 
CCGACAGACA GTCTGATGGA AGAGGGAAAG CCTGGGTTTC TGCACTGTCT CAGCAGAGCG 
TCTCTGGAAC CCAATGTGAC TTGGTACCGT AACACCAACC CAATCGGCAA GGACTCTCGC 
TTTGAGATCT TCCCCAATGG GACCCTAAAG ATCCTGCACG TGGAGGTGTA CGACGGCATT 
ACGTACAGAT GTGTGAGTGC CACCCCTGCT GGCGCCATCG ATGCCCAGGC CCGAGTGACC 
GTACAGGAGA ATCTGAAGTT CACGCCGGTC CCCCGGCCGC AGCAGTGCAT GGAGCTGGAT 
AAGGAGATAA CGGTGCATTG TTCCGCCACG GGGCGCCAGA AGCCCCTTAT ACAGTGGACT 
AAAGGAGATG GCAGTGACCT CCCTGCACAT GTGGAGTCTA AGGCGGGCCT CCTGCACTTT 
CAGCCCGTGA CCCGGAGGGA TGCTGGGAAT TACACCTGTA TTGCCTCTAA CAGCCAGCAG 
GGGGAGATAC GAGCAGCGGT GCAGCTGACT GTAGCAGTTC TGATCAGCTT CAAGATAGAA 
CCAGAGAACA CGACTGTGTA CCAGGGCCAC ACCGCGGTGC TGCACTGCCA GGCAGGGGGC 
GACCCTACCC CTCGCATCCA GTGGCAGAGC AGGGACGTCA TGGTGGATGT CAAGCATTAC 
CCACGGATAC AGATTATGCC AAATGGCTCA TTAGCCATCT ACCGCGTGAG CAACGAGGAT 
GCTGGGAAAT ACACGTGCAT TGCGGGGAAT GCCTGCGACA TTAAGCACCG AGACACGTTC 
CTTTATGTTG TGGACAAGCC ACCAGGGGGC GCCAGCGAGA CGGATTCCCC ATACAAACTC 
ATCCAGACCA TTGTGCTCTC CGTGGTTGCC GCTGTCACTT ACATCATCAT CGTCTTGGGG 
CTCATGTTGT ACTGCAAGAA GCGCAGGAAA GCTAGGAGGC GGGACAAGCC TGAGGGGGAG 
GAGCCAGAGA TGGAGTGCCT AAACGGTGGC ACATTGCTGC AGAATGGCCA GACCACGGCT 
GAGATCCCTG AGGAGGTTCC ACTCACCACA CTGGGCAACA AAAGGCACAG CTCTGGTGAC 
AAGATCACTT TCCCACGTGC CAGTCTGCAC CCTATCACCA CCCTTGGTCG GGGTGAGTTC 
GGGGAGGTGT TCCTGGCTAA GGCCCCCAGT GCAGATTCGG CGGCGGGGGA GTCGGTGGTT 
CTGGTGAAGG CGCTACAGAC CCGGGACGAG CAGCTCCAGA TGGATTTCAG GCGGGAATTG 
GACATGTTCT CCAAACTGAA CCATTCCAAC GTGGTGCGCC TGGTGGGGCA GTGCCGCGAG 
GCAGAACCTC ACTATATGGT TCTGGAATAT GTTGACCTGG GGGATCTGAA GCAGTTCCTA 
CGGATCTCCA GGAGTCGAGA TGAGAAGCCG AAGCCCCTAA GCTCCAAGCA CAAGGTCTCT 
CTCTGCTCTC AGGTGGCTCT GGGGATGGAG CATCTGTCTA ACAGTCGCTT TGTGCACAAG 
GACCTTGCAG CCCGGAACTG TCTCGTCAGT GCCCAGCGGT TGGTGAAGGT CTCTGCCCTG 
GGCCTGAGCA AGGACGTGTA CAGCAGCGAG TACCACCCCC TACGCCAGGC CAAGGTGCCC 
CTACGCTGGA TGCCCCCCGA GGCAGTGCAG GAAGACGACT TCTCCACCAA ATCTGACGTT 
TGGTCCTTTG GGGTGCTAAT GTGGGAGGTC TTCACCCTGG GGGAGCTGCC CTACACCTCT 
CTGCCCGATG AGGAGGTGCT TGCTGGCCTA CAGAACGGCT CCCTGAAACT GGCGGCCCCC 
GAGGGCTGTT CCTCCCGGAT CTACAGACTG ATGCAGCGCT GCTGGGCCCC CAGCCCCAAG 
GACCGACCCT CCTTTAGTGA AATAGTCAAC ACTCTCGGGG ATTTAACTTC CAGCAGCAAA 
ACCCAAGGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ptk7 ( XM_031902711.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758571.1 Xenopus tropicalis protein tyrosine kinase 7 (inactive) (ptk7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa59036
Clone ID Related Accession (Same CDS sequence)	XM_031902711.1
Accession Version	XM_031902711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3111bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	inactive tyrosine-protein kinase 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGAT ACGGCCAGTT GGCGGGCGCC AGAGCAGCTA TCCTGTTCAC TAAGCAGCCG 
TCCTCCCAGG ATGCATTGCA CGGTAGGAGC GCCATACTGC GCTGTGAAGT TGAGGATCCT 
GAGGGGGTGC AGTTTGAGTG GCTGCAGAAT GGGGTGCCAG TTCGAGACAC GGAGAGGCGC 
TACCAGGAGG GCGGGAGTCT GACATTCACG GCCGTGGACA GGAAACAAGA TGGCGGAACC 
TTCCAGTGTG TCGCACGGAA CCCTACAACA GGGGAAGAGG GGAGGAGCAA CGTGGCTTCC 
TTCAACATTA AGTGGATAGA GAGCGGCGGT GTCACCCTGC AGTATCCGGG CAGTGCCAGC 
GACATCCAGA GCTCCTCTCG GGTCGTGCTG CGATGCCACA TAGATGGGCA CCCTCGCCCA 
ATCTGCCAGT GGTTCAGGGA CGGGACGGCG CTGGTGGATG ACGGGCAGGC CACATCGCTA 
AGCAACAAGG AGCGCACACT GACCCTGCAC AGCGCGGGCC CCGACGACAG CGGCGAGTAT 
TACTGCTGCG CCCACAATGC GGCCGGCTCC GTCTGCAGCA ACCGCAACTT CACCCTGAGT 
ATCATAGATG AGAGCTTCCC GCAGGCCGCG GTGACCCCCG AGGATCAGGT GGTGAATAAG 
AACGAGGACG CCATGTTTCA CTGCCAGTTC AGCGCCACGC CGGCCCCCAC ACAGGAATGG 
TTCTTTGAAG ATCAGGTTCC TCTCAGTAAC AAGAGTCGCG TGACTGTGTT CCAGAACGGT 
TCCCTGCTCA TCAGCTCGGT GAGGCCACGT AGCAGTGGGG TGTATAGCTG TGTGGGCACA 
GGGCATCGTG GCAAGAAGGC TGTTCTCAAG GCCTCTCTGC GCCTGGCAGA TATCGATGAA 
ATGCGACCCC TCTCTCCACA AGTGTTAACT GCAGACACGT TTCAGCGCAT TGCCTGCAAC 
CGGCCGCAAG GCTTGCCCCC ACCTACCGTC TGGTGGGAGA GAGATGGAAA GCCGGTCCCT 
GCTGAGGGCA GAGTTTATCA GGATGGCACC GAGTTGGTAT TCAGACCCAT TACTAAGGAG 
GATTCGGGCA TTTACATTTG CCATGCTGCC AACAAGGCTG GGGAACGCAA ACAGGAGCTG 
CAGATCACTG TGGCGACGGT TCCTGAGTGG CTGGAGAGAC CGACAGACAG TCTGATGGAA 
GAGGGAAAGC CTGGGTTTCT GCACTGTCTC AGCAGAGCGT CTCTGGAACC CAATGTGACT 
TGGTACCGTA ACACCAACCC AATCGGCAAG GACTCTCGCT TTGAGATCTT CCCCAATGGG 
ACCCTAAAGA TCCTGCACGT GGAGGTGTAC GACGGCATTA CGTACAGATG TGTGAGTGCC 
ACCCCTGCTG GCGCCATCGA TGCCCAGGCC CGAGTGACCG TACAGGAGAA TCTGAAGTTC 
ACGCCGGTCC CCCGGCCGCA GCAGTGCATG GAGCTGGATA AGGAGATAAC GGTGCATTGT 
TCCGCCACGG GGCGCCAGAA GCCCCTTATA CAGTGGACTA AAGGAGATGG CAGTGACCTC 
CCTGCACATG TGGAGTCTAA GGCGGGCCTC CTGCACTTTC AGCCCGTGAC CCGGAGGGAT 
GCTGGGAATT ACACCTGTAT TGCCTCTAAC AGCCAGCAGG GGGAGATACG AGCAGCGGTG 
CAGCTGACTG TAGCAGTTCT GATCAGCTTC AAGATAGAAC CAGAGAACAC GACTGTGTAC 
CAGGGCCACA CCGCGGTGCT GCACTGCCAG GCAGGGGGCG ACCCTACCCC TCGCATCCAG 
TGGCAGAGCA GGGACGTCAT GGTGGATGTC AAGCATTACC CACGGATACA GATTATGCCA 
AATGGCTCAT TAGCCATCTA CCGCGTGAGC AACGAGGATG CTGGGAAATA CACGTGCATT 
GCGGGGAATG CCTGCGACAT TAAGCACCGA GACACGTTCC TTTATGTTGT GGACAAGCCA 
CCAGGGGGCG CCAGCGAGAC GGATTCCCCA TACAAACTCA TCCAGACCAT TGTGCTCTCC 
GTGGTTGCCG CTGTCACTTA CATCATCATC GTCTTGGGGC TCATGTTGTA CTGCAAGAAG 
CGCAGGAAAG CTAGGAGGCG GGACAAGCCT GAGGGGGAGG AGCCAGAGAT GGAGTGCCTA 
AACGGTGGCA CATTGCTGCA GAATGGCCAG ACCACGGCTG AGATCCCTGA GGAGGTTCCA 
CTCACCACAC TGGGCAACAA AAGGCACAGC TCTGGTGACA AGATCACTTT CCCACGTGCC 
AGTCTGCACC CTATCACCAC CCTTGGTCGG GGTGAGTTCG GGGAGGTGTT CCTGGCTAAG 
GCCCCCAGTG CAGATTCGGC GGCGGGGGAG TCGGTGGTTC TGGTGAAGGC GCTACAGACC 
CGGGACGAGC AGCTCCAGAT GGATTTCAGG CGGGAATTGG ACATGTTCTC CAAACTGAAC 
CATTCCAACG TGGTGCGCCT GGTGGGGCAG TGCCGCGAGG CAGAACCTCA CTATATGGTT 
CTGGAATATG TTGACCTGGG GGATCTGAAG CAGTTCCTAC GGATCTCCAG GAGTCGAGAT 
GAGAAGCCGA AGCCCCTAAG CTCCAAGCAC AAGGTCTCTC TCTGCTCTCA GGTGGCTCTG 
GGGATGGAGC ATCTGTCTAA CAGTCGCTTT GTGCACAAGG ACCTTGCAGC CCGGAACTGT 
CTCGTCAGTG CCCAGCGGTT GGTGAAGGTC TCTGCCCTGG GCCTGAGCAA GGACGTGTAC 
AGCAGCGAGT ACCACCCCCT ACGCCAGGCC AAGGTGCCCC TACGCTGGAT GCCCCCCGAG 
GCAGTGCAGG AAGACGACTT CTCCACCAAA TCTGACGTTT GGTCCTTTGG GGTGCTAATG 
TGGGAGGTCT TCACCCTGGG GGAGCTGCCC TACACCTCTC TGCCCGATGA GGAGGTGCTT 
GCTGGCCTAC AGAACGGCTC CCTGAAACTG GCGGCCCCCG AGGGCTGTTC CTCCCGGATC 
TACAGACTGA TGCAGCGCTG CTGGGCCCCC AGCCCCAAGG ACCGACCCTC CTTTAGTGAA 
ATAGTCAACA CTCTCGGGGA TTTAACTTCC AGCAGCAAAA CCCAAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758571.1
CDS	16..3126
Translation	MSRYGQLAGARAAILFTKQPSSQDALHGRSAILRCEVEDPEGVQFEWLQNGVPVRDTERRYQEGGSLTFTAVDRKQDGGTFQCVARNPTTGEEGRSNVASFNIKWIESGGVTLQYPGSASDIQSSSRVVLRCHIDGHPRPICQWFRDGTALVDDGQATSLSNKERTLTLHSAGPDDSGEYYCCAHNAAGSVCSNRNFTLSIIDESFPQAAVTPEDQVVNKNEDAMFHCQFSATPAPTQEWFFEDQVPLSNKSRVTVFQNGSLLISSVRPRSSGVYSCVGTGHRGKKAVLKASLRLADIDEMRPLSPQVLTADTFQRIACNRPQGLPPPTVWWERDGKPVPAEGRVYQDGTELVFRPITKEDSGIYICHAANKAGERKQELQITVATVPEWLERPTDSLMEEGKPGFLHCLSRASLEPNVTWYRNTNPIGKDSRFEIFPNGTLKILHVEVYDGITYRCVSATPAGAIDAQARVTVQENLKFTPVPRPQQCMELDKEITVHCSATGRQKPLIQWTKGDGSDLPAHVESKAGLLHFQPVTRRDAGNYTCIASNSQQGEIRAAVQLTVAVLISFKIEPENTTVYQGHTAVLHCQAGGDPTPRIQWQSRDVMVDVKHYPRIQIMPNGSLAIYRVSNEDAGKYTCIAGNACDIKHRDTFLYVVDKPPGGASETDSPYKLIQTIVLSVVAAVTYIIIVLGLMLYCKKRRKARRRDKPEGEEPEMECLNGGTLLQNGQTTAEIPEEVPLTTLGNKRHSSGDKITFPRASLHPITTLGRGEFGEVFLAKAPSADSAAGESVVLVKALQTRDEQLQMDFRRELDMFSKLNHSNVVRLVGQCREAEPHYMVLEYVDLGDLKQFLRISRSRDEKPKPLSSKHKVSLCSQVALGMEHLSNSRFVHKDLAARNCLVSAQRLVKVSALGLSKDVYSSEYHPLRQAKVPLRWMPPEAVQEDDFSTKSDVWSFGVLMWEVFTLGELPYTSLPDEEVLAGLQNGSLKLAAPEGCSSRIYRLMQRCWAPSPKDRPSFSEIVNTLGDLTSSSKTQG

Target ORF information:

RefSeq Version	XM_031902711.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis protein tyrosine kinase 7 (inactive) (ptk7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902711.1

ORF Insert Sequence:

ATGAGTAGAT ACGGCCAGTT GGCGGGCGCC AGAGCAGCTA TCCTGTTCAC TAAGCAGCCG 
TCCTCCCAGG ATGCATTGCA CGGTAGGAGC GCCATACTGC GCTGTGAAGT TGAGGATCCT 
GAGGGGGTGC AGTTTGAGTG GCTGCAGAAT GGGGTGCCAG TTCGAGACAC GGAGAGGCGC 
TACCAGGAGG GCGGGAGTCT GACATTCACG GCCGTGGACA GGAAACAAGA TGGCGGAACC 
TTCCAGTGTG TCGCACGGAA CCCTACAACA GGGGAAGAGG GGAGGAGCAA CGTGGCTTCC 
TTCAACATTA AGTGGATAGA GAGCGGCGGT GTCACCCTGC AGTATCCGGG CAGTGCCAGC 
GACATCCAGA GCTCCTCTCG GGTCGTGCTG CGATGCCACA TAGATGGGCA CCCTCGCCCA 
ATCTGCCAGT GGTTCAGGGA CGGGACGGCG CTGGTGGATG ACGGGCAGGC CACATCGCTA 
AGCAACAAGG AGCGCACACT GACCCTGCAC AGCGCGGGCC CCGACGACAG CGGCGAGTAT 
TACTGCTGCG CCCACAATGC GGCCGGCTCC GTCTGCAGCA ACCGCAACTT CACCCTGAGT 
ATCATAGATG AGAGCTTCCC GCAGGCCGCG GTGACCCCCG AGGATCAGGT GGTGAATAAG 
AACGAGGACG CCATGTTTCA CTGCCAGTTC AGCGCCACGC CGGCCCCCAC ACAGGAATGG 
TTCTTTGAAG ATCAGGTTCC TCTCAGTAAC AAGAGTCGCG TGACTGTGTT CCAGAACGGT 
TCCCTGCTCA TCAGCTCGGT GAGGCCACGT AGCAGTGGGG TGTATAGCTG TGTGGGCACA 
GGGCATCGTG GCAAGAAGGC TGTTCTCAAG GCCTCTCTGC GCCTGGCAGA TATCGATGAA 
ATGCGACCCC TCTCTCCACA AGTGTTAACT GCAGACACGT TTCAGCGCAT TGCCTGCAAC 
CGGCCGCAAG GCTTGCCCCC ACCTACCGTC TGGTGGGAGA GAGATGGAAA GCCGGTCCCT 
GCTGAGGGCA GAGTTTATCA GGATGGCACC GAGTTGGTAT TCAGACCCAT TACTAAGGAG 
GATTCGGGCA TTTACATTTG CCATGCTGCC AACAAGGCTG GGGAACGCAA ACAGGAGCTG 
CAGATCACTG TGGCGACGGT TCCTGAGTGG CTGGAGAGAC CGACAGACAG TCTGATGGAA 
GAGGGAAAGC CTGGGTTTCT GCACTGTCTC AGCAGAGCGT CTCTGGAACC CAATGTGACT 
TGGTACCGTA ACACCAACCC AATCGGCAAG GACTCTCGCT TTGAGATCTT CCCCAATGGG 
ACCCTAAAGA TCCTGCACGT GGAGGTGTAC GACGGCATTA CGTACAGATG TGTGAGTGCC 
ACCCCTGCTG GCGCCATCGA TGCCCAGGCC CGAGTGACCG TACAGGAGAA TCTGAAGTTC 
ACGCCGGTCC CCCGGCCGCA GCAGTGCATG GAGCTGGATA AGGAGATAAC GGTGCATTGT 
TCCGCCACGG GGCGCCAGAA GCCCCTTATA CAGTGGACTA AAGGAGATGG CAGTGACCTC 
CCTGCACATG TGGAGTCTAA GGCGGGCCTC CTGCACTTTC AGCCCGTGAC CCGGAGGGAT 
GCTGGGAATT ACACCTGTAT TGCCTCTAAC AGCCAGCAGG GGGAGATACG AGCAGCGGTG 
CAGCTGACTG TAGCAGTTCT GATCAGCTTC AAGATAGAAC CAGAGAACAC GACTGTGTAC 
CAGGGCCACA CCGCGGTGCT GCACTGCCAG GCAGGGGGCG ACCCTACCCC TCGCATCCAG 
TGGCAGAGCA GGGACGTCAT GGTGGATGTC AAGCATTACC CACGGATACA GATTATGCCA 
AATGGCTCAT TAGCCATCTA CCGCGTGAGC AACGAGGATG CTGGGAAATA CACGTGCATT 
GCGGGGAATG CCTGCGACAT TAAGCACCGA GACACGTTCC TTTATGTTGT GGACAAGCCA 
CCAGGGGGCG CCAGCGAGAC GGATTCCCCA TACAAACTCA TCCAGACCAT TGTGCTCTCC 
GTGGTTGCCG CTGTCACTTA CATCATCATC GTCTTGGGGC TCATGTTGTA CTGCAAGAAG 
CGCAGGAAAG CTAGGAGGCG GGACAAGCCT GAGGGGGAGG AGCCAGAGAT GGAGTGCCTA 
AACGGTGGCA CATTGCTGCA GAATGGCCAG ACCACGGCTG AGATCCCTGA GGAGGTTCCA 
CTCACCACAC TGGGCAACAA AAGGCACAGC TCTGGTGACA AGATCACTTT CCCACGTGCC 
AGTCTGCACC CTATCACCAC CCTTGGTCGG GGTGAGTTCG GGGAGGTGTT CCTGGCTAAG 
GCCCCCAGTG CAGATTCGGC GGCGGGGGAG TCGGTGGTTC TGGTGAAGGC GCTACAGACC 
CGGGACGAGC AGCTCCAGAT GGATTTCAGG CGGGAATTGG ACATGTTCTC CAAACTGAAC 
CATTCCAACG TGGTGCGCCT GGTGGGGCAG TGCCGCGAGG CAGAACCTCA CTATATGGTT 
CTGGAATATG TTGACCTGGG GGATCTGAAG CAGTTCCTAC GGATCTCCAG GAGTCGAGAT 
GAGAAGCCGA AGCCCCTAAG CTCCAAGCAC AAGGTCTCTC TCTGCTCTCA GGTGGCTCTG 
GGGATGGAGC ATCTGTCTAA CAGTCGCTTT GTGCACAAGG ACCTTGCAGC CCGGAACTGT 
CTCGTCAGTG CCCAGCGGTT GGTGAAGGTC TCTGCCCTGG GCCTGAGCAA GGACGTGTAC 
AGCAGCGAGT ACCACCCCCT ACGCCAGGCC AAGGTGCCCC TACGCTGGAT GCCCCCCGAG 
GCAGTGCAGG AAGACGACTT CTCCACCAAA TCTGACGTTT GGTCCTTTGG GGTGCTAATG 
TGGGAGGTCT TCACCCTGGG GGAGCTGCCC TACACCTCTC TGCCCGATGA GGAGGTGCTT 
GCTGGCCTAC AGAACGGCTC CCTGAAACTG GCGGCCCCCG AGGGCTGTTC CTCCCGGATC 
TACAGACTGA TGCAGCGCTG CTGGGCCCCC AGCCCCAAGG ACCGACCCTC CTTTAGTGAA 
ATAGTCAACA CTCTCGGGGA TTTAACTTCC AGCAGCAAAA CCCAAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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ptk7 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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